ribolock ribonuclease inhibitor Search Results


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  • 99
    Thermo Fisher ribolock ribonuclease rnase inhibitor
    Ribolock Ribonuclease Rnase Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribolock ribonuclease rnase inhibitor/product/Thermo Fisher
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    ribolock ribonuclease rnase inhibitor - by Bioz Stars, 2020-07
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    99
    Thermo Fisher ribolock ribonuclease inhibitor
    <t>RNase</t> protection assay (nondenaturing agarose gel electrophoresis) of the siRNA–G4NH2 dendriplexes (N/P 5) as a function of the RNase A concentration. Dendriplexes incubated in presence (+) or absence (−) of the treatments: RNase A (0.35, 0.7, 1.0, 1.5, and 3.5 μg per 1 μg siRNA, in lanes 4–7 , 8–11 , 12–15 , 16–19 , 20–23 , respectively) for 6 h at 37 °C, followed by 1 μL (40 U) <t>RiboLock</t> RNase inhibitor for 30 min at 37 °C to block RNase activity, and heparin (455 U per 1 μg siRNA) for 30 min at 37 °C to dissociate the siRNA from the dendrimer. Aqueous medium: TE buffer 1X pH 8. Untreated siRNA control (250 ng) in lane 1 , after incubation with heparin ( lane 2 ) and 0.35 μg RNase A per 1 μg siRNA ( lane 3 ).
    Ribolock Ribonuclease Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 562 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribolock ribonuclease inhibitor/product/Thermo Fisher
    Average 99 stars, based on 562 article reviews
    Price from $9.99 to $1999.99
    ribolock ribonuclease inhibitor - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    92
    new england biolabs ribolock ribonuclease inhibitor
    <t>RNase</t> protection assay (nondenaturing agarose gel electrophoresis) of the siRNA–G4NH2 dendriplexes (N/P 5) as a function of the RNase A concentration. Dendriplexes incubated in presence (+) or absence (−) of the treatments: RNase A (0.35, 0.7, 1.0, 1.5, and 3.5 μg per 1 μg siRNA, in lanes 4–7 , 8–11 , 12–15 , 16–19 , 20–23 , respectively) for 6 h at 37 °C, followed by 1 μL (40 U) <t>RiboLock</t> RNase inhibitor for 30 min at 37 °C to block RNase activity, and heparin (455 U per 1 μg siRNA) for 30 min at 37 °C to dissociate the siRNA from the dendrimer. Aqueous medium: TE buffer 1X pH 8. Untreated siRNA control (250 ng) in lane 1 , after incubation with heparin ( lane 2 ) and 0.35 μg RNase A per 1 μg siRNA ( lane 3 ).
    Ribolock Ribonuclease Inhibitor, supplied by new england biolabs, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribolock ribonuclease inhibitor/product/new england biolabs
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    ribolock ribonuclease inhibitor - by Bioz Stars, 2020-07
    92/100 stars
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    97
    Thermo Fisher ribonuclease inhibitor
    <t>RNase</t> protection assay (nondenaturing agarose gel electrophoresis) of the siRNA–G4NH2 dendriplexes (N/P 5) as a function of the RNase A concentration. Dendriplexes incubated in presence (+) or absence (−) of the treatments: RNase A (0.35, 0.7, 1.0, 1.5, and 3.5 μg per 1 μg siRNA, in lanes 4–7 , 8–11 , 12–15 , 16–19 , 20–23 , respectively) for 6 h at 37 °C, followed by 1 μL (40 U) <t>RiboLock</t> RNase inhibitor for 30 min at 37 °C to block RNase activity, and heparin (455 U per 1 μg siRNA) for 30 min at 37 °C to dissociate the siRNA from the dendrimer. Aqueous medium: TE buffer 1X pH 8. Untreated siRNA control (250 ng) in lane 1 , after incubation with heparin ( lane 2 ) and 0.35 μg RNase A per 1 μg siRNA ( lane 3 ).
    Ribonuclease Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonuclease inhibitor/product/Thermo Fisher
    Average 97 stars, based on 1427 article reviews
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    ribonuclease inhibitor - by Bioz Stars, 2020-07
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    tiangen biotech co ribolock rnase inhibitors
    <t>RNase</t> protection assay (nondenaturing agarose gel electrophoresis) of the siRNA–G4NH2 dendriplexes (N/P 5) as a function of the RNase A concentration. Dendriplexes incubated in presence (+) or absence (−) of the treatments: RNase A (0.35, 0.7, 1.0, 1.5, and 3.5 μg per 1 μg siRNA, in lanes 4–7 , 8–11 , 12–15 , 16–19 , 20–23 , respectively) for 6 h at 37 °C, followed by 1 μL (40 U) <t>RiboLock</t> RNase inhibitor for 30 min at 37 °C to block RNase activity, and heparin (455 U per 1 μg siRNA) for 30 min at 37 °C to dissociate the siRNA from the dendrimer. Aqueous medium: TE buffer 1X pH 8. Untreated siRNA control (250 ng) in lane 1 , after incubation with heparin ( lane 2 ) and 0.35 μg RNase A per 1 μg siRNA ( lane 3 ).
    Ribolock Rnase Inhibitors, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribolock rnase inhibitors/product/tiangen biotech co
    Average 90 stars, based on 6 article reviews
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    ribolock rnase inhibitors - by Bioz Stars, 2020-07
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    92
    Fisher Scientific ribolock rnase inhibitor
    <t>RNase</t> protection assay (nondenaturing agarose gel electrophoresis) of the siRNA–G4NH2 dendriplexes (N/P 5) as a function of the RNase A concentration. Dendriplexes incubated in presence (+) or absence (−) of the treatments: RNase A (0.35, 0.7, 1.0, 1.5, and 3.5 μg per 1 μg siRNA, in lanes 4–7 , 8–11 , 12–15 , 16–19 , 20–23 , respectively) for 6 h at 37 °C, followed by 1 μL (40 U) <t>RiboLock</t> RNase inhibitor for 30 min at 37 °C to block RNase activity, and heparin (455 U per 1 μg siRNA) for 30 min at 37 °C to dissociate the siRNA from the dendrimer. Aqueous medium: TE buffer 1X pH 8. Untreated siRNA control (250 ng) in lane 1 , after incubation with heparin ( lane 2 ) and 0.35 μg RNase A per 1 μg siRNA ( lane 3 ).
    Ribolock Rnase Inhibitor, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribolock rnase inhibitor/product/Fisher Scientific
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    ribolock rnase inhibitor - by Bioz Stars, 2020-07
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    90
    Roche ribolock rnase inhibitor
    <t>RNase</t> protection assay (nondenaturing agarose gel electrophoresis) of the siRNA–G4NH2 dendriplexes (N/P 5) as a function of the RNase A concentration. Dendriplexes incubated in presence (+) or absence (−) of the treatments: RNase A (0.35, 0.7, 1.0, 1.5, and 3.5 μg per 1 μg siRNA, in lanes 4–7 , 8–11 , 12–15 , 16–19 , 20–23 , respectively) for 6 h at 37 °C, followed by 1 μL (40 U) <t>RiboLock</t> RNase inhibitor for 30 min at 37 °C to block RNase activity, and heparin (455 U per 1 μg siRNA) for 30 min at 37 °C to dissociate the siRNA from the dendrimer. Aqueous medium: TE buffer 1X pH 8. Untreated siRNA control (250 ng) in lane 1 , after incubation with heparin ( lane 2 ) and 0.35 μg RNase A per 1 μg siRNA ( lane 3 ).
    Ribolock Rnase Inhibitor, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribolock rnase inhibitor/product/Roche
    Average 90 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    ribolock rnase inhibitor - by Bioz Stars, 2020-07
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    Image Search Results


    RNase protection assay (nondenaturing agarose gel electrophoresis) of the siRNA–G4NH2 dendriplexes (N/P 5) as a function of the RNase A concentration. Dendriplexes incubated in presence (+) or absence (−) of the treatments: RNase A (0.35, 0.7, 1.0, 1.5, and 3.5 μg per 1 μg siRNA, in lanes 4–7 , 8–11 , 12–15 , 16–19 , 20–23 , respectively) for 6 h at 37 °C, followed by 1 μL (40 U) RiboLock RNase inhibitor for 30 min at 37 °C to block RNase activity, and heparin (455 U per 1 μg siRNA) for 30 min at 37 °C to dissociate the siRNA from the dendrimer. Aqueous medium: TE buffer 1X pH 8. Untreated siRNA control (250 ng) in lane 1 , after incubation with heparin ( lane 2 ) and 0.35 μg RNase A per 1 μg siRNA ( lane 3 ).

    Journal: Molecular Pharmaceutics

    Article Title: Poly(amidoamine) Dendrimer Nanocarriers and Their Aerosol Formulations for siRNA Delivery to the Lung Epithelium

    doi: 10.1021/mp4006358

    Figure Lengend Snippet: RNase protection assay (nondenaturing agarose gel electrophoresis) of the siRNA–G4NH2 dendriplexes (N/P 5) as a function of the RNase A concentration. Dendriplexes incubated in presence (+) or absence (−) of the treatments: RNase A (0.35, 0.7, 1.0, 1.5, and 3.5 μg per 1 μg siRNA, in lanes 4–7 , 8–11 , 12–15 , 16–19 , 20–23 , respectively) for 6 h at 37 °C, followed by 1 μL (40 U) RiboLock RNase inhibitor for 30 min at 37 °C to block RNase activity, and heparin (455 U per 1 μg siRNA) for 30 min at 37 °C to dissociate the siRNA from the dendrimer. Aqueous medium: TE buffer 1X pH 8. Untreated siRNA control (250 ng) in lane 1 , after incubation with heparin ( lane 2 ) and 0.35 μg RNase A per 1 μg siRNA ( lane 3 ).

    Article Snippet: RiboLock RNase Inhibitor (RI, EO0381, 40 U × μL–1 ) was purchased from Thermo Scientific (part of Thermo Fisher Scientific, Waltham, MA, U.S.A.).

    Techniques: Rnase Protection Assay, Agarose Gel Electrophoresis, Concentration Assay, Incubation, Blocking Assay, Activity Assay

    RNase protection assay (non-denaturing agarose gel electrophoresis) of the siRNA–G4NH2 dendriplexes as a function of the N/P ratio. Dendriplexes incubated in the absence (−) or presence (+) of the treatments: RNase A (0.162 μg per 1 μg siRNA) for 6 h at 37 °C, followed by 1 μL (40 U) RiboLock RNase inhibitor for 30 min at 37 °C to block RNase activity, and heparin (455 U per 1 μg siRNA) for 30 min at 37 °C to dissociate the siRNA from the dendrimer. Aqueous medium: TE buffer 1X pH 8. Untreated siRNA control (300 ng) before ( lane 1 ) and after ( lane 2 ) incubation with RNase A.

    Journal: Molecular Pharmaceutics

    Article Title: Poly(amidoamine) Dendrimer Nanocarriers and Their Aerosol Formulations for siRNA Delivery to the Lung Epithelium

    doi: 10.1021/mp4006358

    Figure Lengend Snippet: RNase protection assay (non-denaturing agarose gel electrophoresis) of the siRNA–G4NH2 dendriplexes as a function of the N/P ratio. Dendriplexes incubated in the absence (−) or presence (+) of the treatments: RNase A (0.162 μg per 1 μg siRNA) for 6 h at 37 °C, followed by 1 μL (40 U) RiboLock RNase inhibitor for 30 min at 37 °C to block RNase activity, and heparin (455 U per 1 μg siRNA) for 30 min at 37 °C to dissociate the siRNA from the dendrimer. Aqueous medium: TE buffer 1X pH 8. Untreated siRNA control (300 ng) before ( lane 1 ) and after ( lane 2 ) incubation with RNase A.

    Article Snippet: RiboLock RNase Inhibitor (RI, EO0381, 40 U × μL–1 ) was purchased from Thermo Scientific (part of Thermo Fisher Scientific, Waltham, MA, U.S.A.).

    Techniques: Rnase Protection Assay, Agarose Gel Electrophoresis, Incubation, Blocking Assay, Activity Assay