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  • 94
    New England Biolabs m13mp18 rfi dsdna
    M13mp18 Rfi Dsdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher φx174 rfi dna
    φx174 Rfi Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rfi  (Zoetis)
    88
    Zoetis rfi
    Rfi, supplied by Zoetis, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rfi  (Kiyatec)
    90
    Kiyatec rfi
    Rfi, supplied by Kiyatec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    SAS institute rfi
    Rfi, supplied by SAS institute, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biotechnology Information rfi
    Determination of potential key operational taxonomic units <t>(OTUs)</t> at 16 days post-hatch for (A) residual feed intake <t>(RFI)</t> and (B) performance traits [total feed intake (TFI) and total body weight gain (TBWG) between 9 and 14 days post-hatch] in chickens ranked on RFI and fed either ad libitum or restrictively. Covariations between the relative abundances of bacterial OTUs in the feces and RFI, and performance traits using sparse partial least squares regression. The OTUs were included in the analysis if they occurred in at least half of the chickens. The network is displayed graphically as nodes (OTUs and performance traits) and edges (biological relationships between the nodes), with the edge color intensity indicating the level of the association: red = positive, and green = negative. Only the strongest pairwise associations were displayed, with score threshold depending of the respective association. The association scores are indicated under each edge. The cut-off threshold for the pairwise associations scores were 0.20 (positive and negative) in (A) , and 0.30 (positive and negative) in (B) .
    Rfi, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    SAS institute rfi line
    Determination of potential key operational taxonomic units <t>(OTUs)</t> at 16 days post-hatch for (A) residual feed intake <t>(RFI)</t> and (B) performance traits [total feed intake (TFI) and total body weight gain (TBWG) between 9 and 14 days post-hatch] in chickens ranked on RFI and fed either ad libitum or restrictively. Covariations between the relative abundances of bacterial OTUs in the feces and RFI, and performance traits using sparse partial least squares regression. The OTUs were included in the analysis if they occurred in at least half of the chickens. The network is displayed graphically as nodes (OTUs and performance traits) and edges (biological relationships between the nodes), with the edge color intensity indicating the level of the association: red = positive, and green = negative. Only the strongest pairwise associations were displayed, with score threshold depending of the respective association. The association scores are indicated under each edge. The cut-off threshold for the pairwise associations scores were 0.20 (positive and negative) in (A) , and 0.30 (positive and negative) in (B) .
    Rfi Line, supplied by SAS institute, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher m13mp18 rfi
    Determination of potential key operational taxonomic units <t>(OTUs)</t> at 16 days post-hatch for (A) residual feed intake <t>(RFI)</t> and (B) performance traits [total feed intake (TFI) and total body weight gain (TBWG) between 9 and 14 days post-hatch] in chickens ranked on RFI and fed either ad libitum or restrictively. Covariations between the relative abundances of bacterial OTUs in the feces and RFI, and performance traits using sparse partial least squares regression. The OTUs were included in the analysis if they occurred in at least half of the chickens. The network is displayed graphically as nodes (OTUs and performance traits) and edges (biological relationships between the nodes), with the edge color intensity indicating the level of the association: red = positive, and green = negative. Only the strongest pairwise associations were displayed, with score threshold depending of the respective association. The association scores are indicated under each edge. The cut-off threshold for the pairwise associations scores were 0.20 (positive and negative) in (A) , and 0.30 (positive and negative) in (B) .
    M13mp18 Rfi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher i rfi dna
    MoMLV PIC is compacted by BAF. (A) Sedimentation properties of initial (panel 1), salt-stripped (panel 2), BAF-reconstituted (panel 3), HMGA1-reconstituted (panels 4 and 5), and HMGB1-reconstituted (panels 6 and 7) PICs. The MoMLV PIC fraction was salt stripped with 750 mM KCl and then incubated with BAF, HMGA1, or HMGB1 protein in the presence of 400 mM KCl (panels 1, 2, 3, 4, and 6) or 150 mM KCl (panels 5 and 7). After gel filtration, each reconstituted PIC fraction was layered on a 15 to 30% sucrose gradient and centrifuged at 30,000 rpm for 1 h with a Beckman rotor. The gradient was fractionated from the top to bottom into 22 fractions, and viral <t>DNA</t> in each fraction was detected by Southern blotting. (B) Intermolecular integration activities of initial, salt-stripped, and reconstituted PICs. Salt-stripped PICs were incubated with BAF (lane 3), HMGA1 (lanes 4 and 6), or HMGB1 (lanes 5 and 7) in the presence of 400 mM KCl (lanes 1 to 5) or 150 mM KCl (lanes 6 and 7) and then added to the integration reaction mixture containing φX174 <t>RFI</t> DNA as the target DNA to check integration activities. After incubation, DNA products from the reaction were digested with Bam HI and detected by Southern blotting with a 32 ).
    I Rfi Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    New England Biolabs phix174 rfi plasmid
    Substrate specificity of Endo IV. The activity of Endo IV (1, 0.5, 0.2, 0.1 or 0.05 μg/ml) was assayed with phiX174 <t>RFI</t> circular <t>dsDNA</t> ( A ), phiX174 RFI linear dsDNA ( B ), phiX174 virion circular ssDNA ( C ), linear ssRNA used for the in vitro synthesis of the GST-Endo IV fusion protein ( D ), heat-denatured T4 genomic dsDNA containing gluc-dHMC ( E ), or heat-denatured dC-substituted T4 (T4dC) genomic dsDNA ( F ) as the substrate (5 μg/ml). The reaction products were separated by electrophoresis on a 1.0% agarose gel and stained with SYBR Gold. Lanes M and (–) contain a 1 kb DNA ladder and a reaction mixture incubated in the absence of the enzyme, respectively.
    Phix174 Rfi Plasmid, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare inra low rfi line
    Substrate specificity of Endo IV. The activity of Endo IV (1, 0.5, 0.2, 0.1 or 0.05 μg/ml) was assayed with phiX174 <t>RFI</t> circular <t>dsDNA</t> ( A ), phiX174 RFI linear dsDNA ( B ), phiX174 virion circular ssDNA ( C ), linear ssRNA used for the in vitro synthesis of the GST-Endo IV fusion protein ( D ), heat-denatured T4 genomic dsDNA containing gluc-dHMC ( E ), or heat-denatured dC-substituted T4 (T4dC) genomic dsDNA ( F ) as the substrate (5 μg/ml). The reaction products were separated by electrophoresis on a 1.0% agarose gel and stained with SYBR Gold. Lanes M and (–) contain a 1 kb DNA ladder and a reaction mixture incubated in the absence of the enzyme, respectively.
    Inra Low Rfi Line, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Double Helix øx174 dna rfi
    Substrate specificity of Endo IV. The activity of Endo IV (1, 0.5, 0.2, 0.1 or 0.05 μg/ml) was assayed with phiX174 <t>RFI</t> circular <t>dsDNA</t> ( A ), phiX174 RFI linear dsDNA ( B ), phiX174 virion circular ssDNA ( C ), linear ssRNA used for the in vitro synthesis of the GST-Endo IV fusion protein ( D ), heat-denatured T4 genomic dsDNA containing gluc-dHMC ( E ), or heat-denatured dC-substituted T4 (T4dC) genomic dsDNA ( F ) as the substrate (5 μg/ml). The reaction products were separated by electrophoresis on a 1.0% agarose gel and stained with SYBR Gold. Lanes M and (–) contain a 1 kb DNA ladder and a reaction mixture incubated in the absence of the enzyme, respectively.
    øx174 Dna Rfi, supplied by Double Helix, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher φx174 rfi plasmid dna
    Substrate specificity of Endo IV. The activity of Endo IV (1, 0.5, 0.2, 0.1 or 0.05 μg/ml) was assayed with phiX174 <t>RFI</t> circular <t>dsDNA</t> ( A ), phiX174 RFI linear dsDNA ( B ), phiX174 virion circular ssDNA ( C ), linear ssRNA used for the in vitro synthesis of the GST-Endo IV fusion protein ( D ), heat-denatured T4 genomic dsDNA containing gluc-dHMC ( E ), or heat-denatured dC-substituted T4 (T4dC) genomic dsDNA ( F ) as the substrate (5 μg/ml). The reaction products were separated by electrophoresis on a 1.0% agarose gel and stained with SYBR Gold. Lanes M and (–) contain a 1 kb DNA ladder and a reaction mixture incubated in the absence of the enzyme, respectively.
    φx174 Rfi Plasmid Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    New England Biolabs double strand circular φx174 rfi dna
    Substrate specificity of Endo IV. The activity of Endo IV (1, 0.5, 0.2, 0.1 or 0.05 μg/ml) was assayed with phiX174 <t>RFI</t> circular <t>dsDNA</t> ( A ), phiX174 RFI linear dsDNA ( B ), phiX174 virion circular ssDNA ( C ), linear ssRNA used for the in vitro synthesis of the GST-Endo IV fusion protein ( D ), heat-denatured T4 genomic dsDNA containing gluc-dHMC ( E ), or heat-denatured dC-substituted T4 (T4dC) genomic dsDNA ( F ) as the substrate (5 μg/ml). The reaction products were separated by electrophoresis on a 1.0% agarose gel and stained with SYBR Gold. Lanes M and (–) contain a 1 kb DNA ladder and a reaction mixture incubated in the absence of the enzyme, respectively.
    Double Strand Circular φx174 Rfi Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher φx174 rfi supercoiled circular duplex dna
    Substrate specificity of Endo IV. The activity of Endo IV (1, 0.5, 0.2, 0.1 or 0.05 μg/ml) was assayed with phiX174 <t>RFI</t> circular <t>dsDNA</t> ( A ), phiX174 RFI linear dsDNA ( B ), phiX174 virion circular ssDNA ( C ), linear ssRNA used for the in vitro synthesis of the GST-Endo IV fusion protein ( D ), heat-denatured T4 genomic dsDNA containing gluc-dHMC ( E ), or heat-denatured dC-substituted T4 (T4dC) genomic dsDNA ( F ) as the substrate (5 μg/ml). The reaction products were separated by electrophoresis on a 1.0% agarose gel and stained with SYBR Gold. Lanes M and (–) contain a 1 kb DNA ladder and a reaction mixture incubated in the absence of the enzyme, respectively.
    φx174 Rfi Supercoiled Circular Duplex Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Determination of potential key operational taxonomic units (OTUs) at 16 days post-hatch for (A) residual feed intake (RFI) and (B) performance traits [total feed intake (TFI) and total body weight gain (TBWG) between 9 and 14 days post-hatch] in chickens ranked on RFI and fed either ad libitum or restrictively. Covariations between the relative abundances of bacterial OTUs in the feces and RFI, and performance traits using sparse partial least squares regression. The OTUs were included in the analysis if they occurred in at least half of the chickens. The network is displayed graphically as nodes (OTUs and performance traits) and edges (biological relationships between the nodes), with the edge color intensity indicating the level of the association: red = positive, and green = negative. Only the strongest pairwise associations were displayed, with score threshold depending of the respective association. The association scores are indicated under each edge. The cut-off threshold for the pairwise associations scores were 0.20 (positive and negative) in (A) , and 0.30 (positive and negative) in (B) .

    Journal: Frontiers in Microbiology

    Article Title: Feed Restriction Modulates the Fecal Microbiota Composition, Nutrient Retention, and Feed Efficiency in Chickens Divergent in Residual Feed Intake

    doi: 10.3389/fmicb.2018.02698

    Figure Lengend Snippet: Determination of potential key operational taxonomic units (OTUs) at 16 days post-hatch for (A) residual feed intake (RFI) and (B) performance traits [total feed intake (TFI) and total body weight gain (TBWG) between 9 and 14 days post-hatch] in chickens ranked on RFI and fed either ad libitum or restrictively. Covariations between the relative abundances of bacterial OTUs in the feces and RFI, and performance traits using sparse partial least squares regression. The OTUs were included in the analysis if they occurred in at least half of the chickens. The network is displayed graphically as nodes (OTUs and performance traits) and edges (biological relationships between the nodes), with the edge color intensity indicating the level of the association: red = positive, and green = negative. Only the strongest pairwise associations were displayed, with score threshold depending of the respective association. The association scores are indicated under each edge. The cut-off threshold for the pairwise associations scores were 0.20 (positive and negative) in (A) , and 0.30 (positive and negative) in (B) .

    Article Snippet: Representative sequences of the most abundant OTUs that were differently affected by time point, restrictive feeding, and RFI were additionally classified against the National Center for Biotechnology Information ( NCBI ) nucleotide database using Blastn for taxonomic classification ( https://blast.ncbi.nlm.nih.gov/ ).

    Techniques:

    Determination of potential key operational taxonomic units (OTUs) at 29 days post-hatch for (A) residual feed intake (RFI), (B) phosphorus retention (Pret), and (C) performance traits (total feed intake (TFI) and total body weight gain (TBWG) between 9 and 30 days post-hatch) in chickens ranked on RFI and fed either ad libitum or restrictively. Covariations between the relative abundances of bacterial OTUs in the feces and RFI, Pret, and performance traits using sparse partial least squares regression. The OTUs were included in the analysis if they occurred in at least half of the chickens. The network is displayed graphically as nodes (OTUs and Pret, and performance traits) and edges (biological relationships between the nodes), with the edge color intensity indicating the level of the association: red = positive, and green = negative. Only the strongest pairwise associations were displayed, with score threshold depending of the respective association. The association scores are indicated under each edge. The cut-off threshold for the pairwise associations scores were 0.20 (positive and negative) in (A) , and 0.30 (positive and negative) in (B,C) .

    Journal: Frontiers in Microbiology

    Article Title: Feed Restriction Modulates the Fecal Microbiota Composition, Nutrient Retention, and Feed Efficiency in Chickens Divergent in Residual Feed Intake

    doi: 10.3389/fmicb.2018.02698

    Figure Lengend Snippet: Determination of potential key operational taxonomic units (OTUs) at 29 days post-hatch for (A) residual feed intake (RFI), (B) phosphorus retention (Pret), and (C) performance traits (total feed intake (TFI) and total body weight gain (TBWG) between 9 and 30 days post-hatch) in chickens ranked on RFI and fed either ad libitum or restrictively. Covariations between the relative abundances of bacterial OTUs in the feces and RFI, Pret, and performance traits using sparse partial least squares regression. The OTUs were included in the analysis if they occurred in at least half of the chickens. The network is displayed graphically as nodes (OTUs and Pret, and performance traits) and edges (biological relationships between the nodes), with the edge color intensity indicating the level of the association: red = positive, and green = negative. Only the strongest pairwise associations were displayed, with score threshold depending of the respective association. The association scores are indicated under each edge. The cut-off threshold for the pairwise associations scores were 0.20 (positive and negative) in (A) , and 0.30 (positive and negative) in (B,C) .

    Article Snippet: Representative sequences of the most abundant OTUs that were differently affected by time point, restrictive feeding, and RFI were additionally classified against the National Center for Biotechnology Information ( NCBI ) nucleotide database using Blastn for taxonomic classification ( https://blast.ncbi.nlm.nih.gov/ ).

    Techniques:

    MoMLV PIC is compacted by BAF. (A) Sedimentation properties of initial (panel 1), salt-stripped (panel 2), BAF-reconstituted (panel 3), HMGA1-reconstituted (panels 4 and 5), and HMGB1-reconstituted (panels 6 and 7) PICs. The MoMLV PIC fraction was salt stripped with 750 mM KCl and then incubated with BAF, HMGA1, or HMGB1 protein in the presence of 400 mM KCl (panels 1, 2, 3, 4, and 6) or 150 mM KCl (panels 5 and 7). After gel filtration, each reconstituted PIC fraction was layered on a 15 to 30% sucrose gradient and centrifuged at 30,000 rpm for 1 h with a Beckman rotor. The gradient was fractionated from the top to bottom into 22 fractions, and viral DNA in each fraction was detected by Southern blotting. (B) Intermolecular integration activities of initial, salt-stripped, and reconstituted PICs. Salt-stripped PICs were incubated with BAF (lane 3), HMGA1 (lanes 4 and 6), or HMGB1 (lanes 5 and 7) in the presence of 400 mM KCl (lanes 1 to 5) or 150 mM KCl (lanes 6 and 7) and then added to the integration reaction mixture containing φX174 RFI DNA as the target DNA to check integration activities. After incubation, DNA products from the reaction were digested with Bam HI and detected by Southern blotting with a 32 ).

    Journal: Journal of Virology

    Article Title: Regulatory Mechanisms by Which Barrier-to-Autointegration Factor Blocks Autointegration and Stimulates Intermolecular Integration of Moloney Murine Leukemia Virus Preintegration Complexes

    doi: 10.1128/JVI.76.23.12376-12380.2002

    Figure Lengend Snippet: MoMLV PIC is compacted by BAF. (A) Sedimentation properties of initial (panel 1), salt-stripped (panel 2), BAF-reconstituted (panel 3), HMGA1-reconstituted (panels 4 and 5), and HMGB1-reconstituted (panels 6 and 7) PICs. The MoMLV PIC fraction was salt stripped with 750 mM KCl and then incubated with BAF, HMGA1, or HMGB1 protein in the presence of 400 mM KCl (panels 1, 2, 3, 4, and 6) or 150 mM KCl (panels 5 and 7). After gel filtration, each reconstituted PIC fraction was layered on a 15 to 30% sucrose gradient and centrifuged at 30,000 rpm for 1 h with a Beckman rotor. The gradient was fractionated from the top to bottom into 22 fractions, and viral DNA in each fraction was detected by Southern blotting. (B) Intermolecular integration activities of initial, salt-stripped, and reconstituted PICs. Salt-stripped PICs were incubated with BAF (lane 3), HMGA1 (lanes 4 and 6), or HMGB1 (lanes 5 and 7) in the presence of 400 mM KCl (lanes 1 to 5) or 150 mM KCl (lanes 6 and 7) and then added to the integration reaction mixture containing φX174 RFI DNA as the target DNA to check integration activities. After incubation, DNA products from the reaction were digested with Bam HI and detected by Southern blotting with a 32 ).

    Article Snippet: Integration activities of reconstituted PICs were evaluated by the previously described integration activity assay with φX174 replicative form I (RFI) DNA (Gibco BRL) as the target DNA ( ).

    Techniques: Sedimentation, Incubation, Filtration, Southern Blot

    Substrate specificity of Endo IV. The activity of Endo IV (1, 0.5, 0.2, 0.1 or 0.05 μg/ml) was assayed with phiX174 RFI circular dsDNA ( A ), phiX174 RFI linear dsDNA ( B ), phiX174 virion circular ssDNA ( C ), linear ssRNA used for the in vitro synthesis of the GST-Endo IV fusion protein ( D ), heat-denatured T4 genomic dsDNA containing gluc-dHMC ( E ), or heat-denatured dC-substituted T4 (T4dC) genomic dsDNA ( F ) as the substrate (5 μg/ml). The reaction products were separated by electrophoresis on a 1.0% agarose gel and stained with SYBR Gold. Lanes M and (–) contain a 1 kb DNA ladder and a reaction mixture incubated in the absence of the enzyme, respectively.

    Journal: Nucleic Acids Research

    Article Title: Biochemical analysis of the substrate specificity and sequence preference of endonuclease IV from bacteriophage T4, a dC-specific endonuclease implicated in restriction of dC-substituted T4 DNA synthesis

    doi: 10.1093/nar/gkl553

    Figure Lengend Snippet: Substrate specificity of Endo IV. The activity of Endo IV (1, 0.5, 0.2, 0.1 or 0.05 μg/ml) was assayed with phiX174 RFI circular dsDNA ( A ), phiX174 RFI linear dsDNA ( B ), phiX174 virion circular ssDNA ( C ), linear ssRNA used for the in vitro synthesis of the GST-Endo IV fusion protein ( D ), heat-denatured T4 genomic dsDNA containing gluc-dHMC ( E ), or heat-denatured dC-substituted T4 (T4dC) genomic dsDNA ( F ) as the substrate (5 μg/ml). The reaction products were separated by electrophoresis on a 1.0% agarose gel and stained with SYBR Gold. Lanes M and (–) contain a 1 kb DNA ladder and a reaction mixture incubated in the absence of the enzyme, respectively.

    Article Snippet: Purified Endo IV was diluted in a solution containing 10 mM Tris–HCl (pH 8.0), 1 mM DTT and BSA (0.1 mg/ml) and was included in the reaction mixture in a volume of 1 μl. phiX174 replicative form I (RFI) circular dsDNA (New England BioLabs), phiX174 RFI linear dsDNA prepared by PstI digestion, phiX174 virion circular ssDNA (New England BioLabs), linear ssRNA used for synthesis of the GST-Endo IV fusion protein, and heat-denatured T4 or T4dC genomic dsDNA were used as substrates at a final concentration of 5 μg/ml.

    Techniques: Activity Assay, In Vitro, Electrophoresis, Agarose Gel Electrophoresis, Staining, Incubation