Journal: British Journal of Cancer

Article Title: Cell line and patient-derived xenograft models reveal elevated CDCP1 as a target in high-grade serous ovarian cancer

doi: 10.1038/bjc.2015.471

Figure Lengend Snippet: Silencing of CDCP1 reduces intraperitoneal tumour formation of SKOV3 cells in mice. Female NSG mice were injected with SKOV3-shScramble ( n =5) or SKOV3-shCDCP1 ( n =5) cells (5 × 10 6 ). ( A ) Left, Bioluminescent images of mice after 5 weeks of tumour growth. Right, Graph of the bioluminescent signal (total flux; photons per seconds) obtained from each mouse. ( B ) Left, Representative images of the peritoneal cavity of mice at the time of killing at 5 weeks after injection of SKOV3-shScramble or SKOV3-shCDCP1 cells. Arrows indicate tumour nodules. Right, Graph of the number of peritoneal tumour nodules present in mice injected with either SKOV3-shScramble or SKOV3-shCDCP1 cells. Data represent mean and standard deviation of each group. ( C ) Western blot analysis of lysates from three randomly selected SKOV3-shScramble and SKOV3-shCDCP1 xenograft tumours recovered from mice for CDCP1, p-Src-Y416 (pSrc), Src and GAPDH.

Article Snippet: Whole-cell lysates were collected using RIPA buffer (Sigma-Aldrich) with 1 × Complete protease inhibitor cocktail (Roche, Castle Hill, NSW, Australia), 2 mM sodium vanadate, and 10 mM sodium fluoride, and used in western blot analysis as described previously ( He et al , 2015 ) using mouse monoclonal anti-p53 antibody DO-7 (Sigma-Aldrich), rabbit anti-CDCP1 carboxyl terminal antibody #4115, rabbit anti-phospho-Src-Tyr416 (p-Src-Y416) antibody #2101, mouse anti-Src antibody #2110 (Cell Signaling Technology) or a mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Merck Millipore, Kilsyth, VIC, Australia).

Techniques: Injection, Standard Deviation, Western Blot

Journal: British Journal of Cancer

Article Title: Cell line and patient-derived xenograft models reveal elevated CDCP1 as a target in high-grade serous ovarian cancer

doi: 10.1038/bjc.2015.471

Figure Lengend Snippet: Monoclonal antibody targeting of CDCP1 impedes HGSC PDX growth. ( A ) H&E and anti-CDCP1 (brown) staining of three patient tumours and PDXs developed from these tumours. Magnification, × 40. CDCP1 is expressed by malignant cells, detected mainly in the membrane with cytosolic staining also apparent. Scale bar is 50 μ m. ( B – D ) Mice were injected with cell slurry from the PDX of patient 28 along with antibody 10D7 or isotype matched control IgG (100 μ g). 10D7 and IgG treatments continued weekly at 25 mg kg −1 per mouse ( n =4 mice per treatment). ( B ) Representative images, at the time of killing of mice at week 7, of the peritoneal cavity of mice treated with IgG (left) or 10D7 (right). ( C ) Graphical representation of the total number of tumours (left), the weight of the largest tumour (middle) and the weight of all tumours combined (right) of groups of mice treated with IgG or 10D7. Data represent mean and standard deviation of each group. ( D ) Western blot analysis of lysates from four randomly selected IgG- and 10D7-treated PDX tumours recovered from mice. Lysates were examined for CDCP1, p-Src-Y416 (pSrc), Src and GAPDH.

Article Snippet: Whole-cell lysates were collected using RIPA buffer (Sigma-Aldrich) with 1 × Complete protease inhibitor cocktail (Roche, Castle Hill, NSW, Australia), 2 mM sodium vanadate, and 10 mM sodium fluoride, and used in western blot analysis as described previously ( He et al , 2015 ) using mouse monoclonal anti-p53 antibody DO-7 (Sigma-Aldrich), rabbit anti-CDCP1 carboxyl terminal antibody #4115, rabbit anti-phospho-Src-Tyr416 (p-Src-Y416) antibody #2101, mouse anti-Src antibody #2110 (Cell Signaling Technology) or a mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Merck Millipore, Kilsyth, VIC, Australia).

Techniques: Staining, Injection, Standard Deviation, Western Blot

Journal: FEBS Open Bio

Article Title: The EGF / EGFR axis and its downstream signaling pathways regulate the motility and proliferation of cultured oral keratinocytes

doi: 10.1002/2211-5463.13653

Figure Lengend Snippet: List of reagents and antibodies used in this study.

Article Snippet: Phospho‐Src Family (Tyr416) (D49G4) Rabbit mAb , Cell Signaling Technology , #6943 , 1 : 1000.

Techniques: Concentration Assay