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  • 99
    Thermo Fisher realtime pcr
    STING mutants fail to respond to DNA-adduct forming agents. The reconstituted primary Sting −/− MEF cells with empty vector (pBabe), hSTING, R169W, or P203S were treated DMBA (20 μg/ml) or cisplatin (10 μM) for 48 hr. Total RNA was extracted and <t>realtime</t> <t>PCR</t> was performed with the indicated probes after cDNA synthesis. Data shown here are the averages ± SD (n = 3). Asterisks indicate significant difference (p
    Realtime Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1416 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/realtime pcr/product/Thermo Fisher
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    99
    Bruker Corporation real time pcr system
    STING mutants fail to respond to DNA-adduct forming agents. The reconstituted primary Sting −/− MEF cells with empty vector (pBabe), hSTING, R169W, or P203S were treated DMBA (20 μg/ml) or cisplatin (10 μM) for 48 hr. Total RNA was extracted and <t>realtime</t> <t>PCR</t> was performed with the indicated probes after cDNA synthesis. Data shown here are the averages ± SD (n = 3). Asterisks indicate significant difference (p
    Real Time Pcr System, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr system/product/Bruker Corporation
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    real time pcr system - by Bioz Stars, 2020-08
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    Thermo Fisher realtime pcr system
    GLI1 participates in the process of the embryonic neurogenesis. ( a ) Sketch map shows the different region selected for ChIP- <t>realtime-PCR.</t> ( b ) Flag-H3.3 binds to the Gli1 promoter region. However, the binding of Flag-H3.3K36R to each region was decreased versus the Flag-H3.3 in the NSCs ( n =3 independent experiments; bars represent mean±S.E.M). ( c ) Flag-H4 binds to the Gli1 promoter and the gene body. However, the binding of Flag-H4K16R and Flag-H4K16A to each region was decreased versus the Flag-H4 in the NSCs ( n =3 independent experiments; bars represent mean±S.E.M). ( d ) H4K16ac binds to the region of the Gli1 promoter in the NSCs ( n =3 independent experiments; bars represent mean±S.E.M). ( e , f ) GLI1 knockdown causes Gli1 -shRNA-GFP cell positioning changes in utero. The empty shRNA control or GLI1 knockdown plasmids was electroporated into E13.5 embryonic mouse brains, and the phenotype was analyzed at E17.5. The percentage of Gli1 -shRNA-GFP or Control-GFP cells in each region is shown ( n =3 independent experiments; error bars represent mean±SEM; * P
    Realtime Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/realtime pcr system/product/Thermo Fisher
    Average 99 stars, based on 285 article reviews
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    Thermo Fisher real time pcr stepone real time pcr system
    Effect of esculetin on apoptosis- and cell cycle-related gene expression. The cells were treated with 25 μM, 75 μM, 150 μM, and 300 μM of esculetin for 48 hours, and then targeted <t>mRNA</t> expressions were analyzed by reverse transcription and quantitative real-time <t>PCR.</t> (A) The fold changes of apoptosis-associated genes and (B) cell cycle related genes were plotted on graphs. For each group, two different experiments were performed in triplicate (mean ± SD). * P
    Real Time Pcr Stepone Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher steponeplus realtime pcr system
    Effect of esculetin on apoptosis- and cell cycle-related gene expression. The cells were treated with 25 μM, 75 μM, 150 μM, and 300 μM of esculetin for 48 hours, and then targeted <t>mRNA</t> expressions were analyzed by reverse transcription and quantitative real-time <t>PCR.</t> (A) The fold changes of apoptosis-associated genes and (B) cell cycle related genes were plotted on graphs. For each group, two different experiments were performed in triplicate (mean ± SD). * P
    Steponeplus Realtime Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time pcr real time pcr
    SIRT1 expression levels decrease after <t>miR-34a</t> overexpression. miR-34a expression was analyzed by quantitative Real <t>Time-PCR</t> using specific Taqman primers and GAPDH for normalization. Expression levels were calculated by the ΔΔCt method using undifferentiated cells as calibrator. SIRT1 expression in mouse NS cells was detected by immunoblotting at different times of differentiation or by immunocytochemistry at 2 days of differentiation. Cells transfected with either control or pre-miR-34a at 3 days of differentiation were also processed for SIRT1 detection by immunoblotting. A. Expression of miR-34a throughout the differentiation period. * p
    Real Time Pcr Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 912 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr real time pcr/product/Thermo Fisher
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    96
    Thermo Fisher quantitative real time pcr
    KLF15 is the major Kruppel-Like Factor in HPMEC and important regulator of critical endothelial enzymes- Arginase 2 and eNOS Total RNA from Human Pulmonary Microvascular endothelial cells was converted to <t>cDNA</t> and the relative mRNA expression of KLF15 was determined in quiescent HPMEC using real-time <t>PCR</t> (N=3), B) Cell lysates from HPMEC, human coronary artery endothelial cells (HCAEC), Human Aortic Endothelial Cells (HAEC), Human Aortic Smooth Muscle Cells (HASMC) were subjected to western blotting with KLF15, eNOS and β-tubulin antibodies (N=4). Cell lysates from HPMEC overexpressing HA-KLF15 was used as a positive control, C) Total RNA from Human Pulmonary Microvascular endothelial cells was converted to cDNA and the relative mRNA expression of KLF2, 4 and 15 was determined in quiescent HPMEC using real-time PCR (N=3) *p
    Quantitative Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 45481 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    STING mutants fail to respond to DNA-adduct forming agents. The reconstituted primary Sting −/− MEF cells with empty vector (pBabe), hSTING, R169W, or P203S were treated DMBA (20 μg/ml) or cisplatin (10 μM) for 48 hr. Total RNA was extracted and realtime PCR was performed with the indicated probes after cDNA synthesis. Data shown here are the averages ± SD (n = 3). Asterisks indicate significant difference (p

    Journal: Oncogene

    Article Title: Suppression of STING Signaling through Epigenetic Silencing and Missense Mutation Impedes DNA-Damage Mediated Cytokine Production

    doi: 10.1038/s41388-017-0120-0

    Figure Lengend Snippet: STING mutants fail to respond to DNA-adduct forming agents. The reconstituted primary Sting −/− MEF cells with empty vector (pBabe), hSTING, R169W, or P203S were treated DMBA (20 μg/ml) or cisplatin (10 μM) for 48 hr. Total RNA was extracted and realtime PCR was performed with the indicated probes after cDNA synthesis. Data shown here are the averages ± SD (n = 3). Asterisks indicate significant difference (p

    Article Snippet: Realtime PCR was performed using StepOnePlus Real-Time PCR system (Applied Biosystems).

    Techniques: Plasmid Preparation, Polymerase Chain Reaction

    GLI1 participates in the process of the embryonic neurogenesis. ( a ) Sketch map shows the different region selected for ChIP- realtime-PCR. ( b ) Flag-H3.3 binds to the Gli1 promoter region. However, the binding of Flag-H3.3K36R to each region was decreased versus the Flag-H3.3 in the NSCs ( n =3 independent experiments; bars represent mean±S.E.M). ( c ) Flag-H4 binds to the Gli1 promoter and the gene body. However, the binding of Flag-H4K16R and Flag-H4K16A to each region was decreased versus the Flag-H4 in the NSCs ( n =3 independent experiments; bars represent mean±S.E.M). ( d ) H4K16ac binds to the region of the Gli1 promoter in the NSCs ( n =3 independent experiments; bars represent mean±S.E.M). ( e , f ) GLI1 knockdown causes Gli1 -shRNA-GFP cell positioning changes in utero. The empty shRNA control or GLI1 knockdown plasmids was electroporated into E13.5 embryonic mouse brains, and the phenotype was analyzed at E17.5. The percentage of Gli1 -shRNA-GFP or Control-GFP cells in each region is shown ( n =3 independent experiments; error bars represent mean±SEM; * P

    Journal: Cell Death and Differentiation

    Article Title: Histone variant H3.3 orchestrates neural stem cell differentiation in the developing brain

    doi: 10.1038/cdd.2017.77

    Figure Lengend Snippet: GLI1 participates in the process of the embryonic neurogenesis. ( a ) Sketch map shows the different region selected for ChIP- realtime-PCR. ( b ) Flag-H3.3 binds to the Gli1 promoter region. However, the binding of Flag-H3.3K36R to each region was decreased versus the Flag-H3.3 in the NSCs ( n =3 independent experiments; bars represent mean±S.E.M). ( c ) Flag-H4 binds to the Gli1 promoter and the gene body. However, the binding of Flag-H4K16R and Flag-H4K16A to each region was decreased versus the Flag-H4 in the NSCs ( n =3 independent experiments; bars represent mean±S.E.M). ( d ) H4K16ac binds to the region of the Gli1 promoter in the NSCs ( n =3 independent experiments; bars represent mean±S.E.M). ( e , f ) GLI1 knockdown causes Gli1 -shRNA-GFP cell positioning changes in utero. The empty shRNA control or GLI1 knockdown plasmids was electroporated into E13.5 embryonic mouse brains, and the phenotype was analyzed at E17.5. The percentage of Gli1 -shRNA-GFP or Control-GFP cells in each region is shown ( n =3 independent experiments; error bars represent mean±SEM; * P

    Article Snippet: Realtime-PCR was performed on the realtime-PCR machine (ABI 7500, Life Technologies, Singapore).

    Techniques: Chromatin Immunoprecipitation, Polymerase Chain Reaction, Binding Assay, shRNA, In Utero

    GLI zinc finger family is highly suppressed in H3.3 knockdown NSCs. ( a , b ) The volcano map ( a ) and the heat map ( b ) show that the gene profiling expression. Genes analysis reveals that transcript levels of 1081 genes were upregulated or downregulated by more than two-fold between the H3.3 knockdown and the control. ( c , d ) Enrichment analysis for biological process. Gene ontology (GO) terms associated with the genes downregulated (c) or upregulated ( d ) in H3.3 knockdown NSCs versus the control. ( e ) RNA-seq analysis shows the mRNAs expression level of GLI family members are dramatic reduced in H3.3 knockdown NSCs versus the control. ( f ) Realtime-PCR analysis of GLI family members gene expression in H3.3 knockdown NSCs versus the control ( n =3 independent experiments; bar represents mean±S.E.M; ** P

    Journal: Cell Death and Differentiation

    Article Title: Histone variant H3.3 orchestrates neural stem cell differentiation in the developing brain

    doi: 10.1038/cdd.2017.77

    Figure Lengend Snippet: GLI zinc finger family is highly suppressed in H3.3 knockdown NSCs. ( a , b ) The volcano map ( a ) and the heat map ( b ) show that the gene profiling expression. Genes analysis reveals that transcript levels of 1081 genes were upregulated or downregulated by more than two-fold between the H3.3 knockdown and the control. ( c , d ) Enrichment analysis for biological process. Gene ontology (GO) terms associated with the genes downregulated (c) or upregulated ( d ) in H3.3 knockdown NSCs versus the control. ( e ) RNA-seq analysis shows the mRNAs expression level of GLI family members are dramatic reduced in H3.3 knockdown NSCs versus the control. ( f ) Realtime-PCR analysis of GLI family members gene expression in H3.3 knockdown NSCs versus the control ( n =3 independent experiments; bar represents mean±S.E.M; ** P

    Article Snippet: Realtime-PCR was performed on the realtime-PCR machine (ABI 7500, Life Technologies, Singapore).

    Techniques: Expressing, RNA Sequencing Assay, Polymerase Chain Reaction

    Transient expression of AtHPT, AtTC and AtTC + AtHPT post agroinfiltration by reverse transcription PCR. Top panel: Target genes Lane 1 Wild type control Lane 2 HPT Lane 3 TC Lane 3 HPT + TC with TC primers Lane 3 HPT + TC with HPT primers Lane 6 HPT + TC with both HPT TC primers. Bottom panel: Reference gene F-box

    Journal: 3 Biotech

    Article Title: Rapid enhancement of α-tocopherol content in Nicotiana benthamiana by transient expression of Arabidopsis thaliana Tocopherol cyclase and Homogentisate phytyl transferase genes

    doi: 10.1007/s13205-018-1496-4

    Figure Lengend Snippet: Transient expression of AtHPT, AtTC and AtTC + AtHPT post agroinfiltration by reverse transcription PCR. Top panel: Target genes Lane 1 Wild type control Lane 2 HPT Lane 3 TC Lane 3 HPT + TC with TC primers Lane 3 HPT + TC with HPT primers Lane 6 HPT + TC with both HPT TC primers. Bottom panel: Reference gene F-box

    Article Snippet: Quantitive gene expression of both HPT and TC was analysed using Real-Time PCR system (Applied Biosystems, USA).

    Techniques: Expressing, Polymerase Chain Reaction

    Technical validation for microarray results using realtime PCR: Correlation of fold changes between microarray and realtime PCR.

    Journal: BMC Genomics

    Article Title: Use of a bovine genome array to identify new biological pathways for beef marbling in Hanwoo (Korean Cattle)

    doi: 10.1186/1471-2164-11-623

    Figure Lengend Snippet: Technical validation for microarray results using realtime PCR: Correlation of fold changes between microarray and realtime PCR.

    Article Snippet: The PCR was conducted in ABI 7500 realtime PCR system (Applied Biosystems, USA).

    Techniques: Microarray, Polymerase Chain Reaction

    Effect of esculetin on apoptosis- and cell cycle-related gene expression. The cells were treated with 25 μM, 75 μM, 150 μM, and 300 μM of esculetin for 48 hours, and then targeted mRNA expressions were analyzed by reverse transcription and quantitative real-time PCR. (A) The fold changes of apoptosis-associated genes and (B) cell cycle related genes were plotted on graphs. For each group, two different experiments were performed in triplicate (mean ± SD). * P

    Journal: Journal of Cancer Prevention

    Article Title: Esculetin Inhibits the Survival of Human Prostate Cancer Cells by Inducing Apoptosis and Arresting the Cell Cycle

    doi: 10.15430/JCP.2018.23.1.10

    Figure Lengend Snippet: Effect of esculetin on apoptosis- and cell cycle-related gene expression. The cells were treated with 25 μM, 75 μM, 150 μM, and 300 μM of esculetin for 48 hours, and then targeted mRNA expressions were analyzed by reverse transcription and quantitative real-time PCR. (A) The fold changes of apoptosis-associated genes and (B) cell cycle related genes were plotted on graphs. For each group, two different experiments were performed in triplicate (mean ± SD). * P

    Article Snippet: Quantitative mRNA expression analyses were achieved on a Real-Time PCR Systems (Applied Biosystems, Foster City, CA, USA) using SYBR Green PCR Master Mix (Thermo Fisher Scientific).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Correlations between cytokine mRNA expression (derived from Realtime-PCR) and ruminal factors. a - c : Correlation analysis between IL-1 , IL-2 , IL-6 relative mRNA expressions and ruminal pH. d - f : Correlation analysis between IL-1 , IL-2 , IL-6 relative mRNA expressions and ruminal LPS

    Journal: Journal of Animal Science and Biotechnology

    Article Title: High-concentrate feeding upregulates the expression of inflammation-related genes in the ruminal epithelium of dairy cattle

    doi: 10.1186/s40104-016-0100-1

    Figure Lengend Snippet: Correlations between cytokine mRNA expression (derived from Realtime-PCR) and ruminal factors. a - c : Correlation analysis between IL-1 , IL-2 , IL-6 relative mRNA expressions and ruminal pH. d - f : Correlation analysis between IL-1 , IL-2 , IL-6 relative mRNA expressions and ruminal LPS

    Article Snippet: Reactions were run in a StepOne Plus Realtime PCR System (Applied Biosystems).

    Techniques: Expressing, Derivative Assay, Polymerase Chain Reaction

    SIRT1 expression levels decrease after miR-34a overexpression. miR-34a expression was analyzed by quantitative Real Time-PCR using specific Taqman primers and GAPDH for normalization. Expression levels were calculated by the ΔΔCt method using undifferentiated cells as calibrator. SIRT1 expression in mouse NS cells was detected by immunoblotting at different times of differentiation or by immunocytochemistry at 2 days of differentiation. Cells transfected with either control or pre-miR-34a at 3 days of differentiation were also processed for SIRT1 detection by immunoblotting. A. Expression of miR-34a throughout the differentiation period. * p

    Journal: PLoS ONE

    Article Title: miR-34a Regulates Mouse Neural Stem Cell Differentiation

    doi: 10.1371/journal.pone.0021396

    Figure Lengend Snippet: SIRT1 expression levels decrease after miR-34a overexpression. miR-34a expression was analyzed by quantitative Real Time-PCR using specific Taqman primers and GAPDH for normalization. Expression levels were calculated by the ΔΔCt method using undifferentiated cells as calibrator. SIRT1 expression in mouse NS cells was detected by immunoblotting at different times of differentiation or by immunocytochemistry at 2 days of differentiation. Cells transfected with either control or pre-miR-34a at 3 days of differentiation were also processed for SIRT1 detection by immunoblotting. A. Expression of miR-34a throughout the differentiation period. * p

    Article Snippet: Evaluation of miR-34a expression levels by quantitative Real Time-PCR Real-time PCR was performed in an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Techniques: Expressing, Over Expression, Real-time Polymerase Chain Reaction, Immunocytochemistry, Transfection

    KLF15 is the major Kruppel-Like Factor in HPMEC and important regulator of critical endothelial enzymes- Arginase 2 and eNOS Total RNA from Human Pulmonary Microvascular endothelial cells was converted to cDNA and the relative mRNA expression of KLF15 was determined in quiescent HPMEC using real-time PCR (N=3), B) Cell lysates from HPMEC, human coronary artery endothelial cells (HCAEC), Human Aortic Endothelial Cells (HAEC), Human Aortic Smooth Muscle Cells (HASMC) were subjected to western blotting with KLF15, eNOS and β-tubulin antibodies (N=4). Cell lysates from HPMEC overexpressing HA-KLF15 was used as a positive control, C) Total RNA from Human Pulmonary Microvascular endothelial cells was converted to cDNA and the relative mRNA expression of KLF2, 4 and 15 was determined in quiescent HPMEC using real-time PCR (N=3) *p

    Journal: Arteriosclerosis, thrombosis, and vascular biology

    Article Title: Hypoxia Triggers SENP1 Modulation of Kruppel-Like Factor 15 and Transcriptional Regulation of Arginase 2 in Pulmonary Endothelium

    doi: 10.1161/ATVBAHA.117.310660

    Figure Lengend Snippet: KLF15 is the major Kruppel-Like Factor in HPMEC and important regulator of critical endothelial enzymes- Arginase 2 and eNOS Total RNA from Human Pulmonary Microvascular endothelial cells was converted to cDNA and the relative mRNA expression of KLF15 was determined in quiescent HPMEC using real-time PCR (N=3), B) Cell lysates from HPMEC, human coronary artery endothelial cells (HCAEC), Human Aortic Endothelial Cells (HAEC), Human Aortic Smooth Muscle Cells (HASMC) were subjected to western blotting with KLF15, eNOS and β-tubulin antibodies (N=4). Cell lysates from HPMEC overexpressing HA-KLF15 was used as a positive control, C) Total RNA from Human Pulmonary Microvascular endothelial cells was converted to cDNA and the relative mRNA expression of KLF2, 4 and 15 was determined in quiescent HPMEC using real-time PCR (N=3) *p

    Article Snippet: RNA was then reverse transcribed with oligo dT primers to obtain cDNA and quantitative real time PCR (Applied Biosystems) was performed using SYBR Green Supermix mix (Applied Biosystems) whereas semi-quantitative RTPCR was performed using a conventional Biorad PCR machine and the following primer sets: Human Arg2 : Forward: 5′-GGG CCC TGA AGG CTG TAG-3′, Reverse: 5′-AAT GGA GCC ACT GCC ATC-3′.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Positive Control