real-time pcr Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher real time pcr stepone real time pcr system
    Effect of esculetin on apoptosis- and cell cycle-related gene expression. The cells were treated with 25 μM, 75 μM, 150 μM, and 300 μM of esculetin for 48 hours, and then targeted <t>mRNA</t> expressions were analyzed by reverse transcription and quantitative real-time <t>PCR.</t> (A) The fold changes of apoptosis-associated genes and (B) cell cycle related genes were plotted on graphs. For each group, two different experiments were performed in triplicate (mean ± SD). * P
    Real Time Pcr Stepone Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr stepone real time pcr system/product/Thermo Fisher
    Average 99 stars, based on 230 article reviews
    Price from $9.99 to $1999.99
    real time pcr stepone real time pcr system - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Bruker Corporation species specific real time pcr
    Effect of esculetin on apoptosis- and cell cycle-related gene expression. The cells were treated with 25 μM, 75 μM, 150 μM, and 300 μM of esculetin for 48 hours, and then targeted <t>mRNA</t> expressions were analyzed by reverse transcription and quantitative real-time <t>PCR.</t> (A) The fold changes of apoptosis-associated genes and (B) cell cycle related genes were plotted on graphs. For each group, two different experiments were performed in triplicate (mean ± SD). * P
    Species Specific Real Time Pcr, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/species specific real time pcr/product/Bruker Corporation
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    species specific real time pcr - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher real time pcr real time pcr
    miR-16, let-7a and miR-34a are increased during mouse ES cell differentiation . Mouse ES cells (Sox1-GFP 46C) were differentiated using an adherent monolayer protocol. Cells at 4 and 8 days were collected for total RNA extraction and subsequently processed for evaluation of specific differentiation markers, as well as proapoptotic miRNA expression by quantitative Real <t>Time-PCR.</t> A positive control for neural differentiation was also performed at day 8 by treating cells with either 10 nM LY411575 or 0.01%DMSO (control) for 12 hours. A) Semi-quantitative RT-PCR analysis for selected markers of lineage commitment in day 4 and 8, as well as in LY411575-treated and untreated cells. B) Representative bright-field, phase contrast images showing increased neuronal differentiation after LY411575 incubation compared with control (DMSO-treated) cells. C) Expression of miR-16, Let-7a and miR-34a at 4 and 8 days of ES cell differentiation and in control (DMSO-treated) and LY411575-treated rosette cultures at 8 days. <t>miRNAs</t> expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA, and GAPDH for normalization. Expression levels were calculated by the ΔΔ C t method using either differentiated cells at 4 days or LY411575-untreated cells as calibrator. Data represent mean ± SEM of three independent experiments. * p
    Real Time Pcr Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 912 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr real time pcr/product/Thermo Fisher
    Average 99 stars, based on 912 article reviews
    Price from $9.99 to $1999.99
    real time pcr real time pcr - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    96
    Thermo Fisher quantitative real time pcr
    KLF15 is the major Kruppel-Like Factor in HPMEC and important regulator of critical endothelial enzymes- Arginase 2 and eNOS Total RNA from Human Pulmonary Microvascular endothelial cells was converted to <t>cDNA</t> and the relative mRNA expression of KLF15 was determined in quiescent HPMEC using real-time <t>PCR</t> (N=3), B) Cell lysates from HPMEC, human coronary artery endothelial cells (HCAEC), Human Aortic Endothelial Cells (HAEC), Human Aortic Smooth Muscle Cells (HASMC) were subjected to western blotting with KLF15, eNOS and β-tubulin antibodies (N=4). Cell lysates from HPMEC overexpressing HA-KLF15 was used as a positive control, C) Total RNA from Human Pulmonary Microvascular endothelial cells was converted to cDNA and the relative mRNA expression of KLF2, 4 and 15 was determined in quiescent HPMEC using real-time PCR (N=3) *p
    Quantitative Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 46127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time pcr/product/Thermo Fisher
    Average 96 stars, based on 46127 article reviews
    Price from $9.99 to $1999.99
    quantitative real time pcr - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    99
    Thermo Fisher realtime pcr
    STING mutants fail to respond to DNA-adduct forming agents. The reconstituted primary Sting −/− MEF cells with empty vector (pBabe), hSTING, R169W, or P203S were treated DMBA (20 μg/ml) or cisplatin (10 μM) for 48 hr. Total RNA was extracted and <t>realtime</t> <t>PCR</t> was performed with the indicated probes after cDNA synthesis. Data shown here are the averages ± SD (n = 3). Asterisks indicate significant difference (p
    Realtime Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/realtime pcr/product/Thermo Fisher
    Average 99 stars, based on 1453 article reviews
    Price from $9.99 to $1999.99
    realtime pcr - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher real time pcr rq pcr
    a Specificity of real-time <t>PCR</t> amplification melting temperature analysis of PCR products recorded in ABI <t>7500</t> Real-time PCR system (Applied Biosystems, Foster City, CA). Descriptions: NC - DNA extracted from non infected SPF chicken kidneys (CEKs). b Specificity NC - DNA extracted from non infected SPF chicken kidneys (CEKs). No real-time PCR curve was observed in the case of the negative control, nor for DNA obtained from other viruses: CAV, HEV, DAdV. Rel-time PCR curve was observed in the case of FAdV infection
    Real Time Pcr Rq Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr rq pcr/product/Thermo Fisher
    Average 99 stars, based on 132 article reviews
    Price from $9.99 to $1999.99
    real time pcr rq pcr - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher real time pcr amplification
    Expression of oppA mRNA transcripts in cultured B. burgdorferi . Expression of the oppA genes was measured by RT <t>PCR</t> (A) and by RPA (B). For RT PCR, RNA was isolated from B. burgdorferi grown in BSK H medium at 37°C. <t>cDNA</t> was generated from total RNA, using gene-specific primers. For each sample, quantitative RT PCR for oppA-I to -V was performed. Determination of copy numbers was calculated by comparison to individual standard curves generated for each primer set. Panel A shows the results of three separate experiments. For RPA, total RNA was hybridized to biotinylated probes specific for each oppA gene and then digested with RNase A-RNase T 1 . Samples were subjected to electrophoresis in a polyacrylamide gel and transferred to nylon membranes. Detection of biotinylated probes was done using chemiluminescence, and quantitation was performed by scanning densitometry. Data shown are individual results from three separate experiments.
    Real Time Pcr Amplification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4779 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr amplification/product/Thermo Fisher
    Average 94 stars, based on 4779 article reviews
    Price from $9.99 to $1999.99
    real time pcr amplification - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    99
    Eppendorf AG real time pcr analysis
    mRNA and protein expression for AR in TG of male and female rats. (A) <t>PCR</t> of TG <t>cDNA</t> produced a single band of the expected size for AR (278 bp, A) in male and female TG. M, marker, NTS, no template control. (B) Bar graphs showing quantitative comparisons
    Real Time Pcr Analysis, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr analysis/product/Eppendorf AG
    Average 99 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    real time pcr analysis - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher real time pcr machine
    The amplification plot of <t>FMDV</t> isolates with classical <t>rRT-PCR</t> reagents and AuNPs-FMDV biosensor: the amplification plot is showing amplification of FMDV isolates with classical rRT-PCR reagents (yellow and violet) curves with CT values 7.03 and 15.9 and with AuNPs-FMDV biosensor (red and blue) curves with CT values 4.09 and 12.93
    Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2466 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr machine/product/Thermo Fisher
    Average 94 stars, based on 2466 article reviews
    Price from $9.99 to $1999.99
    real time pcr machine - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    90
    Thermo Fisher real time pcr thermocycler
    The amplification plot of <t>FMDV</t> isolates with classical <t>rRT-PCR</t> reagents and AuNPs-FMDV biosensor: the amplification plot is showing amplification of FMDV isolates with classical rRT-PCR reagents (yellow and violet) curves with CT values 7.03 and 15.9 and with AuNPs-FMDV biosensor (red and blue) curves with CT values 4.09 and 12.93
    Real Time Pcr Thermocycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr thermocycler/product/Thermo Fisher
    Average 90 stars, based on 186 article reviews
    Price from $9.99 to $1999.99
    real time pcr thermocycler - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    92
    Thermo Fisher fluorescence real time polymerase chain reaction
    Melanocyte activation in the vitamin A-deficient (VAD) pigmented mice. ( A ) The stria vascularis of the middle cochlear turn was analyzed by confocal microscopy. Bright field images show the melanosome (black pigment) distribution. The population of melanocytes in the stria vascularis of the middle cochlear turn of the VAD mice (n = 4) is greater than that of the control mice (n = 4). Scale bar represents 25 µm. ( B ) The findings of <t>real-time</t> <t>polymerase</t> <t>chain</t> <t>reaction</t> analysis reveal the upregulation of tyrosinase in the cochlea of the VAD pigmented mice. Tissues were harvested from the middle cochlear turn of four C57BL/6 mice from each group. *P
    Fluorescence Real Time Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence real time polymerase chain reaction/product/Thermo Fisher
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    fluorescence real time polymerase chain reaction - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Effect of esculetin on apoptosis- and cell cycle-related gene expression. The cells were treated with 25 μM, 75 μM, 150 μM, and 300 μM of esculetin for 48 hours, and then targeted mRNA expressions were analyzed by reverse transcription and quantitative real-time PCR. (A) The fold changes of apoptosis-associated genes and (B) cell cycle related genes were plotted on graphs. For each group, two different experiments were performed in triplicate (mean ± SD). * P

    Journal: Journal of Cancer Prevention

    Article Title: Esculetin Inhibits the Survival of Human Prostate Cancer Cells by Inducing Apoptosis and Arresting the Cell Cycle

    doi: 10.15430/JCP.2018.23.1.10

    Figure Lengend Snippet: Effect of esculetin on apoptosis- and cell cycle-related gene expression. The cells were treated with 25 μM, 75 μM, 150 μM, and 300 μM of esculetin for 48 hours, and then targeted mRNA expressions were analyzed by reverse transcription and quantitative real-time PCR. (A) The fold changes of apoptosis-associated genes and (B) cell cycle related genes were plotted on graphs. For each group, two different experiments were performed in triplicate (mean ± SD). * P

    Article Snippet: Quantitative mRNA expression analyses were achieved on a Real-Time PCR Systems (Applied Biosystems, Foster City, CA, USA) using SYBR Green PCR Master Mix (Thermo Fisher Scientific).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Real time-PCR for GSK3-β; Total RNA was extracted; the standard reverse transcription was performed using RevertAid First Strand cDNA Synthesis Kit. Subsequent real time PCR was done with Power SYBR Green real time master mix and Stepone real time PCR (Applied Biosystems, Carlsbad, U.S.A.). GAPDH primer was used as a housekeeping gene. Each reaction was performed in triplicate. Expression of GSK3-β was down-regulated in non-obstructive azoospermia (3.10±0.19) compared with normal (7.12±0.39) and obstructive azoospermia (6.32±0.42) groups. The difference was significant (p=0.001)

    Journal: Iranian Journal of Reproductive Medicine

    Article Title: Expression of Glycogen synthase kinase 3-? (GSK3-?) gene in azoospermic men

    doi:

    Figure Lengend Snippet: Real time-PCR for GSK3-β; Total RNA was extracted; the standard reverse transcription was performed using RevertAid First Strand cDNA Synthesis Kit. Subsequent real time PCR was done with Power SYBR Green real time master mix and Stepone real time PCR (Applied Biosystems, Carlsbad, U.S.A.). GAPDH primer was used as a housekeeping gene. Each reaction was performed in triplicate. Expression of GSK3-β was down-regulated in non-obstructive azoospermia (3.10±0.19) compared with normal (7.12±0.39) and obstructive azoospermia (6.32±0.42) groups. The difference was significant (p=0.001)

    Article Snippet: Subsequent real time PCR was done with Power Syber Green real time master mix and Stepone real time PCR (Applied biosystems, Carlsbad, USA).

    Techniques: Real-time Polymerase Chain Reaction, SYBR Green Assay, Expressing

    miR-16, let-7a and miR-34a are increased during mouse ES cell differentiation . Mouse ES cells (Sox1-GFP 46C) were differentiated using an adherent monolayer protocol. Cells at 4 and 8 days were collected for total RNA extraction and subsequently processed for evaluation of specific differentiation markers, as well as proapoptotic miRNA expression by quantitative Real Time-PCR. A positive control for neural differentiation was also performed at day 8 by treating cells with either 10 nM LY411575 or 0.01%DMSO (control) for 12 hours. A) Semi-quantitative RT-PCR analysis for selected markers of lineage commitment in day 4 and 8, as well as in LY411575-treated and untreated cells. B) Representative bright-field, phase contrast images showing increased neuronal differentiation after LY411575 incubation compared with control (DMSO-treated) cells. C) Expression of miR-16, Let-7a and miR-34a at 4 and 8 days of ES cell differentiation and in control (DMSO-treated) and LY411575-treated rosette cultures at 8 days. miRNAs expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA, and GAPDH for normalization. Expression levels were calculated by the ΔΔ C t method using either differentiated cells at 4 days or LY411575-untreated cells as calibrator. Data represent mean ± SEM of three independent experiments. * p

    Journal: BMC Genomics

    Article Title: Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation

    doi: 10.1186/1471-2164-11-514

    Figure Lengend Snippet: miR-16, let-7a and miR-34a are increased during mouse ES cell differentiation . Mouse ES cells (Sox1-GFP 46C) were differentiated using an adherent monolayer protocol. Cells at 4 and 8 days were collected for total RNA extraction and subsequently processed for evaluation of specific differentiation markers, as well as proapoptotic miRNA expression by quantitative Real Time-PCR. A positive control for neural differentiation was also performed at day 8 by treating cells with either 10 nM LY411575 or 0.01%DMSO (control) for 12 hours. A) Semi-quantitative RT-PCR analysis for selected markers of lineage commitment in day 4 and 8, as well as in LY411575-treated and untreated cells. B) Representative bright-field, phase contrast images showing increased neuronal differentiation after LY411575 incubation compared with control (DMSO-treated) cells. C) Expression of miR-16, Let-7a and miR-34a at 4 and 8 days of ES cell differentiation and in control (DMSO-treated) and LY411575-treated rosette cultures at 8 days. miRNAs expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA, and GAPDH for normalization. Expression levels were calculated by the ΔΔ C t method using either differentiated cells at 4 days or LY411575-untreated cells as calibrator. Data represent mean ± SEM of three independent experiments. * p

    Article Snippet: Evaluation of miRNAs expression levels by quantitative Real Time-PCR Real-time PCR was performed in an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Techniques: Cell Differentiation, RNA Extraction, Expressing, Real-time Polymerase Chain Reaction, Positive Control, Quantitative RT-PCR, Incubation

    Apoptosis-associated miRNAs are modulated during mouse NS cell differentiation . Expression of specific proapoptotic (miR-16, let-7a and miR-34a) and antiapoptotic miRNAs (miR-20a and miR-19a) were analyzed by quantitative Real Time-PCR from 10 ng of total RNA using specific Taqman primers and GAPDH for normalization. * p

    Journal: BMC Genomics

    Article Title: Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation

    doi: 10.1186/1471-2164-11-514

    Figure Lengend Snippet: Apoptosis-associated miRNAs are modulated during mouse NS cell differentiation . Expression of specific proapoptotic (miR-16, let-7a and miR-34a) and antiapoptotic miRNAs (miR-20a and miR-19a) were analyzed by quantitative Real Time-PCR from 10 ng of total RNA using specific Taqman primers and GAPDH for normalization. * p

    Article Snippet: Evaluation of miRNAs expression levels by quantitative Real Time-PCR Real-time PCR was performed in an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Techniques: Cell Differentiation, Expressing, Real-time Polymerase Chain Reaction

    Inhibition of apoptosis by TUDCA was not associated with a decrease in proapoptotic miRNAs expression . Mouse NS cells with 3 days of differentiation were either untreated or treated with 50 μM of TUDCA for 72 hours. Collected cells were stained with Annexin-V-APC/PI to evaluate cell death, or processed for total RNA extraction and miRNAs expression evaluation by quantitative Real Time-PCR. A) Representative Annexin V-APC/PI stainings showing decreased cell death after TUDCA incubation. B) Quantitation of either dying (Annexin+/PI-) or dead (Annexin+/PI+) cells depicted in FACS diagrams. Results are mean ± SEM of triplicates. C) Expression of proapoptotic miRNAs at 3 and 6 days, with or without TUDCA treatment. miR-16, let-7a and miR-34a expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA, and GAPDH for normalization. Expression levels were calculated by the ΔΔ C t method using differentiated cells at 3 days as calibrator. Data represent mean ± SEM of three independent experiments. * p

    Journal: BMC Genomics

    Article Title: Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation

    doi: 10.1186/1471-2164-11-514

    Figure Lengend Snippet: Inhibition of apoptosis by TUDCA was not associated with a decrease in proapoptotic miRNAs expression . Mouse NS cells with 3 days of differentiation were either untreated or treated with 50 μM of TUDCA for 72 hours. Collected cells were stained with Annexin-V-APC/PI to evaluate cell death, or processed for total RNA extraction and miRNAs expression evaluation by quantitative Real Time-PCR. A) Representative Annexin V-APC/PI stainings showing decreased cell death after TUDCA incubation. B) Quantitation of either dying (Annexin+/PI-) or dead (Annexin+/PI+) cells depicted in FACS diagrams. Results are mean ± SEM of triplicates. C) Expression of proapoptotic miRNAs at 3 and 6 days, with or without TUDCA treatment. miR-16, let-7a and miR-34a expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA, and GAPDH for normalization. Expression levels were calculated by the ΔΔ C t method using differentiated cells at 3 days as calibrator. Data represent mean ± SEM of three independent experiments. * p

    Article Snippet: Evaluation of miRNAs expression levels by quantitative Real Time-PCR Real-time PCR was performed in an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Techniques: Inhibition, Expressing, Staining, RNA Extraction, Real-time Polymerase Chain Reaction, Incubation, Quantitation Assay, FACS

    Differentiation of PC12 and NT2N cells were associated with modulated levels of miR-16, let-7a and miR-34a expression . PC12 cells were differentiated upon NGF treatment at 1 day with either 5 or 50 ng/ml. NGF-untreated cells were also prepared. Cells were collected at 1, 2 4 and 7 days. NT2N cells were induced to differentiate with 10 μM all-trans retinoic acid and collected at 0, 14 and 21 days. In parallel experiments, cells were replated at 14 days in Matrigel, deprived of retinoic acid and incubated with mitotic inhibitors and further cultured for 7 days. Control cells were maintained in initial retinoic acid treatment. Both PC12 and NT2N-collected cells were processed for total RNA extraction and evaluation of miRNAs expression levels using quantitative Real Time-PCR. A and B) miR-16, let-7a and miR-34a expression during PC12 and NT2N differentiation, respectively. miRNAs expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA. GAPDH and RNU6B were used for normalization in PC12 and NT2N cells, respectively. Expression levels were calculated by the ΔΔ C t method. NGF-untreated cells at 1 day was used as calibrator in PC12 cells, while undifferentiated (day 0) or unreplated cells were used as calibrator in NT2N cells. Data represent mean ± SEM of three independent experiments. A) * p

    Journal: BMC Genomics

    Article Title: Apoptosis-associated microRNAs are modulated in mouse, rat and human neural differentiation

    doi: 10.1186/1471-2164-11-514

    Figure Lengend Snippet: Differentiation of PC12 and NT2N cells were associated with modulated levels of miR-16, let-7a and miR-34a expression . PC12 cells were differentiated upon NGF treatment at 1 day with either 5 or 50 ng/ml. NGF-untreated cells were also prepared. Cells were collected at 1, 2 4 and 7 days. NT2N cells were induced to differentiate with 10 μM all-trans retinoic acid and collected at 0, 14 and 21 days. In parallel experiments, cells were replated at 14 days in Matrigel, deprived of retinoic acid and incubated with mitotic inhibitors and further cultured for 7 days. Control cells were maintained in initial retinoic acid treatment. Both PC12 and NT2N-collected cells were processed for total RNA extraction and evaluation of miRNAs expression levels using quantitative Real Time-PCR. A and B) miR-16, let-7a and miR-34a expression during PC12 and NT2N differentiation, respectively. miRNAs expression were evaluated from 10 ng of total RNA, using specific primers for each miRNA. GAPDH and RNU6B were used for normalization in PC12 and NT2N cells, respectively. Expression levels were calculated by the ΔΔ C t method. NGF-untreated cells at 1 day was used as calibrator in PC12 cells, while undifferentiated (day 0) or unreplated cells were used as calibrator in NT2N cells. Data represent mean ± SEM of three independent experiments. A) * p

    Article Snippet: Evaluation of miRNAs expression levels by quantitative Real Time-PCR Real-time PCR was performed in an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems, Foster City, CA).

    Techniques: Expressing, Incubation, Cell Culture, RNA Extraction, Real-time Polymerase Chain Reaction

    KLF15 is the major Kruppel-Like Factor in HPMEC and important regulator of critical endothelial enzymes- Arginase 2 and eNOS Total RNA from Human Pulmonary Microvascular endothelial cells was converted to cDNA and the relative mRNA expression of KLF15 was determined in quiescent HPMEC using real-time PCR (N=3), B) Cell lysates from HPMEC, human coronary artery endothelial cells (HCAEC), Human Aortic Endothelial Cells (HAEC), Human Aortic Smooth Muscle Cells (HASMC) were subjected to western blotting with KLF15, eNOS and β-tubulin antibodies (N=4). Cell lysates from HPMEC overexpressing HA-KLF15 was used as a positive control, C) Total RNA from Human Pulmonary Microvascular endothelial cells was converted to cDNA and the relative mRNA expression of KLF2, 4 and 15 was determined in quiescent HPMEC using real-time PCR (N=3) *p

    Journal: Arteriosclerosis, thrombosis, and vascular biology

    Article Title: Hypoxia Triggers SENP1 Modulation of Kruppel-Like Factor 15 and Transcriptional Regulation of Arginase 2 in Pulmonary Endothelium

    doi: 10.1161/ATVBAHA.117.310660

    Figure Lengend Snippet: KLF15 is the major Kruppel-Like Factor in HPMEC and important regulator of critical endothelial enzymes- Arginase 2 and eNOS Total RNA from Human Pulmonary Microvascular endothelial cells was converted to cDNA and the relative mRNA expression of KLF15 was determined in quiescent HPMEC using real-time PCR (N=3), B) Cell lysates from HPMEC, human coronary artery endothelial cells (HCAEC), Human Aortic Endothelial Cells (HAEC), Human Aortic Smooth Muscle Cells (HASMC) were subjected to western blotting with KLF15, eNOS and β-tubulin antibodies (N=4). Cell lysates from HPMEC overexpressing HA-KLF15 was used as a positive control, C) Total RNA from Human Pulmonary Microvascular endothelial cells was converted to cDNA and the relative mRNA expression of KLF2, 4 and 15 was determined in quiescent HPMEC using real-time PCR (N=3) *p

    Article Snippet: RNA was then reverse transcribed with oligo dT primers to obtain cDNA and quantitative real time PCR (Applied Biosystems) was performed using SYBR Green Supermix mix (Applied Biosystems) whereas semi-quantitative RTPCR was performed using a conventional Biorad PCR machine and the following primer sets: Human Arg2 : Forward: 5′-GGG CCC TGA AGG CTG TAG-3′, Reverse: 5′-AAT GGA GCC ACT GCC ATC-3′.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Positive Control

    miR-21 is overexpressed in high-grade intraepithelial neoplasia (HG-IEN) and Barrett's adenocarcinoma (BAc). (A) Predicted base complementarity of miR-21 to 3′-UTR binding site of PDCD4 , as predicted by the Sanger miR-database (miRBase, http://www.mirbase.org/ ). (B) Altered miR-21 expression between cancerous tissue (CT; HG-IEN and BAc) and non-cancerous tissue (NCT) in BAc patients by qRT-PCR analysis. miR-21 was significantly upregulated in HG-IEN and BAc samples (p

    Journal: Journal of Clinical Pathology

    Article Title: Programmed cell death 4 (PDCD4) expression during multistep Barrett's carcinogenesis

    doi: 10.1136/jcp.2010.078253

    Figure Lengend Snippet: miR-21 is overexpressed in high-grade intraepithelial neoplasia (HG-IEN) and Barrett's adenocarcinoma (BAc). (A) Predicted base complementarity of miR-21 to 3′-UTR binding site of PDCD4 , as predicted by the Sanger miR-database (miRBase, http://www.mirbase.org/ ). (B) Altered miR-21 expression between cancerous tissue (CT; HG-IEN and BAc) and non-cancerous tissue (NCT) in BAc patients by qRT-PCR analysis. miR-21 was significantly upregulated in HG-IEN and BAc samples (p

    Article Snippet: The NCodeTM miRNA qRT-PCR method (Invitrogen, Carlsbad, California, USA) was used to detect and quantify mature miR-21 (primer sequence: 5′-CGGTAGCTTATCAGACTGATGTTGA-3′) on real-time PCR instruments according to the manufacturer's instructions (Applied Biosystems, Foster City, California, USA).

    Techniques: BAC Assay, Binding Assay, Expressing, Quantitative RT-PCR

    STING mutants fail to respond to DNA-adduct forming agents. The reconstituted primary Sting −/− MEF cells with empty vector (pBabe), hSTING, R169W, or P203S were treated DMBA (20 μg/ml) or cisplatin (10 μM) for 48 hr. Total RNA was extracted and realtime PCR was performed with the indicated probes after cDNA synthesis. Data shown here are the averages ± SD (n = 3). Asterisks indicate significant difference (p

    Journal: Oncogene

    Article Title: Suppression of STING Signaling through Epigenetic Silencing and Missense Mutation Impedes DNA-Damage Mediated Cytokine Production

    doi: 10.1038/s41388-017-0120-0

    Figure Lengend Snippet: STING mutants fail to respond to DNA-adduct forming agents. The reconstituted primary Sting −/− MEF cells with empty vector (pBabe), hSTING, R169W, or P203S were treated DMBA (20 μg/ml) or cisplatin (10 μM) for 48 hr. Total RNA was extracted and realtime PCR was performed with the indicated probes after cDNA synthesis. Data shown here are the averages ± SD (n = 3). Asterisks indicate significant difference (p

    Article Snippet: Realtime PCR was performed using StepOnePlus Real-Time PCR system (Applied Biosystems).

    Techniques: Plasmid Preparation, Polymerase Chain Reaction

    Effect of the removal of the histone N-terminal tails on the thermal stabilities of the nucleosomes. Each nucleosome, containing tlH2A, tlH2B, tlH3, or tlH4 (final concentration, 2.25 μM), was mixed with SYPRO Orange, and the sample temperature was increased by the StepOnePlus™ Real-Time PCR unit (Applied Biosystems). The temperature gradient was from 25 to 95 °C, in steps of 1 °C/min. Fluorescence signals of SYPRO Orange bound to the denatured histones were measured, and the thermal denaturing curves of the wt, tlH2A, tlH2B, tlH3, and tlH4 nucleosomes are shown in black, yellow, pink, blue, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: FEBS Open Bio

    Article Title: Contribution of histone N-terminal tails to the structure and stability of nucleosomes

    doi: 10.1016/j.fob.2013.08.007

    Figure Lengend Snippet: Effect of the removal of the histone N-terminal tails on the thermal stabilities of the nucleosomes. Each nucleosome, containing tlH2A, tlH2B, tlH3, or tlH4 (final concentration, 2.25 μM), was mixed with SYPRO Orange, and the sample temperature was increased by the StepOnePlus™ Real-Time PCR unit (Applied Biosystems). The temperature gradient was from 25 to 95 °C, in steps of 1 °C/min. Fluorescence signals of SYPRO Orange bound to the denatured histones were measured, and the thermal denaturing curves of the wt, tlH2A, tlH2B, tlH3, and tlH4 nucleosomes are shown in black, yellow, pink, blue, and green, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: The sample temperature was increased by the StepOnePlus™ Real-Time PCR unit (Applied Biosystems), and the fluorescence signals were measured with this system.

    Techniques: Concentration Assay, Real-time Polymerase Chain Reaction, Fluorescence

    a Specificity of real-time PCR amplification melting temperature analysis of PCR products recorded in ABI 7500 Real-time PCR system (Applied Biosystems, Foster City, CA). Descriptions: NC - DNA extracted from non infected SPF chicken kidneys (CEKs). b Specificity NC - DNA extracted from non infected SPF chicken kidneys (CEKs). No real-time PCR curve was observed in the case of the negative control, nor for DNA obtained from other viruses: CAV, HEV, DAdV. Rel-time PCR curve was observed in the case of FAdV infection

    Journal: BMC Veterinary Research

    Article Title: Detection of fowl adenovirus D strains in wild birds in Poland by Loop-Mediated Isothermal Amplification (LAMP)

    doi: 10.1186/s12917-020-2271-4

    Figure Lengend Snippet: a Specificity of real-time PCR amplification melting temperature analysis of PCR products recorded in ABI 7500 Real-time PCR system (Applied Biosystems, Foster City, CA). Descriptions: NC - DNA extracted from non infected SPF chicken kidneys (CEKs). b Specificity NC - DNA extracted from non infected SPF chicken kidneys (CEKs). No real-time PCR curve was observed in the case of the negative control, nor for DNA obtained from other viruses: CAV, HEV, DAdV. Rel-time PCR curve was observed in the case of FAdV infection

    Article Snippet: The real-time PCR have been performed by using Applied Biosystems 7500 Real-time PCR, and were used in a final volume of 25 μl.

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction, Infection, Negative Control

    Transient expression of AtHPT, AtTC and AtTC + AtHPT post agroinfiltration by reverse transcription PCR. Top panel: Target genes Lane 1 Wild type control Lane 2 HPT Lane 3 TC Lane 3 HPT + TC with TC primers Lane 3 HPT + TC with HPT primers Lane 6 HPT + TC with both HPT TC primers. Bottom panel: Reference gene F-box

    Journal: 3 Biotech

    Article Title: Rapid enhancement of α-tocopherol content in Nicotiana benthamiana by transient expression of Arabidopsis thaliana Tocopherol cyclase and Homogentisate phytyl transferase genes

    doi: 10.1007/s13205-018-1496-4

    Figure Lengend Snippet: Transient expression of AtHPT, AtTC and AtTC + AtHPT post agroinfiltration by reverse transcription PCR. Top panel: Target genes Lane 1 Wild type control Lane 2 HPT Lane 3 TC Lane 3 HPT + TC with TC primers Lane 3 HPT + TC with HPT primers Lane 6 HPT + TC with both HPT TC primers. Bottom panel: Reference gene F-box

    Article Snippet: Quantitive gene expression of both HPT and TC was analysed using Real-Time PCR system (Applied Biosystems, USA).

    Techniques: Expressing, Polymerase Chain Reaction

    Expression of oppA mRNA transcripts in cultured B. burgdorferi . Expression of the oppA genes was measured by RT PCR (A) and by RPA (B). For RT PCR, RNA was isolated from B. burgdorferi grown in BSK H medium at 37°C. cDNA was generated from total RNA, using gene-specific primers. For each sample, quantitative RT PCR for oppA-I to -V was performed. Determination of copy numbers was calculated by comparison to individual standard curves generated for each primer set. Panel A shows the results of three separate experiments. For RPA, total RNA was hybridized to biotinylated probes specific for each oppA gene and then digested with RNase A-RNase T 1 . Samples were subjected to electrophoresis in a polyacrylamide gel and transferred to nylon membranes. Detection of biotinylated probes was done using chemiluminescence, and quantitation was performed by scanning densitometry. Data shown are individual results from three separate experiments.

    Journal: Journal of Bacteriology

    Article Title: Effects of Environmental Changes on Expression of the Oligopeptide Permease (opp) Genes of Borrelia burgdorferi

    doi: 10.1128/JB.184.22.6198-6206.2002

    Figure Lengend Snippet: Expression of oppA mRNA transcripts in cultured B. burgdorferi . Expression of the oppA genes was measured by RT PCR (A) and by RPA (B). For RT PCR, RNA was isolated from B. burgdorferi grown in BSK H medium at 37°C. cDNA was generated from total RNA, using gene-specific primers. For each sample, quantitative RT PCR for oppA-I to -V was performed. Determination of copy numbers was calculated by comparison to individual standard curves generated for each primer set. Panel A shows the results of three separate experiments. For RPA, total RNA was hybridized to biotinylated probes specific for each oppA gene and then digested with RNase A-RNase T 1 . Samples were subjected to electrophoresis in a polyacrylamide gel and transferred to nylon membranes. Detection of biotinylated probes was done using chemiluminescence, and quantitation was performed by scanning densitometry. Data shown are individual results from three separate experiments.

    Article Snippet: The generated cDNA was used as a template for real-time PCR amplification (ABI 7700; Applied Biosystems), using SYBR green fluorescent dye (SYBR Green Master Mix; Applied Biosystems) and specific primers for each oppA gene.

    Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Recombinase Polymerase Amplification, Isolation, Generated, Quantitative RT-PCR, Electrophoresis, Quantitation Assay

    mRNA and protein expression for AR in TG of male and female rats. (A) PCR of TG cDNA produced a single band of the expected size for AR (278 bp, A) in male and female TG. M, marker, NTS, no template control. (B) Bar graphs showing quantitative comparisons

    Journal: Neuroscience

    Article Title: THE ROLE OF ANDROGEN RECEPTOR IN TRANSCRIPTIONAL MODULATION OF CANNABINOID RECEPTOR TYPE 1 GENE IN RAT TRIGEMINAL GANGLIA

    doi: 10.1016/j.neuroscience.2013.09.014

    Figure Lengend Snippet: mRNA and protein expression for AR in TG of male and female rats. (A) PCR of TG cDNA produced a single band of the expected size for AR (278 bp, A) in male and female TG. M, marker, NTS, no template control. (B) Bar graphs showing quantitative comparisons

    Article Snippet: To assess cytokine-induced changes in CB1 mRNA level, and to compare AR and IL-1β receptor (IL-1R) expression between male and female TG, real-time PCR analysis of cDNA equal to 25 ng RNA was performed on the Eppendorf Mastercycler ep realplex 2.0.

    Techniques: Expressing, Polymerase Chain Reaction, Produced, Marker

    The amplification plot of FMDV isolates with classical rRT-PCR reagents and AuNPs-FMDV biosensor: the amplification plot is showing amplification of FMDV isolates with classical rRT-PCR reagents (yellow and violet) curves with CT values 7.03 and 15.9 and with AuNPs-FMDV biosensor (red and blue) curves with CT values 4.09 and 12.93

    Journal: Journal of Nanobiotechnology

    Article Title: Development of gold nanoparticles biosensor for ultrasensitive diagnosis of foot and mouth disease virus

    doi: 10.1186/s12951-018-0374-x

    Figure Lengend Snippet: The amplification plot of FMDV isolates with classical rRT-PCR reagents and AuNPs-FMDV biosensor: the amplification plot is showing amplification of FMDV isolates with classical rRT-PCR reagents (yellow and violet) curves with CT values 7.03 and 15.9 and with AuNPs-FMDV biosensor (red and blue) curves with CT values 4.09 and 12.93

    Article Snippet: FMDV rRT-PCR was done using QuantiTect Kit (Qiagen) in a real-time PCR machine (StepOne, Applied Biosystems) with a thermal profile according to the manufacturer’s instructions.

    Techniques: Amplification, Quantitative RT-PCR

    Melanocyte activation in the vitamin A-deficient (VAD) pigmented mice. ( A ) The stria vascularis of the middle cochlear turn was analyzed by confocal microscopy. Bright field images show the melanosome (black pigment) distribution. The population of melanocytes in the stria vascularis of the middle cochlear turn of the VAD mice (n = 4) is greater than that of the control mice (n = 4). Scale bar represents 25 µm. ( B ) The findings of real-time polymerase chain reaction analysis reveal the upregulation of tyrosinase in the cochlea of the VAD pigmented mice. Tissues were harvested from the middle cochlear turn of four C57BL/6 mice from each group. *P

    Journal: Scientific Reports

    Article Title: Progressive hearing loss in vitamin A-deficient mice which may be protected by the activation of cochlear melanocyte

    doi: 10.1038/s41598-018-34653-8

    Figure Lengend Snippet: Melanocyte activation in the vitamin A-deficient (VAD) pigmented mice. ( A ) The stria vascularis of the middle cochlear turn was analyzed by confocal microscopy. Bright field images show the melanosome (black pigment) distribution. The population of melanocytes in the stria vascularis of the middle cochlear turn of the VAD mice (n = 4) is greater than that of the control mice (n = 4). Scale bar represents 25 µm. ( B ) The findings of real-time polymerase chain reaction analysis reveal the upregulation of tyrosinase in the cochlea of the VAD pigmented mice. Tissues were harvested from the middle cochlear turn of four C57BL/6 mice from each group. *P

    Article Snippet: For quantification of gene expression, fluorescence real-time polymerase chain reaction (ABI PRISM 7700; PE Applied Biosystems) was performed according to the manufacturer’s instructions by using the double-stranded DNA dye SYBR Green (Perkin-Elmer, Boston, MA, USA).

    Techniques: Activation Assay, Mouse Assay, Confocal Microscopy, Real-time Polymerase Chain Reaction