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  • 99
    Thermo Fisher qubit assay tubes
    FFPE DNA characterization by QFI-PCR and fluorescence-based assays from 165 tumor DNA samples. (A) Distribution of FFPE DNA quantification using QFI-PCR and the fluorescence-based <t>Qubit</t> <t>dsDNA</t> HS assay from 5 ng bulk DNA input as determined by NanoDrop spectrophotometry. A total of 27 samples were undetected by fluorescence assay (
    Qubit Assay Tubes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit assay tubes/product/Thermo Fisher
    Average 99 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
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    94
    Thermo Fisher qubit
    Average <t>Nanodrop:Qubit</t> ratio for each laboratory as well as the average and median ratio for the entire cohort.
    Qubit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 17630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit/product/Thermo Fisher
    Average 94 stars, based on 17630 article reviews
    Price from $9.99 to $1999.99
    qubit - by Bioz Stars, 2020-08
    94/100 stars
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    85
    Thermo Fisher qubit it
    Average <t>Nanodrop:Qubit</t> ratio for each laboratory as well as the average and median ratio for the entire cohort.
    Qubit It, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit it/product/Thermo Fisher
    Average 85 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    qubit it - by Bioz Stars, 2020-08
    85/100 stars
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    99
    Thermo Fisher qubit 3 0 fluorometer
    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p
    Qubit 3 0 Fluorometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit 3 0 fluorometer/product/Thermo Fisher
    Average 99 stars, based on 5117 article reviews
    Price from $9.99 to $1999.99
    qubit 3 0 fluorometer - by Bioz Stars, 2020-08
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    91
    Fisher Scientific qubit
    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p
    Qubit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 91/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit/product/Fisher Scientific
    Average 91 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    qubit - by Bioz Stars, 2020-08
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    93
    Agilent technologies qubit
    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p
    Qubit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit/product/Agilent technologies
    Average 93 stars, based on 1962 article reviews
    Price from $9.99 to $1999.99
    qubit - by Bioz Stars, 2020-08
    93/100 stars
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    97
    Thermo Fisher qubit ssdna kit
    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p
    Qubit Ssdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit ssdna kit/product/Thermo Fisher
    Average 97 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    qubit ssdna kit - by Bioz Stars, 2020-08
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    99
    Thermo Fisher qubit assay kit
    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p
    Qubit Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit assay kit/product/Thermo Fisher
    Average 99 stars, based on 273 article reviews
    Price from $9.99 to $1999.99
    qubit assay kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    FFPE DNA characterization by QFI-PCR and fluorescence-based assays from 165 tumor DNA samples. (A) Distribution of FFPE DNA quantification using QFI-PCR and the fluorescence-based Qubit dsDNA HS assay from 5 ng bulk DNA input as determined by NanoDrop spectrophotometry. A total of 27 samples were undetected by fluorescence assay (

    Journal: Genome Medicine

    Article Title: Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies

    doi: 10.1186/gm481

    Figure Lengend Snippet: FFPE DNA characterization by QFI-PCR and fluorescence-based assays from 165 tumor DNA samples. (A) Distribution of FFPE DNA quantification using QFI-PCR and the fluorescence-based Qubit dsDNA HS assay from 5 ng bulk DNA input as determined by NanoDrop spectrophotometry. A total of 27 samples were undetected by fluorescence assay (

    Article Snippet: Briefly, 1 μl of DNA sample at 5 ng/μl was diluted 200-fold in Qubit dsDNA HS buffer in clear plastic Qubit Assay Tubes (catalogue number Q32856, Life Technologies) and measured on the fluorometer.

    Techniques: Formalin-fixed Paraffin-Embedded, Polymerase Chain Reaction, Fluorescence, Spectrophotometry

    Average Nanodrop:Qubit ratio for each laboratory as well as the average and median ratio for the entire cohort.

    Journal: Journal of Clinical Pathology

    Article Title: Variation in pre-PCR processing of FFPE samples leads to discrepancies in BRAF and EGFR mutation detection: a diagnostic RING trial

    doi: 10.1136/jclinpath-2014-202644

    Figure Lengend Snippet: Average Nanodrop:Qubit ratio for each laboratory as well as the average and median ratio for the entire cohort.

    Article Snippet: Comparing Qubit versus Nanodrop measurements for identical samples, represented as the Nanodrop:Qubit (N/Q) ratio, demonstrates median Nanodrop readings were 5.1-fold higher than Qubit measurements for the same samples ( ).

    Techniques:

    Flowchart for the All-in-One sequencing (AIO-seq) method. a The libraries were prepared using Tn5 transposase. b The process of mechanical fragmentation was used to prepare the libraries. c In the traditional protocol, the size selection, and quantification were processed using a one sample one tube method. d With the AIO-seq method, the library analyzed by the Agilent 2100 Bioanalyzer will give the fragment distribution pattern and the ratio of the target region (between the two blue lines) to the total library. e The concentration of the total library could be obtained by Qubit™ 4.0 Fluorometer. f The target region concentrations (TRC) were calculated within each library by multiplying the proportion of the target region from ( d ) and the total library concentration from ( e ). g Mixing the libraries in one tube according to the calculated TRC and their expected yields of the sequence data. h – i One size selection by Sage HT. j Quantification of the selected fragment by qPCR and sequencing

    Journal: Plant Methods

    Article Title: All-in-one sequencing: an improved library preparation method for cost-effective and high-throughput next-generation sequencing

    doi: 10.1186/s13007-020-00615-3

    Figure Lengend Snippet: Flowchart for the All-in-One sequencing (AIO-seq) method. a The libraries were prepared using Tn5 transposase. b The process of mechanical fragmentation was used to prepare the libraries. c In the traditional protocol, the size selection, and quantification were processed using a one sample one tube method. d With the AIO-seq method, the library analyzed by the Agilent 2100 Bioanalyzer will give the fragment distribution pattern and the ratio of the target region (between the two blue lines) to the total library. e The concentration of the total library could be obtained by Qubit™ 4.0 Fluorometer. f The target region concentrations (TRC) were calculated within each library by multiplying the proportion of the target region from ( d ) and the total library concentration from ( e ). g Mixing the libraries in one tube according to the calculated TRC and their expected yields of the sequence data. h – i One size selection by Sage HT. j Quantification of the selected fragment by qPCR and sequencing

    Article Snippet: For the RIL samples, the PCR products were mixed directly without the Agilent 2100 Bioanalyzer and Qubit assay, followed by purification with 1.8 × VAHTS™ DNA Clean Beads.

    Techniques: Sequencing, Selection, Concentration Assay, Real-time Polymerase Chain Reaction

    DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER

    Journal: PLoS ONE

    Article Title: Bisulfite Conversion of DNA: Performance Comparison of Different Kits and Methylation Quantitation of Epigenetic Biomarkers that Have the Potential to Be Used in Non-Invasive Prenatal Testing

    doi: 10.1371/journal.pone.0135058

    Figure Lengend Snippet: DNA degradation. DNA recovery after bisulfite treatment was determined with the Qubit fluorometer. DNA recovery with the Epigentek kit was 33.2% ± 3.4%, with the Promega kit was 52% ± 3%, with the Diagenode kit was 55% ± 2.6% and with the Qiagen kit was 50.2% ± 2%. The BisulFlash DNA Modification kit was shown to have a significantly decreased average yield with respect to all other kits (FWER

    Article Snippet: DNA yield and degradation In order to compare the DNA yield and hence, the degradation effect of each of the four different kits we used the Qubit fluorometer (Invitrogen) to determine the bisulfite-treated DNA concentration.

    Techniques: Modification

    a  3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [  8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth.  b  Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5  ×  10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [  40 ].  c  Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls

    Journal: BMC Cancer

    Article Title: Hypoxic resistance of KRAS mutant tumor cells to 3-Bromopyruvate is counteracted by Prima-1 and reversed by N-acetylcysteine

    doi: 10.1186/s12885-016-2930-9

    Figure Lengend Snippet: a 3-BrPA potentiates Prima-1 toxicity against A549 cells in 5 mM glucose. A549 cells (4X10 3 ) were seeded in tissue culture 96 well plates in complete medium containing 20 mM glucose and 10% fetal bovine serum, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum for 48 h. Relative proliferation /toxicity was assayed fluorometrically in octuplicate by the Alamar Blue method by quantitating the conversion of resazurin to fluorescent resorufin [ 8 ]. This revealed that 50 μM Prima-1 cooperated with 3-BrPA rather than with CHC to suppress A549 cell growth. b Prima-1 decreases SLC2A1-GLUT1 in A549 cells. Sparse cells were seeded in 5 cm tissue culture plates (5  ×  10 5 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 18 h, then washed 3X with PBS and treated as indicated in each case, in medium supplemented with physiological 5 mM glucose, 2 mM glutamine and 5% dialyzed serum whenever indicated (+) for 24 h. After RNA extraction with TRIZOL and quantification in a Qubit® 2.0 Fluorometer, cDNAs were prepared for end-point PCR analysis as indicated under Methods.essentially similar results were obtained in cells treated with Prima-1 in 5 mM glucose (not shown). Cells treated in parallel with those used for RNA analysis were used for GLUT1 protein immune blot [ 40 ]. c Prima-1 activates p21CDKN1A gene expression in A549 cells in 5 mM glucose. qPCR was used to determine relative expression of the p21CDK1N1 gene in control and treated cells, after RNA extraction, cDNA preparation and qPCR, as indicated under Methods. *denotes significance between treated cells relative to controls

    Article Snippet: Real-time and end-point RT-PCR Cells were seeded in 5 cm-well plates (3 × 105 cells per plate) in complete Dulbecco’s medium containing 20 mM glucose supplemented with 10% serum for 24 h. Cells were washed 3X with PBS and treated as indicated in medium supplemented with physiological 5 mM glucose and 5% dialyzed serum for 24 h. RNA extraction was performed using TRIZOL® (Life Technologies, Cat # 15596–026) and quantification was determined using a Qubit® 2.0 Fluorometer (Life Technologies, Cat #Q32866) with a Qubit™ RNA Assay Kit (Life Technologies, Cat # Q32852).

    Techniques: RNA Extraction, Polymerase Chain Reaction, Expressing, Real-time Polymerase Chain Reaction

    Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) .  Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p

    Journal: Cancers

    Article Title: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics

    doi: 10.3390/cancers11111691

    Figure Lengend Snippet: Comparison of the three manual DNA extraction systems (MM, PC and GR). Mean of DNA concentration (ng/μL) and total DNA (ng) as measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ) . Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) and DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. * p

    Article Snippet: Library quantification was assessed using a Qubit® 3.0 Fluorometer (Thermo Fisher, Waltham, MA, USA) using the Qubit dsDNA HS (high sensitivity) assay kit (Life Technology, Thermo Fisher Scientific).

    Techniques: DNA Extraction, Concentration Assay, Spectrophotometry

    Comparison of two automatic DNA extraction systems (OMNIA Prima and King Fisher Duo). DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ). DNA (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. *  p

    Journal: Cancers

    Article Title: Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics

    doi: 10.3390/cancers11111691

    Figure Lengend Snippet: Comparison of two automatic DNA extraction systems (OMNIA Prima and King Fisher Duo). DNA concentration (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( A ) and Qubit 3.0 fluorometer ( B ). DNA (ng/μL) measured by the NanoDrop D-1000 spectrophotometer ( C ) and Qubit 3.0 fluorometer ( D ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. Total DNA amount (ng) measured by the NanoDrop D-1000 spectrophotometer ( E ) and Qubit 3.0 fluorometer ( F ) for the 24N, 24T, 48N, 48T, 72N, and 72T samples. * p

    Article Snippet: Library quantification was assessed using a Qubit® 3.0 Fluorometer (Thermo Fisher, Waltham, MA, USA) using the Qubit dsDNA HS (high sensitivity) assay kit (Life Technology, Thermo Fisher Scientific).

    Techniques: DNA Extraction, Concentration Assay, Spectrophotometry