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  • 99
    Thermo Fisher real time quantitative reverse transcription polymerase chain reaction qrt pcr
    Real Time Quantitative Reverse Transcription Polymerase Chain Reaction Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche real time quantitative reverse transcription polymerase chain reaction qrt pcr
    Senescence state of human adipose-derived mesenchymal stem cells (hASCs) in different age-groups. (A) Representative pictures of senescence-associated β-galactosidase (SA-β-gal) staining (20× magnification). (B) The number of senescent cells (SA-β-gal-positive) was significantly higher in the elderly group. Positive cells were expressed as a percentage of total hASCs counted. (C) Total intracellular ROS production was measured using the DCFH-DA assay. (D) Quantitative analysis of the DCFH-DA assay revealed a significant increase in ROS production in the elderly group. (E) Representative pictures of MitoSOX Red staining demonstrating that mitochondrial-specific ROS production was increased in the elderly group. (F) Proteasome activity assay showed a decreasing trend with age increasing, although the difference was not significant. (G) Messenger RNA (mRNA) expression levels of SIRT1 and hTERT were determined by <t>qRT-PCR.</t> Although there was no difference in their expression between the different age-groups, both exhibit a decreasing trend with increasing age. (H) qRT-PCR analysis showed that the mRNA expression levels of senescence markers p53, p21 , and p16 were increased with increasing donor age. The mRNA levels of RB1 declined in an age-dependent manner, whereas the RB2 expression level in 3 groups was comparable. Only p21 mRNA expression showed significant difference between the child group and the elderly group. The expression of each gene was normalized to the amount of GAPDH RNA to calculate the relative amount of mRNA. All tests were performed in triplicate, and the data are expressed as the mean ± standard error of mean. * P
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    Bio-Rad real time quantitative reverse transcription polymerase chain reaction qrt pcr system
    Senescence state of human adipose-derived mesenchymal stem cells (hASCs) in different age-groups. (A) Representative pictures of senescence-associated β-galactosidase (SA-β-gal) staining (20× magnification). (B) The number of senescent cells (SA-β-gal-positive) was significantly higher in the elderly group. Positive cells were expressed as a percentage of total hASCs counted. (C) Total intracellular ROS production was measured using the DCFH-DA assay. (D) Quantitative analysis of the DCFH-DA assay revealed a significant increase in ROS production in the elderly group. (E) Representative pictures of MitoSOX Red staining demonstrating that mitochondrial-specific ROS production was increased in the elderly group. (F) Proteasome activity assay showed a decreasing trend with age increasing, although the difference was not significant. (G) Messenger RNA (mRNA) expression levels of SIRT1 and hTERT were determined by <t>qRT-PCR.</t> Although there was no difference in their expression between the different age-groups, both exhibit a decreasing trend with increasing age. (H) qRT-PCR analysis showed that the mRNA expression levels of senescence markers p53, p21 , and p16 were increased with increasing donor age. The mRNA levels of RB1 declined in an age-dependent manner, whereas the RB2 expression level in 3 groups was comparable. Only p21 mRNA expression showed significant difference between the child group and the elderly group. The expression of each gene was normalized to the amount of GAPDH RNA to calculate the relative amount of mRNA. All tests were performed in triplicate, and the data are expressed as the mean ± standard error of mean. * P
    Real Time Quantitative Reverse Transcription Polymerase Chain Reaction Qrt Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa real time quantitative reverse transcription polymerase chain reaction qrt pcr rnaiso plus
    Senescence state of human adipose-derived mesenchymal stem cells (hASCs) in different age-groups. (A) Representative pictures of senescence-associated β-galactosidase (SA-β-gal) staining (20× magnification). (B) The number of senescent cells (SA-β-gal-positive) was significantly higher in the elderly group. Positive cells were expressed as a percentage of total hASCs counted. (C) Total intracellular ROS production was measured using the DCFH-DA assay. (D) Quantitative analysis of the DCFH-DA assay revealed a significant increase in ROS production in the elderly group. (E) Representative pictures of MitoSOX Red staining demonstrating that mitochondrial-specific ROS production was increased in the elderly group. (F) Proteasome activity assay showed a decreasing trend with age increasing, although the difference was not significant. (G) Messenger RNA (mRNA) expression levels of SIRT1 and hTERT were determined by <t>qRT-PCR.</t> Although there was no difference in their expression between the different age-groups, both exhibit a decreasing trend with increasing age. (H) qRT-PCR analysis showed that the mRNA expression levels of senescence markers p53, p21 , and p16 were increased with increasing donor age. The mRNA levels of RB1 declined in an age-dependent manner, whereas the RB2 expression level in 3 groups was comparable. Only p21 mRNA expression showed significant difference between the child group and the elderly group. The expression of each gene was normalized to the amount of GAPDH RNA to calculate the relative amount of mRNA. All tests were performed in triplicate, and the data are expressed as the mean ± standard error of mean. * P
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    Thermo Fisher quantitative real time reverse transcription polymerase chain reaction
    Osteogenic differentiation. The numbers in each plot indicate the different hUCMSC seeding densities on CPC-microbead-fiber scaffold, where 1k=1000. <t>Real-time</t> <t>reverse</t> <t>transcription</t> <t>polymerase</t> <t>chain</t> <t>reaction</t> on gene expression for (A) alkaline phosphatase
    Quantitative Real Time Reverse Transcription Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Osteogenic differentiation. The numbers in each plot indicate the different hUCMSC seeding densities on CPC-microbead-fiber scaffold, where 1k=1000. <t>Real-time</t> <t>reverse</t> <t>transcription</t> <t>polymerase</t> <t>chain</t> <t>reaction</t> on gene expression for (A) alkaline phosphatase
    Real Time Quantitative Reverse Transcription Polymerase Chain Reactions Rt Pcrs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time quantitative reverse transcript polymerase chain reaction qrt pcr
    ASCs express the machinery for production and secretion of ACh. (A) Real‐time <t>qRT‐PCR</t> comparison of the expression of Chat and Slc18a3 . Compared with uASCs, the dASCs had a significantly higher expression of Chat and Slc18a3 . Slc18a3, vesicular acetylcholine transporter. (B) Immunocytochemistry of uASCs and dASCs using antibody against choline acetyltransferase (ChAT; red). DAPI was used to stain the nuclei (blue). * P
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    ASCs express the machinery for production and secretion of ACh. (A) Real‐time <t>qRT‐PCR</t> comparison of the expression of Chat and Slc18a3 . Compared with uASCs, the dASCs had a significantly higher expression of Chat and Slc18a3 . Slc18a3, vesicular acetylcholine transporter. (B) Immunocytochemistry of uASCs and dASCs using antibody against choline acetyltransferase (ChAT; red). DAPI was used to stain the nuclei (blue). * P
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    ASCs express the machinery for production and secretion of ACh. (A) Real‐time <t>qRT‐PCR</t> comparison of the expression of Chat and Slc18a3 . Compared with uASCs, the dASCs had a significantly higher expression of Chat and Slc18a3 . Slc18a3, vesicular acetylcholine transporter. (B) Immunocytochemistry of uASCs and dASCs using antibody against choline acetyltransferase (ChAT; red). DAPI was used to stain the nuclei (blue). * P
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    Promega quantitative real time polymerase chain reaction qrt pcr improm ii reverse transcription system
    ASCs express the machinery for production and secretion of ACh. (A) Real‐time <t>qRT‐PCR</t> comparison of the expression of Chat and Slc18a3 . Compared with uASCs, the dASCs had a significantly higher expression of Chat and Slc18a3 . Slc18a3, vesicular acetylcholine transporter. (B) Immunocytochemistry of uASCs and dASCs using antibody against choline acetyltransferase (ChAT; red). DAPI was used to stain the nuclei (blue). * P
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    Thermo Fisher one step real time quantitative reverse transcription polymerase chain reaction rt pcr kit
    ASCs express the machinery for production and secretion of ACh. (A) Real‐time <t>qRT‐PCR</t> comparison of the expression of Chat and Slc18a3 . Compared with uASCs, the dASCs had a significantly higher expression of Chat and Slc18a3 . Slc18a3, vesicular acetylcholine transporter. (B) Immunocytochemistry of uASCs and dASCs using antibody against choline acetyltransferase (ChAT; red). DAPI was used to stain the nuclei (blue). * P
    One Step Real Time Quantitative Reverse Transcription Polymerase Chain Reaction Rt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Genecopoeia mirna quantitative real time polymerase chain reaction quantitative reverse transcription polymerase chain reaction pcr
    ASCs express the machinery for production and secretion of ACh. (A) Real‐time <t>qRT‐PCR</t> comparison of the expression of Chat and Slc18a3 . Compared with uASCs, the dASCs had a significantly higher expression of Chat and Slc18a3 . Slc18a3, vesicular acetylcholine transporter. (B) Immunocytochemistry of uASCs and dASCs using antibody against choline acetyltransferase (ChAT; red). DAPI was used to stain the nuclei (blue). * P
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    <t>qRT-PCR</t> analysis of TaBiP transcriptional expression in different wheat organs, developing seeds, and under drought stress. (a) Expression in wheat organs. (b) Expression in developing seeds and under drought stress. Yanyou 361 CK (Y361CK) was not treated, and Yanyou 361 GH (Y361GH) was drought-treated.
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    Effects of pioglitazone treatment on SCD-1 and LXRα mRNA expression. RAW264.7 cells were pretreated with pioglitazone (10 μmol/L) for 6 hours and stimulated with or without palmitate (400 μmol/L) for 16 hours. Expression of SCD-1 (A), LXRα, Abca1 (C) and Abcg1 (D) mRNA was determined by <t>qRT-PCR.</t> Expression of SCD-1, LXRα, Abca1, and Abcg1 mRNA was presented as the relative level to that of 18S rRNA. **p
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    Bio-Rad real time quantitative reverse transcription polymerase chain reaction qrt pcr
    <t>qRT-PCR</t> confirmation of snoRNA expression. SNORD46 and SNORD89 were confirmed to be down–regulated in tumor, relative to normal samples using qRT-PCR platform. The Ct values obtained for snoRNAs were normalized to Ct values obtained for RNU6. * indicates statistical significance p
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    <t>qRT-PCR</t> confirmation of snoRNA expression. SNORD46 and SNORD89 were confirmed to be down–regulated in tumor, relative to normal samples using qRT-PCR platform. The Ct values obtained for snoRNAs were normalized to Ct values obtained for RNU6. * indicates statistical significance p
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    <t>qRT-PCR</t> confirmation of snoRNA expression. SNORD46 and SNORD89 were confirmed to be down–regulated in tumor, relative to normal samples using qRT-PCR platform. The Ct values obtained for snoRNAs were normalized to Ct values obtained for RNU6. * indicates statistical significance p
    Quantitative Real Time Reverse Transcription Polymerase Chain Reaction Pcr, supplied by Toyobo, used in various techniques. Bioz Stars score: 89/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    <t>qRT-PCR</t> confirmation of snoRNA expression. SNORD46 and SNORD89 were confirmed to be down–regulated in tumor, relative to normal samples using qRT-PCR platform. The Ct values obtained for snoRNAs were normalized to Ct values obtained for RNU6. * indicates statistical significance p
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    MiR-29c is an independent prognostic factor for glioma patient overcome A. miR-29a, miR-29b and miR-29c expression were downregulated in human glioma tissues examined by using <t>qRT-PCR.</t> Tumor: glioma tissues; Normal: the matched normal brain tissues. B. Kaplan-Meier survival curves for OS in relation to miR-29a, miR-29b and miR-29c expression. Cutoff values for miR-29s (high/low expression) were determined by ROC analysis by using SPSS16.0 software.
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    Image Search Results


    Senescence state of human adipose-derived mesenchymal stem cells (hASCs) in different age-groups. (A) Representative pictures of senescence-associated β-galactosidase (SA-β-gal) staining (20× magnification). (B) The number of senescent cells (SA-β-gal-positive) was significantly higher in the elderly group. Positive cells were expressed as a percentage of total hASCs counted. (C) Total intracellular ROS production was measured using the DCFH-DA assay. (D) Quantitative analysis of the DCFH-DA assay revealed a significant increase in ROS production in the elderly group. (E) Representative pictures of MitoSOX Red staining demonstrating that mitochondrial-specific ROS production was increased in the elderly group. (F) Proteasome activity assay showed a decreasing trend with age increasing, although the difference was not significant. (G) Messenger RNA (mRNA) expression levels of SIRT1 and hTERT were determined by qRT-PCR. Although there was no difference in their expression between the different age-groups, both exhibit a decreasing trend with increasing age. (H) qRT-PCR analysis showed that the mRNA expression levels of senescence markers p53, p21 , and p16 were increased with increasing donor age. The mRNA levels of RB1 declined in an age-dependent manner, whereas the RB2 expression level in 3 groups was comparable. Only p21 mRNA expression showed significant difference between the child group and the elderly group. The expression of each gene was normalized to the amount of GAPDH RNA to calculate the relative amount of mRNA. All tests were performed in triplicate, and the data are expressed as the mean ± standard error of mean. * P

    Journal: Cell Transplantation

    Article Title: Adipose-Derived Mesenchymal Stem Cells from the Elderly Exhibit Decreased Migration and Differentiation Abilities with Senescent Properties

    doi: 10.1177/0963689717721221

    Figure Lengend Snippet: Senescence state of human adipose-derived mesenchymal stem cells (hASCs) in different age-groups. (A) Representative pictures of senescence-associated β-galactosidase (SA-β-gal) staining (20× magnification). (B) The number of senescent cells (SA-β-gal-positive) was significantly higher in the elderly group. Positive cells were expressed as a percentage of total hASCs counted. (C) Total intracellular ROS production was measured using the DCFH-DA assay. (D) Quantitative analysis of the DCFH-DA assay revealed a significant increase in ROS production in the elderly group. (E) Representative pictures of MitoSOX Red staining demonstrating that mitochondrial-specific ROS production was increased in the elderly group. (F) Proteasome activity assay showed a decreasing trend with age increasing, although the difference was not significant. (G) Messenger RNA (mRNA) expression levels of SIRT1 and hTERT were determined by qRT-PCR. Although there was no difference in their expression between the different age-groups, both exhibit a decreasing trend with increasing age. (H) qRT-PCR analysis showed that the mRNA expression levels of senescence markers p53, p21 , and p16 were increased with increasing donor age. The mRNA levels of RB1 declined in an age-dependent manner, whereas the RB2 expression level in 3 groups was comparable. Only p21 mRNA expression showed significant difference between the child group and the elderly group. The expression of each gene was normalized to the amount of GAPDH RNA to calculate the relative amount of mRNA. All tests were performed in triplicate, and the data are expressed as the mean ± standard error of mean. * P

    Article Snippet: The expression levels of c-Jun, c-Fos, caspase8, Bcl-2 , and CHEK1 genes were detected by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Roche, LightCycler 480, Switzerland).

    Techniques: Derivative Assay, Staining, DCFH-DA Assay, Activity Assay, Expressing, Quantitative RT-PCR

    Effect of donor age on human adipose-derived mesenchymal stem cells (hASCs) colony-forming unit fibroblasts (CFU-Fs), proliferation, apoptosis, and cell cycle distribution. (A) CFU-Fs assay showed the number of clone forming cells decreased significantly in elderly group compared with the child group. (B) Donor age had no effect on hASCs proliferation, as evidenced by the MTS assay. (C) qRT-PCR analysis of genes associated with proliferation ( c-Jun and c-Fos ) showing a decreasing trend with age. (D) Apoptosis was analyzed using a Muse Cell Analyzer, showing no significant difference between the different age-groups. (E) Expression of apoptosis-related genes Bcl-2 and caspase-8 were determined by qRT-PCR and did not appear to be significantly affected by donor age. (F) Cell cycle distribution of hASCs in different age-groups analyzed with a Muse Cell Analyzer. The number of cells in S phase in the elderly group was significantly decreased compared with the other 2 groups. (G) qRT-PCR analysis showed that the expression of CHEK1 , a cell cycle-associated gene, was upregulated with increasing age, although the difference was not significant. Experiments were carried out in triplicate. Data are expressed as the mean ± standard error of mean [SEM]. *P

    Journal: Cell Transplantation

    Article Title: Adipose-Derived Mesenchymal Stem Cells from the Elderly Exhibit Decreased Migration and Differentiation Abilities with Senescent Properties

    doi: 10.1177/0963689717721221

    Figure Lengend Snippet: Effect of donor age on human adipose-derived mesenchymal stem cells (hASCs) colony-forming unit fibroblasts (CFU-Fs), proliferation, apoptosis, and cell cycle distribution. (A) CFU-Fs assay showed the number of clone forming cells decreased significantly in elderly group compared with the child group. (B) Donor age had no effect on hASCs proliferation, as evidenced by the MTS assay. (C) qRT-PCR analysis of genes associated with proliferation ( c-Jun and c-Fos ) showing a decreasing trend with age. (D) Apoptosis was analyzed using a Muse Cell Analyzer, showing no significant difference between the different age-groups. (E) Expression of apoptosis-related genes Bcl-2 and caspase-8 were determined by qRT-PCR and did not appear to be significantly affected by donor age. (F) Cell cycle distribution of hASCs in different age-groups analyzed with a Muse Cell Analyzer. The number of cells in S phase in the elderly group was significantly decreased compared with the other 2 groups. (G) qRT-PCR analysis showed that the expression of CHEK1 , a cell cycle-associated gene, was upregulated with increasing age, although the difference was not significant. Experiments were carried out in triplicate. Data are expressed as the mean ± standard error of mean [SEM]. *P

    Article Snippet: The expression levels of c-Jun, c-Fos, caspase8, Bcl-2 , and CHEK1 genes were detected by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Roche, LightCycler 480, Switzerland).

    Techniques: Derivative Assay, MTS Assay, Quantitative RT-PCR, Expressing

    Osteogenic differentiation diminishes with increasing age. (A) Representative pictures of alkaline phosphatase (ALP) staining (20× magnification). (B, C) Messenger RNA (mRNA) expression levels of osteogenic differentiation-related genes ( ALP, RUNX2, BMP2, OCN , and OPN ) were determined in human adipose-derived mesenchymal stem cells (hASCs) over the course of osteogenic differentiation by qRT-PCR. hASCs from aged donors had a diminished response to osteogenic inducers relative to that of young donors. (D) Alizarin Red S staining was used to visualize mineralization. Representative images were shown from 3 separate experiments. (E) Quantitative analysis of Alizarin Red S staining in hASCs cultures at day 14 after osteogenic induction revealed a significant decrease in matrix calcification in the elderly group compared to younger donors. All tests were performed in triplicate, and the data are expressed as the mean ± standard error of mean (SEM). * P

    Journal: Cell Transplantation

    Article Title: Adipose-Derived Mesenchymal Stem Cells from the Elderly Exhibit Decreased Migration and Differentiation Abilities with Senescent Properties

    doi: 10.1177/0963689717721221

    Figure Lengend Snippet: Osteogenic differentiation diminishes with increasing age. (A) Representative pictures of alkaline phosphatase (ALP) staining (20× magnification). (B, C) Messenger RNA (mRNA) expression levels of osteogenic differentiation-related genes ( ALP, RUNX2, BMP2, OCN , and OPN ) were determined in human adipose-derived mesenchymal stem cells (hASCs) over the course of osteogenic differentiation by qRT-PCR. hASCs from aged donors had a diminished response to osteogenic inducers relative to that of young donors. (D) Alizarin Red S staining was used to visualize mineralization. Representative images were shown from 3 separate experiments. (E) Quantitative analysis of Alizarin Red S staining in hASCs cultures at day 14 after osteogenic induction revealed a significant decrease in matrix calcification in the elderly group compared to younger donors. All tests were performed in triplicate, and the data are expressed as the mean ± standard error of mean (SEM). * P

    Article Snippet: The expression levels of c-Jun, c-Fos, caspase8, Bcl-2 , and CHEK1 genes were detected by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Roche, LightCycler 480, Switzerland).

    Techniques: ALP Assay, Staining, Expressing, Derivative Assay, Quantitative RT-PCR

    Aging influences adipogenic differentiation potential at the early time of induction, while the overall potential to form mature adipocytes is comparable between age-groups. (A) Adipogenesis was confirmed in hASCs by Oil Red O staining. (B) Semi-quantitative detection by colorimetric evaluation of Oil Red O uptake showed no significant differences between the 3 groups. (C, D) Adipogenic differentiation was further confirmed through qRT-PCR analysis of genes related to adipogenic differentiation ( PPAR -γ , CEBP-α, FABP4 , adiponectin, and LPL ). At the early time of induction (day 4), all genes related to adipogenic differentiation were significantly higher in the child group compared to the other 2 groups. (E) The expression of lipid synthesis-related genes ( GPDH, FASN, ACC1 , and HSL ) in hASCs after adipogenic induction for 4, 8, and 12 d. There were no significant differences between the different age-groups. (F) The expression levels of thermogenic markers UCP1 and PGC1α were significantly higher in the child group at the early induction stage. All tests were performed in triplicate, and the data are expressed as the mean ± standard error of mean (SEM). * P

    Journal: Cell Transplantation

    Article Title: Adipose-Derived Mesenchymal Stem Cells from the Elderly Exhibit Decreased Migration and Differentiation Abilities with Senescent Properties

    doi: 10.1177/0963689717721221

    Figure Lengend Snippet: Aging influences adipogenic differentiation potential at the early time of induction, while the overall potential to form mature adipocytes is comparable between age-groups. (A) Adipogenesis was confirmed in hASCs by Oil Red O staining. (B) Semi-quantitative detection by colorimetric evaluation of Oil Red O uptake showed no significant differences between the 3 groups. (C, D) Adipogenic differentiation was further confirmed through qRT-PCR analysis of genes related to adipogenic differentiation ( PPAR -γ , CEBP-α, FABP4 , adiponectin, and LPL ). At the early time of induction (day 4), all genes related to adipogenic differentiation were significantly higher in the child group compared to the other 2 groups. (E) The expression of lipid synthesis-related genes ( GPDH, FASN, ACC1 , and HSL ) in hASCs after adipogenic induction for 4, 8, and 12 d. There were no significant differences between the different age-groups. (F) The expression levels of thermogenic markers UCP1 and PGC1α were significantly higher in the child group at the early induction stage. All tests were performed in triplicate, and the data are expressed as the mean ± standard error of mean (SEM). * P

    Article Snippet: The expression levels of c-Jun, c-Fos, caspase8, Bcl-2 , and CHEK1 genes were detected by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Roche, LightCycler 480, Switzerland).

    Techniques: Staining, Quantitative RT-PCR, Expressing

    Osteogenic differentiation. The numbers in each plot indicate the different hUCMSC seeding densities on CPC-microbead-fiber scaffold, where 1k=1000. Real-time reverse transcription polymerase chain reaction on gene expression for (A) alkaline phosphatase

    Journal: Tissue Engineering. Part A

    Article Title: Effect of Cell Seeding Density on Proliferation and Osteodifferentiation of Umbilical Cord Stem Cells on Calcium Phosphate Cement-Fiber Scaffold

    doi: 10.1089/ten.tea.2011.0048

    Figure Lengend Snippet: Osteogenic differentiation. The numbers in each plot indicate the different hUCMSC seeding densities on CPC-microbead-fiber scaffold, where 1k=1000. Real-time reverse transcription polymerase chain reaction on gene expression for (A) alkaline phosphatase

    Article Snippet: Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR, 7900HT; Applied Biosystems) was used. hUCMSCs attaching to CPC-microbead-fiber scaffold were cultured for 1, 4, 8, and 14 days in osteogenic media.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

    Quantitative analysis of expression of microRNA(miR)‐92a and other endogenous miRs as controls (miR‐123, miR‐203, and miR‐126) in ischemic myocardial tissue compared to nonischemic control myocardium of 2 replicate samples obtained at 1, 3, and 10 days after induction of ischemia (49 minutes) and intracoronary injection of encapsulated antagomir‐92a. Myocardial tissue was snap‐frozen and stored at −80°C for RNA analysis. Expression of miRs is represented as difference in cycles of amplification (ΔCt) in control versus ischemic zone assessed by real‐time RT‐PCR reaction. d indicates day. Data are mean±SEM. n=3. RT‐PCR indicates reverse transcription polymerase chain reaction.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Single Intracoronary Injection of Encapsulated Antagomir‐92a Promotes Angiogenesis and Prevents Adverse Infarct Remodeling

    doi: 10.1161/JAHA.114.000946

    Figure Lengend Snippet: Quantitative analysis of expression of microRNA(miR)‐92a and other endogenous miRs as controls (miR‐123, miR‐203, and miR‐126) in ischemic myocardial tissue compared to nonischemic control myocardium of 2 replicate samples obtained at 1, 3, and 10 days after induction of ischemia (49 minutes) and intracoronary injection of encapsulated antagomir‐92a. Myocardial tissue was snap‐frozen and stored at −80°C for RNA analysis. Expression of miRs is represented as difference in cycles of amplification (ΔCt) in control versus ischemic zone assessed by real‐time RT‐PCR reaction. d indicates day. Data are mean±SEM. n=3. RT‐PCR indicates reverse transcription polymerase chain reaction.

    Article Snippet: For analysis of microRNA expression in heart samples, we carried out a SYBR® Green–based real‐time quantitative reverse transcription polymerase chain reaction (Applied Biosystems) with specific primer sets for amplification (miR‐92a, miR‐123, miR‐203, and miR‐126 [Exiqon]).

    Techniques: Expressing, Injection, RNA Expression, Amplification, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction

    ASCs express the machinery for production and secretion of ACh. (A) Real‐time qRT‐PCR comparison of the expression of Chat and Slc18a3 . Compared with uASCs, the dASCs had a significantly higher expression of Chat and Slc18a3 . Slc18a3, vesicular acetylcholine transporter. (B) Immunocytochemistry of uASCs and dASCs using antibody against choline acetyltransferase (ChAT; red). DAPI was used to stain the nuclei (blue). * P

    Journal: Muscle & Nerve

    Article Title: Adipose stem cells enhance myoblast proliferation via acetylcholine and extracellular signal–regulated kinase 1/2 signaling

    doi: 10.1002/mus.25741

    Figure Lengend Snippet: ASCs express the machinery for production and secretion of ACh. (A) Real‐time qRT‐PCR comparison of the expression of Chat and Slc18a3 . Compared with uASCs, the dASCs had a significantly higher expression of Chat and Slc18a3 . Slc18a3, vesicular acetylcholine transporter. (B) Immunocytochemistry of uASCs and dASCs using antibody against choline acetyltransferase (ChAT; red). DAPI was used to stain the nuclei (blue). * P

    Article Snippet: Real‐time quantitative reverse transcript polymerase chain reaction (qRT‐PCR) was performed with a gene expression assay (TaqMan; Applied Biosystems) and real‐time PCR system (ViiA 7; Applied Biosystems).

    Techniques: Quantitative RT-PCR, Expressing, Immunocytochemistry, Staining

    qRT-PCR analysis of TaBiP transcriptional expression in different wheat organs, developing seeds, and under drought stress. (a) Expression in wheat organs. (b) Expression in developing seeds and under drought stress. Yanyou 361 CK (Y361CK) was not treated, and Yanyou 361 GH (Y361GH) was drought-treated.

    Journal: BMC Plant Biology

    Article Title: Molecular cloning, phylogenetic analysis, and expression profiling of endoplasmic reticulum molecular chaperone BiP genes from bread wheat (Triticum aestivum L.)

    doi: 10.1186/s12870-014-0260-0

    Figure Lengend Snippet: qRT-PCR analysis of TaBiP transcriptional expression in different wheat organs, developing seeds, and under drought stress. (a) Expression in wheat organs. (b) Expression in developing seeds and under drought stress. Yanyou 361 CK (Y361CK) was not treated, and Yanyou 361 GH (Y361GH) was drought-treated.

    Article Snippet: Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) The PrimeScript™ RT reagent Kit with gDNA Eraser provided by TaKaRa was used for RNA purification and reverse transcription following the manufacturer’s instructions.

    Techniques: Quantitative RT-PCR, Expressing

    HMW-GS compositions and dynamic transcriptional expression profiles of three TaBiP genes in a set of NILs as revealed by qRT-PCR. (a) HMW-GS compositions in the NILs by SDS-PAGE. (b–d) Expression of TaBiP1, - 2, and -3 in developing NILs wheat seeds.

    Journal: BMC Plant Biology

    Article Title: Molecular cloning, phylogenetic analysis, and expression profiling of endoplasmic reticulum molecular chaperone BiP genes from bread wheat (Triticum aestivum L.)

    doi: 10.1186/s12870-014-0260-0

    Figure Lengend Snippet: HMW-GS compositions and dynamic transcriptional expression profiles of three TaBiP genes in a set of NILs as revealed by qRT-PCR. (a) HMW-GS compositions in the NILs by SDS-PAGE. (b–d) Expression of TaBiP1, - 2, and -3 in developing NILs wheat seeds.

    Article Snippet: Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) The PrimeScript™ RT reagent Kit with gDNA Eraser provided by TaKaRa was used for RNA purification and reverse transcription following the manufacturer’s instructions.

    Techniques: Expressing, Quantitative RT-PCR, SDS Page

    Effects of pioglitazone treatment on SCD-1 and LXRα mRNA expression. RAW264.7 cells were pretreated with pioglitazone (10 μmol/L) for 6 hours and stimulated with or without palmitate (400 μmol/L) for 16 hours. Expression of SCD-1 (A), LXRα, Abca1 (C) and Abcg1 (D) mRNA was determined by qRT-PCR. Expression of SCD-1, LXRα, Abca1, and Abcg1 mRNA was presented as the relative level to that of 18S rRNA. **p

    Journal: PLoS ONE

    Article Title: PPARγ Agonists Attenuate Palmitate-Induced ER Stress through Up-Regulation of SCD-1 in Macrophages

    doi: 10.1371/journal.pone.0128546

    Figure Lengend Snippet: Effects of pioglitazone treatment on SCD-1 and LXRα mRNA expression. RAW264.7 cells were pretreated with pioglitazone (10 μmol/L) for 6 hours and stimulated with or without palmitate (400 μmol/L) for 16 hours. Expression of SCD-1 (A), LXRα, Abca1 (C) and Abcg1 (D) mRNA was determined by qRT-PCR. Expression of SCD-1, LXRα, Abca1, and Abcg1 mRNA was presented as the relative level to that of 18S rRNA. **p

    Article Snippet: Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was performed using THUNDERBIRD SYBR qPCR mix (TOYOBO) and the ABI PRISM 7500 Sequence Detection System (Applied Biosystems, Foster City, CA, USA).

    Techniques: Expressing, Quantitative RT-PCR

    qRT-PCR confirmation of snoRNA expression. SNORD46 and SNORD89 were confirmed to be down–regulated in tumor, relative to normal samples using qRT-PCR platform. The Ct values obtained for snoRNAs were normalized to Ct values obtained for RNU6. * indicates statistical significance p

    Journal: PLoS ONE

    Article Title: Profiling of Small Nucleolar RNAs by Next Generation Sequencing: Potential New Players for Breast Cancer Prognosis

    doi: 10.1371/journal.pone.0162622

    Figure Lengend Snippet: qRT-PCR confirmation of snoRNA expression. SNORD46 and SNORD89 were confirmed to be down–regulated in tumor, relative to normal samples using qRT-PCR platform. The Ct values obtained for snoRNAs were normalized to Ct values obtained for RNU6. * indicates statistical significance p

    Article Snippet: Real time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using an iScript Select cDNA Synthesis Kit (Bio-Rad) and a SsoFast EvaGreen Supermix (Bio-Rad) according to manufacturers’ instructions.

    Techniques: Quantitative RT-PCR, Expressing

    MiR-29c is an independent prognostic factor for glioma patient overcome A. miR-29a, miR-29b and miR-29c expression were downregulated in human glioma tissues examined by using qRT-PCR. Tumor: glioma tissues; Normal: the matched normal brain tissues. B. Kaplan-Meier survival curves for OS in relation to miR-29a, miR-29b and miR-29c expression. Cutoff values for miR-29s (high/low expression) were determined by ROC analysis by using SPSS16.0 software.

    Journal: Oncotarget

    Article Title: miR-29c contribute to glioma cells temozolomide sensitivity by targeting O6-methylguanine-DNA methyltransferases indirectly

    doi: 10.18632/oncotarget.10357

    Figure Lengend Snippet: MiR-29c is an independent prognostic factor for glioma patient overcome A. miR-29a, miR-29b and miR-29c expression were downregulated in human glioma tissues examined by using qRT-PCR. Tumor: glioma tissues; Normal: the matched normal brain tissues. B. Kaplan-Meier survival curves for OS in relation to miR-29a, miR-29b and miR-29c expression. Cutoff values for miR-29s (high/low expression) were determined by ROC analysis by using SPSS16.0 software.

    Article Snippet: Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) was performed using the All-in-One™ miRNA qRT-PCR detection kit (GeneCopoeia, Rockville, MD, USA) for miR-29 and small nuclear RNA U6, which was used as an endogenous control.

    Techniques: Expressing, Quantitative RT-PCR, Software