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Image Search Results

Journal: Scientific Reports
Article Title: Impact of Mycobacterium tuberculosis RD1-locus on human primary dendritic cell immune functions
doi: 10.1038/srep17078
Figure Lengend Snippet: The intracellular pathways involving the activations of p38, p44/42 MAPK (3 h post infection) and of NFKB p65 subunit, IRF3 transcriptional factor (6 h post infection) were investigated by western blotting on whole cell extracts prepared from DC infected with wt Mtb or with the indicated Mtb and BCG strains. To detect the phosphorylated IRF3 isoforms (p-IRF3, upper panel), cell extracts were analyzed on an SDS-10% PAGE gel and subjected to immunoblot analysis with anti-IRF3 Ab, while a shorter electrophoretic run on an SDS-10% PAGE gel was performed to analyze the relative levels of IRF3 content (lower panel). A representative experiment, out of 3 independent experiments performed, is shown.
Article Snippet: Blots were incubated with rabbit polyclonal anti-IRF3 and
Techniques: Infection, Western Blot