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Image Search Results

Journal: Frontiers in Endocrinology
Article Title: Phloretin Prevents Diabetic Cardiomyopathy by Dissociating Keap1/Nrf2 Complex and Inhibiting Oxidative Stress
doi: 10.3389/fendo.2018.00774
Figure Lengend Snippet: PHL inhibited cardiac oxidative stress and fibrosis in the diabetic myocardium. (A) Representative images for DHE staining using the frozen section of heart tissues as described in Materials and Methods (1000 × magnification). (B) Assessment of cardiac fibrosis by Masson's Trichrome staining (400 × magnification). (C–H) The mRNA expression levels of HO-1 (C) , NQO-1 (D) , GCLC (E) , TGF-β (F) , collagen-1 (G) , and CTGF (H) in myocardial tissues of each group were determined by real-time qPCR. Six mice in each group were used for above analysis. * P
Article Snippet: Antibodies for Nrf2 (#12721),
Techniques: Staining, Expressing, Real-time Polymerase Chain Reaction, Mouse Assay

Journal: Frontiers in Endocrinology
Article Title: Phloretin Prevents Diabetic Cardiomyopathy by Dissociating Keap1/Nrf2 Complex and Inhibiting Oxidative Stress
doi: 10.3389/fendo.2018.00774
Figure Lengend Snippet: PHL reduced hyperglycemia-induced hypertrophy and fibrosis in H9c2 cells. H9c2 (1 * 10 6 ) cells were pre-treated with PHL (10 μM) for 1 h and then incubated with HG (33 mM) for 24 h. (A) The cell lysates were immunoblotted for ANP, TGF-β, with GAPDH as a loading control. (B) The cell sizes were detected by Rhodamine Phalloidin/DAPI immunofluorescence staining. Total RNAs were extracted and the mRNA levels of ANP (C) , BNP (D) , β-MyHC (E) , TGF-β (F) , collagen-1 (G) , and CTGF (H) were detected by RT-qPCR. Data are presented as mean ± SEM. * P
Article Snippet: Antibodies for Nrf2 (#12721),
Techniques: Incubation, Aqueous Normal-phase Chromatography, Immunofluorescence, Staining, Quantitative RT-PCR