rabbit anti wnt1 polyclonal antibody Search Results


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    Abcam rabbit anti wnt1 antibody
    TTTY15 regulates the Wnt/β-catenin signaling pathway by adsorbing let-7a-5p (a) The online database TargetScan was used to predict the binding site between let-7a-5p and <t>Wnt1</t> mRNA 3'UTR. (b) WT Wnt1 and MUT Wnt1 luciferase reporters were co-transfected with let-7a-5p mimics (miR mimics) or let-7a-5p inhibitors (miR inhibitors) into NCI-N87 and SUN-1 cells, respectively, and dual-luciferase reporter gene assay was used to detect the luciferase activity. (c) Western blot was used to detect the regulatory effects of TTTY15 and let-7a-5p on the protein expression of Wnt1 and β-catenin in NCI-N87 and SUN-1 cells. (d) qRT-PCR was used to detect Wnt1 expression in 59 pairs of GC tissues and adjacent tissues. (e and f) Pearson correlation analysis of the correlation between the expressions of Wnt1 and let-7a-5p, and the expressions of Wnt1 and TTTY15 in GC tissues. All of the experiments were performed in triplicate. *** P < 0.001.
    Rabbit Anti Wnt1 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti wnt1 antibody/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti wnt1 antibody - by Bioz Stars, 2024-05
    86/100 stars
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    86
    Gentex Corporation rabbit anti wnt1 antibody
    TTTY15 regulates the Wnt/β-catenin signaling pathway by adsorbing let-7a-5p (a) The online database TargetScan was used to predict the binding site between let-7a-5p and <t>Wnt1</t> mRNA 3'UTR. (b) WT Wnt1 and MUT Wnt1 luciferase reporters were co-transfected with let-7a-5p mimics (miR mimics) or let-7a-5p inhibitors (miR inhibitors) into NCI-N87 and SUN-1 cells, respectively, and dual-luciferase reporter gene assay was used to detect the luciferase activity. (c) Western blot was used to detect the regulatory effects of TTTY15 and let-7a-5p on the protein expression of Wnt1 and β-catenin in NCI-N87 and SUN-1 cells. (d) qRT-PCR was used to detect Wnt1 expression in 59 pairs of GC tissues and adjacent tissues. (e and f) Pearson correlation analysis of the correlation between the expressions of Wnt1 and let-7a-5p, and the expressions of Wnt1 and TTTY15 in GC tissues. All of the experiments were performed in triplicate. *** P < 0.001.
    Rabbit Anti Wnt1 Antibody, supplied by Gentex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti wnt1 antibody/product/Gentex Corporation
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti wnt1 antibody - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology rabbit anti wnt1
    TTTY15 regulates the Wnt/β-catenin signaling pathway by adsorbing let-7a-5p (a) The online database TargetScan was used to predict the binding site between let-7a-5p and <t>Wnt1</t> mRNA 3'UTR. (b) WT Wnt1 and MUT Wnt1 luciferase reporters were co-transfected with let-7a-5p mimics (miR mimics) or let-7a-5p inhibitors (miR inhibitors) into NCI-N87 and SUN-1 cells, respectively, and dual-luciferase reporter gene assay was used to detect the luciferase activity. (c) Western blot was used to detect the regulatory effects of TTTY15 and let-7a-5p on the protein expression of Wnt1 and β-catenin in NCI-N87 and SUN-1 cells. (d) qRT-PCR was used to detect Wnt1 expression in 59 pairs of GC tissues and adjacent tissues. (e and f) Pearson correlation analysis of the correlation between the expressions of Wnt1 and let-7a-5p, and the expressions of Wnt1 and TTTY15 in GC tissues. All of the experiments were performed in triplicate. *** P < 0.001.
    Rabbit Anti Wnt1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti wnt1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti wnt1 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    Abcam anti rabbit wnt1
    TTTY15 regulates the Wnt/β-catenin signaling pathway by adsorbing let-7a-5p (a) The online database TargetScan was used to predict the binding site between let-7a-5p and <t>Wnt1</t> mRNA 3'UTR. (b) WT Wnt1 and MUT Wnt1 luciferase reporters were co-transfected with let-7a-5p mimics (miR mimics) or let-7a-5p inhibitors (miR inhibitors) into NCI-N87 and SUN-1 cells, respectively, and dual-luciferase reporter gene assay was used to detect the luciferase activity. (c) Western blot was used to detect the regulatory effects of TTTY15 and let-7a-5p on the protein expression of Wnt1 and β-catenin in NCI-N87 and SUN-1 cells. (d) qRT-PCR was used to detect Wnt1 expression in 59 pairs of GC tissues and adjacent tissues. (e and f) Pearson correlation analysis of the correlation between the expressions of Wnt1 and let-7a-5p, and the expressions of Wnt1 and TTTY15 in GC tissues. All of the experiments were performed in triplicate. *** P < 0.001.
    Anti Rabbit Wnt1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit wnt1/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rabbit wnt1 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    Abcam rabbit anti wnt1
    Lin28E1 is a 698 bp region within the second intron, which was identified as a Lin28a enhancer. ( b ) Expression pattern of a Lin28E1 driven eGFP construct, which is active in the ectoderm (Ect) and at the neural plate border (NPB). Immunohistochemistry for Lin28a ( c ) and <t>Wnt1</t> ( d ) on transverse sections of HH9 embryo (arrows point to the dorsal tube). ( e–f ) Comparison of Lin28E1 (left) and MUT Lin28E1 (right) reporter activity in a bilaterally electroporated embryo. Dorsal view of the head of a whole mount embryo ( e ) and transverse section ( f , arrows point to neural crest cells). ( g ) Western blot for flag-Lef1 following enhancer pull down experiment with wild type and mutant Lin28E1 . ( h ) RT-PCR for endogenous Lin28a mRNA, Lin28E1 reporter and mature let-7 miRNAs on combinatorial knockdown of Wnt1 and Wnt4 ( i ) Chromatin immunoprecipitation for Lef-1 and β-catenin (Ctnnb1), performed with neural folds of WT embryos, and embryos electroporated with Wnt dominant negative construct. ( j ) Diagram outlining the parameters for single cell measurements of let-7 sensor fluorescence and nuclear β-catenin (Ctnnb1), as a function of the distance to the dorsal neural tube. Fluorescence intensity of nuclear β-catenin ( k ) and let-7 sensor ( l ) is inversely correlated with the distance of the neural crest cell from the neural tube. In both graphs, each dot represents a single cell. ( m ) Quantitative comparison of transcript levels of Axin2 , Lin28a, let-7 sensor, Pax7 and FoxD3 in control vs. Wnt1 overexpressing neural crest cells ( n ) Model summarizing the results. AU: arbitrary units, DN: dominant negative, DNT: dorsal neural tube, IGG: Immunoglobulin G, Lin28E1 : Lin28a Enhancer 1, Neg region: negative control region. Error bars in ( h ) and ( m ) represent standard error and error bars in ( i ) reflect standard deviation between technical replicates. 10.7554/eLife.40556.018 Figure 5—source data 1. Data for the RT-PCR experiements shown in and quantification of single cell measurements of let-7 -sensor activity and nuclear b-catenin.
    Rabbit Anti Wnt1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti wnt1/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti wnt1 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    TTTY15 regulates the Wnt/β-catenin signaling pathway by adsorbing let-7a-5p (a) The online database TargetScan was used to predict the binding site between let-7a-5p and Wnt1 mRNA 3'UTR. (b) WT Wnt1 and MUT Wnt1 luciferase reporters were co-transfected with let-7a-5p mimics (miR mimics) or let-7a-5p inhibitors (miR inhibitors) into NCI-N87 and SUN-1 cells, respectively, and dual-luciferase reporter gene assay was used to detect the luciferase activity. (c) Western blot was used to detect the regulatory effects of TTTY15 and let-7a-5p on the protein expression of Wnt1 and β-catenin in NCI-N87 and SUN-1 cells. (d) qRT-PCR was used to detect Wnt1 expression in 59 pairs of GC tissues and adjacent tissues. (e and f) Pearson correlation analysis of the correlation between the expressions of Wnt1 and let-7a-5p, and the expressions of Wnt1 and TTTY15 in GC tissues. All of the experiments were performed in triplicate. *** P < 0.001.

    Journal: Bioengineered

    Article Title: Male-specific long non-coding RNA testis-specific transcript, Y-linked 15 promotes gastric cancer cell growth by regulating Wnt family member 1/β-catenin signaling by sponging microRNA let-7a-5p

    doi: 10.1080/21655979.2022.2053814

    Figure Lengend Snippet: TTTY15 regulates the Wnt/β-catenin signaling pathway by adsorbing let-7a-5p (a) The online database TargetScan was used to predict the binding site between let-7a-5p and Wnt1 mRNA 3'UTR. (b) WT Wnt1 and MUT Wnt1 luciferase reporters were co-transfected with let-7a-5p mimics (miR mimics) or let-7a-5p inhibitors (miR inhibitors) into NCI-N87 and SUN-1 cells, respectively, and dual-luciferase reporter gene assay was used to detect the luciferase activity. (c) Western blot was used to detect the regulatory effects of TTTY15 and let-7a-5p on the protein expression of Wnt1 and β-catenin in NCI-N87 and SUN-1 cells. (d) qRT-PCR was used to detect Wnt1 expression in 59 pairs of GC tissues and adjacent tissues. (e and f) Pearson correlation analysis of the correlation between the expressions of Wnt1 and let-7a-5p, and the expressions of Wnt1 and TTTY15 in GC tissues. All of the experiments were performed in triplicate. *** P < 0.001.

    Article Snippet: Then, the membranes were washed with tris buffered saline tween (TBST) solution, and they were incubated overnight with rabbit anti-N-cadherin antibody (Abcam, ab76011, 1:500), rabbit anti-E-cadherin antibody (Abcam, ab212059, 1:500), rabbit anti-Wnt1 antibody (Abcam, ab15251, 1:500), rabbit anti-β-catenin (Abcam, ab32572, 1:500), rabbit anti-β-actin antibody (Abcam, ab8227, 1:500) at 4°C.

    Techniques: Binding Assay, Luciferase, Transfection, Reporter Gene Assay, Activity Assay, Western Blot, Expressing, Quantitative RT-PCR

    Lin28E1 is a 698 bp region within the second intron, which was identified as a Lin28a enhancer. ( b ) Expression pattern of a Lin28E1 driven eGFP construct, which is active in the ectoderm (Ect) and at the neural plate border (NPB). Immunohistochemistry for Lin28a ( c ) and Wnt1 ( d ) on transverse sections of HH9 embryo (arrows point to the dorsal tube). ( e–f ) Comparison of Lin28E1 (left) and MUT Lin28E1 (right) reporter activity in a bilaterally electroporated embryo. Dorsal view of the head of a whole mount embryo ( e ) and transverse section ( f , arrows point to neural crest cells). ( g ) Western blot for flag-Lef1 following enhancer pull down experiment with wild type and mutant Lin28E1 . ( h ) RT-PCR for endogenous Lin28a mRNA, Lin28E1 reporter and mature let-7 miRNAs on combinatorial knockdown of Wnt1 and Wnt4 ( i ) Chromatin immunoprecipitation for Lef-1 and β-catenin (Ctnnb1), performed with neural folds of WT embryos, and embryos electroporated with Wnt dominant negative construct. ( j ) Diagram outlining the parameters for single cell measurements of let-7 sensor fluorescence and nuclear β-catenin (Ctnnb1), as a function of the distance to the dorsal neural tube. Fluorescence intensity of nuclear β-catenin ( k ) and let-7 sensor ( l ) is inversely correlated with the distance of the neural crest cell from the neural tube. In both graphs, each dot represents a single cell. ( m ) Quantitative comparison of transcript levels of Axin2 , Lin28a, let-7 sensor, Pax7 and FoxD3 in control vs. Wnt1 overexpressing neural crest cells ( n ) Model summarizing the results. AU: arbitrary units, DN: dominant negative, DNT: dorsal neural tube, IGG: Immunoglobulin G, Lin28E1 : Lin28a Enhancer 1, Neg region: negative control region. Error bars in ( h ) and ( m ) represent standard error and error bars in ( i ) reflect standard deviation between technical replicates. 10.7554/eLife.40556.018 Figure 5—source data 1. Data for the RT-PCR experiements shown in and quantification of single cell measurements of let-7 -sensor activity and nuclear b-catenin.

    Journal: eLife

    Article Title: Control of neural crest multipotency by Wnt signaling and the Lin28/ let-7 axis

    doi: 10.7554/eLife.40556

    Figure Lengend Snippet: Lin28E1 is a 698 bp region within the second intron, which was identified as a Lin28a enhancer. ( b ) Expression pattern of a Lin28E1 driven eGFP construct, which is active in the ectoderm (Ect) and at the neural plate border (NPB). Immunohistochemistry for Lin28a ( c ) and Wnt1 ( d ) on transverse sections of HH9 embryo (arrows point to the dorsal tube). ( e–f ) Comparison of Lin28E1 (left) and MUT Lin28E1 (right) reporter activity in a bilaterally electroporated embryo. Dorsal view of the head of a whole mount embryo ( e ) and transverse section ( f , arrows point to neural crest cells). ( g ) Western blot for flag-Lef1 following enhancer pull down experiment with wild type and mutant Lin28E1 . ( h ) RT-PCR for endogenous Lin28a mRNA, Lin28E1 reporter and mature let-7 miRNAs on combinatorial knockdown of Wnt1 and Wnt4 ( i ) Chromatin immunoprecipitation for Lef-1 and β-catenin (Ctnnb1), performed with neural folds of WT embryos, and embryos electroporated with Wnt dominant negative construct. ( j ) Diagram outlining the parameters for single cell measurements of let-7 sensor fluorescence and nuclear β-catenin (Ctnnb1), as a function of the distance to the dorsal neural tube. Fluorescence intensity of nuclear β-catenin ( k ) and let-7 sensor ( l ) is inversely correlated with the distance of the neural crest cell from the neural tube. In both graphs, each dot represents a single cell. ( m ) Quantitative comparison of transcript levels of Axin2 , Lin28a, let-7 sensor, Pax7 and FoxD3 in control vs. Wnt1 overexpressing neural crest cells ( n ) Model summarizing the results. AU: arbitrary units, DN: dominant negative, DNT: dorsal neural tube, IGG: Immunoglobulin G, Lin28E1 : Lin28a Enhancer 1, Neg region: negative control region. Error bars in ( h ) and ( m ) represent standard error and error bars in ( i ) reflect standard deviation between technical replicates. 10.7554/eLife.40556.018 Figure 5—source data 1. Data for the RT-PCR experiements shown in and quantification of single cell measurements of let-7 -sensor activity and nuclear b-catenin.

    Article Snippet: Antibody , Rabbit Anti-Wnt1 , Abcam Cat #: ab15251 , RRID: AB_301792 , IHC 1:200.

    Techniques: Expressing, Construct, Immunohistochemistry, Activity Assay, Western Blot, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Chromatin Immunoprecipitation, Dominant Negative Mutation, Fluorescence, Negative Control, Standard Deviation

    ( b ) RT-PCR comparing the levels of Runx2 , Barx2 and Alx1 in control vs. Wnt1 overexpressing neural crest cells sorted from HH12 embryo. Error bars represent standard error.

    Journal: eLife

    Article Title: Control of neural crest multipotency by Wnt signaling and the Lin28/ let-7 axis

    doi: 10.7554/eLife.40556

    Figure Lengend Snippet: ( b ) RT-PCR comparing the levels of Runx2 , Barx2 and Alx1 in control vs. Wnt1 overexpressing neural crest cells sorted from HH12 embryo. Error bars represent standard error.

    Article Snippet: Antibody , Rabbit Anti-Wnt1 , Abcam Cat #: ab15251 , RRID: AB_301792 , IHC 1:200.

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Journal: eLife

    Article Title: Control of neural crest multipotency by Wnt signaling and the Lin28/ let-7 axis

    doi: 10.7554/eLife.40556

    Figure Lengend Snippet:

    Article Snippet: Antibody , Rabbit Anti-Wnt1 , Abcam Cat #: ab15251 , RRID: AB_301792 , IHC 1:200.

    Techniques: Recombinant, Plasmid Preparation, Sequencing, SYBR Green Assay, Software