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  • 99
    Thermo Fisher qubit fluorimeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorimeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorimeter/product/Thermo Fisher
    Average 99 stars, based on 2223 article reviews
    Price from $9.99 to $1999.99
    qubit fluorimeter - by Bioz Stars, 2019-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher qubit 3 fluorometer
    a  3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [  8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth.  b  Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5  ×  10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [  40 ].  c  Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls
    Qubit 3 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit 3 fluorometer/product/Thermo Fisher
    Average 99 stars, based on 1438 article reviews
    Price from $9.99 to $1999.99
    qubit 3 fluorometer - by Bioz Stars, 2019-10
    99/100 stars
      Buy from Supplier

    79
    Thermo Fisher qubit 2 0 fluorometer measurements
    Random hexamer primer detection by a dsDNA-specific fluorometric assay. Random hexamers (250, 500, 1000, 2000, 4000 ng) were added to 200 μL of the PureLyse elution buffer, homogenized, then quantified on a Qubit 2.0 fluorometer (standard error shown,  n  = 3). Random hexamer loads were not detectable until 20 ng/μL (4000 ng in 200 μL), likely due to increased hybridization. These results suggest that using random hexamer primers (less than 20 ng/μL) as competitive binders will not bias DNA yield results with this dsDNA-specific fluorometric assay. *At or below 0.01 ng limit of detection.
    Qubit 2 0 Fluorometer Measurements, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit 2 0 fluorometer measurements/product/Thermo Fisher
    Average 79 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    qubit 2 0 fluorometer measurements - by Bioz Stars, 2019-10
    79/100 stars
      Buy from Supplier

    Image Search Results


    RNA concentrations, as determined by Qubit, correlated with values determined by NanoVue (r=0.827, p

    Journal:

    Article Title: Anesthesia for Euthanasia Influences mRNA Expression in Healthy Mice and after Traumatic Brain Injury

    doi: 10.1089/neu.2013.3243

    Figure Lengend Snippet: RNA concentrations, as determined by Qubit, correlated with values determined by NanoVue (r=0.827, p

    Article Snippet: RNA concentration was calculated using the Quanti-iT™ RNA Assay Kit with the Qubit™ fluorimeter (Molecular Probes,™ Invitrogen, Karlsruhe, Germany) and with the NanoVue system (GE Healthcare Europe, Munich, Germany).

    Techniques:

    Mean (±SEM) DNA concentration (nM) of PCR products, measured by Qubit fluorometer, of the six template categories (Mammalia, Aves, Reptilia, Amphibia, Mosquito, negative control [No DNA]) by three primer combinations (Mod_RepCOI_F + Mod_RepCOI_R, VertCOI_7194_F + Mod_RepCOI_R, Mod_RepCOI_F + VertCOI_7216_R). Tukey’s HSD test detected pairwise differences between mean DNA concentration for each primer combination and host class. Significant differences between groups are indicated by letters. Mean DNA concentration of groups that have the same letter are not significantly different from each other, while mean DNA concentration of groups that do not share a common letter are significantly different. Comparisons were considered significant if P

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Barcoding blood meals: New vertebrate-specific primer sets for assigning taxonomic identities to host DNA from mosquito blood meals

    doi: 10.1371/journal.pntd.0006767

    Figure Lengend Snippet: Mean (±SEM) DNA concentration (nM) of PCR products, measured by Qubit fluorometer, of the six template categories (Mammalia, Aves, Reptilia, Amphibia, Mosquito, negative control [No DNA]) by three primer combinations (Mod_RepCOI_F + Mod_RepCOI_R, VertCOI_7194_F + Mod_RepCOI_R, Mod_RepCOI_F + VertCOI_7216_R). Tukey’s HSD test detected pairwise differences between mean DNA concentration for each primer combination and host class. Significant differences between groups are indicated by letters. Mean DNA concentration of groups that have the same letter are not significantly different from each other, while mean DNA concentration of groups that do not share a common letter are significantly different. Comparisons were considered significant if P

    Article Snippet: For each PCR product, the remaining volume was sent to the University of Florida, Interdisciplinary Center for Biotechnology Research (ICBR) for DNA quantification by Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Concentration Assay, Polymerase Chain Reaction, Negative Control, IF-P

    Mean (±SEM) DNA concentration (nM) of PCR products, measured by Qubit fluorometer, of the six template categories (Mammalia, Aves, Reptilia, Amphibia, Mosquito, negative control [No DNA]) by three primer combinations (Mod_RepCOI_F + Mod_RepCOI_R, VertCOI_7194_F + Mod_RepCOI_R, Mod_RepCOI_F + VertCOI_7216_R). Tukey’s HSD test detected pairwise differences between mean DNA concentration for each primer combination and host class. Significant differences between groups are indicated by letters. Mean DNA concentration of groups that have the same letter are not significantly different from each other, while mean DNA concentration of groups that do not share a common letter are significantly different. Comparisons were considered significant if P < 0.05. Error bars represent standard error of the mean.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Barcoding blood meals: New vertebrate-specific primer sets for assigning taxonomic identities to host DNA from mosquito blood meals

    doi: 10.1371/journal.pntd.0006767

    Figure Lengend Snippet: Mean (±SEM) DNA concentration (nM) of PCR products, measured by Qubit fluorometer, of the six template categories (Mammalia, Aves, Reptilia, Amphibia, Mosquito, negative control [No DNA]) by three primer combinations (Mod_RepCOI_F + Mod_RepCOI_R, VertCOI_7194_F + Mod_RepCOI_R, Mod_RepCOI_F + VertCOI_7216_R). Tukey’s HSD test detected pairwise differences between mean DNA concentration for each primer combination and host class. Significant differences between groups are indicated by letters. Mean DNA concentration of groups that have the same letter are not significantly different from each other, while mean DNA concentration of groups that do not share a common letter are significantly different. Comparisons were considered significant if P < 0.05. Error bars represent standard error of the mean.

    Article Snippet: For each PCR product, the remaining volume was sent to the University of Florida, Interdisciplinary Center for Biotechnology Research (ICBR) for DNA quantification by Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Concentration Assay, Polymerase Chain Reaction, Negative Control, IF-P

    DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER

    Journal: PLoS ONE

    Article Title: Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing

    doi: 10.1371/journal.pone.0135058

    Figure Lengend Snippet: DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER

    Article Snippet: For the determination of the DNA yield after bisulfite conversion, the Qubit fluorometer (Invitrogen) was used according to manufacturer's instructions.

    Techniques: Modification

    Plasmid DNA yield. E . coli DH5α and JM109 were transformed with high-copy number pUC19-Bla, pUC19-FabV, pUC19-FabI, and medium-copy number pSA-Hp24-Bla, pSA-Hp24-FabV, pBR322-FabI plasmids and selected on LB agar plates containing 1μM Triclosan (for FabV/FabI plasmids) and or 100μg mL -1 ampicillin (for Bla plasmids). Seed cultures were used to inoculated 5mL LB broth in 50mL flasks and cultured for another 18 hours at 37 or 30°C while shaking at 250rpm. Cell density was then measured by absorbing the diluted samples at 600nm and normalized to 2 OD600. One mL of the normalized culture was then used to extract the plasmid following supplied protocol. The quantity of the extracted plasmid DNA was measured by fluorometry using Qubit Fluorometer and Qubit dsDNA BR Assay Kit (Invitrogen, life technology). Fig 4A shows plasmid DNA yield when bacteria were grown at 37°C, whereas Fig 4B shows the plasmid DNA yield from cultures incubated at 30°C. Error bars show standard deviations calculated from at-least six (6) independent experiments performed in triplicate.

    Journal: PLoS ONE

    Article Title: FabV/Triclosan Is an Antibiotic-Free and Cost-Effective Selection System for Efficient Maintenance of High and Medium -Copy Number Plasmids in Escherichia coli

    doi: 10.1371/journal.pone.0129547

    Figure Lengend Snippet: Plasmid DNA yield. E . coli DH5α and JM109 were transformed with high-copy number pUC19-Bla, pUC19-FabV, pUC19-FabI, and medium-copy number pSA-Hp24-Bla, pSA-Hp24-FabV, pBR322-FabI plasmids and selected on LB agar plates containing 1μM Triclosan (for FabV/FabI plasmids) and or 100μg mL -1 ampicillin (for Bla plasmids). Seed cultures were used to inoculated 5mL LB broth in 50mL flasks and cultured for another 18 hours at 37 or 30°C while shaking at 250rpm. Cell density was then measured by absorbing the diluted samples at 600nm and normalized to 2 OD600. One mL of the normalized culture was then used to extract the plasmid following supplied protocol. The quantity of the extracted plasmid DNA was measured by fluorometry using Qubit Fluorometer and Qubit dsDNA BR Assay Kit (Invitrogen, life technology). Fig 4A shows plasmid DNA yield when bacteria were grown at 37°C, whereas Fig 4B shows the plasmid DNA yield from cultures incubated at 30°C. Error bars show standard deviations calculated from at-least six (6) independent experiments performed in triplicate.

    Article Snippet: The quantity of the extracted plasmid DNA was measured by fluorometry using Qubit Fluorometer and Qubit dsDNA BR Assay Kit (Invitrogen, life technology).

    Techniques: Plasmid Preparation, Transformation Assay, Cell Culture, Incubation

    a  3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [  8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth.  b  Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5  ×  10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [  40 ].  c  Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls

    Journal: BMC Cancer

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine

    doi: 10.1186/s12885-016-2930-9

    Figure Lengend Snippet: a 3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [ 8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth. b Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5  ×  10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [ 40 ]. c Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls

    Article Snippet: Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852).

    Techniques: RNA Extraction, Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction

    Random hexamer primer detection by a dsDNA-specific fluorometric assay. Random hexamers (250, 500, 1000, 2000, 4000 ng) were added to 200 μL of the PureLyse elution buffer, homogenized, then quantified on a Qubit 2.0 fluorometer (standard error shown,  n  = 3). Random hexamer loads were not detectable until 20 ng/μL (4000 ng in 200 μL), likely due to increased hybridization. These results suggest that using random hexamer primers (less than 20 ng/μL) as competitive binders will not bias DNA yield results with this dsDNA-specific fluorometric assay. *At or below 0.01 ng limit of detection.

    Journal: Astrobiology

    Article Title: Nucleic Acid Extraction from Synthetic Mars Analog Soils for in situ Life Detection

    doi: 10.1089/ast.2016.1535

    Figure Lengend Snippet: Random hexamer primer detection by a dsDNA-specific fluorometric assay. Random hexamers (250, 500, 1000, 2000, 4000 ng) were added to 200 μL of the PureLyse elution buffer, homogenized, then quantified on a Qubit 2.0 fluorometer (standard error shown, n  = 3). Random hexamer loads were not detectable until 20 ng/μL (4000 ng in 200 μL), likely due to increased hybridization. These results suggest that using random hexamer primers (less than 20 ng/μL) as competitive binders will not bias DNA yield results with this dsDNA-specific fluorometric assay. *At or below 0.01 ng limit of detection.

    Article Snippet: DNAout was quantified using a double-stranded, DNA-specific fluorometric assay (Invitrogen, Qubit dsDNA HS Assay Kit, Q32854) and a Qubit 2.0 fluorometer (Invitrogen, Q32866, limit of detection of 0.1 ng/mL, dsDNA).

    Techniques: Random Hexamer Labeling, Hybridization

    PCR amplification and DNA concentration in decoction with sliced or pulverized sample at different times of boiling. (A) P. ginseng  sample was sliced (lanes 1–3, 7–9) or pulverized (lanes 4–6, 10–12) and boiled for 30 min (lanes 1, 4, 7, 10), 60 min (lanes 2, 5, 8, 11) and 120 min (lanes 3, 6, 9, 12). Crude DNA (lanes 1–6) and extracted DNA with concentration adjusted to that in the original decoction (lanes 7–12) were amplified by primer pair 9. Lane 13 is the positive control with DNA from  P. ginseng  and lane 14 is the negative control without DNA. Lane M represents the DNA size ladder.  (B)  The concentration of extracted  P. ginseng  DNA was adjusted to make it similar to that in the original decoction and measured by Qubit 2.0 Fluorometer. Water was used as blank and the data represent mean ± standard deviation (n = 3). Difference between boiling time was analyzed by one-way analysis of variance. Difference between sliced and pulverized samples at the same boiling time was analyzed by two sample  t  test (* p

    Journal: Chinese Medicine

    Article Title: Identification of constituent herbs in ginseng decoctions by DNA markers

    doi: 10.1186/s13020-015-0029-x

    Figure Lengend Snippet: PCR amplification and DNA concentration in decoction with sliced or pulverized sample at different times of boiling. (A) P. ginseng sample was sliced (lanes 1–3, 7–9) or pulverized (lanes 4–6, 10–12) and boiled for 30 min (lanes 1, 4, 7, 10), 60 min (lanes 2, 5, 8, 11) and 120 min (lanes 3, 6, 9, 12). Crude DNA (lanes 1–6) and extracted DNA with concentration adjusted to that in the original decoction (lanes 7–12) were amplified by primer pair 9. Lane 13 is the positive control with DNA from P. ginseng and lane 14 is the negative control without DNA. Lane M represents the DNA size ladder. (B) The concentration of extracted P. ginseng DNA was adjusted to make it similar to that in the original decoction and measured by Qubit 2.0 Fluorometer. Water was used as blank and the data represent mean ± standard deviation (n = 3). Difference between boiling time was analyzed by one-way analysis of variance. Difference between sliced and pulverized samples at the same boiling time was analyzed by two sample t test (* p

    Article Snippet: DNA concentration was measured using a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and presented as the mean ± standard deviation.

    Techniques: Polymerase Chain Reaction, Amplification, Concentration Assay, Positive Control, Negative Control, Standard Deviation

    Comparison of five automated DNA extraction systems. Illustration of relative DNA concentrations of samples 1–10 measured by the NanoDrop 2000c spectrophotometer (A) and Qubit 2.0 fluorometer (B). The concentrations of the Maxwell 16 extracts were set to 100% for each sample.

    Journal: PLoS ONE

    Article Title: Comparison of Pre-Analytical FFPE Sample Preparation Methods and Their Impact on Massively Parallel Sequencing in Routine Diagnostics

    doi: 10.1371/journal.pone.0104566

    Figure Lengend Snippet: Comparison of five automated DNA extraction systems. Illustration of relative DNA concentrations of samples 1–10 measured by the NanoDrop 2000c spectrophotometer (A) and Qubit 2.0 fluorometer (B). The concentrations of the Maxwell 16 extracts were set to 100% for each sample.

    Article Snippet: Additionally, each DNA sample was quantified in duplicates with the Qubit 2.0 fluorometer (Life Technologies, Darmstadt, Germany).

    Techniques: DNA Extraction, Spectrophotometry