Santa Cruz Biotechnology
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Merck KGaA
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Abcam
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Upstate Biotechnology Inc
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Image Search Results

Journal: Journal of Virology
Article Title: Dysregulation of Cellular VRK1, BAF, and Innate Immune Signaling by the Vaccinia Virus B12 Pseudokinase
doi: 10.1128/jvi.00398-22
Figure Lengend Snippet: VRK1 and B12 coinfluence their solubility properties. (A) Immunoblot analyses of detergent-treated fractions from HAP1 or HAP1-VRK1KO cells transduced with empty-vector (CTRL)- or HA-B12-expressing lentiviruses. Immunoblots detecting Ku70 and histone 2B are shown as fractionation controls. (B) Immunoblot analyses of detergent fractions from CV1, HAP1, or A549 cells uninfected or infected with WT VACV (WR) at an MOI of 5 and harvested at 7 hpi. (C) Immunoblot analyses of fractions from A549 cells uninfected or infected with WT or mutB12 virus (MOI = 5), treated with or without 50 μM AraC, and harvested at 7 hpi. Quantitation of VRK1 and Ku70 abundances was performed for each fraction, with each combined sample abundance (soluble + insoluble) set to 1. Three independent replicates were quantified, (*, P < 0.05). A representative immunoblot is shown.
Article Snippet:
Techniques: Solubility, Western Blot, Transduction, Plasmid Preparation, Expressing, Fractionation, Infection, Quantitation Assay

Journal: Journal of Virology
Article Title: Dysregulation of Cellular VRK1, BAF, and Innate Immune Signaling by the Vaccinia Virus B12 Pseudokinase
doi: 10.1128/jvi.00398-22
Figure Lengend Snippet: Antibody sources and dilutions
Article Snippet:
Techniques:

Journal: PLoS ONE
Article Title: Loss of the Histone Pre-mRNA Processing Factor Stem-Loop Binding Protein in Drosophila Causes Genomic Instability and Impaired Cellular Proliferation
doi: 10.1371/journal.pone.0008168
Figure Lengend Snippet: Protein lysates from wt, Slbp 15 , Slbp 10 , and H2aV 810 mutants were obtained from whole 3 rd instar larvae and probed with antibodies to A) H2b and H3, and B) H3K4-me2 and H3K9-me2. β-tubulin was used as a loading control for both panels.
Article Snippet: Polyclonal rabbit C-terminal H3 (1∶3000) (Abcam #1791),
Techniques: