Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Article Title: Role of GRK4 in the regulation of the renal ETB receptor in hypertension
Figure Lengend Snippet: Effect of the intrarenal arterial infusion of the ETBR agonist BQ3020 on urine flow and sodium excretion in hGRK4γ WT and hGRK4γ 142V transgenic mice. A, Systolic, diastolic, and mean arterial blood pressures (SBP, DBP, and MBP, respectively), measured under pentobarbital anesthesia in hGRK4γ WT and hGRK4γ 142V transgenic mice, *P < .05 vs WT (N = 5/group). B and C, Basal urine flow (24 hours, V) and basal absolute sodium excretion (24 hours, UNaV). P = NS (N = 5/group). D and E, Urine flow (V) and absolute sodium excretion (UNaV). Varying doses (0.1, 0.5, 1.0 μg/kg/min) of ETBR agonist, BQ-3020 were infused at a rate of 0.04 mL/min for 40 minutes, preceded by a control period (40 minutes), and followed by a recovery period (40 minutes) in which the vehicle, normal saline, alone, was infused via the right supra-renal artery of anesthetized mice. #P < .05 vs control. *P < .05 vs WT (N = 5/group). F, Renal cortical ETBR phosphorylation determined by coimmunoprecipitation with immunoblotting. Renal cortical lysates were prepared (renal cortex of hGRK4γ 142V transgenic mice for negative and positive control group), and mouse anti-phosphoserine antibody was used for immunoprecipitation, with normal mouse IgG as negative control, and mouse anti-ETBR antibody as positive control. The immune complexes were precipitated using agarose-A/G beads and immunoblotted for ETBR. NC indicates negative control and PC indicates positive control. *P < .05 vs WT (N = 4/group). Data are expressed as Mean ± SE
Article Snippet: The immunoprecipitates were subjected to immunoblotting with rabbit anti-ETBR antibody (1:1000; Abcam) or anti-phosphoserine antibody.
Techniques: Transgenic Assay, Western Blot, Positive Control, Immunoprecipitation, Negative Control