rabbit monoclonal eno1 Search Results


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  • 86
    Abcam rabbit monoclonal eno1
    Source, application and concentration of antibodies.
    Rabbit Monoclonal Eno1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal eno1/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal eno1 - by Bioz Stars, 2024-02
    86/100 stars
      Buy from Supplier

    86
    Abcam monoclonal rabbit anti eno1
    Source, application and concentration of antibodies.
    Monoclonal Rabbit Anti Eno1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal rabbit anti eno1/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal rabbit anti eno1 - by Bioz Stars, 2024-02
    86/100 stars
      Buy from Supplier

    86
    Abcam rabbit monoclonal anti eno1
    Evaluation of HIF-1α function in SF under normoxic conditions. ( a ) Titration of HIF-1α silencing in SF at 7 days after HIF-1α or control (CTRL) siRNA lentiviral transduction after treatment with 300 μM CoCl 2 . Western blot image of HIF-1α and β-actin, and densitometric analysis of HIF-1α expression normalized to the β-actin level. ( b ) Silencing efficiency of HIF-1α siRNA lentivirus in normoxia by qRT-PCR (siCTRL mRNA gene/β-actin ratio set to 100%). HIF-1α, PDK1, GAPDH, INHBB, SDHC and SUCLG2 genes were analyzed. Data are mean ± SEM of 6 independent experiments (*p = 0.03, **p = 0.015, Wilcoxon Signed Rank test). ( c ) HIF-1α knockdown down-regulates the expression of GAPDH. Protein expression of glycolytic enzymes GAPDH, PDK1, <t>ENO1</t> and TPI under normoxic conditions measured by western blot in SF after lentiviral transduction with both HIF-1α and non-silencing control (CTRL) siRNA. Protein extracts were obtained 4 days after transduction. Data are mean ± SEM of 7 independent experiments (*p = 0.015, Wilcoxon test).
    Rabbit Monoclonal Anti Eno1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti eno1/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti eno1 - by Bioz Stars, 2024-02
    86/100 stars
      Buy from Supplier

    86
    Danaher Inc eno1 rabbit monoclonal abcam wb
    Evaluation of HIF-1α function in SF under normoxic conditions. ( a ) Titration of HIF-1α silencing in SF at 7 days after HIF-1α or control (CTRL) siRNA lentiviral transduction after treatment with 300 μM CoCl 2 . Western blot image of HIF-1α and β-actin, and densitometric analysis of HIF-1α expression normalized to the β-actin level. ( b ) Silencing efficiency of HIF-1α siRNA lentivirus in normoxia by qRT-PCR (siCTRL mRNA gene/β-actin ratio set to 100%). HIF-1α, PDK1, GAPDH, INHBB, SDHC and SUCLG2 genes were analyzed. Data are mean ± SEM of 6 independent experiments (*p = 0.03, **p = 0.015, Wilcoxon Signed Rank test). ( c ) HIF-1α knockdown down-regulates the expression of GAPDH. Protein expression of glycolytic enzymes GAPDH, PDK1, <t>ENO1</t> and TPI under normoxic conditions measured by western blot in SF after lentiviral transduction with both HIF-1α and non-silencing control (CTRL) siRNA. Protein extracts were obtained 4 days after transduction. Data are mean ± SEM of 7 independent experiments (*p = 0.015, Wilcoxon test).
    Eno1 Rabbit Monoclonal Abcam Wb, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eno1 rabbit monoclonal abcam wb/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eno1 rabbit monoclonal abcam wb - by Bioz Stars, 2024-02
    86/100 stars
      Buy from Supplier

    86
    Abcam rabbit anti eno1 monoclonal antibodies
    <t>ENO1</t> expression in liver cancer tissue and peripheral blood of patients with liver cancer. (A) ENO1 expression in liver cancer tissues (×100). (B) ENO1 was not expressed in benign liver lesions (×100). (C) Serum anti-ENO1 antibody levels in patients with liver cancer were higher than those in patients with benign liver lesions and healthy controls. (D) ENO1 expression in liver cancer tissues (×400). (E) ENO1 was not expressed in benign liver lesion (×400). (F) Receiver operating characteristic curve analysis for liver cancer diagnosis using anti-ENO1 antibody levels. The AUC was 0.741, the sensitivity was 64.3% and the specificity was 85.5%. The red dot represents the cut-off value. **P<0.01; ****P<0.0001 ENO1, <t>α-enolase;</t> AUC, area under the curve.
    Rabbit Anti Eno1 Monoclonal Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti eno1 monoclonal antibodies/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti eno1 monoclonal antibodies - by Bioz Stars, 2024-02
    86/100 stars
      Buy from Supplier

    Image Search Results


    Source, application and concentration of antibodies.

    Journal: PLOS ONE

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

    doi: 10.1371/journal.pone.0291023

    Figure Lengend Snippet: Source, application and concentration of antibodies.

    Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

    Techniques: Concentration Assay, Recombinant, Plasmid Preparation

    Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

    Journal: PLOS ONE

    Article Title: Helicase-like transcription factor (HLTF) -deleted CDX/TME model of colorectal cancer increased transcription of oxidative phosphorylation genes and diverted glycolysis to boost S-glutathionylation in lymphatic intravascular metastatic niches

    doi: 10.1371/journal.pone.0291023

    Figure Lengend Snippet: Summary of site-specific S-glutathionylation identified by 2D-DIGE MALDI-TOF/TOF mass spectrometry. Pr-SSG in HLTF -/- CDX/TME.

    Article Snippet: Rabbit polyclonal HLTF antibody (NBP1-83256) Novus Biologicals (1:100) Rabbit polyclonal CYTB (55090-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal PGAM1 (16126-1-AP) ThermoFisher Scientific (1:50) Rabbit polyclonal ANXA1 (71–3400) ThermoFisher Scientific (1:20) Rabbit monoclonal γH2AX-phospho S139 (ab81299) Abcam (1:50) Rabbit monoclonal ENO1 (ab227978) Abcam (1:2,000) Rabbit recombinant monoclonal mouse-specific PDPN (MA5-29742) ThermoFisher Scientific (1:500) , IHC-P: Vector Laboratories RTU Biotinylated Goat Anti-rabbit IgG (H+L) (BP-9100-50).

    Techniques: Mass Spectrometry

    Evaluation of HIF-1α function in SF under normoxic conditions. ( a ) Titration of HIF-1α silencing in SF at 7 days after HIF-1α or control (CTRL) siRNA lentiviral transduction after treatment with 300 μM CoCl 2 . Western blot image of HIF-1α and β-actin, and densitometric analysis of HIF-1α expression normalized to the β-actin level. ( b ) Silencing efficiency of HIF-1α siRNA lentivirus in normoxia by qRT-PCR (siCTRL mRNA gene/β-actin ratio set to 100%). HIF-1α, PDK1, GAPDH, INHBB, SDHC and SUCLG2 genes were analyzed. Data are mean ± SEM of 6 independent experiments (*p = 0.03, **p = 0.015, Wilcoxon Signed Rank test). ( c ) HIF-1α knockdown down-regulates the expression of GAPDH. Protein expression of glycolytic enzymes GAPDH, PDK1, ENO1 and TPI under normoxic conditions measured by western blot in SF after lentiviral transduction with both HIF-1α and non-silencing control (CTRL) siRNA. Protein extracts were obtained 4 days after transduction. Data are mean ± SEM of 7 independent experiments (*p = 0.015, Wilcoxon test).

    Journal: Scientific Reports

    Article Title: Hif-1α Knockdown Reduces Glycolytic Metabolism and Induces Cell Death of Human Synovial Fibroblasts Under Normoxic Conditions

    doi: 10.1038/s41598-017-03921-4

    Figure Lengend Snippet: Evaluation of HIF-1α function in SF under normoxic conditions. ( a ) Titration of HIF-1α silencing in SF at 7 days after HIF-1α or control (CTRL) siRNA lentiviral transduction after treatment with 300 μM CoCl 2 . Western blot image of HIF-1α and β-actin, and densitometric analysis of HIF-1α expression normalized to the β-actin level. ( b ) Silencing efficiency of HIF-1α siRNA lentivirus in normoxia by qRT-PCR (siCTRL mRNA gene/β-actin ratio set to 100%). HIF-1α, PDK1, GAPDH, INHBB, SDHC and SUCLG2 genes were analyzed. Data are mean ± SEM of 6 independent experiments (*p = 0.03, **p = 0.015, Wilcoxon Signed Rank test). ( c ) HIF-1α knockdown down-regulates the expression of GAPDH. Protein expression of glycolytic enzymes GAPDH, PDK1, ENO1 and TPI under normoxic conditions measured by western blot in SF after lentiviral transduction with both HIF-1α and non-silencing control (CTRL) siRNA. Protein extracts were obtained 4 days after transduction. Data are mean ± SEM of 7 independent experiments (*p = 0.015, Wilcoxon test).

    Article Snippet: Western blots were probed with following antibodies: mouse monoclonal anti-HIF-1α (BD Biosciences Pharmingen, San Jose, CA), mouse monoclonal anti-GAPDH-HRP (Abcam, Cambridge, UK), rabbit monoclonal anti-PDK1 (Abcam), rabbit monoclonal anti-TPI (Abcam), rabbit monoclonal anti-ENO1 (Abcam), rabbit polyclonal anti-caspase-3 (Cell Signaling Technology, Danvers, MA, USA) and anti-β-actin (Sigma-Aldrich).

    Techniques: Titration, Transduction, Western Blot, Expressing, Quantitative RT-PCR

    ENO1 expression in liver cancer tissue and peripheral blood of patients with liver cancer. (A) ENO1 expression in liver cancer tissues (×100). (B) ENO1 was not expressed in benign liver lesions (×100). (C) Serum anti-ENO1 antibody levels in patients with liver cancer were higher than those in patients with benign liver lesions and healthy controls. (D) ENO1 expression in liver cancer tissues (×400). (E) ENO1 was not expressed in benign liver lesion (×400). (F) Receiver operating characteristic curve analysis for liver cancer diagnosis using anti-ENO1 antibody levels. The AUC was 0.741, the sensitivity was 64.3% and the specificity was 85.5%. The red dot represents the cut-off value. **P<0.01; ****P<0.0001 ENO1, α-enolase; AUC, area under the curve.

    Journal: Oncology Letters

    Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

    doi: 10.3892/ol.2020.12003

    Figure Lengend Snippet: ENO1 expression in liver cancer tissue and peripheral blood of patients with liver cancer. (A) ENO1 expression in liver cancer tissues (×100). (B) ENO1 was not expressed in benign liver lesions (×100). (C) Serum anti-ENO1 antibody levels in patients with liver cancer were higher than those in patients with benign liver lesions and healthy controls. (D) ENO1 expression in liver cancer tissues (×400). (E) ENO1 was not expressed in benign liver lesion (×400). (F) Receiver operating characteristic curve analysis for liver cancer diagnosis using anti-ENO1 antibody levels. The AUC was 0.741, the sensitivity was 64.3% and the specificity was 85.5%. The red dot represents the cut-off value. **P<0.01; ****P<0.0001 ENO1, α-enolase; AUC, area under the curve.

    Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

    Techniques: Expressing

     ENO1  expression in pathological tissues.

    Journal: Oncology Letters

    Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

    doi: 10.3892/ol.2020.12003

    Figure Lengend Snippet: ENO1 expression in pathological tissues.

    Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

    Techniques: Expressing

    Comparison of the serum  anti-ENO1  antibody levels among the three groups of participants [P50 (P25-P75)].

    Journal: Oncology Letters

    Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

    doi: 10.3892/ol.2020.12003

    Figure Lengend Snippet: Comparison of the serum anti-ENO1 antibody levels among the three groups of participants [P50 (P25-P75)].

    Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

    Techniques:

    Validation of the siRNA interference effect on ENO1 expression in liver cancer cells. (A) RT-qPCR validation of the effect of siRNA interference on ENO1 expression in HepG2 cells. The results showed that the relative expression levels of the ENO1 gene in the si-1 and si-2 groups were lower than those in the control group. (B) RT-qPCR validation of the effect of siRNA interference on ENO1 expression in Huh7 cells. The results showed that the relative expression levels of the ENO1 gene in the si-1 and si-2 groups were lower than those in the control group. (C) Western blot validation of the effect of siRNA interference on ENO1 expression in HepG2 cells. The results showed that the relative expression levels of the ENO1 protein in HepG2 cells in the si-1 and si-2 groups were significantly lower than those in the control group. (D) Western blot validation of the effect of siRNA interference on ENO1 expression in Huh7 cells. The results showed that the relative expression levels of the ENO1 protein in Huh7 cells in the si-1 and si-2 groups were significantly lower than those in the control group. **P<0.01; ****P<0.0001. siRNA, small interfering RNA; ENO1, α-enolase; si-1, siRNA interference group 1; si-2, siRNA interference group 2; RT-qPCR, reverse transcription-quantitative PCR.

    Journal: Oncology Letters

    Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

    doi: 10.3892/ol.2020.12003

    Figure Lengend Snippet: Validation of the siRNA interference effect on ENO1 expression in liver cancer cells. (A) RT-qPCR validation of the effect of siRNA interference on ENO1 expression in HepG2 cells. The results showed that the relative expression levels of the ENO1 gene in the si-1 and si-2 groups were lower than those in the control group. (B) RT-qPCR validation of the effect of siRNA interference on ENO1 expression in Huh7 cells. The results showed that the relative expression levels of the ENO1 gene in the si-1 and si-2 groups were lower than those in the control group. (C) Western blot validation of the effect of siRNA interference on ENO1 expression in HepG2 cells. The results showed that the relative expression levels of the ENO1 protein in HepG2 cells in the si-1 and si-2 groups were significantly lower than those in the control group. (D) Western blot validation of the effect of siRNA interference on ENO1 expression in Huh7 cells. The results showed that the relative expression levels of the ENO1 protein in Huh7 cells in the si-1 and si-2 groups were significantly lower than those in the control group. **P<0.01; ****P<0.0001. siRNA, small interfering RNA; ENO1, α-enolase; si-1, siRNA interference group 1; si-2, siRNA interference group 2; RT-qPCR, reverse transcription-quantitative PCR.

    Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Small Interfering RNA, Real-time Polymerase Chain Reaction

    Effect of ENO1 siRNA on liver cancer cell proliferation. (A) HepG2 and (B) Huh7 cell proliferation was suppressed after ENO1 siRNA treatment. Results show that 72 h after transfection in the si-1 and si-2 groups, ENO1 siRNA treatment resulted in proliferation inhibition in HepG2 and Huh7 cells. In HepG2 cells, the differences in cell proliferation between the NC and the siRNA-treated groups were statistically significant (P<0.05 vs. si-2 group and P<0.01 vs. si-1 group). Cell proliferation in Huh7 cells was also significantly different. *P<0.05; **P<0.01. ENO1, α-enolase; siRNA, small interfering RNA; si-1, siRNA interference group 1; si-2, siRNA interference group 2; OD, optical density; NC, negative control.

    Journal: Oncology Letters

    Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

    doi: 10.3892/ol.2020.12003

    Figure Lengend Snippet: Effect of ENO1 siRNA on liver cancer cell proliferation. (A) HepG2 and (B) Huh7 cell proliferation was suppressed after ENO1 siRNA treatment. Results show that 72 h after transfection in the si-1 and si-2 groups, ENO1 siRNA treatment resulted in proliferation inhibition in HepG2 and Huh7 cells. In HepG2 cells, the differences in cell proliferation between the NC and the siRNA-treated groups were statistically significant (P<0.05 vs. si-2 group and P<0.01 vs. si-1 group). Cell proliferation in Huh7 cells was also significantly different. *P<0.05; **P<0.01. ENO1, α-enolase; siRNA, small interfering RNA; si-1, siRNA interference group 1; si-2, siRNA interference group 2; OD, optical density; NC, negative control.

    Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

    Techniques: Transfection, Inhibition, Small Interfering RNA, Negative Control

    Effect of ENO1 siRNA on the migration ability of liver cancer cells. (A) Compared with that of the NC group, HepG2 cell migration after ENO1 siRNA treatment became slower in the si-1 and si-2 groups, and the difference was statistically significant at 48 h after treatment. (B) Gap size was measured using ImageJ software and the ratio of gap size after ENO1 siRNA treatment in HepG2 cells was calculated based on the size of the wound at 0 h. (C) Compared with that of the NC group, Huh7 cell migration after ENO1 siRNA treatment became slower in the si-1 and si-2 groups, and the difference was statistically significant at 48 h after treatment. (D) Gap size was measured with the ImageJ software and the ratio of gap size after ENO1 siRNA treatment in Huh7 cell was calculated based on the size of the wound at 0 h. *P<0.05; **P<0.01. ENO1, α-enolase; siRNA, small interfering RNA; NC, negative control; si-1, siRNA interference group 1; si-2, siRNA interference group 2.

    Journal: Oncology Letters

    Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

    doi: 10.3892/ol.2020.12003

    Figure Lengend Snippet: Effect of ENO1 siRNA on the migration ability of liver cancer cells. (A) Compared with that of the NC group, HepG2 cell migration after ENO1 siRNA treatment became slower in the si-1 and si-2 groups, and the difference was statistically significant at 48 h after treatment. (B) Gap size was measured using ImageJ software and the ratio of gap size after ENO1 siRNA treatment in HepG2 cells was calculated based on the size of the wound at 0 h. (C) Compared with that of the NC group, Huh7 cell migration after ENO1 siRNA treatment became slower in the si-1 and si-2 groups, and the difference was statistically significant at 48 h after treatment. (D) Gap size was measured with the ImageJ software and the ratio of gap size after ENO1 siRNA treatment in Huh7 cell was calculated based on the size of the wound at 0 h. *P<0.05; **P<0.01. ENO1, α-enolase; siRNA, small interfering RNA; NC, negative control; si-1, siRNA interference group 1; si-2, siRNA interference group 2.

    Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

    Techniques: Migration, Software, Small Interfering RNA, Negative Control

    Effect of ENO1 siRNA on the invasion and migration abilities of liver cancer cells. Compared with the NC group, the in vitro invasion and migration abilities of (A) HepG2 and (B) Huh7 cells after ENO1 siRNA treatment decreased in the si-1 and si-2 groups, and the differences were statistically significant. Numbers of (C) HepG2 and (D) Huh7 cells were measured with the ImageJ software after ENO1 siRNA treatment. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. ENO1, α-enolase; siRNA, small interfering RNA; NC, negative control; si-1, siRNA interference group 1; si-2, siRNA interference group 2.

    Journal: Oncology Letters

    Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

    doi: 10.3892/ol.2020.12003

    Figure Lengend Snippet: Effect of ENO1 siRNA on the invasion and migration abilities of liver cancer cells. Compared with the NC group, the in vitro invasion and migration abilities of (A) HepG2 and (B) Huh7 cells after ENO1 siRNA treatment decreased in the si-1 and si-2 groups, and the differences were statistically significant. Numbers of (C) HepG2 and (D) Huh7 cells were measured with the ImageJ software after ENO1 siRNA treatment. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. ENO1, α-enolase; siRNA, small interfering RNA; NC, negative control; si-1, siRNA interference group 1; si-2, siRNA interference group 2.

    Article Snippet: Rabbit anti-ENO1 monoclonal antibodies were purchased from Abcam (cat. no. ab85086) for use in immunohistochemistry and western blotting.

    Techniques: Migration, In Vitro, Software, Small Interfering RNA, Negative Control