Journal: International Journal of Molecular Sciences

Article Title: Dysregulation of Lipid Metabolism in Mkp-1 Deficient Mice during Gram-Negative Sepsis

doi: 10.3390/ijms19123904

Figure Lengend Snippet: Liver p38 activity and lipid contents before and after E. coli infection. Mkp-1 +/+ and Mkp-1 −/− mice were either infected i.v. with E. coli at a dose of 2.5 × 10 7 colony forming units (CFU)/g of body weight or injected with PBS. Mice were euthanized after 24 h, and livers were harvested. ( A ) Phospho-p38 levels in the livers of control and E. coli -infected mice. Parts of the livers were homogenized to extract soluble proteins. Protein samples (40 µg) from distinct animals were resolved to detect phospho-p38 by Western blotting, using a polyclonal antibody against phospho-p38. The membranes were stripped and reblotted with p38 antibody to verify comparable loading. Images shown in the left panel are representative results. Bar graph in the right panel represent quantification of phosphor-p38 (p-p38) levels in each groups ( n = 4). Groups marked with distinct letters above the bars indicate significant differences ( p < 0.05, two-way Analysis of variance (ANOVA)); ( B ) Liver triglyceride; ( C ) Total liver lipid; ( D ) Liver cholesterol. Values in B-D represent means ± SE from 10 different animals, and data were analyzed by two-way ANOVA with Tukey post-test to evaluate main and interactive effects. NS: not significant.

Article Snippet: Mouse monoclonal antibodies against Cpt1a, Dgat2, Fasn, Pck1, and Scd1 as well as the rabbit polyclonal antibody against total p38 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Activity Assay, Infection, Injection, Western Blot