rabbit polyclonal anti h2b k120ub Search Results


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    • anti h2b k120 ub  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc anti h2b k120 ub
    Anti H2b K120 Ub, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h2b k120 ub/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h2b k120 ub - by Bioz Stars, 2023-09
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    anti flag  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc anti flag
    Anti Flag, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti flag/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    anti flag - by Bioz Stars, 2023-09
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    protein g agarose beads  (Cytiva Europe)
    86
    Cytiva Europe protein g agarose beads
    Protein G Agarose Beads, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein g agarose beads/product/Cytiva Europe
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    protein g agarose beads - by Bioz Stars, 2023-09
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    monoclonal mouse anti human β actin  (Millipore)
    86
    Millipore monoclonal mouse anti human β actin
    Metformin activates AMPK and suppresses histone H2B monoubiquitination and downstream gene transcription. (A) The T47D cells were treated with 4mM metformin for 12 h, and then the cells were lysed and immnoblotted to determine AMPKα1 T172 phosphorylation and H2B K120 monoubiquitination. <t>β-actin</t> was used as a loading control. (B) The T47D cells were treated with 4mM metformin for 12 h, then mRNA was extracted and the transcription level of p21, cyclin D1 and Tulp4 were examined by quantitative (q)PCR. Data are presented as the mean ± standard deviation (n=3). DMSO, dimethyl sulfoxide; AMPK, 5′-adenosine monophosphate-activated protein kinase.
    Monoclonal Mouse Anti Human β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse anti human β actin/product/Millipore
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    monoclonal mouse anti human β actin - by Bioz Stars, 2023-09
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    anti h4k4me3  (Active Motif)
    97
    Active Motif anti h4k4me3
    Metformin activates AMPK and suppresses histone H2B monoubiquitination and downstream gene transcription. (A) The T47D cells were treated with 4mM metformin for 12 h, and then the cells were lysed and immnoblotted to determine AMPKα1 T172 phosphorylation and H2B K120 monoubiquitination. <t>β-actin</t> was used as a loading control. (B) The T47D cells were treated with 4mM metformin for 12 h, then mRNA was extracted and the transcription level of p21, cyclin D1 and Tulp4 were examined by quantitative (q)PCR. Data are presented as the mean ± standard deviation (n=3). DMSO, dimethyl sulfoxide; AMPK, 5′-adenosine monophosphate-activated protein kinase.
    Anti H4k4me3, supplied by Active Motif, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h4k4me3/product/Active Motif
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    anti h4k4me3 - by Bioz Stars, 2023-09
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    Image Search Results


    Metformin activates AMPK and suppresses histone H2B monoubiquitination and downstream gene transcription. (A) The T47D cells were treated with 4mM metformin for 12 h, and then the cells were lysed and immnoblotted to determine AMPKα1 T172 phosphorylation and H2B K120 monoubiquitination. β-actin was used as a loading control. (B) The T47D cells were treated with 4mM metformin for 12 h, then mRNA was extracted and the transcription level of p21, cyclin D1 and Tulp4 were examined by quantitative (q)PCR. Data are presented as the mean ± standard deviation (n=3). DMSO, dimethyl sulfoxide; AMPK, 5′-adenosine monophosphate-activated protein kinase.

    Journal: Oncology Letters

    Article Title: Metformin inhibits histone H2B monoubiquitination and downstream gene transcription in human breast cancer cells

    doi: 10.3892/ol.2014.2158

    Figure Lengend Snippet: Metformin activates AMPK and suppresses histone H2B monoubiquitination and downstream gene transcription. (A) The T47D cells were treated with 4mM metformin for 12 h, and then the cells were lysed and immnoblotted to determine AMPKα1 T172 phosphorylation and H2B K120 monoubiquitination. β-actin was used as a loading control. (B) The T47D cells were treated with 4mM metformin for 12 h, then mRNA was extracted and the transcription level of p21, cyclin D1 and Tulp4 were examined by quantitative (q)PCR. Data are presented as the mean ± standard deviation (n=3). DMSO, dimethyl sulfoxide; AMPK, 5′-adenosine monophosphate-activated protein kinase.

    Article Snippet: Monoclonal rabbit anti-human anti-phospho-acetyl-CoA carboxylase (Ser79) (pACC1), monoclonal rabbit anti-human anti-phospho-AMPKα (Thr172)(p-AMPKα1), monoclonal rabbit anti-human anti-ubiquityl-histone H2B (Lys120) (H2B K120ub) and rabbit monoclonal anti-human anti-AMPKα1 were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), with the exception of monoclonal mouse anti-human β-actin, was purchased from Sigma-Aldrich.

    Techniques: Standard Deviation

    Metformin effect on T47D cell proliferation depends on AMPK. (A) Western blotting showing the AMPKα1 protein level in the T47D cells following transfection with AMPKα1 siRNA. β-actin was used as a loading control. (B) The T47D cells transfected with control siRNA or AMPKα1 siRNA were treated with 4 mM metformin for 12 h, and the cell proliferation was examined by Cell Titer-Blue cell counting. Data are presented as the mean ± standard deviation (n=3). DMSO, dimethyl sulfoxide; AMPK, 5′-adenosine monophosphate-activated protein kinase.

    Journal: Oncology Letters

    Article Title: Metformin inhibits histone H2B monoubiquitination and downstream gene transcription in human breast cancer cells

    doi: 10.3892/ol.2014.2158

    Figure Lengend Snippet: Metformin effect on T47D cell proliferation depends on AMPK. (A) Western blotting showing the AMPKα1 protein level in the T47D cells following transfection with AMPKα1 siRNA. β-actin was used as a loading control. (B) The T47D cells transfected with control siRNA or AMPKα1 siRNA were treated with 4 mM metformin for 12 h, and the cell proliferation was examined by Cell Titer-Blue cell counting. Data are presented as the mean ± standard deviation (n=3). DMSO, dimethyl sulfoxide; AMPK, 5′-adenosine monophosphate-activated protein kinase.

    Article Snippet: Monoclonal rabbit anti-human anti-phospho-acetyl-CoA carboxylase (Ser79) (pACC1), monoclonal rabbit anti-human anti-phospho-AMPKα (Thr172)(p-AMPKα1), monoclonal rabbit anti-human anti-ubiquityl-histone H2B (Lys120) (H2B K120ub) and rabbit monoclonal anti-human anti-AMPKα1 were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), with the exception of monoclonal mouse anti-human β-actin, was purchased from Sigma-Aldrich.

    Techniques: Western Blot, Transfection, Cell Counting, Standard Deviation