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    LC Laboratories 17 aag
    Mitochondriotoxic mechanism of action of Gamitrinibs in prostate cancer cells A, PC3 cells were double-labeled with TMRM (red fluorescence, mitochondrial membrane potential, MMP) and MitoTracker (green fluorescence, mitochondria), treated with G-TPP and analyzed by time-lapse videomicroscopy. Representative sequential images after treatment are shown. 1 frame = 5 min. Arrowheads , PC3 cells that have lost MMP. B, TMRM-labeled mitochondria isolated from PC3 cells were incubated with <t>17-AAG,</t> G-TPP or G-G4 in the presence or absence of CSA, and analyzed for changes in mitochondrial membrane potential in a fluorimeter. C, Mitochondria from PC3 or normal prostatic epithelial BPH-1 cells were treated with 17-AAG or G-TPP, and analyzed for cytochrome c content in pellets (P) or supernatants (S). D, PC3 cells were transfected with control (Ctrl) non-targeting or Bax and Bak-directed siRNA, and analyzed by Western blotting. None, untreated. E, siRNA transfected PC3 cells were treated as indicated and analyzed by MTT. Mean±SEM of replicates.
    17 Aag, supplied by LC Laboratories, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore 17 allylamino 17 demethoxygeldanamycin
    Mitochondriotoxic mechanism of action of Gamitrinibs in prostate cancer cells A, PC3 cells were double-labeled with TMRM (red fluorescence, mitochondrial membrane potential, MMP) and MitoTracker (green fluorescence, mitochondria), treated with G-TPP and analyzed by time-lapse videomicroscopy. Representative sequential images after treatment are shown. 1 frame = 5 min. Arrowheads , PC3 cells that have lost MMP. B, TMRM-labeled mitochondria isolated from PC3 cells were incubated with <t>17-AAG,</t> G-TPP or G-G4 in the presence or absence of CSA, and analyzed for changes in mitochondrial membrane potential in a fluorimeter. C, Mitochondria from PC3 or normal prostatic epithelial BPH-1 cells were treated with 17-AAG or G-TPP, and analyzed for cytochrome c content in pellets (P) or supernatants (S). D, PC3 cells were transfected with control (Ctrl) non-targeting or Bax and Bak-directed siRNA, and analyzed by Western blotting. None, untreated. E, siRNA transfected PC3 cells were treated as indicated and analyzed by MTT. Mean±SEM of replicates.
    17 Allylamino 17 Demethoxygeldanamycin, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    InvivoGen 17 allylamino 17 demethoxygeldanamycin
    Mitochondriotoxic mechanism of action of Gamitrinibs in prostate cancer cells A, PC3 cells were double-labeled with TMRM (red fluorescence, mitochondrial membrane potential, MMP) and MitoTracker (green fluorescence, mitochondria), treated with G-TPP and analyzed by time-lapse videomicroscopy. Representative sequential images after treatment are shown. 1 frame = 5 min. Arrowheads , PC3 cells that have lost MMP. B, TMRM-labeled mitochondria isolated from PC3 cells were incubated with <t>17-AAG,</t> G-TPP or G-G4 in the presence or absence of CSA, and analyzed for changes in mitochondrial membrane potential in a fluorimeter. C, Mitochondria from PC3 or normal prostatic epithelial BPH-1 cells were treated with 17-AAG or G-TPP, and analyzed for cytochrome c content in pellets (P) or supernatants (S). D, PC3 cells were transfected with control (Ctrl) non-targeting or Bax and Bak-directed siRNA, and analyzed by Western blotting. None, untreated. E, siRNA transfected PC3 cells were treated as indicated and analyzed by MTT. Mean±SEM of replicates.
    17 Allylamino 17 Demethoxygeldanamycin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/17 allylamino 17 demethoxygeldanamycin/product/InvivoGen
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    94
    Selleck Chemicals 17 aag
    Mitochondriotoxic mechanism of action of Gamitrinibs in prostate cancer cells A, PC3 cells were double-labeled with TMRM (red fluorescence, mitochondrial membrane potential, MMP) and MitoTracker (green fluorescence, mitochondria), treated with G-TPP and analyzed by time-lapse videomicroscopy. Representative sequential images after treatment are shown. 1 frame = 5 min. Arrowheads , PC3 cells that have lost MMP. B, TMRM-labeled mitochondria isolated from PC3 cells were incubated with <t>17-AAG,</t> G-TPP or G-G4 in the presence or absence of CSA, and analyzed for changes in mitochondrial membrane potential in a fluorimeter. C, Mitochondria from PC3 or normal prostatic epithelial BPH-1 cells were treated with 17-AAG or G-TPP, and analyzed for cytochrome c content in pellets (P) or supernatants (S). D, PC3 cells were transfected with control (Ctrl) non-targeting or Bax and Bak-directed siRNA, and analyzed by Western blotting. None, untreated. E, siRNA transfected PC3 cells were treated as indicated and analyzed by MTT. Mean±SEM of replicates.
    17 Aag, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Mitochondriotoxic mechanism of action of Gamitrinibs in prostate cancer cells A, PC3 cells were double-labeled with TMRM (red fluorescence, mitochondrial membrane potential, MMP) and MitoTracker (green fluorescence, mitochondria), treated with G-TPP and analyzed by time-lapse videomicroscopy. Representative sequential images after treatment are shown. 1 frame = 5 min. Arrowheads , PC3 cells that have lost MMP. B, TMRM-labeled mitochondria isolated from PC3 cells were incubated with 17-AAG, G-TPP or G-G4 in the presence or absence of CSA, and analyzed for changes in mitochondrial membrane potential in a fluorimeter. C, Mitochondria from PC3 or normal prostatic epithelial BPH-1 cells were treated with 17-AAG or G-TPP, and analyzed for cytochrome c content in pellets (P) or supernatants (S). D, PC3 cells were transfected with control (Ctrl) non-targeting or Bax and Bak-directed siRNA, and analyzed by Western blotting. None, untreated. E, siRNA transfected PC3 cells were treated as indicated and analyzed by MTT. Mean±SEM of replicates.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: PRECLINICAL CHARACTERIZATION OF MITOCHONDRIA-TARGETED SMALL MOLECULE Hsp90 INHIBITORS, GAMITRINIBS, IN ADVANCED PROSTATE CANCER

    doi: 10.1158/1078-0432.CCR-10-1818

    Figure Lengend Snippet: Mitochondriotoxic mechanism of action of Gamitrinibs in prostate cancer cells A, PC3 cells were double-labeled with TMRM (red fluorescence, mitochondrial membrane potential, MMP) and MitoTracker (green fluorescence, mitochondria), treated with G-TPP and analyzed by time-lapse videomicroscopy. Representative sequential images after treatment are shown. 1 frame = 5 min. Arrowheads , PC3 cells that have lost MMP. B, TMRM-labeled mitochondria isolated from PC3 cells were incubated with 17-AAG, G-TPP or G-G4 in the presence or absence of CSA, and analyzed for changes in mitochondrial membrane potential in a fluorimeter. C, Mitochondria from PC3 or normal prostatic epithelial BPH-1 cells were treated with 17-AAG or G-TPP, and analyzed for cytochrome c content in pellets (P) or supernatants (S). D, PC3 cells were transfected with control (Ctrl) non-targeting or Bax and Bak-directed siRNA, and analyzed by Western blotting. None, untreated. E, siRNA transfected PC3 cells were treated as indicated and analyzed by MTT. Mean±SEM of replicates.

    Article Snippet: Here, protracted exposure of PC3 cells to 17-AAG, i.e. PC3-GA cells, selectively upregulated the expression of P-gp , whereas other ABC transporters or various cytoprotective chaperones implicated in drug resistance mechanisms in prostate cancer ( ) were not affected.

    Techniques: Labeling, Fluorescence, Isolation, Incubation, Transfection, Western Blot, MTT Assay

    Activity of Gamitrinib in an orthotopic model of bone metastatic prostate cancer A, CB17 SCID/beige mice injected in the tibiae with PC3 cells were treated with G-TPP or vehicle (DMSO), and limbs of representative animals in each group (#) were analyzed by µCT. B, Percent change in bone volume (BV)/total volume (TV) ratio in individual animals ( top ) or groups ( middle ) treated with DMSO or G-TPP, as determined by µCT reconstruction of bone lesions. Bottom , percent BV of tibial tumor limb (TT) or contralateral tibia (CT). Mean±SEM of individual determinations. C, PC3 cells were treated with G-TPP or 17-AAG for the indicated time intervals, and analyzed by Western blotting.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: PRECLINICAL CHARACTERIZATION OF MITOCHONDRIA-TARGETED SMALL MOLECULE Hsp90 INHIBITORS, GAMITRINIBS, IN ADVANCED PROSTATE CANCER

    doi: 10.1158/1078-0432.CCR-10-1818

    Figure Lengend Snippet: Activity of Gamitrinib in an orthotopic model of bone metastatic prostate cancer A, CB17 SCID/beige mice injected in the tibiae with PC3 cells were treated with G-TPP or vehicle (DMSO), and limbs of representative animals in each group (#) were analyzed by µCT. B, Percent change in bone volume (BV)/total volume (TV) ratio in individual animals ( top ) or groups ( middle ) treated with DMSO or G-TPP, as determined by µCT reconstruction of bone lesions. Bottom , percent BV of tibial tumor limb (TT) or contralateral tibia (CT). Mean±SEM of individual determinations. C, PC3 cells were treated with G-TPP or 17-AAG for the indicated time intervals, and analyzed by Western blotting.

    Article Snippet: Here, protracted exposure of PC3 cells to 17-AAG, i.e. PC3-GA cells, selectively upregulated the expression of P-gp , whereas other ABC transporters or various cytoprotective chaperones implicated in drug resistance mechanisms in prostate cancer ( ) were not affected.

    Techniques: Activity Assay, Mouse Assay, Injection, Western Blot

    Anticancer activity of Gamitrinibs A, Gamitrinib-G4 (G-G4) or Gamitrinib-TPP (G-TPP) were analyzed for inhibition of cell proliferation in a NCI 60-cell line screening by MTT. Each line corresponds to a different tumor cell type. B, Prostate cancer PC3 ( top ) or C4-2B ( bottom ) cells were incubated with the indicated increasing concentrations of Gamitrinibs (G1–G4, G-TPP) or 17-AAG and analyzed after 6 h ( left ) or 24 h ( right ), by MTT. Mean±SEM of replicates. C, PC3 cells were incubated with G-TPP or 17-AAG, and analyzed for DEVDase (caspase) activity and propidium iodide (PI) staining by multiparametric flow cytometry. The percentage of cells in each quadrant is indicated. None, untreated. D, PC3 cells were treated with G-TPP or 17-AAG for the indicated time intervals, and analyzed by Western blotting.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: PRECLINICAL CHARACTERIZATION OF MITOCHONDRIA-TARGETED SMALL MOLECULE Hsp90 INHIBITORS, GAMITRINIBS, IN ADVANCED PROSTATE CANCER

    doi: 10.1158/1078-0432.CCR-10-1818

    Figure Lengend Snippet: Anticancer activity of Gamitrinibs A, Gamitrinib-G4 (G-G4) or Gamitrinib-TPP (G-TPP) were analyzed for inhibition of cell proliferation in a NCI 60-cell line screening by MTT. Each line corresponds to a different tumor cell type. B, Prostate cancer PC3 ( top ) or C4-2B ( bottom ) cells were incubated with the indicated increasing concentrations of Gamitrinibs (G1–G4, G-TPP) or 17-AAG and analyzed after 6 h ( left ) or 24 h ( right ), by MTT. Mean±SEM of replicates. C, PC3 cells were incubated with G-TPP or 17-AAG, and analyzed for DEVDase (caspase) activity and propidium iodide (PI) staining by multiparametric flow cytometry. The percentage of cells in each quadrant is indicated. None, untreated. D, PC3 cells were treated with G-TPP or 17-AAG for the indicated time intervals, and analyzed by Western blotting.

    Article Snippet: Here, protracted exposure of PC3 cells to 17-AAG, i.e. PC3-GA cells, selectively upregulated the expression of P-gp , whereas other ABC transporters or various cytoprotective chaperones implicated in drug resistance mechanisms in prostate cancer ( ) were not affected.

    Techniques: Activity Assay, Inhibition, MTT Assay, Incubation, Staining, Flow Cytometry, Cytometry, Western Blot

    Activity of Gamitrinib against multidrug-resistant prostate cancer A, PC3 ( black ) or PC3-GA ( purple ) cells were treated with the indicated concentrations of 17-AAG ( left ) or taxol ( right ), and analyzed by MTT in the presence ( solid symbols ) or absence ( open symbols ) of verapamil (20 µM). B, PC3 or PC3-GA cells were analyzed by PCR, and amplified bands were visualized by ethidium bromide staining ( left ), and quantified ( right ). RT, reverse transcriptase. C, PC3 or PC3-GA cells were analyzed by Western blotting before or after treatment with 17-AAG. D, The experimental conditions are as in A, except that PC3 or PC3-GA cells were treated with G-G4 ( left ) or G-TPP ( right ) and analyzed by MTT. For panels, A, B and D, mean±SEM of replicates.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: PRECLINICAL CHARACTERIZATION OF MITOCHONDRIA-TARGETED SMALL MOLECULE Hsp90 INHIBITORS, GAMITRINIBS, IN ADVANCED PROSTATE CANCER

    doi: 10.1158/1078-0432.CCR-10-1818

    Figure Lengend Snippet: Activity of Gamitrinib against multidrug-resistant prostate cancer A, PC3 ( black ) or PC3-GA ( purple ) cells were treated with the indicated concentrations of 17-AAG ( left ) or taxol ( right ), and analyzed by MTT in the presence ( solid symbols ) or absence ( open symbols ) of verapamil (20 µM). B, PC3 or PC3-GA cells were analyzed by PCR, and amplified bands were visualized by ethidium bromide staining ( left ), and quantified ( right ). RT, reverse transcriptase. C, PC3 or PC3-GA cells were analyzed by Western blotting before or after treatment with 17-AAG. D, The experimental conditions are as in A, except that PC3 or PC3-GA cells were treated with G-G4 ( left ) or G-TPP ( right ) and analyzed by MTT. For panels, A, B and D, mean±SEM of replicates.

    Article Snippet: Here, protracted exposure of PC3 cells to 17-AAG, i.e. PC3-GA cells, selectively upregulated the expression of P-gp , whereas other ABC transporters or various cytoprotective chaperones implicated in drug resistance mechanisms in prostate cancer ( ) were not affected.

    Techniques: Activity Assay, MTT Assay, Polymerase Chain Reaction, Amplification, Staining, Western Blot