recombinant bovine il 13 Search Results


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  • 92
    Kingfisher Biotech recombinant bovine il 13
    Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) <t>IL-13</t> (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c
    Recombinant Bovine Il 13, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant bovine il 13/product/Kingfisher Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant bovine il 13 - by Bioz Stars, 2021-12
    92/100 stars
      Buy from Supplier

    93
    R&D Systems recombinant murine il 13
    Gene expression analysis of isolated NK populations. Relative fold expression in natural killer (NK)/natural killer T (NKT)-positive and -negative populations. mRNA transcript levels of interleukin 13 <t>(IL-13),</t> interferon-γ (IFN-γ), IL-17A, IL-17F, IL-22, IL-21, and IL-4 in freshly isolated NK positive (NK +) and negative (NK −) cells isolated cells from nonstressed (NS) and desiccating-stressed (DS) ocular surface (OS) for 5 (DS5) or 10 days (DS10) and normal spleen. *** P
    Recombinant Murine Il 13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant murine il 13/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant murine il 13 - by Bioz Stars, 2021-12
    93/100 stars
      Buy from Supplier

    Image Search Results


    Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: Fold changes in MDM production of nos2 ( a ) and arg1 ( b ) following stimulation with LPS (1000 ng/ml) IFNγ (20 ng/ml) or IL-4 (20 ng/ml) IL-13 (20 ng/ml) compared with changes in control cells, normalised with reference to transcription levels of GAPDH at 12 24 h. Fold changes in levels of nitric oxide in MDM cell supernatants from stimulated cells in comparison to unstimulated controls are shown in c . Supernatants and mRNA were harvested contemporaneously from the same cell populations and were performed in triplicate. Data from the same representative individual are shown in a , b and c

    Article Snippet: Cell stimulation MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques:

    Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Journal: BMC Veterinary Research

    Article Title: Stimulation of bovine monocyte-derived macrophages with lipopolysaccharide, interferon-ɣ, Interleukin-4 or Interleukin-13 does not induce detectable changes in nitric oxide or arginase activity

    doi: 10.1186/s12917-019-1785-0

    Figure Lengend Snippet: Levels of nitric oxide (1a 1b) and chitinase (1e 1f) in monocyte-derived and splenic macrophage cell supernatants and arginase (1c 1d) in cell lysates following stimulation with LPS and/or cytokines (LPS 1000 ng/ml, IFNɣ 20 ng/ml, IL-4 20 ng/ml and IL-13 20 ng/ml) for 40 h. Levels of enzyme product were determined by measuring OD values and comparison with standard curves. Means and standard errors of data from triplicate cultures from representative individual animals are shown, monocyte-derived macrophages all being obtained from one animal and splenic macrophages all from another individual. Similar findings were recorded from all individuals tested ( n = 5 MDM, n = 7 SM) for stimulation times of 12–72 h

    Article Snippet: Cell stimulation MDM or SM were stimulated by addition of 50–2000 ng/ml LPS from E. coli 0111:B4 (Insight Biotechnology Ltd., Middlesex) and/or 20-100 ng/ml bovine IFNγ (ThermoFisher Scientific, UK) or with 10-40 ng/ml recombinant bovine IL-4 (Kingfisher Biotech Inc., St Paul) and/or 10-20 ng/ml recombinant bovine IL-13 (Kingfisher Biotech Inc).

    Techniques: Derivative Assay

    Gene expression analysis of isolated NK populations. Relative fold expression in natural killer (NK)/natural killer T (NKT)-positive and -negative populations. mRNA transcript levels of interleukin 13 (IL-13), interferon-γ (IFN-γ), IL-17A, IL-17F, IL-22, IL-21, and IL-4 in freshly isolated NK positive (NK +) and negative (NK −) cells isolated cells from nonstressed (NS) and desiccating-stressed (DS) ocular surface (OS) for 5 (DS5) or 10 days (DS10) and normal spleen. *** P

    Journal: Mucosal immunology

    Article Title: Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13

    doi: 10.1038/mi.2010.82

    Figure Lengend Snippet: Gene expression analysis of isolated NK populations. Relative fold expression in natural killer (NK)/natural killer T (NKT)-positive and -negative populations. mRNA transcript levels of interleukin 13 (IL-13), interferon-γ (IFN-γ), IL-17A, IL-17F, IL-22, IL-21, and IL-4 in freshly isolated NK positive (NK +) and negative (NK −) cells isolated cells from nonstressed (NS) and desiccating-stressed (DS) ocular surface (OS) for 5 (DS5) or 10 days (DS10) and normal spleen. *** P

    Article Snippet: To evaluate if IL-13 could rescue DS-induced GC loss, C57BL/6 mice were divided into three treatment groups: (i) DS5 control subjects, which received no ocular injections; (ii) vehicle control animals, which received bilateral subconjunctival injections (20μl per eye) of 0.1% BSA in phosphate-buffered saline (DS5 + BSA); (iii) DS5 + IL-13 mice, which received daily bilateral subconjunctival injections of recombinant murine IL-13 (20 ng per eye per injection, dissolved in 20 μl of 0.1% BSA in phosphate-buffered saline (R & D Systems) for 4 days.

    Techniques: Expressing, Isolation

    NK and NKT cells produce IL-13. ( a ) Mean±s.d. of interleukin 13 (IL-13) enzyme-linked immunosorbent spots (ELISPOTs) after γδ + isolation from nonstressed (NS) ocular surface (OS) and NS spleen, showing the three isolated populations (αβ +, γδ +, and B220 and CD11b + cells). ( b ) Mean±s. d. of IL-13 ELISPOTs after natural killer (NK) isolation from NS and desiccated-stressed (DS) OS for 10 days (DS10) and NS spleen, showing the two isolated populations, NK positive ( + ) and NK negative ( − ). ( c ) Histogram of flow cytometry analysis of total splenocytes stained with NK1.1-phycoerythrin (PE)-conjugated antibody (gray line) or T-cell receptor αβ-antigen-presenting cell (TCRαβ-APC) and isotype control antibody (black line). The number over line indicates percentage of positive cells over the isotype control antibody. ( d, e ) Flow cytometry analysis. Lymphocytes were gated based on characteristic light-scatter properties, and single lymphocytes were gated based on forward scatter height vs. forward scatter area (FSC-A). The numbers in the quadrants or over line indicate the percentage of cells. ( d ) Histogram of flow cytometry analysis of NK-positive cells stained with NK1.1-PE-conjugated antibody (gray line) or isotype control antibody (black line). The number over line indicates percentage of positive cells over the isotype control antibody. ( e ) Flow cytometry analysis of freshly isolated NK cells dual stained with NK1.1-PE + TCRαβ in all populations (all cells=no fraction, NK +, and NK − cells) from NS spleens. Percentage of double-positive cells is indicated in top right quadrant. ( f ) Mean±s.d. of IL-13 ELISPOTs after CD45 isolation from NS OS.

    Journal: Mucosal immunology

    Article Title: Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13

    doi: 10.1038/mi.2010.82

    Figure Lengend Snippet: NK and NKT cells produce IL-13. ( a ) Mean±s.d. of interleukin 13 (IL-13) enzyme-linked immunosorbent spots (ELISPOTs) after γδ + isolation from nonstressed (NS) ocular surface (OS) and NS spleen, showing the three isolated populations (αβ +, γδ +, and B220 and CD11b + cells). ( b ) Mean±s. d. of IL-13 ELISPOTs after natural killer (NK) isolation from NS and desiccated-stressed (DS) OS for 10 days (DS10) and NS spleen, showing the two isolated populations, NK positive ( + ) and NK negative ( − ). ( c ) Histogram of flow cytometry analysis of total splenocytes stained with NK1.1-phycoerythrin (PE)-conjugated antibody (gray line) or T-cell receptor αβ-antigen-presenting cell (TCRαβ-APC) and isotype control antibody (black line). The number over line indicates percentage of positive cells over the isotype control antibody. ( d, e ) Flow cytometry analysis. Lymphocytes were gated based on characteristic light-scatter properties, and single lymphocytes were gated based on forward scatter height vs. forward scatter area (FSC-A). The numbers in the quadrants or over line indicate the percentage of cells. ( d ) Histogram of flow cytometry analysis of NK-positive cells stained with NK1.1-PE-conjugated antibody (gray line) or isotype control antibody (black line). The number over line indicates percentage of positive cells over the isotype control antibody. ( e ) Flow cytometry analysis of freshly isolated NK cells dual stained with NK1.1-PE + TCRαβ in all populations (all cells=no fraction, NK +, and NK − cells) from NS spleens. Percentage of double-positive cells is indicated in top right quadrant. ( f ) Mean±s.d. of IL-13 ELISPOTs after CD45 isolation from NS OS.

    Article Snippet: To evaluate if IL-13 could rescue DS-induced GC loss, C57BL/6 mice were divided into three treatment groups: (i) DS5 control subjects, which received no ocular injections; (ii) vehicle control animals, which received bilateral subconjunctival injections (20μl per eye) of 0.1% BSA in phosphate-buffered saline (DS5 + BSA); (iii) DS5 + IL-13 mice, which received daily bilateral subconjunctival injections of recombinant murine IL-13 (20 ng per eye per injection, dissolved in 20 μl of 0.1% BSA in phosphate-buffered saline (R & D Systems) for 4 days.

    Techniques: Isolation, Flow Cytometry, Cytometry, Staining

    Effects of CsA treatment on NK and NKT cells. ( a ) Mean±s.d. of conjunctival goblet cell (GC) density in nonstressed (NS) C57BL/6 control mice and mice subjected to desiccating stress for 5 or 10 days (DS5 and DS10, respectively) or DS5 treated with Cyclosporine A (DS5 + CsA) or DS5 treated with CsA vehicle (DS5 + Veh). ( b ) mRNA transcript levels of MUC5AC in conjunctiva of NS C57BL/6 control mice and mice subjected to DS5 and DS10, respectively. ( c ) Mean±.d. of protein concentrations of MUC5AC in conjunctiva of NS C57BL/6 control mice and mice subjected to DS5 and DS10, respectively. ( d, e ) Representative flow cytometry analysis of freshly isolated ocular surface cells stained with pan-NK marker DX5 –fluorescein isothiocyanate (FITC)-conjugated antibody in NS C57BL/6 controls mice and mice subjected to DS5 and DS10, respectively ( d ) or DS5 treated with CsA (DS5 + CsA) or DS5 treated with CsA vehicle (DS5 + Veh) ( e ). Lymphocytes were gated based on characteristic light-scatter properties, and single lymphocytes were gated based on forward scatter height vs. forward scatter area (FSC-A). Numbers in the quadrants indicate the percentage of cells. ( f ) Mean±s.d. of the percentage of DX5+ cells evaluated by flow cytometry in three independent experiments. ( g ) Percentage change in the level of expression of interferon-γ (IFN-γ), IL-13, IL-17A, IL-21, and IL-22 mRNA transcripts in CsA-treated NK + and NK − cell populations, compared with vehicle. *** P

    Journal: Mucosal immunology

    Article Title: Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13

    doi: 10.1038/mi.2010.82

    Figure Lengend Snippet: Effects of CsA treatment on NK and NKT cells. ( a ) Mean±s.d. of conjunctival goblet cell (GC) density in nonstressed (NS) C57BL/6 control mice and mice subjected to desiccating stress for 5 or 10 days (DS5 and DS10, respectively) or DS5 treated with Cyclosporine A (DS5 + CsA) or DS5 treated with CsA vehicle (DS5 + Veh). ( b ) mRNA transcript levels of MUC5AC in conjunctiva of NS C57BL/6 control mice and mice subjected to DS5 and DS10, respectively. ( c ) Mean±.d. of protein concentrations of MUC5AC in conjunctiva of NS C57BL/6 control mice and mice subjected to DS5 and DS10, respectively. ( d, e ) Representative flow cytometry analysis of freshly isolated ocular surface cells stained with pan-NK marker DX5 –fluorescein isothiocyanate (FITC)-conjugated antibody in NS C57BL/6 controls mice and mice subjected to DS5 and DS10, respectively ( d ) or DS5 treated with CsA (DS5 + CsA) or DS5 treated with CsA vehicle (DS5 + Veh) ( e ). Lymphocytes were gated based on characteristic light-scatter properties, and single lymphocytes were gated based on forward scatter height vs. forward scatter area (FSC-A). Numbers in the quadrants indicate the percentage of cells. ( f ) Mean±s.d. of the percentage of DX5+ cells evaluated by flow cytometry in three independent experiments. ( g ) Percentage change in the level of expression of interferon-γ (IFN-γ), IL-13, IL-17A, IL-21, and IL-22 mRNA transcripts in CsA-treated NK + and NK − cell populations, compared with vehicle. *** P

    Article Snippet: To evaluate if IL-13 could rescue DS-induced GC loss, C57BL/6 mice were divided into three treatment groups: (i) DS5 control subjects, which received no ocular injections; (ii) vehicle control animals, which received bilateral subconjunctival injections (20μl per eye) of 0.1% BSA in phosphate-buffered saline (DS5 + BSA); (iii) DS5 + IL-13 mice, which received daily bilateral subconjunctival injections of recombinant murine IL-13 (20 ng per eye per injection, dissolved in 20 μl of 0.1% BSA in phosphate-buffered saline (R & D Systems) for 4 days.

    Techniques: Mouse Assay, Flow Cytometry, Cytometry, Isolation, Staining, Marker, Expressing

    IL-13 expression and goblet cell analysis. ( a–c ) Immunohistochemical staining for interleukin 13 (IL-13; red, in a) and IL-13 receptor α1 (IL-13Rα1; red, in b) and goat anti-rabbit secondary antibody alone ( c ) in conjunctiva sections of nonstressed (NS) control mice. Scale bar = 25 μm. ( d ) mRNA transcript levels of IL-13 and interferon-γ (IFN-γ) in conjunctiva in NS control mice and mice subjected to desiccating stress for 5 or 10 days (DS5 and DS10, respectively). ( e ) Ratio of IL-13/IFN-γ mRNA transcripts in conjunctiva. ( f ) Mean±s.d. of goblet cell (GC) density in NS C57BL/6 (B6) control mice and mice subjected to DS5 and mice after subconjunctival injection of BSA (+ BSA) or IL-13 (+ IL13). ( g ) Mean±s.d. of GC density in NS C57BL/6 (B6) controls mice and mice subjected to DS5 and IL-13 knockout (KO) mice. ( h ) Mean±s.d. of GC density in NS Bal/c (BC) control mice and mice subjected to DS5 and DS10 and signal transducer and activator of transcription 6 knockout (STAT6KO) at baseline and after DS5. ( i ) Mean±s. d. of GC density in NS C57BL/6 mice (B6) that received systemic injection of neutralizing antibody (NK1.1) to natural killer (NK) cells (αNK) or isotype control (IC) antibody before NS and after DS5. ( j ) Mean±s.d. of GC density in NS C57BL/6 (B6) control and RAG1KO mice. ( k ) Mean±s.d. of GC density in NS Bal/c (BC) control mice and CD1dKO at baseline, NS, and after DS5. A separate group NS CD1dKO mice received systemic injection of depleting antibody (NK1.1) to NK cells (αNK) or IC antibody.

    Journal: Mucosal immunology

    Article Title: Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13

    doi: 10.1038/mi.2010.82

    Figure Lengend Snippet: IL-13 expression and goblet cell analysis. ( a–c ) Immunohistochemical staining for interleukin 13 (IL-13; red, in a) and IL-13 receptor α1 (IL-13Rα1; red, in b) and goat anti-rabbit secondary antibody alone ( c ) in conjunctiva sections of nonstressed (NS) control mice. Scale bar = 25 μm. ( d ) mRNA transcript levels of IL-13 and interferon-γ (IFN-γ) in conjunctiva in NS control mice and mice subjected to desiccating stress for 5 or 10 days (DS5 and DS10, respectively). ( e ) Ratio of IL-13/IFN-γ mRNA transcripts in conjunctiva. ( f ) Mean±s.d. of goblet cell (GC) density in NS C57BL/6 (B6) control mice and mice subjected to DS5 and mice after subconjunctival injection of BSA (+ BSA) or IL-13 (+ IL13). ( g ) Mean±s.d. of GC density in NS C57BL/6 (B6) controls mice and mice subjected to DS5 and IL-13 knockout (KO) mice. ( h ) Mean±s.d. of GC density in NS Bal/c (BC) control mice and mice subjected to DS5 and DS10 and signal transducer and activator of transcription 6 knockout (STAT6KO) at baseline and after DS5. ( i ) Mean±s. d. of GC density in NS C57BL/6 mice (B6) that received systemic injection of neutralizing antibody (NK1.1) to natural killer (NK) cells (αNK) or isotype control (IC) antibody before NS and after DS5. ( j ) Mean±s.d. of GC density in NS C57BL/6 (B6) control and RAG1KO mice. ( k ) Mean±s.d. of GC density in NS Bal/c (BC) control mice and CD1dKO at baseline, NS, and after DS5. A separate group NS CD1dKO mice received systemic injection of depleting antibody (NK1.1) to NK cells (αNK) or IC antibody.

    Article Snippet: To evaluate if IL-13 could rescue DS-induced GC loss, C57BL/6 mice were divided into three treatment groups: (i) DS5 control subjects, which received no ocular injections; (ii) vehicle control animals, which received bilateral subconjunctival injections (20μl per eye) of 0.1% BSA in phosphate-buffered saline (DS5 + BSA); (iii) DS5 + IL-13 mice, which received daily bilateral subconjunctival injections of recombinant murine IL-13 (20 ng per eye per injection, dissolved in 20 μl of 0.1% BSA in phosphate-buffered saline (R & D Systems) for 4 days.

    Techniques: Expressing, Immunohistochemistry, Staining, Mouse Assay, Injection, Knock-Out