rdna New England Biolabs Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs 16s rrna gene
    16s Rrna Gene, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 526 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene/product/New England Biolabs
    Average 99 stars, based on 526 article reviews
    Price from $9.99 to $1999.99
    16s rrna gene - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs ribosomal rna depletion
    Regulation of mRNA and ecRNA by neuronal activity. ( a ) <t>PolyA+</t> <t>RNA-seq</t> following 1 h neuronal depolarization (25 mM KCl) or inactivation (1 μM TTX) reveals altered mRNA expression at a small subset of genes. Top, heatmap of KCl-altered transcripts (each column=1 biological replicate; 2 replicates per treatment). Bottom, Venn diagram of overlap between transcripts altered by KCl and TTX. ( b ) Corresponding heatmaps from PolyA− RNA-seq reveal relationship between activity-related mRNA and ecRNA changes. PolyA− RNA transcription from 5′, intronic and 3′ sites all correlated significantly with mRNA changes following neuronal depolarization with KCl (linear regression, P
    Ribosomal Rna Depletion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribosomal rna depletion/product/New England Biolabs
    Average 99 stars, based on 123 article reviews
    Price from $9.99 to $1999.99
    ribosomal rna depletion - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs 5 gcttgacgggcggtgt 3
    Regulation of mRNA and ecRNA by neuronal activity. ( a ) <t>PolyA+</t> <t>RNA-seq</t> following 1 h neuronal depolarization (25 mM KCl) or inactivation (1 μM TTX) reveals altered mRNA expression at a small subset of genes. Top, heatmap of KCl-altered transcripts (each column=1 biological replicate; 2 replicates per treatment). Bottom, Venn diagram of overlap between transcripts altered by KCl and TTX. ( b ) Corresponding heatmaps from PolyA− RNA-seq reveal relationship between activity-related mRNA and ecRNA changes. PolyA− RNA transcription from 5′, intronic and 3′ sites all correlated significantly with mRNA changes following neuronal depolarization with KCl (linear regression, P
    5 Gcttgacgggcggtgt 3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 gcttgacgggcggtgt 3/product/New England Biolabs
    Average 99 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    5 gcttgacgggcggtgt 3 - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs hin diii
    PFGE migration of undigested and I- Ceu I-digested genomic <t>DNAs</t> of O. intermedium strains ADV1 and ADV3. (A) Migration of high-molecular-weight fragments. Lanes 1 and 2, undigested DNA from strain ADV1 (lane 1) and strain ADV3 (lane 2); lanes 3 and 4, I- Ceu I-digested DNA from strain ADV1 (lane 3) and strain ADV3 (lane 4); Lanes Sc and Sp , Saccharomyces cerevisiae ( Sc ) and Schizosaccharomyces pombe ( Sp ) chromosomes (Bio-Rad) as molecular size markers. (B) Migration of low-molecular-weight I- Ceu I fragments. Lane 1, strain ADV1; lane 2, strain ADV3. Chromosomes and I- Ceu I digestion patterns of O. intermedium strains ADV2 and ADV4 to -7 are identical to patterns for strains ADV1 and ADV3, respectively (data not shown). A mixture of λ digested by Hin <t>dIII,</t> the λ concatemer, and Saccharomyces cerevisiae chromosomes was used as the molecular size marker (lane λ/ Sc ); the bands useful for the measure were, from the bottom, 27, 50, 100, 150, 200, 225, 250, and 285 kb.
    Hin Diii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hin diii/product/New England Biolabs
    Average 99 stars, based on 2304 article reviews
    Price from $9.99 to $1999.99
    hin diii - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs 16s rrna gene pcr amplicon barcoding
    A heatmap of bacterial genera in soil, spread and iChip retrieved microbial communities based on <t>16S</t> <t>rRNA</t> <t>amplicon</t> sequencing. Columns with similar annotations were collapsed by calculating the mean for each group. Rows depict identified operational taxonomic units (OTUs) with a summed relative abundance > 0.1%. Row names represent the lowest taxonomic rank for a given OTU: g—genus, f—family, o—order, c—class. Rows were centered by subtracting the row means (omitting NAs) of OTUs from their corresponding row; scaling was performed by dividing the (centered) row of OTUs by their standard deviations. The relative abundance of an OTU to which unit variance scaling was applied, in soil, spread and iChip recovered microbial communities ranges from −2 to 2 as shown in the lower heatmap key. Rows were clustered using Euclidean distance and average linkage. Columns were clustered using correlation distance and average linkage. The heatmap was constructed using R pheatmap package (Metsalu Vilo, 2015 ).
    16s Rrna Gene Pcr Amplicon Barcoding, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene pcr amplicon barcoding/product/New England Biolabs
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    16s rrna gene pcr amplicon barcoding - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    90
    New England Biolabs rna escherichia coli rrna
    A heatmap of bacterial genera in soil, spread and iChip retrieved microbial communities based on <t>16S</t> <t>rRNA</t> <t>amplicon</t> sequencing. Columns with similar annotations were collapsed by calculating the mean for each group. Rows depict identified operational taxonomic units (OTUs) with a summed relative abundance > 0.1%. Row names represent the lowest taxonomic rank for a given OTU: g—genus, f—family, o—order, c—class. Rows were centered by subtracting the row means (omitting NAs) of OTUs from their corresponding row; scaling was performed by dividing the (centered) row of OTUs by their standard deviations. The relative abundance of an OTU to which unit variance scaling was applied, in soil, spread and iChip recovered microbial communities ranges from −2 to 2 as shown in the lower heatmap key. Rows were clustered using Euclidean distance and average linkage. Columns were clustered using correlation distance and average linkage. The heatmap was constructed using R pheatmap package (Metsalu Vilo, 2015 ).
    Rna Escherichia Coli Rrna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna escherichia coli rrna/product/New England Biolabs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rna escherichia coli rrna - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    99
    New England Biolabs next ultra dna library prep kit
    A heatmap of bacterial genera in soil, spread and iChip retrieved microbial communities based on <t>16S</t> <t>rRNA</t> <t>amplicon</t> sequencing. Columns with similar annotations were collapsed by calculating the mean for each group. Rows depict identified operational taxonomic units (OTUs) with a summed relative abundance > 0.1%. Row names represent the lowest taxonomic rank for a given OTU: g—genus, f—family, o—order, c—class. Rows were centered by subtracting the row means (omitting NAs) of OTUs from their corresponding row; scaling was performed by dividing the (centered) row of OTUs by their standard deviations. The relative abundance of an OTU to which unit variance scaling was applied, in soil, spread and iChip recovered microbial communities ranges from −2 to 2 as shown in the lower heatmap key. Rows were clustered using Euclidean distance and average linkage. Columns were clustered using correlation distance and average linkage. The heatmap was constructed using R pheatmap package (Metsalu Vilo, 2015 ).
    Next Ultra Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 594 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/next ultra dna library prep kit/product/New England Biolabs
    Average 99 stars, based on 594 article reviews
    Price from $9.99 to $1999.99
    next ultra dna library prep kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Regulation of mRNA and ecRNA by neuronal activity. ( a ) PolyA+ RNA-seq following 1 h neuronal depolarization (25 mM KCl) or inactivation (1 μM TTX) reveals altered mRNA expression at a small subset of genes. Top, heatmap of KCl-altered transcripts (each column=1 biological replicate; 2 replicates per treatment). Bottom, Venn diagram of overlap between transcripts altered by KCl and TTX. ( b ) Corresponding heatmaps from PolyA− RNA-seq reveal relationship between activity-related mRNA and ecRNA changes. PolyA− RNA transcription from 5′, intronic and 3′ sites all correlated significantly with mRNA changes following neuronal depolarization with KCl (linear regression, P

    Journal: Nature Communications

    Article Title: Extra-coding RNAs regulate neuronal DNA methylation dynamics

    doi: 10.1038/ncomms12091

    Figure Lengend Snippet: Regulation of mRNA and ecRNA by neuronal activity. ( a ) PolyA+ RNA-seq following 1 h neuronal depolarization (25 mM KCl) or inactivation (1 μM TTX) reveals altered mRNA expression at a small subset of genes. Top, heatmap of KCl-altered transcripts (each column=1 biological replicate; 2 replicates per treatment). Bottom, Venn diagram of overlap between transcripts altered by KCl and TTX. ( b ) Corresponding heatmaps from PolyA− RNA-seq reveal relationship between activity-related mRNA and ecRNA changes. PolyA− RNA transcription from 5′, intronic and 3′ sites all correlated significantly with mRNA changes following neuronal depolarization with KCl (linear regression, P

    Article Snippet: The remaining non-polyadenylated (PolyA−) underwent ribosomal RNA depletion (NEBNext rRNA depletion kit).

    Techniques: Activity Assay, RNA Sequencing Assay, Expressing

    Genome-wide identification and quantification of ecRNAs from neuronal systems. ( a ) RNA-seq workflow identifies both polyadenylated and non-polyadenylated transcripts from the same neuronal tissue. ( b ) Comparison of PolyA+ and PolyA− sequencing from representative gene loci reveals PolyA− transcripts arising from intronic and post-TESs. ( c ) Genome wide, extra-coding transcripts were characterized by averaging PolyA− reads that mapped to 5′ (pre-TSS), intronic or 3′ (post-TES) of a given gene. ( d ) Rank plot of ecRNA index at 17,719 rat genes. ( e ) mRNA expression (PolyA+ RNA-seq) ranked by ecRNA index reveals correlation between ecRNA and mRNA expression. ( f ) Division of ecRNA into discrete quartiles reveals general profile and expression of PolyA− RNA transcripts. Data are aligned to transcription start sites (TSS) and TESs. Heatmap shows PolyA− transcription from all genes. ( g ) MBD-seq reveals metagenomic DNA methylation profiles, including hypomethylation at TSS and hypermethylation at TES. ecRNA transcription is associated with hypomethylated promoters across the genome. ( h , i ) Genome wide, ecRNA levels are positively correlated with mRNA transcription (( h ) one-way ANOVA, F (3,17715) =612.5, P

    Journal: Nature Communications

    Article Title: Extra-coding RNAs regulate neuronal DNA methylation dynamics

    doi: 10.1038/ncomms12091

    Figure Lengend Snippet: Genome-wide identification and quantification of ecRNAs from neuronal systems. ( a ) RNA-seq workflow identifies both polyadenylated and non-polyadenylated transcripts from the same neuronal tissue. ( b ) Comparison of PolyA+ and PolyA− sequencing from representative gene loci reveals PolyA− transcripts arising from intronic and post-TESs. ( c ) Genome wide, extra-coding transcripts were characterized by averaging PolyA− reads that mapped to 5′ (pre-TSS), intronic or 3′ (post-TES) of a given gene. ( d ) Rank plot of ecRNA index at 17,719 rat genes. ( e ) mRNA expression (PolyA+ RNA-seq) ranked by ecRNA index reveals correlation between ecRNA and mRNA expression. ( f ) Division of ecRNA into discrete quartiles reveals general profile and expression of PolyA− RNA transcripts. Data are aligned to transcription start sites (TSS) and TESs. Heatmap shows PolyA− transcription from all genes. ( g ) MBD-seq reveals metagenomic DNA methylation profiles, including hypomethylation at TSS and hypermethylation at TES. ecRNA transcription is associated with hypomethylated promoters across the genome. ( h , i ) Genome wide, ecRNA levels are positively correlated with mRNA transcription (( h ) one-way ANOVA, F (3,17715) =612.5, P

    Article Snippet: The remaining non-polyadenylated (PolyA−) underwent ribosomal RNA depletion (NEBNext rRNA depletion kit).

    Techniques: Genome Wide, RNA Sequencing Assay, Sequencing, Expressing, DNA Methylation Assay

    PFGE migration of undigested and I- Ceu I-digested genomic DNAs of O. intermedium strains ADV1 and ADV3. (A) Migration of high-molecular-weight fragments. Lanes 1 and 2, undigested DNA from strain ADV1 (lane 1) and strain ADV3 (lane 2); lanes 3 and 4, I- Ceu I-digested DNA from strain ADV1 (lane 3) and strain ADV3 (lane 4); Lanes Sc and Sp , Saccharomyces cerevisiae ( Sc ) and Schizosaccharomyces pombe ( Sp ) chromosomes (Bio-Rad) as molecular size markers. (B) Migration of low-molecular-weight I- Ceu I fragments. Lane 1, strain ADV1; lane 2, strain ADV3. Chromosomes and I- Ceu I digestion patterns of O. intermedium strains ADV2 and ADV4 to -7 are identical to patterns for strains ADV1 and ADV3, respectively (data not shown). A mixture of λ digested by Hin dIII, the λ concatemer, and Saccharomyces cerevisiae chromosomes was used as the molecular size marker (lane λ/ Sc ); the bands useful for the measure were, from the bottom, 27, 50, 100, 150, 200, 225, 250, and 285 kb.

    Journal: Journal of Bacteriology

    Article Title: Atypical 16S rRNA Gene Copies in Ochrobactrum intermedium Strains Reveal a Large Genomic Rearrangement by Recombination between rrn Copies

    doi: 10.1128/JB.185.9.2901-2909.2003

    Figure Lengend Snippet: PFGE migration of undigested and I- Ceu I-digested genomic DNAs of O. intermedium strains ADV1 and ADV3. (A) Migration of high-molecular-weight fragments. Lanes 1 and 2, undigested DNA from strain ADV1 (lane 1) and strain ADV3 (lane 2); lanes 3 and 4, I- Ceu I-digested DNA from strain ADV1 (lane 3) and strain ADV3 (lane 4); Lanes Sc and Sp , Saccharomyces cerevisiae ( Sc ) and Schizosaccharomyces pombe ( Sp ) chromosomes (Bio-Rad) as molecular size markers. (B) Migration of low-molecular-weight I- Ceu I fragments. Lane 1, strain ADV1; lane 2, strain ADV3. Chromosomes and I- Ceu I digestion patterns of O. intermedium strains ADV2 and ADV4 to -7 are identical to patterns for strains ADV1 and ADV3, respectively (data not shown). A mixture of λ digested by Hin dIII, the λ concatemer, and Saccharomyces cerevisiae chromosomes was used as the molecular size marker (lane λ/ Sc ); the bands useful for the measure were, from the bottom, 27, 50, 100, 150, 200, 225, 250, and 285 kb.

    Article Snippet: The copy number of 16S rRNA gene was determined by a Southern blotting experiment, using a 16S rDNA probe, on genomic DNAs digested by Hin dIII (restriction site absent from the 16S rDNA in the genus Ochrobactrum ).

    Techniques: Migration, Molecular Weight, Marker

    Copy numbers of 16S rDNA (A) and 46-bp insertion (B) in the genome of O. intermedium. Shown are Southern blots of Hin dIII-digested genomic DNA from strains PR17/sat (lane 1), ADV1 (lane 2), ADV3 (lane 3), and ADV9 (lane 4) and reference strain LMG 3301 T (lane 5) hybridized with the 16S rDNA probe (A) and the 46-bp insertion probe (B). Strain ADV2 showed a hybridization profile identical to that of strain ADV1; strains ADV4 to -7 showed hybridization profiles identical to that of strain ADV3; strains ADV10, ADV11, ADV14, and ADV24 showed hybridization profiles identical to the strain LMG 3301 T profile (data not shown). Sizes of hybridizing fragments were calculated by using λ digested by Hin dIII as a molecular marker.

    Journal: Journal of Bacteriology

    Article Title: Atypical 16S rRNA Gene Copies in Ochrobactrum intermedium Strains Reveal a Large Genomic Rearrangement by Recombination between rrn Copies

    doi: 10.1128/JB.185.9.2901-2909.2003

    Figure Lengend Snippet: Copy numbers of 16S rDNA (A) and 46-bp insertion (B) in the genome of O. intermedium. Shown are Southern blots of Hin dIII-digested genomic DNA from strains PR17/sat (lane 1), ADV1 (lane 2), ADV3 (lane 3), and ADV9 (lane 4) and reference strain LMG 3301 T (lane 5) hybridized with the 16S rDNA probe (A) and the 46-bp insertion probe (B). Strain ADV2 showed a hybridization profile identical to that of strain ADV1; strains ADV4 to -7 showed hybridization profiles identical to that of strain ADV3; strains ADV10, ADV11, ADV14, and ADV24 showed hybridization profiles identical to the strain LMG 3301 T profile (data not shown). Sizes of hybridizing fragments were calculated by using λ digested by Hin dIII as a molecular marker.

    Article Snippet: The copy number of 16S rRNA gene was determined by a Southern blotting experiment, using a 16S rDNA probe, on genomic DNAs digested by Hin dIII (restriction site absent from the 16S rDNA in the genus Ochrobactrum ).

    Techniques: Hybridization, Marker

    A heatmap of bacterial genera in soil, spread and iChip retrieved microbial communities based on 16S rRNA amplicon sequencing. Columns with similar annotations were collapsed by calculating the mean for each group. Rows depict identified operational taxonomic units (OTUs) with a summed relative abundance > 0.1%. Row names represent the lowest taxonomic rank for a given OTU: g—genus, f—family, o—order, c—class. Rows were centered by subtracting the row means (omitting NAs) of OTUs from their corresponding row; scaling was performed by dividing the (centered) row of OTUs by their standard deviations. The relative abundance of an OTU to which unit variance scaling was applied, in soil, spread and iChip recovered microbial communities ranges from −2 to 2 as shown in the lower heatmap key. Rows were clustered using Euclidean distance and average linkage. Columns were clustered using correlation distance and average linkage. The heatmap was constructed using R pheatmap package (Metsalu Vilo, 2015 ).

    Journal: Ecology and Evolution

    Article Title: A practical introduction to microbial molecular ecology through the use of isolation chips. A practical introduction to microbial molecular ecology through the use of isolation chips

    doi: 10.1002/ece3.4748

    Figure Lengend Snippet: A heatmap of bacterial genera in soil, spread and iChip retrieved microbial communities based on 16S rRNA amplicon sequencing. Columns with similar annotations were collapsed by calculating the mean for each group. Rows depict identified operational taxonomic units (OTUs) with a summed relative abundance > 0.1%. Row names represent the lowest taxonomic rank for a given OTU: g—genus, f—family, o—order, c—class. Rows were centered by subtracting the row means (omitting NAs) of OTUs from their corresponding row; scaling was performed by dividing the (centered) row of OTUs by their standard deviations. The relative abundance of an OTU to which unit variance scaling was applied, in soil, spread and iChip recovered microbial communities ranges from −2 to 2 as shown in the lower heatmap key. Rows were clustered using Euclidean distance and average linkage. Columns were clustered using correlation distance and average linkage. The heatmap was constructed using R pheatmap package (Metsalu Vilo, 2015 ).

    Article Snippet: 2.7.2 Library preparation Illumina libraries were prepared using a Nextera XT kit, following the manufacturer's recommendations for 16S rRNA gene PCR amplicon barcoding (using 2× NEBNext High‐Fidelity PCR master mix), clean up and pooling.

    Techniques: Amplification, Sequencing, Construct

    Taxonomic phylum distribution of bacterial communities in soil samples and colonies recovered using spread and iChip isolation techniques from Hagg Farm (HF) and Three Hagges Wood Meadow (THW) sites using 16S rRNA gene amplicon sequencing. The spread plate sample marked with * showed more similarity to the iChip samples based on phylum distribution and PCoA (Figure 2 c). The soil sample marked with # appears to have been sequenced twice

    Journal: Ecology and Evolution

    Article Title: A practical introduction to microbial molecular ecology through the use of isolation chips. A practical introduction to microbial molecular ecology through the use of isolation chips

    doi: 10.1002/ece3.4748

    Figure Lengend Snippet: Taxonomic phylum distribution of bacterial communities in soil samples and colonies recovered using spread and iChip isolation techniques from Hagg Farm (HF) and Three Hagges Wood Meadow (THW) sites using 16S rRNA gene amplicon sequencing. The spread plate sample marked with * showed more similarity to the iChip samples based on phylum distribution and PCoA (Figure 2 c). The soil sample marked with # appears to have been sequenced twice

    Article Snippet: 2.7.2 Library preparation Illumina libraries were prepared using a Nextera XT kit, following the manufacturer's recommendations for 16S rRNA gene PCR amplicon barcoding (using 2× NEBNext High‐Fidelity PCR master mix), clean up and pooling.

    Techniques: Isolation, Amplification, Sequencing

    Experimental design of molecular microbial ecology group project. The 18‐week project was divided into three parts: (1) field work, (2) lab work, (3) data analysis and reporting. During fieldwork, students were provided with iChips that they loaded with soil dilutions (1a) and then buried in dedicated locations for 2 weeks (1b). Initial lab work included preparation of soil extract agar plates, plating soil dilutions, and incubated iChip wells (2a) and overlaying isolates from soil and iChips with ESKAPE indicator species (2b). Molecular work included DNA extraction directly from soil samples, and from bacterial colonies recovered from soil dilution plating and isolation chips (iChips) (2c), PCR amplification of 16S rRNA genes and electrophoretic evaluation of PCR products (2d), followed by high throughput amplicon sequencing using the MiSeq platform (2e). Outcomes of the project were assessed through data analysis using QIIME (3a) and the production of a written report (3b)

    Journal: Ecology and Evolution

    Article Title: A practical introduction to microbial molecular ecology through the use of isolation chips. A practical introduction to microbial molecular ecology through the use of isolation chips

    doi: 10.1002/ece3.4748

    Figure Lengend Snippet: Experimental design of molecular microbial ecology group project. The 18‐week project was divided into three parts: (1) field work, (2) lab work, (3) data analysis and reporting. During fieldwork, students were provided with iChips that they loaded with soil dilutions (1a) and then buried in dedicated locations for 2 weeks (1b). Initial lab work included preparation of soil extract agar plates, plating soil dilutions, and incubated iChip wells (2a) and overlaying isolates from soil and iChips with ESKAPE indicator species (2b). Molecular work included DNA extraction directly from soil samples, and from bacterial colonies recovered from soil dilution plating and isolation chips (iChips) (2c), PCR amplification of 16S rRNA genes and electrophoretic evaluation of PCR products (2d), followed by high throughput amplicon sequencing using the MiSeq platform (2e). Outcomes of the project were assessed through data analysis using QIIME (3a) and the production of a written report (3b)

    Article Snippet: 2.7.2 Library preparation Illumina libraries were prepared using a Nextera XT kit, following the manufacturer's recommendations for 16S rRNA gene PCR amplicon barcoding (using 2× NEBNext High‐Fidelity PCR master mix), clean up and pooling.

    Techniques: Incubation, DNA Extraction, Isolation, Polymerase Chain Reaction, Amplification, High Throughput Screening Assay, Sequencing