Journal: The Journal of Clinical Investigation
Article Title: RNA-binding protein ZFP36L1 maintains posttranscriptional regulation of bile acid metabolism
Figure Lengend Snippet: (A–C) Hepatic expression of Shp, Zfp36l1, and Cyp7a1 genes in Zfp36l1fl/fl and littermate Zfp36l1L-KO mice treated with either vehicle (n = 10, Zfp36l1fl/fl; n = 13, Zfp36l1L-KO) or GSK2324 for 30 minutes (n = 8, Zfp36l1fl/fl; n = 7, Zfp36l1L-KO), 1 hour (n = 8, Zfp36l1fl/fl; n = 8, Zfp36l1L-KO), or 2 hours (n = 8, Zfp36l1fl/fl; n = 7, Zfp36l1L-KO) before sacrifice. Gene expression analysis was determined by qRT-PCR and normalized to Tbp. (D) Cyp7a1 levels after GSK treatment in Zfp36l1fl/fl and Zfp36l1L-KO mice compared with levels in vehicle-treated mice, set to 1 (blue lines represent the same data as in C). (E) Summary diagram depicting known FXR-regulated pathways that control bile acid synthesis feedback inhibition. Data represent the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001, by 1-way ANOVA.
Article Snippet: Anti-ZFP36L1 antibody (BRF1/2, catalog 2119; Cell Signaling Technology; 1:1,000); anti-CYP7A1 antibody (catalog MABD42; Sigma-Aldrich; 1:1,000); protein disulfide isomerase (PDI) (catalog 3501; Cell Signaling Technology; 1:1,000); and secondary anti-rabbit HRP-conjugated antibody (GE Healthcare; 1:10,000) were used.
Techniques: Expressing, Quantitative RT-PCR, Inhibition