qubit fluorimeter Search Results


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  • 99
    Thermo Fisher qubit4 fluorometer
    <t>DNA</t> degradation. DNA recovery after bisulfite treatment was determined with the <t>Qubit</t> fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER
    Qubit4 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1455 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher qubit3 fluorometer
    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p
    Qubit3 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher qubit fluorimeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorimeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1821 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher qubit fluoremeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluoremeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad qubit fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega qubit fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometer, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Agilent technologies qubit fluorimeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorimeter, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific qubit fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometer, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Illumina Inc qubit fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher qubit fluorometers
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies qubit fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 781 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher qubit digital fluorimeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Digital Fluorimeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Turner BioSystems qubit fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometer, supplied by Turner BioSystems, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher qubitr fluorimeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubitr Fluorimeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher qubit fluorometer system
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometer System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher qubit fluorimetric quantitation
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorimetric Quantitation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher qubitr fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubitr Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher qubit v1 fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit V1 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher picogreen qubit fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Picogreen Qubit Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher 9001941 qubit fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    9001941 Qubit Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific qubit 3 0 fluorimeter
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit 3 0 Fluorimeter, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher qubit 2 benchtop fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit 2 Benchtop Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher qubit v3 0 fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit V3 0 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher qubit fluorometer 1 0
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Fluorometer 1 0, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Agilent technologies qubit 2 0 fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit 2 0 Fluorometer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 921 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore qubit 2 0 fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit 2 0 Fluorometer, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher qubit hsdna fluorimetric quantitation
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit Hsdna Fluorimetric Quantitation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher qubit 12 0 fluorometer
    <t>RNA</t> concentrations, as determined by <t>Qubit,</t> correlated with values determined by NanoVue (r=0.827, p
    Qubit 12 0 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER

    Journal: PLoS ONE

    Article Title: Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing

    doi: 10.1371/journal.pone.0135058

    Figure Lengend Snippet: DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER

    Article Snippet: DNA yield and degradation In order to compare the DNA yield and hence, the degradation effect of each of the four different kits we used the Qubit fluorometer (Invitrogen) to determine the bisulfite-treated DNA concentration.

    Techniques: Modification

    a  3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [  8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth.  b  Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5  ×  10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [  40 ].  c  Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls

    Journal: BMC Cancer

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine

    doi: 10.1186/s12885-016-2930-9

    Figure Lengend Snippet: a 3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [ 8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth. b Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5  ×  10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [ 40 ]. c Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls

    Article Snippet: Real-time and end-point RT-PCR Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852).

    Techniques: RNA Extraction, Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction

    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p

    Journal: Cancers

    Article Title: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics

    doi: 10.3390/cancers11111691

    Figure Lengend Snippet: Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) . Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. * p

    Article Snippet: Library quantification was assessed using a Qubit® 3.0 Fluorometer (Thermo Fisher, Waltham, MA, USA) using the Qubit dsDNA HS (high sensitivity) assay kit (Life Technology, Thermo Fisher Scientific).

    Techniques: DNA Extraction, Concentration Assay, Spectrophotometry

    Comparison of two automatic DNA extraction systems (OMNIA Prima and King Fisher Duo). DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ). DNA (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p

    Journal: Cancers

    Article Title: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics

    doi: 10.3390/cancers11111691

    Figure Lengend Snippet: Comparison of two automatic DNA extraction systems (OMNIA Prima and King Fisher Duo). DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ). DNA (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. * p

    Article Snippet: Library quantification was assessed using a Qubit® 3.0 Fluorometer (Thermo Fisher, Waltham, MA, USA) using the Qubit dsDNA HS (high sensitivity) assay kit (Life Technology, Thermo Fisher Scientific).

    Techniques: DNA Extraction, Concentration Assay, Spectrophotometry

    DNA yield and  rbcL  copies extracted during phase 3. DNA yield (top) calculated using a Qubit 1.0 Fluorometer and  rbcL  copies (bottom) determined by qPCR. Values are scaled to the maximum value of each sample, with the highest value listed above the corresponding bar. Missing bars in lower portion of figure indicates that a sample did not amplify in qPCR.

    Journal: PLoS ONE

    Article Title: Optimization of DNA Recovery and Amplification from Non-Carbonized Archaeobotanical Remains

    doi: 10.1371/journal.pone.0086827

    Figure Lengend Snippet: DNA yield and rbcL copies extracted during phase 3. DNA yield (top) calculated using a Qubit 1.0 Fluorometer and rbcL copies (bottom) determined by qPCR. Values are scaled to the maximum value of each sample, with the highest value listed above the corresponding bar. Missing bars in lower portion of figure indicates that a sample did not amplify in qPCR.

    Article Snippet: Extraction methods were compared on the basis of three criteria: DNA concentration measured on a Qubit 1.0 Fluorometer (Invitrogen, Carlsbad, CA), sample purity measured on a NanoDrop 1000 spectrophotomer (Thermo Scientific, Waltham, MA), and amplification success for the ribulose-bisphosphate carboxylase (rbcL ) gene, a universal plant marker .

    Techniques: Real-time Polymerase Chain Reaction

    RNA concentrations, as determined by Qubit, correlated with values determined by NanoVue (r=0.827, p

    Journal: Journal of Neurotrauma

    Article Title: Anesthesia for Euthanasia Influences mRNA Expression in Healthy Mice and after Traumatic Brain Injury

    doi: 10.1089/neu.2013.3243

    Figure Lengend Snippet: RNA concentrations, as determined by Qubit, correlated with values determined by NanoVue (r=0.827, p

    Article Snippet: RNA concentration was calculated using the Quanti-iT™ RNA Assay Kit with the Qubit™ fluorimeter (Molecular Probes,™ Invitrogen, Karlsruhe, Germany) and with the NanoVue system (GE Healthcare Europe, Munich, Germany).

    Techniques: