quantitative pcr Search Results


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    For use with the GeneRead Library Quant System Kit contents Two 2 tubes each containing 1 35 ml of 2x solution sufficient for 100 standard 25 l reactions Benefits Specific
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    384 well qPCR plate PP Full Skirt 5 bags of 10 plate
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    79
    Roche quantitative pcr quantitative polymerase chain reaction qpcr
    Quantitative PCR results of the 3 confirmed <t>CNV</t> differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female
    Quantitative Pcr Quantitative Polymerase Chain Reaction Qpcr, supplied by Roche, used in various techniques. Bioz Stars score: 79/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad quantitative polymerase chain reaction qpcr quantitative pcr
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Qpcr Quantitative Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche quantitative pcr analysis a lightcyler 96 quantitative polymerase chain reaction qpcr system
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Pcr Analysis A Lightcyler 96 Quantitative Polymerase Chain Reaction Qpcr System, supplied by Roche, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1508 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction qpcr
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    Quantitative Polymerase Chain Reaction Qpcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG fluorescent quantitative polymerase chain reaction pcr
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    Fluorescent Quantitative Polymerase Chain Reaction Pcr, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG fluorescence quantitative polymerase chain reaction pcr
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Fluorescence Quantitative Polymerase Chain Reaction Pcr, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 82/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler quantitative polymerase chain reaction pcr
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    Lightcycler Quantitative Polymerase Chain Reaction Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co quantitative polymerase chain reaction qpcr kit
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    Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene quantitative polymerase chain reaction qpcr machine
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    Quantitative Polymerase Chain Reaction Qpcr Machine, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction qpcr kit
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    Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems quantitative polymerase chain reaction qpcr reagent
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    Quantitative Polymerase Chain Reaction Qpcr Reagent, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr kits
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    Quantitative Polymerase Chain Reaction Qpcr Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore quantitative polymerase chain reaction qpcr primers
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    Quantitative Polymerase Chain Reaction Qpcr Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad myiq quantitative polymerase chain reaction pcr machine
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Myiq Quantitative Polymerase Chain Reaction Pcr Machine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fluorescence quantitative polymerase chain reaction pcr kit
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Fluorescence Quantitative Polymerase Chain Reaction Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Kehua fluorescence quantitative polymerase chain reaction fq pcr
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    Fluorescence Quantitative Polymerase Chain Reaction Fq Pcr, supplied by Shanghai Kehua, used in various techniques. Bioz Stars score: 75/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega fluorescent quantitative polymerase chain reaction pcr kit
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    Fluorescent Quantitative Polymerase Chain Reaction Pcr Kit, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7900ht quantitative polymerase chain reaction pcr instrument
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    7900ht Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fluorescence quantitative polymerase chain reaction pcr instrument
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Fluorescence Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TIB MOLBIOL quantitative polymerase chain reacton qpcr primers
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    Quantitative Polymerase Chain Reacton Qpcr Primers, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SuperArray Bioscience Corporation superarray quantitative polymerase chain reaction pcr assay
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    Superarray Quantitative Polymerase Chain Reaction Pcr Assay, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative real time polymerase chain reaction pcr quantitative real time polymerase chain reaction
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Real Time Polymerase Chain Reaction Pcr Quantitative Real Time Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene mx3005p quantitative polymerase chain reaction qpcr system
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Mx3005p Quantitative Polymerase Chain Reaction Qpcr System, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr reagents
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Polymerase Chain Reaction Qpcr Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG quantitative polymerase chain reaction qpcr detection system
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Polymerase Chain Reaction Qpcr Detection System, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 82/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction qpcr pcr amplification
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Polymerase Chain Reaction Qpcr Pcr Amplification, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Capital Biosciences evagreen quantitative polymerase chain reaction qpcr kits
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Evagreen Quantitative Polymerase Chain Reaction Qpcr Kits, supplied by Capital Biosciences, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr trizol reagent
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    Quantitative Polymerase Chain Reaction Qpcr Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research quantitative rt pcr quantitative polymerase chain reaction
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    Quantitative Rt Pcr Quantitative Polymerase Chain Reaction, supplied by MJ Research, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

    Journal: Molecular Syndromology

    Article Title: Differences in Copy Number Variation between Discordant Monozygotic Twins as a Model for Exploring Chromosomal Mosaicism in Congenital Heart Defects

    doi: 10.1159/000335284

    Figure Lengend Snippet: Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

    Article Snippet: To confirm the presence of CNV differences, quantitative polymerase chain reaction (qPCR) was performed on DNA from both twin members and 3 unrelated controls (2 males and 1 female) using the LightCycler® 480 Real-Time PCR system (Roche Applied Science, Belgium, ), following the manufacturer's instructions.

    Techniques: Real-time Polymerase Chain Reaction

    ( A ) qPCR data of Bacterial and Archaeal 16S copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis. ( B ) qPCR data of Bacterial and Archaeal 16S rRNA transcripts copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis.

    Journal: FEMS Microbiology Ecology

    Article Title: Cold adaptation and replicable microbial community development during long-term low-temperature anaerobic digestion treatment of synthetic sewage

    doi: 10.1093/femsec/fiy095

    Figure Lengend Snippet: ( A ) qPCR data of Bacterial and Archaeal 16S copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis. ( B ) qPCR data of Bacterial and Archaeal 16S rRNA transcripts copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis.

    Article Snippet: Quantitative-polymerase chain reaction Quantitative polymerase chain reaction (qPCR) was carried out for Archaeal and Bacterial domains using DNA and cDNA generated from granular biomass sampled from R1 and R2 and the fixed-film filter as described above. qPCR was performed using a LightCycler 480 (Roche, Manheim, Germany).

    Techniques: Real-time Polymerase Chain Reaction

    16S rRNA gene qPCR of DNA prepared from unfractionated BAL samples. The number of copies of bacterial 16S rRNA genes per 5's multiple comparisons test.

    Journal: PLoS ONE

    Article Title: Changes in the Lung Microbiome following Lung Transplantation Include the Emergence of Two Distinct Pseudomonas Species with Distinct Clinical Associations

    doi: 10.1371/journal.pone.0097214

    Figure Lengend Snippet: 16S rRNA gene qPCR of DNA prepared from unfractionated BAL samples. The number of copies of bacterial 16S rRNA genes per 5's multiple comparisons test.

    Article Snippet: Quantitative Polymerase Chain Reaction (qPCR) Quantification of bacterial 16S rDNA was performed by real-time PCR utilizing TaqMan hydrolysis probes on a Roche 480 LightCycler.

    Techniques: Real-time Polymerase Chain Reaction

    Fold decrements of template DNA of the β-globin and rhodopsin genes after MNase digestion of chromatin samples quantified by qPCR. Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .

    Journal: Nucleic Acids Research

    Article Title: Linker histone partial phosphorylation: effects on secondary structure and chromatin condensation

    doi: 10.1093/nar/gkv304

    Figure Lengend Snippet: Fold decrements of template DNA of the β-globin and rhodopsin genes after MNase digestion of chromatin samples quantified by qPCR. Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .

    Article Snippet: Purified DNA was used as a template for the amplification of β-globin and rhodopsin genes by Quantitative Polymerase Chain Reaction (qPCR), following MIQE guidelines, in a C1000TM thermal cycler (BioRad).

    Techniques: Real-time Polymerase Chain Reaction

    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) mRNA expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P

    Journal: Molecular Medicine Reports

    Article Title: Potential application of injectable chitosan hydrogel treated with siRNA in chronic rhinosinusitis therapy

    doi: 10.3892/mmr.2015.4237

    Figure Lengend Snippet: (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) mRNA expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P

    Article Snippet: The mRNA expression of target VEGF was analyzed using quantitative polymerase chain reaction (qPCR) with an SYBR Premix ExTM Taq II kit (Takara Bio, Inc.).

    Techniques: Fluorescence, Microscopy, Expressing, Real-time Polymerase Chain Reaction

    Expression levels of cullin 3 protein and mRNA and proteins of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade in the kidneys of Cullin 3 G(−1)A/+ knock-in mice. (A) Immunoblots of proteins of the WNK–OSR1/SPAK–NCC signaling cascade in the kidneys of wild-type (WT) and cullin 3 ( Cul3 ) G(−1)A/+ heterozygous knock-in mice. (B) Densitometry analysis. Values are expressed as a ratio of the average signal in WT mice. Cul3 expression levels in Cul3 G(−1)A/+ heterozygous knock-in mice were approximately half that of WT mice. There were no significant differences in proteins expression levels of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade between Cul3 G(−1)A/+ heterozygous knock-in and WT mice. (C) Quantitative polymerase chain reaction (PCR) analysis of Cul3 mRNA levels. SYBR Green quantitative PCR was used to quantify mRNA levels in the kidneys of WT mice ( n =7) and Cul3 G(−1)A/+ mice ( n =7). WT: wild type. G(−1)A: Cul3 G(−1)A/+ . * P

    Journal: Biology Open

    Article Title: Generation and analysis of knock-in mice carrying pseudohypoaldosteronism type II-causing mutations in the cullin 3 gene

    doi: 10.1242/bio.013276

    Figure Lengend Snippet: Expression levels of cullin 3 protein and mRNA and proteins of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade in the kidneys of Cullin 3 G(−1)A/+ knock-in mice. (A) Immunoblots of proteins of the WNK–OSR1/SPAK–NCC signaling cascade in the kidneys of wild-type (WT) and cullin 3 ( Cul3 ) G(−1)A/+ heterozygous knock-in mice. (B) Densitometry analysis. Values are expressed as a ratio of the average signal in WT mice. Cul3 expression levels in Cul3 G(−1)A/+ heterozygous knock-in mice were approximately half that of WT mice. There were no significant differences in proteins expression levels of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade between Cul3 G(−1)A/+ heterozygous knock-in and WT mice. (C) Quantitative polymerase chain reaction (PCR) analysis of Cul3 mRNA levels. SYBR Green quantitative PCR was used to quantify mRNA levels in the kidneys of WT mice ( n =7) and Cul3 G(−1)A/+ mice ( n =7). WT: wild type. G(−1)A: Cul3 G(−1)A/+ . * P

    Article Snippet: Quantitative polymerase chain reaction Quantitative PCR was performed using SYBR Premix Ex Taq™ II (Takara Bio Inc.) and primers specific for Cul3 , using the forward primer F4 and the reverse primer R5 (5′-AGCCCTGGATATAGTCAACAGG-3′).

    Techniques: Expressing, Knock-In, Mouse Assay, Western Blot, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, SYBR Green Assay

    Expression levels of cullin 3 protein and mRNA and proteins of the WNK-OSR1/SPAK-NCC phosphorylation signaling cascade in the kidneys of cullin 3 T(−6)G/T(−6)G knock-in mice. (A) Immunoblots of proteins of the WNK–OSR1/SPAK–NCC signaling cascade in the kidneys of wild-type (WT) and cullin 3 ( Cul3 ) T( − 6)G/T( − 6)G homozygous knock-in mice. (B) Densitometry analysis. Values are expressed as the ratio of the average signal in WT mice. The level of Cul3 protein expression in Cul3 T( − 6)G/T( − 6)G mice was three-quarters that of WT mice. There were no significant differences in proteins expression levels of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade between Cul3 T( − 6)G/T( − 6)G and WT mice. (C) Quantitative polymerase chain reaction (PCR) analysis of Cul3 mRNA levels. SYBR Green quantitative PCR was used to quantify mRNA levels in the kidneys of WT mice ( n =7) and Cul3 T( − 6)G/T( − 6)G mice ( n =9). WT: wild-type mice. T(−6)G: Cul3 T( − 6)G/T( − 6)G mice. * P

    Journal: Biology Open

    Article Title: Generation and analysis of knock-in mice carrying pseudohypoaldosteronism type II-causing mutations in the cullin 3 gene

    doi: 10.1242/bio.013276

    Figure Lengend Snippet: Expression levels of cullin 3 protein and mRNA and proteins of the WNK-OSR1/SPAK-NCC phosphorylation signaling cascade in the kidneys of cullin 3 T(−6)G/T(−6)G knock-in mice. (A) Immunoblots of proteins of the WNK–OSR1/SPAK–NCC signaling cascade in the kidneys of wild-type (WT) and cullin 3 ( Cul3 ) T( − 6)G/T( − 6)G homozygous knock-in mice. (B) Densitometry analysis. Values are expressed as the ratio of the average signal in WT mice. The level of Cul3 protein expression in Cul3 T( − 6)G/T( − 6)G mice was three-quarters that of WT mice. There were no significant differences in proteins expression levels of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade between Cul3 T( − 6)G/T( − 6)G and WT mice. (C) Quantitative polymerase chain reaction (PCR) analysis of Cul3 mRNA levels. SYBR Green quantitative PCR was used to quantify mRNA levels in the kidneys of WT mice ( n =7) and Cul3 T( − 6)G/T( − 6)G mice ( n =9). WT: wild-type mice. T(−6)G: Cul3 T( − 6)G/T( − 6)G mice. * P

    Article Snippet: Quantitative polymerase chain reaction Quantitative PCR was performed using SYBR Premix Ex Taq™ II (Takara Bio Inc.) and primers specific for Cul3 , using the forward primer F4 and the reverse primer R5 (5′-AGCCCTGGATATAGTCAACAGG-3′).

    Techniques: Expressing, Knock-In, Mouse Assay, Western Blot, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, SYBR Green Assay

    Leptin up-regulated collagen expression in human LF cells by activating IL-6 expression RT-PCR and Western blot analyses showed that the mRNA ( B ) and protein ( A and C ) levels of collagen I and collagen III in hrleptin-treated cells were significantly elevated as compared with untreated cells (* P

    Journal: Bioscience Reports

    Article Title: Leptin-induced inflammation by activating IL-6 expression contributes to the fibrosis and hypertrophy of ligamentum flavum in lumbar spinal canal stenosis

    doi: 10.1042/BSR20171214

    Figure Lengend Snippet: Leptin up-regulated collagen expression in human LF cells by activating IL-6 expression RT-PCR and Western blot analyses showed that the mRNA ( B ) and protein ( A and C ) levels of collagen I and collagen III in hrleptin-treated cells were significantly elevated as compared with untreated cells (* P

    Article Snippet: Then, the gene expression levels of leptin, collagen I and collagen III, IL-6, and GAPDH were analyzed by fluorescent quantitative polymerase chain reaction (PCR) using a Thermal Cycler Dice Real-Time system (Takara, Dalian, China).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot