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  • 99
    Thermo Fisher real time pcr stepone real time pcr system
    Real <t>time-PCR</t> for GSK3-β; Total RNA was extracted; the standard reverse transcription was performed using RevertAid First Strand cDNA Synthesis Kit. Subsequent real time PCR was done with Power SYBR Green real time master mix and <t>Stepone</t> real time PCR (Applied Biosystems, Carlsbad, U.S.A.). GAPDH primer was used as a housekeeping gene. Each reaction was performed in triplicate. Expression of GSK3-β was down-regulated in non-obstructive azoospermia (3.10±0.19) compared with normal (7.12±0.39) and obstructive azoospermia (6.32±0.42) groups. The difference was significant (p=0.001)
    Real Time Pcr Stepone Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bruker Corporation species specific real time pcr
    Real <t>time-PCR</t> for GSK3-β; Total RNA was extracted; the standard reverse transcription was performed using RevertAid First Strand cDNA Synthesis Kit. Subsequent real time PCR was done with Power SYBR Green real time master mix and <t>Stepone</t> real time PCR (Applied Biosystems, Carlsbad, U.S.A.). GAPDH primer was used as a housekeeping gene. Each reaction was performed in triplicate. Expression of GSK3-β was down-regulated in non-obstructive azoospermia (3.10±0.19) compared with normal (7.12±0.39) and obstructive azoospermia (6.32±0.42) groups. The difference was significant (p=0.001)
    Species Specific Real Time Pcr, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/species specific real time pcr/product/Bruker Corporation
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher real time pcr real time pcr
    miR-16, let-7a and miR-34a are increased during mouse ES cell differentiation . Mouse ES cells (Sox1-GFP 46C) were differentiated using an adherent monolayer protocol. Cells at 4 and 8 days were collected for total RNA extraction and subsequently processed for evaluation of specific differentiation markers, as well as proapoptotic miRNA expression by quantitative Real <t>Time-PCR.</t> A positive control for neural differentiation was also performed at day 8 by treating cells with either 10 nM LY411575 or 0.01%DMSO (control) for 12 hours. A) Semi-quantitative RT-PCR analysis for selected markers of lineage commitment in day 4 and 8, as well as in LY411575-treated and untreated cells. B) Representative bright-field, phase contrast images showing increased neuronal differentiation after LY411575 incubation compared with control (DMSO-treated) cells. C) Expression of miR-16, Let-7a and miR-34a at 4 and 8 days of ES cell differentiation and in control (DMSO-treated) and LY411575-treated rosette cultures at 8 days. <t>miRNAs</t> expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA, and GAPDH for normalization. Expression levels were calculated by the ΔΔ C t method using either differentiated cells at 4 days or LY411575-untreated cells as calibrator. Data represent mean ± SEM of three independent experiments. * p
    Real Time Pcr Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 912 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher realtime pcr
    STING mutants fail to respond to DNA-adduct forming agents. The reconstituted primary Sting −/− MEF cells with empty vector (pBabe), hSTING, R169W, or P203S were treated DMBA (20 μg/ml) or cisplatin (10 μM) for 48 hr. Total RNA was extracted and <t>realtime</t> <t>PCR</t> was performed with the indicated probes after cDNA synthesis. Data shown here are the averages ± SD (n = 3). Asterisks indicate significant difference (p
    Realtime Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher real time pcr rq pcr
    a Specificity of real-time <t>PCR</t> amplification melting temperature analysis of PCR products recorded in ABI <t>7500</t> Real-time PCR system (Applied Biosystems, Foster City, CA). Descriptions: NC - DNA extracted from non infected SPF chicken kidneys (CEKs). b Specificity NC - DNA extracted from non infected SPF chicken kidneys (CEKs). No real-time PCR curve was observed in the case of the negative control, nor for DNA obtained from other viruses: CAV, HEV, DAdV. Rel-time PCR curve was observed in the case of FAdV infection
    Real Time Pcr Rq Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher real time pcr quantitative real time pcr
    a Specificity of real-time <t>PCR</t> amplification melting temperature analysis of PCR products recorded in ABI <t>7500</t> Real-time PCR system (Applied Biosystems, Foster City, CA). Descriptions: NC - DNA extracted from non infected SPF chicken kidneys (CEKs). b Specificity NC - DNA extracted from non infected SPF chicken kidneys (CEKs). No real-time PCR curve was observed in the case of the negative control, nor for DNA obtained from other viruses: CAV, HEV, DAdV. Rel-time PCR curve was observed in the case of FAdV infection
    Real Time Pcr Quantitative Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher real time pcr amplification
    Expression of oppA mRNA transcripts in cultured B. burgdorferi . Expression of the oppA genes was measured by RT <t>PCR</t> (A) and by RPA (B). For RT PCR, RNA was isolated from B. burgdorferi grown in BSK H medium at 37°C. <t>cDNA</t> was generated from total RNA, using gene-specific primers. For each sample, quantitative RT PCR for oppA-I to -V was performed. Determination of copy numbers was calculated by comparison to individual standard curves generated for each primer set. Panel A shows the results of three separate experiments. For RPA, total RNA was hybridized to biotinylated probes specific for each oppA gene and then digested with RNase A-RNase T 1 . Samples were subjected to electrophoresis in a polyacrylamide gel and transferred to nylon membranes. Detection of biotinylated probes was done using chemiluminescence, and quantitation was performed by scanning densitometry. Data shown are individual results from three separate experiments.
    Real Time Pcr Amplification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4779 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Eppendorf AG real time pcr analysis
    mRNA and protein expression for AR in TG of male and female rats. (A) <t>PCR</t> of TG <t>cDNA</t> produced a single band of the expected size for AR (278 bp, A) in male and female TG. M, marker, NTS, no template control. (B) Bar graphs showing quantitative comparisons
    Real Time Pcr Analysis, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher real time pcr machine
    The amplification plot of <t>FMDV</t> isolates with classical <t>rRT-PCR</t> reagents and AuNPs-FMDV biosensor: the amplification plot is showing amplification of FMDV isolates with classical rRT-PCR reagents (yellow and violet) curves with CT values 7.03 and 15.9 and with AuNPs-FMDV biosensor (red and blue) curves with CT values 4.09 and 12.93
    Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher real time pcr system
    Effect of <t>miR-31</t> on cisplatin sensitivity and invasion capacity of DDP-resistant cells A. The affirmation of the level of miR-31 mRNA expressed in GBC-SD/DDP and NOZ/DDP cells transfected with miR-31 mimic by <t>qRT-PCR.</t> B. The cell viability plotted against the concentration of DDP in GBC-SD/DPP and NOZ/DPP cells transfected with miR-31 or vector. C. Colony formation assay of DDP-resistant cells transfected with miR-31 or vector after exposure to DDP. D. The DDP-induced apoptosis rate of GBC-SD/DPP and NOZ/DPP cells transfected with miR-31 or vector analyzed by flow cytometry. E. The invasive ability of DDP-resistant cells transfected with miR-31 or vector after treatment with DDP in the transwell invasion assay. Data were presented as mean ± SD. * P
    Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Real time-PCR for GSK3-β; Total RNA was extracted; the standard reverse transcription was performed using RevertAid First Strand cDNA Synthesis Kit. Subsequent real time PCR was done with Power SYBR Green real time master mix and Stepone real time PCR (Applied Biosystems, Carlsbad, U.S.A.). GAPDH primer was used as a housekeeping gene. Each reaction was performed in triplicate. Expression of GSK3-β was down-regulated in non-obstructive azoospermia (3.10±0.19) compared with normal (7.12±0.39) and obstructive azoospermia (6.32±0.42) groups. The difference was significant (p=0.001)

    Journal: Iranian Journal of Reproductive Medicine

    Article Title: Expression of Glycogen synthase kinase 3-? (GSK3-?) gene in azoospermic men

    doi:

    Figure Lengend Snippet: Real time-PCR for GSK3-β; Total RNA was extracted; the standard reverse transcription was performed using RevertAid First Strand cDNA Synthesis Kit. Subsequent real time PCR was done with Power SYBR Green real time master mix and Stepone real time PCR (Applied Biosystems, Carlsbad, U.S.A.). GAPDH primer was used as a housekeeping gene. Each reaction was performed in triplicate. Expression of GSK3-β was down-regulated in non-obstructive azoospermia (3.10±0.19) compared with normal (7.12±0.39) and obstructive azoospermia (6.32±0.42) groups. The difference was significant (p=0.001)

    Article Snippet: Subsequent real time PCR was done with Power Syber Green real time master mix and Stepone real time PCR (Applied biosystems, Carlsbad, USA).

    Techniques: Real-time Polymerase Chain Reaction, SYBR Green Assay, Expressing

    miR-16, let-7a and miR-34a are increased during mouse ES cell differentiation . Mouse ES cells (Sox1-GFP 46C) were differentiated using an adherent monolayer protocol. Cells at 4 and 8 days were collected for total RNA extraction and subsequently processed for evaluation of specific differentiation markers, as well as proapoptotic miRNA expression by quantitative Real Time-PCR. A positive control for neural differentiation was also performed at day 8 by treating cells with either 10 nM LY411575 or 0.01%DMSO (control) for 12 hours. A) Semi-quantitative RT-PCR analysis for selected markers of lineage commitment in day 4 and 8, as well as in LY411575-treated and untreated cells. B) Representative bright-field, phase contrast images showing increased neuronal differentiation after LY411575 incubation compared with control (DMSO-treated) cells. C) Expression of miR-16, Let-7a and miR-34a at 4 and 8 days of ES cell differentiation and in control (DMSO-treated) and LY411575-treated rosette cultures at 8 days. miRNAs expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA, and GAPDH for normalization. Expression levels were calculated by the ΔΔ C t method using either differentiated cells at 4 days or LY411575-untreated cells as calibrator. Data represent mean ± SEM of three independent experiments. * p

    Journal: BMC Genomics

    Article Title: Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation

    doi: 10.1186/1471-2164-11-514

    Figure Lengend Snippet: miR-16, let-7a and miR-34a are increased during mouse ES cell differentiation . Mouse ES cells (Sox1-GFP 46C) were differentiated using an adherent monolayer protocol. Cells at 4 and 8 days were collected for total RNA extraction and subsequently processed for evaluation of specific differentiation markers, as well as proapoptotic miRNA expression by quantitative Real Time-PCR. A positive control for neural differentiation was also performed at day 8 by treating cells with either 10 nM LY411575 or 0.01%DMSO (control) for 12 hours. A) Semi-quantitative RT-PCR analysis for selected markers of lineage commitment in day 4 and 8, as well as in LY411575-treated and untreated cells. B) Representative bright-field, phase contrast images showing increased neuronal differentiation after LY411575 incubation compared with control (DMSO-treated) cells. C) Expression of miR-16, Let-7a and miR-34a at 4 and 8 days of ES cell differentiation and in control (DMSO-treated) and LY411575-treated rosette cultures at 8 days. miRNAs expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA, and GAPDH for normalization. Expression levels were calculated by the ΔΔ C t method using either differentiated cells at 4 days or LY411575-untreated cells as calibrator. Data represent mean ± SEM of three independent experiments. * p

    Article Snippet: Evaluation of miRNAs expression levels by quantitative Real Time-PCR Real-time PCR was performed in an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Techniques: Cell Differentiation, RNA Extraction, Expressing, Real-time Polymerase Chain Reaction, Positive Control, Quantitative RT-PCR, Incubation

    Apoptosis-associated miRNAs are modulated during mouse NS cell differentiation . Expression of specific proapoptotic (miR-16, let-7a and miR-34a) and antiapoptotic miRNAs (miR-20a and miR-19a) were analyzed by quantitative Real Time-PCR from 10 ng of total RNA using specific Taqman primers and GAPDH for normalization. * p

    Journal: BMC Genomics

    Article Title: Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation

    doi: 10.1186/1471-2164-11-514

    Figure Lengend Snippet: Apoptosis-associated miRNAs are modulated during mouse NS cell differentiation . Expression of specific proapoptotic (miR-16, let-7a and miR-34a) and antiapoptotic miRNAs (miR-20a and miR-19a) were analyzed by quantitative Real Time-PCR from 10 ng of total RNA using specific Taqman primers and GAPDH for normalization. * p

    Article Snippet: Evaluation of miRNAs expression levels by quantitative Real Time-PCR Real-time PCR was performed in an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Techniques: Cell Differentiation, Expressing, Real-time Polymerase Chain Reaction

    Inhibition of apoptosis by TUDCA was not associated with a decrease in proapoptotic miRNAs expression . Mouse NS cells with 3 days of differentiation were either untreated or treated with 50 μM of TUDCA for 72 hours. Collected cells were stained with Annexin-V-APC/PI to evaluate cell death, or processed for total RNA extraction and miRNAs expression evaluation by quantitative Real Time-PCR. A) Representative Annexin V-APC/PI stainings showing decreased cell death after TUDCA incubation. B) Quantitation of either dying (Annexin+/PI-) or dead (Annexin+/PI+) cells depicted in FACS diagrams. Results are mean ± SEM of triplicates. C) Expression of proapoptotic miRNAs at 3 and 6 days, with or without TUDCA treatment. miR-16, let-7a and miR-34a expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA, and GAPDH for normalization. Expression levels were calculated by the ΔΔ C t method using differentiated cells at 3 days as calibrator. Data represent mean ± SEM of three independent experiments. * p

    Journal: BMC Genomics

    Article Title: Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation

    doi: 10.1186/1471-2164-11-514

    Figure Lengend Snippet: Inhibition of apoptosis by TUDCA was not associated with a decrease in proapoptotic miRNAs expression . Mouse NS cells with 3 days of differentiation were either untreated or treated with 50 μM of TUDCA for 72 hours. Collected cells were stained with Annexin-V-APC/PI to evaluate cell death, or processed for total RNA extraction and miRNAs expression evaluation by quantitative Real Time-PCR. A) Representative Annexin V-APC/PI stainings showing decreased cell death after TUDCA incubation. B) Quantitation of either dying (Annexin+/PI-) or dead (Annexin+/PI+) cells depicted in FACS diagrams. Results are mean ± SEM of triplicates. C) Expression of proapoptotic miRNAs at 3 and 6 days, with or without TUDCA treatment. miR-16, let-7a and miR-34a expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA, and GAPDH for normalization. Expression levels were calculated by the ΔΔ C t method using differentiated cells at 3 days as calibrator. Data represent mean ± SEM of three independent experiments. * p

    Article Snippet: Evaluation of miRNAs expression levels by quantitative Real Time-PCR Real-time PCR was performed in an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Techniques: Inhibition, Expressing, Staining, RNA Extraction, Real-time Polymerase Chain Reaction, Incubation, Quantitation Assay, FACS

    Differentiation of PC12 and NT2N cells were associated with modulated levels of miR-16, let-7a and miR-34a expression . PC12 cells were differentiated upon NGF treatment at 1 day with either 5 or 50 ng/ml. NGF-untreated cells were also prepared. Cells were collected at 1, 2 4 and 7 days. NT2N cells were induced to differentiate with 10 μM all-trans retinoic acid and collected at 0, 14 and 21 days. In parallel experiments, cells were replated at 14 days in Matrigel, deprived of retinoic acid and incubated with mitotic inhibitors and further cultured for 7 days. Control cells were maintained in initial retinoic acid treatment. Both PC12 and NT2N-collected cells were processed for total RNA extraction and evaluation of miRNAs expression levels using quantitative Real Time-PCR. A and B) miR-16, let-7a and miR-34a expression during PC12 and NT2N differentiation, respectively. miRNAs expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA. GAPDH and RNU6B were used for normalization in PC12 and NT2N cells, respectively. Expression levels were calculated by the ΔΔ C t method. NGF-untreated cells at 1 day was used as calibrator in PC12 cells, while undifferentiated (day 0) or unreplated cells were used as calibrator in NT2N cells. Data represent mean ± SEM of three independent experiments. A) * p

    Journal: BMC Genomics

    Article Title: Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation

    doi: 10.1186/1471-2164-11-514

    Figure Lengend Snippet: Differentiation of PC12 and NT2N cells were associated with modulated levels of miR-16, let-7a and miR-34a expression . PC12 cells were differentiated upon NGF treatment at 1 day with either 5 or 50 ng/ml. NGF-untreated cells were also prepared. Cells were collected at 1, 2 4 and 7 days. NT2N cells were induced to differentiate with 10 μM all-trans retinoic acid and collected at 0, 14 and 21 days. In parallel experiments, cells were replated at 14 days in Matrigel, deprived of retinoic acid and incubated with mitotic inhibitors and further cultured for 7 days. Control cells were maintained in initial retinoic acid treatment. Both PC12 and NT2N-collected cells were processed for total RNA extraction and evaluation of miRNAs expression levels using quantitative Real Time-PCR. A and B) miR-16, let-7a and miR-34a expression during PC12 and NT2N differentiation, respectively. miRNAs expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA. GAPDH and RNU6B were used for normalization in PC12 and NT2N cells, respectively. Expression levels were calculated by the ΔΔ C t method. NGF-untreated cells at 1 day was used as calibrator in PC12 cells, while undifferentiated (day 0) or unreplated cells were used as calibrator in NT2N cells. Data represent mean ± SEM of three independent experiments. A) * p

    Article Snippet: Evaluation of miRNAs expression levels by quantitative Real Time-PCR Real-time PCR was performed in an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Techniques: Expressing, Incubation, Cell Culture, RNA Extraction, Real-time Polymerase Chain Reaction

    STING mutants fail to respond to DNA-adduct forming agents. The reconstituted primary Sting −/− MEF cells with empty vector (pBabe), hSTING, R169W, or P203S were treated DMBA (20 μg/ml) or cisplatin (10 μM) for 48 hr. Total RNA was extracted and realtime PCR was performed with the indicated probes after cDNA synthesis. Data shown here are the averages ± SD (n = 3). Asterisks indicate significant difference (p

    Journal: Oncogene

    Article Title: Suppression of STING Signaling through Epigenetic Silencing and Missense Mutation Impedes DNA-Damage Mediated Cytokine Production

    doi: 10.1038/s41388-017-0120-0

    Figure Lengend Snippet: STING mutants fail to respond to DNA-adduct forming agents. The reconstituted primary Sting −/− MEF cells with empty vector (pBabe), hSTING, R169W, or P203S were treated DMBA (20 μg/ml) or cisplatin (10 μM) for 48 hr. Total RNA was extracted and realtime PCR was performed with the indicated probes after cDNA synthesis. Data shown here are the averages ± SD (n = 3). Asterisks indicate significant difference (p

    Article Snippet: Realtime PCR was performed using StepOnePlus Real-Time PCR system (Applied Biosystems).

    Techniques: Plasmid Preparation, Polymerase Chain Reaction

    Glutamate-induced ERK activation stimulates the expression of both HSP70 and VRK3 to suppress its sustained activity that causes cell death. ( a ) The protein levels of HSP70 and VRK3 were positively correlated with ERK activity. ( b ) Blocking ERK phosphorylation using PD98059, a specific MEK/ERK inhibitor, delayed an increase in both HSP70 and VRK3 protein upon glutamate exposure. ( c , d ) Inhibition of ERK activation delayed mRNA expression of HSP70 ( c ) and VRK3 ( d ) following glutamate treatment. The mRNA levels of endogenous genes were detected by quantitative real-time PCR. Values are normalized to RPL32 and shown as mean ± s.d., n = 3. Student’s t-tests, **P

    Journal: Scientific Reports

    Article Title: VRK3-mediated nuclear localization of HSP70 prevents glutamate excitotoxicity-induced apoptosis and Aβ accumulation via enhancement of ERK phosphatase VHR activity

    doi: 10.1038/srep38452

    Figure Lengend Snippet: Glutamate-induced ERK activation stimulates the expression of both HSP70 and VRK3 to suppress its sustained activity that causes cell death. ( a ) The protein levels of HSP70 and VRK3 were positively correlated with ERK activity. ( b ) Blocking ERK phosphorylation using PD98059, a specific MEK/ERK inhibitor, delayed an increase in both HSP70 and VRK3 protein upon glutamate exposure. ( c , d ) Inhibition of ERK activation delayed mRNA expression of HSP70 ( c ) and VRK3 ( d ) following glutamate treatment. The mRNA levels of endogenous genes were detected by quantitative real-time PCR. Values are normalized to RPL32 and shown as mean ± s.d., n = 3. Student’s t-tests, **P

    Article Snippet: Quantitative real-time PCR The mRNA levels of endogenous genes were detected by quantitative real-time PCR using a StepOnePlus Real-Time PCR System (Applied Biosystems) with the FastStart Universal SYBR Green Master (Roche Applied Science).

    Techniques: Activation Assay, Expressing, Activity Assay, Blocking Assay, Inhibition, Real-time Polymerase Chain Reaction

    miR-21 is overexpressed in high-grade intraepithelial neoplasia (HG-IEN) and Barrett's adenocarcinoma (BAc). (A) Predicted base complementarity of miR-21 to 3′-UTR binding site of PDCD4 , as predicted by the Sanger miR-database (miRBase, http://www.mirbase.org/ ). (B) Altered miR-21 expression between cancerous tissue (CT; HG-IEN and BAc) and non-cancerous tissue (NCT) in BAc patients by qRT-PCR analysis. miR-21 was significantly upregulated in HG-IEN and BAc samples (p

    Journal: Journal of Clinical Pathology

    Article Title: Programmed cell death 4 (PDCD4) expression during multistep Barrett's carcinogenesis

    doi: 10.1136/jcp.2010.078253

    Figure Lengend Snippet: miR-21 is overexpressed in high-grade intraepithelial neoplasia (HG-IEN) and Barrett's adenocarcinoma (BAc). (A) Predicted base complementarity of miR-21 to 3′-UTR binding site of PDCD4 , as predicted by the Sanger miR-database (miRBase, http://www.mirbase.org/ ). (B) Altered miR-21 expression between cancerous tissue (CT; HG-IEN and BAc) and non-cancerous tissue (NCT) in BAc patients by qRT-PCR analysis. miR-21 was significantly upregulated in HG-IEN and BAc samples (p

    Article Snippet: The NCodeTM miRNA qRT-PCR method (Invitrogen, Carlsbad, California, USA) was used to detect and quantify mature miR-21 (primer sequence: 5′-CGGTAGCTTATCAGACTGATGTTGA-3′) on real-time PCR instruments according to the manufacturer's instructions (Applied Biosystems, Foster City, California, USA).

    Techniques: BAC Assay, Binding Assay, Expressing, Quantitative RT-PCR

    Involvement of NF-κB and STAT3 signaling pathways in IEC–adipocyte inflammatory interactions. (a) Mouse IECs were cultured for 2 days in medium from MEF-derived adipocytes (Ad sup) or normal medium (–). Relative MMP-9 mRNA levels were determined by quantitative RT-PCR normalized to GAPDH mRNA levels. The assay was performed in triplicate. Data are presented as mean ± SEM, * P

    Journal: EBioMedicine

    Article Title: Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells

    doi: 10.1016/j.ebiom.2017.07.027

    Figure Lengend Snippet: Involvement of NF-κB and STAT3 signaling pathways in IEC–adipocyte inflammatory interactions. (a) Mouse IECs were cultured for 2 days in medium from MEF-derived adipocytes (Ad sup) or normal medium (–). Relative MMP-9 mRNA levels were determined by quantitative RT-PCR normalized to GAPDH mRNA levels. The assay was performed in triplicate. Data are presented as mean ± SEM, * P

    Article Snippet: Reverse transcription was then performed using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems), and mRNA levels were quantified by fluorescence real-time PCR on a StepOnePlus (Applied Biosystems) using TaqMan Gene Expression Assays (Applied Biosystems) or PrimeTime qPCR Assays (Integrated DNA Technologies).

    Techniques: Cell Culture, Derivative Assay, Quantitative RT-PCR

    Inflammatory induction in adipocytes by co-culturing with monolayer IECs. (a) After co-cultured in pre-stimulation medium for 2 days with mouse IECs, MEF adipocytes (Day 9; MEF Ad) were harvested, and the relative mRNA levels were determined by quantitative RT-PCR and normalized to 18 s rRNA levels. The assay was performed in triplicate. Data are presented as mean ± SEM, * P

    Journal: EBioMedicine

    Article Title: Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells

    doi: 10.1016/j.ebiom.2017.07.027

    Figure Lengend Snippet: Inflammatory induction in adipocytes by co-culturing with monolayer IECs. (a) After co-cultured in pre-stimulation medium for 2 days with mouse IECs, MEF adipocytes (Day 9; MEF Ad) were harvested, and the relative mRNA levels were determined by quantitative RT-PCR and normalized to 18 s rRNA levels. The assay was performed in triplicate. Data are presented as mean ± SEM, * P

    Article Snippet: Reverse transcription was then performed using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems), and mRNA levels were quantified by fluorescence real-time PCR on a StepOnePlus (Applied Biosystems) using TaqMan Gene Expression Assays (Applied Biosystems) or PrimeTime qPCR Assays (Integrated DNA Technologies).

    Techniques: Cell Culture, Quantitative RT-PCR

    Generation and characterization of monolayer IECs from human iPSC-derived intestinal organoids. (a) TER values of the human organoid-derived cells that were broken by a 29G needle, seeded in collagen-coated Transwells with human organoid culture medium, were measured on the indicated days. The assay was performed in triplicate. Data are presented as mean ± SEM of one representative of three independent experiments. (b) Immunohistochemistry analysis of frozen sections of human organoid-derived cells described in (a) stained with anti-E-cadherin (green) and anti-villin1 (red) antibodies, and counterstained with DAPI (blue). The right image is a magnification of the boxed area. Data are representative of three independent experiments. A scale bar, 20 μm. (c) Whole-mount images of the human organoid-derived cells described in (a) stained with DAPI (blue), together with each antibody toward the indicated molecules. Arrowheads indicate each positive cell. Scale bars, 50 μm. (d–f) Human organoid-derived cells were cultured with human organoid culture medium for 6–8 days and then exposed to pre-stimulation medium for 1 day, followed by medium containing the indicated concentrations of recombinant cytokines for 2 days. Relative pIgR, Reg3β, and villin1 mRNA levels were determined by quantitative RT-PCR and normalized to GAPDH mRNA levels. The assay was performed in triplicate. Data are presented as mean ± SEM of one experiment representative of at least two independent experiments.

    Journal: EBioMedicine

    Article Title: Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells

    doi: 10.1016/j.ebiom.2017.07.027

    Figure Lengend Snippet: Generation and characterization of monolayer IECs from human iPSC-derived intestinal organoids. (a) TER values of the human organoid-derived cells that were broken by a 29G needle, seeded in collagen-coated Transwells with human organoid culture medium, were measured on the indicated days. The assay was performed in triplicate. Data are presented as mean ± SEM of one representative of three independent experiments. (b) Immunohistochemistry analysis of frozen sections of human organoid-derived cells described in (a) stained with anti-E-cadherin (green) and anti-villin1 (red) antibodies, and counterstained with DAPI (blue). The right image is a magnification of the boxed area. Data are representative of three independent experiments. A scale bar, 20 μm. (c) Whole-mount images of the human organoid-derived cells described in (a) stained with DAPI (blue), together with each antibody toward the indicated molecules. Arrowheads indicate each positive cell. Scale bars, 50 μm. (d–f) Human organoid-derived cells were cultured with human organoid culture medium for 6–8 days and then exposed to pre-stimulation medium for 1 day, followed by medium containing the indicated concentrations of recombinant cytokines for 2 days. Relative pIgR, Reg3β, and villin1 mRNA levels were determined by quantitative RT-PCR and normalized to GAPDH mRNA levels. The assay was performed in triplicate. Data are presented as mean ± SEM of one experiment representative of at least two independent experiments.

    Article Snippet: Reverse transcription was then performed using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems), and mRNA levels were quantified by fluorescence real-time PCR on a StepOnePlus (Applied Biosystems) using TaqMan Gene Expression Assays (Applied Biosystems) or PrimeTime qPCR Assays (Integrated DNA Technologies).

    Techniques: Derivative Assay, Immunohistochemistry, Staining, Cell Culture, Recombinant, Quantitative RT-PCR

    Generation and characterization of mouse monolayer IECs from primary small intestinal organoids. (a) Mouse organoid-derived cells were broken by a 26G needle and seeded in type I collagen-coated Transwells. After they were cultured with mouse organoid culture medium supplemented with 300 ng/mL recombinant mWnt3a, TER values were measured on the indicated days in quadruplicate. Data are presented as mean ± SEM of one experiment representative of three independent experiments. (b) Immunohistochemistry analysis of the monolayer harvested with the Transwell membrane at 12 days of culture. Sections were stained with anti-E-cadherin (green) and anti-villin1 (red) antibodies, and counterstained with DAPI (blue). The right image is a magnification of the boxed region. Data are representative of three independent experiments. Scale bar, 20 μm. (c) Whole-mount images of the cells visualized by confocal laser microscopy after fixing and staining with antibodies toward the indicated molecules. Arrowheads indicate each positive cell. Data are representative of two independent experiments. Scale bars, 50 μm. (d–f) Mouse monolayer IECs that had been cultured for 13 days in Transwells and then replaced by pre-stimulation medium to starve cytokines for 1 day, followed by the stimulation with various concentrations of the indicated cytokines for 2 days. The relative mRNA level was determined by quantitative RT-PCR and normalized to GAPDH mRNA levels. The assay was performed in triplicate. Data are presented as mean ± SEM of one experiment representative of at least two independent experiments.

    Journal: EBioMedicine

    Article Title: Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells

    doi: 10.1016/j.ebiom.2017.07.027

    Figure Lengend Snippet: Generation and characterization of mouse monolayer IECs from primary small intestinal organoids. (a) Mouse organoid-derived cells were broken by a 26G needle and seeded in type I collagen-coated Transwells. After they were cultured with mouse organoid culture medium supplemented with 300 ng/mL recombinant mWnt3a, TER values were measured on the indicated days in quadruplicate. Data are presented as mean ± SEM of one experiment representative of three independent experiments. (b) Immunohistochemistry analysis of the monolayer harvested with the Transwell membrane at 12 days of culture. Sections were stained with anti-E-cadherin (green) and anti-villin1 (red) antibodies, and counterstained with DAPI (blue). The right image is a magnification of the boxed region. Data are representative of three independent experiments. Scale bar, 20 μm. (c) Whole-mount images of the cells visualized by confocal laser microscopy after fixing and staining with antibodies toward the indicated molecules. Arrowheads indicate each positive cell. Data are representative of two independent experiments. Scale bars, 50 μm. (d–f) Mouse monolayer IECs that had been cultured for 13 days in Transwells and then replaced by pre-stimulation medium to starve cytokines for 1 day, followed by the stimulation with various concentrations of the indicated cytokines for 2 days. The relative mRNA level was determined by quantitative RT-PCR and normalized to GAPDH mRNA levels. The assay was performed in triplicate. Data are presented as mean ± SEM of one experiment representative of at least two independent experiments.

    Article Snippet: Reverse transcription was then performed using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems), and mRNA levels were quantified by fluorescence real-time PCR on a StepOnePlus (Applied Biosystems) using TaqMan Gene Expression Assays (Applied Biosystems) or PrimeTime qPCR Assays (Integrated DNA Technologies).

    Techniques: Derivative Assay, Cell Culture, Recombinant, Immunohistochemistry, Staining, Microscopy, Quantitative RT-PCR

    Inflammatory induction in monolayer IECs by co-culturing with differentiated adipocytes. (a) Images of differentiated mouse embryonic fibroblasts (MEFs; days 0, 9, and 30) stained with Oil Red O. (b) Schematic of co-culture system for IECs and adipocytes. (c) Monolayer IECs derived from mouse small intestinal organoids were cultured for 13 days in Transwells, and then co-cultured in pre-stimulation medium for 2 days with MEFs (days 0, 7, and 26). IECs were then harvested, and the relative mRNA levels were determined by quantitative RT-PCR and normalized to GAPDH mRNA levels. (d) Images of differentiated human primary visceral adipocytes (days 0 and 14) stained with Oil Red O. (e) Monolayer IECs derived from human iPSC-derived organoids were cultured for 13 days in Transwells, and then co-cultured in pre-stimulation medium for 2 days with human primary adipocytes (days 0 and 11). IECs were then harvested, and the relative mRNA levels were determined by quantitative RT-PCR and normalized to GAPDH mRNA levels. The assay was performed in triplicate. Data are presented as mean ± SEM, * P

    Journal: EBioMedicine

    Article Title: Reciprocal Inflammatory Signaling Between Intestinal Epithelial Cells and Adipocytes in the Absence of Immune Cells

    doi: 10.1016/j.ebiom.2017.07.027

    Figure Lengend Snippet: Inflammatory induction in monolayer IECs by co-culturing with differentiated adipocytes. (a) Images of differentiated mouse embryonic fibroblasts (MEFs; days 0, 9, and 30) stained with Oil Red O. (b) Schematic of co-culture system for IECs and adipocytes. (c) Monolayer IECs derived from mouse small intestinal organoids were cultured for 13 days in Transwells, and then co-cultured in pre-stimulation medium for 2 days with MEFs (days 0, 7, and 26). IECs were then harvested, and the relative mRNA levels were determined by quantitative RT-PCR and normalized to GAPDH mRNA levels. (d) Images of differentiated human primary visceral adipocytes (days 0 and 14) stained with Oil Red O. (e) Monolayer IECs derived from human iPSC-derived organoids were cultured for 13 days in Transwells, and then co-cultured in pre-stimulation medium for 2 days with human primary adipocytes (days 0 and 11). IECs were then harvested, and the relative mRNA levels were determined by quantitative RT-PCR and normalized to GAPDH mRNA levels. The assay was performed in triplicate. Data are presented as mean ± SEM, * P

    Article Snippet: Reverse transcription was then performed using High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems), and mRNA levels were quantified by fluorescence real-time PCR on a StepOnePlus (Applied Biosystems) using TaqMan Gene Expression Assays (Applied Biosystems) or PrimeTime qPCR Assays (Integrated DNA Technologies).

    Techniques: Staining, Co-Culture Assay, Derivative Assay, Cell Culture, Quantitative RT-PCR

    Effect of the removal of the histone N-terminal tails on the thermal stabilities of the nucleosomes. Each nucleosome, containing tlH2A, tlH2B, tlH3, or tlH4 (final concentration, 2.25 μM), was mixed with SYPRO Orange, and the sample temperature was increased by the StepOnePlus™ Real-Time PCR unit (Applied Biosystems). The temperature gradient was from 25 to 95 °C, in steps of 1 °C/min. Fluorescence signals of SYPRO Orange bound to the denatured histones were measured, and the thermal denaturing curves of the wt, tlH2A, tlH2B, tlH3, and tlH4 nucleosomes are shown in black, yellow, pink, blue, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: FEBS Open Bio

    Article Title: Contribution of histone N-terminal tails to the structure and stability of nucleosomes

    doi: 10.1016/j.fob.2013.08.007

    Figure Lengend Snippet: Effect of the removal of the histone N-terminal tails on the thermal stabilities of the nucleosomes. Each nucleosome, containing tlH2A, tlH2B, tlH3, or tlH4 (final concentration, 2.25 μM), was mixed with SYPRO Orange, and the sample temperature was increased by the StepOnePlus™ Real-Time PCR unit (Applied Biosystems). The temperature gradient was from 25 to 95 °C, in steps of 1 °C/min. Fluorescence signals of SYPRO Orange bound to the denatured histones were measured, and the thermal denaturing curves of the wt, tlH2A, tlH2B, tlH3, and tlH4 nucleosomes are shown in black, yellow, pink, blue, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: The sample temperature was increased by the StepOnePlus™ Real-Time PCR unit (Applied Biosystems), and the fluorescence signals were measured with this system.

    Techniques: Concentration Assay, Real-time Polymerase Chain Reaction, Fluorescence

    a Specificity of real-time PCR amplification melting temperature analysis of PCR products recorded in ABI 7500 Real-time PCR system (Applied Biosystems, Foster City, CA). Descriptions: NC - DNA extracted from non infected SPF chicken kidneys (CEKs). b Specificity NC - DNA extracted from non infected SPF chicken kidneys (CEKs). No real-time PCR curve was observed in the case of the negative control, nor for DNA obtained from other viruses: CAV, HEV, DAdV. Rel-time PCR curve was observed in the case of FAdV infection

    Journal: BMC Veterinary Research

    Article Title: Detection of fowl adenovirus D strains in wild birds in Poland by Loop-Mediated Isothermal Amplification (LAMP)

    doi: 10.1186/s12917-020-2271-4

    Figure Lengend Snippet: a Specificity of real-time PCR amplification melting temperature analysis of PCR products recorded in ABI 7500 Real-time PCR system (Applied Biosystems, Foster City, CA). Descriptions: NC - DNA extracted from non infected SPF chicken kidneys (CEKs). b Specificity NC - DNA extracted from non infected SPF chicken kidneys (CEKs). No real-time PCR curve was observed in the case of the negative control, nor for DNA obtained from other viruses: CAV, HEV, DAdV. Rel-time PCR curve was observed in the case of FAdV infection

    Article Snippet: The real-time PCR have been performed by using Applied Biosystems 7500 Real-time PCR, and were used in a final volume of 25 μl.

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction, Infection, Negative Control

    Expression of oppA mRNA transcripts in cultured B. burgdorferi . Expression of the oppA genes was measured by RT PCR (A) and by RPA (B). For RT PCR, RNA was isolated from B. burgdorferi grown in BSK H medium at 37°C. cDNA was generated from total RNA, using gene-specific primers. For each sample, quantitative RT PCR for oppA-I to -V was performed. Determination of copy numbers was calculated by comparison to individual standard curves generated for each primer set. Panel A shows the results of three separate experiments. For RPA, total RNA was hybridized to biotinylated probes specific for each oppA gene and then digested with RNase A-RNase T 1 . Samples were subjected to electrophoresis in a polyacrylamide gel and transferred to nylon membranes. Detection of biotinylated probes was done using chemiluminescence, and quantitation was performed by scanning densitometry. Data shown are individual results from three separate experiments.

    Journal: Journal of Bacteriology

    Article Title: Effects of Environmental Changes on Expression of the Oligopeptide Permease (opp) Genes of Borrelia burgdorferi

    doi: 10.1128/JB.184.22.6198-6206.2002

    Figure Lengend Snippet: Expression of oppA mRNA transcripts in cultured B. burgdorferi . Expression of the oppA genes was measured by RT PCR (A) and by RPA (B). For RT PCR, RNA was isolated from B. burgdorferi grown in BSK H medium at 37°C. cDNA was generated from total RNA, using gene-specific primers. For each sample, quantitative RT PCR for oppA-I to -V was performed. Determination of copy numbers was calculated by comparison to individual standard curves generated for each primer set. Panel A shows the results of three separate experiments. For RPA, total RNA was hybridized to biotinylated probes specific for each oppA gene and then digested with RNase A-RNase T 1 . Samples were subjected to electrophoresis in a polyacrylamide gel and transferred to nylon membranes. Detection of biotinylated probes was done using chemiluminescence, and quantitation was performed by scanning densitometry. Data shown are individual results from three separate experiments.

    Article Snippet: The generated cDNA was used as a template for real-time PCR amplification (ABI 7700; Applied Biosystems), using SYBR green fluorescent dye (SYBR Green Master Mix; Applied Biosystems) and specific primers for each oppA gene.

    Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Recombinase Polymerase Amplification, Isolation, Generated, Quantitative RT-PCR, Electrophoresis, Quantitation Assay

    mRNA and protein expression for AR in TG of male and female rats. (A) PCR of TG cDNA produced a single band of the expected size for AR (278 bp, A) in male and female TG. M, marker, NTS, no template control. (B) Bar graphs showing quantitative comparisons

    Journal: Neuroscience

    Article Title: THE ROLE OF ANDROGEN RECEPTOR IN TRANSCRIPTIONAL MODULATION OF CANNABINOID RECEPTOR TYPE 1 GENE IN RAT TRIGEMINAL GANGLIA

    doi: 10.1016/j.neuroscience.2013.09.014

    Figure Lengend Snippet: mRNA and protein expression for AR in TG of male and female rats. (A) PCR of TG cDNA produced a single band of the expected size for AR (278 bp, A) in male and female TG. M, marker, NTS, no template control. (B) Bar graphs showing quantitative comparisons

    Article Snippet: To assess cytokine-induced changes in CB1 mRNA level, and to compare AR and IL-1β receptor (IL-1R) expression between male and female TG, real-time PCR analysis of cDNA equal to 25 ng RNA was performed on the Eppendorf Mastercycler ep realplex 2.0.

    Techniques: Expressing, Polymerase Chain Reaction, Produced, Marker

    The amplification plot of FMDV isolates with classical rRT-PCR reagents and AuNPs-FMDV biosensor: the amplification plot is showing amplification of FMDV isolates with classical rRT-PCR reagents (yellow and violet) curves with CT values 7.03 and 15.9 and with AuNPs-FMDV biosensor (red and blue) curves with CT values 4.09 and 12.93

    Journal: Journal of Nanobiotechnology

    Article Title: Development of gold nanoparticles biosensor for ultrasensitive diagnosis of foot and mouth disease virus

    doi: 10.1186/s12951-018-0374-x

    Figure Lengend Snippet: The amplification plot of FMDV isolates with classical rRT-PCR reagents and AuNPs-FMDV biosensor: the amplification plot is showing amplification of FMDV isolates with classical rRT-PCR reagents (yellow and violet) curves with CT values 7.03 and 15.9 and with AuNPs-FMDV biosensor (red and blue) curves with CT values 4.09 and 12.93

    Article Snippet: FMDV rRT-PCR was done using QuantiTect Kit (Qiagen) in a real-time PCR machine (StepOne, Applied Biosystems) with a thermal profile according to the manufacturer’s instructions.

    Techniques: Amplification, Quantitative RT-PCR

    Effect of miR-31 on cisplatin sensitivity and invasion capacity of DDP-resistant cells A. The affirmation of the level of miR-31 mRNA expressed in GBC-SD/DDP and NOZ/DDP cells transfected with miR-31 mimic by qRT-PCR. B. The cell viability plotted against the concentration of DDP in GBC-SD/DPP and NOZ/DPP cells transfected with miR-31 or vector. C. Colony formation assay of DDP-resistant cells transfected with miR-31 or vector after exposure to DDP. D. The DDP-induced apoptosis rate of GBC-SD/DPP and NOZ/DPP cells transfected with miR-31 or vector analyzed by flow cytometry. E. The invasive ability of DDP-resistant cells transfected with miR-31 or vector after treatment with DDP in the transwell invasion assay. Data were presented as mean ± SD. * P

    Journal: Oncotarget

    Article Title: MiR-31 regulates the cisplatin resistance by targeting Src in gallbladder cancer

    doi: 10.18632/oncotarget.13067

    Figure Lengend Snippet: Effect of miR-31 on cisplatin sensitivity and invasion capacity of DDP-resistant cells A. The affirmation of the level of miR-31 mRNA expressed in GBC-SD/DDP and NOZ/DDP cells transfected with miR-31 mimic by qRT-PCR. B. The cell viability plotted against the concentration of DDP in GBC-SD/DPP and NOZ/DPP cells transfected with miR-31 or vector. C. Colony formation assay of DDP-resistant cells transfected with miR-31 or vector after exposure to DDP. D. The DDP-induced apoptosis rate of GBC-SD/DPP and NOZ/DPP cells transfected with miR-31 or vector analyzed by flow cytometry. E. The invasive ability of DDP-resistant cells transfected with miR-31 or vector after treatment with DDP in the transwell invasion assay. Data were presented as mean ± SD. * P

    Article Snippet: To measure the level of miR-31, quantitative real-time PCR (qRT-PCR) was performed on an ABI 7900HT fast real-time PCR system (Applied Biosystems, FosterCity, CA, USA) according to TaqMan Small RNA Assays protocol.

    Techniques: Transfection, Quantitative RT-PCR, Concentration Assay, Plasmid Preparation, Colony Assay, Flow Cytometry, Cytometry, Transwell Invasion Assay

    Src is a direct target gene of miR-31 and inversely correlated with miR-31 in GBC patients A. Sequence of the miR-31-binding site within the human Src 3′-UTR and a schematic diagram of the reporter construct showing the entire Src 3′-UTR sequence and the mutated Src 3′-UTR sequence. Src-WT: wild-type; Src-MUT: mutant type B. Luciferase assay of NOZ/DDP cells co-transfected with vector or miR-31 and a luciferase reporter containing the full length of Src 3′-UTR (WT) or a mutant (MUT). Luciferase activities were measured 24 hours post-transfection. Src-WT: wild-type; Src-MUT: mutant type C. The expression level of Src measured by qRT-PCR and normalized to GAPDH. D. The immunoblotting data of Src and p-Src(Y416) expression in DDP-resistant GBC cells transfected with miR-31 or vector. E. The mRNA expression of Src in tumor tissues and the adjacent non-tumor tissues from 41 GBC patients by qRT-PCR. F. An inverse correlation between miR-31 and Src mRNA expression by Pearson correlation analysis (Pearson's correlation: r= −0.56, P

    Journal: Oncotarget

    Article Title: MiR-31 regulates the cisplatin resistance by targeting Src in gallbladder cancer

    doi: 10.18632/oncotarget.13067

    Figure Lengend Snippet: Src is a direct target gene of miR-31 and inversely correlated with miR-31 in GBC patients A. Sequence of the miR-31-binding site within the human Src 3′-UTR and a schematic diagram of the reporter construct showing the entire Src 3′-UTR sequence and the mutated Src 3′-UTR sequence. Src-WT: wild-type; Src-MUT: mutant type B. Luciferase assay of NOZ/DDP cells co-transfected with vector or miR-31 and a luciferase reporter containing the full length of Src 3′-UTR (WT) or a mutant (MUT). Luciferase activities were measured 24 hours post-transfection. Src-WT: wild-type; Src-MUT: mutant type C. The expression level of Src measured by qRT-PCR and normalized to GAPDH. D. The immunoblotting data of Src and p-Src(Y416) expression in DDP-resistant GBC cells transfected with miR-31 or vector. E. The mRNA expression of Src in tumor tissues and the adjacent non-tumor tissues from 41 GBC patients by qRT-PCR. F. An inverse correlation between miR-31 and Src mRNA expression by Pearson correlation analysis (Pearson's correlation: r= −0.56, P

    Article Snippet: To measure the level of miR-31, quantitative real-time PCR (qRT-PCR) was performed on an ABI 7900HT fast real-time PCR system (Applied Biosystems, FosterCity, CA, USA) according to TaqMan Small RNA Assays protocol.

    Techniques: Sequencing, Binding Assay, Construct, Mutagenesis, Luciferase, Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR

    MiR-31 increases chemosensitivity of NOZ/DDP cells in vivo A. The xenograft tumors excised from the nude mice subcutaneously injected NOZ/DDP cells transfected with miR-31 or vector with or without treatment with DDP. B. The effect of miR-31 on the volume of xenograft tumors treated with DDP. C. The effect of miR-31 on the weight of xenograft tumors treated with DDP. D, E. The expression levels of miR-31and Src mRNA of xenograft tumors quantified by qRT-PCR. Data were presented as mean ± SD. * P

    Journal: Oncotarget

    Article Title: MiR-31 regulates the cisplatin resistance by targeting Src in gallbladder cancer

    doi: 10.18632/oncotarget.13067

    Figure Lengend Snippet: MiR-31 increases chemosensitivity of NOZ/DDP cells in vivo A. The xenograft tumors excised from the nude mice subcutaneously injected NOZ/DDP cells transfected with miR-31 or vector with or without treatment with DDP. B. The effect of miR-31 on the volume of xenograft tumors treated with DDP. C. The effect of miR-31 on the weight of xenograft tumors treated with DDP. D, E. The expression levels of miR-31and Src mRNA of xenograft tumors quantified by qRT-PCR. Data were presented as mean ± SD. * P

    Article Snippet: To measure the level of miR-31, quantitative real-time PCR (qRT-PCR) was performed on an ABI 7900HT fast real-time PCR system (Applied Biosystems, FosterCity, CA, USA) according to TaqMan Small RNA Assays protocol.

    Techniques: In Vivo, Mouse Assay, Injection, Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR

    Down-regulation of miR-31 in DDP-resistant GBC cells A. Heatmap representation of the expression difference of miRNAs in the GBC-SD, NOZ and the DDP-resistant GBC cells. The horizontal axis signifies the expression of miRNAs, and columns represent the biological replicates. Red, high expression; green, low expression. B. The expression level of miR-31 detected in DDP-resistant cells (GBC-SD/DDP and NOZ/DDP cells) and in its parental cells by qRT-PCR. Data were presented as mean ± SD. ** P

    Journal: Oncotarget

    Article Title: MiR-31 regulates the cisplatin resistance by targeting Src in gallbladder cancer

    doi: 10.18632/oncotarget.13067

    Figure Lengend Snippet: Down-regulation of miR-31 in DDP-resistant GBC cells A. Heatmap representation of the expression difference of miRNAs in the GBC-SD, NOZ and the DDP-resistant GBC cells. The horizontal axis signifies the expression of miRNAs, and columns represent the biological replicates. Red, high expression; green, low expression. B. The expression level of miR-31 detected in DDP-resistant cells (GBC-SD/DDP and NOZ/DDP cells) and in its parental cells by qRT-PCR. Data were presented as mean ± SD. ** P

    Article Snippet: To measure the level of miR-31, quantitative real-time PCR (qRT-PCR) was performed on an ABI 7900HT fast real-time PCR system (Applied Biosystems, FosterCity, CA, USA) according to TaqMan Small RNA Assays protocol.

    Techniques: Expressing, Quantitative RT-PCR

    The tissue RARRES2 gene expression level is down-regulated in ACC but is not associated with patient prognosis. A, TaqMan real-time quantitative PCR reveals down-regulation of the RARRES2 gene expression level in our cohort of ACC samples as compared

    Journal: The Journal of Clinical Endocrinology and Metabolism

    Article Title: Serum RARRES2 Is a Prognostic Marker in Patients With Adrenocortical Carcinoma

    doi: 10.1210/jc.2016-1781

    Figure Lengend Snippet: The tissue RARRES2 gene expression level is down-regulated in ACC but is not associated with patient prognosis. A, TaqMan real-time quantitative PCR reveals down-regulation of the RARRES2 gene expression level in our cohort of ACC samples as compared

    Article Snippet: TaqMan real-time quantitative polymerase chain reaction was performed on 7900HT fast real-time PCR systems (Applied Biosystems).

    Techniques: Expressing, Real-time Polymerase Chain Reaction