rabbit anti-human mct4 Search Results


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    Biorbyt rabbit anti human mct4
    Localisation of MCT1 and <t>MCT4</t> in rat placenta at four different ages during gestation. The staining of rat placenta with antibodies to (a–d) MCT1 and (e–h) MCT4 is shown for (a, e) GD 11; (b, f) GD 14; (c, g) GD 18; and (d, h) GD 20. Maternal blood vessels and cells are indicated with stars (★) and foetal blood vessels and cells are indicated with arrow heads (▸). Original objective 40x, bar 15 μ m.
    Rabbit Anti Human Mct4, supplied by Biorbyt, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human mct4/product/Biorbyt
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human mct4 - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    91
    Novus Biologicals rabbit anti mct4
    sCPE regulates glucose- and lactate transporters, but does not affect gene expression of key metabolic enzymes (A) Immunoblot detection of GLUT1, GLUT3 and <t>MCT4</t> in the lysates of the Neo or sCPE-transfected LNT229 or Tu140 cells upon CPE knockdown. For Tu140, control siRNA (si-mock) was used as negative control. α-tubulin was used as a loading control. The cells were serum-starved for 24h in serum-reduced medium prior to lysis. A representative immunoblot is shown. (B) Immunohistochemical staining of GLUT1 and LDHA in the Neo or sCPE-overexpressing LNT229 cells (20x magnification, scale bar 100μm). (C) Immunohistochemical staining of GLUT1 in the LNT229 spheres (20x magnification, scale bar 100μm). (D, E) qPCR analysis of CPE, Glut1, Glut3 and MCT4 gene expression in the (D) Neo or sCPE-overexpressing LNT229 cells or (E) Tu140 cells upon CPE knockdown. Control siRNA (si-mock) was used as negative control for CPE knockdown. Red dots represent single experiments. Unpaired t-test with Welch's correction. Mean±SEM; n=3 (D: *p=0.0105; E: *p=0.0422). (F, G) qPCR analysis of the (F) glycolytic enzymes (ALDOC, HKII, PFKP, PFKM, LDHA) and (G) enzymes involved in the pentose-phosphate pathway (G6PD, PGD, TALDO1, TKT, LDHB) in the Neo or sCPE-overexpressing LNT229 cells. Red dots represent single experiments. Unpaired t-test with Welch's correction. Mean±SEM; n=3.
    Rabbit Anti Mct4, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mct4/product/Novus Biologicals
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mct4 - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    85
    Santa Cruz Biotechnology mct4 rabbit
    Summary diagram of lactate metabolism in high lactate-consumers vs. low lactate-consumers. The blue gradient represents oxygen diffusion. The cell on the left is hypoxic; the cells on the right are aerobic. Arrow colors correspond to substrates, and arrow size corresponds to relative amount. Hypoxic tumor cells take up glucose (gray “ Glc ”) and produce lactate (dark green “ L ”), leading to higher concentrations of lactate. Lactate may be taken up by the hypoxic tumor cells, but it is not catabolized. Aerobic tumor cells that are high lactate-consumers and likely express high MCT1/low <t>MCT4</t> can take up lactate and catabolize it to alanine (orange “ A ”) and glutamate (blue “ G ”), which will be exported from the cell. With the aerobic high lactate-consumer cell consuming lactate, more glucose can potentially be spared for hypoxic tumor cell use, potentially conferring a survival advantage. Aerobic tumor cells that are low lactate-consumers and likely express low MCT1/high MCT4 take up less lactate than the high lactate-consumers, consequently producing and exporting less alanine and glutamate. Low lactate-consumers utilize more glucose, which will not allow glucose to reach the hypoxic tumor cells ( A ). One proposed strategy for starving hypoxic tumor cells of glucose in a high lactate-consuming tumor is to treat with a MCT1- inhibitor, like CHC. CHC prevents lactate uptake and catabolism in cells, forcing the aerobic high lactate-consumer to use glucose, which starves the hypoxic tumor cell of glucose. Lactate transport out of hypoxic cells is also inhibited, which would also lead to hypoxic cell death ( B ).
    Mct4 Rabbit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mct4 rabbit/product/Santa Cruz Biotechnology
    Average 85 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    mct4 rabbit - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    91
    Bioss mct4 polyclonal antibody
    Summary diagram of lactate metabolism in high lactate-consumers vs. low lactate-consumers. The blue gradient represents oxygen diffusion. The cell on the left is hypoxic; the cells on the right are aerobic. Arrow colors correspond to substrates, and arrow size corresponds to relative amount. Hypoxic tumor cells take up glucose (gray “ Glc ”) and produce lactate (dark green “ L ”), leading to higher concentrations of lactate. Lactate may be taken up by the hypoxic tumor cells, but it is not catabolized. Aerobic tumor cells that are high lactate-consumers and likely express high MCT1/low <t>MCT4</t> can take up lactate and catabolize it to alanine (orange “ A ”) and glutamate (blue “ G ”), which will be exported from the cell. With the aerobic high lactate-consumer cell consuming lactate, more glucose can potentially be spared for hypoxic tumor cell use, potentially conferring a survival advantage. Aerobic tumor cells that are low lactate-consumers and likely express low MCT1/high MCT4 take up less lactate than the high lactate-consumers, consequently producing and exporting less alanine and glutamate. Low lactate-consumers utilize more glucose, which will not allow glucose to reach the hypoxic tumor cells ( A ). One proposed strategy for starving hypoxic tumor cells of glucose in a high lactate-consuming tumor is to treat with a MCT1- inhibitor, like CHC. CHC prevents lactate uptake and catabolism in cells, forcing the aerobic high lactate-consumer to use glucose, which starves the hypoxic tumor cell of glucose. Lactate transport out of hypoxic cells is also inhibited, which would also lead to hypoxic cell death ( B ).
    Mct4 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mct4 polyclonal antibody/product/Bioss
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    mct4 polyclonal antibody - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    Millipore mct4
    Summary diagram of lactate metabolism in high lactate-consumers vs. low lactate-consumers. The blue gradient represents oxygen diffusion. The cell on the left is hypoxic; the cells on the right are aerobic. Arrow colors correspond to substrates, and arrow size corresponds to relative amount. Hypoxic tumor cells take up glucose (gray “ Glc ”) and produce lactate (dark green “ L ”), leading to higher concentrations of lactate. Lactate may be taken up by the hypoxic tumor cells, but it is not catabolized. Aerobic tumor cells that are high lactate-consumers and likely express high MCT1/low <t>MCT4</t> can take up lactate and catabolize it to alanine (orange “ A ”) and glutamate (blue “ G ”), which will be exported from the cell. With the aerobic high lactate-consumer cell consuming lactate, more glucose can potentially be spared for hypoxic tumor cell use, potentially conferring a survival advantage. Aerobic tumor cells that are low lactate-consumers and likely express low MCT1/high MCT4 take up less lactate than the high lactate-consumers, consequently producing and exporting less alanine and glutamate. Low lactate-consumers utilize more glucose, which will not allow glucose to reach the hypoxic tumor cells ( A ). One proposed strategy for starving hypoxic tumor cells of glucose in a high lactate-consuming tumor is to treat with a MCT1- inhibitor, like CHC. CHC prevents lactate uptake and catabolism in cells, forcing the aerobic high lactate-consumer to use glucose, which starves the hypoxic tumor cell of glucose. Lactate transport out of hypoxic cells is also inhibited, which would also lead to hypoxic cell death ( B ).
    Mct4, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mct4/product/Millipore
    Average 92 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    mct4 - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Localisation of MCT1 and MCT4 in rat placenta at four different ages during gestation. The staining of rat placenta with antibodies to (a–d) MCT1 and (e–h) MCT4 is shown for (a, e) GD 11; (b, f) GD 14; (c, g) GD 18; and (d, h) GD 20. Maternal blood vessels and cells are indicated with stars (★) and foetal blood vessels and cells are indicated with arrow heads (▸). Original objective 40x, bar 15 μ m.

    Journal: International Journal of Cell Biology

    Article Title: Localisation of Lactate Transporters in Rat and Rabbit Placentae

    doi: 10.1155/2016/2084252

    Figure Lengend Snippet: Localisation of MCT1 and MCT4 in rat placenta at four different ages during gestation. The staining of rat placenta with antibodies to (a–d) MCT1 and (e–h) MCT4 is shown for (a, e) GD 11; (b, f) GD 14; (c, g) GD 18; and (d, h) GD 20. Maternal blood vessels and cells are indicated with stars (★) and foetal blood vessels and cells are indicated with arrow heads (▸). Original objective 40x, bar 15 μ m.

    Article Snippet: Slides were stained immunohistochemically using the Ventana Discovery XT slide staining system with rabbit anti-human MCT4 (Biorbyt LLC, San Francisco, CA; catalogue number orb137272) as the primary antibody, and goat anti-rabbit as the secondary antibody (Jackson Immunoresearch, reference number 111-066-003).

    Techniques: Staining

    Localisation of MCT1 and MCT4 in rabbit placenta at three different ages during gestation. The staining of rabbit placenta with antibodies to (a–c) MCT1 and (d–f) MCT4 is shown for (a, d) GD 13; (b, e) GD 18; and (c, f) GD 28. Maternal blood vessels and cells are indicated with stars (★) and foetal blood vessels and cells are indicated with arrow heads (▸). Original objective 40x, bar 15 μ m.

    Journal: International Journal of Cell Biology

    Article Title: Localisation of Lactate Transporters in Rat and Rabbit Placentae

    doi: 10.1155/2016/2084252

    Figure Lengend Snippet: Localisation of MCT1 and MCT4 in rabbit placenta at three different ages during gestation. The staining of rabbit placenta with antibodies to (a–c) MCT1 and (d–f) MCT4 is shown for (a, d) GD 13; (b, e) GD 18; and (c, f) GD 28. Maternal blood vessels and cells are indicated with stars (★) and foetal blood vessels and cells are indicated with arrow heads (▸). Original objective 40x, bar 15 μ m.

    Article Snippet: Slides were stained immunohistochemically using the Ventana Discovery XT slide staining system with rabbit anti-human MCT4 (Biorbyt LLC, San Francisco, CA; catalogue number orb137272) as the primary antibody, and goat anti-rabbit as the secondary antibody (Jackson Immunoresearch, reference number 111-066-003).

    Techniques: Staining

    sCPE regulates glucose- and lactate transporters, but does not affect gene expression of key metabolic enzymes (A) Immunoblot detection of GLUT1, GLUT3 and MCT4 in the lysates of the Neo or sCPE-transfected LNT229 or Tu140 cells upon CPE knockdown. For Tu140, control siRNA (si-mock) was used as negative control. α-tubulin was used as a loading control. The cells were serum-starved for 24h in serum-reduced medium prior to lysis. A representative immunoblot is shown. (B) Immunohistochemical staining of GLUT1 and LDHA in the Neo or sCPE-overexpressing LNT229 cells (20x magnification, scale bar 100μm). (C) Immunohistochemical staining of GLUT1 in the LNT229 spheres (20x magnification, scale bar 100μm). (D, E) qPCR analysis of CPE, Glut1, Glut3 and MCT4 gene expression in the (D) Neo or sCPE-overexpressing LNT229 cells or (E) Tu140 cells upon CPE knockdown. Control siRNA (si-mock) was used as negative control for CPE knockdown. Red dots represent single experiments. Unpaired t-test with Welch's correction. Mean±SEM; n=3 (D: *p=0.0105; E: *p=0.0422). (F, G) qPCR analysis of the (F) glycolytic enzymes (ALDOC, HKII, PFKP, PFKM, LDHA) and (G) enzymes involved in the pentose-phosphate pathway (G6PD, PGD, TALDO1, TKT, LDHB) in the Neo or sCPE-overexpressing LNT229 cells. Red dots represent single experiments. Unpaired t-test with Welch's correction. Mean±SEM; n=3.

    Journal: Oncotarget

    Article Title: Effects of soluble CPE on glioma cell migration are associated with mTOR activation and enhanced glucose flux

    doi: 10.18632/oncotarget.18747

    Figure Lengend Snippet: sCPE regulates glucose- and lactate transporters, but does not affect gene expression of key metabolic enzymes (A) Immunoblot detection of GLUT1, GLUT3 and MCT4 in the lysates of the Neo or sCPE-transfected LNT229 or Tu140 cells upon CPE knockdown. For Tu140, control siRNA (si-mock) was used as negative control. α-tubulin was used as a loading control. The cells were serum-starved for 24h in serum-reduced medium prior to lysis. A representative immunoblot is shown. (B) Immunohistochemical staining of GLUT1 and LDHA in the Neo or sCPE-overexpressing LNT229 cells (20x magnification, scale bar 100μm). (C) Immunohistochemical staining of GLUT1 in the LNT229 spheres (20x magnification, scale bar 100μm). (D, E) qPCR analysis of CPE, Glut1, Glut3 and MCT4 gene expression in the (D) Neo or sCPE-overexpressing LNT229 cells or (E) Tu140 cells upon CPE knockdown. Control siRNA (si-mock) was used as negative control for CPE knockdown. Red dots represent single experiments. Unpaired t-test with Welch's correction. Mean±SEM; n=3 (D: *p=0.0105; E: *p=0.0422). (F, G) qPCR analysis of the (F) glycolytic enzymes (ALDOC, HKII, PFKP, PFKM, LDHA) and (G) enzymes involved in the pentose-phosphate pathway (G6PD, PGD, TALDO1, TKT, LDHB) in the Neo or sCPE-overexpressing LNT229 cells. Red dots represent single experiments. Unpaired t-test with Welch's correction. Mean±SEM; n=3.

    Article Snippet: Following antibodies were used: mouse-anti-CPE (BD Bioscience, Franklin Lakes, NJ, USA), rabbit-anti-MCT4 (Novus Biologicals, Littleton, CO, USA), rabbit-anti-P-S6 ribosomal protein (S240/244) (D68F8) XP(R), rabbit-anti-P-S6 ribosomal protein (S235/236) (D57.2.2E) XP(R), mouse-anti-S6 ribosomal protein (54D2), rabbit-anti-P-4E-BP1 (T37/46) (236B4), rabbit-anti-4E-BP1 (53H11), rabbit-anti-P-AMPKα (T172) (40H9), rabbit-anti-P-Akt (S473), rabbit-anti-Akt, rabbit-anti-P-NDRG1 (T346) (D98G11) XP, rabbit-anti-NDRG1 (D8G9) XP(R) (all from Cell Signaling/New England Biolabs, Frankfurt a. M., Germany), mouse-anti-α-tubulin (Sigma Aldrich, Taufkirchen, Germany), rabbit-anti-GLUT3 (H50) (Santa Cruz, Heidelberg, Germany), rabbit-anti-β-actin and rabbit-anti-GLUT1 (Abcam, Cambridge, UK).

    Techniques: Expressing, Transfection, Negative Control, Lysis, Immunohistochemistry, Staining, Real-time Polymerase Chain Reaction

    Inhibition of CA9 and CA12 in rat NP cells has no effect on glycolytic flux A) Schematic of the glycolytic production of lactate/H + exported into the extracellular space via MCT4. B) Representative Western blot analysis of HIF-1α after inhibiting CA activity with MZA (500 nM), AZA (500 nM), and U-104 (2 μM) for 18 h in hypoxia. C) Densitometric analysis of Western blot experiment shown in (B) (n=4). D) HRE luciferase reporter activity was unaffected by CA inhibition (n=4, 3 technical replicates each). E) Representative Western blot analysis of MCT4 after inhibiting CA activity with MZA, AZA, and U-104. F) Densitometric analysis of Western blot experiment shown in (E) (n=4). G) Extracellular lactate concentrations were unaffected by CA inhibition with MZA, AZA and U-104 (n=5, 3 technical replicates each). Data is represented as mean ± SE, n≥ 4 independent biological replicates, * p

    Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

    Article Title: Bicarbonate Recycling by HIF-1-dependent Carbonic Anhydrase isoforms 9 and 12 is Critical in Maintaining Intracellular pH and Viability of Nucleus Pulposus Cells

    doi: 10.1002/jbmr.3293

    Figure Lengend Snippet: Inhibition of CA9 and CA12 in rat NP cells has no effect on glycolytic flux A) Schematic of the glycolytic production of lactate/H + exported into the extracellular space via MCT4. B) Representative Western blot analysis of HIF-1α after inhibiting CA activity with MZA (500 nM), AZA (500 nM), and U-104 (2 μM) for 18 h in hypoxia. C) Densitometric analysis of Western blot experiment shown in (B) (n=4). D) HRE luciferase reporter activity was unaffected by CA inhibition (n=4, 3 technical replicates each). E) Representative Western blot analysis of MCT4 after inhibiting CA activity with MZA, AZA, and U-104. F) Densitometric analysis of Western blot experiment shown in (E) (n=4). G) Extracellular lactate concentrations were unaffected by CA inhibition with MZA, AZA and U-104 (n=5, 3 technical replicates each). Data is represented as mean ± SE, n≥ 4 independent biological replicates, * p

    Article Snippet: Membranes were blocked with 5% nonfat dry milk in TBST (50 mM Tris pH 7.6, 150 mM NaCl, 0.1% Tween 20) and incubated overnight at 4°C in 5% nonfat dry milk in TBST with the anti-HIF-1α (1:500, R & D Systems); anti-CAIX (1:1000, Novus Biologicals), anti-CAXII (1:1000, Cell Signaling), anti-MCT4 (1:1000, Novus Biologicals), or anti-®-tubulin (1:5000, DSHB) antibodies.

    Techniques: Inhibition, Western Blot, Activity Assay, Luciferase

    Summary diagram of lactate metabolism in high lactate-consumers vs. low lactate-consumers. The blue gradient represents oxygen diffusion. The cell on the left is hypoxic; the cells on the right are aerobic. Arrow colors correspond to substrates, and arrow size corresponds to relative amount. Hypoxic tumor cells take up glucose (gray “ Glc ”) and produce lactate (dark green “ L ”), leading to higher concentrations of lactate. Lactate may be taken up by the hypoxic tumor cells, but it is not catabolized. Aerobic tumor cells that are high lactate-consumers and likely express high MCT1/low MCT4 can take up lactate and catabolize it to alanine (orange “ A ”) and glutamate (blue “ G ”), which will be exported from the cell. With the aerobic high lactate-consumer cell consuming lactate, more glucose can potentially be spared for hypoxic tumor cell use, potentially conferring a survival advantage. Aerobic tumor cells that are low lactate-consumers and likely express low MCT1/high MCT4 take up less lactate than the high lactate-consumers, consequently producing and exporting less alanine and glutamate. Low lactate-consumers utilize more glucose, which will not allow glucose to reach the hypoxic tumor cells ( A ). One proposed strategy for starving hypoxic tumor cells of glucose in a high lactate-consuming tumor is to treat with a MCT1- inhibitor, like CHC. CHC prevents lactate uptake and catabolism in cells, forcing the aerobic high lactate-consumer to use glucose, which starves the hypoxic tumor cell of glucose. Lactate transport out of hypoxic cells is also inhibited, which would also lead to hypoxic cell death ( B ).

    Journal: PLoS ONE

    Article Title: Catabolism of Exogenous Lactate Reveals It as a Legitimate Metabolic Substrate in Breast Cancer

    doi: 10.1371/journal.pone.0075154

    Figure Lengend Snippet: Summary diagram of lactate metabolism in high lactate-consumers vs. low lactate-consumers. The blue gradient represents oxygen diffusion. The cell on the left is hypoxic; the cells on the right are aerobic. Arrow colors correspond to substrates, and arrow size corresponds to relative amount. Hypoxic tumor cells take up glucose (gray “ Glc ”) and produce lactate (dark green “ L ”), leading to higher concentrations of lactate. Lactate may be taken up by the hypoxic tumor cells, but it is not catabolized. Aerobic tumor cells that are high lactate-consumers and likely express high MCT1/low MCT4 can take up lactate and catabolize it to alanine (orange “ A ”) and glutamate (blue “ G ”), which will be exported from the cell. With the aerobic high lactate-consumer cell consuming lactate, more glucose can potentially be spared for hypoxic tumor cell use, potentially conferring a survival advantage. Aerobic tumor cells that are low lactate-consumers and likely express low MCT1/high MCT4 take up less lactate than the high lactate-consumers, consequently producing and exporting less alanine and glutamate. Low lactate-consumers utilize more glucose, which will not allow glucose to reach the hypoxic tumor cells ( A ). One proposed strategy for starving hypoxic tumor cells of glucose in a high lactate-consuming tumor is to treat with a MCT1- inhibitor, like CHC. CHC prevents lactate uptake and catabolism in cells, forcing the aerobic high lactate-consumer to use glucose, which starves the hypoxic tumor cell of glucose. Lactate transport out of hypoxic cells is also inhibited, which would also lead to hypoxic cell death ( B ).

    Article Snippet: MCT1 rabbit anti-human IgG primary antibody (Millipore, Billerica, MA) and MCT4 rabbit (recognizes human, mouse and rat) IgG primary antibody (Santa Cruz Biotechnology, Dallas, TX, USA) were used, diluted 1∶1000 in TBST and incubated in 4°C overnight.

    Techniques: Diffusion-based Assay, Gas Chromatography