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  • 99
    Thermo Fisher qubit 2 0 fluorometer
    Qubit 2 0 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 34623 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit 2 0 fluorometer/product/Thermo Fisher
    Average 99 stars, based on 34623 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher qubit 3 0 fluorometer
    Qubit 3 0 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit 3 0 fluorometer/product/Thermo Fisher
    Average 99 stars, based on 5320 article reviews
    Price from $9.99 to $1999.99
    qubit 3 0 fluorometer - by Bioz Stars, 2021-01
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    99
    Thermo Fisher qubit rna assay kit
    Qubit Rna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4986 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit rna assay kit/product/Thermo Fisher
    Average 99 stars, based on 4986 article reviews
    Price from $9.99 to $1999.99
    qubit rna assay kit - by Bioz Stars, 2021-01
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    99
    Thermo Fisher qubit dsdna br assay kit
    Qubit Dsdna Br Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit dsdna br assay kit/product/Thermo Fisher
    Average 99 stars, based on 4279 article reviews
    Price from $9.99 to $1999.99
    qubit dsdna br assay kit - by Bioz Stars, 2021-01
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    99
    Thermo Fisher qubit rna hs assay kit
    Qubit Rna Hs Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit rna hs assay kit/product/Thermo Fisher
    Average 99 stars, based on 3196 article reviews
    Price from $9.99 to $1999.99
    qubit rna hs assay kit - by Bioz Stars, 2021-01
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    99
    Thermo Fisher qubit protein assay kit
    Qubit Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit protein assay kit/product/Thermo Fisher
    Average 99 stars, based on 3282 article reviews
    Price from $9.99 to $1999.99
    qubit protein assay kit - by Bioz Stars, 2021-01
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    92
    Thermo Fisher qubit fluorimeter
    cdPCR amplification plots of Fusarium <t>DNA</t> dilutions. The letters indicate the different fungal species: [ A ] = F. poae ; [ B ] = F. sporotrichioides ; [ C ] = F. graminearum ; [ D ] = F. avenaceum . The numbers indicate the dilutions factors: 1 means 0.25 ng of fungal DNA as <t>Qubit</t> quantified; 2 means sample 1 diluted 2.5 times; 3 means sample 2 diluted 10 times; 4 means sample 3 diluted 10 times. The blue dots are the PCR partitions resulted positive to amplification of the target; the yellow dots are the PCR partitions negative to amplification of the target.
    Qubit Fluorimeter, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit fluorimeter/product/Thermo Fisher
    Average 92 stars, based on 2147 article reviews
    Price from $9.99 to $1999.99
    qubit fluorimeter - by Bioz Stars, 2021-01
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    99
    Thermo Fisher qubit rna br assay kit
    cdPCR amplification plots of Fusarium <t>DNA</t> dilutions. The letters indicate the different fungal species: [ A ] = F. poae ; [ B ] = F. sporotrichioides ; [ C ] = F. graminearum ; [ D ] = F. avenaceum . The numbers indicate the dilutions factors: 1 means 0.25 ng of fungal DNA as <t>Qubit</t> quantified; 2 means sample 1 diluted 2.5 times; 3 means sample 2 diluted 10 times; 4 means sample 3 diluted 10 times. The blue dots are the PCR partitions resulted positive to amplification of the target; the yellow dots are the PCR partitions negative to amplification of the target.
    Qubit Rna Br Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1463 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit rna br assay kit/product/Thermo Fisher
    Average 99 stars, based on 1463 article reviews
    Price from $9.99 to $1999.99
    qubit rna br assay kit - by Bioz Stars, 2021-01
    99/100 stars
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    99
    Thermo Fisher qubit assay
    cdPCR amplification plots of Fusarium <t>DNA</t> dilutions. The letters indicate the different fungal species: [ A ] = F. poae ; [ B ] = F. sporotrichioides ; [ C ] = F. graminearum ; [ D ] = F. avenaceum . The numbers indicate the dilutions factors: 1 means 0.25 ng of fungal DNA as <t>Qubit</t> quantified; 2 means sample 1 diluted 2.5 times; 3 means sample 2 diluted 10 times; 4 means sample 3 diluted 10 times. The blue dots are the PCR partitions resulted positive to amplification of the target; the yellow dots are the PCR partitions negative to amplification of the target.
    Qubit Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1440 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit assay/product/Thermo Fisher
    Average 99 stars, based on 1440 article reviews
    Price from $9.99 to $1999.99
    qubit assay - by Bioz Stars, 2021-01
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    92
    Agilent technologies qubit
    Significant discrepancies in <t>DNA</t> quantification by NanoDrop and <t>Qubit.</t> A total of 100 ng of DNA based on NanoDrop (N, black bars) or Qubit (Q, grey bars) measurements was analyzed by electrophoresis on 0.8% agarose gel. Sample ID is indicated at the bottom. Lane L contains 200 ng of DNA as the reference for normalization. Densitometric analysis (bar chart) was performed by ImageJ software [20] . It is clear from the electrophoretic bands and their densitometric charts that NanoDrop overestimates DNA concentration.
    Qubit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit/product/Agilent technologies
    Average 92 stars, based on 1962 article reviews
    Price from $9.99 to $1999.99
    qubit - by Bioz Stars, 2021-01
    92/100 stars
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    99
    Thermo Fisher qubit 3 fluorometer
    Significant discrepancies in <t>DNA</t> quantification by NanoDrop and <t>Qubit.</t> A total of 100 ng of DNA based on NanoDrop (N, black bars) or Qubit (Q, grey bars) measurements was analyzed by electrophoresis on 0.8% agarose gel. Sample ID is indicated at the bottom. Lane L contains 200 ng of DNA as the reference for normalization. Densitometric analysis (bar chart) was performed by ImageJ software [20] . It is clear from the electrophoretic bands and their densitometric charts that NanoDrop overestimates DNA concentration.
    Qubit 3 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 508 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit 3 fluorometer/product/Thermo Fisher
    Average 99 stars, based on 508 article reviews
    Price from $9.99 to $1999.99
    qubit 3 fluorometer - by Bioz Stars, 2021-01
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    Image Search Results


    cdPCR amplification plots of Fusarium DNA dilutions. The letters indicate the different fungal species: [ A ] = F. poae ; [ B ] = F. sporotrichioides ; [ C ] = F. graminearum ; [ D ] = F. avenaceum . The numbers indicate the dilutions factors: 1 means 0.25 ng of fungal DNA as Qubit quantified; 2 means sample 1 diluted 2.5 times; 3 means sample 2 diluted 10 times; 4 means sample 3 diluted 10 times. The blue dots are the PCR partitions resulted positive to amplification of the target; the yellow dots are the PCR partitions negative to amplification of the target.

    Journal: Microorganisms

    Article Title: Moving from qPCR to Chip Digital PCR Assays for Tracking of some Fusarium species causing Fusarium Head Blight in Cereals

    doi: 10.3390/microorganisms8091307

    Figure Lengend Snippet: cdPCR amplification plots of Fusarium DNA dilutions. The letters indicate the different fungal species: [ A ] = F. poae ; [ B ] = F. sporotrichioides ; [ C ] = F. graminearum ; [ D ] = F. avenaceum . The numbers indicate the dilutions factors: 1 means 0.25 ng of fungal DNA as Qubit quantified; 2 means sample 1 diluted 2.5 times; 3 means sample 2 diluted 10 times; 4 means sample 3 diluted 10 times. The blue dots are the PCR partitions resulted positive to amplification of the target; the yellow dots are the PCR partitions negative to amplification of the target.

    Article Snippet: DNA concentrations were determined using Qubit® fluorimeter (Life Technologies™, Invitrogen, Monza, Italy).

    Techniques: Amplification, Polymerase Chain Reaction

    Significant discrepancies in DNA quantification by NanoDrop and Qubit. A total of 100 ng of DNA based on NanoDrop (N, black bars) or Qubit (Q, grey bars) measurements was analyzed by electrophoresis on 0.8% agarose gel. Sample ID is indicated at the bottom. Lane L contains 200 ng of DNA as the reference for normalization. Densitometric analysis (bar chart) was performed by ImageJ software [20] . It is clear from the electrophoretic bands and their densitometric charts that NanoDrop overestimates DNA concentration.

    Journal: PLoS ONE

    Article Title: DNA Qualification Workflow for Next Generation Sequencing of Histopathological Samples

    doi: 10.1371/journal.pone.0062692

    Figure Lengend Snippet: Significant discrepancies in DNA quantification by NanoDrop and Qubit. A total of 100 ng of DNA based on NanoDrop (N, black bars) or Qubit (Q, grey bars) measurements was analyzed by electrophoresis on 0.8% agarose gel. Sample ID is indicated at the bottom. Lane L contains 200 ng of DNA as the reference for normalization. Densitometric analysis (bar chart) was performed by ImageJ software [20] . It is clear from the electrophoretic bands and their densitometric charts that NanoDrop overestimates DNA concentration.

    Article Snippet: DNA qualification for next-generation-sequencing library construction To evaluate the effect of DNA quantification on NGS workflow, the construction of NGS libraries (Ion Torrent Ampliseq Cancer Panel) was performed using DNA from FFPE sample P. Forty nanograms of DNA, as calculated by NanoDrop or Qubit, were processed according to the manufacturers' protocol and the quality of the obtained library was evaluated by Agilent on-chip electrophoresis.

    Techniques: Electrophoresis, Agarose Gel Electrophoresis, Software, Concentration Assay

    DNA qualification for next-generation sequencing applications. Effect of low-quality DNA on next-generation sequencing (NGS) workflow. Three FFPE samples were tested for construction of NGS amplicon libraries (Ion Torrent Ampliseq Cancer Panel). Qubit: 40 ng of DNA according to Qubit measurement were processed using the Ampliseq library construction kit (multiplex PCR amplification of 191 DNA regions from 46 cancer-related genes). NanoDrop: absorption spectra of samples showed different degrees of organic contamination (230 nm spike, A260/A230 ratio). Agilent: quality and quantity of the obtained libraries were evaluated by Agilent high sensitivity assay on-chip electrophoresis, where the library is represented by the large band between 150 and 200 bp. Fragments test: histogram showing length and abundance of produced sequences. Sample FFPE 5 did not produce a good library due to high organic contamination; this is revealed by the remarkable spike at 230 nm that concurs to the low 260/230 ratio, and explains the faint electrophoretic band and the almost flat fragments test histogram.

    Journal: PLoS ONE

    Article Title: DNA Qualification Workflow for Next Generation Sequencing of Histopathological Samples

    doi: 10.1371/journal.pone.0062692

    Figure Lengend Snippet: DNA qualification for next-generation sequencing applications. Effect of low-quality DNA on next-generation sequencing (NGS) workflow. Three FFPE samples were tested for construction of NGS amplicon libraries (Ion Torrent Ampliseq Cancer Panel). Qubit: 40 ng of DNA according to Qubit measurement were processed using the Ampliseq library construction kit (multiplex PCR amplification of 191 DNA regions from 46 cancer-related genes). NanoDrop: absorption spectra of samples showed different degrees of organic contamination (230 nm spike, A260/A230 ratio). Agilent: quality and quantity of the obtained libraries were evaluated by Agilent high sensitivity assay on-chip electrophoresis, where the library is represented by the large band between 150 and 200 bp. Fragments test: histogram showing length and abundance of produced sequences. Sample FFPE 5 did not produce a good library due to high organic contamination; this is revealed by the remarkable spike at 230 nm that concurs to the low 260/230 ratio, and explains the faint electrophoretic band and the almost flat fragments test histogram.

    Article Snippet: DNA qualification for next-generation-sequencing library construction To evaluate the effect of DNA quantification on NGS workflow, the construction of NGS libraries (Ion Torrent Ampliseq Cancer Panel) was performed using DNA from FFPE sample P. Forty nanograms of DNA, as calculated by NanoDrop or Qubit, were processed according to the manufacturers' protocol and the quality of the obtained library was evaluated by Agilent on-chip electrophoresis.

    Techniques: Next-Generation Sequencing, Formalin-fixed Paraffin-Embedded, Amplification, Multiplex Assay, Polymerase Chain Reaction, Sensitive Assay, Chromatin Immunoprecipitation, Electrophoresis, Produced

    Influence of RNA contamination on DNA quantification. DNA quantifications (n = 5) by NanoDrop and Qubit in the presence of RNA contamination. A DNA sample with a concentration of 38 ng/µl was mixed with different volumes of total RNA at 33 ng/µl extracted from the same tissue sample to obtain the indicated ratios; bars and brackets indicate mean and 95% confidence interval; asterisks show measurements significantly different from pure DNA (* p

    Journal: PLoS ONE

    Article Title: DNA Qualification Workflow for Next Generation Sequencing of Histopathological Samples

    doi: 10.1371/journal.pone.0062692

    Figure Lengend Snippet: Influence of RNA contamination on DNA quantification. DNA quantifications (n = 5) by NanoDrop and Qubit in the presence of RNA contamination. A DNA sample with a concentration of 38 ng/µl was mixed with different volumes of total RNA at 33 ng/µl extracted from the same tissue sample to obtain the indicated ratios; bars and brackets indicate mean and 95% confidence interval; asterisks show measurements significantly different from pure DNA (* p

    Article Snippet: DNA qualification for next-generation-sequencing library construction To evaluate the effect of DNA quantification on NGS workflow, the construction of NGS libraries (Ion Torrent Ampliseq Cancer Panel) was performed using DNA from FFPE sample P. Forty nanograms of DNA, as calculated by NanoDrop or Qubit, were processed according to the manufacturers' protocol and the quality of the obtained library was evaluated by Agilent on-chip electrophoresis.

    Techniques: Concentration Assay

    Cross-validation of DNA samples quantification by qPCR. Bland-Altman plots for inter-technology (NanoDrop or Qubit vs. qPCR) comparison of all samples (A), and according to the different sample sources, as indicated (B, C). A) Qubit measurements show high correlation (mean measured/expected ratio = 0.92; SD = 0.69; Wilcoxon signed rank test p = 0.07) with the measurements obtained by qPCR (x-axis), whereas NanoDrop measurements tend to overestimate samples concentration (mean measured/expected ratio = 3.8; SD = 6.4; Wilcoxon signed rank test p

    Journal: PLoS ONE

    Article Title: DNA Qualification Workflow for Next Generation Sequencing of Histopathological Samples

    doi: 10.1371/journal.pone.0062692

    Figure Lengend Snippet: Cross-validation of DNA samples quantification by qPCR. Bland-Altman plots for inter-technology (NanoDrop or Qubit vs. qPCR) comparison of all samples (A), and according to the different sample sources, as indicated (B, C). A) Qubit measurements show high correlation (mean measured/expected ratio = 0.92; SD = 0.69; Wilcoxon signed rank test p = 0.07) with the measurements obtained by qPCR (x-axis), whereas NanoDrop measurements tend to overestimate samples concentration (mean measured/expected ratio = 3.8; SD = 6.4; Wilcoxon signed rank test p

    Article Snippet: DNA qualification for next-generation-sequencing library construction To evaluate the effect of DNA quantification on NGS workflow, the construction of NGS libraries (Ion Torrent Ampliseq Cancer Panel) was performed using DNA from FFPE sample P. Forty nanograms of DNA, as calculated by NanoDrop or Qubit, were processed according to the manufacturers' protocol and the quality of the obtained library was evaluated by Agilent on-chip electrophoresis.

    Techniques: Real-time Polymerase Chain Reaction, Concentration Assay

    Intra- and inter-method accuracy and precision. Distribution of DNA sample concentration (dispersion chart) was estimated by both NanoDrop (black) and Qubit (gray) on repeated (n = 20) measurements of two commercial human genomic DNA preparations (Sample L 200 ng/µl; Sample G 5 ng/µl). For both samples, NanoDrop overestimated the DNA concentration (+8.8% for L and +24.0% for G, p

    Journal: PLoS ONE

    Article Title: DNA Qualification Workflow for Next Generation Sequencing of Histopathological Samples

    doi: 10.1371/journal.pone.0062692

    Figure Lengend Snippet: Intra- and inter-method accuracy and precision. Distribution of DNA sample concentration (dispersion chart) was estimated by both NanoDrop (black) and Qubit (gray) on repeated (n = 20) measurements of two commercial human genomic DNA preparations (Sample L 200 ng/µl; Sample G 5 ng/µl). For both samples, NanoDrop overestimated the DNA concentration (+8.8% for L and +24.0% for G, p

    Article Snippet: DNA qualification for next-generation-sequencing library construction To evaluate the effect of DNA quantification on NGS workflow, the construction of NGS libraries (Ion Torrent Ampliseq Cancer Panel) was performed using DNA from FFPE sample P. Forty nanograms of DNA, as calculated by NanoDrop or Qubit, were processed according to the manufacturers' protocol and the quality of the obtained library was evaluated by Agilent on-chip electrophoresis.

    Techniques: Concentration Assay