Journal: Journal of Bacteriology
Article Title: Atypical 16S rRNA Gene Copies in Ochrobactrum intermedium Strains Reveal a Large Genomic Rearrangement by Recombination between rrn Copies
Figure Lengend Snippet: PFGE migration of undigested and I- Ceu I-digested genomic DNAs of O. intermedium strains ADV1 and ADV3. (A) Migration of high-molecular-weight fragments. Lanes 1 and 2, undigested DNA from strain ADV1 (lane 1) and strain ADV3 (lane 2); lanes 3 and 4, I- Ceu I-digested DNA from strain ADV1 (lane 3) and strain ADV3 (lane 4); Lanes Sc and Sp , Saccharomyces cerevisiae ( Sc ) and Schizosaccharomyces pombe ( Sp ) chromosomes (Bio-Rad) as molecular size markers. (B) Migration of low-molecular-weight I- Ceu I fragments. Lane 1, strain ADV1; lane 2, strain ADV3. Chromosomes and I- Ceu I digestion patterns of O. intermedium strains ADV2 and ADV4 to -7 are identical to patterns for strains ADV1 and ADV3, respectively (data not shown). A mixture of λ digested by Hin dIII, the λ concatemer, and Saccharomyces cerevisiae chromosomes was used as the molecular size marker (lane λ/ Sc ); the bands useful for the measure were, from the bottom, 27, 50, 100, 150, 200, 225, 250, and 285 kb.
Article Snippet: The copy number of 16S rRNA gene was determined by a Southern blotting experiment, using a 16S rDNA probe, on genomic DNAs digested by Hin dIII (restriction site absent from the 16S rDNA in the genus Ochrobactrum ).
Techniques: Migration, Molecular Weight, Marker