quant it picogreen assay kit Thermo Fisher Search Results


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    Thermo Fisher quant it picogreen dsdna assay kit thermo fisher scientific
    Quant It Picogreen Dsdna Assay Kit Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it picogreen dsdna assay kit
    (a) Enhancement of hMSC proliferation using US. MSCs were grown on glass coverslips for 4 days in M2 medium with and without US (14 kPa; 3 min; 1, 2, or 4 times/day). Cells were fixed and stained for Ki67 (mitosis marker) and Hoechst (nuclear marker). Five pictures were randomly taken on three coverslips per condition ( n = 15), and Ki67 and Hoechst positive cells were counted using ImageJ ™ . Data were expressed as percent Ki67 positive. (b) hMSC proliferation is increased with US application. hMSC-seeded scaffolds were cultured in CDM for 14 days and in M3 media for another 7 days in the US-assisted bioreactor according to the culture conditions outlined in Table 2 . <t>dsDNA</t> was assayed by <t>Picogreen</t> assay. Data were presented as average ± standard deviation ( n = 5–8 constructs). Statistically significant data were accounted with respect to respective control and shown as * p
    Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10071 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it picogreen dsdna reagent
    HuR is required for short-term proliferation of PDA cells Relative proliferation of DOX-inducible MIA PaCa-2 cell lines treated with 0 or 2 μg/ml DOX for the indicated time points, as determined by measurement of <t>dsDNA</t> content by <t>PicoGreen</t> staining. Each data point represents the mean of 5 independent experiments ± standard error of the mean (SEM). * = p
    Quant It Picogreen Dsdna Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1939 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it picogreen kit
    HuR is required for short-term proliferation of PDA cells Relative proliferation of DOX-inducible MIA PaCa-2 cell lines treated with 0 or 2 μg/ml DOX for the indicated time points, as determined by measurement of <t>dsDNA</t> content by <t>PicoGreen</t> staining. Each data point represents the mean of 5 independent experiments ± standard error of the mean (SEM). * = p
    Quant It Picogreen Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it dsdna assay
    Morphology, proliferation and differentiation of <t>BMSCs</t> on the MPZs and control surfaces. a) Quant-iT <t>dsDNA</t> Assay of BMSCs on the samples. The assay was repeated twice and expressed as means ± s.d.. Significant differences were determined using a one-way analysis of variance (ANOVA) followed by LSD-t test. (**) and (*) indicates a statisctic difference at p
    Quant It Dsdna Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it picogreen dsdna kit
    Cell proliferation and migration through a 3D-jellyfish collagen scaffold. (A) SKOV.3 and OVCAR.3 cells proliferation rate on 3D jellyfish scaffold from day 2 to 6 assessed through DNA quantification using <t>PicoGreen®</t> <t>dsDNA</t> quantitation assay. (B) SKOV.3 cells were seeded on the top of the scaffold and they migrated and colonized the entire scaffold to the bottom from day 4 to 14. (C) High magnification of SKOV.3 cells directly interacting with collagen as single cells. (D) OVCAR.3 cells were seeded on the top of the scaffold and they migrated and colonized the entire scaffold to the bottom from day 4 to 14. (E) High magnification of OVCAR.3 cells directly interacting with collagen as cluster of cells. Scale bars indicate 100 um.
    Quant It Picogreen Dsdna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1589 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher picogreen quant it dna quantification a picogreen quant it kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Picogreen Quant It Dna Quantification A Picogreen Quant It Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quan it picogreen kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quan It Picogreen Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant ittm picogreen dsdna assay kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quant Ittm Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 500 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant ittm picogreen quantification kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quant Ittm Picogreen Quantification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher picogreen dye solution
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Picogreen Dye Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ribogreen rna assay kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Ribogreen Rna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher picogreen quantitation
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Picogreen Quantitation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fluorometric kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Fluorometric Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it dsdna broad range assay kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quant It Dsdna Broad Range Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fluorescent stain based kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Fluorescent Stain Based Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it picogreen cdna quantification kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quant It Picogreen Cdna Quantification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quan it picogreen double stranded dna dsdna assay kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quan It Picogreen Double Stranded Dna Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quanti it picogreen double stranded dna kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quanti It Picogreen Double Stranded Dna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher probe quant ittm picogreen kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Probe Quant Ittm Picogreen Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher picogreen kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Picogreen Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher picogreen dsdna assay kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1043 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it picogreen dsdna kit proliferation assay
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quant It Picogreen Dsdna Kit Proliferation Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher stain based kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Stain Based Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ethanol te0 1 buffer
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
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    Thermo Fisher quantus picogreen ds dna kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quantus Picogreen Ds Dna Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher picogreen dsdna dye kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Picogreen Dsdna Dye Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant it microrna assay kits
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quant It Microrna Assay Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher double stranded dna detection kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Double Stranded Dna Detection Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quant ittm picogreen r ds dna assay kit
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Quant Ittm Picogreen R Ds Dna Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cell dna concentration
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Cell Dna Concentration, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher picogreen dye
    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free <t>DNA</t> using Quant-iT <t>PicoGreen</t> assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.
    Picogreen Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 600 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/picogreen dye/product/Thermo Fisher
    Average 99 stars, based on 600 article reviews
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    picogreen dye - by Bioz Stars, 2020-02
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    Image Search Results


    (a) Enhancement of hMSC proliferation using US. MSCs were grown on glass coverslips for 4 days in M2 medium with and without US (14 kPa; 3 min; 1, 2, or 4 times/day). Cells were fixed and stained for Ki67 (mitosis marker) and Hoechst (nuclear marker). Five pictures were randomly taken on three coverslips per condition ( n = 15), and Ki67 and Hoechst positive cells were counted using ImageJ ™ . Data were expressed as percent Ki67 positive. (b) hMSC proliferation is increased with US application. hMSC-seeded scaffolds were cultured in CDM for 14 days and in M3 media for another 7 days in the US-assisted bioreactor according to the culture conditions outlined in Table 2 . dsDNA was assayed by Picogreen assay. Data were presented as average ± standard deviation ( n = 5–8 constructs). Statistically significant data were accounted with respect to respective control and shown as * p

    Journal: Journal of Tissue Engineering

    Article Title: Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis

    doi: 10.1177/2041731414566529

    Figure Lengend Snippet: (a) Enhancement of hMSC proliferation using US. MSCs were grown on glass coverslips for 4 days in M2 medium with and without US (14 kPa; 3 min; 1, 2, or 4 times/day). Cells were fixed and stained for Ki67 (mitosis marker) and Hoechst (nuclear marker). Five pictures were randomly taken on three coverslips per condition ( n = 15), and Ki67 and Hoechst positive cells were counted using ImageJ ™ . Data were expressed as percent Ki67 positive. (b) hMSC proliferation is increased with US application. hMSC-seeded scaffolds were cultured in CDM for 14 days and in M3 media for another 7 days in the US-assisted bioreactor according to the culture conditions outlined in Table 2 . dsDNA was assayed by Picogreen assay. Data were presented as average ± standard deviation ( n = 5–8 constructs). Statistically significant data were accounted with respect to respective control and shown as * p

    Article Snippet: Double-stranded DNA (dsDNA) was measured using Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) according to manufacturer’s instructions.

    Techniques: Staining, Marker, Cell Culture, Picogreen Assay, Standard Deviation, Construct

    Viability and proliferation of human GEnCs cultured on collagen I hydrogels and kidney dECM hydrogel substrates (A-C) or encapsulated within hydrogels (D-F). (A) Live/dead staining and confocal imaging of GEnCs cultured on collagen I hydrogels or kidney dECM hydrogels at days 1 and 12. (B) Quantification of viability of cells cultured on top of hydrogel substrates presented as the percentage live cells from image analysis. (C) Quantification of cultured GEnC proliferation over twelve days using a Quant-iT PicoGreen dsDNA Assay Kit. Normalized values (right axis) were normalized to the number of cells seeded per sample on day 0. Samples initially seeded with 12,500 cells per sample (50,000 cells/mL). Number of independent measurements, n = 4. (D) Live/dead staining and confocal imaging of GEnCs encapsulated within collagen I hydrogels or kidney dECM hydrogels at days 1 and 12. (E) Quantification of viability of cells encapsulated within hydrogels presented as the percentage live cells from image analysis. (F) Quantification of encapsulated GEnC proliferation over twelve days using a Quant-iT PicoGreen dsDNA Assay Kit. Normalized values (right axis) were normalized to the number of cells encapsulated per sample on day 0. Samples initially seeded with 150,000 cells per sample (1 million cells/mL). Number of independent measurements, n = 4. *** p

    Journal: Journal of biomedical materials research. Part A

    Article Title: Kidney Decellularized Extracellular Matrix Hydrogels: Rheological Characterization and Human Glomerular Endothelial Cell Response to Encapsulation

    doi: 10.1002/jbm.a.36439

    Figure Lengend Snippet: Viability and proliferation of human GEnCs cultured on collagen I hydrogels and kidney dECM hydrogel substrates (A-C) or encapsulated within hydrogels (D-F). (A) Live/dead staining and confocal imaging of GEnCs cultured on collagen I hydrogels or kidney dECM hydrogels at days 1 and 12. (B) Quantification of viability of cells cultured on top of hydrogel substrates presented as the percentage live cells from image analysis. (C) Quantification of cultured GEnC proliferation over twelve days using a Quant-iT PicoGreen dsDNA Assay Kit. Normalized values (right axis) were normalized to the number of cells seeded per sample on day 0. Samples initially seeded with 12,500 cells per sample (50,000 cells/mL). Number of independent measurements, n = 4. (D) Live/dead staining and confocal imaging of GEnCs encapsulated within collagen I hydrogels or kidney dECM hydrogels at days 1 and 12. (E) Quantification of viability of cells encapsulated within hydrogels presented as the percentage live cells from image analysis. (F) Quantification of encapsulated GEnC proliferation over twelve days using a Quant-iT PicoGreen dsDNA Assay Kit. Normalized values (right axis) were normalized to the number of cells encapsulated per sample on day 0. Samples initially seeded with 150,000 cells per sample (1 million cells/mL). Number of independent measurements, n = 4. *** p

    Article Snippet: DNA was quantified with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, #P7589) following the manufacturer’s protocol and a dsDNA standard included in the kit to generate a standard curve.

    Techniques: Cell Culture, Staining, Imaging, Picogreen Assay

    Inhibition of MRE11 exonuclease activity blocks the expression of innate immune response genes in RAD51-depleted cells. (A and B ) The quantification of ssDNA and dsDNA DNA in the cytosol. RAD51-proficient and -depleted cells were pre-treated with mirin (25 μM), irradiated with 2 Gy and harvested 8 h after irradiation. Subsequently, cells were subjected to sub-cellular fractionation and the amount of cytosolic ssDNA ( A ) and dsDNA ( B ) were quantified using OliGreen and PicoGreen Quant-iT reagents, respectively. The bars represent the changes in the cytoplasmic DNA concentration relative to respective mock-treated samples. Error bars represent STDEV from four independent experiments; *** P

    Journal: Nucleic Acids Research

    Article Title: RAD51 interconnects between DNA replication, DNA repair and immunity

    doi: 10.1093/nar/gkx126

    Figure Lengend Snippet: Inhibition of MRE11 exonuclease activity blocks the expression of innate immune response genes in RAD51-depleted cells. (A and B ) The quantification of ssDNA and dsDNA DNA in the cytosol. RAD51-proficient and -depleted cells were pre-treated with mirin (25 μM), irradiated with 2 Gy and harvested 8 h after irradiation. Subsequently, cells were subjected to sub-cellular fractionation and the amount of cytosolic ssDNA ( A ) and dsDNA ( B ) were quantified using OliGreen and PicoGreen Quant-iT reagents, respectively. The bars represent the changes in the cytoplasmic DNA concentration relative to respective mock-treated samples. Error bars represent STDEV from four independent experiments; *** P

    Article Snippet: Quantification of cytosolic single-strand (ssDNA) and double-strand (dsDNA) DNA ssDNA and dsDNA in the cytosolic fractions were quantified using Quant-iT OliGreen and PicoGreen Assay Kits (O11492 and P7589 kits, respectively; Invitrogen).

    Techniques: Inhibition, Activity Assay, Expressing, Irradiation, Cell Fractionation, Concentration Assay

    Excessive DNA accumulates in the cytosol of RAD51-depleted cells. (A and B) Quantification of single-strand (ssDNA) and double-strand (dsDNA) DNA in the cytosol (cyto): RAD51-proficient and -depleted HT1080 cells were irradiated with 2 Gy and were harvested at the indicated times. Subsequently, cells underwent sub-cellular fractionation and the amount of cytosolic ssDNA ( A ) and dsDNA ( B ) were quantified using OliGreen and PicoGreen Quant-iT reagents, respectively. Bars represent fold changes in the cytoplasmic DNA concentration relative to RAD51-proficient mock-treated samples. Error bars represent the SEM from four independent experiments; ** P

    Journal: Nucleic Acids Research

    Article Title: RAD51 interconnects between DNA replication, DNA repair and immunity

    doi: 10.1093/nar/gkx126

    Figure Lengend Snippet: Excessive DNA accumulates in the cytosol of RAD51-depleted cells. (A and B) Quantification of single-strand (ssDNA) and double-strand (dsDNA) DNA in the cytosol (cyto): RAD51-proficient and -depleted HT1080 cells were irradiated with 2 Gy and were harvested at the indicated times. Subsequently, cells underwent sub-cellular fractionation and the amount of cytosolic ssDNA ( A ) and dsDNA ( B ) were quantified using OliGreen and PicoGreen Quant-iT reagents, respectively. Bars represent fold changes in the cytoplasmic DNA concentration relative to RAD51-proficient mock-treated samples. Error bars represent the SEM from four independent experiments; ** P

    Article Snippet: Quantification of cytosolic single-strand (ssDNA) and double-strand (dsDNA) DNA ssDNA and dsDNA in the cytosolic fractions were quantified using Quant-iT OliGreen and PicoGreen Assay Kits (O11492 and P7589 kits, respectively; Invitrogen).

    Techniques: Irradiation, Cell Fractionation, Concentration Assay

    HuR is required for short-term proliferation of PDA cells Relative proliferation of DOX-inducible MIA PaCa-2 cell lines treated with 0 or 2 μg/ml DOX for the indicated time points, as determined by measurement of dsDNA content by PicoGreen staining. Each data point represents the mean of 5 independent experiments ± standard error of the mean (SEM). * = p

    Journal: Oncotarget

    Article Title: Targeting the mRNA-binding protein HuR impairs malignant characteristics of pancreatic ductal adenocarcinoma cells

    doi:

    Figure Lengend Snippet: HuR is required for short-term proliferation of PDA cells Relative proliferation of DOX-inducible MIA PaCa-2 cell lines treated with 0 or 2 μg/ml DOX for the indicated time points, as determined by measurement of dsDNA content by PicoGreen staining. Each data point represents the mean of 5 independent experiments ± standard error of the mean (SEM). * = p

    Article Snippet: Double-stranded DNA (dsDNA) was stained by Quant-iT PicoGreen dsDNA reagent (Life Technologies, cat. #P7581), and fluorescence intensity was measured by a microplate reader (Tecan, part #F129015) using excitation wavelength of 485 nm and emission wavelength of 535 nm.

    Techniques: Staining

    Morphology, proliferation and differentiation of BMSCs on the MPZs and control surfaces. a) Quant-iT dsDNA Assay of BMSCs on the samples. The assay was repeated twice and expressed as means ± s.d.. Significant differences were determined using a one-way analysis of variance (ANOVA) followed by LSD-t test. (**) and (*) indicates a statisctic difference at p

    Journal: Theranostics

    Article Title: Bone-Inspired Spatially Specific Piezoelectricity Induces Bone Regeneration

    doi: 10.7150/thno.19748

    Figure Lengend Snippet: Morphology, proliferation and differentiation of BMSCs on the MPZs and control surfaces. a) Quant-iT dsDNA Assay of BMSCs on the samples. The assay was repeated twice and expressed as means ± s.d.. Significant differences were determined using a one-way analysis of variance (ANOVA) followed by LSD-t test. (**) and (*) indicates a statisctic difference at p

    Article Snippet: Proliferation of BMSCs was determined using Quant-iT dsDNA Assay (PicoGreen, Invitrogen, USA).

    Techniques: dsDNA Assay

    Cell proliferation and migration through a 3D-jellyfish collagen scaffold. (A) SKOV.3 and OVCAR.3 cells proliferation rate on 3D jellyfish scaffold from day 2 to 6 assessed through DNA quantification using PicoGreen® dsDNA quantitation assay. (B) SKOV.3 cells were seeded on the top of the scaffold and they migrated and colonized the entire scaffold to the bottom from day 4 to 14. (C) High magnification of SKOV.3 cells directly interacting with collagen as single cells. (D) OVCAR.3 cells were seeded on the top of the scaffold and they migrated and colonized the entire scaffold to the bottom from day 4 to 14. (E) High magnification of OVCAR.3 cells directly interacting with collagen as cluster of cells. Scale bars indicate 100 um.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Marine Collagen Substrates for 2D and 3D Ovarian Cancer Cell Systems

    doi: 10.3389/fbioe.2019.00343

    Figure Lengend Snippet: Cell proliferation and migration through a 3D-jellyfish collagen scaffold. (A) SKOV.3 and OVCAR.3 cells proliferation rate on 3D jellyfish scaffold from day 2 to 6 assessed through DNA quantification using PicoGreen® dsDNA quantitation assay. (B) SKOV.3 cells were seeded on the top of the scaffold and they migrated and colonized the entire scaffold to the bottom from day 4 to 14. (C) High magnification of SKOV.3 cells directly interacting with collagen as single cells. (D) OVCAR.3 cells were seeded on the top of the scaffold and they migrated and colonized the entire scaffold to the bottom from day 4 to 14. (E) High magnification of OVCAR.3 cells directly interacting with collagen as cluster of cells. Scale bars indicate 100 um.

    Article Snippet: A buffer made up of 300 ug/ml of papain, 2 mM DTT, 20 mM NaAc ph 6.8, 1 mM EDTA was used to incubate the sample at 60 degree for up to 2 h. Quant-iT picogreen dsDNA kit (Invitrogen) was used to assess double-stranded DNA in solution.

    Techniques: Migration, Quantitation Assay

    Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free DNA using Quant-iT PicoGreen assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.

    Journal: PLoS ONE

    Article Title: Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis

    doi: 10.1371/journal.pone.0148197

    Figure Lengend Snippet: Serum levels of NETs in AAV patients in active stage and remission. A. Quantification of cell free DNA using Quant-iT PicoGreen assay in serum samples from healthy donors (n = 22), individuals with AAV in remission (n = 62) and with active disease (n = 34). B. Quantification of NETs in the serum samples by MPO-DNA complex ELISA. The mean optical density as measured by capture ELISA.

    Article Snippet: As an indirect way to assess NETs, cell free DNA in serum was quantified using Quant-iT PicoGreen DNA quantification kit (Invitrogen) in accordance with the manufacturer’s instructions.

    Techniques: Picogreen Assay, Enzyme-linked Immunosorbent Assay