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  • 99
    Thermo Fisher rna
    Comparison of <t>RNA</t> extraction methods . RNA yields under three different extraction methods: TRIzol ® , Maxwell ® and Maxwell ® plus the addition of BME. Three amounts of peanut hairy root were evaluated: 10, 20 and 40 mg DW. Lyophilized tissue of 9-day old root culture was extracted under each method. RNA was quantified by Quant-iT™ <t>RiboGreen</t> ® RNA kit. Data shown represents a single replicate per method at 3 distinct amounts of starting material.
    Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 177519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it picogreen dsdna assay kit thermo fisher scientific
    Comparison of <t>RNA</t> extraction methods . RNA yields under three different extraction methods: TRIzol ® , Maxwell ® and Maxwell ® plus the addition of BME. Three amounts of peanut hairy root were evaluated: 10, 20 and 40 mg DW. Lyophilized tissue of 9-day old root culture was extracted under each method. RNA was quantified by Quant-iT™ <t>RiboGreen</t> ® RNA kit. Data shown represents a single replicate per method at 3 distinct amounts of starting material.
    Quant It Picogreen Dsdna Assay Kit Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it rna
    <t>RNA</t> content was determined using the <t>Qubit</t> quantitation system. The amount of RNA is represented in micrograms of RNA per gram of dry alginate ± SEM. RNA assay sensitivity is 5–100 ng per sample. * p
    Quant It Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it rna assay
    Cellular <t>RNA</t> and miR-146a mimics stimulate cfB production in vivo via TLRs signaling RNA (50 μg/mouse) or miR-146a (20 μg/mouse) complexed with <t>lipofectamine</t> was injected i.p. into WT, MyD88 −/− or TLR7 −/− mice with lipofectamine or mutant as the respective control. Twenty hours later, the peritoneal lavage was harvested and cfB protein expression was analyzed by Western blot. The cfB expression levels were quantitated by a NIH ImageJ software and expressed as fold change over Lipofectamine control (A) or no injection group (None, B) in WT mice. A. cfB expression in the peritoneal lavage after RNA injection. n=4 per group, * P
    Quant It Rna Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quant it rna assay/product/Thermo Fisher
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    Thermo Fisher rna quantitation kit
    Cellular <t>RNA</t> and miR-146a mimics stimulate cfB production in vivo via TLRs signaling RNA (50 μg/mouse) or miR-146a (20 μg/mouse) complexed with <t>lipofectamine</t> was injected i.p. into WT, MyD88 −/− or TLR7 −/− mice with lipofectamine or mutant as the respective control. Twenty hours later, the peritoneal lavage was harvested and cfB protein expression was analyzed by Western blot. The cfB expression levels were quantitated by a NIH ImageJ software and expressed as fold change over Lipofectamine control (A) or no injection group (None, B) in WT mice. A. cfB expression in the peritoneal lavage after RNA injection. n=4 per group, * P
    Rna Quantitation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Comparison of RNA extraction methods . RNA yields under three different extraction methods: TRIzol ® , Maxwell ® and Maxwell ® plus the addition of BME. Three amounts of peanut hairy root were evaluated: 10, 20 and 40 mg DW. Lyophilized tissue of 9-day old root culture was extracted under each method. RNA was quantified by Quant-iT™ RiboGreen ® RNA kit. Data shown represents a single replicate per method at 3 distinct amounts of starting material.

    Journal: BMC Research Notes

    Article Title: Selection of reference genes for qPCR in hairy root cultures of peanut

    doi: 10.1186/1756-0500-4-392

    Figure Lengend Snippet: Comparison of RNA extraction methods . RNA yields under three different extraction methods: TRIzol ® , Maxwell ® and Maxwell ® plus the addition of BME. Three amounts of peanut hairy root were evaluated: 10, 20 and 40 mg DW. Lyophilized tissue of 9-day old root culture was extracted under each method. RNA was quantified by Quant-iT™ RiboGreen ® RNA kit. Data shown represents a single replicate per method at 3 distinct amounts of starting material.

    Article Snippet: RNA concentration was determined using Quant-iT™ RiboGreen® RNA kit (Invitrogen) using the following modified method for a 96-well microplate format.

    Techniques: RNA Extraction

    Average recovery of total RNA from kits using manufacturer-provided protocols. Total RNA yield was assessed by RiboGreen Quant-it assay (ng) from 200 μL of plasma. Isolations were performed in triplicate and the average displayed. There is variability

    Journal: RNA

    Article Title: Identification of extracellular miRNA in human cerebrospinal fluid by next-generation sequencing

    doi: 10.1261/rna.036863.112

    Figure Lengend Snippet: Average recovery of total RNA from kits using manufacturer-provided protocols. Total RNA yield was assessed by RiboGreen Quant-it assay (ng) from 200 μL of plasma. Isolations were performed in triplicate and the average displayed. There is variability

    Article Snippet: Quantification of the total RNA yield was determined by Quant-iT RiboGreen RNA reagent (Invitrogen).

    Techniques:

    Quantitative Northern blot analysis of TLC1 molecules in total RNA. ( A ) The leftmost and rightmost sets of lanes, respectively, show twofold serial dilutions of in vitro transcribed TLC1 1167 RNA (beginning with 6.25 × 10 8 molecules) and TLC1 1261A55 RNA (beginning with 3.12 × 10 8 molecules). The center lanes show 10 μg, 7.5 μg, and 5 μg total RNA isolated from wild-type haploid cells (WT N), wild-type diploid cells (WT 2N), and TLC1/tlc1Δ heterozygous diploid cells (Het 2N). RNAs were hybridized with TLC1 and U1 snRNA probes. ( B ) A standard curve for TLC1 quantification was made by plotting the counts per peak area against the known amount of each standard in the TLC1 1167 RNA dilution series. Linear regression analysis of the seven-point curve yielded an r 2 value of 0.997, indicating that the Northern assay is linear from 9.76 × 10 6 to 6.25 × 10 8 TLC1 molecules; only the five lower points are shown in the graph because the interpolated TLC1 copy numbers in total RNA fall within this lower range. The TLC1 signal in each 10 μg total RNA sample is plotted on the line. ( C ) The number of TLC1 molecules in each total RNA sample was interpolated from either the TLC1 1167 standard curve or the TLC1 1261A55 standard curve (r 2 = 0.998). TLC1 molecules per nanogram total RNA were calculated by dividing the interpolated number by the amount of RNA loaded, i.e., either 10 μg, 7.5 μg, or 5 μg. To express these numbers relative to the A 260 quantification of the RNA standards, we then multiplied the RiboGreen-based values by the concentration ratio of A 260 /RiboGreen (see Materials and Methods). TLC1 molecules per cell were calculated by multiplying TLC1 molecules per nanogram RNA by 0.0012 ng RNA per haploid cell or 0.0018 ng RNA per diploid cell. Each number shown is the average of the 10 μg, 7.5 μg, and 5 μg samples, with the standard deviations shown in parentheses.

    Journal: RNA

    Article Title: Low abundance of telomerase in yeast: Implications for telomerase haploinsufficiency

    doi: 10.1261/rna.134706

    Figure Lengend Snippet: Quantitative Northern blot analysis of TLC1 molecules in total RNA. ( A ) The leftmost and rightmost sets of lanes, respectively, show twofold serial dilutions of in vitro transcribed TLC1 1167 RNA (beginning with 6.25 × 10 8 molecules) and TLC1 1261A55 RNA (beginning with 3.12 × 10 8 molecules). The center lanes show 10 μg, 7.5 μg, and 5 μg total RNA isolated from wild-type haploid cells (WT N), wild-type diploid cells (WT 2N), and TLC1/tlc1Δ heterozygous diploid cells (Het 2N). RNAs were hybridized with TLC1 and U1 snRNA probes. ( B ) A standard curve for TLC1 quantification was made by plotting the counts per peak area against the known amount of each standard in the TLC1 1167 RNA dilution series. Linear regression analysis of the seven-point curve yielded an r 2 value of 0.997, indicating that the Northern assay is linear from 9.76 × 10 6 to 6.25 × 10 8 TLC1 molecules; only the five lower points are shown in the graph because the interpolated TLC1 copy numbers in total RNA fall within this lower range. The TLC1 signal in each 10 μg total RNA sample is plotted on the line. ( C ) The number of TLC1 molecules in each total RNA sample was interpolated from either the TLC1 1167 standard curve or the TLC1 1261A55 standard curve (r 2 = 0.998). TLC1 molecules per nanogram total RNA were calculated by dividing the interpolated number by the amount of RNA loaded, i.e., either 10 μg, 7.5 μg, or 5 μg. To express these numbers relative to the A 260 quantification of the RNA standards, we then multiplied the RiboGreen-based values by the concentration ratio of A 260 /RiboGreen (see Materials and Methods). TLC1 molecules per cell were calculated by multiplying TLC1 molecules per nanogram RNA by 0.0012 ng RNA per haploid cell or 0.0018 ng RNA per diploid cell. Each number shown is the average of the 10 μg, 7.5 μg, and 5 μg samples, with the standard deviations shown in parentheses.

    Article Snippet: The in vitro transcribed standard RNAs were quantified with the RiboGreen RNA Quantitation Kit (Molecular Probes) essentially according to the pack insert, with the following modifications to monitor fluorescence using smaller assay volumes in a LightCycler 2.0 (Roche).

    Techniques: Northern Blot, In Vitro, Isolation, Concentration Assay

    RNA content was determined using the Qubit quantitation system. The amount of RNA is represented in micrograms of RNA per gram of dry alginate ± SEM. RNA assay sensitivity is 5–100 ng per sample. * p

    Journal: Journal of biomaterials applications

    Article Title: Optimization of alginate purification using polyvinylidene difluoride membrane filtration: Effects on immunogenicity and biocompatibility of three-dimensional alginate scaffolds

    doi: 10.1177/0885328216645952

    Figure Lengend Snippet: RNA content was determined using the Qubit quantitation system. The amount of RNA is represented in micrograms of RNA per gram of dry alginate ± SEM. RNA assay sensitivity is 5–100 ng per sample. * p

    Article Snippet: DNA and RNA concentrations in alginate solutions were determined using the Qubit Quant-iT quantitation system using the Qubit Fluorometer ( ) with the Quant-iT dsDNA HS ( ) and RNA HS ( ) assays (Invitrogen, Carlsbad, CA).

    Techniques: Quantitation Assay

    Cellular RNA and miR-146a mimics stimulate cfB production in vivo via TLRs signaling RNA (50 μg/mouse) or miR-146a (20 μg/mouse) complexed with lipofectamine was injected i.p. into WT, MyD88 −/− or TLR7 −/− mice with lipofectamine or mutant as the respective control. Twenty hours later, the peritoneal lavage was harvested and cfB protein expression was analyzed by Western blot. The cfB expression levels were quantitated by a NIH ImageJ software and expressed as fold change over Lipofectamine control (A) or no injection group (None, B) in WT mice. A. cfB expression in the peritoneal lavage after RNA injection. n=4 per group, * P

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Splenic RNA and microRNA mimics promote complement factor B production and the alternative pathway activation via innate immune signaling

    doi: 10.4049/jimmunol.1502106

    Figure Lengend Snippet: Cellular RNA and miR-146a mimics stimulate cfB production in vivo via TLRs signaling RNA (50 μg/mouse) or miR-146a (20 μg/mouse) complexed with lipofectamine was injected i.p. into WT, MyD88 −/− or TLR7 −/− mice with lipofectamine or mutant as the respective control. Twenty hours later, the peritoneal lavage was harvested and cfB protein expression was analyzed by Western blot. The cfB expression levels were quantitated by a NIH ImageJ software and expressed as fold change over Lipofectamine control (A) or no injection group (None, B) in WT mice. A. cfB expression in the peritoneal lavage after RNA injection. n=4 per group, * P

    Article Snippet: Bovine pancreas RNase A, lipofectamine 3000, TRIzol LS, SYTO RNASelect Green fluorescent cell stain, and Quant-iT™ RNA assay kit were from Invitrogen Life Technology (Carlsbad, CA).

    Techniques: In Vivo, Injection, Mouse Assay, Mutagenesis, Expressing, Western Blot, Software

    Splenic RNA-induced cfB production was mediated via MyD88 signaling in macrophages A. Complement gene expression in macrophages treated with splenic RNA. Mouse macrophages were treated with lipofectamine alone or splenic RNA (10 μg/ml) complexed with lipofectamine. Six hours later, C3, C4, C5, cfB mRNA was analyzed by qRT-PCR. *** P

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Splenic RNA and microRNA mimics promote complement factor B production and the alternative pathway activation via innate immune signaling

    doi: 10.4049/jimmunol.1502106

    Figure Lengend Snippet: Splenic RNA-induced cfB production was mediated via MyD88 signaling in macrophages A. Complement gene expression in macrophages treated with splenic RNA. Mouse macrophages were treated with lipofectamine alone or splenic RNA (10 μg/ml) complexed with lipofectamine. Six hours later, C3, C4, C5, cfB mRNA was analyzed by qRT-PCR. *** P

    Article Snippet: Bovine pancreas RNase A, lipofectamine 3000, TRIzol LS, SYTO RNASelect Green fluorescent cell stain, and Quant-iT™ RNA assay kit were from Invitrogen Life Technology (Carlsbad, CA).

    Techniques: Expressing, Quantitative RT-PCR