rapid dna ligation kit Search Results


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  • 96
    Thermo Fisher rapid dna ligation
    SCIPay design permits CRISPR-guided integration of specific donor <t>DNA</t> sequences via homology directed repair. (A) Overview of SCIPay design. Here, HA present in the payload sequence lead to its genomic integration via HDR. (B – G) EL4 cells were electroporated with SCIPay containing a <t>BFP</t> transgene targeting B2M with various HA lengths. (B) Percentage of knock-out cells. (C) Efficiency and (D) precision scores for GFP, denoting integration of the non-payload plasmid sequence. (E) Efficiency and (F) precision scores for BFP, denoting HDR integration of BFP. (G) Ratio of BFP-to GFP-positive cells. (H) Representative flow cytometry plots. Results in (B – G) represent means +/- SEM of 4 independent experiments. Data was analyzed using 1-way ANOVA and significant differences are indicated with lower case letters, where groups with different letters are significantly different than each other (p
    Rapid Dna Ligation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid dna ligation/product/Thermo Fisher
    Average 96 stars, based on 448 article reviews
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    rapid dna ligation - by Bioz Stars, 2020-08
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    93
    Millipore rapid dna ligation kit
    SCIPay design permits CRISPR-guided integration of specific donor <t>DNA</t> sequences via homology directed repair. (A) Overview of SCIPay design. Here, HA present in the payload sequence lead to its genomic integration via HDR. (B – G) EL4 cells were electroporated with SCIPay containing a <t>BFP</t> transgene targeting B2M with various HA lengths. (B) Percentage of knock-out cells. (C) Efficiency and (D) precision scores for GFP, denoting integration of the non-payload plasmid sequence. (E) Efficiency and (F) precision scores for BFP, denoting HDR integration of BFP. (G) Ratio of BFP-to GFP-positive cells. (H) Representative flow cytometry plots. Results in (B – G) represent means +/- SEM of 4 independent experiments. Data was analyzed using 1-way ANOVA and significant differences are indicated with lower case letters, where groups with different letters are significantly different than each other (p
    Rapid Dna Ligation Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid dna ligation kit/product/Millipore
    Average 93 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    rapid dna ligation kit - by Bioz Stars, 2020-08
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    94
    Roche rapid dna ligation kit
    Schematic representation of the expression constructs ( A ) pLgals1-hum-β3GN-T2 and ( B ) pLgals1-hum-MFng . The <t>PCR-amplified</t> <t>DNA</t> sequences were inserted in the pLgals1-Tev vector construct at the multicloning site, which is preceded by the 6HisTag
    Rapid Dna Ligation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 3334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid dna ligation kit/product/Roche
    Average 94 stars, based on 3334 article reviews
    Price from $9.99 to $1999.99
    rapid dna ligation kit - by Bioz Stars, 2020-08
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    92
    Boehringer Mannheim rapid dna ligation kit
    Schematic representation of the expression constructs ( A ) pLgals1-hum-β3GN-T2 and ( B ) pLgals1-hum-MFng . The <t>PCR-amplified</t> <t>DNA</t> sequences were inserted in the pLgals1-Tev vector construct at the multicloning site, which is preceded by the 6HisTag
    Rapid Dna Ligation Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid dna ligation kit/product/Boehringer Mannheim
    Average 92 stars, based on 154 article reviews
    Price from $9.99 to $1999.99
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    85
    Promega rapid dna ligation kits
    Schematic representation of the expression constructs ( A ) pLgals1-hum-β3GN-T2 and ( B ) pLgals1-hum-MFng . The <t>PCR-amplified</t> <t>DNA</t> sequences were inserted in the pLgals1-Tev vector construct at the multicloning site, which is preceded by the 6HisTag
    Rapid Dna Ligation Kits, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid dna ligation kits/product/Promega
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rapid dna ligation kits - by Bioz Stars, 2020-08
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    92
    Beyotime rapid dna ligation kit
    Schematic representation of the expression constructs ( A ) pLgals1-hum-β3GN-T2 and ( B ) pLgals1-hum-MFng . The <t>PCR-amplified</t> <t>DNA</t> sequences were inserted in the pLgals1-Tev vector construct at the multicloning site, which is preceded by the 6HisTag
    Rapid Dna Ligation Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid dna ligation kit/product/Beyotime
    Average 92 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
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    95
    Promega rapid dna ligation kit
    Crystals of MERS-CoV <t>nsp13.</t> Nsp13 crystallized by incubation with 5′-triphosphate-15T <t>DNA</t> at 18°C using the hanging-drop vapor-diffusion system. The crystals are grown to the biggest in a week
    Rapid Dna Ligation Kit, supplied by Promega, used in various techniques. Bioz Stars score: 95/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid dna ligation kit/product/Promega
    Average 95 stars, based on 72 article reviews
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    85
    Roche rapid dna ligation kits
    Crystals of MERS-CoV <t>nsp13.</t> Nsp13 crystallized by incubation with 5′-triphosphate-15T <t>DNA</t> at 18°C using the hanging-drop vapor-diffusion system. The crystals are grown to the biggest in a week
    Rapid Dna Ligation Kits, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid dna ligation kits/product/Roche
    Average 85 stars, based on 17 article reviews
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    rapid dna ligation kits - by Bioz Stars, 2020-08
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    88
    Roche rapid dna dephos ligation kit
    Crystals of MERS-CoV <t>nsp13.</t> Nsp13 crystallized by incubation with 5′-triphosphate-15T <t>DNA</t> at 18°C using the hanging-drop vapor-diffusion system. The crystals are grown to the biggest in a week
    Rapid Dna Dephos Ligation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific ligafast rapid dna ligation kit
    Crystals of MERS-CoV <t>nsp13.</t> Nsp13 crystallized by incubation with 5′-triphosphate-15T <t>DNA</t> at 18°C using the hanging-drop vapor-diffusion system. The crystals are grown to the biggest in a week
    Ligafast Rapid Dna Ligation Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Roche rapid ligation kit
    Crystals of MERS-CoV <t>nsp13.</t> Nsp13 crystallized by incubation with 5′-triphosphate-15T <t>DNA</t> at 18°C using the hanging-drop vapor-diffusion system. The crystals are grown to the biggest in a week
    Rapid Ligation Kit, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rapid ligation kit/product/Roche
    Average 90 stars, based on 587 article reviews
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    Image Search Results


    SCIPay design permits CRISPR-guided integration of specific donor DNA sequences via homology directed repair. (A) Overview of SCIPay design. Here, HA present in the payload sequence lead to its genomic integration via HDR. (B – G) EL4 cells were electroporated with SCIPay containing a BFP transgene targeting B2M with various HA lengths. (B) Percentage of knock-out cells. (C) Efficiency and (D) precision scores for GFP, denoting integration of the non-payload plasmid sequence. (E) Efficiency and (F) precision scores for BFP, denoting HDR integration of BFP. (G) Ratio of BFP-to GFP-positive cells. (H) Representative flow cytometry plots. Results in (B – G) represent means +/- SEM of 4 independent experiments. Data was analyzed using 1-way ANOVA and significant differences are indicated with lower case letters, where groups with different letters are significantly different than each other (p

    Journal: bioRxiv

    Article Title: Self-cutting and integrating CRISPR plasmids (SCIPs) enable targeted genomic integration of large genetic payloads for rapid cell engineering

    doi: 10.1101/2020.03.25.008276

    Figure Lengend Snippet: SCIPay design permits CRISPR-guided integration of specific donor DNA sequences via homology directed repair. (A) Overview of SCIPay design. Here, HA present in the payload sequence lead to its genomic integration via HDR. (B – G) EL4 cells were electroporated with SCIPay containing a BFP transgene targeting B2M with various HA lengths. (B) Percentage of knock-out cells. (C) Efficiency and (D) precision scores for GFP, denoting integration of the non-payload plasmid sequence. (E) Efficiency and (F) precision scores for BFP, denoting HDR integration of BFP. (G) Ratio of BFP-to GFP-positive cells. (H) Representative flow cytometry plots. Results in (B – G) represent means +/- SEM of 4 independent experiments. Data was analyzed using 1-way ANOVA and significant differences are indicated with lower case letters, where groups with different letters are significantly different than each other (p

    Article Snippet: This modular plasmid allows insertion of gRNA-coding DNA oligonucleotides using BpiI as outlined above and subsequent cloning of HA- and bait-coding DNA fragments engineered to be in-frame with the preceding genome sequence and following P2A-BFP sequence with BsaI using Gibson or restriction/ligation cloning.

    Techniques: CRISPR, Sequencing, Knock-Out, Plasmid Preparation, Flow Cytometry

    Schematic representation of the expression constructs ( A ) pLgals1-hum-β3GN-T2 and ( B ) pLgals1-hum-MFng . The PCR-amplified DNA sequences were inserted in the pLgals1-Tev vector construct at the multicloning site, which is preceded by the 6HisTag

    Journal: Glycobiology

    Article Title: The N-acetyl-binding pocket of N-acetylglucosaminyltransferases also accommodates a sugar analog with a chemical handle at C2

    doi: 10.1093/glycob/cwr110

    Figure Lengend Snippet: Schematic representation of the expression constructs ( A ) pLgals1-hum-β3GN-T2 and ( B ) pLgals1-hum-MFng . The PCR-amplified DNA sequences were inserted in the pLgals1-Tev vector construct at the multicloning site, which is preceded by the 6HisTag

    Article Snippet: The DNA clones were obtained from Open Biosystems, Huntsville, Alabama and contained the coding sequence for hum-β3GN-T2 (accession number ) and hum-MFng (accession number ); the pLgals1 vector was constructed at our laboratory (25); Rosetta (DE3)pLysS cells were obtained from Novagen; XL2 blue ultra-competent cells from Stratagene; Taq-DNA polymerase, polymerase chain reaction (PCR) nucleotide mix and rapid DNA ligation kit from Roche Pharmaceuticals; DNA miniprep spin columns, PCR purification and low-melting agarose extraction kits from Qiagen; restriction enzymes from New England Biolabs, Inc.; Ampicillin, UDP-GlcNAc, UDP-GalNAc, free GlcNAc and pNP-α-Fuc from Sigma-Aldrich; UDP-[6-3 H]-GalNAc and UDP-[6-3 H]-GlcNAc from American Radiolabeled Chemicals; AG 1-X8 chloride resin 200–400 mesh from Bio-Rad; UDP-agarose gel from Calbiochem.

    Techniques: Expressing, Construct, Polymerase Chain Reaction, Amplification, Plasmid Preparation

    Crystals of MERS-CoV nsp13. Nsp13 crystallized by incubation with 5′-triphosphate-15T DNA at 18°C using the hanging-drop vapor-diffusion system. The crystals are grown to the biggest in a week

    Journal: MERS Coronavirus

    Article Title: Deducing the Crystal Structure of MERS-CoV Helicase

    doi: 10.1007/978-1-0716-0211-9_6

    Figure Lengend Snippet: Crystals of MERS-CoV nsp13. Nsp13 crystallized by incubation with 5′-triphosphate-15T DNA at 18°C using the hanging-drop vapor-diffusion system. The crystals are grown to the biggest in a week

    Article Snippet: Digested nsp13 DNA should be ligated with pFast-bac-6×Histidine-SUMO vector using the rapid DNA ligation kit.

    Techniques: Incubation, Diffusion-based Assay