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  • 97
    Thermo Fisher quantstudio real time pcr instrument
    Quantstudio Real Time Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantstudio real time pcr instrument/product/Thermo Fisher
    Average 97 stars, based on 41 article reviews
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    quantstudio real time pcr instrument - by Bioz Stars, 2020-07
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    99
    Thermo Fisher realtime pcr instrument
    Realtime Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/realtime pcr instrument/product/Thermo Fisher
    Average 99 stars, based on 98 article reviews
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    realtime pcr instrument - by Bioz Stars, 2020-07
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    91
    Thermo Fisher real time pcr instruments
    <t>miR-21</t> is overexpressed in high-grade intraepithelial neoplasia (HG-IEN) and Barrett's adenocarcinoma (BAc). (A) Predicted base complementarity of miR-21 to 3′-UTR binding site of PDCD4 , as predicted by the Sanger miR-database (miRBase, http://www.mirbase.org/ ). (B) Altered miR-21 expression between cancerous tissue (CT; HG-IEN and BAc) and non-cancerous tissue (NCT) in BAc patients by <t>qRT-PCR</t> analysis. miR-21 was significantly upregulated in HG-IEN and BAc samples (p
    Real Time Pcr Instruments, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr instruments/product/Thermo Fisher
    Average 91 stars, based on 265 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher stepone realtime pcr instrument
    <t>miR-21</t> is overexpressed in high-grade intraepithelial neoplasia (HG-IEN) and Barrett's adenocarcinoma (BAc). (A) Predicted base complementarity of miR-21 to 3′-UTR binding site of PDCD4 , as predicted by the Sanger miR-database (miRBase, http://www.mirbase.org/ ). (B) Altered miR-21 expression between cancerous tissue (CT; HG-IEN and BAc) and non-cancerous tissue (NCT) in BAc patients by <t>qRT-PCR</t> analysis. miR-21 was significantly upregulated in HG-IEN and BAc samples (p
    Stepone Realtime Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stepone realtime pcr instrument/product/Thermo Fisher
    Average 99 stars, based on 142 article reviews
    Price from $9.99 to $1999.99
    stepone realtime pcr instrument - by Bioz Stars, 2020-07
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    90
    Thermo Fisher real time pcr instrument
    Tumor stromal Cav-1 expression. Cav-1 expression was analysed using micro dissection with <t>qRT-PCR</t> analysis. This was performed on non-malignant prostate stroma (N), stroma from a Gleason grade 3 tumor area (G3) and stroma from a Gleason Grade 4 area (G4) (all from the same patient). The tissue was derived from four different Gleason score 7 (3+4) tumors. Cav-1 expression was highest in normal prostate tissue and decreased with cancer progression.
    Real Time Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher 7500 real time pcr instrument
    Tumor stromal Cav-1 expression. Cav-1 expression was analysed using micro dissection with <t>qRT-PCR</t> analysis. This was performed on non-malignant prostate stroma (N), stroma from a Gleason grade 3 tumor area (G3) and stroma from a Gleason Grade 4 area (G4) (all from the same patient). The tissue was derived from four different Gleason score 7 (3+4) tumors. Cav-1 expression was highest in normal prostate tissue and decreased with cancer progression.
    7500 Real Time Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 974 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher 7500 real time pcr instruments
    CTGF/CCN2 expression is regulated by dexamethasone in HC11 cells. HC11 mammary epithelial cells were grown to confluence and stimulated with dexamethasone (DEX)(1 μM) in serum-containing media in the presence of insulin. RU486 at varying concentrations or vehicle (ethanol) was added to cells alone or in combination with dexamethasone. RNA was isolated and levels of CTGF and actin RNA were determined by <t>realtime</t> <t>PCR.</t> The results indicate the induction of CTGF/CCN2 normalized to actin. * These Dex+Ru486 values represent statistically significant difference (p-value .001) from the Dex alone condition
    7500 Real Time Pcr Instruments, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 229 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Roche real time pcr instrument
    Necdin deficiency enhances adipocyte differentiation in adipose SV cells. ( A , B ) PPARγ + cells. SV cells prepared from Ndn +/+ and Ndn +m/−p littermates were treated with adipogenic inducers and double-stained for PPARγ (green) and nuclear <t>DNA</t> (blue)( A ). PPARγ + cells (arrowheads in A ) were counted (mean ± SEM, n = 3)( B ). ( C, D ) Expression levels of PPARγ2 and C/EBPα mRNAs. PPARγ2 ( C ) and C/EBPα ( D ) mRNA levels were analyzed by <t>qRT-PCR</t> 72 hr after adipogenic induction. ( E, F ) Oil Red O-staining. SV cells were stained with Oil Red O 8 days after adipogenic induction ( E ), and intracellular Oil Red O was quantified by spectrophotometry (mean ± SEM, n = 4–6)( F ). ( G, H ) Expression levels of aP2 and adiponectin mRNAs. The aP2 ( G ) and adiponectin ( H ) mRNA levels were analyzed by qRT-PCR 8 days after adipogenic induction (mean ± SEM, n = 3). All mRNA levels ( C, D, G, H ) are shown as relative values to GAPDH mRNA levels (mean ± SEM, n = 3) * P
    Real Time Pcr Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 1175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bioneer Corporation real time pcr instrument
    Overexpression of MBNL 1‐ AS 1 inhibited the proliferation and induced the apoptosis of BC cells. A, Relative expression of MBNL 1‐ AS 1 in 5637 and T24 cells was assessed by <t>qRT</t> ‐ <t>PCR</t> . B, The viable cells were evaluated using MTT assay in 5637 and T24 cells. C, Flow cytometry analysis was performed to determine the cell cycle distribution of 5637 and T24 cells. D, The percentage of apoptotic cells was detected by flow cytomentry in 5637 and T24 cells. * P
    Real Time Pcr Instrument, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    miR-21 is overexpressed in high-grade intraepithelial neoplasia (HG-IEN) and Barrett's adenocarcinoma (BAc). (A) Predicted base complementarity of miR-21 to 3′-UTR binding site of PDCD4 , as predicted by the Sanger miR-database (miRBase, http://www.mirbase.org/ ). (B) Altered miR-21 expression between cancerous tissue (CT; HG-IEN and BAc) and non-cancerous tissue (NCT) in BAc patients by qRT-PCR analysis. miR-21 was significantly upregulated in HG-IEN and BAc samples (p

    Journal: Journal of Clinical Pathology

    Article Title: Programmed cell death 4 (PDCD4) expression during multistep Barrett's carcinogenesis

    doi: 10.1136/jcp.2010.078253

    Figure Lengend Snippet: miR-21 is overexpressed in high-grade intraepithelial neoplasia (HG-IEN) and Barrett's adenocarcinoma (BAc). (A) Predicted base complementarity of miR-21 to 3′-UTR binding site of PDCD4 , as predicted by the Sanger miR-database (miRBase, http://www.mirbase.org/ ). (B) Altered miR-21 expression between cancerous tissue (CT; HG-IEN and BAc) and non-cancerous tissue (NCT) in BAc patients by qRT-PCR analysis. miR-21 was significantly upregulated in HG-IEN and BAc samples (p

    Article Snippet: The NCodeTM miRNA qRT-PCR method (Invitrogen, Carlsbad, California, USA) was used to detect and quantify mature miR-21 (primer sequence: 5′-CGGTAGCTTATCAGACTGATGTTGA-3′) on real-time PCR instruments according to the manufacturer's instructions (Applied Biosystems, Foster City, California, USA).

    Techniques: BAC Assay, Binding Assay, Expressing, Quantitative RT-PCR

    Tumor stromal Cav-1 expression. Cav-1 expression was analysed using micro dissection with qRT-PCR analysis. This was performed on non-malignant prostate stroma (N), stroma from a Gleason grade 3 tumor area (G3) and stroma from a Gleason Grade 4 area (G4) (all from the same patient). The tissue was derived from four different Gleason score 7 (3+4) tumors. Cav-1 expression was highest in normal prostate tissue and decreased with cancer progression.

    Journal: PLoS ONE

    Article Title: High Caveolin-1 Expression in Tumor Stroma Is Associated with a Favourable Outcome in Prostate Cancer Patients Managed by Watchful Waiting

    doi: 10.1371/journal.pone.0164016

    Figure Lengend Snippet: Tumor stromal Cav-1 expression. Cav-1 expression was analysed using micro dissection with qRT-PCR analysis. This was performed on non-malignant prostate stroma (N), stroma from a Gleason grade 3 tumor area (G3) and stroma from a Gleason Grade 4 area (G4) (all from the same patient). The tissue was derived from four different Gleason score 7 (3+4) tumors. Cav-1 expression was highest in normal prostate tissue and decreased with cancer progression.

    Article Snippet: The total RNA isolated from these patients was converted to cDNA using SuperScript III reverse transcriptase (Life Technologies, Carlsbad, CA, USA) and Cav-1 expression was analysed with qRT-PCR with an ABI 7900 HT fast Real-Time PCR instrument (Applied Biosystems, Foster City, CA, USA) using power SYBR green (Life Technologies).

    Techniques: Expressing, Dissection, Quantitative RT-PCR, Derivative Assay

    CTGF/CCN2 expression is regulated by dexamethasone in HC11 cells. HC11 mammary epithelial cells were grown to confluence and stimulated with dexamethasone (DEX)(1 μM) in serum-containing media in the presence of insulin. RU486 at varying concentrations or vehicle (ethanol) was added to cells alone or in combination with dexamethasone. RNA was isolated and levels of CTGF and actin RNA were determined by realtime PCR. The results indicate the induction of CTGF/CCN2 normalized to actin. * These Dex+Ru486 values represent statistically significant difference (p-value .001) from the Dex alone condition

    Journal: Journal of Cell Communication and Signaling

    Article Title: Global expression profiling reveals regulation of CTGF/CCN2 during lactogenic differentiation

    doi: 10.1007/s12079-009-0047-5

    Figure Lengend Snippet: CTGF/CCN2 expression is regulated by dexamethasone in HC11 cells. HC11 mammary epithelial cells were grown to confluence and stimulated with dexamethasone (DEX)(1 μM) in serum-containing media in the presence of insulin. RU486 at varying concentrations or vehicle (ethanol) was added to cells alone or in combination with dexamethasone. RNA was isolated and levels of CTGF and actin RNA were determined by realtime PCR. The results indicate the induction of CTGF/CCN2 normalized to actin. * These Dex+Ru486 values represent statistically significant difference (p-value .001) from the Dex alone condition

    Article Snippet: Realtime PCR was performed using SYBR green PCR kits and a 7500 RealTime PCR instrument (Applied Biosystems).

    Techniques: Expressing, Isolation, Polymerase Chain Reaction, Significance Assay

    Necdin deficiency enhances adipocyte differentiation in adipose SV cells. ( A , B ) PPARγ + cells. SV cells prepared from Ndn +/+ and Ndn +m/−p littermates were treated with adipogenic inducers and double-stained for PPARγ (green) and nuclear DNA (blue)( A ). PPARγ + cells (arrowheads in A ) were counted (mean ± SEM, n = 3)( B ). ( C, D ) Expression levels of PPARγ2 and C/EBPα mRNAs. PPARγ2 ( C ) and C/EBPα ( D ) mRNA levels were analyzed by qRT-PCR 72 hr after adipogenic induction. ( E, F ) Oil Red O-staining. SV cells were stained with Oil Red O 8 days after adipogenic induction ( E ), and intracellular Oil Red O was quantified by spectrophotometry (mean ± SEM, n = 4–6)( F ). ( G, H ) Expression levels of aP2 and adiponectin mRNAs. The aP2 ( G ) and adiponectin ( H ) mRNA levels were analyzed by qRT-PCR 8 days after adipogenic induction (mean ± SEM, n = 3). All mRNA levels ( C, D, G, H ) are shown as relative values to GAPDH mRNA levels (mean ± SEM, n = 3) * P

    Journal: PLoS ONE

    Article Title: Necdin Controls Proliferation of White Adipocyte Progenitor Cells

    doi: 10.1371/journal.pone.0030948

    Figure Lengend Snippet: Necdin deficiency enhances adipocyte differentiation in adipose SV cells. ( A , B ) PPARγ + cells. SV cells prepared from Ndn +/+ and Ndn +m/−p littermates were treated with adipogenic inducers and double-stained for PPARγ (green) and nuclear DNA (blue)( A ). PPARγ + cells (arrowheads in A ) were counted (mean ± SEM, n = 3)( B ). ( C, D ) Expression levels of PPARγ2 and C/EBPα mRNAs. PPARγ2 ( C ) and C/EBPα ( D ) mRNA levels were analyzed by qRT-PCR 72 hr after adipogenic induction. ( E, F ) Oil Red O-staining. SV cells were stained with Oil Red O 8 days after adipogenic induction ( E ), and intracellular Oil Red O was quantified by spectrophotometry (mean ± SEM, n = 4–6)( F ). ( G, H ) Expression levels of aP2 and adiponectin mRNAs. The aP2 ( G ) and adiponectin ( H ) mRNA levels were analyzed by qRT-PCR 8 days after adipogenic induction (mean ± SEM, n = 3). All mRNA levels ( C, D, G, H ) are shown as relative values to GAPDH mRNA levels (mean ± SEM, n = 3) * P

    Article Snippet: RT-PCR products were quantified using a real-time PCR instrument (LightCycler, Roche) and FastStart DNA MasterPLUS SYBR green I kit (Roche).

    Techniques: Staining, Expressing, Quantitative RT-PCR, Spectrophotometry

    Necdin is expressed in primary adipose SV cells. ( A ) Western blot analysis of endogenous necdin. WAT stromal-vascular fraction (SVF), adipocyte fraction (AF), pooled WAT (WAT), interscapular BAT (BAT), and brain were prepared from 5-week-old mice. Necdin in the extracts was analyzed by Western blotting. Molecular sizes are in kilodaltons (kDa). ( B ) qRT-PCR for necdin mRNA. Total RNA was extracted from the above fractions and tissues. Necdin mRNA was analyzed by qRT-PCR. ( C ) Immunocytochemistry. Primary SV cells were prepared from 5-week-old mice, cultured, and stained by immunocytochemistry for necdin (red) and CD34, Sca-1, αSMA, or PDGFRβ (green). Double-stained images are merged with nuclear DNA staining (blue) (Merge). Arrowheads point to cells co-expressing necdin and the marker protein. Scale bar, 20 µm.

    Journal: PLoS ONE

    Article Title: Necdin Controls Proliferation of White Adipocyte Progenitor Cells

    doi: 10.1371/journal.pone.0030948

    Figure Lengend Snippet: Necdin is expressed in primary adipose SV cells. ( A ) Western blot analysis of endogenous necdin. WAT stromal-vascular fraction (SVF), adipocyte fraction (AF), pooled WAT (WAT), interscapular BAT (BAT), and brain were prepared from 5-week-old mice. Necdin in the extracts was analyzed by Western blotting. Molecular sizes are in kilodaltons (kDa). ( B ) qRT-PCR for necdin mRNA. Total RNA was extracted from the above fractions and tissues. Necdin mRNA was analyzed by qRT-PCR. ( C ) Immunocytochemistry. Primary SV cells were prepared from 5-week-old mice, cultured, and stained by immunocytochemistry for necdin (red) and CD34, Sca-1, αSMA, or PDGFRβ (green). Double-stained images are merged with nuclear DNA staining (blue) (Merge). Arrowheads point to cells co-expressing necdin and the marker protein. Scale bar, 20 µm.

    Article Snippet: RT-PCR products were quantified using a real-time PCR instrument (LightCycler, Roche) and FastStart DNA MasterPLUS SYBR green I kit (Roche).

    Techniques: Western Blot, Mouse Assay, Quantitative RT-PCR, Immunocytochemistry, Cell Culture, Staining, Expressing, Marker

    Overexpression of MBNL 1‐ AS 1 inhibited the proliferation and induced the apoptosis of BC cells. A, Relative expression of MBNL 1‐ AS 1 in 5637 and T24 cells was assessed by qRT ‐ PCR . B, The viable cells were evaluated using MTT assay in 5637 and T24 cells. C, Flow cytometry analysis was performed to determine the cell cycle distribution of 5637 and T24 cells. D, The percentage of apoptotic cells was detected by flow cytomentry in 5637 and T24 cells. * P

    Journal: Cancer Medicine

    Article Title: Lnc RNA MBNL1‐ AS1 represses cell proliferation and enhances cell apoptosis via targeting miR‐135a‐5p/ PHLPP2/ FOXO1 axis in bladder cancer, et al. Lnc RNA MBNL1‐ AS1 represses cell proliferation and enhances cell apoptosis via targeting miR‐135a‐5p/ PHLPP2/ FOXO1 axis in bladder cancer

    doi: 10.1002/cam4.2684

    Figure Lengend Snippet: Overexpression of MBNL 1‐ AS 1 inhibited the proliferation and induced the apoptosis of BC cells. A, Relative expression of MBNL 1‐ AS 1 in 5637 and T24 cells was assessed by qRT ‐ PCR . B, The viable cells were evaluated using MTT assay in 5637 and T24 cells. C, Flow cytometry analysis was performed to determine the cell cycle distribution of 5637 and T24 cells. D, The percentage of apoptotic cells was detected by flow cytomentry in 5637 and T24 cells. * P

    Article Snippet: 2.6 Quantitative real‐time PCR (q RT ‐ PCR ) Total RNAs were extracted using RNAsimple Total RNA Kit (DP419, TIANGEN) and reverse‐transcribed into cDNA with M‐MLV reverse transcriptase (NG212, TIANGEN). qRT‐PCR was performed using SYBR Green (SY1020, Solarbio) on a real‐time PCR instrument (Exicycler96, BIONEER).

    Techniques: Over Expression, Expressing, Quantitative RT-PCR, MTT Assay, Flow Cytometry