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  • 84
    GE Healthcare reversed phase ge healthcare resource rpc
    Reversed Phase Ge Healthcare Resource Rpc, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 84/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reversed phase ge healthcare resource rpc/product/GE Healthcare
    Average 84 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
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    94
    GE Healthcare resource rpc
    Biophysical characterization of PAS#1(600)-hGH. ( A ) Mass spectrometric analysis of PAS#1(600)-hGH by ESI-MS, confirming the calculated molecular mass of 73121.65 Da as well as precisely monodisperse composition, without any indication of prematurely terminated gene products. ( B ) Hydrophobicity analysis of PASylated hGH by reverse-phase (RP) HPLC. HIC of the original recombinant hGH and its PASylated version was performed on a Resource <t>RPC</t> column using an elution gradient from 2% v/v acetonitrile, 0.065% v/v <t>TFA</t> to 80% v/v acetonitrile, 0.05% v/v TFA. Both profiles show a single homogeneous peak with slightly earlier elution of PAS#1(600)-hGH (51.8% acetonitrile) in comparison with the corresponding unfused protein (52.3% acetonitrile). ( C ) Thermal denaturation of hGH and PAS#1(600)-hGH as determined by far UV CD measurement. Thermal unfolding of a 8.2 µM protein solution in 50 mM K 2 SO 4 , 20 mM K-P i pH 7.5 was followed at a wavelength of 208 nm where maximal spectral change upon unfolding was observed and the normalized unfolded fraction, f(u), was plotted as a function of temperature. The melting temperatures ( T m ) of hGH and PAS#1(600)-hGH were 85.4°C and 88.6°C, respectively.
    Resource Rpc, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resource rpc/product/GE Healthcare
    Average 94 stars, based on 126 article reviews
    Price from $9.99 to $1999.99
    resource rpc - by Bioz Stars, 2020-02
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    97
    GE Healthcare resource rpc column
    Reverse-phase HPLC profile of lacticin Q produced by L. lactis QU 5. Lacticin Q was isolated using a 3-ml <t>RESOURCE</t> <t>RPC</t> column (Amersham Biosciences) at a flow rate of 1.0 ml/min. Antibacterial activity was detected in the fractions indicated by a double-headed
    Resource Rpc Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/resource rpc column/product/GE Healthcare
    Average 97 stars, based on 245 article reviews
    Price from $9.99 to $1999.99
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    79
    GE Healthcare resource 15 rpc column
    Reverse-phase HPLC profile of lacticin Q produced by L. lactis QU 5. Lacticin Q was isolated using a 3-ml <t>RESOURCE</t> <t>RPC</t> column (Amersham Biosciences) at a flow rate of 1.0 ml/min. Antibacterial activity was detected in the fractions indicated by a double-headed
    Resource 15 Rpc Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    GE Healthcare resource rpc analytical column
    Reverse-phase HPLC profile of lacticin Q produced by L. lactis QU 5. Lacticin Q was isolated using a 3-ml <t>RESOURCE</t> <t>RPC</t> column (Amersham Biosciences) at a flow rate of 1.0 ml/min. Antibacterial activity was detected in the fractions indicated by a double-headed
    Resource Rpc Analytical Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    GE Healthcare reversed phase resource rpc column
    Reverse-phase HPLC profile of lacticin Q produced by L. lactis QU 5. Lacticin Q was isolated using a 3-ml <t>RESOURCE</t> <t>RPC</t> column (Amersham Biosciences) at a flow rate of 1.0 ml/min. Antibacterial activity was detected in the fractions indicated by a double-headed
    Reversed Phase Resource Rpc Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reversed phase resource rpc column/product/GE Healthcare
    Average 90 stars, based on 21 article reviews
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    82
    GE Healthcare resource rpc fplc column
    Reverse-phase HPLC profile of lacticin Q produced by L. lactis QU 5. Lacticin Q was isolated using a 3-ml <t>RESOURCE</t> <t>RPC</t> column (Amersham Biosciences) at a flow rate of 1.0 ml/min. Antibacterial activity was detected in the fractions indicated by a double-headed
    Resource Rpc Fplc Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 82/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 82 stars, based on 31 article reviews
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    79
    GE Healthcare resource rpc reverse phase column
    Reverse-phase HPLC profile of lacticin Q produced by L. lactis QU 5. Lacticin Q was isolated using a 3-ml <t>RESOURCE</t> <t>RPC</t> column (Amersham Biosciences) at a flow rate of 1.0 ml/min. Antibacterial activity was detected in the fractions indicated by a double-headed
    Resource Rpc Reverse Phase Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    GE Healthcare resource reversed phase chromatography rpc column
    Purification of SAMP H1. (A) <t>HiPrep</t> SP cation-exchange chromatography of SepPac C 18 -concentrated skin mucus extract; (B) Mono S cation-exchange chromatography with urea of <t>RPC-concentrated</t> active fractions obtained upon HiPrep SP chromatography; (C) final
    Resource Reversed Phase Chromatography Rpc Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare resource rpc reversed phase fplc column
    Purification of SAMP H1. (A) <t>HiPrep</t> SP cation-exchange chromatography of SepPac C 18 -concentrated skin mucus extract; (B) Mono S cation-exchange chromatography with urea of <t>RPC-concentrated</t> active fractions obtained upon HiPrep SP chromatography; (C) final
    Resource Rpc Reversed Phase Fplc Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 7 article reviews
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    87
    GE Healthcare reverse phase chromatography column
    Purification of SAMP H1. (A) <t>HiPrep</t> SP cation-exchange chromatography of SepPac C 18 -concentrated skin mucus extract; (B) Mono S cation-exchange chromatography with urea of <t>RPC-concentrated</t> active fractions obtained upon HiPrep SP chromatography; (C) final
    Reverse Phase Chromatography Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 87/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    GE Healthcare phase chromatography
    Purification of SAMP H1. (A) <t>HiPrep</t> SP cation-exchange chromatography of SepPac C 18 -concentrated skin mucus extract; (B) Mono S cation-exchange chromatography with urea of <t>RPC-concentrated</t> active fractions obtained upon HiPrep SP chromatography; (C) final
    Phase Chromatography, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    GE Healthcare reversed phase column
    Purification of SAMP H1. (A) <t>HiPrep</t> SP cation-exchange chromatography of SepPac C 18 -concentrated skin mucus extract; (B) Mono S cation-exchange chromatography with urea of <t>RPC-concentrated</t> active fractions obtained upon HiPrep SP chromatography; (C) final
    Reversed Phase Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reversed phase column/product/GE Healthcare
    Average 89 stars, based on 11 article reviews
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    Image Search Results


    Biophysical characterization of PAS#1(600)-hGH. ( A ) Mass spectrometric analysis of PAS#1(600)-hGH by ESI-MS, confirming the calculated molecular mass of 73121.65 Da as well as precisely monodisperse composition, without any indication of prematurely terminated gene products. ( B ) Hydrophobicity analysis of PASylated hGH by reverse-phase (RP) HPLC. HIC of the original recombinant hGH and its PASylated version was performed on a Resource RPC column using an elution gradient from 2% v/v acetonitrile, 0.065% v/v TFA to 80% v/v acetonitrile, 0.05% v/v TFA. Both profiles show a single homogeneous peak with slightly earlier elution of PAS#1(600)-hGH (51.8% acetonitrile) in comparison with the corresponding unfused protein (52.3% acetonitrile). ( C ) Thermal denaturation of hGH and PAS#1(600)-hGH as determined by far UV CD measurement. Thermal unfolding of a 8.2 µM protein solution in 50 mM K 2 SO 4 , 20 mM K-P i pH 7.5 was followed at a wavelength of 208 nm where maximal spectral change upon unfolding was observed and the normalized unfolded fraction, f(u), was plotted as a function of temperature. The melting temperatures ( T m ) of hGH and PAS#1(600)-hGH were 85.4°C and 88.6°C, respectively.

    Journal: Protein Engineering, Design and Selection

    Article Title: PASylation: a biological alternative to PEGylation for extending the plasma half-life of pharmaceutically active proteins

    doi: 10.1093/protein/gzt023

    Figure Lengend Snippet: Biophysical characterization of PAS#1(600)-hGH. ( A ) Mass spectrometric analysis of PAS#1(600)-hGH by ESI-MS, confirming the calculated molecular mass of 73121.65 Da as well as precisely monodisperse composition, without any indication of prematurely terminated gene products. ( B ) Hydrophobicity analysis of PASylated hGH by reverse-phase (RP) HPLC. HIC of the original recombinant hGH and its PASylated version was performed on a Resource RPC column using an elution gradient from 2% v/v acetonitrile, 0.065% v/v TFA to 80% v/v acetonitrile, 0.05% v/v TFA. Both profiles show a single homogeneous peak with slightly earlier elution of PAS#1(600)-hGH (51.8% acetonitrile) in comparison with the corresponding unfused protein (52.3% acetonitrile). ( C ) Thermal denaturation of hGH and PAS#1(600)-hGH as determined by far UV CD measurement. Thermal unfolding of a 8.2 µM protein solution in 50 mM K 2 SO 4 , 20 mM K-P i pH 7.5 was followed at a wavelength of 208 nm where maximal spectral change upon unfolding was observed and the normalized unfolded fraction, f(u), was plotted as a function of temperature. The melting temperatures ( T m ) of hGH and PAS#1(600)-hGH were 85.4°C and 88.6°C, respectively.

    Article Snippet: Reverse-Phase HPLC The purified protein solution in PBS was adjusted to 2% v/v acetonitrile, 0.065% v/v trifluoroacetic acid (TFA) and 1 ml was applied to a Resource RPC 1 ml column (GE Healthcare) equilibrated with buffer A (2% v/v acetonitrile, 0.065% v/v TFA).

    Techniques: Mass Spectrometry, High Performance Liquid Chromatography, Hydrophobic Interaction Chromatography, Recombinant

    Reverse-phase HPLC profile of lacticin Q produced by L. lactis QU 5. Lacticin Q was isolated using a 3-ml RESOURCE RPC column (Amersham Biosciences) at a flow rate of 1.0 ml/min. Antibacterial activity was detected in the fractions indicated by a double-headed

    Journal:

    Article Title: Structural Analysis and Characterization of Lacticin Q, a Novel Bacteriocin Belonging to a New Family of Unmodified Bacteriocins of Gram-Positive Bacteria ▿

    doi: 10.1128/AEM.02286-06

    Figure Lengend Snippet: Reverse-phase HPLC profile of lacticin Q produced by L. lactis QU 5. Lacticin Q was isolated using a 3-ml RESOURCE RPC column (Amersham Biosciences) at a flow rate of 1.0 ml/min. Antibacterial activity was detected in the fractions indicated by a double-headed

    Article Snippet: This fraction was applied to a 3-ml RESOURCE RPC column (Amersham Biosciences) in an LC-2000Plus high-performance liquid chromatography (HPLC) system (JASCO, Tokyo, Japan).

    Techniques: High Performance Liquid Chromatography, Produced, Isolation, Flow Cytometry, Activity Assay

    Purification of SAMP H1. (A) HiPrep SP cation-exchange chromatography of SepPac C 18 -concentrated skin mucus extract; (B) Mono S cation-exchange chromatography with urea of RPC-concentrated active fractions obtained upon HiPrep SP chromatography; (C) final

    Journal:

    Article Title: Proline Conformation-Dependent Antimicrobial Activity of a Proline-Rich Histone H1 N-Terminal Peptide Fragment Isolated from the Skin Mucus of Atlantic Salmon

    doi: 10.1128/AAC.49.6.2399-2406.2005

    Figure Lengend Snippet: Purification of SAMP H1. (A) HiPrep SP cation-exchange chromatography of SepPac C 18 -concentrated skin mucus extract; (B) Mono S cation-exchange chromatography with urea of RPC-concentrated active fractions obtained upon HiPrep SP chromatography; (C) final

    Article Snippet: Active fractions from the HiPrep SP chromatography were pooled and concentrated by binding to a Resource reversed-phase chromatography (RPC) column (Amersham Biosciences) equilibrated with 0.1% TFA.

    Techniques: Purification, Chromatography