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  • 90
    Thermo Fisher quant it hs reagents
    Quant It Hs Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it ribogreen rna reagent
    Average recovery of total <t>RNA</t> from kits using manufacturer-provided protocols. Total RNA yield was assessed by <t>RiboGreen</t> Quant-it assay (ng) from 200 μL of plasma. Isolations were performed in triplicate and the average displayed. There is variability
    Quant It Ribogreen Rna Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it rna assay kit
    Cellular <t>RNA</t> and miR-146a mimics stimulate cfB production in vivo via TLRs signaling RNA (50 μg/mouse) or miR-146a (20 μg/mouse) complexed with <t>lipofectamine</t> was injected i.p. into WT, MyD88 −/− or TLR7 −/− mice with lipofectamine or mutant as the respective control. Twenty hours later, the peritoneal lavage was harvested and cfB protein expression was analyzed by Western blot. The cfB expression levels were quantitated by a NIH ImageJ software and expressed as fold change over Lipofectamine control (A) or no injection group (None, B) in WT mice. A. cfB expression in the peritoneal lavage after RNA injection. n=4 per group, * P
    Quant It Rna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellular <t>RNA</t> and miR-146a mimics stimulate cfB production in vivo via TLRs signaling RNA (50 μg/mouse) or miR-146a (20 μg/mouse) complexed with <t>lipofectamine</t> was injected i.p. into WT, MyD88 −/− or TLR7 −/− mice with lipofectamine or mutant as the respective control. Twenty hours later, the peritoneal lavage was harvested and cfB protein expression was analyzed by Western blot. The cfB expression levels were quantitated by a NIH ImageJ software and expressed as fold change over Lipofectamine control (A) or no injection group (None, B) in WT mice. A. cfB expression in the peritoneal lavage after RNA injection. n=4 per group, * P
    Quant It Ribogreen Rna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Weak organic acids reduce total <t>RNA</t> and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by <t>RiboGreen</t> assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P
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    Thermo Fisher quanti ittm ribogreen rna assay kit
    Weak organic acids reduce total <t>RNA</t> and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by <t>RiboGreen</t> assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P
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    Thermo Fisher qubit rna hs assay kit
    Weak organic acids reduce total <t>RNA</t> and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by <t>RiboGreen</t> assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P
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    Weak organic acids reduce total <t>RNA</t> and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by <t>RiboGreen</t> assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P
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    Thermo Fisher quant ittm rna broad range kit
    Weak organic acids reduce total <t>RNA</t> and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by <t>RiboGreen</t> assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P
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    Weak organic acids reduce total <t>RNA</t> and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by <t>RiboGreen</t> assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P
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    Thermo Fisher quant it ribogreen rna reagent assay kit
    Weak organic acids reduce total <t>RNA</t> and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by <t>RiboGreen</t> assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P
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    Weak organic acids reduce total <t>RNA</t> and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by <t>RiboGreen</t> assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P
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    Weak organic acids reduce total <t>RNA</t> and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by <t>RiboGreen</t> assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P
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    Thermo Fisher quant it picogreen dsdna assay kit
    (a) Enhancement of hMSC proliferation using US. MSCs were grown on glass coverslips for 4 days in M2 medium with and without US (14 kPa; 3 min; 1, 2, or 4 times/day). Cells were fixed and stained for Ki67 (mitosis marker) and Hoechst (nuclear marker). Five pictures were randomly taken on three coverslips per condition ( n = 15), and Ki67 and Hoechst positive cells were counted using ImageJ ™ . Data were expressed as percent Ki67 positive. (b) hMSC proliferation is increased with US application. hMSC-seeded scaffolds were cultured in CDM for 14 days and in M3 media for another 7 days in the US-assisted bioreactor according to the culture conditions outlined in Table 2 . <t>dsDNA</t> was assayed by <t>Picogreen</t> assay. Data were presented as average ± standard deviation ( n = 5–8 constructs). Statistically significant data were accounted with respect to respective control and shown as * p
    Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10071 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ribogreen quantification
    (a) Enhancement of hMSC proliferation using US. MSCs were grown on glass coverslips for 4 days in M2 medium with and without US (14 kPa; 3 min; 1, 2, or 4 times/day). Cells were fixed and stained for Ki67 (mitosis marker) and Hoechst (nuclear marker). Five pictures were randomly taken on three coverslips per condition ( n = 15), and Ki67 and Hoechst positive cells were counted using ImageJ ™ . Data were expressed as percent Ki67 positive. (b) hMSC proliferation is increased with US application. hMSC-seeded scaffolds were cultured in CDM for 14 days and in M3 media for another 7 days in the US-assisted bioreactor according to the culture conditions outlined in Table 2 . <t>dsDNA</t> was assayed by <t>Picogreen</t> assay. Data were presented as average ± standard deviation ( n = 5–8 constructs). Statistically significant data were accounted with respect to respective control and shown as * p
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    Thermo Fisher quant it dsdna hs kit
    RSV triggers the release of NETs coated with NE and MPO. ( A – D ) Neutrophils (1 × 10 5 /300 μL) were stimulated with active RSV (10 4 PFU/mL) or UV-inactivated RSV (10 4 PFU/mL) for 180 min at 37 °C with 5% CO 2 in 8-chamber culture slides. Afterwards, cells were fixed with 4% PFA and stained with (A) Hoechst 33342 (1:2000), anti-RSV fusion protein (1:1000) followed by anti-mouse PE antibody (1:500); (B) Hoechst 33342 (1:2000), anti-elastase (NE; 1:1000) followed by anti-rabbit Cy3 antibody (1:500); (C) Hoechst 33342 (1:2000), anti-myeloperoxidase PE (MPO, 1:1000) antibody; (D) Hoechst 33342 (1:2000). Overlay of the fluorescence images are shown in the penultimate panels. Arrowheads indicate the presence of extracellular <t>DNA</t> lattices co-localized with RSV F protein, NE and MPO, respectively. NETs were magnified four times and are numbered (1, 2, 3 and 4) on the right side. Images are representative of 3 independent experiments. Images were taken in a Zeiss LSM 5 Exciter microscope. Scale bars = 5 μm. (E) Neutrophils (1 × 10 6 /mL) were stimulated with active RSV (10 4 PFU/mL) or UV-inactivated RSV (10 4 PFU/mL) for 180 min at 37 °C with 5% CO 2 . Afterwards, NETs were quantified in culture supernatants using Quant-iT <t>dsDNA</t> HS kit (Invitrogen). Data are representative of 2 independent experiments performed in triplicates and represent mean ± SEM. Data were analyzed with Mann Whitney test. *p
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    HuR is required for short-term proliferation of PDA cells Relative proliferation of DOX-inducible MIA PaCa-2 cell lines treated with 0 or 2 μg/ml DOX for the indicated time points, as determined by measurement of <t>dsDNA</t> content by <t>PicoGreen</t> staining. Each data point represents the mean of 5 independent experiments ± standard error of the mean (SEM). * = p
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    Long <t>ssDNA</t> target sequences (black) are digested by S1 nuclease, leaving intact only those regions bound to the PNA probe (red). CPs (blue) added directly to the resulting solutions can only associate with the remaining <t>PNA-FL/DNA</t> duplex. Any PNA/DNA mismatches will result in complete DNA digestion; therefore, energy transfer from the CP occurs only for the perfect PNA-FL/DNA complement.
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    Thermo Fisher quant it assay kit
    Long <t>ssDNA</t> target sequences (black) are digested by S1 nuclease, leaving intact only those regions bound to the PNA probe (red). CPs (blue) added directly to the resulting solutions can only associate with the remaining <t>PNA-FL/DNA</t> duplex. Any PNA/DNA mismatches will result in complete DNA digestion; therefore, energy transfer from the CP occurs only for the perfect PNA-FL/DNA complement.
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    Long <t>ssDNA</t> target sequences (black) are digested by S1 nuclease, leaving intact only those regions bound to the PNA probe (red). CPs (blue) added directly to the resulting solutions can only associate with the remaining <t>PNA-FL/DNA</t> duplex. Any PNA/DNA mismatches will result in complete DNA digestion; therefore, energy transfer from the CP occurs only for the perfect PNA-FL/DNA complement.
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    Long <t>ssDNA</t> target sequences (black) are digested by S1 nuclease, leaving intact only those regions bound to the PNA probe (red). CPs (blue) added directly to the resulting solutions can only associate with the remaining <t>PNA-FL/DNA</t> duplex. Any PNA/DNA mismatches will result in complete DNA digestion; therefore, energy transfer from the CP occurs only for the perfect PNA-FL/DNA complement.
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    Bland-Altman Plot of Difference <t>(Qubit-Nanodrop)</t> (ng/μL) vs. average <t>DNA</t> yield (mean of Qubit and Nanodrop).
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    Bland-Altman Plot of Difference <t>(Qubit-Nanodrop)</t> (ng/μL) vs. average <t>DNA</t> yield (mean of Qubit and Nanodrop).
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    Image Search Results


    Average recovery of total RNA from kits using manufacturer-provided protocols. Total RNA yield was assessed by RiboGreen Quant-it assay (ng) from 200 μL of plasma. Isolations were performed in triplicate and the average displayed. There is variability

    Journal: RNA

    Article Title: Identification of extracellular miRNA in human cerebrospinal fluid by next-generation sequencing

    doi: 10.1261/rna.036863.112

    Figure Lengend Snippet: Average recovery of total RNA from kits using manufacturer-provided protocols. Total RNA yield was assessed by RiboGreen Quant-it assay (ng) from 200 μL of plasma. Isolations were performed in triplicate and the average displayed. There is variability

    Article Snippet: Quantification of the total RNA yield was determined by Quant-iT RiboGreen RNA reagent (Invitrogen).

    Techniques:

    Cellular RNA and miR-146a mimics stimulate cfB production in vivo via TLRs signaling RNA (50 μg/mouse) or miR-146a (20 μg/mouse) complexed with lipofectamine was injected i.p. into WT, MyD88 −/− or TLR7 −/− mice with lipofectamine or mutant as the respective control. Twenty hours later, the peritoneal lavage was harvested and cfB protein expression was analyzed by Western blot. The cfB expression levels were quantitated by a NIH ImageJ software and expressed as fold change over Lipofectamine control (A) or no injection group (None, B) in WT mice. A. cfB expression in the peritoneal lavage after RNA injection. n=4 per group, * P

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Splenic RNA and microRNA mimics promote complement factor B production and the alternative pathway activation via innate immune signaling

    doi: 10.4049/jimmunol.1502106

    Figure Lengend Snippet: Cellular RNA and miR-146a mimics stimulate cfB production in vivo via TLRs signaling RNA (50 μg/mouse) or miR-146a (20 μg/mouse) complexed with lipofectamine was injected i.p. into WT, MyD88 −/− or TLR7 −/− mice with lipofectamine or mutant as the respective control. Twenty hours later, the peritoneal lavage was harvested and cfB protein expression was analyzed by Western blot. The cfB expression levels were quantitated by a NIH ImageJ software and expressed as fold change over Lipofectamine control (A) or no injection group (None, B) in WT mice. A. cfB expression in the peritoneal lavage after RNA injection. n=4 per group, * P

    Article Snippet: Bovine pancreas RNase A, lipofectamine 3000, TRIzol LS, SYTO RNASelect Green fluorescent cell stain, and Quant-iT™ RNA assay kit were from Invitrogen Life Technology (Carlsbad, CA).

    Techniques: In Vivo, Injection, Mouse Assay, Mutagenesis, Expressing, Western Blot, Software

    Splenic RNA-induced cfB production was mediated via MyD88 signaling in macrophages A. Complement gene expression in macrophages treated with splenic RNA. Mouse macrophages were treated with lipofectamine alone or splenic RNA (10 μg/ml) complexed with lipofectamine. Six hours later, C3, C4, C5, cfB mRNA was analyzed by qRT-PCR. *** P

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Splenic RNA and microRNA mimics promote complement factor B production and the alternative pathway activation via innate immune signaling

    doi: 10.4049/jimmunol.1502106

    Figure Lengend Snippet: Splenic RNA-induced cfB production was mediated via MyD88 signaling in macrophages A. Complement gene expression in macrophages treated with splenic RNA. Mouse macrophages were treated with lipofectamine alone or splenic RNA (10 μg/ml) complexed with lipofectamine. Six hours later, C3, C4, C5, cfB mRNA was analyzed by qRT-PCR. *** P

    Article Snippet: Bovine pancreas RNase A, lipofectamine 3000, TRIzol LS, SYTO RNASelect Green fluorescent cell stain, and Quant-iT™ RNA assay kit were from Invitrogen Life Technology (Carlsbad, CA).

    Techniques: Expressing, Quantitative RT-PCR

    Weak organic acids reduce total RNA and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by RiboGreen assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P

    Journal: G3: Genes|Genomes|Genetics

    Article Title: The Transcriptional Stress Response of Candida albicans to Weak Organic Acids

    doi: 10.1534/g3.114.015941

    Figure Lengend Snippet: Weak organic acids reduce total RNA and ribosomal RNA in Candida albicans : Total RNA content and relative rRNA abundance in cell pellets used for RNA deep-sequencing experiment. (A) Total RNA extraction yield quantified by RiboGreen assay. (B) rRNA vs. total RNA ratio estimated by areas under the peaks found on Bioanalyzer electropherograms. n = 4; * P

    Article Snippet: Total RNA yield was quantified with the Quant-iT RiboGreen RNA Assay Kit (Invitrogen) and RNA quality assessed on the 2100 Bioanalyzer (Agilent).

    Techniques: Sequencing, RNA Extraction

    (a) Enhancement of hMSC proliferation using US. MSCs were grown on glass coverslips for 4 days in M2 medium with and without US (14 kPa; 3 min; 1, 2, or 4 times/day). Cells were fixed and stained for Ki67 (mitosis marker) and Hoechst (nuclear marker). Five pictures were randomly taken on three coverslips per condition ( n = 15), and Ki67 and Hoechst positive cells were counted using ImageJ ™ . Data were expressed as percent Ki67 positive. (b) hMSC proliferation is increased with US application. hMSC-seeded scaffolds were cultured in CDM for 14 days and in M3 media for another 7 days in the US-assisted bioreactor according to the culture conditions outlined in Table 2 . dsDNA was assayed by Picogreen assay. Data were presented as average ± standard deviation ( n = 5–8 constructs). Statistically significant data were accounted with respect to respective control and shown as * p

    Journal: Journal of Tissue Engineering

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis

    doi: 10.1177/2041731414566529

    Figure Lengend Snippet: (a) Enhancement of hMSC proliferation using US. MSCs were grown on glass coverslips for 4 days in M2 medium with and without US (14 kPa; 3 min; 1, 2, or 4 times/day). Cells were fixed and stained for Ki67 (mitosis marker) and Hoechst (nuclear marker). Five pictures were randomly taken on three coverslips per condition ( n = 15), and Ki67 and Hoechst positive cells were counted using ImageJ ™ . Data were expressed as percent Ki67 positive. (b) hMSC proliferation is increased with US application. hMSC-seeded scaffolds were cultured in CDM for 14 days and in M3 media for another 7 days in the US-assisted bioreactor according to the culture conditions outlined in Table 2 . dsDNA was assayed by Picogreen assay. Data were presented as average ± standard deviation ( n = 5–8 constructs). Statistically significant data were accounted with respect to respective control and shown as * p

    Article Snippet: Double-stranded DNA (dsDNA) was measured using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) according to manufacturer’s instructions.

    Techniques: Staining, Marker, Cell Culture, Picogreen Assay, Standard Deviation, Construct

    RSV triggers the release of NETs coated with NE and MPO. ( A – D ) Neutrophils (1 × 10 5 /300 μL) were stimulated with active RSV (10 4 PFU/mL) or UV-inactivated RSV (10 4 PFU/mL) for 180 min at 37 °C with 5% CO 2 in 8-chamber culture slides. Afterwards, cells were fixed with 4% PFA and stained with (A) Hoechst 33342 (1:2000), anti-RSV fusion protein (1:1000) followed by anti-mouse PE antibody (1:500); (B) Hoechst 33342 (1:2000), anti-elastase (NE; 1:1000) followed by anti-rabbit Cy3 antibody (1:500); (C) Hoechst 33342 (1:2000), anti-myeloperoxidase PE (MPO, 1:1000) antibody; (D) Hoechst 33342 (1:2000). Overlay of the fluorescence images are shown in the penultimate panels. Arrowheads indicate the presence of extracellular DNA lattices co-localized with RSV F protein, NE and MPO, respectively. NETs were magnified four times and are numbered (1, 2, 3 and 4) on the right side. Images are representative of 3 independent experiments. Images were taken in a Zeiss LSM 5 Exciter microscope. Scale bars = 5 μm. (E) Neutrophils (1 × 10 6 /mL) were stimulated with active RSV (10 4 PFU/mL) or UV-inactivated RSV (10 4 PFU/mL) for 180 min at 37 °C with 5% CO 2 . Afterwards, NETs were quantified in culture supernatants using Quant-iT dsDNA HS kit (Invitrogen). Data are representative of 2 independent experiments performed in triplicates and represent mean ± SEM. Data were analyzed with Mann Whitney test. *p

    Journal: Scientific Reports

    Article Title: Respiratory Syncytial Virus induces the classical ROS-dependent NETosis through PAD-4 and necroptosis pathways activation

    doi: 10.1038/s41598-018-32576-y

    Figure Lengend Snippet: RSV triggers the release of NETs coated with NE and MPO. ( A – D ) Neutrophils (1 × 10 5 /300 μL) were stimulated with active RSV (10 4 PFU/mL) or UV-inactivated RSV (10 4 PFU/mL) for 180 min at 37 °C with 5% CO 2 in 8-chamber culture slides. Afterwards, cells were fixed with 4% PFA and stained with (A) Hoechst 33342 (1:2000), anti-RSV fusion protein (1:1000) followed by anti-mouse PE antibody (1:500); (B) Hoechst 33342 (1:2000), anti-elastase (NE; 1:1000) followed by anti-rabbit Cy3 antibody (1:500); (C) Hoechst 33342 (1:2000), anti-myeloperoxidase PE (MPO, 1:1000) antibody; (D) Hoechst 33342 (1:2000). Overlay of the fluorescence images are shown in the penultimate panels. Arrowheads indicate the presence of extracellular DNA lattices co-localized with RSV F protein, NE and MPO, respectively. NETs were magnified four times and are numbered (1, 2, 3 and 4) on the right side. Images are representative of 3 independent experiments. Images were taken in a Zeiss LSM 5 Exciter microscope. Scale bars = 5 μm. (E) Neutrophils (1 × 10 6 /mL) were stimulated with active RSV (10 4 PFU/mL) or UV-inactivated RSV (10 4 PFU/mL) for 180 min at 37 °C with 5% CO 2 . Afterwards, NETs were quantified in culture supernatants using Quant-iT dsDNA HS kit (Invitrogen). Data are representative of 2 independent experiments performed in triplicates and represent mean ± SEM. Data were analyzed with Mann Whitney test. *p

    Article Snippet: After the stimulation period, culture supernatant was collected and extracellular DNA was measured using the dsDNA Picogreen kit or Quant-iT dsDNA HS kit (both from Invitrogen), following manufacturer’s instructions and obtaining similar results.

    Techniques: Staining, Fluorescence, Microscopy, MANN-WHITNEY

    HuR is required for short-term proliferation of PDA cells Relative proliferation of DOX-inducible MIA PaCa-2 cell lines treated with 0 or 2 μg/ml DOX for the indicated time points, as determined by measurement of dsDNA content by PicoGreen staining. Each data point represents the mean of 5 independent experiments ± standard error of the mean (SEM). * = p

    Journal: Oncotarget

    Article Title: Targeting the mRNA-binding protein HuR impairs malignant characteristics of pancreatic ductal adenocarcinoma cells

    doi:

    Figure Lengend Snippet: HuR is required for short-term proliferation of PDA cells Relative proliferation of DOX-inducible MIA PaCa-2 cell lines treated with 0 or 2 μg/ml DOX for the indicated time points, as determined by measurement of dsDNA content by PicoGreen staining. Each data point represents the mean of 5 independent experiments ± standard error of the mean (SEM). * = p

    Article Snippet: Double-stranded DNA (dsDNA) was stained by Quant-iT PicoGreen dsDNA reagent (Life Technologies, cat. #P7581), and fluorescence intensity was measured by a microplate reader (Tecan, part #F129015) using excitation wavelength of 485 nm and emission wavelength of 535 nm.

    Techniques: Staining

    Long ssDNA target sequences (black) are digested by S1 nuclease, leaving intact only those regions bound to the PNA probe (red). CPs (blue) added directly to the resulting solutions can only associate with the remaining PNA-FL/DNA duplex. Any PNA/DNA mismatches will result in complete DNA digestion; therefore, energy transfer from the CP occurs only for the perfect PNA-FL/DNA complement.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: SNP detection using peptide nucleic acid probes and conjugated polymers: Applications in neurodegenerative disease identification

    doi: 10.1073/pnas.0407578101

    Figure Lengend Snippet: Long ssDNA target sequences (black) are digested by S1 nuclease, leaving intact only those regions bound to the PNA probe (red). CPs (blue) added directly to the resulting solutions can only associate with the remaining PNA-FL/DNA duplex. Any PNA/DNA mismatches will result in complete DNA digestion; therefore, energy transfer from the CP occurs only for the perfect PNA-FL/DNA complement.

    Article Snippet: Concentrations of the larger DNA sequences obtained by PCR and gel purification were measured by fluorescence using an OliGreen single stranded DNA (ssDNA) Quantitation kit (Molecular Probes).

    Techniques:

    Cationic CPs (blue) have the ability to associate nonspecifically along any region of a targeted ssDNA sequence (black). ( A ) Association of a short DNA target with the CP. ( B ) The probability of CP association along a longer DNA target. CPs complexed at distances greater than R o from the FL acceptor will be less efficient donors to the PNA-FL probe (red and green).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: SNP detection using peptide nucleic acid probes and conjugated polymers: Applications in neurodegenerative disease identification

    doi: 10.1073/pnas.0407578101

    Figure Lengend Snippet: Cationic CPs (blue) have the ability to associate nonspecifically along any region of a targeted ssDNA sequence (black). ( A ) Association of a short DNA target with the CP. ( B ) The probability of CP association along a longer DNA target. CPs complexed at distances greater than R o from the FL acceptor will be less efficient donors to the PNA-FL probe (red and green).

    Article Snippet: Concentrations of the larger DNA sequences obtained by PCR and gel purification were measured by fluorescence using an OliGreen single stranded DNA (ssDNA) Quantitation kit (Molecular Probes).

    Techniques: Sequencing

    Fluorescence of PNA-FL annealed to complementary 28 (black, D0) and 249 (red, WT) base ssDNA targets ([PNA/ssDNA] = 1 × 10 -8 M) upon addition and excitation (λ ex = 380 nm) of the CP ([CP] = 2 × 10 -7 M) in 30 mM potassium phosphate buffer, pH 7.4. The noncomplementary (28 base, D3) DNA target is shown in blue for reference. The arrow indicates the drop in the relative FL-to-CP emission peak ratio between the two different DNA target lengths.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: SNP detection using peptide nucleic acid probes and conjugated polymers: Applications in neurodegenerative disease identification

    doi: 10.1073/pnas.0407578101

    Figure Lengend Snippet: Fluorescence of PNA-FL annealed to complementary 28 (black, D0) and 249 (red, WT) base ssDNA targets ([PNA/ssDNA] = 1 × 10 -8 M) upon addition and excitation (λ ex = 380 nm) of the CP ([CP] = 2 × 10 -7 M) in 30 mM potassium phosphate buffer, pH 7.4. The noncomplementary (28 base, D3) DNA target is shown in blue for reference. The arrow indicates the drop in the relative FL-to-CP emission peak ratio between the two different DNA target lengths.

    Article Snippet: Concentrations of the larger DNA sequences obtained by PCR and gel purification were measured by fluorescence using an OliGreen single stranded DNA (ssDNA) Quantitation kit (Molecular Probes).

    Techniques: Fluorescence

    Bland-Altman Plot of Difference (Qubit-Nanodrop) (ng/μL) vs. average DNA yield (mean of Qubit and Nanodrop).

    Journal: Biopreservation and Biobanking

    Article Title: Maintaining Breast Cancer Specimen Integrity and Individual or Simultaneous Extraction of Quality DNA, RNA, and Proteins from Allprotect-Stabilized and Nonstabilized Tissue Samples

    doi: 10.1089/bio.2011.0034

    Figure Lengend Snippet: Bland-Altman Plot of Difference (Qubit-Nanodrop) (ng/μL) vs. average DNA yield (mean of Qubit and Nanodrop).

    Article Snippet: DNA was quantified using Qubit [also known as Quant-iT™ dsDNA HS (High Sensitivity) Assay Kit; Invitrogen], Nanodrop 2000™, Thermo Scientific (designated Nanodrop1), and NanoDrop ND-1000™, NanoDrop Technologies (designated Nanodrop2), as per manufacturer's instructions.

    Techniques:

    (A, B) Qubit DNA yields for fresh frozen tumor tissue (*), Fresh frozen normal tissue (**), Allprotect-treated tumor tissue, and Allprotect-treated normal tissue isolated using (A) Qiagen's QIAamp kit (B) Qiagen's AllPrep kit.

    Journal: Biopreservation and Biobanking

    Article Title: Maintaining Breast Cancer Specimen Integrity and Individual or Simultaneous Extraction of Quality DNA, RNA, and Proteins from Allprotect-Stabilized and Nonstabilized Tissue Samples

    doi: 10.1089/bio.2011.0034

    Figure Lengend Snippet: (A, B) Qubit DNA yields for fresh frozen tumor tissue (*), Fresh frozen normal tissue (**), Allprotect-treated tumor tissue, and Allprotect-treated normal tissue isolated using (A) Qiagen's QIAamp kit (B) Qiagen's AllPrep kit.

    Article Snippet: DNA was quantified using Qubit [also known as Quant-iT™ dsDNA HS (High Sensitivity) Assay Kit; Invitrogen], Nanodrop 2000™, Thermo Scientific (designated Nanodrop1), and NanoDrop ND-1000™, NanoDrop Technologies (designated Nanodrop2), as per manufacturer's instructions.

    Techniques: Isolation