reverse transcription rt - polymerase chain reaction pcr assay Search Results


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  • 99
    Thermo Fisher real time reverse transcription polymerase chain reaction rt pcr taqman assays
    Constitutive activation of β-catenin induces p53-activation and apoptosis in small intestine explants from ApcZfp148 mice A. - B. <t>Taqman</t> <t>RT-PCR</t> assessment of mRNA levels of three β-catenin target genes A. and four p53-target genes B. in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM of the GSK3β-inhibitor GSK3IX or DMSO. The explants in A. were treated for 6 hours and those in B. for 20 hours ( n = 6). C. Western blots of phosphorylated and total p53, the p53 targets p21 and Bax, and the apoptosis marker cleaved Parp1 (89-kD fragment) in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM GSK3IX or DMSO for 20 hours. Asterisk indicates uncleaved Parp1 (116-kD fragment). β-Actin was used as loading control. Data are represented as mean ± SEM. * P
    Real Time Reverse Transcription Polymerase Chain Reaction Rt Pcr Taqman Assays, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche ultrasensitive reverse transcription polymerase chain reaction rt pcr assay
    Constitutive activation of β-catenin induces p53-activation and apoptosis in small intestine explants from ApcZfp148 mice A. - B. <t>Taqman</t> <t>RT-PCR</t> assessment of mRNA levels of three β-catenin target genes A. and four p53-target genes B. in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM of the GSK3β-inhibitor GSK3IX or DMSO. The explants in A. were treated for 6 hours and those in B. for 20 hours ( n = 6). C. Western blots of phosphorylated and total p53, the p53 targets p21 and Bax, and the apoptosis marker cleaved Parp1 (89-kD fragment) in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM GSK3IX or DMSO for 20 hours. Asterisk indicates uncleaved Parp1 (116-kD fragment). β-Actin was used as loading control. Data are represented as mean ± SEM. * P
    Ultrasensitive Reverse Transcription Polymerase Chain Reaction Rt Pcr Assay, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abbott Laboratories in vitro reverse transcription polymerase chain reaction rt pcr assay
    Constitutive activation of β-catenin induces p53-activation and apoptosis in small intestine explants from ApcZfp148 mice A. - B. <t>Taqman</t> <t>RT-PCR</t> assessment of mRNA levels of three β-catenin target genes A. and four p53-target genes B. in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM of the GSK3β-inhibitor GSK3IX or DMSO. The explants in A. were treated for 6 hours and those in B. for 20 hours ( n = 6). C. Western blots of phosphorylated and total p53, the p53 targets p21 and Bax, and the apoptosis marker cleaved Parp1 (89-kD fragment) in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM GSK3IX or DMSO for 20 hours. Asterisk indicates uncleaved Parp1 (116-kD fragment). β-Actin was used as loading control. Data are represented as mean ± SEM. * P
    In Vitro Reverse Transcription Polymerase Chain Reaction Rt Pcr Assay, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche reverse transcription polymerase chain reaction assays rt pcr
    Constitutive activation of β-catenin induces p53-activation and apoptosis in small intestine explants from ApcZfp148 mice A. - B. <t>Taqman</t> <t>RT-PCR</t> assessment of mRNA levels of three β-catenin target genes A. and four p53-target genes B. in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM of the GSK3β-inhibitor GSK3IX or DMSO. The explants in A. were treated for 6 hours and those in B. for 20 hours ( n = 6). C. Western blots of phosphorylated and total p53, the p53 targets p21 and Bax, and the apoptosis marker cleaved Parp1 (89-kD fragment) in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM GSK3IX or DMSO for 20 hours. Asterisk indicates uncleaved Parp1 (116-kD fragment). β-Actin was used as loading control. Data are represented as mean ± SEM. * P
    Reverse Transcription Polymerase Chain Reaction Assays Rt Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Toyobo reverse transcription polymerase chain reaction rt pcr assay
    Constitutive activation of β-catenin induces p53-activation and apoptosis in small intestine explants from ApcZfp148 mice A. - B. <t>Taqman</t> <t>RT-PCR</t> assessment of mRNA levels of three β-catenin target genes A. and four p53-target genes B. in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM of the GSK3β-inhibitor GSK3IX or DMSO. The explants in A. were treated for 6 hours and those in B. for 20 hours ( n = 6). C. Western blots of phosphorylated and total p53, the p53 targets p21 and Bax, and the apoptosis marker cleaved Parp1 (89-kD fragment) in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM GSK3IX or DMSO for 20 hours. Asterisk indicates uncleaved Parp1 (116-kD fragment). β-Actin was used as loading control. Data are represented as mean ± SEM. * P
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Assay, supplied by Toyobo, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa reverse transcription polymerase chain reaction rt pcr assays
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
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    GenMark Diagnostics multiplex reverse transcription polymerase chain reaction rt pcr assay
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Multiplex Reverse Transcription Polymerase Chain Reaction Rt Pcr Assay, supplied by GenMark Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa reverse transcription polymerase chain reaction rt pcr assay kit
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Assay Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche qualitative reverse transcription polymerase chain reaction rt pcr assay
    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of <t>ID1,</t> ID2, or ID3. ( B ) Real-time <t>PCR</t> analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P
    Qualitative Reverse Transcription Polymerase Chain Reaction Rt Pcr Assay, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega one step reverse transcription polymerase chain reaction rt pcr assay
    <t>MXI1-0</t> is overexpressed in glioblastoma tumors. Semiquantitative multiplex <t>RT-PCR</t> and standard RT-PCR with GAPDH-specific primers was performed with RNA from 7 normal brain specimens and 10 glioblastoma multiforme tumors. The numbers above the ethidium bromide-stained gel indicate the ratio of MXI1-0 to MXI1 band intensity (normalized to GAPDH), with mean MXI1-0/MXI1 (Ex 0/Ex 1) ratio as indicated.
    One Step Reverse Transcription Polymerase Chain Reaction Rt Pcr Assay, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reverse transcription polymerase chain reaction rt pcr assay trizol reagent
    <t>MXI1-0</t> is overexpressed in glioblastoma tumors. Semiquantitative multiplex <t>RT-PCR</t> and standard RT-PCR with GAPDH-specific primers was performed with RNA from 7 normal brain specimens and 10 glioblastoma multiforme tumors. The numbers above the ethidium bromide-stained gel indicate the ratio of MXI1-0 to MXI1 band intensity (normalized to GAPDH), with mean MXI1-0/MXI1 (Ex 0/Ex 1) ratio as indicated.
    Reverse Transcription Polymerase Chain Reaction Rt Pcr Assay Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative real time reverse transcription polymerase chain reaction rt pcr assay
    AEG-1 and MDR-1 expression in <t>HCC</t> and adjacent normal tissues AEG-1 and MDR-1 expression in HCC and adjacent normal tissues were investigated by immunohistochemical staining (A) and <t>RT-PCR</t> assays (B). AEG-1: Astrocyte elevated gene-1; MDR-1: multiple drug resistance gene-1; Cancer: Hepatocellular carcinoma; Normal: matched adjacent normal liver tissues. * P
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    Seegene real time reverse transcription polymerase chain reaction rt pcr assay
    AEG-1 and MDR-1 expression in <t>HCC</t> and adjacent normal tissues AEG-1 and MDR-1 expression in HCC and adjacent normal tissues were investigated by immunohistochemical staining (A) and <t>RT-PCR</t> assays (B). AEG-1: Astrocyte elevated gene-1; MDR-1: multiple drug resistance gene-1; Cancer: Hepatocellular carcinoma; Normal: matched adjacent normal liver tissues. * P
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    AEG-1 and MDR-1 expression in <t>HCC</t> and adjacent normal tissues AEG-1 and MDR-1 expression in HCC and adjacent normal tissues were investigated by immunohistochemical staining (A) and <t>RT-PCR</t> assays (B). AEG-1: Astrocyte elevated gene-1; MDR-1: multiple drug resistance gene-1; Cancer: Hepatocellular carcinoma; Normal: matched adjacent normal liver tissues. * P
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    Roche prevention cdc reverse transcription polymerase chain reaction rt pcr sars cov 2 assay
    AEG-1 and MDR-1 expression in <t>HCC</t> and adjacent normal tissues AEG-1 and MDR-1 expression in HCC and adjacent normal tissues were investigated by immunohistochemical staining (A) and <t>RT-PCR</t> assays (B). AEG-1: Astrocyte elevated gene-1; MDR-1: multiple drug resistance gene-1; Cancer: Hepatocellular carcinoma; Normal: matched adjacent normal liver tissues. * P
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    AEG-1 and MDR-1 expression in <t>HCC</t> and adjacent normal tissues AEG-1 and MDR-1 expression in HCC and adjacent normal tissues were investigated by immunohistochemical staining (A) and <t>RT-PCR</t> assays (B). AEG-1: Astrocyte elevated gene-1; MDR-1: multiple drug resistance gene-1; Cancer: Hepatocellular carcinoma; Normal: matched adjacent normal liver tissues. * P
    Reverse Transcription Polymerase Chain Reaction Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reverse transcription polymerase chain reaction rt pcr trizol
    AEG-1 and MDR-1 expression in <t>HCC</t> and adjacent normal tissues AEG-1 and MDR-1 expression in HCC and adjacent normal tissues were investigated by immunohistochemical staining (A) and <t>RT-PCR</t> assays (B). AEG-1: Astrocyte elevated gene-1; MDR-1: multiple drug resistance gene-1; Cancer: Hepatocellular carcinoma; Normal: matched adjacent normal liver tissues. * P
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    Image Search Results


    Constitutive activation of β-catenin induces p53-activation and apoptosis in small intestine explants from ApcZfp148 mice A. - B. Taqman RT-PCR assessment of mRNA levels of three β-catenin target genes A. and four p53-target genes B. in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM of the GSK3β-inhibitor GSK3IX or DMSO. The explants in A. were treated for 6 hours and those in B. for 20 hours ( n = 6). C. Western blots of phosphorylated and total p53, the p53 targets p21 and Bax, and the apoptosis marker cleaved Parp1 (89-kD fragment) in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM GSK3IX or DMSO for 20 hours. Asterisk indicates uncleaved Parp1 (116-kD fragment). β-Actin was used as loading control. Data are represented as mean ± SEM. * P

    Journal: Oncotarget

    Article Title: Targeting Zfp148 activates p53 and reduces tumor initiation in the gut

    doi: 10.18632/oncotarget.10899

    Figure Lengend Snippet: Constitutive activation of β-catenin induces p53-activation and apoptosis in small intestine explants from ApcZfp148 mice A. - B. Taqman RT-PCR assessment of mRNA levels of three β-catenin target genes A. and four p53-target genes B. in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM of the GSK3β-inhibitor GSK3IX or DMSO. The explants in A. were treated for 6 hours and those in B. for 20 hours ( n = 6). C. Western blots of phosphorylated and total p53, the p53 targets p21 and Bax, and the apoptosis marker cleaved Parp1 (89-kD fragment) in small intestine explants that were dissected from Apc and ApcZfp148 mice and treated with 2μM GSK3IX or DMSO for 20 hours. Asterisk indicates uncleaved Parp1 (116-kD fragment). β-Actin was used as loading control. Data are represented as mean ± SEM. * P

    Article Snippet: Real-time reverse transcription polymerase chain reaction (RT-PCR) TaqMan assays were performed as described [ ] using TaqMan universal polymerase chain reaction mastermix (Life Technologies) and the predesigned TaqMan assays (Life Technologies) listed in .

    Techniques: Activation Assay, Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Marker

    ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of ID1, ID2, or ID3. ( B ) Real-time PCR analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P

    Journal: OncoTargets and therapy

    Article Title: ID2 predicts poor prognosis in breast cancer, especially in triple-negative breast cancer, and inhibits E-cadherin expression

    doi: 10.2147/OTT.S64759

    Figure Lengend Snippet: ID proteins inhibit E-cadherin expression in breast cells. Notes: ( A ) Western blot analysis of MCF10A cells with stable expression of ID1, ID2, or ID3. ( B ) Real-time PCR analysis of E-cadherin mRNA abundance in cell lines in ( A ). ( C ) Western blot analysis of E-cadherin protein abundance in cell lines in ( A ). ( D ) Assessment of E-cadherin promoter activity, using luciferase reporter assays, in HEK293T cells. The 1.38 kb DNA sequence containing E-cadherin promoter region was cloned upstream of the luciferase gene in a reporter construct (top image). The relative luciferase activities of luciferase reporters with E-cadherin promoter were determined in HEK293T cells, which were cotransfected with the ID1, ID2, ID3, and control vectors. For ( B ) and ( D , bottom image), data represent mean values, with error bars indicating SEM. ** P

    Article Snippet: Plasmid constructs The human full-length coding sequences of ID1, ID2, and ID3 were cloned using a standard reverse-transcription polymerase chain reaction (RT-PCR) protocol (Takara Bio Company, Dalian, Japan).

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Activity Assay, Luciferase, Sequencing, Clone Assay, Construct

    Changes in BCL-2 family expression caused by rapamycin treatment and FAK down-regulation. REH-empty vector or REH-FAK shRNA cells were cultured with or without rapamycin (100 nM). After 10 h, RNA was extracted from these cells and mRNA expression was detected using quantitative real-time PCR. The expression of pro-apoptosis genes, such as BIK, PUMA, BMF, BAX, and MCL-1S, was higher in the combination treatment group than in the rapamycin-only group. The expression of anti-apoptosis genes, such as BCL-2, was decreased in the combination treatment group compared with the rapamycin-only group. The results represent the mean ± S.D. of three experiments performed in triplicate. * p

    Journal: Journal of Hematology & Oncology

    Article Title: Synergism between the mTOR inhibitor rapamycin and FAK down-regulation in the treatment of acute lymphoblastic leukemia

    doi: 10.1186/s13045-016-0241-x

    Figure Lengend Snippet: Changes in BCL-2 family expression caused by rapamycin treatment and FAK down-regulation. REH-empty vector or REH-FAK shRNA cells were cultured with or without rapamycin (100 nM). After 10 h, RNA was extracted from these cells and mRNA expression was detected using quantitative real-time PCR. The expression of pro-apoptosis genes, such as BIK, PUMA, BMF, BAX, and MCL-1S, was higher in the combination treatment group than in the rapamycin-only group. The expression of anti-apoptosis genes, such as BCL-2, was decreased in the combination treatment group compared with the rapamycin-only group. The results represent the mean ± S.D. of three experiments performed in triplicate. * p

    Article Snippet: Total RNA was extracted using the TRIzol reagent (Invitrogen, USA) according to the manufacturer’s protocols. cDNA was prepared from 1 μg of total RNA using a reverse transcription-polymerase chain reaction (RT-PCR) kit (Takara, Japan) with oligodT according to the manufacturer’s instructions. cDNA samples were then analyzed via quantitative real-time PCR using SYBR Green (Takara, Japan) in an ABI Step One Real-Time PCR machine (Applied Biosystems, Foster City, CA), with 40 cycles of 95 °C for 15 s and 60 °C for 30 s. The efficiency of cDNA synthesis was estimated using hGAPDH as a house-keeping gene.

    Techniques: Expressing, Plasmid Preparation, shRNA, Cell Culture, Real-time Polymerase Chain Reaction

    MXI1-0 is overexpressed in glioblastoma tumors. Semiquantitative multiplex RT-PCR and standard RT-PCR with GAPDH-specific primers was performed with RNA from 7 normal brain specimens and 10 glioblastoma multiforme tumors. The numbers above the ethidium bromide-stained gel indicate the ratio of MXI1-0 to MXI1 band intensity (normalized to GAPDH), with mean MXI1-0/MXI1 (Ex 0/Ex 1) ratio as indicated.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: MXI1-0, an Alternatively Transcribed Mxi1 Isoform, Is Overexpressed in Glioblastomas

    doi:

    Figure Lengend Snippet: MXI1-0 is overexpressed in glioblastoma tumors. Semiquantitative multiplex RT-PCR and standard RT-PCR with GAPDH-specific primers was performed with RNA from 7 normal brain specimens and 10 glioblastoma multiforme tumors. The numbers above the ethidium bromide-stained gel indicate the ratio of MXI1-0 to MXI1 band intensity (normalized to GAPDH), with mean MXI1-0/MXI1 (Ex 0/Ex 1) ratio as indicated.

    Article Snippet: An MXI1/MXI1-0 -specific multiplex, one-step reverse transcription-polymerase chain reaction (RT-PCR) assay (Access RT-PCR, Promega) was set up using separate forward primers for exon 0 (5′-GACATTTTCAACACCAGCGAGAA CTCGATG-3′) and exon 1 (5′-CAACGTGCAGCGTCTGCTGGAGGC-3′) and a common reverse primer from exon 3 (5′-CGATTCTTTTCCAGCTCATTGTG-3′) ( ).

    Techniques: Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Staining

    ] and MXI1-0 mRNA signal is comparable in size. (B) Schematic of multiplex RT-PCR. Solid black bar indicates exon 0, white bar is exon 1, diagonal black striped bar is exon 2, gray bar is exon 3, and dotted bar is exon 4. Forward primers specific for exon 0 (Ex0-F) and exon 1 (Ex1-F) and a common reverse primer from exon 3 (Ex3-R) are used to amplify 281- and 216-bp products, respectively. (C) MXI1-0 and MXI1 are both expressed in both human and mouse cDNA. PCR was performed with MXI1-0- and MXI1-specific forward primers and a common reverse primer, using human heart (H) or mouse pGAB (M) cDNA libraries (gift of G. Nunez). Both MXI1-0 and MXI1 bands are amplified from each cDNA source.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: MXI1-0, an Alternatively Transcribed Mxi1 Isoform, Is Overexpressed in Glioblastomas

    doi:

    Figure Lengend Snippet: ] and MXI1-0 mRNA signal is comparable in size. (B) Schematic of multiplex RT-PCR. Solid black bar indicates exon 0, white bar is exon 1, diagonal black striped bar is exon 2, gray bar is exon 3, and dotted bar is exon 4. Forward primers specific for exon 0 (Ex0-F) and exon 1 (Ex1-F) and a common reverse primer from exon 3 (Ex3-R) are used to amplify 281- and 216-bp products, respectively. (C) MXI1-0 and MXI1 are both expressed in both human and mouse cDNA. PCR was performed with MXI1-0- and MXI1-specific forward primers and a common reverse primer, using human heart (H) or mouse pGAB (M) cDNA libraries (gift of G. Nunez). Both MXI1-0 and MXI1 bands are amplified from each cDNA source.

    Article Snippet: An MXI1/MXI1-0 -specific multiplex, one-step reverse transcription-polymerase chain reaction (RT-PCR) assay (Access RT-PCR, Promega) was set up using separate forward primers for exon 0 (5′-GACATTTTCAACACCAGCGAGAA CTCGATG-3′) and exon 1 (5′-CAACGTGCAGCGTCTGCTGGAGGC-3′) and a common reverse primer from exon 3 (5′-CGATTCTTTTCCAGCTCATTGTG-3′) ( ).

    Techniques: Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification

    AEG-1 and MDR-1 expression in HCC and adjacent normal tissues AEG-1 and MDR-1 expression in HCC and adjacent normal tissues were investigated by immunohistochemical staining (A) and RT-PCR assays (B). AEG-1: Astrocyte elevated gene-1; MDR-1: multiple drug resistance gene-1; Cancer: Hepatocellular carcinoma; Normal: matched adjacent normal liver tissues. * P

    Journal: EXCLI Journal

    Article Title: AEG-1 is associated with hypoxia-induced hepatocellular carcinoma chemoresistance via regulating PI3K/AKT/HIF-1alpha/MDR-1 pathway

    doi: 10.17179/excli2016-694

    Figure Lengend Snippet: AEG-1 and MDR-1 expression in HCC and adjacent normal tissues AEG-1 and MDR-1 expression in HCC and adjacent normal tissues were investigated by immunohistochemical staining (A) and RT-PCR assays (B). AEG-1: Astrocyte elevated gene-1; MDR-1: multiple drug resistance gene-1; Cancer: Hepatocellular carcinoma; Normal: matched adjacent normal liver tissues. * P

    Article Snippet: Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) assay The HCC and adjacent normal tissue samples were retrieved from liquid nitrogen and grinded to tiny particles, before incubation with TRIzol (Takara, Otsu, Shiga, Japan) to isolated mRNA.

    Techniques: Expressing, Immunohistochemistry, Staining, Reverse Transcription Polymerase Chain Reaction