Journal: Experimental and Therapeutic Medicine
Article Title: Effect of miRNA-19a on gastrointestinal motility in rats with functional dyspepsia
Figure Lengend Snippet: Comparison of difference of miRNA-19a expression between model and normal group. The contents of miRNA-19a in rat gastric tissue of model and normal group are detected by qRT-PCR, which displayed that the relative content of miRNA-19a in gastric tissue of model group is 6.13±0.89, which is 4.56±0.65 in normal tissue, suggesting that miRNA-19a is highly expressed in rats with functional dyspepsia, *P
Article Snippet: TRIzol, reverse-transcription kit and real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) kit (Promega Corp., Madison, WI, USA), miRNA-19a, U6 specific primers (Shanghai Sangon Biological Engineering Co., Ltd., Shanghai, China), miRNA-19a sequence mimics (miRNA-19a mimic) and miRNA-19a unrelated sequence mimics (scramble mimic) (Shanghai Sangon Biological Engineering Co., Ltd.), In vivo -jetPEI® (Polyplus Transfection, Illkirch, France), rat motilin (MTL), vasoactive intestinal peptide (VIP) (Wuhan Huamei Biological Engineering Co., Ltd., Wuhan, China), real-time fluorescent quantitative PCR 7500 system (Applied Biosystems, Foster City, CA, USA).
Techniques: Expressing, Quantitative RT-PCR, Functional Assay