revertaid h minus first strand cdna synthesis kit Search Results


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    Thermo Fisher revertaid h minus first strand cdna synthesis kit
    PCR amplification and expression of K. pneumoniae MGH 78578 <t>yggG</t> ORF. A 696 bp PCR product was amplified from genomic DNA (lane 1) or <t>cDNA</t> (lane 2). The PCR product from lane 1 was purified and cloned into a pET-14b vector for protein expression “–”lanes indicate no-template controls. Lane 3: Purified K. pneumoniae YggG protein on 12% SDS-PAGE. M1: GeneRuler 1 kbp DNA ladder (Fermentas); M2: BenchMark ™ protein ladder.
    Revertaid H Minus First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 17242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid h minus first strand cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 17242 article reviews
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    revertaid h minus first strand cdna synthesis kit - by Bioz Stars, 2020-03
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    PCR amplification and expression of K. pneumoniae MGH 78578 yggG ORF. A 696 bp PCR product was amplified from genomic DNA (lane 1) or cDNA (lane 2). The PCR product from lane 1 was purified and cloned into a pET-14b vector for protein expression “–”lanes indicate no-template controls. Lane 3: Purified K. pneumoniae YggG protein on 12% SDS-PAGE. M1: GeneRuler 1 kbp DNA ladder (Fermentas); M2: BenchMark ™ protein ladder.

    Journal: International Journal of Molecular Sciences

    Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease

    doi: 10.3390/ijms12074441

    Figure Lengend Snippet: PCR amplification and expression of K. pneumoniae MGH 78578 yggG ORF. A 696 bp PCR product was amplified from genomic DNA (lane 1) or cDNA (lane 2). The PCR product from lane 1 was purified and cloned into a pET-14b vector for protein expression “–”lanes indicate no-template controls. Lane 3: Purified K. pneumoniae YggG protein on 12% SDS-PAGE. M1: GeneRuler 1 kbp DNA ladder (Fermentas); M2: BenchMark ™ protein ladder.

    Article Snippet: Reverse Transcription PCR of yggG Gene RevertAid H Minus first strand cDNA synthesis kit (Fermentas) was used to synthesize the cDNA from the extracted total RNA.

    Techniques: Polymerase Chain Reaction, Amplification, Expressing, Purification, Clone Assay, Positron Emission Tomography, Plasmid Preparation, SDS Page