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    Cell Signaling Technology Inc rabbit perk1 2
    FGF-dependent elevation of the distal neural folds forms Closure 5. (A) Reflection confocal images of the PNP (asterisk and black arrowheads) of a CS11 human embryo and closed neural tube of a CS13 human embryo. Dashed magenta lines indicate the borders of the neuroepithelium. Scale bars: 50 µm. (B) Representative confocal images of phalloidin-stained mouse PNPs at sequential stages of closure (E9.5-E10.5), illustrating the formation of Closure 5 and completion of closure. Scale bar: 100 µm. (C) Confocal image of a phalloidin-stained PNP from a 20-somite stage mouse embryo showing optical cross-sections at the level of the zippering point and at 90% of the length from the proximal end of the PNP. White arrow indicates zippering point elevation; red arrow indicates eversion of the caudal neural folds relative to the apical surface of the neuroepithelium. (D) Equivalent to C showing a 27-somite stage mouse embryo PNP indicating elevation of the caudal neural folds. (E) Quantification of elevation at the zippering point and caudal neural folds (90% of the length of the PNP) in mouse embryos at the indicated somite stages. (F) Whole-mount confocal image of a 29 somite-stage mouse PNP stained for <t>pERK1/2</t> immunolocalization. The two images were acquired using different objectives. Scale bars: 100 µm. (G) Confocal images of a vehicle-treated and two pan-FGFR inhibitor-treated mouse embryos after 24 h of whole-embryo culture. Dashed red lines indicate the abnormal neuroepithelium that protrudes out of the PNP in inhibitor-treated embryos. Scale bar: 100 µm. Magenta asterisks indicate the rostral zippering point; cyan asterisks indicate Closure 5.
    Rabbit Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit perk1 2/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    rabbit perk1 2 - by Bioz Stars, 2024-07
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    86
    Cell Signaling Technology Inc rabbit anti perk1 2
    KEY RESOURCES TABLE
    Rabbit Anti Perk1 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti perk1 2/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti perk1 2 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    FGF-dependent elevation of the distal neural folds forms Closure 5. (A) Reflection confocal images of the PNP (asterisk and black arrowheads) of a CS11 human embryo and closed neural tube of a CS13 human embryo. Dashed magenta lines indicate the borders of the neuroepithelium. Scale bars: 50 µm. (B) Representative confocal images of phalloidin-stained mouse PNPs at sequential stages of closure (E9.5-E10.5), illustrating the formation of Closure 5 and completion of closure. Scale bar: 100 µm. (C) Confocal image of a phalloidin-stained PNP from a 20-somite stage mouse embryo showing optical cross-sections at the level of the zippering point and at 90% of the length from the proximal end of the PNP. White arrow indicates zippering point elevation; red arrow indicates eversion of the caudal neural folds relative to the apical surface of the neuroepithelium. (D) Equivalent to C showing a 27-somite stage mouse embryo PNP indicating elevation of the caudal neural folds. (E) Quantification of elevation at the zippering point and caudal neural folds (90% of the length of the PNP) in mouse embryos at the indicated somite stages. (F) Whole-mount confocal image of a 29 somite-stage mouse PNP stained for pERK1/2 immunolocalization. The two images were acquired using different objectives. Scale bars: 100 µm. (G) Confocal images of a vehicle-treated and two pan-FGFR inhibitor-treated mouse embryos after 24 h of whole-embryo culture. Dashed red lines indicate the abnormal neuroepithelium that protrudes out of the PNP in inhibitor-treated embryos. Scale bar: 100 µm. Magenta asterisks indicate the rostral zippering point; cyan asterisks indicate Closure 5.

    Journal: Development (Cambridge, England)

    Article Title: Caudal Fgfr1 disruption produces localised spinal mis-patterning and a terminal myelocystocele-like phenotype in mice

    doi: 10.1242/dev.202139

    Figure Lengend Snippet: FGF-dependent elevation of the distal neural folds forms Closure 5. (A) Reflection confocal images of the PNP (asterisk and black arrowheads) of a CS11 human embryo and closed neural tube of a CS13 human embryo. Dashed magenta lines indicate the borders of the neuroepithelium. Scale bars: 50 µm. (B) Representative confocal images of phalloidin-stained mouse PNPs at sequential stages of closure (E9.5-E10.5), illustrating the formation of Closure 5 and completion of closure. Scale bar: 100 µm. (C) Confocal image of a phalloidin-stained PNP from a 20-somite stage mouse embryo showing optical cross-sections at the level of the zippering point and at 90% of the length from the proximal end of the PNP. White arrow indicates zippering point elevation; red arrow indicates eversion of the caudal neural folds relative to the apical surface of the neuroepithelium. (D) Equivalent to C showing a 27-somite stage mouse embryo PNP indicating elevation of the caudal neural folds. (E) Quantification of elevation at the zippering point and caudal neural folds (90% of the length of the PNP) in mouse embryos at the indicated somite stages. (F) Whole-mount confocal image of a 29 somite-stage mouse PNP stained for pERK1/2 immunolocalization. The two images were acquired using different objectives. Scale bars: 100 µm. (G) Confocal images of a vehicle-treated and two pan-FGFR inhibitor-treated mouse embryos after 24 h of whole-embryo culture. Dashed red lines indicate the abnormal neuroepithelium that protrudes out of the PNP in inhibitor-treated embryos. Scale bar: 100 µm. Magenta asterisks indicate the rostral zippering point; cyan asterisks indicate Closure 5.

    Article Snippet: Primary antibodies were used at 1:100 dilution and were as follows: rabbit E-cadherin (3195, Cell Signaling Technology), mouse N-cadherin (14215S, Cell Signaling Technology), rabbit pERK1/2 (9101, Cell Signalling Technology), mouse FGFR1 (ab824, Abcam) mouse anti-RALDH2 (sc-166362, Santa Cruz) and rabbit anti pFGFR1 (Tyr 653/654, GTX133526, Stratech).

    Techniques: Staining, Embryo Culture

    FGF signalling is required for timely PNP closure. (A) Whole-mount stained confocal images showing pERK1/2 immunolocalization in vehicle- and pan-FGFRi-treated embryos after 24 h of whole-embryo culture. pERK-bright cells persist around the PNP rim in the pan-FGFRi-treated embryo. Scale bar: 100 µm. (B) Whole-mount Fgf8 in situ hybridisation in vehicle- and pan-FGFRi-treated embryos after 8 h of whole-embryo culture. Dashed white lines indicate the end of the tailbud; arrows indicate the forelimb buds. Scale bar: 500 µm. (C) Bright-field images showing RARE-mediated LacZ expression domain in vehicle and pan-FGFRi-treated embryos after 24 h of whole-embryo culture. An equivalently processed negative (−ve) control embryo lacking LacZ is also shown. The white bracket indicates the distance between the RARE domain and the zippering point. The black bracket indicates that the RARE domain does not extend to the end of the PNP in pan-FGFRi-treated embryos. Scale bar: 350 µm. (D) Quantification of RARE domain distance to the end of zippering point in vehicle and pan-FGFRi-treated embryos after 24 h of whole-embryo culture. Individual points represent data from individual embryos; P -value was calculated using an unpaired t -test. (E) Whole-mount immunolocalization of Fgfr1 in a 28-somite stage embryo. Scale bar: 100 µm. (F) Confocal images of embryos treated with vehicle or a second Fgfr1-targeting antagonist after 24 h of whole-embryo culture. Dashed red lines outline the abnormal neuroepithelium in inhibitor-treated embryos. Scale bar: 100 µm. (G) Quantification of the proportion of embryos with closed PNPs, open PNPs with Closure 5 morphology (caudally narrowed PNP with elevated caudal neural folds that meet dorsally) or open PNPs without Closure 5 in vehicle and FGFR1-targeting inhibitor-treated embryos after 24 h of whole-embryo culture. Numbers indicate the number of embryos observed in each category. Magenta asterisks indicate the rostral zippering point; cyan asterisks indicate Closure 5.

    Journal: Development (Cambridge, England)

    Article Title: Caudal Fgfr1 disruption produces localised spinal mis-patterning and a terminal myelocystocele-like phenotype in mice

    doi: 10.1242/dev.202139

    Figure Lengend Snippet: FGF signalling is required for timely PNP closure. (A) Whole-mount stained confocal images showing pERK1/2 immunolocalization in vehicle- and pan-FGFRi-treated embryos after 24 h of whole-embryo culture. pERK-bright cells persist around the PNP rim in the pan-FGFRi-treated embryo. Scale bar: 100 µm. (B) Whole-mount Fgf8 in situ hybridisation in vehicle- and pan-FGFRi-treated embryos after 8 h of whole-embryo culture. Dashed white lines indicate the end of the tailbud; arrows indicate the forelimb buds. Scale bar: 500 µm. (C) Bright-field images showing RARE-mediated LacZ expression domain in vehicle and pan-FGFRi-treated embryos after 24 h of whole-embryo culture. An equivalently processed negative (−ve) control embryo lacking LacZ is also shown. The white bracket indicates the distance between the RARE domain and the zippering point. The black bracket indicates that the RARE domain does not extend to the end of the PNP in pan-FGFRi-treated embryos. Scale bar: 350 µm. (D) Quantification of RARE domain distance to the end of zippering point in vehicle and pan-FGFRi-treated embryos after 24 h of whole-embryo culture. Individual points represent data from individual embryos; P -value was calculated using an unpaired t -test. (E) Whole-mount immunolocalization of Fgfr1 in a 28-somite stage embryo. Scale bar: 100 µm. (F) Confocal images of embryos treated with vehicle or a second Fgfr1-targeting antagonist after 24 h of whole-embryo culture. Dashed red lines outline the abnormal neuroepithelium in inhibitor-treated embryos. Scale bar: 100 µm. (G) Quantification of the proportion of embryos with closed PNPs, open PNPs with Closure 5 morphology (caudally narrowed PNP with elevated caudal neural folds that meet dorsally) or open PNPs without Closure 5 in vehicle and FGFR1-targeting inhibitor-treated embryos after 24 h of whole-embryo culture. Numbers indicate the number of embryos observed in each category. Magenta asterisks indicate the rostral zippering point; cyan asterisks indicate Closure 5.

    Article Snippet: Primary antibodies were used at 1:100 dilution and were as follows: rabbit E-cadherin (3195, Cell Signaling Technology), mouse N-cadherin (14215S, Cell Signaling Technology), rabbit pERK1/2 (9101, Cell Signalling Technology), mouse FGFR1 (ab824, Abcam) mouse anti-RALDH2 (sc-166362, Santa Cruz) and rabbit anti pFGFR1 (Tyr 653/654, GTX133526, Stratech).

    Techniques: Staining, Embryo Culture, In Situ, Hybridization, Expressing

    KEY RESOURCES TABLE

    Journal: Developmental cell

    Article Title: VEGF receptor 2 (KDR) protects airways from mucus metaplasia through a Sox9 dependent pathway

    doi: 10.1016/j.devcel.2021.04.027

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Primary antibodies used for immunostaining analysis include rabbit anti-p63 (1:200, sc-8343, Santa Cruz Biotechnology); mouse anti-p63 (1:500, CM163, Biocare); chicken anti-GFP (1:1000, GFP-1020, Aves Labs); rabbit anti-Sox9 (1:1000, AB5535, Millipore); goat anti-tdTomato (1:1000, orb182397, Biorbyt); mouse anti-Foxj1 (1:100, 14–9965-82, eBioscience); rabbit anti-pMek1/2 (1:200, 2338S, Cell Signaling Technology); rabbit anti-pErk1/2 (1:200, 4370S, Cell Signaling Technology); rabbit anti-pP38 (1:200, 9215S, Cell Signaling Technology); rabbit anti-pJNK (1:200, 9251S, Cell Signaling Technology); rabbit anti-Kdr (1:200, 9698S, Cell Signaling Technology); mouse anti-Muc5AC (1:500, MS-145-P0, Lab Vision); rabbit anti-Clca3 (1:2000, ab46512, Abcam); rabbit anti-Scgb1a1 (1:500, 07–623, Millipore); rat anti-human SCGB1A1 (1:1000, MAB4218, R&D Systems); mouse anti-acetylated-tubulin (1:2000, T7451, Sigma).

    Techniques: Recombinant, Staining, RNA Sequencing Assay, Software