rhodamine 6g Search Results


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  • 99
    Millipore rhodamine6g
    Interferogram after removing dc component of 6μm yellow green fluorescent bead (a), Rhodamine <t>6G</t> solution (b), 4μm red fluorescent bead (c) and its the processed spectrum are (d), (e) and (f). Green curve in spectral graph is the reference
    Rhodamine6g, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore rhodamine 6g
    (A) Linear relationship of Raman intensity with different concentrations of target molecules, including Nile red, <t>rhodamine</t> 6G, and crystal violet, in their individual detection. (B) Multiplexed Raman spectra for triple, double as well as individual detections of Nile red, rhodamine 6G, and crystal violet. (C) The reproducibility of the Raman intensity of Nile red at 591 cm −1 from ten randomly different-batched self-assemblies of 80 nm AuNPs coated with P3 with the concentration of 10 −10 g mL −1 .
    Rhodamine 6g, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore rh 6g
    Rh transport and binding by Cdr1p and Cdr2p. (A) <t>Rh</t> 6G resistance profile of TY310 cells transformed with p425GPD-CDR1 (squares), p425GPD-CDR2 (circles), or p425GPD (triangles) as determined by liquid microdilution assay. Values represent the means ± standard deviations of three independent experiments performed in duplicate. (B) Rh 6G efflux activities of the TY310 transformants mentioned in panel A. Extracellular Rh 6G fluorescence was measured by spectrofluorometry and normalized for the amount of cells present in each sample, as determined by measurement of the OD 600 . Values represent the means ± standard deviations of three independent experiments. (C) Photoaffinity labeling of Cdr1p and Cdr2p with IAARh123. Left panel, total membrane proteins (50 μg) from the transformants described above were incubated with 0.5 μM IAARh123, UV cross-linked, and separated by SDS-PAGE; right panel, total membrane proteins (300 μg) were incubated with 1 μM IAARh123, UV cross-linked, and immunoprecipitated with the anti-Cdrp antibody. The immune complexes were resolved by SDS-PAGE. The gels were dried and exposed to Kodak BioMax MS films with two intensifying screens at −80°C for 4 h (left panel) or 5 days (right panel). The results shown are representative of those of at least three independent experiments. The positions of the molecular mass standards (in kilodaltons) are indicated on the left. CTL, control.
    Rh 6g, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Active Motif rhodamine 6g
    Rh transport and binding by Cdr1p and Cdr2p. (A) <t>Rh</t> 6G resistance profile of TY310 cells transformed with p425GPD-CDR1 (squares), p425GPD-CDR2 (circles), or p425GPD (triangles) as determined by liquid microdilution assay. Values represent the means ± standard deviations of three independent experiments performed in duplicate. (B) Rh 6G efflux activities of the TY310 transformants mentioned in panel A. Extracellular Rh 6G fluorescence was measured by spectrofluorometry and normalized for the amount of cells present in each sample, as determined by measurement of the OD 600 . Values represent the means ± standard deviations of three independent experiments. (C) Photoaffinity labeling of Cdr1p and Cdr2p with IAARh123. Left panel, total membrane proteins (50 μg) from the transformants described above were incubated with 0.5 μM IAARh123, UV cross-linked, and separated by SDS-PAGE; right panel, total membrane proteins (300 μg) were incubated with 1 μM IAARh123, UV cross-linked, and immunoprecipitated with the anti-Cdrp antibody. The immune complexes were resolved by SDS-PAGE. The gels were dried and exposed to Kodak BioMax MS films with two intensifying screens at −80°C for 4 h (left panel) or 5 days (right panel). The results shown are representative of those of at least three independent experiments. The positions of the molecular mass standards (in kilodaltons) are indicated on the left. CTL, control.
    Rhodamine 6g, supplied by Active Motif, used in various techniques. Bioz Stars score: 84/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher rhodamine 6g
    In vivo assessment of gingival vascular permeability and leukocyte margination. <t>Rhodamine</t> 6G (R6G; leukocyte labeling) and FITC– dextran (vasculature visualization and tracer molecule) or FITC–LY6G (PMN labeling) were injected intravenously, and intravital microscopy was performed at a controlled body temperature as specified in the Material and methods section. The black arrows indicate the outer side of the vessel wall. (A) The FITC–dextran is retained in the gingival vasculature of a WT mouse. (B) Increased FITC–dextran extravasation in Akita gingiva within the same time period. (C) Rhodamine 6G-positive leukocytes inside gingival postcapillary venules. The FITC–dextran (green) and R6G (red) images were merged offline to obtain this picture. (D) Overlapping images of R6G-positive (red) and LY6G-positive (green) cells indicate that the majority of leukocytes 2 h after tumor necrosis factor α (TNFα) stimulation are polymorphonuclear leukocytes (PMNs; yellow). The white arrows indicate intravascular leukocytes as follows: 1,rolling leukocyte (red); 2, rolling PMN (green); and 3, attached PMN (yellow). Scale bar represents 50 μm; original magnification ×400.
    Rhodamine 6g, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avanti Polar rhodamine 6g
    In vivo assessment of gingival vascular permeability and leukocyte margination. <t>Rhodamine</t> 6G (R6G; leukocyte labeling) and FITC– dextran (vasculature visualization and tracer molecule) or FITC–LY6G (PMN labeling) were injected intravenously, and intravital microscopy was performed at a controlled body temperature as specified in the Material and methods section. The black arrows indicate the outer side of the vessel wall. (A) The FITC–dextran is retained in the gingival vasculature of a WT mouse. (B) Increased FITC–dextran extravasation in Akita gingiva within the same time period. (C) Rhodamine 6G-positive leukocytes inside gingival postcapillary venules. The FITC–dextran (green) and R6G (red) images were merged offline to obtain this picture. (D) Overlapping images of R6G-positive (red) and LY6G-positive (green) cells indicate that the majority of leukocytes 2 h after tumor necrosis factor α (TNFα) stimulation are polymorphonuclear leukocytes (PMNs; yellow). The white arrows indicate intravascular leukocytes as follows: 1,rolling leukocyte (red); 2, rolling PMN (green); and 3, attached PMN (yellow). Scale bar represents 50 μm; original magnification ×400.
    Rhodamine 6g, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tokyo Chemical Industry rhodamine 6g
    Time course of the remaining amounts of <t>rhodamine</t> 6G (red) and fluorescein (green) in a dialysis cartridge.
    Rhodamine 6g, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fluka Chemie rhodamine 6g
    Single molecule detection of 10 −10 M <t>rhodamine</t> 6G (in agarose) in 4 time intervals of 100 ms each (a–d) including a 3D plot (e) (TIR laser excitation at λ= 514 nm; fluorescence measured at λ ≥ 530 nm; image size: 60 μm ×90 μm).
    Rhodamine 6g, supplied by Fluka Chemie, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific rhodamine 6g
    Time dependent fate of <t>Rhodamine-6G</t> labeled CAP nanoparticles (embedded in thermosensitive gel) in HeLa cells after translocation through transwell permeable supports ( n = 3).
    Rhodamine 6g, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sinopharm rhodamine 6g
    Time dependent fate of <t>Rhodamine-6G</t> labeled CAP nanoparticles (embedded in thermosensitive gel) in HeLa cells after translocation through transwell permeable supports ( n = 3).
    Rhodamine 6g, supplied by Sinopharm, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher standard rhodamine 6g
    Time dependent fate of <t>Rhodamine-6G</t> labeled CAP nanoparticles (embedded in thermosensitive gel) in HeLa cells after translocation through transwell permeable supports ( n = 3).
    Standard Rhodamine 6g, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ventana Medical discovery rhodamine 6g kit
    Time dependent fate of <t>Rhodamine-6G</t> labeled CAP nanoparticles (embedded in thermosensitive gel) in HeLa cells after translocation through transwell permeable supports ( n = 3).
    Discovery Rhodamine 6g Kit, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher rhodamine 6g fluorescent dye
    Time dependent fate of <t>Rhodamine-6G</t> labeled CAP nanoparticles (embedded in thermosensitive gel) in HeLa cells after translocation through transwell permeable supports ( n = 3).
    Rhodamine 6g Fluorescent Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Kodak Corp rhodamine 6g
    Photon burst-size histograms, with and without the Poisson time-filter treatment, in green and red, respectively. ( a ) The native receptor after 2 min in the chamber. ( b ) Rhodamine 6G; the burst size is in units of number of photon counts.
    Rhodamine 6g, supplied by Kodak Corp, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rhodamine 6g chloride
    Photon burst-size histograms, with and without the Poisson time-filter treatment, in green and red, respectively. ( a ) The native receptor after 2 min in the chamber. ( b ) Rhodamine 6G; the burst size is in units of number of photon counts.
    Rhodamine 6g Chloride, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co rhodamine 6g
    Manipulation of F 1  rotor motion by optical and chemical inputs . Sequential images of a rotating beads before and after a pulse (10 sec) of high intensity white light illumination (white bar) in the presence (A) or absence (B) of rhodamine 6G. (C) Rotating beads in the presence of rhodamine 6G, but without light pulse.
    Rhodamine 6g, supplied by Merck & Co, used in various techniques. Bioz Stars score: 84/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Aladdin Bio-Chem rhodamine 6g
    Manipulation of F 1  rotor motion by optical and chemical inputs . Sequential images of a rotating beads before and after a pulse (10 sec) of high intensity white light illumination (white bar) in the presence (A) or absence (B) of rhodamine 6G. (C) Rotating beads in the presence of rhodamine 6G, but without light pulse.
    Rhodamine 6g, supplied by Shanghai Aladdin Bio-Chem, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rhodamine 6g
    Manipulation of F 1  rotor motion by optical and chemical inputs . Sequential images of a rotating beads before and after a pulse (10 sec) of high intensity white light illumination (white bar) in the presence (A) or absence (B) of rhodamine 6G. (C) Rotating beads in the presence of rhodamine 6G, but without light pulse.
    Rhodamine 6g, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    J&K Scientific rhodamine 6g
    ( a ) Transmission electron microscopy (TEM) image of 30 nm colloidal Au nanoparticles (NPs); ( b ) surface-enhanced Raman scattering (SERS) spectra of 10 −3 M <t>rhodamine</t> 6G (R6G) using colloidal Au NPs with different sizes (10, 20, 30, 40, and 50 nm); ( c ) SERS spectra of R6G with concentrations from 10 −17 to 10 −2 M (bottom to top, concentration successively increasing by a factor of 10) using 30 nm colloidal Au NPs. The intensity is multiplied five times for 10 −11 to 10 −7 M R6G and 10 times for 10 −17 to 10 −12 M R6G; ( d ) Linear relationship between log I of the band peak at 1361 cm −1 and log C based on the SERS data of R6G in ( c ).
    Rhodamine 6g, supplied by J&K Scientific, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    AnaSpec rhodamine 6g
    ( a ) Transmission electron microscopy (TEM) image of 30 nm colloidal Au nanoparticles (NPs); ( b ) surface-enhanced Raman scattering (SERS) spectra of 10 −3 M <t>rhodamine</t> 6G (R6G) using colloidal Au NPs with different sizes (10, 20, 30, 40, and 50 nm); ( c ) SERS spectra of R6G with concentrations from 10 −17 to 10 −2 M (bottom to top, concentration successively increasing by a factor of 10) using 30 nm colloidal Au NPs. The intensity is multiplied five times for 10 −11 to 10 −7 M R6G and 10 times for 10 −17 to 10 −12 M R6G; ( d ) Linear relationship between log I of the band peak at 1361 cm −1 and log C based on the SERS data of R6G in ( c ).
    Rhodamine 6g, supplied by AnaSpec, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    EM Science Inc rhodamine 6g
    ( a ) Transmission electron microscopy (TEM) image of 30 nm colloidal Au nanoparticles (NPs); ( b ) surface-enhanced Raman scattering (SERS) spectra of 10 −3 M <t>rhodamine</t> 6G (R6G) using colloidal Au NPs with different sizes (10, 20, 30, 40, and 50 nm); ( c ) SERS spectra of R6G with concentrations from 10 −17 to 10 −2 M (bottom to top, concentration successively increasing by a factor of 10) using 30 nm colloidal Au NPs. The intensity is multiplied five times for 10 −11 to 10 −7 M R6G and 10 times for 10 −17 to 10 −12 M R6G; ( d ) Linear relationship between log I of the band peak at 1361 cm −1 and log C based on the SERS data of R6G in ( c ).
    Rhodamine 6g, supplied by EM Science Inc, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FUJIFILM rhodamine 6g
    ( a ) Transmission electron microscopy (TEM) image of 30 nm colloidal Au nanoparticles (NPs); ( b ) surface-enhanced Raman scattering (SERS) spectra of 10 −3 M <t>rhodamine</t> 6G (R6G) using colloidal Au NPs with different sizes (10, 20, 30, 40, and 50 nm); ( c ) SERS spectra of R6G with concentrations from 10 −17 to 10 −2 M (bottom to top, concentration successively increasing by a factor of 10) using 30 nm colloidal Au NPs. The intensity is multiplied five times for 10 −11 to 10 −7 M R6G and 10 times for 10 −17 to 10 −12 M R6G; ( d ) Linear relationship between log I of the band peak at 1361 cm −1 and log C based on the SERS data of R6G in ( c ).
    Rhodamine 6g, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA rhodamine 6g
    ( a ) Transmission electron microscopy (TEM) image of 30 nm colloidal Au nanoparticles (NPs); ( b ) surface-enhanced Raman scattering (SERS) spectra of 10 −3 M <t>rhodamine</t> 6G (R6G) using colloidal Au NPs with different sizes (10, 20, 30, 40, and 50 nm); ( c ) SERS spectra of R6G with concentrations from 10 −17 to 10 −2 M (bottom to top, concentration successively increasing by a factor of 10) using 30 nm colloidal Au NPs. The intensity is multiplied five times for 10 −11 to 10 −7 M R6G and 10 times for 10 −17 to 10 −12 M R6G; ( d ) Linear relationship between log I of the band peak at 1361 cm −1 and log C based on the SERS data of R6G in ( c ).
    Rhodamine 6g, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rhodamine 6g dye laser
    ( a ) Transmission electron microscopy (TEM) image of 30 nm colloidal Au nanoparticles (NPs); ( b ) surface-enhanced Raman scattering (SERS) spectra of 10 −3 M <t>rhodamine</t> 6G (R6G) using colloidal Au NPs with different sizes (10, 20, 30, 40, and 50 nm); ( c ) SERS spectra of R6G with concentrations from 10 −17 to 10 −2 M (bottom to top, concentration successively increasing by a factor of 10) using 30 nm colloidal Au NPs. The intensity is multiplied five times for 10 −11 to 10 −7 M R6G and 10 times for 10 −17 to 10 −12 M R6G; ( d ) Linear relationship between log I of the band peak at 1361 cm −1 and log C based on the SERS data of R6G in ( c ).
    Rhodamine 6g Dye Laser, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tokyo Chemical Industry rhodamine 6g r 6g
    ( a ) Transmission electron microscopy (TEM) image of 30 nm colloidal Au nanoparticles (NPs); ( b ) surface-enhanced Raman scattering (SERS) spectra of 10 −3 M <t>rhodamine</t> 6G (R6G) using colloidal Au NPs with different sizes (10, 20, 30, 40, and 50 nm); ( c ) SERS spectra of R6G with concentrations from 10 −17 to 10 −2 M (bottom to top, concentration successively increasing by a factor of 10) using 30 nm colloidal Au NPs. The intensity is multiplied five times for 10 −11 to 10 −7 M R6G and 10 times for 10 −17 to 10 −12 M R6G; ( d ) Linear relationship between log I of the band peak at 1361 cm −1 and log C based on the SERS data of R6G in ( c ).
    Rhodamine 6g R 6g, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore fluorescent dye rhodamine 6g
    ( a ) Transmission electron microscopy (TEM) image of 30 nm colloidal Au nanoparticles (NPs); ( b ) surface-enhanced Raman scattering (SERS) spectra of 10 −3 M <t>rhodamine</t> 6G (R6G) using colloidal Au NPs with different sizes (10, 20, 30, 40, and 50 nm); ( c ) SERS spectra of R6G with concentrations from 10 −17 to 10 −2 M (bottom to top, concentration successively increasing by a factor of 10) using 30 nm colloidal Au NPs. The intensity is multiplied five times for 10 −11 to 10 −7 M R6G and 10 times for 10 −17 to 10 −12 M R6G; ( d ) Linear relationship between log I of the band peak at 1361 cm −1 and log C based on the SERS data of R6G in ( c ).
    Fluorescent Dye Rhodamine 6g, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rhodamine 6g dye
    Activated human platelets support WBCs homing to subiliac PLN in L-selectin–deficient mice. WBCs were fluorescently labeled in situ using <t>rhodamine</t> 6G and recorded during their passage through single HEV. ( A ) Digitized intravital fluorescence micrograph of a typical HEV in an L-selectin–deficient mouse. Relatively few blood-borne cells ( arrows ) interacted with the vascular wall over a 45-min control period ( 0 min ). ( B ) Intraarterial injection of TRAP-stimulated human platelets over a 10-min period resulted in rolling and accumulation of sticking endogenous WBCs ( 10 min ). ( C ) A significant number of stuck cells subsequently transmigrated into the node ( 45 min ). ( D ) LFA-1 (CD11a) receptor blockade abrogates firm adhesion of L-selectin–deficient WBCs delivered to HEV by platelets. The percentage of rolling cells that became firmly adherent for a mininum of 30 s (sticking fraction) was determined in the presence or absence of LFA-1 mAb TIB 213. The data shown represent the mean ± SD of seven venules in three animals.
    Rhodamine 6g Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BASF rhodamine 6g dye
    Activated human platelets support WBCs homing to subiliac PLN in L-selectin–deficient mice. WBCs were fluorescently labeled in situ using <t>rhodamine</t> 6G and recorded during their passage through single HEV. ( A ) Digitized intravital fluorescence micrograph of a typical HEV in an L-selectin–deficient mouse. Relatively few blood-borne cells ( arrows ) interacted with the vascular wall over a 45-min control period ( 0 min ). ( B ) Intraarterial injection of TRAP-stimulated human platelets over a 10-min period resulted in rolling and accumulation of sticking endogenous WBCs ( 10 min ). ( C ) A significant number of stuck cells subsequently transmigrated into the node ( 45 min ). ( D ) LFA-1 (CD11a) receptor blockade abrogates firm adhesion of L-selectin–deficient WBCs delivered to HEV by platelets. The percentage of rolling cells that became firmly adherent for a mininum of 30 s (sticking fraction) was determined in the presence or absence of LFA-1 mAb TIB 213. The data shown represent the mean ± SD of seven venules in three animals.
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    Merck KGaA dye rhodamine 6g
    Activated human platelets support WBCs homing to subiliac PLN in L-selectin–deficient mice. WBCs were fluorescently labeled in situ using <t>rhodamine</t> 6G and recorded during their passage through single HEV. ( A ) Digitized intravital fluorescence micrograph of a typical HEV in an L-selectin–deficient mouse. Relatively few blood-borne cells ( arrows ) interacted with the vascular wall over a 45-min control period ( 0 min ). ( B ) Intraarterial injection of TRAP-stimulated human platelets over a 10-min period resulted in rolling and accumulation of sticking endogenous WBCs ( 10 min ). ( C ) A significant number of stuck cells subsequently transmigrated into the node ( 45 min ). ( D ) LFA-1 (CD11a) receptor blockade abrogates firm adhesion of L-selectin–deficient WBCs delivered to HEV by platelets. The percentage of rolling cells that became firmly adherent for a mininum of 30 s (sticking fraction) was determined in the presence or absence of LFA-1 mAb TIB 213. The data shown represent the mean ± SD of seven venules in three animals.
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    Activated human platelets support WBCs homing to subiliac PLN in L-selectin–deficient mice. WBCs were fluorescently labeled in situ using <t>rhodamine</t> 6G and recorded during their passage through single HEV. ( A ) Digitized intravital fluorescence micrograph of a typical HEV in an L-selectin–deficient mouse. Relatively few blood-borne cells ( arrows ) interacted with the vascular wall over a 45-min control period ( 0 min ). ( B ) Intraarterial injection of TRAP-stimulated human platelets over a 10-min period resulted in rolling and accumulation of sticking endogenous WBCs ( 10 min ). ( C ) A significant number of stuck cells subsequently transmigrated into the node ( 45 min ). ( D ) LFA-1 (CD11a) receptor blockade abrogates firm adhesion of L-selectin–deficient WBCs delivered to HEV by platelets. The percentage of rolling cells that became firmly adherent for a mininum of 30 s (sticking fraction) was determined in the presence or absence of LFA-1 mAb TIB 213. The data shown represent the mean ± SD of seven venules in three animals.
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    Activated human platelets support WBCs homing to subiliac PLN in L-selectin–deficient mice. WBCs were fluorescently labeled in situ using <t>rhodamine</t> 6G and recorded during their passage through single HEV. ( A ) Digitized intravital fluorescence micrograph of a typical HEV in an L-selectin–deficient mouse. Relatively few blood-borne cells ( arrows ) interacted with the vascular wall over a 45-min control period ( 0 min ). ( B ) Intraarterial injection of TRAP-stimulated human platelets over a 10-min period resulted in rolling and accumulation of sticking endogenous WBCs ( 10 min ). ( C ) A significant number of stuck cells subsequently transmigrated into the node ( 45 min ). ( D ) LFA-1 (CD11a) receptor blockade abrogates firm adhesion of L-selectin–deficient WBCs delivered to HEV by platelets. The percentage of rolling cells that became firmly adherent for a mininum of 30 s (sticking fraction) was determined in the presence or absence of LFA-1 mAb TIB 213. The data shown represent the mean ± SD of seven venules in three animals.
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    Image Search Results


    Interferogram after removing dc component of 6μm yellow green fluorescent bead (a), Rhodamine 6G solution (b), 4μm red fluorescent bead (c) and its the processed spectrum are (d), (e) and (f). Green curve in spectral graph is the reference

    Journal: Biomedical Optics Express

    Article Title: Depth resolved hyperspectral imaging spectrometer based on structured light illumination and Fourier transform interferometry

    doi: 10.1364/BOE.5.003494

    Figure Lengend Snippet: Interferogram after removing dc component of 6μm yellow green fluorescent bead (a), Rhodamine 6G solution (b), 4μm red fluorescent bead (c) and its the processed spectrum are (d), (e) and (f). Green curve in spectral graph is the reference

    Article Snippet: C F 1 = 473 λ m , C F 2 = 561 λ m , C F 3 = 660 λ m C F = ( C F 1 + C F 2 + C F 3 ) / 3 O P D r e s c a l e d = C F ⋅ O P D c a l c u l a t e d After the initial calibration, the spectrum of the known emission wavelength fluorophores are measured and compared with their reference values. shows the raw interferogram and the corresponding spectrums of 6μm Yellow-Green fluorescent beads (F-8859, Molecular Probe), Rhodamine 6G solution (Sigma-Aldrich), 4μm Red fluorescent beads (F-8858, Molecular Probe).

    Techniques:

    ( a ) normalized Au nanoparticles absorption spectrum (black); rhodamine 6G emission spectra in water solution (red), and MLV (blue); ( b ) relative fluorescence intensity of samples containing Rh6G, MLV with AuNPs measured according to the liposomes suspension with Rh6G; ( c ) variance of maximum Rh6G fluorescence wavelength dependence as a function of the AuNP concentration in the presence of α -PC lipids; ( d ) mean hydrodynamic diameter of samples containing MLV, Rhodamine 6G and AuNPs in a function of nanoparticle amount. At the lowest concentration, only MLVs are visible, but, at higher concentrations, only AuNP are detected by the DLS ( d = 53 nm).

    Journal: Nanomaterials

    Article Title: Plasmonic Nanoparticles Driven Enhanced Light Amplification in a Local 2D and 3D Self-Assembly

    doi: 10.3390/nano8121051

    Figure Lengend Snippet: ( a ) normalized Au nanoparticles absorption spectrum (black); rhodamine 6G emission spectra in water solution (red), and MLV (blue); ( b ) relative fluorescence intensity of samples containing Rh6G, MLV with AuNPs measured according to the liposomes suspension with Rh6G; ( c ) variance of maximum Rh6G fluorescence wavelength dependence as a function of the AuNP concentration in the presence of α -PC lipids; ( d ) mean hydrodynamic diameter of samples containing MLV, Rhodamine 6G and AuNPs in a function of nanoparticle amount. At the lowest concentration, only MLVs are visible, but, at higher concentrations, only AuNP are detected by the DLS ( d = 53 nm).

    Article Snippet: Materials and Methods Rhodamine 6G (Rh6G), dichloromethane (DCM), α -phosphatidylcholine lipids ( α -PC), poly-vinyl alcohol (PVA), tetrahydrofuran (THF, 99%), cetyltrimethylammonium chloride (CTAC) 25% in water, 8-hydroxyquinoline (HQL, 99%), NaBH 4 (99.999%) and NaOH (98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received.

    Techniques: Fluorescence, Concentration Assay

    Human leukocyte interactions with the human microvasculature. A . Representative image of a FITC-conjugated  Ulex europaeus -stained human post-capillary venule from the control SCID-hu mouse.  B . Representative image of rhodamine 6-G labelled human leukocytes rolling and adhering to a  Ulex europaeus -stained human post-capillary venule after 4-hour L-NAME treatment (50 mg/kg b.w., i.p.) in SCID-hu mice.

    Journal: Journal of Inflammation (London, England)

    Article Title: Inhibition of nitric oxide synthesis enhances leukocyte rolling and adhesion in human microvasculature

    doi: 10.1186/1476-9255-9-28

    Figure Lengend Snippet: Human leukocyte interactions with the human microvasculature. A . Representative image of a FITC-conjugated Ulex europaeus -stained human post-capillary venule from the control SCID-hu mouse. B . Representative image of rhodamine 6-G labelled human leukocytes rolling and adhering to a Ulex europaeus -stained human post-capillary venule after 4-hour L-NAME treatment (50 mg/kg b.w., i.p.) in SCID-hu mice.

    Article Snippet: To study the interactions between human leukocytes and human vascular endothelium of human skin graft in SCID mice using fluorescent intravital microscopy, human leukocytes were labelled with rhodamine 6-G (0.025 % final concentration, 5 min; Sigma, St. Louis, MO).

    Techniques: Staining, Mouse Assay

    (A) Linear relationship of Raman intensity with different concentrations of target molecules, including Nile red, rhodamine 6G, and crystal violet, in their individual detection. (B) Multiplexed Raman spectra for triple, double as well as individual detections of Nile red, rhodamine 6G, and crystal violet. (C) The reproducibility of the Raman intensity of Nile red at 591 cm −1 from ten randomly different-batched self-assemblies of 80 nm AuNPs coated with P3 with the concentration of 10 −10 g mL −1 .

    Journal: Nanoscale

    Article Title: Controllable Self-Assembled Plasmonic Vesicle-Based Three-Dimensional SERS Platform for Picomolar Detection of Hydrophobic Contaminants

    doi: 10.1039/c8nr02778a

    Figure Lengend Snippet: (A) Linear relationship of Raman intensity with different concentrations of target molecules, including Nile red, rhodamine 6G, and crystal violet, in their individual detection. (B) Multiplexed Raman spectra for triple, double as well as individual detections of Nile red, rhodamine 6G, and crystal violet. (C) The reproducibility of the Raman intensity of Nile red at 591 cm −1 from ten randomly different-batched self-assemblies of 80 nm AuNPs coated with P3 with the concentration of 10 −10 g mL −1 .

    Article Snippet: Nile red, rhodamine 6G, crystal violet, benzo[a]pyrene (Bap), polychlorinated biphenyls (PCB 7, 77, and 209), tetrahydrofuran (THF), chloroform, acetone, N,N-dimethylformamide (DMF), gold(III) chloride trihydrate (HAuCl4 ), sodium citrate tribasic dihydrate, sodium dodecyl sulfate (SDS), and hydroquinone were purchased from Sigma-Aldrich.

    Techniques: Concentration Assay

    Typical closed cranial window in a mouse. Scale bar = 500 μm ( A ). Representative video images of capturing leukocytes labeled with Rhodamine 6G in the ischemic area. Video images were taken before MCAO ( B ), one hour after MCAO ( C ), and 24 h after

    Journal:

    Article Title: Cannabinoid CB2 receptor activation decreases cerebral infarction in a mouse focal ischemia/reperfusion model

    doi: 10.1038/sj.jcbfm.9600447

    Figure Lengend Snippet: Typical closed cranial window in a mouse. Scale bar = 500 μm ( A ). Representative video images of capturing leukocytes labeled with Rhodamine 6G in the ischemic area. Video images were taken before MCAO ( B ), one hour after MCAO ( C ), and 24 h after

    Article Snippet: Leukocytes were stained in vivo by a bolus injection of 0.05 mL of a 0.01% solution of the fluorescent dye Rhodamine 6G (Sigma Inc.) into the jugular vein.

    Techniques: Labeling

    Rh transport and binding by Cdr1p and Cdr2p. (A) Rh 6G resistance profile of TY310 cells transformed with p425GPD-CDR1 (squares), p425GPD-CDR2 (circles), or p425GPD (triangles) as determined by liquid microdilution assay. Values represent the means ± standard deviations of three independent experiments performed in duplicate. (B) Rh 6G efflux activities of the TY310 transformants mentioned in panel A. Extracellular Rh 6G fluorescence was measured by spectrofluorometry and normalized for the amount of cells present in each sample, as determined by measurement of the OD 600 . Values represent the means ± standard deviations of three independent experiments. (C) Photoaffinity labeling of Cdr1p and Cdr2p with IAARh123. Left panel, total membrane proteins (50 μg) from the transformants described above were incubated with 0.5 μM IAARh123, UV cross-linked, and separated by SDS-PAGE; right panel, total membrane proteins (300 μg) were incubated with 1 μM IAARh123, UV cross-linked, and immunoprecipitated with the anti-Cdrp antibody. The immune complexes were resolved by SDS-PAGE. The gels were dried and exposed to Kodak BioMax MS films with two intensifying screens at −80°C for 4 h (left panel) or 5 days (right panel). The results shown are representative of those of at least three independent experiments. The positions of the molecular mass standards (in kilodaltons) are indicated on the left. CTL, control.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Functional Similarities and Differences between Candida albicans Cdr1p and Cdr2p Transporters

    doi: 10.1128/AAC.47.5.1543-1554.2003

    Figure Lengend Snippet: Rh transport and binding by Cdr1p and Cdr2p. (A) Rh 6G resistance profile of TY310 cells transformed with p425GPD-CDR1 (squares), p425GPD-CDR2 (circles), or p425GPD (triangles) as determined by liquid microdilution assay. Values represent the means ± standard deviations of three independent experiments performed in duplicate. (B) Rh 6G efflux activities of the TY310 transformants mentioned in panel A. Extracellular Rh 6G fluorescence was measured by spectrofluorometry and normalized for the amount of cells present in each sample, as determined by measurement of the OD 600 . Values represent the means ± standard deviations of three independent experiments. (C) Photoaffinity labeling of Cdr1p and Cdr2p with IAARh123. Left panel, total membrane proteins (50 μg) from the transformants described above were incubated with 0.5 μM IAARh123, UV cross-linked, and separated by SDS-PAGE; right panel, total membrane proteins (300 μg) were incubated with 1 μM IAARh123, UV cross-linked, and immunoprecipitated with the anti-Cdrp antibody. The immune complexes were resolved by SDS-PAGE. The gels were dried and exposed to Kodak BioMax MS films with two intensifying screens at −80°C for 4 h (left panel) or 5 days (right panel). The results shown are representative of those of at least three independent experiments. The positions of the molecular mass standards (in kilodaltons) are indicated on the left. CTL, control.

    Article Snippet: Rh 6G (Sigma) was dissolved at 20 mM in ethanol.

    Techniques: Binding Assay, Transformation Assay, Microdilution Assay, Fluorescence, Labeling, Incubation, SDS Page, Immunoprecipitation, Mass Spectrometry, CTL Assay

    In vivo assessment of gingival vascular permeability and leukocyte margination. Rhodamine 6G (R6G; leukocyte labeling) and FITC– dextran (vasculature visualization and tracer molecule) or FITC–LY6G (PMN labeling) were injected intravenously, and intravital microscopy was performed at a controlled body temperature as specified in the Material and methods section. The black arrows indicate the outer side of the vessel wall. (A) The FITC–dextran is retained in the gingival vasculature of a WT mouse. (B) Increased FITC–dextran extravasation in Akita gingiva within the same time period. (C) Rhodamine 6G-positive leukocytes inside gingival postcapillary venules. The FITC–dextran (green) and R6G (red) images were merged offline to obtain this picture. (D) Overlapping images of R6G-positive (red) and LY6G-positive (green) cells indicate that the majority of leukocytes 2 h after tumor necrosis factor α (TNFα) stimulation are polymorphonuclear leukocytes (PMNs; yellow). The white arrows indicate intravascular leukocytes as follows: 1,rolling leukocyte (red); 2, rolling PMN (green); and 3, attached PMN (yellow). Scale bar represents 50 μm; original magnification ×400.

    Journal: Journal of periodontal research

    Article Title: Type 1 diabetes predisposes to enhanced gingival leukocyte margination and macromolecule extravasation in vivo

    doi: 10.1111/j.1600-0765.2010.01295.x

    Figure Lengend Snippet: In vivo assessment of gingival vascular permeability and leukocyte margination. Rhodamine 6G (R6G; leukocyte labeling) and FITC– dextran (vasculature visualization and tracer molecule) or FITC–LY6G (PMN labeling) were injected intravenously, and intravital microscopy was performed at a controlled body temperature as specified in the Material and methods section. The black arrows indicate the outer side of the vessel wall. (A) The FITC–dextran is retained in the gingival vasculature of a WT mouse. (B) Increased FITC–dextran extravasation in Akita gingiva within the same time period. (C) Rhodamine 6G-positive leukocytes inside gingival postcapillary venules. The FITC–dextran (green) and R6G (red) images were merged offline to obtain this picture. (D) Overlapping images of R6G-positive (red) and LY6G-positive (green) cells indicate that the majority of leukocytes 2 h after tumor necrosis factor α (TNFα) stimulation are polymorphonuclear leukocytes (PMNs; yellow). The white arrows indicate intravascular leukocytes as follows: 1,rolling leukocyte (red); 2, rolling PMN (green); and 3, attached PMN (yellow). Scale bar represents 50 μm; original magnification ×400.

    Article Snippet: Rhodamine 6G (R6G; Molecular Probes; Invitrogen, Carlsbad, CA, USA), fluorescein isothiocyanate–dextran (FITC–dextran; molecular weight, 150 kDa), Wright-Giemsa, zymosan A solution (Sigma-Aldrich, St. Louis, MO, USA), FITC-anti-LY6G, anti-PSGL1, anti-CD11a antibodies (BD Biosciences, San Jose, CA, USA), anti-P-selectin, anti-L-selectin antibodies (Santa Cruz, CA, USA) ketamine, xylazine, isoflurane, tumor necrosis factor α (TNFα; Roche Diagnostics), sterile saline solution (0.9% NaCl), phosphate-buffered saline (PBS; Invitrogen Life Technologies, Carlsbad, CA, USA), Bradford protein assay (Bio-Rad, Hercules, CA, USA), 5–0 silk surgical suture (Benco Dental, Pittston, PA, USA), FACScan flow cytometer and Cell Quest computer Software (Becton-Dickinson, Franklin Lakes, NJ, USA), FITC and tetramethylrhodamine isothiocyanate (TRITC) filter sets (Chroma Technology, Rockingham, VT, USA), luminal-enhanced chemiluminescence detection reagent (Cell Signaling Technology, Danvers, MA, USA), Zeiss Axiovert 200 inverted epifluorescent microscope equipped with a Sony DFW-X700 digital video camera, Sonycap (Sony Corporation, Tokyo, Japan) and Olympus MicroSuite software were obtained commercially, as indicated.

    Techniques: In Vivo, Permeability, Labeling, Injection, Intravital Microscopy

    Time course of the remaining amounts of rhodamine 6G (red) and fluorescein (green) in a dialysis cartridge.

    Journal: Molecules

    Article Title: Intracellular Environment-Responsive Stabilization of Polymer Vesicles Formed from Head-Tail Type Polycations Composed of a Polyamidoamine Dendron and Poly(l-lysine)

    doi: 10.3390/molecules181012168

    Figure Lengend Snippet: Time course of the remaining amounts of rhodamine 6G (red) and fluorescein (green) in a dialysis cartridge.

    Article Snippet: Ethylene glycol diglycidyl ether (EGDE), glutathione reduced form (GSH), rhodamine 6G (Rh6G) and fluorescein (Flu) were purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan).

    Techniques:

    Effect of glutathione concentration on the stability of disulfide-bonded nanocapsules ( A ) and the release of rhodamine 6G from nanocapsules ( B ).

    Journal: Molecules

    Article Title: Intracellular Environment-Responsive Stabilization of Polymer Vesicles Formed from Head-Tail Type Polycations Composed of a Polyamidoamine Dendron and Poly(l-lysine)

    doi: 10.3390/molecules181012168

    Figure Lengend Snippet: Effect of glutathione concentration on the stability of disulfide-bonded nanocapsules ( A ) and the release of rhodamine 6G from nanocapsules ( B ).

    Article Snippet: Ethylene glycol diglycidyl ether (EGDE), glutathione reduced form (GSH), rhodamine 6G (Rh6G) and fluorescein (Flu) were purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan).

    Techniques: Concentration Assay

    Single molecule detection of 10 −10 M rhodamine 6G (in agarose) in 4 time intervals of 100 ms each (a–d) including a 3D plot (e) (TIR laser excitation at λ= 514 nm; fluorescence measured at λ ≥ 530 nm; image size: 60 μm ×90 μm).

    Journal: International Journal of Molecular Sciences

    Article Title: Light Dose is a Limiting Factor to Maintain Cell Viability in Fluorescence Microscopy and Single Molecule Detection

    doi: 10.3390/ijms11030956

    Figure Lengend Snippet: Single molecule detection of 10 −10 M rhodamine 6G (in agarose) in 4 time intervals of 100 ms each (a–d) including a 3D plot (e) (TIR laser excitation at λ= 514 nm; fluorescence measured at λ ≥ 530 nm; image size: 60 μm ×90 μm).

    Article Snippet: Cell incubation with the fluorescent membrane marker 6-dodecanoyl-2-dimethylamino naphthalene (laurdan) [ ] was performed in culture medium at a concentration of 8 μM for 1 h. For single molecule experiments highly diluted test samples of 10−10 M rhodamine 6G either dissolved in distilled water or embedded in agarose (1.5% in H2 O; Fluka Chemie, Buchs, Switzerland) were used.

    Techniques: Mass Spectrometry, Fluorescence

    Time dependent fate of Rhodamine-6G labeled CAP nanoparticles (embedded in thermosensitive gel) in HeLa cells after translocation through transwell permeable supports ( n = 3).

    Journal: Journal of biomedical nanotechnology

    Article Title: Thermosensitive Gel Containing Cellulose Acetate Phthalate-Efavirenz Combination Nanoparticles for Prevention of HIV-1 Infection

    doi:

    Figure Lengend Snippet: Time dependent fate of Rhodamine-6G labeled CAP nanoparticles (embedded in thermosensitive gel) in HeLa cells after translocation through transwell permeable supports ( n = 3).

    Article Snippet: Potassium dihydrogen phosphate (HPLC grade), acetonitrile (HPLC grade), dimethyl sulfoxide (DMSO, AR Grade), acetone (AR grade), citric acid (AR grade), trisodium citrate (AR grade) and Rhodamine 6G were purchased from Fischer Scientific Ltd (Pittsburg, PA, USA).

    Techniques: Labeling, Translocation Assay

    Photon burst-size histograms, with and without the Poisson time-filter treatment, in green and red, respectively. ( a ) The native receptor after 2 min in the chamber. ( b ) Rhodamine 6G; the burst size is in units of number of photon counts.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Single-molecule spectroscopy of the ?2 adrenergic receptor: Observation of conformational substates in a membrane protein

    doi: 10.1073/pnas.151239698

    Figure Lengend Snippet: Photon burst-size histograms, with and without the Poisson time-filter treatment, in green and red, respectively. ( a ) The native receptor after 2 min in the chamber. ( b ) Rhodamine 6G; the burst size is in units of number of photon counts.

    Article Snippet: Rhodamine 6G was purchased from Kodak and was dissolved in propylene carbonate from Aldrich.

    Techniques:

    Comparison of Poisson time-filtered photon burst-size distributions from ( a ) native receptor, and ( b ) rhodamine 6G, to the deconvoluted intensity distribution of ( c ) the native receptor and ( d ) rhodamine 6G. See text for details of deconvolution procedure.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Single-molecule spectroscopy of the ?2 adrenergic receptor: Observation of conformational substates in a membrane protein

    doi: 10.1073/pnas.151239698

    Figure Lengend Snippet: Comparison of Poisson time-filtered photon burst-size distributions from ( a ) native receptor, and ( b ) rhodamine 6G, to the deconvoluted intensity distribution of ( c ) the native receptor and ( d ) rhodamine 6G. See text for details of deconvolution procedure.

    Article Snippet: Rhodamine 6G was purchased from Kodak and was dissolved in propylene carbonate from Aldrich.

    Techniques:

    Manipulation of F 1  rotor motion by optical and chemical inputs . Sequential images of a rotating beads before and after a pulse (10 sec) of high intensity white light illumination (white bar) in the presence (A) or absence (B) of rhodamine 6G. (C) Rotating beads in the presence of rhodamine 6G, but without light pulse.

    Journal: Journal of Nanobiotechnology

    Article Title: Two-stimuli manipulation of a biological motor

    doi: 10.1186/1477-3155-7-3

    Figure Lengend Snippet: Manipulation of F 1 rotor motion by optical and chemical inputs . Sequential images of a rotating beads before and after a pulse (10 sec) of high intensity white light illumination (white bar) in the presence (A) or absence (B) of rhodamine 6G. (C) Rotating beads in the presence of rhodamine 6G, but without light pulse.

    Article Snippet: Rhodamine 6G at higher concentration is believed to bind F1 -ATPase at least at two binding sites [ - ].

    Techniques: Size-exclusion Chromatography

    ( a ) Transmission electron microscopy (TEM) image of 30 nm colloidal Au nanoparticles (NPs); ( b ) surface-enhanced Raman scattering (SERS) spectra of 10 −3 M rhodamine 6G (R6G) using colloidal Au NPs with different sizes (10, 20, 30, 40, and 50 nm); ( c ) SERS spectra of R6G with concentrations from 10 −17 to 10 −2 M (bottom to top, concentration successively increasing by a factor of 10) using 30 nm colloidal Au NPs. The intensity is multiplied five times for 10 −11 to 10 −7 M R6G and 10 times for 10 −17 to 10 −12 M R6G; ( d ) Linear relationship between log I of the band peak at 1361 cm −1 and log C based on the SERS data of R6G in ( c ).

    Journal: Sensors (Basel, Switzerland)

    Article Title: Quantitative and Sensitive Detection of Chloramphenicol by Surface-Enhanced Raman Scattering

    doi: 10.3390/s17122962

    Figure Lengend Snippet: ( a ) Transmission electron microscopy (TEM) image of 30 nm colloidal Au nanoparticles (NPs); ( b ) surface-enhanced Raman scattering (SERS) spectra of 10 −3 M rhodamine 6G (R6G) using colloidal Au NPs with different sizes (10, 20, 30, 40, and 50 nm); ( c ) SERS spectra of R6G with concentrations from 10 −17 to 10 −2 M (bottom to top, concentration successively increasing by a factor of 10) using 30 nm colloidal Au NPs. The intensity is multiplied five times for 10 −11 to 10 −7 M R6G and 10 times for 10 −17 to 10 −12 M R6G; ( d ) Linear relationship between log I of the band peak at 1361 cm −1 and log C based on the SERS data of R6G in ( c ).

    Article Snippet: Materials and Instruments Chloroauric acid (HAuCl4 ), sodium citrate, and AgNO3 were purchased from Shanghai Aladdin Regent Co. Ltd. (Shanghai, China), rhodamine 6G (R6G) was purchased from J & K Scientific LTD.

    Techniques: Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Concentration Assay

    Activated human platelets support WBCs homing to subiliac PLN in L-selectin–deficient mice. WBCs were fluorescently labeled in situ using rhodamine 6G and recorded during their passage through single HEV. ( A ) Digitized intravital fluorescence micrograph of a typical HEV in an L-selectin–deficient mouse. Relatively few blood-borne cells ( arrows ) interacted with the vascular wall over a 45-min control period ( 0 min ). ( B ) Intraarterial injection of TRAP-stimulated human platelets over a 10-min period resulted in rolling and accumulation of sticking endogenous WBCs ( 10 min ). ( C ) A significant number of stuck cells subsequently transmigrated into the node ( 45 min ). ( D ) LFA-1 (CD11a) receptor blockade abrogates firm adhesion of L-selectin–deficient WBCs delivered to HEV by platelets. The percentage of rolling cells that became firmly adherent for a mininum of 30 s (sticking fraction) was determined in the presence or absence of LFA-1 mAb TIB 213. The data shown represent the mean ± SD of seven venules in three animals.

    Journal: The Journal of Experimental Medicine

    Article Title: Circulating Activated Platelets Reconstitute Lymphocyte Homing and Immunity in L-selectin-Deficient Mice

    doi:

    Figure Lengend Snippet: Activated human platelets support WBCs homing to subiliac PLN in L-selectin–deficient mice. WBCs were fluorescently labeled in situ using rhodamine 6G and recorded during their passage through single HEV. ( A ) Digitized intravital fluorescence micrograph of a typical HEV in an L-selectin–deficient mouse. Relatively few blood-borne cells ( arrows ) interacted with the vascular wall over a 45-min control period ( 0 min ). ( B ) Intraarterial injection of TRAP-stimulated human platelets over a 10-min period resulted in rolling and accumulation of sticking endogenous WBCs ( 10 min ). ( C ) A significant number of stuck cells subsequently transmigrated into the node ( 45 min ). ( D ) LFA-1 (CD11a) receptor blockade abrogates firm adhesion of L-selectin–deficient WBCs delivered to HEV by platelets. The percentage of rolling cells that became firmly adherent for a mininum of 30 s (sticking fraction) was determined in the presence or absence of LFA-1 mAb TIB 213. The data shown represent the mean ± SD of seven venules in three animals.

    Article Snippet: For microscopic visualization of endogenous white blood cell (WBC) interactions with vascular endothelium, a bolus injection of saline (10 ml/kg body weight) containing 1 mg/ml of the nuclear dye Rhodamine 6G (Molecular Probes, Inc., Eugene, OR) was given intravenously.

    Techniques: Mouse Assay, Labeling, In Situ, Fluorescence, Injection

    Effect of circulating, activated platelets on WBCs rolling in  PLN–HEV of mice deficient in L-selectin. WBCs (mononuclear cells and  granulocytes) were fluorescently labeled in situ with rhodamine 6G, visualized in HEVs of PLN of wild-type ( closed bars ) and L-selectin–deficient  ( open bars ) mice by fluorescence intravital microscopy, and rolling fractions quantitated as previously described (  10 ). The effect of mAbs and  platelet injections on the WBC rolling fraction was determined in identical fields of view. The L-selectin blocking mAb Mel-14 was tested in 10  venules of 4 wild-type animals. The ability of platelets to reconstitute rolling in mutant animals was examined by injecting TRAP-activated or resting, human platelets (12 venules in 5 animals). The ability of the human  P-selectin mAb WAPS 12.2 and murine LFA-1 mAb TIB 213 to inhibit  WBC rolling was also evaluated (four venules in three animals each). Data  are shown as mean ± SD.

    Journal: The Journal of Experimental Medicine

    Article Title: Circulating Activated Platelets Reconstitute Lymphocyte Homing and Immunity in L-selectin-Deficient Mice

    doi:

    Figure Lengend Snippet: Effect of circulating, activated platelets on WBCs rolling in PLN–HEV of mice deficient in L-selectin. WBCs (mononuclear cells and granulocytes) were fluorescently labeled in situ with rhodamine 6G, visualized in HEVs of PLN of wild-type ( closed bars ) and L-selectin–deficient ( open bars ) mice by fluorescence intravital microscopy, and rolling fractions quantitated as previously described ( 10 ). The effect of mAbs and platelet injections on the WBC rolling fraction was determined in identical fields of view. The L-selectin blocking mAb Mel-14 was tested in 10 venules of 4 wild-type animals. The ability of platelets to reconstitute rolling in mutant animals was examined by injecting TRAP-activated or resting, human platelets (12 venules in 5 animals). The ability of the human P-selectin mAb WAPS 12.2 and murine LFA-1 mAb TIB 213 to inhibit WBC rolling was also evaluated (four venules in three animals each). Data are shown as mean ± SD.

    Article Snippet: For microscopic visualization of endogenous white blood cell (WBC) interactions with vascular endothelium, a bolus injection of saline (10 ml/kg body weight) containing 1 mg/ml of the nuclear dye Rhodamine 6G (Molecular Probes, Inc., Eugene, OR) was given intravenously.

    Techniques: Mouse Assay, Labeling, In Situ, Fluorescence, Intravital Microscopy, Blocking Assay, Mutagenesis

    Lymphocyte delivery to PLN of L-selectin–deficient mice does not lead to platelet accumulation in HEV. The micrographs show a segment of the same HEV as in Fig. 2 (blood flow from right to left) 10 min after injection of activated human platelets. ( A ) A rolling aggregate of bright 2′7′-bis-(2-carboxyethyl)-5(and 6) carboxyfluorescein– labeled platelets with a fainter rhodamine 6G labeled WBC in their midst can be seen ( arrow ). ( B ) 2 s later, the platelet–WBC aggregate has arrested firmly. Labeled platelets are initially distributed randomly on the surface of the stuck cell. ( C ) Within 5 s after the WBC had become stuck, platelets roll slowly downstream across the body of the adherent cell and eventually detach. ( D ) 30 s later, all platelets have detached and returned to the blood stream, whereas the WBC remains stuck at the HEV surface.

    Journal: The Journal of Experimental Medicine

    Article Title: Circulating Activated Platelets Reconstitute Lymphocyte Homing and Immunity in L-selectin-Deficient Mice

    doi:

    Figure Lengend Snippet: Lymphocyte delivery to PLN of L-selectin–deficient mice does not lead to platelet accumulation in HEV. The micrographs show a segment of the same HEV as in Fig. 2 (blood flow from right to left) 10 min after injection of activated human platelets. ( A ) A rolling aggregate of bright 2′7′-bis-(2-carboxyethyl)-5(and 6) carboxyfluorescein– labeled platelets with a fainter rhodamine 6G labeled WBC in their midst can be seen ( arrow ). ( B ) 2 s later, the platelet–WBC aggregate has arrested firmly. Labeled platelets are initially distributed randomly on the surface of the stuck cell. ( C ) Within 5 s after the WBC had become stuck, platelets roll slowly downstream across the body of the adherent cell and eventually detach. ( D ) 30 s later, all platelets have detached and returned to the blood stream, whereas the WBC remains stuck at the HEV surface.

    Article Snippet: For microscopic visualization of endogenous white blood cell (WBC) interactions with vascular endothelium, a bolus injection of saline (10 ml/kg body weight) containing 1 mg/ml of the nuclear dye Rhodamine 6G (Molecular Probes, Inc., Eugene, OR) was given intravenously.

    Techniques: Mouse Assay, Flow Cytometry, Injection, Labeling