qubit rna hs assay kit Thermo Fisher Search Results


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  • 99
    Thermo Fisher qubit rna high sensitivity hs assay kit
    Disorganization of cardiac muscles and abnormalities of cardiomyocyte nuclei in Rrm1 KO hearts. A , images from the LVs of P7 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. B , images from the LVs of P10 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. C , quantification of the cardiomyocyte nuclear density in the samples in A and B . Data are shown as median values ( horizontal bars ) from two WT pups and two Rrm1 KO pups at P7 and from four WT pups and six Rrm1 KO pups at P10. The p values were computed with Mann–Whitney U tests: ns , nonsignificant. D , LVs of P7 or P14 hearts were stained with DAPI. Scale bar , 10 μm. E , proportions of <t>DNA</t> and <t>RNA</t> per total extracted nucleic acid from the hearts of P7, P9, and P15 pups. Data are shown as means ± S.E. ( error bars ) with n = 6–9. The p values were computed with an unpaired t test with Welch's correction: ****, p
    Qubit Rna High Sensitivity Hs Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher assays qubit rna hs assay kit thermo fisher scientific
    Disorganization of cardiac muscles and abnormalities of cardiomyocyte nuclei in Rrm1 KO hearts. A , images from the LVs of P7 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. B , images from the LVs of P10 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. C , quantification of the cardiomyocyte nuclear density in the samples in A and B . Data are shown as median values ( horizontal bars ) from two WT pups and two Rrm1 KO pups at P7 and from four WT pups and six Rrm1 KO pups at P10. The p values were computed with Mann–Whitney U tests: ns , nonsignificant. D , LVs of P7 or P14 hearts were stained with DAPI. Scale bar , 10 μm. E , proportions of <t>DNA</t> and <t>RNA</t> per total extracted nucleic acid from the hearts of P7, P9, and P15 pups. Data are shown as means ± S.E. ( error bars ) with n = 6–9. The p values were computed with an unpaired t test with Welch's correction: ****, p
    Assays Qubit Rna Hs Assay Kit Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dsdna hs high sensitivity kit
    Disorganization of cardiac muscles and abnormalities of cardiomyocyte nuclei in Rrm1 KO hearts. A , images from the LVs of P7 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. B , images from the LVs of P10 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. C , quantification of the cardiomyocyte nuclear density in the samples in A and B . Data are shown as median values ( horizontal bars ) from two WT pups and two Rrm1 KO pups at P7 and from four WT pups and six Rrm1 KO pups at P10. The p values were computed with Mann–Whitney U tests: ns , nonsignificant. D , LVs of P7 or P14 hearts were stained with DAPI. Scale bar , 10 μm. E , proportions of <t>DNA</t> and <t>RNA</t> per total extracted nucleic acid from the hearts of P7, P9, and P15 pups. Data are shown as means ± S.E. ( error bars ) with n = 6–9. The p values were computed with an unpaired t test with Welch's correction: ****, p
    Dsdna Hs High Sensitivity Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher qubit high sensitivity rna assay kit
    Disorganization of cardiac muscles and abnormalities of cardiomyocyte nuclei in Rrm1 KO hearts. A , images from the LVs of P7 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. B , images from the LVs of P10 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. C , quantification of the cardiomyocyte nuclear density in the samples in A and B . Data are shown as median values ( horizontal bars ) from two WT pups and two Rrm1 KO pups at P7 and from four WT pups and six Rrm1 KO pups at P10. The p values were computed with Mann–Whitney U tests: ns , nonsignificant. D , LVs of P7 or P14 hearts were stained with DAPI. Scale bar , 10 μm. E , proportions of <t>DNA</t> and <t>RNA</t> per total extracted nucleic acid from the hearts of P7, P9, and P15 pups. Data are shown as means ± S.E. ( error bars ) with n = 6–9. The p values were computed with an unpaired t test with Welch's correction: ****, p
    Qubit High Sensitivity Rna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit high sensitivity rna assay kit/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
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    91
    Thermo Fisher qubit hs rna kit
    Disorganization of cardiac muscles and abnormalities of cardiomyocyte nuclei in Rrm1 KO hearts. A , images from the LVs of P7 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. B , images from the LVs of P10 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. C , quantification of the cardiomyocyte nuclear density in the samples in A and B . Data are shown as median values ( horizontal bars ) from two WT pups and two Rrm1 KO pups at P7 and from four WT pups and six Rrm1 KO pups at P10. The p values were computed with Mann–Whitney U tests: ns , nonsignificant. D , LVs of P7 or P14 hearts were stained with DAPI. Scale bar , 10 μm. E , proportions of <t>DNA</t> and <t>RNA</t> per total extracted nucleic acid from the hearts of P7, P9, and P15 pups. Data are shown as means ± S.E. ( error bars ) with n = 6–9. The p values were computed with an unpaired t test with Welch's correction: ****, p
    Qubit Hs Rna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Disorganization of cardiac muscles and abnormalities of cardiomyocyte nuclei in Rrm1 KO hearts. A , images from the LVs of P7 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. B , images from the LVs of P10 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. C , quantification of the cardiomyocyte nuclear density in the samples in A and B . Data are shown as median values ( horizontal bars ) from two WT pups and two Rrm1 KO pups at P7 and from four WT pups and six Rrm1 KO pups at P10. The p values were computed with Mann–Whitney U tests: ns , nonsignificant. D , LVs of P7 or P14 hearts were stained with DAPI. Scale bar , 10 μm. E , proportions of DNA and RNA per total extracted nucleic acid from the hearts of P7, P9, and P15 pups. Data are shown as means ± S.E. ( error bars ) with n = 6–9. The p values were computed with an unpaired t test with Welch's correction: ****, p

    Journal: The Journal of Biological Chemistry

    Article Title: De novo dNTP production is essential for normal postnatal murine heart development

    doi: 10.1074/jbc.RA119.009492

    Figure Lengend Snippet: Disorganization of cardiac muscles and abnormalities of cardiomyocyte nuclei in Rrm1 KO hearts. A , images from the LVs of P7 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. B , images from the LVs of P10 hearts stained with phalloidin and DAPI. Scale bar , 20 μm. C , quantification of the cardiomyocyte nuclear density in the samples in A and B . Data are shown as median values ( horizontal bars ) from two WT pups and two Rrm1 KO pups at P7 and from four WT pups and six Rrm1 KO pups at P10. The p values were computed with Mann–Whitney U tests: ns , nonsignificant. D , LVs of P7 or P14 hearts were stained with DAPI. Scale bar , 10 μm. E , proportions of DNA and RNA per total extracted nucleic acid from the hearts of P7, P9, and P15 pups. Data are shown as means ± S.E. ( error bars ) with n = 6–9. The p values were computed with an unpaired t test with Welch's correction: ****, p

    Article Snippet: DNA and RNA concentrations were measured using the Qubit dsDNA HS and RNA HS assay kits, respectively, and a Qubit 2.0 fluorometer (Molecular Probes).

    Techniques: Staining, MANN-WHITNEY

    Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.

    Journal: Scientific Reports

    Article Title: Comparative evaluation of rRNA depletion procedures for the improved analysis of bacterial biofilm and mixed pathogen culture transcriptomes

    doi: 10.1038/srep41114

    Figure Lengend Snippet: Quantitative and qualitative comparison of RNA recovered following rRNA depletion. A total of 4 μg of DNAse-treated RNA, isolated form 3-day-old P. aeruginosa PAO1 biofilms, was subjected to treatment with the Illumina Ribo-Zero rRNA Removal Kit (Bacteria), Ambion MICROBExpress™ Bacterial mRNA Enrichment Kit and the Life Technologies RiboMinus Transcriptome Isolation Kit, Bacteria. Following rRNA depletion and ethanol/acetate precipitation and resuspension in equal volumes of water, the RNA was assessed using Qubit fluorimetric quantitation with the Qubit RNA HS Assay Kit. Yields are reported as total RNA recovered ( A ) and as percentage of the input RNA ( B ). The RNA samples were also assessed using the Bioanalyzer RNA 6000 Pico kit. Representative electropherograms of ( C ) starting total RNA material and aliquots of the RNA samples that have been processed using the ( D ) MICROBExpress, ( E ) RiboMinus, or ( F ) Ribo-Zero kits are shown. Dashed lines indicate peaks corresponding to 16S and 23S RNA traces, which were detected in total RNA, MICROBExpress, and RiboMins samples. ( G ) The area of 16S and 23S rRNA peaks as percent of the total detected RNA was estimated, as determined using the 2100 Expert Software. RFU, relative fluorescence units. Experiments were repeated using three biological replicates.

    Article Snippet: RNA and DNA quantity and quality assessment Where indicated, RNA or DNA was quantified using Qubit RNA HS Assay Kit and Qubit dsDNA HS Assay Kit (Life Technologies), respectively, on the Qubit 2.0 Fluorometer (Life Technologies).

    Techniques: Isolation, Quantitation Assay, RNA HS Assay, Software, Fluorescence