rabbit anti fos primary antibody Search Results


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  • 86
    Bio-Rad anti c fos
    Anti C Fos, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti c fos/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti c fos - by Bioz Stars, 2024-03
    86/100 stars
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    86
    Millipore rabbit anti fos antibody
    PKC-dependent effects on <t>FOS</t> and CYP17 require ERK1/2 activation. (A,B) H295R cells were incubated for the indicated times with TPA (10 nM; A) or with Ang II (100 nM; B). Real-time RT-PCR analysis was used to quantify FOS transcript levels. (C,D) Cells were incubated for 5 minutes with TPA (10 nM; C) or with Ang II (100 nM; D) alone or in the presence of PD98059 (PD; 10 μM) added 30 minutes before stimulation. Phosphorylation of ERK1/2 was examined by immunoblot analysis using equivalent amounts of proteins. ERK2 was used as a loading control. (E) Cells were incubated for 1 hour with TPA (10 nM) alone or in the presence of PD (10 μM) or U0126 (10 μM) added 30 minutes before stimulation. Real-time RT-PCR analysis was used to quantify FOS transcript levels. (F) Cells were incubated for 24 hours with TPA (10 nM) alone or in the presence of PD (10 μM) or U0126 (10 μM) added 30 minutes before stimulation. Real-time RT-PCR analysis was used to quantify CYP17 transcript levels. Real-time RT-PCR data represent the mean + s.e.m. of three independent RNA samples from H295R cultures and are expressed as relative difference from basal (calibrator). *P<0.001 compared with TPA. (G) Cells were incubated for 1 hour with TPA (10 nM) and Ang II (100 nM) alone or in the presence of PD (10 μM) added 30 minutes before stimulation. Phosphorylation of FOS on Ser375 and Thr325 and of ERK1/2 were examined by immunoblot analysis using equivalent amounts of proteins. GAPDH was used as a loading control.
    Rabbit Anti Fos Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti fos antibody/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti fos antibody - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

    86
    Synaptic Systems rabbit anti fos antibody
    PKC-dependent effects on <t>FOS</t> and CYP17 require ERK1/2 activation. (A,B) H295R cells were incubated for the indicated times with TPA (10 nM; A) or with Ang II (100 nM; B). Real-time RT-PCR analysis was used to quantify FOS transcript levels. (C,D) Cells were incubated for 5 minutes with TPA (10 nM; C) or with Ang II (100 nM; D) alone or in the presence of PD98059 (PD; 10 μM) added 30 minutes before stimulation. Phosphorylation of ERK1/2 was examined by immunoblot analysis using equivalent amounts of proteins. ERK2 was used as a loading control. (E) Cells were incubated for 1 hour with TPA (10 nM) alone or in the presence of PD (10 μM) or U0126 (10 μM) added 30 minutes before stimulation. Real-time RT-PCR analysis was used to quantify FOS transcript levels. (F) Cells were incubated for 24 hours with TPA (10 nM) alone or in the presence of PD (10 μM) or U0126 (10 μM) added 30 minutes before stimulation. Real-time RT-PCR analysis was used to quantify CYP17 transcript levels. Real-time RT-PCR data represent the mean + s.e.m. of three independent RNA samples from H295R cultures and are expressed as relative difference from basal (calibrator). *P<0.001 compared with TPA. (G) Cells were incubated for 1 hour with TPA (10 nM) and Ang II (100 nM) alone or in the presence of PD (10 μM) added 30 minutes before stimulation. Phosphorylation of FOS on Ser375 and Thr325 and of ERK1/2 were examined by immunoblot analysis using equivalent amounts of proteins. GAPDH was used as a loading control.
    Rabbit Anti Fos Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti fos antibody/product/Synaptic Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti fos antibody - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology rabbit anti fos antibody
    PKC-dependent effects on <t>FOS</t> and CYP17 require ERK1/2 activation. (A,B) H295R cells were incubated for the indicated times with TPA (10 nM; A) or with Ang II (100 nM; B). Real-time RT-PCR analysis was used to quantify FOS transcript levels. (C,D) Cells were incubated for 5 minutes with TPA (10 nM; C) or with Ang II (100 nM; D) alone or in the presence of PD98059 (PD; 10 μM) added 30 minutes before stimulation. Phosphorylation of ERK1/2 was examined by immunoblot analysis using equivalent amounts of proteins. ERK2 was used as a loading control. (E) Cells were incubated for 1 hour with TPA (10 nM) alone or in the presence of PD (10 μM) or U0126 (10 μM) added 30 minutes before stimulation. Real-time RT-PCR analysis was used to quantify FOS transcript levels. (F) Cells were incubated for 24 hours with TPA (10 nM) alone or in the presence of PD (10 μM) or U0126 (10 μM) added 30 minutes before stimulation. Real-time RT-PCR analysis was used to quantify CYP17 transcript levels. Real-time RT-PCR data represent the mean + s.e.m. of three independent RNA samples from H295R cultures and are expressed as relative difference from basal (calibrator). *P<0.001 compared with TPA. (G) Cells were incubated for 1 hour with TPA (10 nM) and Ang II (100 nM) alone or in the presence of PD (10 μM) added 30 minutes before stimulation. Phosphorylation of FOS on Ser375 and Thr325 and of ERK1/2 were examined by immunoblot analysis using equivalent amounts of proteins. GAPDH was used as a loading control.
    Rabbit Anti Fos Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti fos antibody/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti fos antibody - by Bioz Stars, 2024-03
    86/100 stars
      Buy from Supplier

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    PKC-dependent effects on FOS and CYP17 require ERK1/2 activation. (A,B) H295R cells were incubated for the indicated times with TPA (10 nM; A) or with Ang II (100 nM; B). Real-time RT-PCR analysis was used to quantify FOS transcript levels. (C,D) Cells were incubated for 5 minutes with TPA (10 nM; C) or with Ang II (100 nM; D) alone or in the presence of PD98059 (PD; 10 μM) added 30 minutes before stimulation. Phosphorylation of ERK1/2 was examined by immunoblot analysis using equivalent amounts of proteins. ERK2 was used as a loading control. (E) Cells were incubated for 1 hour with TPA (10 nM) alone or in the presence of PD (10 μM) or U0126 (10 μM) added 30 minutes before stimulation. Real-time RT-PCR analysis was used to quantify FOS transcript levels. (F) Cells were incubated for 24 hours with TPA (10 nM) alone or in the presence of PD (10 μM) or U0126 (10 μM) added 30 minutes before stimulation. Real-time RT-PCR analysis was used to quantify CYP17 transcript levels. Real-time RT-PCR data represent the mean + s.e.m. of three independent RNA samples from H295R cultures and are expressed as relative difference from basal (calibrator). *P<0.001 compared with TPA. (G) Cells were incubated for 1 hour with TPA (10 nM) and Ang II (100 nM) alone or in the presence of PD (10 μM) added 30 minutes before stimulation. Phosphorylation of FOS on Ser375 and Thr325 and of ERK1/2 were examined by immunoblot analysis using equivalent amounts of proteins. GAPDH was used as a loading control.

    Journal: Journal of Cell Science

    Article Title: The AP-1 family member FOS blocks transcriptional activity of the nuclear receptor steroidogenic factor 1

    doi: 10.1242/jcs.055806

    Figure Lengend Snippet: PKC-dependent effects on FOS and CYP17 require ERK1/2 activation. (A,B) H295R cells were incubated for the indicated times with TPA (10 nM; A) or with Ang II (100 nM; B). Real-time RT-PCR analysis was used to quantify FOS transcript levels. (C,D) Cells were incubated for 5 minutes with TPA (10 nM; C) or with Ang II (100 nM; D) alone or in the presence of PD98059 (PD; 10 μM) added 30 minutes before stimulation. Phosphorylation of ERK1/2 was examined by immunoblot analysis using equivalent amounts of proteins. ERK2 was used as a loading control. (E) Cells were incubated for 1 hour with TPA (10 nM) alone or in the presence of PD (10 μM) or U0126 (10 μM) added 30 minutes before stimulation. Real-time RT-PCR analysis was used to quantify FOS transcript levels. (F) Cells were incubated for 24 hours with TPA (10 nM) alone or in the presence of PD (10 μM) or U0126 (10 μM) added 30 minutes before stimulation. Real-time RT-PCR analysis was used to quantify CYP17 transcript levels. Real-time RT-PCR data represent the mean + s.e.m. of three independent RNA samples from H295R cultures and are expressed as relative difference from basal (calibrator). *P<0.001 compared with TPA. (G) Cells were incubated for 1 hour with TPA (10 nM) and Ang II (100 nM) alone or in the presence of PD (10 μM) added 30 minutes before stimulation. Phosphorylation of FOS on Ser375 and Thr325 and of ERK1/2 were examined by immunoblot analysis using equivalent amounts of proteins. GAPDH was used as a loading control.

    Article Snippet: For immunohistochemistry, tissues were blocked with normal goat serum followed by incubation overnight with rabbit anti-CYP17 antibody (1/2000) and rabbit anti-FOS antibody (Calbiochem, 1/500).

    Techniques: Activation Assay, Incubation, Quantitative RT-PCR, Western Blot

    An inverse correlation exists between FOS and CYP17 expression in human adrenal. (A) Immunohistochemical analysis of FOS (left upper photo) and CYP17 (right upper photo) in human adult adrenal glands. Double immunostaining for FOS and CYP17 (left lower photo). The right lower panel is the negative control for FOS expression. Immunoreactivity is indicated with brown staining; Hematoxylin was used for counterstaining, which gave the blue coloring. For double immunostaining for FOS and CYP17, the first antibody used was FOS and it was visualized by DAB (brown). Then a CYP17 antibody was used, which was stained by alkaline phosphatase substrate kit III (blue). Each panel is of a serial section of the same adrenal gland. G, glomerulosa; F, fasciculata. Scale bars: 100 μm. (B) Double immunofluorescence for SF-1 and c-FOS in the human adrenal gland. The majority of adrenocortical cells in the zona glomerulosa were positive for both SF-1 and c-FOS. SF-1 immunoreactivity was represented as red (Alexa Fluor 647), whereas c-FOS immunoreactivity was shown as green (Alexa Fluor 488). Colocalization of SF-1 and c-FOS are indicated as yellow color in the merged photo. Nuclei of H295R cells were visualized by DAPI staining. Scale bars: 100 μm. (C,D) H295R cells were grown for 2 days in the presence of siRNA for FOS and then treated for 1 hour (C) or 24 hours (D) with TPA (10 nM). Real-time PCR for FOS (C) and CYP17 (D) in H295R cells following siRNA gene silencing was performed. Data represent the mean + s.e.m. of two independent RNA samples from H295R cultures and are expressed as relative difference from cells untreated and transfected with scrambled siRNA (basal calibrator).

    Journal: Journal of Cell Science

    Article Title: The AP-1 family member FOS blocks transcriptional activity of the nuclear receptor steroidogenic factor 1

    doi: 10.1242/jcs.055806

    Figure Lengend Snippet: An inverse correlation exists between FOS and CYP17 expression in human adrenal. (A) Immunohistochemical analysis of FOS (left upper photo) and CYP17 (right upper photo) in human adult adrenal glands. Double immunostaining for FOS and CYP17 (left lower photo). The right lower panel is the negative control for FOS expression. Immunoreactivity is indicated with brown staining; Hematoxylin was used for counterstaining, which gave the blue coloring. For double immunostaining for FOS and CYP17, the first antibody used was FOS and it was visualized by DAB (brown). Then a CYP17 antibody was used, which was stained by alkaline phosphatase substrate kit III (blue). Each panel is of a serial section of the same adrenal gland. G, glomerulosa; F, fasciculata. Scale bars: 100 μm. (B) Double immunofluorescence for SF-1 and c-FOS in the human adrenal gland. The majority of adrenocortical cells in the zona glomerulosa were positive for both SF-1 and c-FOS. SF-1 immunoreactivity was represented as red (Alexa Fluor 647), whereas c-FOS immunoreactivity was shown as green (Alexa Fluor 488). Colocalization of SF-1 and c-FOS are indicated as yellow color in the merged photo. Nuclei of H295R cells were visualized by DAPI staining. Scale bars: 100 μm. (C,D) H295R cells were grown for 2 days in the presence of siRNA for FOS and then treated for 1 hour (C) or 24 hours (D) with TPA (10 nM). Real-time PCR for FOS (C) and CYP17 (D) in H295R cells following siRNA gene silencing was performed. Data represent the mean + s.e.m. of two independent RNA samples from H295R cultures and are expressed as relative difference from cells untreated and transfected with scrambled siRNA (basal calibrator).

    Article Snippet: For immunohistochemistry, tissues were blocked with normal goat serum followed by incubation overnight with rabbit anti-CYP17 antibody (1/2000) and rabbit anti-FOS antibody (Calbiochem, 1/500).

    Techniques: Expressing, Immunohistochemical staining, Double Immunostaining, Negative Control, Staining, Immunofluorescence, Real-time Polymerase Chain Reaction, Transfection

    JUN and FOS proteins are recruited to the CYP17 promoter and repress its transcription. (A,B) H295R cells were untreated (basal) or incubated for 1 hour with Ang II (100 nM) or TPA (10 nM) alone or in the presence of PD98059 (10 μM). In vivo binding of JUN and FOS to the CYP17 promoter was examined using ChIP assay. Immunoprecipitated (JUN, FOS) and total (10% input) DNA were subject to real time RT-PCR using specific primers. Ct values from immunoprecipitated samples were normalized to the input Ct values (*P<0.001 compared with basal and **P<0.001 compared with TPA). (C) H295R cells were transfected with a CYP17 promoter construct (1 μg) and 0.1 μg of expression vector for JUN and FOS. (D) H295R cells were co-transfected with 1 μg of a luciferase promoter construct containing 381 bp of the CYP17 promoter, increasing doses of FOS (0.3, 1, 3, 10, 30, 100 ng), SF-1 (0.3 μg) and JUN (0.1 μg) expression plasmids. (E,F) H295R cells were co-transfected with 1 μg of CYP17 reporter construct mutated at the SF-1 sites (CYP17 SF-1/3mut; E) or of CYP17 AP1 −164 mut (F) together with SF-1 (0.3 μg), JUN (0.1 μg) and FOS (0.1 μg). (G) Cells were transfected with CYP17 reporter construct (WT) or a construct containing 381 bp of the promoter region mutated at an AP-1 site (CYP17 AP1 −164 mut). (H) Cells were transfected with the CYP17 reporter and increasing doses of JUN. Data were normalized to co-expressed β-gal. Results represent the mean + s.e.m. of data from two to three independent experiments, each performed in triplicate. *P<0.001 compared with SF-1; ^P<0.001 compared with basal (without expression vectors) mut CYP17; **P<0.05 compared with CYP17 WT; +P<0.001 compared with basal (without expression vectors) WT CYP17.

    Journal: Journal of Cell Science

    Article Title: The AP-1 family member FOS blocks transcriptional activity of the nuclear receptor steroidogenic factor 1

    doi: 10.1242/jcs.055806

    Figure Lengend Snippet: JUN and FOS proteins are recruited to the CYP17 promoter and repress its transcription. (A,B) H295R cells were untreated (basal) or incubated for 1 hour with Ang II (100 nM) or TPA (10 nM) alone or in the presence of PD98059 (10 μM). In vivo binding of JUN and FOS to the CYP17 promoter was examined using ChIP assay. Immunoprecipitated (JUN, FOS) and total (10% input) DNA were subject to real time RT-PCR using specific primers. Ct values from immunoprecipitated samples were normalized to the input Ct values (*P<0.001 compared with basal and **P<0.001 compared with TPA). (C) H295R cells were transfected with a CYP17 promoter construct (1 μg) and 0.1 μg of expression vector for JUN and FOS. (D) H295R cells were co-transfected with 1 μg of a luciferase promoter construct containing 381 bp of the CYP17 promoter, increasing doses of FOS (0.3, 1, 3, 10, 30, 100 ng), SF-1 (0.3 μg) and JUN (0.1 μg) expression plasmids. (E,F) H295R cells were co-transfected with 1 μg of CYP17 reporter construct mutated at the SF-1 sites (CYP17 SF-1/3mut; E) or of CYP17 AP1 −164 mut (F) together with SF-1 (0.3 μg), JUN (0.1 μg) and FOS (0.1 μg). (G) Cells were transfected with CYP17 reporter construct (WT) or a construct containing 381 bp of the promoter region mutated at an AP-1 site (CYP17 AP1 −164 mut). (H) Cells were transfected with the CYP17 reporter and increasing doses of JUN. Data were normalized to co-expressed β-gal. Results represent the mean + s.e.m. of data from two to three independent experiments, each performed in triplicate. *P<0.001 compared with SF-1; ^P<0.001 compared with basal (without expression vectors) mut CYP17; **P<0.05 compared with CYP17 WT; +P<0.001 compared with basal (without expression vectors) WT CYP17.

    Article Snippet: For immunohistochemistry, tissues were blocked with normal goat serum followed by incubation overnight with rabbit anti-CYP17 antibody (1/2000) and rabbit anti-FOS antibody (Calbiochem, 1/500).

    Techniques: Incubation, In Vivo, Binding Assay, Immunoprecipitation, Quantitative RT-PCR, Transfection, Construct, Expressing, Plasmid Preparation, Luciferase

    Direct interaction between SF-1 and FOS. (A) H295R cells were transfected with the CYP17 reporter plasmid and increasing doses of SF-1 expression vector and 0.1 μg of JUN and FOS expression vectors. (B) H295R cells were transfected with a luciferase promoter construct containing AP-1 sites. Cells were cotransfected with increasing doses of SF-1 expression vector and 0.1 μg of expression vector for JUN and FOS. (C,D) H295R cells were treated for the indicated times with Ang II (100 nM; C) or TPA (10 nM; D). Nuclear extracts were immunoprecipitated (IP) with an anti-SF-1 antibody and then used for immunoblot analysis (WB) using an anti-FOS monoclonal antibody. (E) Schematic diagram depicting deletion fragments of the SF-1 coding region. DNA fragments were cloned into pGEX4T1 and used in GST pulldown experiments. (F,G) One hundred micrograms of purified GST-tagged SF-1 proteins were incubated with 5 μl of in-vitro-synthesized FOS (F) or SRC1 (G) protein. Immunocomplexes (IP) were formed using an anti-SF-1 antibody. Precipitated samples were used for western blot analysis using an anti-FOS monoclonal antibody or an anti-SRC-1 antibody. The GST protein was immunoprecipitated like the samples and used as negative control (NC). One microliter of in-vitro-synthesized FOS or SRC-1 was loaded on the gel and used as positive control (input). GST pulldown results shown represent data from three independent experiments.

    Journal: Journal of Cell Science

    Article Title: The AP-1 family member FOS blocks transcriptional activity of the nuclear receptor steroidogenic factor 1

    doi: 10.1242/jcs.055806

    Figure Lengend Snippet: Direct interaction between SF-1 and FOS. (A) H295R cells were transfected with the CYP17 reporter plasmid and increasing doses of SF-1 expression vector and 0.1 μg of JUN and FOS expression vectors. (B) H295R cells were transfected with a luciferase promoter construct containing AP-1 sites. Cells were cotransfected with increasing doses of SF-1 expression vector and 0.1 μg of expression vector for JUN and FOS. (C,D) H295R cells were treated for the indicated times with Ang II (100 nM; C) or TPA (10 nM; D). Nuclear extracts were immunoprecipitated (IP) with an anti-SF-1 antibody and then used for immunoblot analysis (WB) using an anti-FOS monoclonal antibody. (E) Schematic diagram depicting deletion fragments of the SF-1 coding region. DNA fragments were cloned into pGEX4T1 and used in GST pulldown experiments. (F,G) One hundred micrograms of purified GST-tagged SF-1 proteins were incubated with 5 μl of in-vitro-synthesized FOS (F) or SRC1 (G) protein. Immunocomplexes (IP) were formed using an anti-SF-1 antibody. Precipitated samples were used for western blot analysis using an anti-FOS monoclonal antibody or an anti-SRC-1 antibody. The GST protein was immunoprecipitated like the samples and used as negative control (NC). One microliter of in-vitro-synthesized FOS or SRC-1 was loaded on the gel and used as positive control (input). GST pulldown results shown represent data from three independent experiments.

    Article Snippet: For immunohistochemistry, tissues were blocked with normal goat serum followed by incubation overnight with rabbit anti-CYP17 antibody (1/2000) and rabbit anti-FOS antibody (Calbiochem, 1/500).

    Techniques: Transfection, Plasmid Preparation, Expressing, Luciferase, Construct, Immunoprecipitation, Western Blot, Clone Assay, Purification, Incubation, In Vitro, Synthesized, Negative Control, Positive Control

    FOS competes with co-factors binding to SF-1 on the CYP17 promoter. (A–C) In vivo binding of SF-1 (A) to the CYP17 promoter was examined using a ChIP assay. Interaction of SRC1 (B) and CBP (C) to SF-1 on the CYP17 promoter was tested with a re-ChIP assay. All samples were immunoprecipitated with an anti-SF-1 antibody. For re-ChIP, samples were re-precipitated with an anti-SRC1 or anti-CBP antibody. Immunoprecipitated (SF-1, SF-1/SRC1, SF-1/CBP) and total (10% input) DNA were subject to real-time RT-PCR using specific primers. Ct values from immunoprecipitated samples were normalized to the input Ct values. Results represent the mean + s.e.m. of data from 3 independent experiments (*P<0.001 compared with basal and **P<0.001 compared with TPA). (D) H295R cells were transfected with the luciferase promoter construct containing 381 bp of the promoter region of the human CYP17 gene in the presence of the expression vector for SF-1 (0.3 μg), JUN (0.1 μg), FOS (0.1 μg), CBP (1 μg), SRC1 (1 μg) or increasing doses of SRC-1, CBP or SRC1 plus CBP as indicated by the table under the graph. Results represent the mean + s.e.m. of data from three independent experiments, each performed in triplicate. *P<0.001 and **P<0.05 compared with SF-1+JUN/FOS; ^P<0.05 compared with SF-1.

    Journal: Journal of Cell Science

    Article Title: The AP-1 family member FOS blocks transcriptional activity of the nuclear receptor steroidogenic factor 1

    doi: 10.1242/jcs.055806

    Figure Lengend Snippet: FOS competes with co-factors binding to SF-1 on the CYP17 promoter. (A–C) In vivo binding of SF-1 (A) to the CYP17 promoter was examined using a ChIP assay. Interaction of SRC1 (B) and CBP (C) to SF-1 on the CYP17 promoter was tested with a re-ChIP assay. All samples were immunoprecipitated with an anti-SF-1 antibody. For re-ChIP, samples were re-precipitated with an anti-SRC1 or anti-CBP antibody. Immunoprecipitated (SF-1, SF-1/SRC1, SF-1/CBP) and total (10% input) DNA were subject to real-time RT-PCR using specific primers. Ct values from immunoprecipitated samples were normalized to the input Ct values. Results represent the mean + s.e.m. of data from 3 independent experiments (*P<0.001 compared with basal and **P<0.001 compared with TPA). (D) H295R cells were transfected with the luciferase promoter construct containing 381 bp of the promoter region of the human CYP17 gene in the presence of the expression vector for SF-1 (0.3 μg), JUN (0.1 μg), FOS (0.1 μg), CBP (1 μg), SRC1 (1 μg) or increasing doses of SRC-1, CBP or SRC1 plus CBP as indicated by the table under the graph. Results represent the mean + s.e.m. of data from three independent experiments, each performed in triplicate. *P<0.001 and **P<0.05 compared with SF-1+JUN/FOS; ^P<0.05 compared with SF-1.

    Article Snippet: For immunohistochemistry, tissues were blocked with normal goat serum followed by incubation overnight with rabbit anti-CYP17 antibody (1/2000) and rabbit anti-FOS antibody (Calbiochem, 1/500).

    Techniques: Binding Assay, In Vivo, Immunoprecipitation, Quantitative RT-PCR, Transfection, Luciferase, Construct, Expressing, Plasmid Preparation