rabbit anti kv7 2 antibody Search Results


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  • 93
    Alomone Labs rabbit anti kv7 2 antibody
    Inhibition of AMPK activity restored acute stress-induced decrease in membrane fraction of Kv7.3 subunit A and B: Gel images ( A ) and quantification of band density normalized by GAPDH ( B ) show the total protein levels of AMPK in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) of GAPDH-normalized band density of membrane fraction of <t>Kv7.2</t> and Kv7.3 in the PVN in stressed rats treated with ICV injection of vehicle and dorsomorphin (100 nmol in 10 µl aCSF). E and F , Original gel images ( E ) and quantification ( F ) of GAPDH-normalized band density of cytosolic fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with vehicle and dorsomorphin. The molecular weight was indicated on the right side of the gel images. In all the protein assays, 4 samples (each sample contains PVN tissues of 3 rats) were used in each group. *p
    Rabbit Anti Kv7 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti kv7 2 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti kv7 2 antibody - by Bioz Stars, 2022-10
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    91
    Abcam anti kcnq2 antibody
    Inhibition of AMPK activity restored acute stress-induced decrease in membrane fraction of Kv7.3 subunit A and B: Gel images ( A ) and quantification of band density normalized by GAPDH ( B ) show the total protein levels of AMPK in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) of GAPDH-normalized band density of membrane fraction of <t>Kv7.2</t> and Kv7.3 in the PVN in stressed rats treated with ICV injection of vehicle and dorsomorphin (100 nmol in 10 µl aCSF). E and F , Original gel images ( E ) and quantification ( F ) of GAPDH-normalized band density of cytosolic fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with vehicle and dorsomorphin. The molecular weight was indicated on the right side of the gel images. In all the protein assays, 4 samples (each sample contains PVN tissues of 3 rats) were used in each group. *p
    Anti Kcnq2 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kcnq2 antibody/product/Abcam
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kcnq2 antibody - by Bioz Stars, 2022-10
    91/100 stars
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    93
    Alomone Labs anti kcnq2 antibody
    Inhibition of AMPK activity restored acute stress-induced decrease in membrane fraction of Kv7.3 subunit A and B: Gel images ( A ) and quantification of band density normalized by GAPDH ( B ) show the total protein levels of AMPK in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) of GAPDH-normalized band density of membrane fraction of <t>Kv7.2</t> and Kv7.3 in the PVN in stressed rats treated with ICV injection of vehicle and dorsomorphin (100 nmol in 10 µl aCSF). E and F , Original gel images ( E ) and quantification ( F ) of GAPDH-normalized band density of cytosolic fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with vehicle and dorsomorphin. The molecular weight was indicated on the right side of the gel images. In all the protein assays, 4 samples (each sample contains PVN tissues of 3 rats) were used in each group. *p
    Anti Kcnq2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kcnq2 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kcnq2 antibody - by Bioz Stars, 2022-10
    93/100 stars
      Buy from Supplier

    93
    Alomone Labs rabbit anti kv7 2
    NP1 interacts and colocalizes with <t>Kv7.2</t> at presynaptic terminals of excitatory synapses and axonal growth cones. A – D , Representative Western blots of immunoprecipitation eluates separated in 6% Tris-Glycine (for high molecular weight proteins) and 4–12% Bis-Tris gels (for low molecular weight proteins). TL, Total protein lysate; Syx, syntaxin. A , Both native Kv7.2 and syntaxin 1A, but not Kv7.3, coprecipitate with NP1 in total brain extracts. B , Both native Kv7.2 and NP1 coprecipitate with syntaxin in total brain extracts. C , D , Recombinant NP1 coprecipitates Kv7.2, but not syntaxin or Kv7.3, in 293T cells transfected with NP1, 5Myc-Kv7.2, 2HA-Kv7.3, and syntaxin 1A cDNAs. Kv7.2 and Kv7.3 were immunoprecipitated with antibodies against their respective Myc and HA tags. E , F , Immunofluorescence studies and confocal microscopy were performed in high-density ( E ) or low-density ( F ) isolated cortical neurons. E , Top, Confocal sections of 0.772 μm in the z -plane showing immunofluorescence of NP1, VGLUT1, Kv7.2, and negative control (omitting primary antibodies). Bottom, Colocalization (in white) of the excitatory presynaptic marker VGLUT1 (blue) with NP1 (green) and Kv7.2 (red) is shown in a single section with the corresponding orthogonal views of the stack of confocal sections. White arrows indicate sites of colocalization. F , NP1 (green) and KV7.2 (magenta) immunofluorescence and DIC images of an isolated cortical cultured neuron (1×) with its corresponding axonal growth cone highlighted in a white square box, shown in a confocal section of 0.772 μm in the z -plane at higher (5×) magnification. The negative control for primary antibodies is shown in another growth cone on the right. The image in the bottom is the merge of NP1 and Kv7.2 immunofluorescence images in the single confocal section obtained at 5× showing colocalization (white) of NP1 and Kv7.2 in the growth cone, with the corresponding orthogonal views from its respective stack of confocal sections. Images were acquired using restricted spectral emission wavelength ranges chosen to avoid crosstalk or bleed-through between the three different channels. Scale bar, 5 μm.
    Rabbit Anti Kv7 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti kv7 2/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti kv7 2 - by Bioz Stars, 2022-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of AMPK activity restored acute stress-induced decrease in membrane fraction of Kv7.3 subunit A and B: Gel images ( A ) and quantification of band density normalized by GAPDH ( B ) show the total protein levels of AMPK in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) of GAPDH-normalized band density of membrane fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with ICV injection of vehicle and dorsomorphin (100 nmol in 10 µl aCSF). E and F , Original gel images ( E ) and quantification ( F ) of GAPDH-normalized band density of cytosolic fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with vehicle and dorsomorphin. The molecular weight was indicated on the right side of the gel images. In all the protein assays, 4 samples (each sample contains PVN tissues of 3 rats) were used in each group. *p

    Journal: Neuropharmacology

    Article Title: Acute Stress Diminishes M-current Contributing to Elevated Activity of Hypothalamic-Pituitary-Adrenal Axis

    doi: 10.1016/j.neuropharm.2016.11.024

    Figure Lengend Snippet: Inhibition of AMPK activity restored acute stress-induced decrease in membrane fraction of Kv7.3 subunit A and B: Gel images ( A ) and quantification of band density normalized by GAPDH ( B ) show the total protein levels of AMPK in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) of GAPDH-normalized band density of membrane fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with ICV injection of vehicle and dorsomorphin (100 nmol in 10 µl aCSF). E and F , Original gel images ( E ) and quantification ( F ) of GAPDH-normalized band density of cytosolic fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with vehicle and dorsomorphin. The molecular weight was indicated on the right side of the gel images. In all the protein assays, 4 samples (each sample contains PVN tissues of 3 rats) were used in each group. *p

    Article Snippet: The immunoblots were probed with a rabbit anti-Kv7.2 antibody (1:1000, Alomone Labs) and rabbit anti-Kv7.3 antibody (1:1000, Alomone Labs).

    Techniques: Inhibition, Activity Assay, Injection, Molecular Weight

    Acute stress diminished excitatory effect of M-current blocker and reduced M-current in PVN-CRH neurons ( A ): Confocal images show the distribution of Kv7.2 or Kv7.3 in the PVN-CRH neurons. B and C : Original recordings ( B ) and summary data ( C ) show the firing activity of eGFP-tagged PVN neurons in basal condition, the presence of Kv7 channel blocker XE-991 (3 µM), and washout in unstressed and acutely stressed rats (n = 20 neurons in unstressed and stressed rats, respectively). ( D) : Acute stress significantly reduced the increase in firing activity induced by XE-991. ( E) : Representative traces show deactivation tail currents in the absence (basal) or presence of Kv7 opener retigabine (10 µM) and blocker XE-991 (3 µM) in eGFP-tagged PVN neurons in stressed and unstressed rats. The deactivation tail currents were elicited by the voltage protocol shown in the insert. ( F ): summary data shows the basal and retigabine-induced M-currents, which were defined as XE-991-sensitive tail currents in basal and the presence of retigabine, were significantly diminished in the PVN-CRH neurons in stressed rats (n =15 neurons in 4 rats) compared with unstressed rats (n = 12 neurons in 4 rats).* p

    Journal: Neuropharmacology

    Article Title: Acute Stress Diminishes M-current Contributing to Elevated Activity of Hypothalamic-Pituitary-Adrenal Axis

    doi: 10.1016/j.neuropharm.2016.11.024

    Figure Lengend Snippet: Acute stress diminished excitatory effect of M-current blocker and reduced M-current in PVN-CRH neurons ( A ): Confocal images show the distribution of Kv7.2 or Kv7.3 in the PVN-CRH neurons. B and C : Original recordings ( B ) and summary data ( C ) show the firing activity of eGFP-tagged PVN neurons in basal condition, the presence of Kv7 channel blocker XE-991 (3 µM), and washout in unstressed and acutely stressed rats (n = 20 neurons in unstressed and stressed rats, respectively). ( D) : Acute stress significantly reduced the increase in firing activity induced by XE-991. ( E) : Representative traces show deactivation tail currents in the absence (basal) or presence of Kv7 opener retigabine (10 µM) and blocker XE-991 (3 µM) in eGFP-tagged PVN neurons in stressed and unstressed rats. The deactivation tail currents were elicited by the voltage protocol shown in the insert. ( F ): summary data shows the basal and retigabine-induced M-currents, which were defined as XE-991-sensitive tail currents in basal and the presence of retigabine, were significantly diminished in the PVN-CRH neurons in stressed rats (n =15 neurons in 4 rats) compared with unstressed rats (n = 12 neurons in 4 rats).* p

    Article Snippet: The immunoblots were probed with a rabbit anti-Kv7.2 antibody (1:1000, Alomone Labs) and rabbit anti-Kv7.3 antibody (1:1000, Alomone Labs).

    Techniques: Activity Assay

    Kv7.2 and Kv7.3 protein expression levels in the PVN in stressed and unstressed rats A and B , Representative blots ( A ) and quantification ( B ) show the total protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) show the membrane fraction protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats. E and F , Original gel images ( E ) and quantification ( F ) show the cytosolic fraction protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats (n = 4 samples, each sample contains PVN tissues of 3 rats) were used in each group. * p

    Journal: Neuropharmacology

    Article Title: Acute Stress Diminishes M-current Contributing to Elevated Activity of Hypothalamic-Pituitary-Adrenal Axis

    doi: 10.1016/j.neuropharm.2016.11.024

    Figure Lengend Snippet: Kv7.2 and Kv7.3 protein expression levels in the PVN in stressed and unstressed rats A and B , Representative blots ( A ) and quantification ( B ) show the total protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) show the membrane fraction protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats. E and F , Original gel images ( E ) and quantification ( F ) show the cytosolic fraction protein levels of Kv7.2 and Kv7.3 in the PVN in unstressed and stressed rats (n = 4 samples, each sample contains PVN tissues of 3 rats) were used in each group. * p

    Article Snippet: The immunoblots were probed with a rabbit anti-Kv7.2 antibody (1:1000, Alomone Labs) and rabbit anti-Kv7.3 antibody (1:1000, Alomone Labs).

    Techniques: Expressing

    NP1 interacts and colocalizes with Kv7.2 at presynaptic terminals of excitatory synapses and axonal growth cones. A – D , Representative Western blots of immunoprecipitation eluates separated in 6% Tris-Glycine (for high molecular weight proteins) and 4–12% Bis-Tris gels (for low molecular weight proteins). TL, Total protein lysate; Syx, syntaxin. A , Both native Kv7.2 and syntaxin 1A, but not Kv7.3, coprecipitate with NP1 in total brain extracts. B , Both native Kv7.2 and NP1 coprecipitate with syntaxin in total brain extracts. C , D , Recombinant NP1 coprecipitates Kv7.2, but not syntaxin or Kv7.3, in 293T cells transfected with NP1, 5Myc-Kv7.2, 2HA-Kv7.3, and syntaxin 1A cDNAs. Kv7.2 and Kv7.3 were immunoprecipitated with antibodies against their respective Myc and HA tags. E , F , Immunofluorescence studies and confocal microscopy were performed in high-density ( E ) or low-density ( F ) isolated cortical neurons. E , Top, Confocal sections of 0.772 μm in the z -plane showing immunofluorescence of NP1, VGLUT1, Kv7.2, and negative control (omitting primary antibodies). Bottom, Colocalization (in white) of the excitatory presynaptic marker VGLUT1 (blue) with NP1 (green) and Kv7.2 (red) is shown in a single section with the corresponding orthogonal views of the stack of confocal sections. White arrows indicate sites of colocalization. F , NP1 (green) and KV7.2 (magenta) immunofluorescence and DIC images of an isolated cortical cultured neuron (1×) with its corresponding axonal growth cone highlighted in a white square box, shown in a confocal section of 0.772 μm in the z -plane at higher (5×) magnification. The negative control for primary antibodies is shown in another growth cone on the right. The image in the bottom is the merge of NP1 and Kv7.2 immunofluorescence images in the single confocal section obtained at 5× showing colocalization (white) of NP1 and Kv7.2 in the growth cone, with the corresponding orthogonal views from its respective stack of confocal sections. Images were acquired using restricted spectral emission wavelength ranges chosen to avoid crosstalk or bleed-through between the three different channels. Scale bar, 5 μm.

    Journal: The Journal of Neuroscience

    Article Title: Neuronal Pentraxin 1 Negatively Regulates Excitatory Synapse Density and Synaptic Plasticity

    doi: 10.1523/JNEUROSCI.2548-14.2015

    Figure Lengend Snippet: NP1 interacts and colocalizes with Kv7.2 at presynaptic terminals of excitatory synapses and axonal growth cones. A – D , Representative Western blots of immunoprecipitation eluates separated in 6% Tris-Glycine (for high molecular weight proteins) and 4–12% Bis-Tris gels (for low molecular weight proteins). TL, Total protein lysate; Syx, syntaxin. A , Both native Kv7.2 and syntaxin 1A, but not Kv7.3, coprecipitate with NP1 in total brain extracts. B , Both native Kv7.2 and NP1 coprecipitate with syntaxin in total brain extracts. C , D , Recombinant NP1 coprecipitates Kv7.2, but not syntaxin or Kv7.3, in 293T cells transfected with NP1, 5Myc-Kv7.2, 2HA-Kv7.3, and syntaxin 1A cDNAs. Kv7.2 and Kv7.3 were immunoprecipitated with antibodies against their respective Myc and HA tags. E , F , Immunofluorescence studies and confocal microscopy were performed in high-density ( E ) or low-density ( F ) isolated cortical neurons. E , Top, Confocal sections of 0.772 μm in the z -plane showing immunofluorescence of NP1, VGLUT1, Kv7.2, and negative control (omitting primary antibodies). Bottom, Colocalization (in white) of the excitatory presynaptic marker VGLUT1 (blue) with NP1 (green) and Kv7.2 (red) is shown in a single section with the corresponding orthogonal views of the stack of confocal sections. White arrows indicate sites of colocalization. F , NP1 (green) and KV7.2 (magenta) immunofluorescence and DIC images of an isolated cortical cultured neuron (1×) with its corresponding axonal growth cone highlighted in a white square box, shown in a confocal section of 0.772 μm in the z -plane at higher (5×) magnification. The negative control for primary antibodies is shown in another growth cone on the right. The image in the bottom is the merge of NP1 and Kv7.2 immunofluorescence images in the single confocal section obtained at 5× showing colocalization (white) of NP1 and Kv7.2 in the growth cone, with the corresponding orthogonal views from its respective stack of confocal sections. Images were acquired using restricted spectral emission wavelength ranges chosen to avoid crosstalk or bleed-through between the three different channels. Scale bar, 5 μm.

    Article Snippet: We used the following antibodies: mouse anti-NP1 antibody (1:1000; Becton Dickinson), mouse anti-synaptophysin, clone SY38 (1:1000), mouse anti-PSD95 (1:2000; both from Millipore Bioscience Research Reagents), rabbit anti-Kv7.2 (1:200; Alomone Labs), mouse anti-syntaxin 1A (HPC-1 clone, 1:2000; Sigma-Aldrich), mouse anti-HA (1:3000, Covance), and mouse anti-Myc (9E10 clone, 1:5000; Sigma-Aldrich).

    Techniques: Western Blot, Immunoprecipitation, Molecular Weight, Recombinant, Transfection, Immunofluorescence, Confocal Microscopy, Isolation, Negative Control, Marker, Cell Culture

    Knockdown of NP1 reduces Kv7.2 surface expression and M-current. A , B , The proportion of Kv7.2 at the neuronal surface was determined by biotinylation (30 min at 4°C) of all surface proteins in cultured cortical neurons. The ratio of the surface over the total amount of receptor was determined by densitometric analysis and expressed as a percentage of control values. Only samples of biotinylated proteins lacking immunoreactivity to actin, which indicates contamination with intracellular proteins, were used for analyses. A , Representative Western blots of Kv7.2, Na-KATPase, and actin from total (T) and biotinylated (B) proteins. B , Quantitative analysis of the effect of gain and loss of NP1 on the surface/total levels of the Kv7.2 subunit of the voltage-gated Kv7/M K + channels. Values are mean ± SEM of shRdm ( n = 11), shNP1 ( n = 11), and pWPI-NP1 ( n = 5), from at least three independent experiments; * p

    Journal: The Journal of Neuroscience

    Article Title: Neuronal Pentraxin 1 Negatively Regulates Excitatory Synapse Density and Synaptic Plasticity

    doi: 10.1523/JNEUROSCI.2548-14.2015

    Figure Lengend Snippet: Knockdown of NP1 reduces Kv7.2 surface expression and M-current. A , B , The proportion of Kv7.2 at the neuronal surface was determined by biotinylation (30 min at 4°C) of all surface proteins in cultured cortical neurons. The ratio of the surface over the total amount of receptor was determined by densitometric analysis and expressed as a percentage of control values. Only samples of biotinylated proteins lacking immunoreactivity to actin, which indicates contamination with intracellular proteins, were used for analyses. A , Representative Western blots of Kv7.2, Na-KATPase, and actin from total (T) and biotinylated (B) proteins. B , Quantitative analysis of the effect of gain and loss of NP1 on the surface/total levels of the Kv7.2 subunit of the voltage-gated Kv7/M K + channels. Values are mean ± SEM of shRdm ( n = 11), shNP1 ( n = 11), and pWPI-NP1 ( n = 5), from at least three independent experiments; * p

    Article Snippet: We used the following antibodies: mouse anti-NP1 antibody (1:1000; Becton Dickinson), mouse anti-synaptophysin, clone SY38 (1:1000), mouse anti-PSD95 (1:2000; both from Millipore Bioscience Research Reagents), rabbit anti-Kv7.2 (1:200; Alomone Labs), mouse anti-syntaxin 1A (HPC-1 clone, 1:2000; Sigma-Aldrich), mouse anti-HA (1:3000, Covance), and mouse anti-Myc (9E10 clone, 1:5000; Sigma-Aldrich).

    Techniques: Expressing, Cell Culture, Western Blot