Journal: The Journal of Clinical Investigation
Article Title: Elevated circulating amyloid concentrations in obesity and diabetes promote vascular dysfunction
Figure Lengend Snippet: Loss of BACE1 prevents HF feeding–induced endothelial and vascular smooth muscle dysfunction. ( A ) Endothelium-dependent microvascular responses induced by ACh in RC-fed WT, DIO (20-week HF-fed) mice, and RC- and HF-fed BACE1 -KO mice ( n = 10–21). PE, phenylephrine. ( B ) Quantitative analysis of ACh response from A . ( C ) Endothelium-independent responses induced by SNP in RC-WT, DIO, and BACE1 -KO mice ( n = 8–9). ( D ) Quantitative analysis of SNP responses from C . ( E ) Microvascular responses induced by ACh in the presence of L-NAME in RC-fed WT, DIO, and RC- and HF-fed WT and BACE1 -KO mice ( n = 6–10). ( F ) Quantitative analysis of ACh responses from E . The broken line denotes the RC-fed WT ACh response. ( G ) Microvascular responses to localized heating in RC-fed WT, DIO, and RC- and HF-fed BACE1 -KO mice ( n = 9–11). ( H ) Quantitative analysis of heating responses from G . Data are means ± SEM. * P
Article Snippet: Assessment of microvascular function in mice.Two days before the measurements, hair was removed from the flank area of the mouse using a combination of shaving and commercially available depilatory cream (Nair).
Techniques: Mouse Assay