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All (84)
Finkenbeiner
Chemglass Life Sciences
Ted Pella
Technical Glass
HELLMA
SPI Supplies
AdValue Tech
Quartz Scientific
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About 262 products / 1,104 snippets / 15 images

88 / 100
Bioz Stars
Quartz: Quartz slides
Chemglass Life Sciences  
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Techniques
 
Article Snippets
"Self-assembled monolayers on quartz: Quartz slides (fused silica - Chemglass) were cleaned prior to silanization by boiling in acetone and .."  (see more)
91 / 100
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Quartz slide
Chemglass Life Sciences  
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Techniques
 
Article Snippets
"The measurements were conducted on a 25 mm × 25 mm quartz slide (Chemglass) placed in the glass cell."  (see more)
11+  more images
(A) Schematic diagram of the prism-based single-molecule TIRF <t>microscope</t> setup. The optics components include mirrors (M1–6), band-pass filters (F1–4), dichroic mirrors (D1–2) and converging lenses (L1–2). The excitation laser beam was focused on the flow cell through the prism. M2 was positioned with a six-axis mount to adjust the incident angle of the laser beam. The fluorophore labeled sample molecules were illuminated by the evanescent wave created by the total internal reflection at the quartz-water interface. The fluorescence emission was collected by the objective and split into the donor and acceptor channels by the DualView and then imaged on CCD chip side by side. (B) Microscope flow cell design and slide surface functionalization. The flow cell was made by attaching the glass coverslips to the quartz slide using parafilm with cut flow channels. The quartz slide surface is functionalized with PEG and biotin-PEG. Biotinylated sample molecules were tethered on the slide surface through the biotin-streptavidin-biotin interaction. The mononucleosome and a dinucleosome array are made using the DNA constructs as shown in Fig. 1D
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Schematic of a prism-based total internal reflection <t>microscope</t> for single-molecule spectroscopy. A circularly polarized 532-nm laser and a linear polarized 635-nm laser are combined using a dichroic mirror and routed to a quartz prism on the microscope stage. Mechanical shutters control the excitation wavelength. The fluorescence emission is split by color into two images using a dichroic mirror, which are passed through additional optical elements to isolate donor and acceptor signals. The two replicate images are collected by an EMCCD camera and are processed using MATLAB to identify single molecules containing a donor and acceptor fluorophore.
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Schematic diagram of prism-based single-molecular total internal reflection fluorescence (TIRF) microscopy and representative TIRF field-of-view image with the donor and acceptor channels. A , schematic of single-molecule TIRF imaging of GCGR in detergent micelle labeled with donor and acceptor fluorophores. The excitation beam reaches the slide-solution interface and creates an evanescent wave that excites immobilized molecules in an aqueous solution. The emission from the donor and acceptor are collected through an inverted <t>microscope</t> objective and passed through a dual-view splitter, where the donor ( green ) and acceptor ( red ) emissions are physically separated. Finally, the two emission signals are detected side-by-side by an electron-multiplied back-illuminated CCD camera. The expanded view showing Alexa Fluor 555- ( green sphere ) and Alexa Fluor 647- ( red sphere ) labeled GCGR ( orange cylinders ) in a detergent micelle ( blue ) immobilized to a microscope slide using biotin ( orange circles ), streptavidin ( gray semicircles ) and biotinylated anti-FLAG antibody ( green Y shape ). The microscope slides were passivated with polyethylene glycol ( black wavy lines ) to decrease the nonspecific adsorption of the labeled micelles. A 532 nm laser was used to excite the Alexa Fluor 555 fluorophore. This image was prepared with BioRender. B , a representative TIRF field-of-view image with the donor channel on the left and the acceptor channel on the right . Scale bar, 5 μm. ECD, extracellular domain; FRET, fluorescence resonance energy transfer; GCGR, glucagon receptor.
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smFRET analyses reveal random diffusion of Rad6/Rad18 complexes on RPA filaments. ( A ) Experimental schematic to monitor translocation of a Rad6/Rad18 complex along RPA filaments via smFRET. The BioCy3P/T-70ss DNA substrate is immobilized on a <t>microscope</t> slide surface via biotin/streptavidin conjugation and the ssDNA region is saturated with two RPA molecules. Cy5-Rad6/Rad18 is injected and smFRET is monitored over time. ( B ) An example of a time trajectory shows the fluctuating smFRET efficiency during τ on , indicating that a Rad6/Rad18 complex translocates along an RPA filament (right). ( C ) Collective smFRET efficiency time trajectories (n = 88) synchronized at the starting point of smFRET events (i.e. starting points of the τon windows shown in panel B ) were overlaid ( Left ). The average smFRET efficiency (indicated) is calculated from the histogram of the smFRET efficiencies observed during the τ on windows ( Right ).
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90 / 100
Bioz Stars
Quartz slides
Thermo Fisher Scientific / Invitrogen  
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Techniques
 
Article Snippets
"After streptavidin (0.2 mg/mL; Invitrogen) coating of polymer-coated quartz slides ( 39 ) for 5 min, the EC (400 pM) was immobilized .."  (see more)
92 / 100
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Quartz slides
AdValue Technology  
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Techniques
 
Article Snippets
"Quartz slides were purchased from Advalue Technologies."  (see more)
92 / 100
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Quartz slide
Fisher Scientific  
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Techniques
 
Article Snippets
".. fluorescent beads solution (Molecular Probe, Fisher Scientific) was deposited on a quartz slide (Fisher Scientific) and covered with a 25 mm glass (Fisher) coverslip pretreated with .."  (see more)
91 / 100
Bioz Stars
Quartz slides
SPI Supplies  
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Techniques
 
Article Snippets
".. doped with Boron, p-type, thickness of 500-550 μm, <100> orientation) and quartz slides (SPI supplies, 1 mm of thickness)."  (see more)
90 / 100
Bioz Stars
Quartz slide
Thermo Fisher Scientific / Molecular Probes  
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Techniques
 
Article Snippets
"Labeled-Protein Deposition 700 μ L of 10 μ M FITC-HSA ( L = 9, Sigma-Aldrich) or Texas Red-BSA ( L = 3.5, Molecular Probes; where L is the molar ratio of fluorophore to protein molecule) solution in 0.01× PBS was deposited on each quartz slide (45 × 12.5 mm, half .."  (see more)
80 / 100
Bioz Stars
Quartz slides
MTI  
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Techniques
 
Article Snippets
"Quartz slides (1 by 1 cm, one-side polished, MTI Corp) are pre-cleaned by sonicating in a sequence of chloroform, acetone, .."  (see more)
91 / 100
Bioz Stars
Quartz slides
Finkenbeiner  
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Techniques
 
Article Snippets
"Single-molecule fluorescence experiments were carried out on quartz slides (Finkenbeiner)."  (see more)
85 / 100
Bioz Stars
Quartz slides
Agar Scientific  
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Techniques
 
Article Snippets
"Quartz slides (50 mm × 25 mm × 1 mm) were supplied by Agar Scientific (Stansted, U.K.) and coverslips (20 .."  (see more)
91 / 100
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Quartz slides
Ted Pella  
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Techniques
 
Article Snippets
"Substrates for layer-by-layer superlattice assembly were prepared using quartz slides (Ted Pella) using DNA that was synthesized and purified as described above."  (see more)
91 / 100
Bioz Stars
Quartz slides
Technical Glass  
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Techniques
 
Article Snippets
".. created by inserting polyethylene spacers between two clean DUV transmitting fused quartz slides (Grade GE124, Technical Glass Products, WA, USA), placed horizontally on a metal stand."  (see more)
91 / 100
Bioz Stars
Quartz slides
Danaher / Cytiva  
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Techniques
 
Article Snippets
"Abstract: Electroless gold island thin films are formed by galvanic replacement of silver reduced onto a tin-sensitized silica surface.. A novel approach to create nanoparticle ensembles with tunable particle dimensions, densities, and distri ..."  (see more)