Journal: Scientific Reports
Article Title: Preventative effects of the partial RANKL peptide MHP1-AcN in a mouse model of imiquimod-induced psoriasis
Figure Lengend Snippet: Systemic administration of MHP1-AcN inhibited IMQ-induced IL-6, IL-23 and IL-17A expression. MHP1-AcN (100 µg/mouse) or saline was systemically administered by daily subcutaneous injection at a distant site from IMQ application. Mice were sacrificed at 32 h or d9 in two independent experiments. Dorsal skin samples where IMQ was applied and serum samples were collected. ( A ) The mRNA expression of Il6 , Il23 , and Il17a at 32 h and d9 in dorsal skin was analyzed by real-time PCR. ( B ) Serum IL-6, IL-23, and IL-17A levels at 32 h and d9 were measured by ELISA. *P
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) The concentrations of IL-6, IL-23, and IL-17A were measured using the following commercially available ELISA kits: IL-6, Mouse IL-6 Quantikine ELISA Kit (R & D Systems, Minneapolis, MN, USA); IL-23, Mouse IL-23 Quantikine ELISA Kit (R & D systems); and IL-17A, Mouse IL-17A Quantikine ELISA Kit (R & D Systems).
Techniques: Expressing, Injection, Mouse Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay