real-time pcr Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Thermo Fisher steponeplus real time pcr system
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Steponeplus Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 39878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/steponeplus real time pcr system/product/Thermo Fisher
    Average 90 stars, based on 39878 article reviews
    Price from $9.99 to $1999.99
    steponeplus real time pcr system - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    85
    Bruker Corporation real time pcr system
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Real Time Pcr System, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr system/product/Bruker Corporation
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    real time pcr system - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    93
    Stratagene real time pcr analysis real time pcr
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Real Time Pcr Analysis Real Time Pcr, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr analysis real time pcr/product/Stratagene
    Average 93 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    real time pcr analysis real time pcr - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher real time pcr quantitative real time pcr
    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by <t>qRT-PCR</t> on a <t>StepOnePlus</t> real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P
    Real Time Pcr Quantitative Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr quantitative real time pcr/product/Thermo Fisher
    Average 99 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    real time pcr quantitative real time pcr - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher stepone plus rt pcr system
    Effect of fucoxanthin rich powder on mRNA expression of transcription factor and enzymes related to antioxidant system in the liver of rats. Total RNA was isolated using TRI-reagenet, and cDNA was synthesized using 3 ug of total RNA with SuperScript II reverse transcriptase. Realtime <t>PCR</t> was performed using SYBR green and standard procedures to assess the mRNA expression of the primer in liver samples obtained from each group. An Applied Biosystem <t>StepOne</t> softwere v2.1 was used. Each bar represents the mean ± S.E of three independent experiments. Different letters above each bar indicate significant differences among the groups at α = 0.05 as determined by Duncan's multiple range tests. HO-1: heme oxygenase (decycling) 1, Nrf2: nuclear factor, erythroid derived 2, like 2, NQO-1: NAD(P)H quinone oxidoreductase 1.
    Stepone Plus Rt Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 386 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stepone plus rt pcr system/product/Thermo Fisher
    Average 90 stars, based on 386 article reviews
    Price from $9.99 to $1999.99
    stepone plus rt pcr system - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher real time pcr system
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 16906 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr system/product/Thermo Fisher
    Average 90 stars, based on 16906 article reviews
    Price from $9.99 to $1999.99
    real time pcr system - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    93
    Kaneka Corp real time pcr
    <t>Prox1</t> is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by <t>PCR.</t> Data represent one of three separate experiments.
    Real Time Pcr, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 93/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr/product/Kaneka Corp
    Average 93 stars, based on 218 article reviews
    Price from $9.99 to $1999.99
    real time pcr - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    90
    Thermo Fisher real time pcr systems
    The tissue RARRES2 gene expression level is down-regulated in ACC but is not associated with patient prognosis. A, <t>TaqMan</t> real-time quantitative <t>PCR</t> reveals down-regulation of the RARRES2 gene expression level in our cohort of ACC samples as compared
    Real Time Pcr Systems, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 649 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr systems/product/Thermo Fisher
    Average 90 stars, based on 649 article reviews
    Price from $9.99 to $1999.99
    real time pcr systems - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Eppendorf AG adhesive masterclear real time pcr film
    The tissue RARRES2 gene expression level is down-regulated in ACC but is not associated with patient prognosis. A, <t>TaqMan</t> real-time quantitative <t>PCR</t> reveals down-regulation of the RARRES2 gene expression level in our cohort of ACC samples as compared
    Adhesive Masterclear Real Time Pcr Film, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adhesive masterclear real time pcr film/product/Eppendorf AG
    Average 90 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    adhesive masterclear real time pcr film - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher fast real time pcr system
    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative <t>PCR</t> (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P
    Fast Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast real time pcr system/product/Thermo Fisher
    Average 90 stars, based on 2672 article reviews
    Price from $9.99 to $1999.99
    fast real time pcr system - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher viia 7 real time pcr system
    Platform comparison. Allelic discrimination plots of the four GBA mutations. Water was used as the no template control. Fig. 1A: Validation on the ABI PRISM® 7900 HT Sequence Detection System. Fig. 1B: Validation on the Applied Biosystems ViiA™ 7 Real-Time <t>PCR</t> System. Fig. 1C: Blind validation on the ABI PRISM® 7900 HT Sequence Detection System.
    Viia 7 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viia 7 real time pcr system/product/Thermo Fisher
    Average 90 stars, based on 10752 article reviews
    Price from $9.99 to $1999.99
    viia 7 real time pcr system - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Eppendorf AG real time pcr tube strips
    Platform comparison. Allelic discrimination plots of the four GBA mutations. Water was used as the no template control. Fig. 1A: Validation on the ABI PRISM® 7900 HT Sequence Detection System. Fig. 1B: Validation on the Applied Biosystems ViiA™ 7 Real-Time <t>PCR</t> System. Fig. 1C: Blind validation on the ABI PRISM® 7900 HT Sequence Detection System.
    Real Time Pcr Tube Strips, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr tube strips/product/Eppendorf AG
    Average 90 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    real time pcr tube strips - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Bio-Rad miniopticon real time pcr detection system
    Real-time quantitative <t>PCR</t> analysis confirmed cercariae gender-associated DNA microarray results. PCR was performed using a <t>MiniOpticon</t> Real-Time PCR Detection System (Bio-Rad, UK) and SYBR Green I chemistry according to manufacturer's instructions. Briefly, real-time PCR parameters included 40 cycles, fluorescent reading after each cycle and melt curve analysis of individual products at the end of the 40 cycles. Unique oligonucleotide identification number is shown along with BLASTx annotation and gender association. Significant similarity is defined as (E) value≤10 −05 . Fold-difference was calculated as described in Methods . Primer characteristics (Tm, annealing temperature and sequence) along with amplicon size are included in the Dataset S1 (RT-PCR Primers). Underlined unique IDs represent gender-associated transcripts identified by the indirect hybridization strategy (parasite cDNA versus parasite gDNA). The italicized unique ID represents a gender-associated transcript identified by the direct hybridization strategy (female cDNA versus male cDNA). Plain text unique IDs represent gender-associated transcripts identified by both hybridization strategies.
    Miniopticon Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/miniopticon real time pcr detection system/product/Bio-Rad
    Average 90 stars, based on 1115 article reviews
    Price from $9.99 to $1999.99
    miniopticon real time pcr detection system - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    78
    Bioneer Corporation real time pcr accupower mp real time pcr kit
    Real-time quantitative <t>PCR</t> analysis confirmed cercariae gender-associated DNA microarray results. PCR was performed using a <t>MiniOpticon</t> Real-Time PCR Detection System (Bio-Rad, UK) and SYBR Green I chemistry according to manufacturer's instructions. Briefly, real-time PCR parameters included 40 cycles, fluorescent reading after each cycle and melt curve analysis of individual products at the end of the 40 cycles. Unique oligonucleotide identification number is shown along with BLASTx annotation and gender association. Significant similarity is defined as (E) value≤10 −05 . Fold-difference was calculated as described in Methods . Primer characteristics (Tm, annealing temperature and sequence) along with amplicon size are included in the Dataset S1 (RT-PCR Primers). Underlined unique IDs represent gender-associated transcripts identified by the indirect hybridization strategy (parasite cDNA versus parasite gDNA). The italicized unique ID represents a gender-associated transcript identified by the direct hybridization strategy (female cDNA versus male cDNA). Plain text unique IDs represent gender-associated transcripts identified by both hybridization strategies.
    Real Time Pcr Accupower Mp Real Time Pcr Kit, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr accupower mp real time pcr kit/product/Bioneer Corporation
    Average 78 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    real time pcr accupower mp real time pcr kit - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    78
    Tellgen Corporation multiplex real time pcr analysis multiplex real time pcr
    Real-time quantitative <t>PCR</t> analysis confirmed cercariae gender-associated DNA microarray results. PCR was performed using a <t>MiniOpticon</t> Real-Time PCR Detection System (Bio-Rad, UK) and SYBR Green I chemistry according to manufacturer's instructions. Briefly, real-time PCR parameters included 40 cycles, fluorescent reading after each cycle and melt curve analysis of individual products at the end of the 40 cycles. Unique oligonucleotide identification number is shown along with BLASTx annotation and gender association. Significant similarity is defined as (E) value≤10 −05 . Fold-difference was calculated as described in Methods . Primer characteristics (Tm, annealing temperature and sequence) along with amplicon size are included in the Dataset S1 (RT-PCR Primers). Underlined unique IDs represent gender-associated transcripts identified by the indirect hybridization strategy (parasite cDNA versus parasite gDNA). The italicized unique ID represents a gender-associated transcript identified by the direct hybridization strategy (female cDNA versus male cDNA). Plain text unique IDs represent gender-associated transcripts identified by both hybridization strategies.
    Multiplex Real Time Pcr Analysis Multiplex Real Time Pcr, supplied by Tellgen Corporation, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex real time pcr analysis multiplex real time pcr/product/Tellgen Corporation
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    multiplex real time pcr analysis multiplex real time pcr - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    79
    Sigma-Genosys quantitative real time pcr quantitative real time pcr primers
    Real-time quantitative <t>PCR</t> analysis confirmed cercariae gender-associated DNA microarray results. PCR was performed using a <t>MiniOpticon</t> Real-Time PCR Detection System (Bio-Rad, UK) and SYBR Green I chemistry according to manufacturer's instructions. Briefly, real-time PCR parameters included 40 cycles, fluorescent reading after each cycle and melt curve analysis of individual products at the end of the 40 cycles. Unique oligonucleotide identification number is shown along with BLASTx annotation and gender association. Significant similarity is defined as (E) value≤10 −05 . Fold-difference was calculated as described in Methods . Primer characteristics (Tm, annealing temperature and sequence) along with amplicon size are included in the Dataset S1 (RT-PCR Primers). Underlined unique IDs represent gender-associated transcripts identified by the indirect hybridization strategy (parasite cDNA versus parasite gDNA). The italicized unique ID represents a gender-associated transcript identified by the direct hybridization strategy (female cDNA versus male cDNA). Plain text unique IDs represent gender-associated transcripts identified by both hybridization strategies.
    Quantitative Real Time Pcr Quantitative Real Time Pcr Primers, supplied by Sigma-Genosys, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative real time pcr quantitative real time pcr primers/product/Sigma-Genosys
    Average 79 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    quantitative real time pcr quantitative real time pcr primers - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    77
    Thermo Fisher real time pcr sybr green based real time pcr
    Real-time quantitative <t>PCR</t> analysis confirmed cercariae gender-associated DNA microarray results. PCR was performed using a <t>MiniOpticon</t> Real-Time PCR Detection System (Bio-Rad, UK) and SYBR Green I chemistry according to manufacturer's instructions. Briefly, real-time PCR parameters included 40 cycles, fluorescent reading after each cycle and melt curve analysis of individual products at the end of the 40 cycles. Unique oligonucleotide identification number is shown along with BLASTx annotation and gender association. Significant similarity is defined as (E) value≤10 −05 . Fold-difference was calculated as described in Methods . Primer characteristics (Tm, annealing temperature and sequence) along with amplicon size are included in the Dataset S1 (RT-PCR Primers). Underlined unique IDs represent gender-associated transcripts identified by the indirect hybridization strategy (parasite cDNA versus parasite gDNA). The italicized unique ID represents a gender-associated transcript identified by the direct hybridization strategy (female cDNA versus male cDNA). Plain text unique IDs represent gender-associated transcripts identified by both hybridization strategies.
    Real Time Pcr Sybr Green Based Real Time Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr sybr green based real time pcr/product/Thermo Fisher
    Average 77 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    real time pcr sybr green based real time pcr - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    78
    Bio-Rad real time qrt pcr real time qrt pcr
    Relative expression of P. eryngii KNR2312 receptors (a) and pheromones (b) in the monokaryon (P6) and dikaryon (KNR2312), as determined by real-time <t>qRT-PCR.</t> Gene expression was normalized to 18S rRNA expression and calibrated to the value for the monokaryon (P6), which was assigned a value of 1, by the standard curve method (Bio-Rad). All assays were performed in triplicate. The error bars show the standard deviations for triplicate samples.
    Real Time Qrt Pcr Real Time Qrt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time qrt pcr real time qrt pcr/product/Bio-Rad
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    real time qrt pcr real time qrt pcr - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    79
    Roche real time pcr real time pcr master mix
    Relative expression of P. eryngii KNR2312 receptors (a) and pheromones (b) in the monokaryon (P6) and dikaryon (KNR2312), as determined by real-time <t>qRT-PCR.</t> Gene expression was normalized to 18S rRNA expression and calibrated to the value for the monokaryon (P6), which was assigned a value of 1, by the standard curve method (Bio-Rad). All assays were performed in triplicate. The error bars show the standard deviations for triplicate samples.
    Real Time Pcr Real Time Pcr Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr real time pcr master mix/product/Roche
    Average 79 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    real time pcr real time pcr master mix - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    90
    Thermo Fisher quantstudio dx
    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the <t>QuantStudio</t> Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)
    Quantstudio Dx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantstudio dx/product/Thermo Fisher
    Average 90 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    quantstudio dx - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    88
    Thermo Fisher 7300plus real time pcr system
    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the <t>QuantStudio</t> Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)
    7300plus Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7300plus real time pcr system/product/Thermo Fisher
    Average 88 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    7300plus real time pcr system - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    90
    Thermo Fisher pikoreal real time pcr system
    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the <t>QuantStudio</t> Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)
    Pikoreal Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 924 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pikoreal real time pcr system/product/Thermo Fisher
    Average 90 stars, based on 924 article reviews
    Price from $9.99 to $1999.99
    pikoreal real time pcr system - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    99
    Thermo Fisher real time pcr qrt pcr
    RA induces ISX expression through RARs in CaCo-2 cells. CaCo-2 cells were seeded in DMEM and 10% FBS. After allowing the cells to adhere for 24 h, cells were treated with 1 μM RA or 1 μM 4-[( E )-2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl]benzoic acid (TTNPB) (a specific RAR agonist) or pretreated with 1 μM cycloheximide (CHX) and then 1 μM RA as indicated. After 4 h, total RNA was extracted and reverse transcribed, and <t>qRT-PCR</t> was performed with a specific probe set for ISX. mRNA levels were normalized for 18S rRNA expression. A ) ISX mRNA expression in CaCo-2 cells treated with either RA or TTNPB. B ) ISX mRNA levels in CaCo-2 cells pretreated with CHX and then with RA. Results are presented as fold induction vs . untreated control cells ( n =3/condition). C ) CaCo-2 cells treated with 1 μM RA or without (vehicle control) for 12 h were subjected to indirect immunostaining using the ISX primary antibody and Alexa Fluor-488 secondary antibody as indicated. ISX expression is detected as green fluorescence. Nuclei were also concurrently stained with DAPI, which was included in the mounting medium. Approximately 100 cells/experiment were counted; representative images from 3 independent experiments are shown. Images were acquired at ×40.
    Real Time Pcr Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2712 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr qrt pcr/product/Thermo Fisher
    Average 99 stars, based on 2712 article reviews
    Price from $9.99 to $1999.99
    real time pcr qrt pcr - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    95
    Stratagene real time pcr qrt pcr
    RA induces ISX expression through RARs in CaCo-2 cells. CaCo-2 cells were seeded in DMEM and 10% FBS. After allowing the cells to adhere for 24 h, cells were treated with 1 μM RA or 1 μM 4-[( E )-2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl]benzoic acid (TTNPB) (a specific RAR agonist) or pretreated with 1 μM cycloheximide (CHX) and then 1 μM RA as indicated. After 4 h, total RNA was extracted and reverse transcribed, and <t>qRT-PCR</t> was performed with a specific probe set for ISX. mRNA levels were normalized for 18S rRNA expression. A ) ISX mRNA expression in CaCo-2 cells treated with either RA or TTNPB. B ) ISX mRNA levels in CaCo-2 cells pretreated with CHX and then with RA. Results are presented as fold induction vs . untreated control cells ( n =3/condition). C ) CaCo-2 cells treated with 1 μM RA or without (vehicle control) for 12 h were subjected to indirect immunostaining using the ISX primary antibody and Alexa Fluor-488 secondary antibody as indicated. ISX expression is detected as green fluorescence. Nuclei were also concurrently stained with DAPI, which was included in the mounting medium. Approximately 100 cells/experiment were counted; representative images from 3 independent experiments are shown. Images were acquired at ×40.
    Real Time Pcr Qrt Pcr, supplied by Stratagene, used in various techniques. Bioz Stars score: 95/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr qrt pcr/product/Stratagene
    Average 95 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    real time pcr qrt pcr - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    90
    Thermo Fisher real time pcr machine
    RA induces ISX expression through RARs in CaCo-2 cells. CaCo-2 cells were seeded in DMEM and 10% FBS. After allowing the cells to adhere for 24 h, cells were treated with 1 μM RA or 1 μM 4-[( E )-2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl]benzoic acid (TTNPB) (a specific RAR agonist) or pretreated with 1 μM cycloheximide (CHX) and then 1 μM RA as indicated. After 4 h, total RNA was extracted and reverse transcribed, and <t>qRT-PCR</t> was performed with a specific probe set for ISX. mRNA levels were normalized for 18S rRNA expression. A ) ISX mRNA expression in CaCo-2 cells treated with either RA or TTNPB. B ) ISX mRNA levels in CaCo-2 cells pretreated with CHX and then with RA. Results are presented as fold induction vs . untreated control cells ( n =3/condition). C ) CaCo-2 cells treated with 1 μM RA or without (vehicle control) for 12 h were subjected to indirect immunostaining using the ISX primary antibody and Alexa Fluor-488 secondary antibody as indicated. ISX expression is detected as green fluorescence. Nuclei were also concurrently stained with DAPI, which was included in the mounting medium. Approximately 100 cells/experiment were counted; representative images from 3 independent experiments are shown. Images were acquired at ×40.
    Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1894 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr machine/product/Thermo Fisher
    Average 90 stars, based on 1894 article reviews
    Price from $9.99 to $1999.99
    real time pcr machine - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    87
    Promega real time pcr qrt pcr
    RA induces ISX expression through RARs in CaCo-2 cells. CaCo-2 cells were seeded in DMEM and 10% FBS. After allowing the cells to adhere for 24 h, cells were treated with 1 μM RA or 1 μM 4-[( E )-2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl]benzoic acid (TTNPB) (a specific RAR agonist) or pretreated with 1 μM cycloheximide (CHX) and then 1 μM RA as indicated. After 4 h, total RNA was extracted and reverse transcribed, and <t>qRT-PCR</t> was performed with a specific probe set for ISX. mRNA levels were normalized for 18S rRNA expression. A ) ISX mRNA expression in CaCo-2 cells treated with either RA or TTNPB. B ) ISX mRNA levels in CaCo-2 cells pretreated with CHX and then with RA. Results are presented as fold induction vs . untreated control cells ( n =3/condition). C ) CaCo-2 cells treated with 1 μM RA or without (vehicle control) for 12 h were subjected to indirect immunostaining using the ISX primary antibody and Alexa Fluor-488 secondary antibody as indicated. ISX expression is detected as green fluorescence. Nuclei were also concurrently stained with DAPI, which was included in the mounting medium. Approximately 100 cells/experiment were counted; representative images from 3 independent experiments are shown. Images were acquired at ×40.
    Real Time Pcr Qrt Pcr, supplied by Promega, used in various techniques. Bioz Stars score: 87/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr qrt pcr/product/Promega
    Average 87 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    real time pcr qrt pcr - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    97
    Roche real time pcr qrt pcr
    Reduction of HIF-1α levels induced by EZN-2968 oligonucleotide (A) MM1.S and U266 cell lines were incubated in the presence of either EZN-2968 or scrambled oligonucleotide (20 μmol/L) for up to 72h, in normoxic (pO 2 21%) or hypoxic (pO 2 1%) culture conditions. (i) HIF-1α mRNA expression (normalized to GAPDH level) and (ii) protein level (β-actin used as loading control) were then analyzed by <t>qRT-PCR</t> and Western Blotting, respectively. (iii) Immunoprecipitation assay was performed using anti-p300 antibody followed by immunoblotting assay using anti-HIF-1α antibody. MM1.S cells were treated with the HIF inhibitor for 48h prior to the assay. Representative data from one experiment are presented. (B) MM1.S cells were incubated in the presence of either EZN-2968 or scrambled oligonucleotide (20 μmol/L) up to 72h, and mRNA expression of HIF-2α was evaluated by qRT-PCR. (C) VEGF protein level secreted by MM1.S cells exposed to either EZN-2968 or scrambled oligonucleotide (20 μmol/L) were quantified by ELISA. Histograms show the mean value ± SD of three independent experiments. * p
    Real Time Pcr Qrt Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 680 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr qrt pcr/product/Roche
    Average 97 stars, based on 680 article reviews
    Price from $9.99 to $1999.99
    real time pcr qrt pcr - by Bioz Stars, 2020-02
    97/100 stars
      Buy from Supplier

    99
    TaKaRa real time pcr qrt pcr
    Effects of BANCR on the biological behaviors of Hep3B cells. a <t>qRT-PCR</t> analysis confirmed decreased BANCR expression in Hep3B cells after si-BANCR transfecting. BANCR expression was normalized to GAPDH. * P
    Real Time Pcr Qrt Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 505 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr qrt pcr/product/TaKaRa
    Average 99 stars, based on 505 article reviews
    Price from $9.99 to $1999.99
    real time pcr qrt pcr - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher flex real time pcr system
    CXCL12-Treated Cells Demonstrate Significant Up-Regulation of Transcripts Encoding COPII Vesicle Proteins. ( A ) Table of GO terms associated with specific genes encoding proteins that function in ER to Golgi transport that were significantly up-regulated by CXCL12, but not TGFβ, treatment. All of these genes encode proteins that comprise multi-subunit RING-finger type 3 Cullin-RBX E3 structures which, upon the addition of KLHL12, forms COPII vesicles essential for procollagen secretion. The gene Accession numbers and Symbols are as indicated. LogFC (log fold change) and FC (fold change) refer to CXCL12 > TGF β after normalization to vehicle; logCPM = log counts per million; FDR = false discovery rate. ( B , C ) Graphs demonstrating averages of triplicate measures of normalized (to vehicle) fold expression of CUL3 ( B ) and KLHL12 ( C ) in CXCL12- or TGF β -treated cells by <t>qRT-PCR</t> analysis confirm that both genes were more robustly transcriptionally induced by CXCL12 than TGF β , particularly at 8 hours treatment (* p
    Flex Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 795 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flex real time pcr system/product/Thermo Fisher
    Average 90 stars, based on 795 article reviews
    Price from $9.99 to $1999.99
    flex real time pcr system - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher quantstudio 6 flex qrt pcr system
    Validation of RNA-Seq results by <t>qRT-PCR.</t> Three biological replicates were performed. * indicates significant difference of milRNA/mRNA expression level in conidial vs. mycelial stages (*: P
    Quantstudio 6 Flex Qrt Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantstudio 6 flex qrt pcr system/product/Thermo Fisher
    Average 90 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    quantstudio 6 flex qrt pcr system - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher pikoreal 24 real time pcr machine
    Validation of RNA-Seq results by <t>qRT-PCR.</t> Three biological replicates were performed. * indicates significant difference of milRNA/mRNA expression level in conidial vs. mycelial stages (*: P
    Pikoreal 24 Real Time Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pikoreal 24 real time pcr machine/product/Thermo Fisher
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    pikoreal 24 real time pcr machine - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by qRT-PCR on a StepOnePlus real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P

    Journal: Oncotarget

    Article Title: An EBV recombinant deleted for residues 130-159 in EBNA3C can deregulate p53/Mdm2 and Cyclin D1/CDK6 which results in apoptosis and reduced cell proliferation

    doi: 10.18632/oncotarget.7502

    Figure Lengend Snippet: Analysis of mRNA and protein levels for pRb and E2F1 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of pRb and E2F1 were quantified by qRT-PCR on a StepOnePlus real-time PCR system. B. The protein levels of pRb and E2F1 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P

    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR System (Applied Biosystems Inc, Carlsbad, CA).

    Techniques: Infection, Synthesized, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot

    Analysis of mRNA and protein levels for p53, Mdm2, CyclinD1 and Cdk6 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of p53, Mdm2, CyclinD1 and Cdk6 were quantified by qRT-PCR on a StepOnePlus real-time PCR system. B. The protein levels of p53, Mdm2, CyclinD1 and Cdk6 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P

    Journal: Oncotarget

    Article Title: An EBV recombinant deleted for residues 130-159 in EBNA3C can deregulate p53/Mdm2 and Cyclin D1/CDK6 which results in apoptosis and reduced cell proliferation

    doi: 10.18632/oncotarget.7502

    Figure Lengend Snippet: Analysis of mRNA and protein levels for p53, Mdm2, CyclinD1 and Cdk6 during EBV primary infection at 2, 5, and 7 dpi A. Human PBMCs were infected by BACEBV-GFPWT (WT) and EBVGFPΔE3C130-159 (ΔE3C130-159) virus and cells were harvested at 2, 5, and 7 days p.i. Total RNAs were extracted by using TRIzol (Invitrogen), and cDNAs were synthesized using a high capacity RNA-to-cDNA kit. The mRNA levels of p53, Mdm2, CyclinD1 and Cdk6 were quantified by qRT-PCR on a StepOnePlus real-time PCR system. B. The protein levels of p53, Mdm2, CyclinD1 and Cdk6 in PBMC infected with BACEBV-GFPWT and EBVGFPΔE3C130-159 virus at 2, 5 and 7 days p.i. were analyzed Western blot. dpi, days post-infection; RQ, relative quantity; RI, relative intensity. *P

    Article Snippet: RT-qPCR was performed on a StepOnePlus real-time PCR System (Applied Biosystems Inc, Carlsbad, CA).

    Techniques: Infection, Synthesized, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Western Blot

    Effect of fucoxanthin rich powder on mRNA expression of transcription factor and enzymes related to antioxidant system in the liver of rats. Total RNA was isolated using TRI-reagenet, and cDNA was synthesized using 3 ug of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of the primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± S.E of three independent experiments. Different letters above each bar indicate significant differences among the groups at α = 0.05 as determined by Duncan's multiple range tests. HO-1: heme oxygenase (decycling) 1, Nrf2: nuclear factor, erythroid derived 2, like 2, NQO-1: NAD(P)H quinone oxidoreductase 1.

    Journal: Nutrition Research and Practice

    Article Title: Antioxidant effects of fucoxanthin rich powder in rats fed with high fat diet

    doi: 10.4162/nrp.2013.7.6.475

    Figure Lengend Snippet: Effect of fucoxanthin rich powder on mRNA expression of transcription factor and enzymes related to antioxidant system in the liver of rats. Total RNA was isolated using TRI-reagenet, and cDNA was synthesized using 3 ug of total RNA with SuperScript II reverse transcriptase. Realtime PCR was performed using SYBR green and standard procedures to assess the mRNA expression of the primer in liver samples obtained from each group. An Applied Biosystem StepOne softwere v2.1 was used. Each bar represents the mean ± S.E of three independent experiments. Different letters above each bar indicate significant differences among the groups at α = 0.05 as determined by Duncan's multiple range tests. HO-1: heme oxygenase (decycling) 1, Nrf2: nuclear factor, erythroid derived 2, like 2, NQO-1: NAD(P)H quinone oxidoreductase 1.

    Article Snippet: Ct-values of target genes and the reference gene were obtained using an Applied Biosystems StepOne Plus RT-PCR system (Applied biosystems, CA, USA).

    Techniques: Expressing, Isolation, Synthesized, Polymerase Chain Reaction, SYBR Green Assay, Derivative Assay

    Prox1 is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by PCR. Data represent one of three separate experiments.

    Journal: Oncotarget

    Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2

    doi: 10.18632/oncotarget.17278

    Figure Lengend Snippet: Prox1 is associated with IL-2 promoter Chromatin was extracted from ( A ) Jurkat cells and ( B ) naïve CD4 + T cells unstimulated or stimulated with anti-CD3/CD28 Dynabeads for 24 h, and precipitated with anti-Prox1 antibody or isotype IgG. The DNA sequence containing minimal IL-2 promoter (–256 to -46, product size: 211 bp) was analyzed by PCR. Data represent one of three separate experiments.

    Article Snippet: To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol.

    Techniques: Sequencing, Polymerase Chain Reaction

    Overexpression of Prox1 inhibits IL-2 expression ( A and B ) Lentiviral vector expressing Prox1 gene and GFP, or GFP alone, were generated. Jurkat cells were infected with Prox1 lentivirus or the control lentivirus. The GFP + Jurkat cells were sorted 48 h post-infection, and stimulated with anti-CD3/CD28 Dynabeads for indicated times. (A) Prox1 and (B) IL-2 mRNA levels were assessed by real-time PCR, while ( C ) IL-2 protein levels at 24 h were measured by ELISA. ( D ) Jurkat cells were transiently transfected with increasing amount of Prox1 plasmids (0.125, 0.25 and 0.5 μg/ml) or control vector (pcDNA3), and stimulated with anti-CD3/CD28 Dynabeads for 24 h. The IL-2 protein levels were measured by ELISA. The data from represent the mean ± SEM of three experiments, ** P

    Journal: Oncotarget

    Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2

    doi: 10.18632/oncotarget.17278

    Figure Lengend Snippet: Overexpression of Prox1 inhibits IL-2 expression ( A and B ) Lentiviral vector expressing Prox1 gene and GFP, or GFP alone, were generated. Jurkat cells were infected with Prox1 lentivirus or the control lentivirus. The GFP + Jurkat cells were sorted 48 h post-infection, and stimulated with anti-CD3/CD28 Dynabeads for indicated times. (A) Prox1 and (B) IL-2 mRNA levels were assessed by real-time PCR, while ( C ) IL-2 protein levels at 24 h were measured by ELISA. ( D ) Jurkat cells were transiently transfected with increasing amount of Prox1 plasmids (0.125, 0.25 and 0.5 μg/ml) or control vector (pcDNA3), and stimulated with anti-CD3/CD28 Dynabeads for 24 h. The IL-2 protein levels were measured by ELISA. The data from represent the mean ± SEM of three experiments, ** P

    Article Snippet: To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol.

    Techniques: Over Expression, Expressing, Plasmid Preparation, Generated, Infection, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Transfection

    Expression of Prox1 mRNA and protein in T cells ( A, B and C ) PBMCs, naïve CD4 + T cells and Jurkat cells were isolated, and stimulated with PHA and anti-CD3/CD28 Dynabeads for 24 h respectively. (A) Prox1 and (C) IL-2 mRNA levels were measured by RT-PCR, while (B) Prox1 protein levels were assessed by Western blot. For (A), (B) and (C), the data represent one of three independent experiments. ( D and E ) Naïve CD4 + T cells were stimulated with anti-CD3/CD28 Dynabeads for indicated times. (D) Prox1 and (E) IL-2 mRNA levels were measured by real-time PCR. Gene expression is normalized against the amount of GAPDH mRNA. The data from represent the mean ± SEM of three experiments.

    Journal: Oncotarget

    Article Title: Prox1 represses IL-2 gene expression by interacting with NFAT2

    doi: 10.18632/oncotarget.17278

    Figure Lengend Snippet: Expression of Prox1 mRNA and protein in T cells ( A, B and C ) PBMCs, naïve CD4 + T cells and Jurkat cells were isolated, and stimulated with PHA and anti-CD3/CD28 Dynabeads for 24 h respectively. (A) Prox1 and (C) IL-2 mRNA levels were measured by RT-PCR, while (B) Prox1 protein levels were assessed by Western blot. For (A), (B) and (C), the data represent one of three independent experiments. ( D and E ) Naïve CD4 + T cells were stimulated with anti-CD3/CD28 Dynabeads for indicated times. (D) Prox1 and (E) IL-2 mRNA levels were measured by real-time PCR. Gene expression is normalized against the amount of GAPDH mRNA. The data from represent the mean ± SEM of three experiments.

    Article Snippet: To check the mRNA levels of Prox1, IL-2 and GAPDH, real-time PCR was performed in triplicate with a real-time PCR system (ABI PRISM 7500; Applied Biosystems, Foster City, CA, USA) using a SYBR detection kit (Takara) according to the standard protocol.

    Techniques: Expressing, Isolation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Real-time Polymerase Chain Reaction

    The tissue RARRES2 gene expression level is down-regulated in ACC but is not associated with patient prognosis. A, TaqMan real-time quantitative PCR reveals down-regulation of the RARRES2 gene expression level in our cohort of ACC samples as compared

    Journal: The Journal of Clinical Endocrinology and Metabolism

    Article Title: Serum RARRES2 Is a Prognostic Marker in Patients With Adrenocortical Carcinoma

    doi: 10.1210/jc.2016-1781

    Figure Lengend Snippet: The tissue RARRES2 gene expression level is down-regulated in ACC but is not associated with patient prognosis. A, TaqMan real-time quantitative PCR reveals down-regulation of the RARRES2 gene expression level in our cohort of ACC samples as compared

    Article Snippet: TaqMan real-time quantitative polymerase chain reaction was performed on 7900HT fast real-time PCR systems (Applied Biosystems).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative PCR (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: The SUR2B subunit of rat vascular KATP channel is targeted by miR-9a-3p induced by prolonged exposure to methylglyoxal

    doi: 10.1152/ajpcell.00311.2014

    Figure Lengend Snippet: Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative PCR (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P

    Article Snippet: The qPCR was performed with a Fast Real-time PCR system (Applied Biosystems 7500) for 40 cycles.

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    Platform comparison. Allelic discrimination plots of the four GBA mutations. Water was used as the no template control. Fig. 1A: Validation on the ABI PRISM® 7900 HT Sequence Detection System. Fig. 1B: Validation on the Applied Biosystems ViiA™ 7 Real-Time PCR System. Fig. 1C: Blind validation on the ABI PRISM® 7900 HT Sequence Detection System.

    Journal: PLoS ONE

    Article Title: High-Throughput Carrier Screening Using TaqMan Allelic Discrimination

    doi: 10.1371/journal.pone.0059722

    Figure Lengend Snippet: Platform comparison. Allelic discrimination plots of the four GBA mutations. Water was used as the no template control. Fig. 1A: Validation on the ABI PRISM® 7900 HT Sequence Detection System. Fig. 1B: Validation on the Applied Biosystems ViiA™ 7 Real-Time PCR System. Fig. 1C: Blind validation on the ABI PRISM® 7900 HT Sequence Detection System.

    Article Snippet: The plates were centrifuged for 1 minute, sealed, and then run in duplex real time PCR reactions using FAM and VIC as the detector probes for each assay on both the ABI PRISM® 7900 HT Sequence Detection System (LTI) and the Applied Biosystems ViiA™ 7 Real-Time PCR System (LTI).

    Techniques: Sequencing, Real-time Polymerase Chain Reaction

    Real-time quantitative PCR analysis confirmed cercariae gender-associated DNA microarray results. PCR was performed using a MiniOpticon Real-Time PCR Detection System (Bio-Rad, UK) and SYBR Green I chemistry according to manufacturer's instructions. Briefly, real-time PCR parameters included 40 cycles, fluorescent reading after each cycle and melt curve analysis of individual products at the end of the 40 cycles. Unique oligonucleotide identification number is shown along with BLASTx annotation and gender association. Significant similarity is defined as (E) value≤10 −05 . Fold-difference was calculated as described in Methods . Primer characteristics (Tm, annealing temperature and sequence) along with amplicon size are included in the Dataset S1 (RT-PCR Primers). Underlined unique IDs represent gender-associated transcripts identified by the indirect hybridization strategy (parasite cDNA versus parasite gDNA). The italicized unique ID represents a gender-associated transcript identified by the direct hybridization strategy (female cDNA versus male cDNA). Plain text unique IDs represent gender-associated transcripts identified by both hybridization strategies.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Use of Genomic DNA as an Indirect Reference for Identifying Gender-Associated Transcripts in Morphologically Identical, but Chromosomally Distinct, Schistosoma mansoni Cercariae

    doi: 10.1371/journal.pntd.0000323

    Figure Lengend Snippet: Real-time quantitative PCR analysis confirmed cercariae gender-associated DNA microarray results. PCR was performed using a MiniOpticon Real-Time PCR Detection System (Bio-Rad, UK) and SYBR Green I chemistry according to manufacturer's instructions. Briefly, real-time PCR parameters included 40 cycles, fluorescent reading after each cycle and melt curve analysis of individual products at the end of the 40 cycles. Unique oligonucleotide identification number is shown along with BLASTx annotation and gender association. Significant similarity is defined as (E) value≤10 −05 . Fold-difference was calculated as described in Methods . Primer characteristics (Tm, annealing temperature and sequence) along with amplicon size are included in the Dataset S1 (RT-PCR Primers). Underlined unique IDs represent gender-associated transcripts identified by the indirect hybridization strategy (parasite cDNA versus parasite gDNA). The italicized unique ID represents a gender-associated transcript identified by the direct hybridization strategy (female cDNA versus male cDNA). Plain text unique IDs represent gender-associated transcripts identified by both hybridization strategies.

    Article Snippet: Real time PCR analysis Relative gene expression (fold difference between samples) was quantified relative to alpha-tubulin using a MiniOpticon Real-Time PCR Detection System (Bio-Rad) and SYBR green chemistry (Bio-Rad).

    Techniques: Real-time Polymerase Chain Reaction, Microarray, Polymerase Chain Reaction, SYBR Green Assay, Sequencing, Amplification, Reverse Transcription Polymerase Chain Reaction, Hybridization

    Relative expression of P. eryngii KNR2312 receptors (a) and pheromones (b) in the monokaryon (P6) and dikaryon (KNR2312), as determined by real-time qRT-PCR. Gene expression was normalized to 18S rRNA expression and calibrated to the value for the monokaryon (P6), which was assigned a value of 1, by the standard curve method (Bio-Rad). All assays were performed in triplicate. The error bars show the standard deviations for triplicate samples.

    Journal: PLoS ONE

    Article Title: Identification and Functional Analysis of Pheromone and Receptor Genes in the B3 Mating Locus of Pleurotus eryngii

    doi: 10.1371/journal.pone.0104693

    Figure Lengend Snippet: Relative expression of P. eryngii KNR2312 receptors (a) and pheromones (b) in the monokaryon (P6) and dikaryon (KNR2312), as determined by real-time qRT-PCR. Gene expression was normalized to 18S rRNA expression and calibrated to the value for the monokaryon (P6), which was assigned a value of 1, by the standard curve method (Bio-Rad). All assays were performed in triplicate. The error bars show the standard deviations for triplicate samples.

    Article Snippet: Analysis of gene expression by real-time qRT-PCR Real-time qRT-PCR (CFX96, Bio-Rad) was performed using KNR2312 (dikaryon) and P6 (monokaryon) to quantify the expression of the 4 receptor genes and the 4 pheromone genes.

    Techniques: Expressing, Quantitative RT-PCR

    RNA-seq analysis of melatonin-treated transcriptome. (A) Overlapping DEG numbers between M0/M10 andM0/M20. (B) Correlation of RNA-seq ( y -axis) and qRT-PCR date ( x -axis). The correlation assay was carried out for 36 DEGs with log 2 ratios > 1.0 or

    Journal: Frontiers in Plant Science

    Article Title: Melatonin Regulates Root Architecture by Modulating Auxin Response in Rice

    doi: 10.3389/fpls.2017.00134

    Figure Lengend Snippet: RNA-seq analysis of melatonin-treated transcriptome. (A) Overlapping DEG numbers between M0/M10 andM0/M20. (B) Correlation of RNA-seq ( y -axis) and qRT-PCR date ( x -axis). The correlation assay was carried out for 36 DEGs with log 2 ratios > 1.0 or

    Article Snippet: For quantitative real-time PCR (qRT-PCR), SYBR Green I was added to the reaction mix and run on a Chromo4 real-time PCR detection system according to the manufacturer's instructions (CFX96; Bio-Rad, California, USA).

    Techniques: RNA Sequencing Assay, Quantitative RT-PCR, Two-Photon Excitation Fluorescence Cross-Correlation Assay

    The relative expression pattern of auxin signaling pathway related genes. (A) The relative expression profile of co-up regulated auxin signaling genes in rice root in M0, M10, and M20, including five OsAux/IAA , four OsGH3 , one OsARF , and one OsSAUR genes. (B) Relative transcript levels of genes corresponding to (A) by qRT-PCR. M0, samples treated with water. M10, samples treated with 10 μM melatonin. M20, samples treated with 20 μM melatonin. Transcript levels are expressed relative to that of rice ACTIN1 in each sample, and values are mean SD ( n = 3). ** P ≤ 0.01. Student t -test was used to generate P -value.

    Journal: Frontiers in Plant Science

    Article Title: Melatonin Regulates Root Architecture by Modulating Auxin Response in Rice

    doi: 10.3389/fpls.2017.00134

    Figure Lengend Snippet: The relative expression pattern of auxin signaling pathway related genes. (A) The relative expression profile of co-up regulated auxin signaling genes in rice root in M0, M10, and M20, including five OsAux/IAA , four OsGH3 , one OsARF , and one OsSAUR genes. (B) Relative transcript levels of genes corresponding to (A) by qRT-PCR. M0, samples treated with water. M10, samples treated with 10 μM melatonin. M20, samples treated with 20 μM melatonin. Transcript levels are expressed relative to that of rice ACTIN1 in each sample, and values are mean SD ( n = 3). ** P ≤ 0.01. Student t -test was used to generate P -value.

    Article Snippet: For quantitative real-time PCR (qRT-PCR), SYBR Green I was added to the reaction mix and run on a Chromo4 real-time PCR detection system according to the manufacturer's instructions (CFX96; Bio-Rad, California, USA).

    Techniques: Expressing, Quantitative RT-PCR

    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the QuantStudio Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in serum and urine. a Normal human serum or b urine pooled from healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of each dilution were extracted using the small volume protocol (0.2 mL) and were tested with the Trioplex assay on the ABI7500 Fast Dx instrument. The same serum ( c ) and urine ( d ) dilutions were tested on the QuantStudio Dx instrument. Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: RNA Extraction, Standard Deviation

    Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between Trioplex assay multiplex and the ZIKV singleplex format assay using small volume and large volume RNA extraction. Normal human serum or urine was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD), and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicates of every dilution were extracted using the MagNA Pure 96 instrument (Roche) and tested by Trioplex assay multiplex or ZIKV singleplex format assay on the ABI 7500 Fast Dx or the QuantStudio Dx instruments. a Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum at each dilution on the ABI 7500 Fast Dx instrument. A linear regression was plotted for multiplex with small volume protocol (Sv) (0.2 mL) (black straight line), singleplex assay with small volume protocol (gray straight line), multiplex with large volume protocol (Lv) (1 mL) (black dashed line). and singleplex assay with large volume protocol (gray dashed line). b Compares the mean genome copy equivalents per PCR reaction (GCE/rxn) of viral RNA extracted from serum or urine at each dilution on the QuantStudio Dx instrument. A linear regression was plotted for multiplex with small volume protocol serum (Sv) (black straight line), multiplex with large volume protocol serum (Lv) (gray straight line), multiplex with small volume protocol urine (Lv) (black dashed line), and multiplex with large volume protocol urine (gray dashed line). Error bars represent GCE/mL standard deviation. The CT values for every dilution replicate in serum tested was plotted for c small volume and d large volume extractions

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: Multiplex Assay, RNA Extraction, Polymerase Chain Reaction, Singleplex Assay, Standard Deviation

    Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in whole blood (EDTA). A pool of whole blood donated by healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD) and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicas of every dilution were extracted using small volume protocol (0.2 mL) and tested with Trioplex assay on the ABI7500 Fast Dx instrument ( a ) or in the QuantStudio Dx instrument ( b ). Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Journal: Nature Communications

    Article Title: Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses

    doi: 10.1038/s41467-018-03772-1

    Figure Lengend Snippet: Analytical performance comparison between MP96 and LC 2.0 RNA extraction platforms in whole blood (EDTA). A pool of whole blood donated by healthy donors was contrived with ZIKV at a dilution of 1:10 before the limit of detection (1:10 BLoD), at the limit of detection (LoD) and at 1:10 after the limit of detection (1:10 ALoD). Twenty replicas of every dilution were extracted using small volume protocol (0.2 mL) and tested with Trioplex assay on the ABI7500 Fast Dx instrument ( a ) or in the QuantStudio Dx instrument ( b ). Mean genome copy equivalents per milliliter (GCE/mL) of viral RNA detected are displayed at each dilution. Error bars represent GCE/mL standard deviation. Straight line represents linear regression of MP96 platform (Roche). Dashed line represents linear regression of LC 2.0 platform (Roche)

    Article Snippet: The fluorophore-labeled hydrolysis probes bind to the amplified DNA target fragment and the intensity of the fluorescent signal is captured by a real-time PCR instrument: (ABI 7500 fast Dx (ThermoFisher) or QuantStudio Dx (ThermoFisher)).

    Techniques: RNA Extraction, Standard Deviation

    RA induces ISX expression through RARs in CaCo-2 cells. CaCo-2 cells were seeded in DMEM and 10% FBS. After allowing the cells to adhere for 24 h, cells were treated with 1 μM RA or 1 μM 4-[( E )-2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl]benzoic acid (TTNPB) (a specific RAR agonist) or pretreated with 1 μM cycloheximide (CHX) and then 1 μM RA as indicated. After 4 h, total RNA was extracted and reverse transcribed, and qRT-PCR was performed with a specific probe set for ISX. mRNA levels were normalized for 18S rRNA expression. A ) ISX mRNA expression in CaCo-2 cells treated with either RA or TTNPB. B ) ISX mRNA levels in CaCo-2 cells pretreated with CHX and then with RA. Results are presented as fold induction vs . untreated control cells ( n =3/condition). C ) CaCo-2 cells treated with 1 μM RA or without (vehicle control) for 12 h were subjected to indirect immunostaining using the ISX primary antibody and Alexa Fluor-488 secondary antibody as indicated. ISX expression is detected as green fluorescence. Nuclei were also concurrently stained with DAPI, which was included in the mounting medium. Approximately 100 cells/experiment were counted; representative images from 3 independent experiments are shown. Images were acquired at ×40.

    Journal: The FASEB Journal

    Article Title: ISX is a retinoic acid-sensitive gatekeeper that controls intestinal ?,?-carotene absorption and vitamin A production

    doi: 10.1096/fj.09-150995

    Figure Lengend Snippet: RA induces ISX expression through RARs in CaCo-2 cells. CaCo-2 cells were seeded in DMEM and 10% FBS. After allowing the cells to adhere for 24 h, cells were treated with 1 μM RA or 1 μM 4-[( E )-2-(5,6,7,8-tetrahydro-5,5,8,8-tetramethyl-2-naphthalenyl)-1-propenyl]benzoic acid (TTNPB) (a specific RAR agonist) or pretreated with 1 μM cycloheximide (CHX) and then 1 μM RA as indicated. After 4 h, total RNA was extracted and reverse transcribed, and qRT-PCR was performed with a specific probe set for ISX. mRNA levels were normalized for 18S rRNA expression. A ) ISX mRNA expression in CaCo-2 cells treated with either RA or TTNPB. B ) ISX mRNA levels in CaCo-2 cells pretreated with CHX and then with RA. Results are presented as fold induction vs . untreated control cells ( n =3/condition). C ) CaCo-2 cells treated with 1 μM RA or without (vehicle control) for 12 h were subjected to indirect immunostaining using the ISX primary antibody and Alexa Fluor-488 secondary antibody as indicated. ISX expression is detected as green fluorescence. Nuclei were also concurrently stained with DAPI, which was included in the mounting medium. Approximately 100 cells/experiment were counted; representative images from 3 independent experiments are shown. Images were acquired at ×40.

    Article Snippet: All reagents for quantitative real-time PCR (qRT-PCR) were purchased from Applied BioSystems (ABI; Foster City, CA, USA).

    Techniques: Expressing, Quantitative RT-PCR, Immunostaining, Fluorescence, Staining

    Reduction of HIF-1α levels induced by EZN-2968 oligonucleotide (A) MM1.S and U266 cell lines were incubated in the presence of either EZN-2968 or scrambled oligonucleotide (20 μmol/L) for up to 72h, in normoxic (pO 2 21%) or hypoxic (pO 2 1%) culture conditions. (i) HIF-1α mRNA expression (normalized to GAPDH level) and (ii) protein level (β-actin used as loading control) were then analyzed by qRT-PCR and Western Blotting, respectively. (iii) Immunoprecipitation assay was performed using anti-p300 antibody followed by immunoblotting assay using anti-HIF-1α antibody. MM1.S cells were treated with the HIF inhibitor for 48h prior to the assay. Representative data from one experiment are presented. (B) MM1.S cells were incubated in the presence of either EZN-2968 or scrambled oligonucleotide (20 μmol/L) up to 72h, and mRNA expression of HIF-2α was evaluated by qRT-PCR. (C) VEGF protein level secreted by MM1.S cells exposed to either EZN-2968 or scrambled oligonucleotide (20 μmol/L) were quantified by ELISA. Histograms show the mean value ± SD of three independent experiments. * p

    Journal: Oncotarget

    Article Title: Hypoxia inducible factor-1 alpha as a therapeutic target in multiple myeloma

    doi:

    Figure Lengend Snippet: Reduction of HIF-1α levels induced by EZN-2968 oligonucleotide (A) MM1.S and U266 cell lines were incubated in the presence of either EZN-2968 or scrambled oligonucleotide (20 μmol/L) for up to 72h, in normoxic (pO 2 21%) or hypoxic (pO 2 1%) culture conditions. (i) HIF-1α mRNA expression (normalized to GAPDH level) and (ii) protein level (β-actin used as loading control) were then analyzed by qRT-PCR and Western Blotting, respectively. (iii) Immunoprecipitation assay was performed using anti-p300 antibody followed by immunoblotting assay using anti-HIF-1α antibody. MM1.S cells were treated with the HIF inhibitor for 48h prior to the assay. Representative data from one experiment are presented. (B) MM1.S cells were incubated in the presence of either EZN-2968 or scrambled oligonucleotide (20 μmol/L) up to 72h, and mRNA expression of HIF-2α was evaluated by qRT-PCR. (C) VEGF protein level secreted by MM1.S cells exposed to either EZN-2968 or scrambled oligonucleotide (20 μmol/L) were quantified by ELISA. Histograms show the mean value ± SD of three independent experiments. * p

    Article Snippet: Real-time PCR (qRT-PCR) was performed by adding 2 μl of 20 μl cDNA to an universal master Mix (Lightcycler probe Master mix, Roche, Applied science), primers (0.5 μmol/L of each primer) and universal probes, UPL (0.2 μmol/L of each probe).

    Techniques: Incubation, Expressing, Quantitative RT-PCR, Western Blot, Immunoprecipitation, Enzyme-linked Immunosorbent Assay

    HIF-1α expression in multiple myeloma cells (A) Baseline level of HIF-1α mRNA (i) and protein product (ii) were assessed in four MM cell lines by qRT-PCR and Western Blotting (50 μg/lane) analysis, respectively. (B) HIF-1α protein expression in CD138 + cells derived from bone marrow aspirates from MM patients was monitored using immunofluorescence microscopy. The percentage of HIF-1α positive cells was calculated evaluating at least 200 cells. Green fluorescence: HIF-1α; Blue fluorescence: DAPI. (C) To evaluate the pro-survival stimuli modulation of HIF-1α mRNA expression (i) and protein synthesis (ii), the MM1.S cells were treated with IGF-1 (100 ng/ml) or IL-6 (50 ng/ml) for 4h. The effect of 24h treatment with CoCl 2 (100 μM) on HIF-1α stabilization was taken as control. The immunoblot assays were performed twice with similar results. Representative data from one experiment are presented. mRNA expression of HIF-1α was normalized to GAPDH level. Histograms show the mean value ± SD of three independent experiments. ** p

    Journal: Oncotarget

    Article Title: Hypoxia inducible factor-1 alpha as a therapeutic target in multiple myeloma

    doi:

    Figure Lengend Snippet: HIF-1α expression in multiple myeloma cells (A) Baseline level of HIF-1α mRNA (i) and protein product (ii) were assessed in four MM cell lines by qRT-PCR and Western Blotting (50 μg/lane) analysis, respectively. (B) HIF-1α protein expression in CD138 + cells derived from bone marrow aspirates from MM patients was monitored using immunofluorescence microscopy. The percentage of HIF-1α positive cells was calculated evaluating at least 200 cells. Green fluorescence: HIF-1α; Blue fluorescence: DAPI. (C) To evaluate the pro-survival stimuli modulation of HIF-1α mRNA expression (i) and protein synthesis (ii), the MM1.S cells were treated with IGF-1 (100 ng/ml) or IL-6 (50 ng/ml) for 4h. The effect of 24h treatment with CoCl 2 (100 μM) on HIF-1α stabilization was taken as control. The immunoblot assays were performed twice with similar results. Representative data from one experiment are presented. mRNA expression of HIF-1α was normalized to GAPDH level. Histograms show the mean value ± SD of three independent experiments. ** p

    Article Snippet: Real-time PCR (qRT-PCR) was performed by adding 2 μl of 20 μl cDNA to an universal master Mix (Lightcycler probe Master mix, Roche, Applied science), primers (0.5 μmol/L of each primer) and universal probes, UPL (0.2 μmol/L of each probe).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Derivative Assay, Immunofluorescence, Microscopy, Fluorescence

    Effects of BANCR on the biological behaviors of Hep3B cells. a qRT-PCR analysis confirmed decreased BANCR expression in Hep3B cells after si-BANCR transfecting. BANCR expression was normalized to GAPDH. * P

    Journal: World Journal of Surgical Oncology

    Article Title: Increased expression of LncRNA BANCR and its prognostic significance in human hepatocellular carcinoma

    doi: 10.1186/s12957-015-0757-5

    Figure Lengend Snippet: Effects of BANCR on the biological behaviors of Hep3B cells. a qRT-PCR analysis confirmed decreased BANCR expression in Hep3B cells after si-BANCR transfecting. BANCR expression was normalized to GAPDH. * P

    Article Snippet: BANCR expression levels were measured with quantitative real-time PCR (qRT-PCR) using an ABI7500 system and the SYBR Green PCR Master Mix (Takara).

    Techniques: Quantitative RT-PCR, Expressing

    CXCL12-Treated Cells Demonstrate Significant Up-Regulation of Transcripts Encoding COPII Vesicle Proteins. ( A ) Table of GO terms associated with specific genes encoding proteins that function in ER to Golgi transport that were significantly up-regulated by CXCL12, but not TGFβ, treatment. All of these genes encode proteins that comprise multi-subunit RING-finger type 3 Cullin-RBX E3 structures which, upon the addition of KLHL12, forms COPII vesicles essential for procollagen secretion. The gene Accession numbers and Symbols are as indicated. LogFC (log fold change) and FC (fold change) refer to CXCL12 > TGF β after normalization to vehicle; logCPM = log counts per million; FDR = false discovery rate. ( B , C ) Graphs demonstrating averages of triplicate measures of normalized (to vehicle) fold expression of CUL3 ( B ) and KLHL12 ( C ) in CXCL12- or TGF β -treated cells by qRT-PCR analysis confirm that both genes were more robustly transcriptionally induced by CXCL12 than TGF β , particularly at 8 hours treatment (* p

    Journal: Scientific Reports

    Article Title: CXCL12/CXCR4-Mediated Procollagen Secretion Is Coupled To Cullin-RING Ubiquitin Ligase Activation

    doi: 10.1038/s41598-018-21506-7

    Figure Lengend Snippet: CXCL12-Treated Cells Demonstrate Significant Up-Regulation of Transcripts Encoding COPII Vesicle Proteins. ( A ) Table of GO terms associated with specific genes encoding proteins that function in ER to Golgi transport that were significantly up-regulated by CXCL12, but not TGFβ, treatment. All of these genes encode proteins that comprise multi-subunit RING-finger type 3 Cullin-RBX E3 structures which, upon the addition of KLHL12, forms COPII vesicles essential for procollagen secretion. The gene Accession numbers and Symbols are as indicated. LogFC (log fold change) and FC (fold change) refer to CXCL12 > TGF β after normalization to vehicle; logCPM = log counts per million; FDR = false discovery rate. ( B , C ) Graphs demonstrating averages of triplicate measures of normalized (to vehicle) fold expression of CUL3 ( B ) and KLHL12 ( C ) in CXCL12- or TGF β -treated cells by qRT-PCR analysis confirm that both genes were more robustly transcriptionally induced by CXCL12 than TGF β , particularly at 8 hours treatment (* p

    Article Snippet: Following quantification, 1 ug of RNA was reverse transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Carlsbad, CA). qRT-PCR was performed using a QuantStudio 12 K Flex Real-Time PCR System, reagents and software (Applied Biosystems, Carlsbad, CA).

    Techniques: Expressing, Quantitative RT-PCR

    Validation of RNA-Seq results by qRT-PCR. Three biological replicates were performed. * indicates significant difference of milRNA/mRNA expression level in conidial vs. mycelial stages (*: P

    Journal: BMC Genomics

    Article Title: Integrated microRNA and mRNA analysis in the pathogenic filamentous fungus Trichophyton rubrum

    doi: 10.1186/s12864-018-5316-3

    Figure Lengend Snippet: Validation of RNA-Seq results by qRT-PCR. Three biological replicates were performed. * indicates significant difference of milRNA/mRNA expression level in conidial vs. mycelial stages (*: P

    Article Snippet: In order to validate the expression changes of miRNAs and their target genes between conidia and mycelia in T. rubrum , the relative expression levels of 9 randomly selected milRNAs and 20 target genes were analyzed by qRT-PCR on a QuantStudio™ 6 Flex qRT-PCR system (Applied Biosystems, Foster City, CA, USA).

    Techniques: RNA Sequencing Assay, Quantitative RT-PCR, Expressing