quality control dna cs Search Results


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  • 99
    New England Biolabs nebnext end repair module
    Nebnext End Repair Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1374 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC k pneumoniae atcc 35657
    K Pneumoniae Atcc 35657, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiaamp dna mini kit
    Qiaamp Dna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 48891 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 3730 dna analyzer
    3730 Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8730 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MACHEREY NAGEL dna extraction dna extraction
    Dna Extraction Dna Extraction, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Elan Drug Technologies 137 cs blood irradiator
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    137 Cs Blood Irradiator, supplied by Elan Drug Technologies, used in various techniques. Bioz Stars score: 91/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MACHEREY NAGEL nucleospin soil dna isolation kit
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    Nucleospin Soil Dna Isolation Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 88/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore taq dna polymerase
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    Taq Dna Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3580 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc dna methylation analysis
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    Dna Methylation Analysis, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 873 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sysmex Corporation xn check material
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    Xn Check Material, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC candida albicans
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    Candida Albicans, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2772 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs end repair reaction buffer
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    End Repair Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rt2 rna qc pcr arrays
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    Rt2 Rna Qc Pcr Arrays, supplied by Qiagen, used in various techniques. Bioz Stars score: 88/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc ddrad libraries
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    Ddrad Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher trizol reagent
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 635164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sysmex Corporation xe 5000 automated analyzer
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    Xe 5000 Automated Analyzer, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa moloney murine leukemia virus m mlv reverse transcriptase
    <t>137</t> Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P
    Moloney Murine Leukemia Virus M Mlv Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti calreticulin crt antibody
    Wogonin induced <t>calreticulin</t> <t>(CRT)</t> translocation to cell surface membrane is dependent on the PERK and PI3K/AKT. Gastric carcinoma cell line MFC cells were transfected with scramble siRNA (Ctrl, 200 nM) or PERK siRNA (200 nM), after 48 hours, expression level of PERK was detected by Western blots to verify PERK level after siRNA treatment. Successfully PERK knockdown cells and their control cells (Ctrl siRNA treated cells) were treated with wogonin (100 µM) for 2 and 4 hours. Cell surface proteins in the plasma membrane fraction were than biotinylated and tested for CRT, EGFR and actin. Total cell lysate were also obtained to test CRT, PERK and actin (A). MFC cells were pretreated with AKT specific inhibitor X (AKTi 100 nM) or PI3K/AKT inhibitor LY294002 (100 nM) for 2 hours, followed by wogonin (100 µM) treated for 2 and 4 hours, CRT, EGFR and actin in the plasma membrane protein fraction were detected by Western blots. CRT, p-AKT (ser 473), AKT1/2 and actin in total cell lysate were also detected by Western blots (B).CRT translocation to cell surface after wogonin treatment was also confirmed by confocal immune-fluorescence microscopy, the translocation was inhibited by Akt inhibitor X (AKTi 100 nM), doxorubicin (Dox, 1 µM, 2 hrs treatment) was used here as positive controls. (C). WT and AKT1/2 double knockout MEFs were treated with wogonin (100 µM) for 4 hours; CRT, EGFR and actin in the plasma membrane protein fraction were detected by Western blots. P-S6 (S235/236), p-AKT (S473), AKT1/2, CRT and actin in whole cell lysate were detected by Western blots(D). Experiments in this figure were repeated at least 3 times and similar results were obtained. Bar = 10 µm.
    Anti Calreticulin Crt Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc illumina infinium
    Wogonin induced <t>calreticulin</t> <t>(CRT)</t> translocation to cell surface membrane is dependent on the PERK and PI3K/AKT. Gastric carcinoma cell line MFC cells were transfected with scramble siRNA (Ctrl, 200 nM) or PERK siRNA (200 nM), after 48 hours, expression level of PERK was detected by Western blots to verify PERK level after siRNA treatment. Successfully PERK knockdown cells and their control cells (Ctrl siRNA treated cells) were treated with wogonin (100 µM) for 2 and 4 hours. Cell surface proteins in the plasma membrane fraction were than biotinylated and tested for CRT, EGFR and actin. Total cell lysate were also obtained to test CRT, PERK and actin (A). MFC cells were pretreated with AKT specific inhibitor X (AKTi 100 nM) or PI3K/AKT inhibitor LY294002 (100 nM) for 2 hours, followed by wogonin (100 µM) treated for 2 and 4 hours, CRT, EGFR and actin in the plasma membrane protein fraction were detected by Western blots. CRT, p-AKT (ser 473), AKT1/2 and actin in total cell lysate were also detected by Western blots (B).CRT translocation to cell surface after wogonin treatment was also confirmed by confocal immune-fluorescence microscopy, the translocation was inhibited by Akt inhibitor X (AKTi 100 nM), doxorubicin (Dox, 1 µM, 2 hrs treatment) was used here as positive controls. (C). WT and AKT1/2 double knockout MEFs were treated with wogonin (100 µM) for 4 hours; CRT, EGFR and actin in the plasma membrane protein fraction were detected by Western blots. P-S6 (S235/236), p-AKT (S473), AKT1/2, CRT and actin in whole cell lysate were detected by Western blots(D). Experiments in this figure were repeated at least 3 times and similar results were obtained. Bar = 10 µm.
    Illumina Infinium, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 1313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    137 Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P

    Journal: PLoS ONE

    Article Title: Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

    doi: 10.1371/journal.pone.0127181

    Figure Lengend Snippet: 137 Cs gamma-ray irradiation inhibits the colony formation by tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 14 d, and then the colony formation was detected by Giemsa staining. Representative Giemsa staining (scale bar: 100 μm) of HepG2, SW620 and SGC7901 cells 7 d after irradiation (A). Colony formation rate of tumor cells at 14 d after irradiation (B). Data are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. aa P

    Article Snippet: In conclusion, our present study suggested that 50 Gy irradiation given by a standard 137 Cs blood irradiator is a safe and effective method to inactivate HepG2, SGC7901, and SW620 cells mixed with erythrocytes while preserving the quality of erythrocytes.

    Techniques: Irradiation, In Vitro, Cell Culture, Staining

    Effects of 137 Cs gamma-ray irradiation on ROS levels and SOD in erythrocytes. After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), the ROS level (A) and SOD (B) in erythrocytes were determined by the fluorescence probe CM-H 2 -DCFDA and a commercial kit, respectively. Con: the dose of irradiation was 0 Gy; H 2 O 2 : positive control group. n = 14 (B) SOD of RBCs change after irradiation. Data are means ± SEM, n = 14.

    Journal: PLoS ONE

    Article Title: Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

    doi: 10.1371/journal.pone.0127181

    Figure Lengend Snippet: Effects of 137 Cs gamma-ray irradiation on ROS levels and SOD in erythrocytes. After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), the ROS level (A) and SOD (B) in erythrocytes were determined by the fluorescence probe CM-H 2 -DCFDA and a commercial kit, respectively. Con: the dose of irradiation was 0 Gy; H 2 O 2 : positive control group. n = 14 (B) SOD of RBCs change after irradiation. Data are means ± SEM, n = 14.

    Article Snippet: In conclusion, our present study suggested that 50 Gy irradiation given by a standard 137 Cs blood irradiator is a safe and effective method to inactivate HepG2, SGC7901, and SW620 cells mixed with erythrocytes while preserving the quality of erythrocytes.

    Techniques: Irradiation, Fluorescence, Positive Control

    Effects of 137 Cs gamma-ray irradiation on erythrocytes in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), ATP (A), 2,3-DPG (B), free Hb (C) and osmotic fragility (D) in erythrocytes were determined. Data are means ± SEM; n = 14 erythrocyte samples from 14 volunteers in each group. aa P

    Journal: PLoS ONE

    Article Title: Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

    doi: 10.1371/journal.pone.0127181

    Figure Lengend Snippet: Effects of 137 Cs gamma-ray irradiation on erythrocytes in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), ATP (A), 2,3-DPG (B), free Hb (C) and osmotic fragility (D) in erythrocytes were determined. Data are means ± SEM; n = 14 erythrocyte samples from 14 volunteers in each group. aa P

    Article Snippet: In conclusion, our present study suggested that 50 Gy irradiation given by a standard 137 Cs blood irradiator is a safe and effective method to inactivate HepG2, SGC7901, and SW620 cells mixed with erythrocytes while preserving the quality of erythrocytes.

    Techniques: Irradiation, In Vitro

    137 Cs gamma-ray irradiation inhibits DNA synthesis in tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 24 h, and then DNA synthesis was detected by 5-ethynyl-2'-deoxyuridine (EdU) incorporation. Representative DNA synthesis (scale bar: 200 μm) of HepG2 cells at 24 h after irradiation with 0, 30, 50 and 100 Gy (A). DNA synthesis in HepG2 (B), SW620 (C) and SGC7901 (D) cells at 24 h and 72 h after irradiation. Date are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. ** P

    Journal: PLoS ONE

    Article Title: Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

    doi: 10.1371/journal.pone.0127181

    Figure Lengend Snippet: 137 Cs gamma-ray irradiation inhibits DNA synthesis in tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were cultured for 24 h, and then DNA synthesis was detected by 5-ethynyl-2'-deoxyuridine (EdU) incorporation. Representative DNA synthesis (scale bar: 200 μm) of HepG2 cells at 24 h after irradiation with 0, 30, 50 and 100 Gy (A). DNA synthesis in HepG2 (B), SW620 (C) and SGC7901 (D) cells at 24 h and 72 h after irradiation. Date are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. ** P

    Article Snippet: In conclusion, our present study suggested that 50 Gy irradiation given by a standard 137 Cs blood irradiator is a safe and effective method to inactivate HepG2, SGC7901, and SW620 cells mixed with erythrocytes while preserving the quality of erythrocytes.

    Techniques: Irradiation, DNA Synthesis, In Vitro, Cell Culture

    137 Cs gamma-ray irradiation inhibits the viability of tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2 (A), SW620 (B), and SGC7901 (C) cells separated from human erythrocytes were cultured for 24 h, (D) 48 h and 72 h, and then the viability was detected by MTT assay. The cell viability was indicated by the percentage of viable cells. Date are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. Con: the dose of irradiation was 0. * P

    Journal: PLoS ONE

    Article Title: Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

    doi: 10.1371/journal.pone.0127181

    Figure Lengend Snippet: 137 Cs gamma-ray irradiation inhibits the viability of tumor cell lines in vitro . After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2 (A), SW620 (B), and SGC7901 (C) cells separated from human erythrocytes were cultured for 24 h, (D) 48 h and 72 h, and then the viability was detected by MTT assay. The cell viability was indicated by the percentage of viable cells. Date are means ± SEM; n = 6 erythrocyte samples from 6 volunteers in each group. Con: the dose of irradiation was 0. * P

    Article Snippet: In conclusion, our present study suggested that 50 Gy irradiation given by a standard 137 Cs blood irradiator is a safe and effective method to inactivate HepG2, SGC7901, and SW620 cells mixed with erythrocytes while preserving the quality of erythrocytes.

    Techniques: Irradiation, In Vitro, Cell Culture, MTT Assay

    137 Cs gamma-ray irradiation induces apoptosis and necrosis in tumor cell lines in vitro . Immediately after 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), apoptosis and necrosis in HepG2, SW620 and SGC7901 cells separated from human erythrocytes was determined by annexin V/7-AAD staining.

    Journal: PLoS ONE

    Article Title: Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

    doi: 10.1371/journal.pone.0127181

    Figure Lengend Snippet: 137 Cs gamma-ray irradiation induces apoptosis and necrosis in tumor cell lines in vitro . Immediately after 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), apoptosis and necrosis in HepG2, SW620 and SGC7901 cells separated from human erythrocytes was determined by annexin V/7-AAD staining.

    Article Snippet: In conclusion, our present study suggested that 50 Gy irradiation given by a standard 137 Cs blood irradiator is a safe and effective method to inactivate HepG2, SGC7901, and SW620 cells mixed with erythrocytes while preserving the quality of erythrocytes.

    Techniques: Irradiation, In Vitro, Staining

    137 Cs gamma-ray irradiation induces death in HepG2 cells in vitro . After 0 (A), 30 (B), 50 (C) and 100 (D) Gy 137 Cs gamma-ray irradiation, HepG2 cells separated from human erythrocytes were cultured for 7 d, and then their morphology (scale bar: 40 μm) was observed.

    Journal: PLoS ONE

    Article Title: Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

    doi: 10.1371/journal.pone.0127181

    Figure Lengend Snippet: 137 Cs gamma-ray irradiation induces death in HepG2 cells in vitro . After 0 (A), 30 (B), 50 (C) and 100 (D) Gy 137 Cs gamma-ray irradiation, HepG2 cells separated from human erythrocytes were cultured for 7 d, and then their morphology (scale bar: 40 μm) was observed.

    Article Snippet: In conclusion, our present study suggested that 50 Gy irradiation given by a standard 137 Cs blood irradiator is a safe and effective method to inactivate HepG2, SGC7901, and SW620 cells mixed with erythrocytes while preserving the quality of erythrocytes.

    Techniques: Irradiation, In Vitro, Cell Culture

    Effects of 137 Cs gamma-ray irradiation on phosphatidylserine exposure in erythrocytic membranes. After 137 Cs gamma-ray irradiation (0, 50 and 100 Gy), phosphatidylserine exposure in erythrocytic membranes was detected by annexin V staining. Data are means ± SEM; n = 14 erythrocyte samples from 14 volunteers in each group.

    Journal: PLoS ONE

    Article Title: Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

    doi: 10.1371/journal.pone.0127181

    Figure Lengend Snippet: Effects of 137 Cs gamma-ray irradiation on phosphatidylserine exposure in erythrocytic membranes. After 137 Cs gamma-ray irradiation (0, 50 and 100 Gy), phosphatidylserine exposure in erythrocytic membranes was detected by annexin V staining. Data are means ± SEM; n = 14 erythrocyte samples from 14 volunteers in each group.

    Article Snippet: In conclusion, our present study suggested that 50 Gy irradiation given by a standard 137 Cs blood irradiator is a safe and effective method to inactivate HepG2, SGC7901, and SW620 cells mixed with erythrocytes while preserving the quality of erythrocytes.

    Techniques: Irradiation, Staining

    137 Cs gamma-ray irradiation inhibits the growth of xenograft tumors in immunocompromised mice. After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were subcutaneously xenotransplanted into male BALB/c nude mice. The histopathology of xenograft tumors growing up to 2 cm in diameter was detected by H E staining. Representative subcutaneous xenograft tumor and histopathology of xenograft tumor developed by non-irradiated HepG2, SW620 and SGC7901 cells (scale bar: 50 μm) with H E staining (A). The body weights of immunocompromised mice subcutaneously xenotransplanted with HepG2 (B), SW620 (C) and SGC7901 (D) cells. The volume of xenograft tumors developed by HepG2 (E), SW620 (F) and SGC7901 (G) cells subjected to 0 and 30 Gy irradiation in immunocompromised mice. Date are means ± SEM; n = 8 mice in each group. aa P

    Journal: PLoS ONE

    Article Title: Irradiation Can Selectively Kill Tumor Cells while Preserving Erythrocyte Viability in a Co-Culture System

    doi: 10.1371/journal.pone.0127181

    Figure Lengend Snippet: 137 Cs gamma-ray irradiation inhibits the growth of xenograft tumors in immunocompromised mice. After 137 Cs gamma-ray irradiation (0, 30, 50 and 100 Gy), HepG2, SW620 and SGC7901 cells separated from human erythrocytes were subcutaneously xenotransplanted into male BALB/c nude mice. The histopathology of xenograft tumors growing up to 2 cm in diameter was detected by H E staining. Representative subcutaneous xenograft tumor and histopathology of xenograft tumor developed by non-irradiated HepG2, SW620 and SGC7901 cells (scale bar: 50 μm) with H E staining (A). The body weights of immunocompromised mice subcutaneously xenotransplanted with HepG2 (B), SW620 (C) and SGC7901 (D) cells. The volume of xenograft tumors developed by HepG2 (E), SW620 (F) and SGC7901 (G) cells subjected to 0 and 30 Gy irradiation in immunocompromised mice. Date are means ± SEM; n = 8 mice in each group. aa P

    Article Snippet: In conclusion, our present study suggested that 50 Gy irradiation given by a standard 137 Cs blood irradiator is a safe and effective method to inactivate HepG2, SGC7901, and SW620 cells mixed with erythrocytes while preserving the quality of erythrocytes.

    Techniques: Irradiation, Mouse Assay, Histopathology, Staining

    Wogonin induced calreticulin (CRT) translocation to cell surface membrane is dependent on the PERK and PI3K/AKT. Gastric carcinoma cell line MFC cells were transfected with scramble siRNA (Ctrl, 200 nM) or PERK siRNA (200 nM), after 48 hours, expression level of PERK was detected by Western blots to verify PERK level after siRNA treatment. Successfully PERK knockdown cells and their control cells (Ctrl siRNA treated cells) were treated with wogonin (100 µM) for 2 and 4 hours. Cell surface proteins in the plasma membrane fraction were than biotinylated and tested for CRT, EGFR and actin. Total cell lysate were also obtained to test CRT, PERK and actin (A). MFC cells were pretreated with AKT specific inhibitor X (AKTi 100 nM) or PI3K/AKT inhibitor LY294002 (100 nM) for 2 hours, followed by wogonin (100 µM) treated for 2 and 4 hours, CRT, EGFR and actin in the plasma membrane protein fraction were detected by Western blots. CRT, p-AKT (ser 473), AKT1/2 and actin in total cell lysate were also detected by Western blots (B).CRT translocation to cell surface after wogonin treatment was also confirmed by confocal immune-fluorescence microscopy, the translocation was inhibited by Akt inhibitor X (AKTi 100 nM), doxorubicin (Dox, 1 µM, 2 hrs treatment) was used here as positive controls. (C). WT and AKT1/2 double knockout MEFs were treated with wogonin (100 µM) for 4 hours; CRT, EGFR and actin in the plasma membrane protein fraction were detected by Western blots. P-S6 (S235/236), p-AKT (S473), AKT1/2, CRT and actin in whole cell lysate were detected by Western blots(D). Experiments in this figure were repeated at least 3 times and similar results were obtained. Bar = 10 µm.

    Journal: PLoS ONE

    Article Title: Wogonin Induced Calreticulin/Annexin A1 Exposure Dictates the Immunogenicity of Cancer Cells in a PERK/AKT Dependent Manner

    doi: 10.1371/journal.pone.0050811

    Figure Lengend Snippet: Wogonin induced calreticulin (CRT) translocation to cell surface membrane is dependent on the PERK and PI3K/AKT. Gastric carcinoma cell line MFC cells were transfected with scramble siRNA (Ctrl, 200 nM) or PERK siRNA (200 nM), after 48 hours, expression level of PERK was detected by Western blots to verify PERK level after siRNA treatment. Successfully PERK knockdown cells and their control cells (Ctrl siRNA treated cells) were treated with wogonin (100 µM) for 2 and 4 hours. Cell surface proteins in the plasma membrane fraction were than biotinylated and tested for CRT, EGFR and actin. Total cell lysate were also obtained to test CRT, PERK and actin (A). MFC cells were pretreated with AKT specific inhibitor X (AKTi 100 nM) or PI3K/AKT inhibitor LY294002 (100 nM) for 2 hours, followed by wogonin (100 µM) treated for 2 and 4 hours, CRT, EGFR and actin in the plasma membrane protein fraction were detected by Western blots. CRT, p-AKT (ser 473), AKT1/2 and actin in total cell lysate were also detected by Western blots (B).CRT translocation to cell surface after wogonin treatment was also confirmed by confocal immune-fluorescence microscopy, the translocation was inhibited by Akt inhibitor X (AKTi 100 nM), doxorubicin (Dox, 1 µM, 2 hrs treatment) was used here as positive controls. (C). WT and AKT1/2 double knockout MEFs were treated with wogonin (100 µM) for 4 hours; CRT, EGFR and actin in the plasma membrane protein fraction were detected by Western blots. P-S6 (S235/236), p-AKT (S473), AKT1/2, CRT and actin in whole cell lysate were detected by Western blots(D). Experiments in this figure were repeated at least 3 times and similar results were obtained. Bar = 10 µm.

    Article Snippet: Precleared samples were incubated with anti-Calreticulin (CRT) Antibody (cs-2891, Cell Signaling Tech), or anti-DNA-PKcs (sc-9051, Santa Cruz antibodies) in lysis buffer overnight at 4°C.

    Techniques: Translocation Assay, Transfection, Expressing, Western Blot, Fluorescence, Microscopy, Double Knockout