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  • 99
    Qiagen quantifast sybr green pcr kit
    Allele-specific forward primers allow detection of single and double BRAF mutations. A. Schematic diagrams of primer design. Forward primers were chosen to specifically amplify BRAF V600E1 (c.1799T > A), V600E2 (c.1799_1800delTGinsAA) and V600K (c.1798_1799delGTinsAA) mutations. Detection of BRAF V600K mutation can be performed separately or together with BRAF V600E mutation detection. WT = wild-type. B and C. Detection of low abundant BRAF V600E1 (B) and V600K mutations (C) in diluted DNA reference standards, which allelic frequencies were measured by droplet digital <t>PCR.</t> DNA amplification is monitored at each cycle of PCR using <t>SYBR</t> Green reagent included in PCR premix. D and E. Detection of BRAF V600E2 mutation (D) and complex tandem mutation caused by nucleotide changes in codons 600 and 601 (BRAF V600E1 and K601E mutations) (E). Synthesized oligonucleotides ( S1 Table ) were diluted and used as PCR templates.
    Quantifast Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 9575 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantifast sybr green pcr kit/product/Qiagen
    Average 99 stars, based on 9575 article reviews
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    quantifast sybr green pcr kit - by Bioz Stars, 2020-08
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    99
    Qiagen quantifast sybr green rt pcr kit qiagen
    Allele-specific forward primers allow detection of single and double BRAF mutations. A. Schematic diagrams of primer design. Forward primers were chosen to specifically amplify BRAF V600E1 (c.1799T > A), V600E2 (c.1799_1800delTGinsAA) and V600K (c.1798_1799delGTinsAA) mutations. Detection of BRAF V600K mutation can be performed separately or together with BRAF V600E mutation detection. WT = wild-type. B and C. Detection of low abundant BRAF V600E1 (B) and V600K mutations (C) in diluted DNA reference standards, which allelic frequencies were measured by droplet digital <t>PCR.</t> DNA amplification is monitored at each cycle of PCR using <t>SYBR</t> Green reagent included in PCR premix. D and E. Detection of BRAF V600E2 mutation (D) and complex tandem mutation caused by nucleotide changes in codons 600 and 601 (BRAF V600E1 and K601E mutations) (E). Synthesized oligonucleotides ( S1 Table ) were diluted and used as PCR templates.
    Quantifast Sybr Green Rt Pcr Kit Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantifast sybr green rt pcr kit qiagen/product/Qiagen
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    quantifast sybr green rt pcr kit qiagen - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    96
    Qiagen quantifast probe pcr kit
    Allele-specific forward primers allow detection of single and double BRAF mutations. A. Schematic diagrams of primer design. Forward primers were chosen to specifically amplify BRAF V600E1 (c.1799T > A), V600E2 (c.1799_1800delTGinsAA) and V600K (c.1798_1799delGTinsAA) mutations. Detection of BRAF V600K mutation can be performed separately or together with BRAF V600E mutation detection. WT = wild-type. B and C. Detection of low abundant BRAF V600E1 (B) and V600K mutations (C) in diluted DNA reference standards, which allelic frequencies were measured by droplet digital <t>PCR.</t> DNA amplification is monitored at each cycle of PCR using <t>SYBR</t> Green reagent included in PCR premix. D and E. Detection of BRAF V600E2 mutation (D) and complex tandem mutation caused by nucleotide changes in codons 600 and 601 (BRAF V600E1 and K601E mutations) (E). Synthesized oligonucleotides ( S1 Table ) were diluted and used as PCR templates.
    Quantifast Probe Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantifast probe pcr kit/product/Qiagen
    Average 96 stars, based on 405 article reviews
    Price from $9.99 to $1999.99
    quantifast probe pcr kit - by Bioz Stars, 2020-08
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      Buy from Supplier

    99
    Qiagen quantinova sybr green pcr kit
    Allele-specific forward primers allow detection of single and double BRAF mutations. A. Schematic diagrams of primer design. Forward primers were chosen to specifically amplify BRAF V600E1 (c.1799T > A), V600E2 (c.1799_1800delTGinsAA) and V600K (c.1798_1799delGTinsAA) mutations. Detection of BRAF V600K mutation can be performed separately or together with BRAF V600E mutation detection. WT = wild-type. B and C. Detection of low abundant BRAF V600E1 (B) and V600K mutations (C) in diluted DNA reference standards, which allelic frequencies were measured by droplet digital <t>PCR.</t> DNA amplification is monitored at each cycle of PCR using <t>SYBR</t> Green reagent included in PCR premix. D and E. Detection of BRAF V600E2 mutation (D) and complex tandem mutation caused by nucleotide changes in codons 600 and 601 (BRAF V600E1 and K601E mutations) (E). Synthesized oligonucleotides ( S1 Table ) were diluted and used as PCR templates.
    Quantinova Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1668 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantinova sybr green pcr kit/product/Qiagen
    Average 99 stars, based on 1668 article reviews
    Price from $9.99 to $1999.99
    quantinova sybr green pcr kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Allele-specific forward primers allow detection of single and double BRAF mutations. A. Schematic diagrams of primer design. Forward primers were chosen to specifically amplify BRAF V600E1 (c.1799T > A), V600E2 (c.1799_1800delTGinsAA) and V600K (c.1798_1799delGTinsAA) mutations. Detection of BRAF V600K mutation can be performed separately or together with BRAF V600E mutation detection. WT = wild-type. B and C. Detection of low abundant BRAF V600E1 (B) and V600K mutations (C) in diluted DNA reference standards, which allelic frequencies were measured by droplet digital PCR. DNA amplification is monitored at each cycle of PCR using SYBR Green reagent included in PCR premix. D and E. Detection of BRAF V600E2 mutation (D) and complex tandem mutation caused by nucleotide changes in codons 600 and 601 (BRAF V600E1 and K601E mutations) (E). Synthesized oligonucleotides ( S1 Table ) were diluted and used as PCR templates.

    Journal: PLoS ONE

    Article Title: Development of ultra-short PCR assay to reveal BRAF V600 mutation status in Thai colorectal cancer tissues

    doi: 10.1371/journal.pone.0198795

    Figure Lengend Snippet: Allele-specific forward primers allow detection of single and double BRAF mutations. A. Schematic diagrams of primer design. Forward primers were chosen to specifically amplify BRAF V600E1 (c.1799T > A), V600E2 (c.1799_1800delTGinsAA) and V600K (c.1798_1799delGTinsAA) mutations. Detection of BRAF V600K mutation can be performed separately or together with BRAF V600E mutation detection. WT = wild-type. B and C. Detection of low abundant BRAF V600E1 (B) and V600K mutations (C) in diluted DNA reference standards, which allelic frequencies were measured by droplet digital PCR. DNA amplification is monitored at each cycle of PCR using SYBR Green reagent included in PCR premix. D and E. Detection of BRAF V600E2 mutation (D) and complex tandem mutation caused by nucleotide changes in codons 600 and 601 (BRAF V600E1 and K601E mutations) (E). Synthesized oligonucleotides ( S1 Table ) were diluted and used as PCR templates.

    Article Snippet: Briefly, we used reaction mixture comprising 7.5μl of QuantiFAST SYBR Green PCR Kit including ROX dye (Qiagen, Germany), the total of 400 nM of BRAF mutant-specific primers, and 12–48 ng of genomic DNA for a 15-μl reaction.

    Techniques: Mutagenesis, Digital PCR, Amplification, Polymerase Chain Reaction, SYBR Green Assay, Synthesized

    Emergence of lateral roots in TaABCC13 :RNAi lines. (A) Relative transcript level of TaABCC13 in different RNAi lines of T 4 wheat seedlings. cDNA was prepared from 2 µg RNA (DNA free) and qRT-PCR analysis was performed using a SYBR Green-based assay. (B) Phenotypic analysis of the roots of C306 and transgenic RNAi lines. Ten seeds from TaABCC13 :RNAi and C306 were germinated on half-strength Hoagland media in a hydroponic system and observations were recorded at 10 d after germination. Each bar indicates the mean of three biological replicates (10 technical replicates).

    Journal: Journal of Experimental Botany

    Article Title: Silencing of ABCC13 transporter in wheat reveals its involvement in grain development, phytic acid accumulation and lateral root formation

    doi: 10.1093/jxb/erw224

    Figure Lengend Snippet: Emergence of lateral roots in TaABCC13 :RNAi lines. (A) Relative transcript level of TaABCC13 in different RNAi lines of T 4 wheat seedlings. cDNA was prepared from 2 µg RNA (DNA free) and qRT-PCR analysis was performed using a SYBR Green-based assay. (B) Phenotypic analysis of the roots of C306 and transgenic RNAi lines. Ten seeds from TaABCC13 :RNAi and C306 were germinated on half-strength Hoagland media in a hydroponic system and observations were recorded at 10 d after germination. Each bar indicates the mean of three biological replicates (10 technical replicates).

    Article Snippet: Quantitative real-time PCR analysis was performed by following SYBR Green (QuantiFastTM SYBR Green PCR kit, QIAGEN) chemistry using the ABI PRISM 7500 Fast Realtime Platform (Applied Biosystems).

    Techniques: Quantitative RT-PCR, SYBR Green Assay, Transgenic Assay

    Silencing in seeds, phytic acid estimation, and seed quality of TaABCC13 :RNAi lines. (A) Relative transcript levels of TaABCC13 in different RNAi lines of wheat. Total RNA was isolated at 14 d after anthesis of the primary tiller of non-segregating T 4 RNAi lines, cDNA was prepared from 2 µg of RNA (DNA free) and qRT-PCR analysis was performed using a SYBR Green-based assay. (B) Estimation of total phytic acid in mature wheat grains of transgenic lines. Seeds were collected from the primary tiller of each line. (C) Average seed weight of the TaABCC13: RNAi lines was determined by weighing 50 random seeds. (D) The total protein content of seeds from the TaABCC13: RNAi lines and non-transgenic parent was determined by using the Bradford method. Each bar indicates the mean of three biological replicates (three technical replicates). ** indicates significant differences at P

    Journal: Journal of Experimental Botany

    Article Title: Silencing of ABCC13 transporter in wheat reveals its involvement in grain development, phytic acid accumulation and lateral root formation

    doi: 10.1093/jxb/erw224

    Figure Lengend Snippet: Silencing in seeds, phytic acid estimation, and seed quality of TaABCC13 :RNAi lines. (A) Relative transcript levels of TaABCC13 in different RNAi lines of wheat. Total RNA was isolated at 14 d after anthesis of the primary tiller of non-segregating T 4 RNAi lines, cDNA was prepared from 2 µg of RNA (DNA free) and qRT-PCR analysis was performed using a SYBR Green-based assay. (B) Estimation of total phytic acid in mature wheat grains of transgenic lines. Seeds were collected from the primary tiller of each line. (C) Average seed weight of the TaABCC13: RNAi lines was determined by weighing 50 random seeds. (D) The total protein content of seeds from the TaABCC13: RNAi lines and non-transgenic parent was determined by using the Bradford method. Each bar indicates the mean of three biological replicates (three technical replicates). ** indicates significant differences at P

    Article Snippet: Quantitative real-time PCR analysis was performed by following SYBR Green (QuantiFastTM SYBR Green PCR kit, QIAGEN) chemistry using the ABI PRISM 7500 Fast Realtime Platform (Applied Biosystems).

    Techniques: Isolation, Quantitative RT-PCR, SYBR Green Assay, Transgenic Assay