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  • 99
    Thermo Fisher recombinant protein g agarose beads
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    Thermo Fisher pcdna3 0 vector
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    TaKaRa plasmid phcred1 1
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    New England Biolabs rabbit polyclonal anti mbp serum
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    TaKaRa plasmid pegfp
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    Thermo Fisher bglii
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    Cell Biolabs Inc paav mcs vector
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    Stratagene recombinant protein
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    Thermo Fisher pfastbac 1
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    Genethon plasmid puc57 rep2cap2
    Plasmid Puc57 Rep2cap2, supplied by Genethon, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene streptavidin binding peptide
    Characterization of the HeLaAAVtCR cell clone. (A) Schematic representation of the wt AAV-2 genome and of the AAVtCR derivative. In the AAVtCR construct the rep gene is fused at its N-terminus with a sequence coding for the calmodulin and <t>streptavidin</t> binding peptides (C and S) and the fluorescent mCherry protein. p5, p19, and p40 are the AAV promoters and the dotted line indicates the alternative splicing in the rep gene. The black box indicates the BGH polyA. (B) Synthesis of Rep proteins in HSV-1-infected HeLaAAVtCR and HA-16 cells. The cells were infected or not with wt HSV-1 at a multiplicity of infection (MOI) of 5 plaque forming units (pfu)/cell and analyzed for the synthesis of Rep proteins by Western blot. tCR indicates the tagCherryRep78 and 68 proteins. (C) Analysis of AVVtCR genome rescue and replication. Replication was assessed by Southern blot using a rep probe. mRF and dRF indicate the monomeric and dimeric replicative forms, respectively.
    Streptavidin Binding Peptide, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene calmodulin
    Characterization of the HeLaAAVtCR cell clone. (A) Schematic representation of the wt AAV-2 genome and of the AAVtCR derivative. In the AAVtCR construct the rep gene is fused at its N-terminus with a sequence coding for the <t>calmodulin</t> and streptavidin binding peptides (C and S) and the fluorescent mCherry protein. p5, p19, and p40 are the AAV promoters and the dotted line indicates the alternative splicing in the rep gene. The black box indicates the BGH polyA. (B) Synthesis of Rep proteins in HSV-1-infected HeLaAAVtCR and HA-16 cells. The cells were infected or not with wt HSV-1 at a multiplicity of infection (MOI) of 5 plaque forming units (pfu)/cell and analyzed for the synthesis of Rep proteins by Western blot. tCR indicates the tagCherryRep78 and 68 proteins. (C) Analysis of AVVtCR genome rescue and replication. Replication was assessed by Southern blot using a rep probe. mRF and dRF indicate the monomeric and dimeric replicative forms, respectively.
    Calmodulin, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Characterization of the HeLaAAVtCR cell clone. (A) Schematic representation of the wt AAV-2 genome and of the AAVtCR derivative. In the AAVtCR construct the rep gene is fused at its N-terminus with a sequence coding for the calmodulin and streptavidin binding peptides (C and S) and the fluorescent mCherry protein. p5, p19, and p40 are the AAV promoters and the dotted line indicates the alternative splicing in the rep gene. The black box indicates the BGH polyA. (B) Synthesis of Rep proteins in HSV-1-infected HeLaAAVtCR and HA-16 cells. The cells were infected or not with wt HSV-1 at a multiplicity of infection (MOI) of 5 plaque forming units (pfu)/cell and analyzed for the synthesis of Rep proteins by Western blot. tCR indicates the tagCherryRep78 and 68 proteins. (C) Analysis of AVVtCR genome rescue and replication. Replication was assessed by Southern blot using a rep probe. mRF and dRF indicate the monomeric and dimeric replicative forms, respectively.

    Journal: PLoS Pathogens

    Article Title: Definition of Herpes Simplex Virus Type 1 Helper Activities for Adeno-Associated Virus Early Replication Events

    doi: 10.1371/journal.ppat.1000340

    Figure Lengend Snippet: Characterization of the HeLaAAVtCR cell clone. (A) Schematic representation of the wt AAV-2 genome and of the AAVtCR derivative. In the AAVtCR construct the rep gene is fused at its N-terminus with a sequence coding for the calmodulin and streptavidin binding peptides (C and S) and the fluorescent mCherry protein. p5, p19, and p40 are the AAV promoters and the dotted line indicates the alternative splicing in the rep gene. The black box indicates the BGH polyA. (B) Synthesis of Rep proteins in HSV-1-infected HeLaAAVtCR and HA-16 cells. The cells were infected or not with wt HSV-1 at a multiplicity of infection (MOI) of 5 plaque forming units (pfu)/cell and analyzed for the synthesis of Rep proteins by Western blot. tCR indicates the tagCherryRep78 and 68 proteins. (C) Analysis of AVVtCR genome rescue and replication. Replication was assessed by Southern blot using a rep probe. mRF and dRF indicate the monomeric and dimeric replicative forms, respectively.

    Article Snippet: Plasmids The pAAVtCR plasmid ( ) was obtained by inserting the mCherry ORF from pRSETmCherry (provided by Y. Tsien) and the calmodulin and streptavidin binding peptide (CBP and SBP) tags from pNTAP-A (Interplay Mammalian TAP system, Stratagene) in a modified AAV-2 genome derived from the pSSV9 plasmid from which the cap gene had been removed and replaced with the Bovine Growth Hormone (BGH) polyadenylation site (polyA).

    Techniques: Construct, Sequencing, Binding Assay, Infection, Western Blot, Southern Blot

    Characterization of the HeLaAAVtCR cell clone. (A) Schematic representation of the wt AAV-2 genome and of the AAVtCR derivative. In the AAVtCR construct the rep gene is fused at its N-terminus with a sequence coding for the calmodulin and streptavidin binding peptides (C and S) and the fluorescent mCherry protein. p5, p19, and p40 are the AAV promoters and the dotted line indicates the alternative splicing in the rep gene. The black box indicates the BGH polyA. (B) Synthesis of Rep proteins in HSV-1-infected HeLaAAVtCR and HA-16 cells. The cells were infected or not with wt HSV-1 at a multiplicity of infection (MOI) of 5 plaque forming units (pfu)/cell and analyzed for the synthesis of Rep proteins by Western blot. tCR indicates the tagCherryRep78 and 68 proteins. (C) Analysis of AVVtCR genome rescue and replication. Replication was assessed by Southern blot using a rep probe. mRF and dRF indicate the monomeric and dimeric replicative forms, respectively.

    Journal: PLoS Pathogens

    Article Title: Definition of Herpes Simplex Virus Type 1 Helper Activities for Adeno-Associated Virus Early Replication Events

    doi: 10.1371/journal.ppat.1000340

    Figure Lengend Snippet: Characterization of the HeLaAAVtCR cell clone. (A) Schematic representation of the wt AAV-2 genome and of the AAVtCR derivative. In the AAVtCR construct the rep gene is fused at its N-terminus with a sequence coding for the calmodulin and streptavidin binding peptides (C and S) and the fluorescent mCherry protein. p5, p19, and p40 are the AAV promoters and the dotted line indicates the alternative splicing in the rep gene. The black box indicates the BGH polyA. (B) Synthesis of Rep proteins in HSV-1-infected HeLaAAVtCR and HA-16 cells. The cells were infected or not with wt HSV-1 at a multiplicity of infection (MOI) of 5 plaque forming units (pfu)/cell and analyzed for the synthesis of Rep proteins by Western blot. tCR indicates the tagCherryRep78 and 68 proteins. (C) Analysis of AVVtCR genome rescue and replication. Replication was assessed by Southern blot using a rep probe. mRF and dRF indicate the monomeric and dimeric replicative forms, respectively.

    Article Snippet: Plasmids The pAAVtCR plasmid ( ) was obtained by inserting the mCherry ORF from pRSETmCherry (provided by Y. Tsien) and the calmodulin and streptavidin binding peptide (CBP and SBP) tags from pNTAP-A (Interplay Mammalian TAP system, Stratagene) in a modified AAV-2 genome derived from the pSSV9 plasmid from which the cap gene had been removed and replaced with the Bovine Growth Hormone (BGH) polyadenylation site (polyA).

    Techniques: Construct, Sequencing, Binding Assay, Infection, Western Blot, Southern Blot