rabbit polyclonal anti p2rx7 extracellular antibody Search Results


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    Alomone Labs anti p2x7 receptor extracellular antibody
    Role of FPR2 and <t>P2X7</t> receptors in senescent and non-senescent endothelial cells. (A) Non-senescent HUVECs were preincubated with WRW4 (0.1 or 1 µ M) or KN-62 (0.1 or 1 µ M) for 30 min, and then incubated with LL-37 (5 µ g/ml) for 24 h. Alternatively, non-senescent HUVECs were preincubated with a combination of WRW4 (0.1 µ M) and KN-62 (0.1 µ M) for 30 min, and then incubated with LL-37 (5 µ g/ml) for 24 h. ICAM-1 protein expression levels were analyzed by western blotting. Relative expression of ICAM-1/GAPDH was calculated as a ratio to LL-37-stimulated cells without antagonists. Data are presented as the mean ± SD of at least three independent experiments. (B) Cell surface expression levels of LL-37 receptors FPR2 and (C) P2X7 were analyzed in senescent and non-senescent HUVECs by flow cytometry. Relative expression in senescent cells was calculated as a ratio to non-senescent cells. Data are presented as the mean ± SD of at least four independent experiments. * P
    Anti P2x7 Receptor Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Role of FPR2 and P2X7 receptors in senescent and non-senescent endothelial cells. (A) Non-senescent HUVECs were preincubated with WRW4 (0.1 or 1 µ M) or KN-62 (0.1 or 1 µ M) for 30 min, and then incubated with LL-37 (5 µ g/ml) for 24 h. Alternatively, non-senescent HUVECs were preincubated with a combination of WRW4 (0.1 µ M) and KN-62 (0.1 µ M) for 30 min, and then incubated with LL-37 (5 µ g/ml) for 24 h. ICAM-1 protein expression levels were analyzed by western blotting. Relative expression of ICAM-1/GAPDH was calculated as a ratio to LL-37-stimulated cells without antagonists. Data are presented as the mean ± SD of at least three independent experiments. (B) Cell surface expression levels of LL-37 receptors FPR2 and (C) P2X7 were analyzed in senescent and non-senescent HUVECs by flow cytometry. Relative expression in senescent cells was calculated as a ratio to non-senescent cells. Data are presented as the mean ± SD of at least four independent experiments. * P

    Journal: International Journal of Molecular Medicine

    Article Title: Bacterial lipopolysaccharide and antimicrobial LL-37 enhance ICAM-1 expression and NF-κB p65 phosphorylation in senescent endothelial cells

    doi: 10.3892/ijmm.2019.4294

    Figure Lengend Snippet: Role of FPR2 and P2X7 receptors in senescent and non-senescent endothelial cells. (A) Non-senescent HUVECs were preincubated with WRW4 (0.1 or 1 µ M) or KN-62 (0.1 or 1 µ M) for 30 min, and then incubated with LL-37 (5 µ g/ml) for 24 h. Alternatively, non-senescent HUVECs were preincubated with a combination of WRW4 (0.1 µ M) and KN-62 (0.1 µ M) for 30 min, and then incubated with LL-37 (5 µ g/ml) for 24 h. ICAM-1 protein expression levels were analyzed by western blotting. Relative expression of ICAM-1/GAPDH was calculated as a ratio to LL-37-stimulated cells without antagonists. Data are presented as the mean ± SD of at least three independent experiments. (B) Cell surface expression levels of LL-37 receptors FPR2 and (C) P2X7 were analyzed in senescent and non-senescent HUVECs by flow cytometry. Relative expression in senescent cells was calculated as a ratio to non-senescent cells. Data are presented as the mean ± SD of at least four independent experiments. * P

    Article Snippet: Polyclonal anti-P2X7 (cat. no. APR-008; Alomone Labs), normal rabbit IgG (cat. no. PM035; Medical and Biological Laboratories, Ltd.) and PE-conjugated donkey anti-rabbit IgG (cat. no. 406421; BioLegend, Inc.) were also used for flow cytometry.

    Techniques: Incubation, Expressing, Western Blot, Flow Cytometry

    P2X7 constitutes the low-affinity spermatogonial ATP sensor. (A) Exemplary whole-cell voltage-clamp recordings of currents triggered by repetitive stimulation (1 s; ISI = 3 s; S 1 , S 6 ) with ATP (300 µM; left) or BzATP (300 µM; right), respectively. Traces from untransfected spermatogonia (black) and cells transfected with either nontarget negative control siRNA (gray) or a construct targeting P2X4 (green) are overlaid. (B) Quantification of results from repetitive stimulation experiments as shown in A. Peak current densities (means ± SEM) are color coded and plotted as a function of stimulus repetition. Cells were analyzed under control conditions ( n = 33/13 [ATP/BzATP]; black), after transfection with nontargeting siRNA ( n = 16/23 [ATP/BzATP]; gray), and after P2X4 knockdown ( n = 16/23 [ATP/BzATP]; green). Inset (left) shows the data delimited by the dashed rectangle at an increased scale. Asterisks (*) indicate statistical significance, P

    Journal: The Journal of General Physiology

    Article Title: Distinct purinergic signaling pathways in prepubescent mouse spermatogonia

    doi: 10.1085/jgp.201611636

    Figure Lengend Snippet: P2X7 constitutes the low-affinity spermatogonial ATP sensor. (A) Exemplary whole-cell voltage-clamp recordings of currents triggered by repetitive stimulation (1 s; ISI = 3 s; S 1 , S 6 ) with ATP (300 µM; left) or BzATP (300 µM; right), respectively. Traces from untransfected spermatogonia (black) and cells transfected with either nontarget negative control siRNA (gray) or a construct targeting P2X4 (green) are overlaid. (B) Quantification of results from repetitive stimulation experiments as shown in A. Peak current densities (means ± SEM) are color coded and plotted as a function of stimulus repetition. Cells were analyzed under control conditions ( n = 33/13 [ATP/BzATP]; black), after transfection with nontargeting siRNA ( n = 16/23 [ATP/BzATP]; gray), and after P2X4 knockdown ( n = 16/23 [ATP/BzATP]; green). Inset (left) shows the data delimited by the dashed rectangle at an increased scale. Asterisks (*) indicate statistical significance, P

    Article Snippet: Primary antibodies were anti-DAZL (1:500; Abcam), anti-Sloα1 (extracellular; 1:500; Alomone Labs), anti-P2X4 (extracellular; 1:100, Alomone Labs), and anti-P2X7 (extracellular; 1:500; Alomone Labs).

    Techniques: Transfection, Negative Control, Construct

    P2X4 is a candidate mediator of the high-affinity ATP response in spermatogonia. (A) Reverse transcription PCR reveals transcripts for P2rx2 , P2rx4 , and P2rx7 in both Sertoli–germ cell co-cultures and whole testis preparations at P7. A faint band for P2rx3 -encoding cDNA is only amplified from whole testis samples but not co-cultures. Primer pairs generate amplificates of 150–250 bp, respectively. cDNA from whole brain/spinal cord serves as positive control, whereas no template controls are devoid of detectable signals. (B–E) Exemplary whole-cell voltage-clamp recordings of ATP-induced currents (10 µM; 1 s; V hold = −80 mV; S 1 , S 6 ) under control conditions and during treatment with suramin (10 µM and 100 µM; B), extracellular Cu 2+ (100 µM; C), reduced pH (6.3; D), and ivermectin (3 µM; E). Altered conditions were established for at least 60 s (preincubation). (F) Bar diagram (means ± SEM) depicting the quantitative analysis of experiments exemplified in B–E. Note the discontinuous ordinate (//). Numbers of cells tested are indicated above bars. Asterisks (*) denote statistical significance, P ≤ 0.01 (paired t test).

    Journal: The Journal of General Physiology

    Article Title: Distinct purinergic signaling pathways in prepubescent mouse spermatogonia

    doi: 10.1085/jgp.201611636

    Figure Lengend Snippet: P2X4 is a candidate mediator of the high-affinity ATP response in spermatogonia. (A) Reverse transcription PCR reveals transcripts for P2rx2 , P2rx4 , and P2rx7 in both Sertoli–germ cell co-cultures and whole testis preparations at P7. A faint band for P2rx3 -encoding cDNA is only amplified from whole testis samples but not co-cultures. Primer pairs generate amplificates of 150–250 bp, respectively. cDNA from whole brain/spinal cord serves as positive control, whereas no template controls are devoid of detectable signals. (B–E) Exemplary whole-cell voltage-clamp recordings of ATP-induced currents (10 µM; 1 s; V hold = −80 mV; S 1 , S 6 ) under control conditions and during treatment with suramin (10 µM and 100 µM; B), extracellular Cu 2+ (100 µM; C), reduced pH (6.3; D), and ivermectin (3 µM; E). Altered conditions were established for at least 60 s (preincubation). (F) Bar diagram (means ± SEM) depicting the quantitative analysis of experiments exemplified in B–E. Note the discontinuous ordinate (//). Numbers of cells tested are indicated above bars. Asterisks (*) denote statistical significance, P ≤ 0.01 (paired t test).

    Article Snippet: Primary antibodies were anti-DAZL (1:500; Abcam), anti-Sloα1 (extracellular; 1:500; Alomone Labs), anti-P2X4 (extracellular; 1:100, Alomone Labs), and anti-P2X7 (extracellular; 1:500; Alomone Labs).

    Techniques: Polymerase Chain Reaction, Amplification, Positive Control