Journal: Cardiology Journal

Article Title: High mobility group box-1 in hypothalamic paraventricular nuclei attenuates sympathetic tone in rats at post-myocardial infarction

doi: 10.5603/CJ.a2018.0117

Figure Lengend Snippet: Norepinephrine content in serum of rats. ELISA results of the NE content in serum obtained from rats in the control group, myocardial infarction (MI) group and MI+Anti-HMGB1 polyclonal antibody group. Data are expressed as mean ± standard deviation.

Article Snippet: ELISA A rat noradrenaline (NA) ELISA Kit (Catalog Number CSB-E07022r, CUSABIO) was used to detect serum NE concentrations, according to manufacturer instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Saikosaponin A protects against experimental sepsis via inhibition of NOD2-mediated NF-κB activation

doi: 10.3892/etm.2015.2558

Figure Lengend Snippet: Intestinal concentrations of TNF-α and IL-6 measured by ELISA. Intestinal concentrations of (A) TNF-α in sham (control) and (B) CLP group rats at 1, 2, 4, 6 and 8 h following CLP surgery. Results are presented as the means ± standard deviation of three independent experiments. TNF, tumor necrosis factor; IL, interleukin; CLP, cecal ligation and puncture.

Article Snippet: TNF-α and IL-6 levels in the tissue homogenates were determined by ELISA using commercial kits (TNF-α ELISA kit; Diaclone, Besançon, France; IL-6 Rat ELISA kit, cat. no. KRC0061; BioSource Europe SA, Nivelles, Belgium).

Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation, Ligation

Journal: Experimental and Therapeutic Medicine

Article Title: Saikosaponin A protects against experimental sepsis via inhibition of NOD2-mediated NF-κB activation

doi: 10.3892/etm.2015.2558

Figure Lengend Snippet: Effects of SsA on the levels of TNF-α and IL-6. Effects of SsA at different doses on the concentrations of (A) TNF-α and (B) IL-6 in rat intestines at 8 h after CLP or sham surgery. Results are presented as the means ± standard deviation of three independent experiments. *P<0.05 compared with the sham group; #P<0.05 compared with the CLP group. SsA, saikosaponin A; TNF, tumor necrosis factor; IL, interleukin; CLP, cecal ligation and puncture.

Article Snippet: TNF-α and IL-6 levels in the tissue homogenates were determined by ELISA using commercial kits (TNF-α ELISA kit; Diaclone, Besançon, France; IL-6 Rat ELISA kit, cat. no. KRC0061; BioSource Europe SA, Nivelles, Belgium).

Techniques: Standard Deviation, Ligation

Journal: BMC Research Notes

Article Title: A small scale study on the effects of oral administration of the β-glucan produced by Aureobasidium pullulans on milk quality and cytokine expressions of Holstein cows, and on bacterial flora in the intestines of Japanese black calves

doi: 10.1186/1756-0500-5-189

Figure Lengend Snippet: The effects of oral administration of the β-(1 → 3),(1 → 6)-D-glucan on cytokine expressions in the serum of Holstein cows. Serum was sampled from the Holstein cows used in Figure , and then the expressions of tumor necrosis factor-α (TNF-α; A ), interleukin (IL)-6 (B) , and IL-8 (C) in the serum were quantified by ELISA. The bar graphs indicate the quantification value of each serum, and the line graphs show the mean.

Article Snippet: After the serum was separated by centrifugation, the serum expression levels of interleukin (IL)-6, interleukin-8 (also called CXCL-8) and TNF-α (tumor necrosis factor-α) were analyzed using commercially available ELISA kits (Cusabio Biotech, Wuhan, China) in accordance with the manufacturer's protocols.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Fibroblast growth factor 2 protects against renal ischaemia/reperfusion injury by attenuating mitochondrial damage and proinflammatory signalling

doi: 10.1111/jcmm.13203

Figure Lengend Snippet: FGF2 inhibits I/RI‐induced HMGB1 serum release and inflammatory response. Animals were divided into 5 groups ( n = 4), including sham‐operated control, I/RI group, and I/RI with FGF2 pre‐treatment or delayed treatment at 1 and 12 hrs, respectively, after reperfusion as indicated. The samples were collected at 48 hrs following reperfusion for Western blot, ELISA, Immunohistochemistry staining (IHC) and qRT‐PCR analysis as detailed below. ( A ) Western blot analysis to determine the expression of HMGB1 and TNFα in renal tissues with GAPDH as loading control. ( B ) ELISA assay was used to determine the levels of HMGB1 in the serum of animals receiving indicated treatments. ** P < 0.01 versus sham group, ## P < 0.01 versus I/R group. ( C ) IHC of kidney tissue sections for expression of HMGB1. Original magnification ×20. One representative area of renal tissue staining from 1 of 4 animals in each group is shown. (D) Real‐time PCR quantification of mRNA levels for KIM1, TLR2, TLR4, IL‐1α, IL‐6 and TNFα in the kidney, respectively. The result is normalized to GAPDH. The data are presented as mean ± S.E. ( n = 4). *** P < 0.001, ** P < 0.001 versus sham group; ### P < 0.001, # P < 0.05 versus I/R group.

Article Snippet: Rat HMGB1 ELISA Kit was purchased from CUSABIO (Hubei, China).

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining, Quantitative RT-PCR, Expressing, Real-time Polymerase Chain Reaction