qubit fluorometer Search Results


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  • 99
    Thermo Fisher qubit3 fluorometer
    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p
    Qubit3 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit3 fluorometer/product/Thermo Fisher
    Average 99 stars, based on 122 article reviews
    Price from $9.99 to $1999.99
    qubit3 fluorometer - by Bioz Stars, 2020-04
    99/100 stars
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    99
    Thermo Fisher qubit4 fluorometer
    Scatter plots showing comparison of cell-free DNA estimation by NanoDrop and Qubit 2.0NanoDrop and Qubit 2.0
    Qubit4 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 682 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit4 fluorometer/product/Thermo Fisher
    Average 99 stars, based on 682 article reviews
    Price from $9.99 to $1999.99
    qubit4 fluorometer - by Bioz Stars, 2020-04
    99/100 stars
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    99
    Thermo Fisher qubit fluorometer
    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with <t>Qubit.</t> b DNA quantified with <t>NanoDrop.</t> c DNA quality determined by A260/A280 ratio. d DNA quality
    Qubit Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20330 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer/product/Thermo Fisher
    Average 99 stars, based on 20330 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometer - by Bioz Stars, 2020-04
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    93
    Fisher Scientific qubit fluorometer
    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with <t>Qubit.</t> b DNA quantified with <t>NanoDrop.</t> c DNA quality determined by A260/A280 ratio. d DNA quality
    Qubit Fluorometer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer/product/Fisher Scientific
    Average 93 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometer - by Bioz Stars, 2020-04
    93/100 stars
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    94
    Promega qubit fluorometer
    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with <t>Qubit.</t> b DNA quantified with <t>NanoDrop.</t> c DNA quality determined by A260/A280 ratio. d DNA quality
    Qubit Fluorometer, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer/product/Promega
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
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    90
    Thermo Fisher qubit fluorometers
    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with <t>Qubit.</t> b DNA quantified with <t>NanoDrop.</t> c DNA quality determined by A260/A280 ratio. d DNA quality
    Qubit Fluorometers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometers/product/Thermo Fisher
    Average 90 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
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    93
    Bio-Rad qubit fluorometer
    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with <t>Qubit.</t> b DNA quantified with <t>NanoDrop.</t> c DNA quality determined by A260/A280 ratio. d DNA quality
    Qubit Fluorometer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer/product/Bio-Rad
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometer - by Bioz Stars, 2020-04
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    99
    Agilent technologies qubit fluorometer
    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with <t>Qubit.</t> b DNA quantified with <t>NanoDrop.</t> c DNA quality determined by A260/A280 ratio. d DNA quality
    Qubit Fluorometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer/product/Agilent technologies
    Average 99 stars, based on 781 article reviews
    Price from $9.99 to $1999.99
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    99
    Illumina Inc qubit fluorometer
    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with <t>Qubit.</t> b DNA quantified with <t>NanoDrop.</t> c DNA quality determined by A260/A280 ratio. d DNA quality
    Qubit Fluorometer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer/product/Illumina Inc
    Average 99 stars, based on 262 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometer - by Bioz Stars, 2020-04
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    92
    Turner BioSystems qubit fluorometer
    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with <t>Qubit.</t> b DNA quantified with <t>NanoDrop.</t> c DNA quality determined by A260/A280 ratio. d DNA quality
    Qubit Fluorometer, supplied by Turner BioSystems, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer/product/Turner BioSystems
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher qubit fluorimeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorimeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1819 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorimeter/product/Thermo Fisher
    Average 99 stars, based on 1819 article reviews
    Price from $9.99 to $1999.99
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    93
    Thermo Fisher qubit fluoremeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluoremeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluoremeter/product/Thermo Fisher
    Average 93 stars, based on 79 article reviews
    Price from $9.99 to $1999.99
    qubit fluoremeter - by Bioz Stars, 2020-04
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    94
    Thermo Fisher qubit fluorometer system
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometer System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometer system/product/Thermo Fisher
    Average 94 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometer system - by Bioz Stars, 2020-04
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    85
    Thermo Fisher qubit fluorormeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorormeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    87
    Thermo Fisher qubit fluoromoeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluoromoeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluoromoeter/product/Thermo Fisher
    Average 87 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    qubit fluoromoeter - by Bioz Stars, 2020-04
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    96
    Thermo Fisher qubit fluorometr
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometr/product/Thermo Fisher
    Average 96 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
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    97
    Agilent technologies qubit fluorimeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorimeter, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorimeter/product/Agilent technologies
    Average 97 stars, based on 91 article reviews
    Price from $9.99 to $1999.99
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    94
    Thermo Fisher qubitr fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubitr Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 13 article reviews
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    94
    Thermo Fisher qubit v1 fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit V1 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit v1 fluorometer/product/Thermo Fisher
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    qubit v1 fluorometer - by Bioz Stars, 2020-04
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    94
    Thermo Fisher picogreen qubit fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Picogreen Qubit Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/picogreen qubit fluorometer/product/Thermo Fisher
    Average 94 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    picogreen qubit fluorometer - by Bioz Stars, 2020-04
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    94
    Thermo Fisher 9001941 qubit fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    9001941 Qubit Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/9001941 qubit fluorometer/product/Thermo Fisher
    Average 94 stars, based on 3 article reviews
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    9001941 qubit fluorometer - by Bioz Stars, 2020-04
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    92
    Thermo Fisher qubit2 fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit2 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit2 fluorometer/product/Thermo Fisher
    Average 92 stars, based on 25 article reviews
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    97
    Thermo Fisher qubit fluorometric system
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometric System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometric system/product/Thermo Fisher
    Average 97 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    qubit fluorometric system - by Bioz Stars, 2020-04
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    94
    Thermo Fisher qubit fluorometric spectrophotometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometric Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorometric spectrophotometer/product/Thermo Fisher
    Average 94 stars, based on 23 article reviews
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    qubit fluorometric spectrophotometer - by Bioz Stars, 2020-04
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    80
    Thermo Fisher qubit digital fluorimeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Digital Fluorimeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit digital fluorimeter/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
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    88
    Thermo Fisher qubit fluorometric quantitiation
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometric Quantitiation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 25 article reviews
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    92
    Millipore qubit 2 0 fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit 2 0 Fluorometer, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit 2 0 fluorometer/product/Millipore
    Average 92 stars, based on 5 article reviews
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    qubit 2 0 fluorometer - by Bioz Stars, 2020-04
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    92
    Thermo Fisher qubitr fluorimeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubitr Fluorimeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubitr fluorimeter/product/Thermo Fisher
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    qubitr fluorimeter - by Bioz Stars, 2020-04
    92/100 stars
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    95
    Thermo Fisher qubit 2 benchtop fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit 2 Benchtop Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit 2 benchtop fluorometer/product/Thermo Fisher
    Average 95 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    qubit 2 benchtop fluorometer - by Bioz Stars, 2020-04
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    94
    Thermo Fisher qubit v3 0 fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit V3 0 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit v3 0 fluorometer/product/Thermo Fisher
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    qubit v3 0 fluorometer - by Bioz Stars, 2020-04
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    86
    Thermo Fisher qubit 1 27 fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit 1 27 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 4 article reviews
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    Image Search Results


    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p

    Journal: Cancers

    Article Title: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics

    doi: 10.3390/cancers11111691

    Figure Lengend Snippet: Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) . Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. * p

    Article Snippet: Library quantification was assessed using a Qubit® 3.0 Fluorometer (Thermo Fisher, Waltham, MA, USA) using the Qubit dsDNA HS (high sensitivity) assay kit (Life Technology, Thermo Fisher Scientific).

    Techniques: DNA Extraction, Concentration Assay, Spectrophotometry

    Comparison of two automatic DNA extraction systems (OMNIA Prima and King Fisher Duo). DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ). DNA (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p

    Journal: Cancers

    Article Title: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics

    doi: 10.3390/cancers11111691

    Figure Lengend Snippet: Comparison of two automatic DNA extraction systems (OMNIA Prima and King Fisher Duo). DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ). DNA (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. * p

    Article Snippet: Library quantification was assessed using a Qubit® 3.0 Fluorometer (Thermo Fisher, Waltham, MA, USA) using the Qubit dsDNA HS (high sensitivity) assay kit (Life Technology, Thermo Fisher Scientific).

    Techniques: DNA Extraction, Concentration Assay, Spectrophotometry

    DNA yield and  rbcL  copies extracted during phase 3. DNA yield (top) calculated using a Qubit 1.0 Fluorometer and  rbcL  copies (bottom) determined by qPCR. Values are scaled to the maximum value of each sample, with the highest value listed above the corresponding bar. Missing bars in lower portion of figure indicates that a sample did not amplify in qPCR.

    Journal: PLoS ONE

    Article Title: Optimization of DNA Recovery and Amplification from Non-Carbonized Archaeobotanical Remains

    doi: 10.1371/journal.pone.0086827

    Figure Lengend Snippet: DNA yield and rbcL copies extracted during phase 3. DNA yield (top) calculated using a Qubit 1.0 Fluorometer and rbcL copies (bottom) determined by qPCR. Values are scaled to the maximum value of each sample, with the highest value listed above the corresponding bar. Missing bars in lower portion of figure indicates that a sample did not amplify in qPCR.

    Article Snippet: Extraction methods were compared on the basis of three criteria: DNA concentration measured on a Qubit 1.0 Fluorometer (Invitrogen, Carlsbad, CA), sample purity measured on a NanoDrop 1000 spectrophotomer (Thermo Scientific, Waltham, MA), and amplification success for the ribulose-bisphosphate carboxylase (rbcL ) gene, a universal plant marker .

    Techniques: Real-time Polymerase Chain Reaction

    Scatter plots showing comparison of cell-free DNA estimation by NanoDrop and Qubit 2.0NanoDrop and Qubit 2.0

    Journal: Asian Journal of Transfusion Science

    Article Title: Comparison of UV spectrometry and fluorometry-based methods for quantification of cell-free DNA in red cell components

    doi: 10.4103/ajts.AJTS_90_19

    Figure Lengend Snippet: Scatter plots showing comparison of cell-free DNA estimation by NanoDrop and Qubit 2.0NanoDrop and Qubit 2.0

    Article Snippet: Cell-free DNA quantification using benchtop fluorometer Estimation of cell-free DNA (cfDNA) was done using 10 μL of extracted DNA sample on Qubit 2.0 Fluorometer (Life Technologies, ThermoFisher Scientific, USA) using the Qubit dsDNA HS Assay Kit as per manufacturer's instruction.

    Techniques:

    Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA DNA yields from the individual U-2 OS cells and the respective controls, as measured by Qubit ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.

    Journal: PLoS ONE

    Article Title: Molecular Genetic Characterization of Individual Cancer Cells Isolated via Single-Cell Printing

    doi: 10.1371/journal.pone.0163455

    Figure Lengend Snippet: Single-cell whole genome amplification and sequencing of the U-2 OS cell line. (A) Bar diagram displaying the WGA DNA yields from the individual U-2 OS cells and the respective controls, as measured by Qubit ™ . (B) Agarose gel illustrating the differently sized products of the LINE1 multiplex PCR that was performed on the WGA DNA of the individual U-2 OS cells. (C) Exemplary sequencing chromatograms of the SLC34A2 and TET2 gene mutations in the cell bulk and individual cells. (D) Conclusions on the occurrence of allelic dropout (ADO) through sequencing of single nucleotide polymorphisms (SNPs). SNPs rs1391438 and rs7655890 are located in close genomic proximity to the TET2 mutation and show heterozygous patterns in the cell bulk (left). In the single U-2 OS cells B8 and C10, wild-type only is detected at the TET2 mutation site. The heterozygous patterns of the SNPs in B8 suggest true wild-type in TET2 , while the detection of only one allele of both SNPs in C10 suggest loss of the genomic region due to ADO. NTC: no-template control, PTC: positive control.

    Article Snippet: The DNA yield was assessed by Qubit fluorometric quantitation (Thermo Fisher Scientific) according to the manufacturer’s protocol.

    Techniques: Whole Genome Amplification, Sequencing, Agarose Gel Electrophoresis, Multiplex Assay, Polymerase Chain Reaction, Mutagenesis, Positive Control

    Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with Qubit. b DNA quantified with NanoDrop. c DNA quality determined by A260/A280 ratio. d DNA quality

    Journal: 3 Biotech

    Article Title: Biases during DNA extraction affect bacterial and archaeal community profile of anaerobic digestion samples

    doi: 10.1007/s13205-017-1009-x

    Figure Lengend Snippet: Yield and quality of DNA extracted from samples obtained from the inlet, digester and slurry using various methods of DNA extraction. a DNA quantified with Qubit. b DNA quantified with NanoDrop. c DNA quality determined by A260/A280 ratio. d DNA quality

    Article Snippet: DNA yield was measured using two methods: Nanodrop (Nanodrop One, Thermoscientific, USA) and the Qubit fluorometer (Invitrogen, USA, using the Qubit® dsDNA HS Assay Kit).

    Techniques: DNA Extraction

    RNA concentrations, as determined by Qubit, correlated with values determined by NanoVue (r=0.827, p

    Journal: Journal of Neurotrauma

    Article Title: Anesthesia for Euthanasia Influences mRNA Expression in Healthy Mice and after Traumatic Brain Injury

    doi: 10.1089/neu.2013.3243

    Figure Lengend Snippet: RNA concentrations, as determined by Qubit, correlated with values determined by NanoVue (r=0.827, p

    Article Snippet: RNA concentration was calculated using the Quanti-iT™ RNA Assay Kit with the Qubit™ fluorimeter (Molecular Probes,™ Invitrogen, Karlsruhe, Germany) and with the NanoVue system (GE Healthcare Europe, Munich, Germany).

    Techniques: