qrt pcr reactions Thermo Fisher Search Results


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  • 99
    Thermo Fisher pcr buffer
    HGF/SF mRNA and protein expression in normal colon mucosa and colorectal carcinomas. A: <t>RT-PCR</t> was performed on total RNA isolated from five pairs of normal colon (N) and primary colorectal carcinoma (T), on water (none), and on a plasmid containing full-length human HGF/SF cDNA (pHGF/SF). Primers used were HGF/SF-specific or, as a control, glyceraldehydephosphate dehydrogenase (GAPDH)-specific. The tumors analyzed were CD44- and Met-positive. B: HGF/SF protein expression in colorectal cancer was assessed by immunohistochemistry. a and b : Frozen sections from colorectal cancer tissue were stained with anti-human HGF/SF. This identified cells ( arrows ) in the tumor stroma as HGF/SF-producing cells.
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 28829 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher qrt polymerase chain reaction pcr
    Primer Design and <t>qRT-PCR</t>
    Qrt Polymerase Chain Reaction Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 361 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher transcription polymerase chain reaction
    Primer Design and <t>qRT-PCR</t>
    Transcription Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reverse transcription polymerase chain reaction qrt pcr
    Primer Design and <t>qRT-PCR</t>
    Reverse Transcription Polymerase Chain Reaction Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 583 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reverse transcriptase polymerase chain reaction qrt pcr
    Primer Design and <t>qRT-PCR</t>
    Reverse Transcriptase Polymerase Chain Reaction Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher transcript polymerase chain reaction qrt pcr kit
    Primer Design and <t>qRT-PCR</t>
    Transcript Polymerase Chain Reaction Qrt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time polymerase chain reaction qrt pcr amplification
    Characterization of apoptosis triggered by TCEA3 expression. a RH30 cells expressing TCEA3 or empty vector control were harvested for RNA. BAX and BCL2 gene expression were assayed by <t>qRT-PCR.</t> Scale bars 50 µm. b RH30 cells expressing TCEA3 or empty vector were assayed by western blot with antibodies against the indicated apoptotic marker proteins. GAPDH was used as a loading control. c RH30 cells expressing TCEA3 and empty vector were treated with caspase 8 inhibitor (Z-IETD-FMK, 40 μM), caspase 9 inhibitor (Z-LEHD-FMK, 40 μM) or a pan-caspase inhibitor (Z-VAD-FMK, 50 μM) for 18 h. Immunofluorescence assay was done with anti TCEA3 antibody (green) and anti-cleaved caspase 3 antibody (red). DAPI (blue) was used to visualize nuclei. Scale bar is 50 µm. d Western blot assay on the same cells as in C. to confirm the protein expression of TCEA3, caspase 3 and tubulin as a loading control. * marks cleaved caspase 3. Error bars are S.E.M. Student t test; *** p
    Real Time Polymerase Chain Reaction Qrt Pcr Amplification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polymerase chain reaction qrt pcr rneasy mini kit
    Characterization of apoptosis triggered by TCEA3 expression. a RH30 cells expressing TCEA3 or empty vector control were harvested for RNA. BAX and BCL2 gene expression were assayed by <t>qRT-PCR.</t> Scale bars 50 µm. b RH30 cells expressing TCEA3 or empty vector were assayed by western blot with antibodies against the indicated apoptotic marker proteins. GAPDH was used as a loading control. c RH30 cells expressing TCEA3 and empty vector were treated with caspase 8 inhibitor (Z-IETD-FMK, 40 μM), caspase 9 inhibitor (Z-LEHD-FMK, 40 μM) or a pan-caspase inhibitor (Z-VAD-FMK, 50 μM) for 18 h. Immunofluorescence assay was done with anti TCEA3 antibody (green) and anti-cleaved caspase 3 antibody (red). DAPI (blue) was used to visualize nuclei. Scale bar is 50 µm. d Western blot assay on the same cells as in C. to confirm the protein expression of TCEA3, caspase 3 and tubulin as a loading control. * marks cleaved caspase 3. Error bars are S.E.M. Student t test; *** p
    Polymerase Chain Reaction Qrt Pcr Rneasy Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time polymerase chain reaction
    Characterization of apoptosis triggered by TCEA3 expression. a RH30 cells expressing TCEA3 or empty vector control were harvested for RNA. BAX and BCL2 gene expression were assayed by <t>qRT-PCR.</t> Scale bars 50 µm. b RH30 cells expressing TCEA3 or empty vector were assayed by western blot with antibodies against the indicated apoptotic marker proteins. GAPDH was used as a loading control. c RH30 cells expressing TCEA3 and empty vector were treated with caspase 8 inhibitor (Z-IETD-FMK, 40 μM), caspase 9 inhibitor (Z-LEHD-FMK, 40 μM) or a pan-caspase inhibitor (Z-VAD-FMK, 50 μM) for 18 h. Immunofluorescence assay was done with anti TCEA3 antibody (green) and anti-cleaved caspase 3 antibody (red). DAPI (blue) was used to visualize nuclei. Scale bar is 50 µm. d Western blot assay on the same cells as in C. to confirm the protein expression of TCEA3, caspase 3 and tubulin as a loading control. * marks cleaved caspase 3. Error bars are S.E.M. Student t test; *** p
    Real Time Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1477 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reverse transcriptase polymerase chain reaction
    Characterization of apoptosis triggered by TCEA3 expression. a RH30 cells expressing TCEA3 or empty vector control were harvested for RNA. BAX and BCL2 gene expression were assayed by <t>qRT-PCR.</t> Scale bars 50 µm. b RH30 cells expressing TCEA3 or empty vector were assayed by western blot with antibodies against the indicated apoptotic marker proteins. GAPDH was used as a loading control. c RH30 cells expressing TCEA3 and empty vector were treated with caspase 8 inhibitor (Z-IETD-FMK, 40 μM), caspase 9 inhibitor (Z-LEHD-FMK, 40 μM) or a pan-caspase inhibitor (Z-VAD-FMK, 50 μM) for 18 h. Immunofluorescence assay was done with anti TCEA3 antibody (green) and anti-cleaved caspase 3 antibody (red). DAPI (blue) was used to visualize nuclei. Scale bar is 50 µm. d Western blot assay on the same cells as in C. to confirm the protein expression of TCEA3, caspase 3 and tubulin as a loading control. * marks cleaved caspase 3. Error bars are S.E.M. Student t test; *** p
    Reverse Transcriptase Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 446 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reverse transcribed polymerase chain reaction
    Characterization of apoptosis triggered by TCEA3 expression. a RH30 cells expressing TCEA3 or empty vector control were harvested for RNA. BAX and BCL2 gene expression were assayed by <t>qRT-PCR.</t> Scale bars 50 µm. b RH30 cells expressing TCEA3 or empty vector were assayed by western blot with antibodies against the indicated apoptotic marker proteins. GAPDH was used as a loading control. c RH30 cells expressing TCEA3 and empty vector were treated with caspase 8 inhibitor (Z-IETD-FMK, 40 μM), caspase 9 inhibitor (Z-LEHD-FMK, 40 μM) or a pan-caspase inhibitor (Z-VAD-FMK, 50 μM) for 18 h. Immunofluorescence assay was done with anti TCEA3 antibody (green) and anti-cleaved caspase 3 antibody (red). DAPI (blue) was used to visualize nuclei. Scale bar is 50 µm. d Western blot assay on the same cells as in C. to confirm the protein expression of TCEA3, caspase 3 and tubulin as a loading control. * marks cleaved caspase 3. Error bars are S.E.M. Student t test; *** p
    Reverse Transcribed Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative real time polymerase chain reaction qrt pcr
    <t>QRT-PCR</t> for 27-hydroxylase and ABCA1 message in NS398-treated THP-1 cells exposed to prostaglandins. (a) 27-Hydroxylase message is decreased by the COX-2 inhibitor NS398 and this decrease is reversed by prostaglandins E 1 , E 2 , and D 2 . THP-1 human monocytes were exposed to the following conditions represented by the six bars (from left to right): (1) RPMI 1640, (2) NS398 (50 μM), (3) PGE 1 (0.1 μM) + NS398 (50 μM), (4) PGE 2 (0.1 μM) + NS398 (50 μM), (5) PGE 1 (0.1 μM) + PGE 2 (0.1 μM) + NS398 (50 μM), and (6) PGD 2 (14 μM) + NS398 (50 μM) (all 18-hour exposures). Cells were extracted for total RNA and were evaluated for 27-hydroxylase mRNA expression by QRT-PCR. Signals obtained from the amplification of GAPDH message were used as internal controls. (b) ABCA1 message is decreased by the COX-2 inhibitor NS398 and this decrease is reversed by prostaglandins E 1 , E 2 , and D 2 . THP-1 human monocytes were exposed to the following conditions represented by the six bars (from left to right): (1) RPMI 1640, (2) NS398 (50 μM), (3) PGE 1 (0.1 μM) + NS398 (50 μM), (4) PGE 2 (0.1 μM) + NS398 (50 μM), (5) PGE 1 (0.1 μM) + PGE 2 (0.1 μM) + NS398 (50 μM), and (6) PGD 2 (14 μM) + NS398 (50 μM) (all 18-hour exposures). Cells were extracted for total RNA and were evaluated for ABCA1 mRNA expression by QRT-PCR. Signals obtained from the amplification of GAPDH message were used as internal controls. ** p
    Quantitative Real Time Polymerase Chain Reaction Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2693 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative reverse transcription polymerase chain reaction qrt pcr
    ( A ) Hierarchal clustering of differentially expressed microRNAs. Forty-six differentially expressed microRNAs (for up- and down-regulation, respectively) compared to the negative control group in at least one sample were clustered hierarchically. Each rows represent individual microRNAs. The expression ratio representing colour ranges from green (low) to red (high), as indicated by the scale bar. The column bar chart shows the relative expression level of selected microRNAs by <t>qRT-PCR</t> (right column, white) as compared to the fold changes of the corresponding microRNA (left column, grey); ( B ) MicroRNAs expression of Group B versus Group A on the first day of treatment; ( C ) Group B versus Group A on the third day of treatment; ( D ) MicroRNAs expression of on the fifth day of treatment. U87 was used as housekeeping gene. Bars represent the mean ± standard error of three independent experiments; and ( E ) qRT-PCR product was confirmed by gel electrophoresis at 90 V for 40 min.
    Quantitative Reverse Transcription Polymerase Chain Reaction Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative real time polymerase chain reaction qrt pcr trizol
    Inhibition of LINC01116 suppressed cell proliferation and migration in NPC cells. A, <t>qRT‐PCR</t> result of LINC01116 expression in NPC cell lines and the normal HNEpC cells. B, qRT‐PCR result of LINC01116 expression in CNE2 and 5‐8F cells under the transfection of shCtrl or two shRNAs against LINC01116. C, CCK‐8 assay was performed to evaluate cell viability in above cells. D, Cell proliferation was examined by EdU assay. E, Transwell assay for the assessment of cell migration in NPC cells with or without LINC01116 inhibition. * P
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    Thermo Fisher quantitative real time polymerase chain reaction qrt pcr assay kits
    Inhibition of LINC01116 suppressed cell proliferation and migration in NPC cells. A, <t>qRT‐PCR</t> result of LINC01116 expression in NPC cell lines and the normal HNEpC cells. B, qRT‐PCR result of LINC01116 expression in CNE2 and 5‐8F cells under the transfection of shCtrl or two shRNAs against LINC01116. C, CCK‐8 assay was performed to evaluate cell viability in above cells. D, Cell proliferation was examined by EdU assay. E, Transwell assay for the assessment of cell migration in NPC cells with or without LINC01116 inhibition. * P
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    Thermo Fisher 7500 real time polymerase chain reaction qrt pcr
    Inhibition of LINC01116 suppressed cell proliferation and migration in NPC cells. A, <t>qRT‐PCR</t> result of LINC01116 expression in NPC cell lines and the normal HNEpC cells. B, qRT‐PCR result of LINC01116 expression in CNE2 and 5‐8F cells under the transfection of shCtrl or two shRNAs against LINC01116. C, CCK‐8 assay was performed to evaluate cell viability in above cells. D, Cell proliferation was examined by EdU assay. E, Transwell assay for the assessment of cell migration in NPC cells with or without LINC01116 inhibition. * P
    7500 Real Time Polymerase Chain Reaction Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantificational reverse transcription polymerase chain reaction qrt pcr trizol
    Inhibition of LINC01116 suppressed cell proliferation and migration in NPC cells. A, <t>qRT‐PCR</t> result of LINC01116 expression in NPC cell lines and the normal HNEpC cells. B, qRT‐PCR result of LINC01116 expression in CNE2 and 5‐8F cells under the transfection of shCtrl or two shRNAs against LINC01116. C, CCK‐8 assay was performed to evaluate cell viability in above cells. D, Cell proliferation was examined by EdU assay. E, Transwell assay for the assessment of cell migration in NPC cells with or without LINC01116 inhibition. * P
    Quantificational Reverse Transcription Polymerase Chain Reaction Qrt Pcr Trizol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time polymerase chain reaction qrt pcr reagents
    Inhibition of LINC01116 suppressed cell proliferation and migration in NPC cells. A, <t>qRT‐PCR</t> result of LINC01116 expression in NPC cell lines and the normal HNEpC cells. B, qRT‐PCR result of LINC01116 expression in CNE2 and 5‐8F cells under the transfection of shCtrl or two shRNAs against LINC01116. C, CCK‐8 assay was performed to evaluate cell viability in above cells. D, Cell proliferation was examined by EdU assay. E, Transwell assay for the assessment of cell migration in NPC cells with or without LINC01116 inhibition. * P
    Real Time Polymerase Chain Reaction Qrt Pcr Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    HGF/SF mRNA and protein expression in normal colon mucosa and colorectal carcinomas. A: RT-PCR was performed on total RNA isolated from five pairs of normal colon (N) and primary colorectal carcinoma (T), on water (none), and on a plasmid containing full-length human HGF/SF cDNA (pHGF/SF). Primers used were HGF/SF-specific or, as a control, glyceraldehydephosphate dehydrogenase (GAPDH)-specific. The tumors analyzed were CD44- and Met-positive. B: HGF/SF protein expression in colorectal cancer was assessed by immunohistochemistry. a and b : Frozen sections from colorectal cancer tissue were stained with anti-human HGF/SF. This identified cells ( arrows ) in the tumor stroma as HGF/SF-producing cells.

    Journal: The American Journal of Pathology

    Article Title: Expression of c-Met and Heparan-Sulfate Proteoglycan Forms of CD44 in Colorectal Cancer

    doi:

    Figure Lengend Snippet: HGF/SF mRNA and protein expression in normal colon mucosa and colorectal carcinomas. A: RT-PCR was performed on total RNA isolated from five pairs of normal colon (N) and primary colorectal carcinoma (T), on water (none), and on a plasmid containing full-length human HGF/SF cDNA (pHGF/SF). Primers used were HGF/SF-specific or, as a control, glyceraldehydephosphate dehydrogenase (GAPDH)-specific. The tumors analyzed were CD44- and Met-positive. B: HGF/SF protein expression in colorectal cancer was assessed by immunohistochemistry. a and b : Frozen sections from colorectal cancer tissue were stained with anti-human HGF/SF. This identified cells ( arrows ) in the tumor stroma as HGF/SF-producing cells.

    Article Snippet: PCR was performed with 1.5 U Taq DNA Polymerase (Gibco BRL/Life Technologies), 200 μmol/L dNTPs (Pharmacia Biotech, Uppsala, Sweden), and 1.5 mmol/L MgCl2 (2 mmol/L for GAPDH) in 1× PCR Buffer (both Gibco BRL/Life Technologies).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, Plasmid Preparation, Immunohistochemistry, Staining

    Primer Design and qRT-PCR

    Journal: Plastic and reconstructive surgery

    Article Title: Beta-catenin-dependent Wnt signaling: a pathway in acute cutaneous wounding

    doi: 10.1097/PRS.0000000000004170

    Figure Lengend Snippet: Primer Design and qRT-PCR

    Article Snippet: All other reagents for quantitative real-time polymerase chain reaction (qRT-PCR) were purchased from Applied Biosystems (Foster City, CA). rmWnt3a was from R & D Systems (Minneapolis, MN).

    Techniques: Quantitative RT-PCR

    Characterization of apoptosis triggered by TCEA3 expression. a RH30 cells expressing TCEA3 or empty vector control were harvested for RNA. BAX and BCL2 gene expression were assayed by qRT-PCR. Scale bars 50 µm. b RH30 cells expressing TCEA3 or empty vector were assayed by western blot with antibodies against the indicated apoptotic marker proteins. GAPDH was used as a loading control. c RH30 cells expressing TCEA3 and empty vector were treated with caspase 8 inhibitor (Z-IETD-FMK, 40 μM), caspase 9 inhibitor (Z-LEHD-FMK, 40 μM) or a pan-caspase inhibitor (Z-VAD-FMK, 50 μM) for 18 h. Immunofluorescence assay was done with anti TCEA3 antibody (green) and anti-cleaved caspase 3 antibody (red). DAPI (blue) was used to visualize nuclei. Scale bar is 50 µm. d Western blot assay on the same cells as in C. to confirm the protein expression of TCEA3, caspase 3 and tubulin as a loading control. * marks cleaved caspase 3. Error bars are S.E.M. Student t test; *** p

    Journal: Cell Death & Disease

    Article Title: The transcription elongation factor TCEA3 induces apoptosis in rhabdomyosarcoma

    doi: 10.1038/s41419-020-2258-x

    Figure Lengend Snippet: Characterization of apoptosis triggered by TCEA3 expression. a RH30 cells expressing TCEA3 or empty vector control were harvested for RNA. BAX and BCL2 gene expression were assayed by qRT-PCR. Scale bars 50 µm. b RH30 cells expressing TCEA3 or empty vector were assayed by western blot with antibodies against the indicated apoptotic marker proteins. GAPDH was used as a loading control. c RH30 cells expressing TCEA3 and empty vector were treated with caspase 8 inhibitor (Z-IETD-FMK, 40 μM), caspase 9 inhibitor (Z-LEHD-FMK, 40 μM) or a pan-caspase inhibitor (Z-VAD-FMK, 50 μM) for 18 h. Immunofluorescence assay was done with anti TCEA3 antibody (green) and anti-cleaved caspase 3 antibody (red). DAPI (blue) was used to visualize nuclei. Scale bar is 50 µm. d Western blot assay on the same cells as in C. to confirm the protein expression of TCEA3, caspase 3 and tubulin as a loading control. * marks cleaved caspase 3. Error bars are S.E.M. Student t test; *** p

    Article Snippet: Two microgram of total RNA was reverse transcribed with MultiScribe TM MuLV reverse transcriptase (Life Technologies, Carlsbad, CA) and 40 ng of cDNA was used for quantitative real-time polymerase chain reaction (qRT-PCR) amplification (Life Technologies, Carlsbad, CA) with SYBR green PCR master mix (Life Technologies, Carlsbad, CA).

    Techniques: Expressing, Plasmid Preparation, Quantitative RT-PCR, Western Blot, Marker, Immunofluorescence

    TCEA3 expression is downregulated in RMS. a TCEA3 mRNA expression was assayed in C2C12 cells, ARMS (RH28, RH30) and ERMS (RD, RD2) cell lines, respectively, by qRT-PCR. b Western blot with antibodies against TCEA3 to check expression in ERMS (RD, RD2), ARMS (RH28, RH30), and C2C12 cells. c TCEA3 immunostaining with antibodies against TCEA3 and DAPI was used to stain the nuclei. Scale bar is 100 µm. Error bars are S.E.M, Student's t test; *** p

    Journal: Cell Death & Disease

    Article Title: The transcription elongation factor TCEA3 induces apoptosis in rhabdomyosarcoma

    doi: 10.1038/s41419-020-2258-x

    Figure Lengend Snippet: TCEA3 expression is downregulated in RMS. a TCEA3 mRNA expression was assayed in C2C12 cells, ARMS (RH28, RH30) and ERMS (RD, RD2) cell lines, respectively, by qRT-PCR. b Western blot with antibodies against TCEA3 to check expression in ERMS (RD, RD2), ARMS (RH28, RH30), and C2C12 cells. c TCEA3 immunostaining with antibodies against TCEA3 and DAPI was used to stain the nuclei. Scale bar is 100 µm. Error bars are S.E.M, Student's t test; *** p

    Article Snippet: Two microgram of total RNA was reverse transcribed with MultiScribe TM MuLV reverse transcriptase (Life Technologies, Carlsbad, CA) and 40 ng of cDNA was used for quantitative real-time polymerase chain reaction (qRT-PCR) amplification (Life Technologies, Carlsbad, CA) with SYBR green PCR master mix (Life Technologies, Carlsbad, CA).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Immunostaining, Staining

    TCEA3 is repressed by TBX2 and expression correlates with DNA methylation. a RH30 cells depleted for TBX2 or scr control were assayed for mRNA expression of TCEA3 by qRT-PCR. b Chromatin immunoprecipitation (ChIP) assays with antibodies against TBX2 and IgG were assayed with primers against the TCEA3 promoter. c Scatterplot for TCEA3 mRNA expression (RNAseq) and TCEA3 locus methylation level (RRBS) was generated for 834 cancer cell lines using the Cancer Cell Line Encyclopedia (CCLE) database, and the plot was edited using Plotly tool. Each dot represents a cell line. mRNA expression value below 0 have no or negligible expression, and the DNA methylation levels values ranging from 0 to 1 denoting unmethylated to fully methylated locus, respectively. d Inhibition of DNA methylation derepresses TCEA3 expression in RH30 cells. mRNA expression of TCEA3 and TCEA1 were assayed in RH30 cells treated with either DMSO vehicle control or 5-aza-2’deoxycytidine DNA methyltransferase inhibitor at 30 μM and 60 μM concentrations for 48 h by qRT-PCR. Error bars are S.E.M. Student's t test; ns represents ‘not significant’, *** p

    Journal: Cell Death & Disease

    Article Title: The transcription elongation factor TCEA3 induces apoptosis in rhabdomyosarcoma

    doi: 10.1038/s41419-020-2258-x

    Figure Lengend Snippet: TCEA3 is repressed by TBX2 and expression correlates with DNA methylation. a RH30 cells depleted for TBX2 or scr control were assayed for mRNA expression of TCEA3 by qRT-PCR. b Chromatin immunoprecipitation (ChIP) assays with antibodies against TBX2 and IgG were assayed with primers against the TCEA3 promoter. c Scatterplot for TCEA3 mRNA expression (RNAseq) and TCEA3 locus methylation level (RRBS) was generated for 834 cancer cell lines using the Cancer Cell Line Encyclopedia (CCLE) database, and the plot was edited using Plotly tool. Each dot represents a cell line. mRNA expression value below 0 have no or negligible expression, and the DNA methylation levels values ranging from 0 to 1 denoting unmethylated to fully methylated locus, respectively. d Inhibition of DNA methylation derepresses TCEA3 expression in RH30 cells. mRNA expression of TCEA3 and TCEA1 were assayed in RH30 cells treated with either DMSO vehicle control or 5-aza-2’deoxycytidine DNA methyltransferase inhibitor at 30 μM and 60 μM concentrations for 48 h by qRT-PCR. Error bars are S.E.M. Student's t test; ns represents ‘not significant’, *** p

    Article Snippet: Two microgram of total RNA was reverse transcribed with MultiScribe TM MuLV reverse transcriptase (Life Technologies, Carlsbad, CA) and 40 ng of cDNA was used for quantitative real-time polymerase chain reaction (qRT-PCR) amplification (Life Technologies, Carlsbad, CA) with SYBR green PCR master mix (Life Technologies, Carlsbad, CA).

    Techniques: Expressing, DNA Methylation Assay, Quantitative RT-PCR, Chromatin Immunoprecipitation, Methylation, Generated, Inhibition

    Exogenous TCEA3 overexpression in RMS cell lines. a RH30, RH28, RD, and RD2 cell lines were transfected with a TCEA3-expressiion plasmid (pTCEA3) or the empty vector (EV). TCEA3 ectopic expression was confirmed by assaying TCEA3 mRNA expression by qRT-PCR. b Western blot with antibodies against TCEA3 was performed on the cells described in ( a ). to confirm the protein expression of TCEA3. GAPDH was used as the loading control. c TCEA3 immunofluorescence with antibodies against TCEA3 and DAPI used to stain nuclei on cells described in ( a ). Scale bar is 100 µm. Error bars are S.E.M. Student's t test; *** p

    Journal: Cell Death & Disease

    Article Title: The transcription elongation factor TCEA3 induces apoptosis in rhabdomyosarcoma

    doi: 10.1038/s41419-020-2258-x

    Figure Lengend Snippet: Exogenous TCEA3 overexpression in RMS cell lines. a RH30, RH28, RD, and RD2 cell lines were transfected with a TCEA3-expressiion plasmid (pTCEA3) or the empty vector (EV). TCEA3 ectopic expression was confirmed by assaying TCEA3 mRNA expression by qRT-PCR. b Western blot with antibodies against TCEA3 was performed on the cells described in ( a ). to confirm the protein expression of TCEA3. GAPDH was used as the loading control. c TCEA3 immunofluorescence with antibodies against TCEA3 and DAPI used to stain nuclei on cells described in ( a ). Scale bar is 100 µm. Error bars are S.E.M. Student's t test; *** p

    Article Snippet: Two microgram of total RNA was reverse transcribed with MultiScribe TM MuLV reverse transcriptase (Life Technologies, Carlsbad, CA) and 40 ng of cDNA was used for quantitative real-time polymerase chain reaction (qRT-PCR) amplification (Life Technologies, Carlsbad, CA) with SYBR green PCR master mix (Life Technologies, Carlsbad, CA).

    Techniques: Over Expression, Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Staining

    QRT-PCR for 27-hydroxylase and ABCA1 message in NS398-treated THP-1 cells exposed to prostaglandins. (a) 27-Hydroxylase message is decreased by the COX-2 inhibitor NS398 and this decrease is reversed by prostaglandins E 1 , E 2 , and D 2 . THP-1 human monocytes were exposed to the following conditions represented by the six bars (from left to right): (1) RPMI 1640, (2) NS398 (50 μM), (3) PGE 1 (0.1 μM) + NS398 (50 μM), (4) PGE 2 (0.1 μM) + NS398 (50 μM), (5) PGE 1 (0.1 μM) + PGE 2 (0.1 μM) + NS398 (50 μM), and (6) PGD 2 (14 μM) + NS398 (50 μM) (all 18-hour exposures). Cells were extracted for total RNA and were evaluated for 27-hydroxylase mRNA expression by QRT-PCR. Signals obtained from the amplification of GAPDH message were used as internal controls. (b) ABCA1 message is decreased by the COX-2 inhibitor NS398 and this decrease is reversed by prostaglandins E 1 , E 2 , and D 2 . THP-1 human monocytes were exposed to the following conditions represented by the six bars (from left to right): (1) RPMI 1640, (2) NS398 (50 μM), (3) PGE 1 (0.1 μM) + NS398 (50 μM), (4) PGE 2 (0.1 μM) + NS398 (50 μM), (5) PGE 1 (0.1 μM) + PGE 2 (0.1 μM) + NS398 (50 μM), and (6) PGD 2 (14 μM) + NS398 (50 μM) (all 18-hour exposures). Cells were extracted for total RNA and were evaluated for ABCA1 mRNA expression by QRT-PCR. Signals obtained from the amplification of GAPDH message were used as internal controls. ** p

    Journal: Arthritis Research & Therapy

    Article Title: Effect of cyclooxygenase inhibition on cholesterol efflux proteins and atheromatous foam cell transformation in THP-1 human macrophages: a possible mechanism for increased cardiovascular risk

    doi: 10.1186/ar2109

    Figure Lengend Snippet: QRT-PCR for 27-hydroxylase and ABCA1 message in NS398-treated THP-1 cells exposed to prostaglandins. (a) 27-Hydroxylase message is decreased by the COX-2 inhibitor NS398 and this decrease is reversed by prostaglandins E 1 , E 2 , and D 2 . THP-1 human monocytes were exposed to the following conditions represented by the six bars (from left to right): (1) RPMI 1640, (2) NS398 (50 μM), (3) PGE 1 (0.1 μM) + NS398 (50 μM), (4) PGE 2 (0.1 μM) + NS398 (50 μM), (5) PGE 1 (0.1 μM) + PGE 2 (0.1 μM) + NS398 (50 μM), and (6) PGD 2 (14 μM) + NS398 (50 μM) (all 18-hour exposures). Cells were extracted for total RNA and were evaluated for 27-hydroxylase mRNA expression by QRT-PCR. Signals obtained from the amplification of GAPDH message were used as internal controls. (b) ABCA1 message is decreased by the COX-2 inhibitor NS398 and this decrease is reversed by prostaglandins E 1 , E 2 , and D 2 . THP-1 human monocytes were exposed to the following conditions represented by the six bars (from left to right): (1) RPMI 1640, (2) NS398 (50 μM), (3) PGE 1 (0.1 μM) + NS398 (50 μM), (4) PGE 2 (0.1 μM) + NS398 (50 μM), (5) PGE 1 (0.1 μM) + PGE 2 (0.1 μM) + NS398 (50 μM), and (6) PGD 2 (14 μM) + NS398 (50 μM) (all 18-hour exposures). Cells were extracted for total RNA and were evaluated for ABCA1 mRNA expression by QRT-PCR. Signals obtained from the amplification of GAPDH message were used as internal controls. ** p

    Article Snippet: All reagents for reverse transcription and quantitative real-time polymerase chain reaction (QRT-PCR) were purchased from Applied Biosystems (Foster City, CA, USA).

    Techniques: Quantitative RT-PCR, Expressing, Amplification

    QRT-PCR for 27-hydroxylase and ABCA1 message in indomethacin-treated THP-1 cells. (a) 27-Hydroxylase mRNA expression is decreased by the non-specific COX inhibitor indomethacin in a dose-dependent fashion in THP-1 monocytes. Cultured THP-1 monocytic cells were untreated or exposed to increasing doses of indomethacin for 18 hours. After isolation of total RNA, the RNA was reverse-transcribed and the cDNA amplified by QRT-PCR as described. Signals obtained from the amplification of GAPDH message were used as internal controls. (b) ABCA1 mRNA expression is decreased by the non-specific COX inhibitor indomethacin in a dose-dependent fashion in THP-1 monocytes. Cultured THP-1 monocytic cells were untreated or exposed to increasing doses of indomethacin for 18 hours. After isolation of total RNA, the RNA was reverse-transcribed and the cDNA amplified by QRT-PCR as described. Signals obtained from the amplification of GAPDH message were used as internal controls. At 50 mM indomethacin concentration, cell death was statistically significant (16.8% ± 1.0%). ABCA1, ATP-binding cassette transporter A1; COX, cyclooxygenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; QRT-PCR, quantitative real-time polymerase chain reaction. * p

    Journal: Arthritis Research & Therapy

    Article Title: Effect of cyclooxygenase inhibition on cholesterol efflux proteins and atheromatous foam cell transformation in THP-1 human macrophages: a possible mechanism for increased cardiovascular risk

    doi: 10.1186/ar2109

    Figure Lengend Snippet: QRT-PCR for 27-hydroxylase and ABCA1 message in indomethacin-treated THP-1 cells. (a) 27-Hydroxylase mRNA expression is decreased by the non-specific COX inhibitor indomethacin in a dose-dependent fashion in THP-1 monocytes. Cultured THP-1 monocytic cells were untreated or exposed to increasing doses of indomethacin for 18 hours. After isolation of total RNA, the RNA was reverse-transcribed and the cDNA amplified by QRT-PCR as described. Signals obtained from the amplification of GAPDH message were used as internal controls. (b) ABCA1 mRNA expression is decreased by the non-specific COX inhibitor indomethacin in a dose-dependent fashion in THP-1 monocytes. Cultured THP-1 monocytic cells were untreated or exposed to increasing doses of indomethacin for 18 hours. After isolation of total RNA, the RNA was reverse-transcribed and the cDNA amplified by QRT-PCR as described. Signals obtained from the amplification of GAPDH message were used as internal controls. At 50 mM indomethacin concentration, cell death was statistically significant (16.8% ± 1.0%). ABCA1, ATP-binding cassette transporter A1; COX, cyclooxygenase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; QRT-PCR, quantitative real-time polymerase chain reaction. * p

    Article Snippet: All reagents for reverse transcription and quantitative real-time polymerase chain reaction (QRT-PCR) were purchased from Applied Biosystems (Foster City, CA, USA).

    Techniques: Quantitative RT-PCR, Expressing, Cell Culture, Isolation, Amplification, Concentration Assay, Binding Assay, Real-time Polymerase Chain Reaction

    QRT-PCR for 27-hydroxylase and ABCA1 message in NS398-treated THP-1 macrophages exposed to prostaglandins or TXA 2 . (a) 27-Hydroxylase message in THP-1 macrophages is decreased by the COX-2 inhibitor NS398 and this decrease is reversed by prostaglandins E 2 and D 2 , but not TXA 2 . THP-1 human macrophages were exposed to the following conditions represented by the five bars (from left to right): (1) RPMI 1640, (2) NS398 (50 μM), (3) PGE 2 (0.1 μM) + NS398 (50 μM), (4) PGD 2 (14 μM) + NS398 (50 μM), and (5) TXA 2 (3 μM) + NS398 (50 μM) (24-hour exposures to NS398 alone followed by addition of indicated PG or TXA 2 for a further 24 hours). Cells were extracted for total RNA and were evaluated for 27-hydroxylase mRNA expression by QRT-PCR. Signals obtained from the amplification of GAPDH message were used as internal controls. (b) ABCA1 message is decreased by the COX-2 inhibitor NS398 in THP-1 macrophages and this decrease is reversed by prostaglandins E 2 and D 2 , but not TXA 2 . THP-1 human macrophages were exposed to the following conditions represented by the five bars (from left to right): (1) RPMI 1640, (2) NS398 (50 μM), (3) PGE 2 (0.1 μM) + NS398 (50 μM), (4) PGD 2 (14 μM) + NS398 (50 μM), and (5) TXA 2 (3 μM) + NS398 (50 μM) (24-hour exposures to NS398 alone followed by addition of indicated PG or TXA 2 for a further 24 hours). Cells were extracted for total RNA and were evaluated for ABCA1 mRNA expression by QRT-PCR. Signals obtained from the amplification of GAPDH message were used as internal controls. * p

    Journal: Arthritis Research & Therapy

    Article Title: Effect of cyclooxygenase inhibition on cholesterol efflux proteins and atheromatous foam cell transformation in THP-1 human macrophages: a possible mechanism for increased cardiovascular risk

    doi: 10.1186/ar2109

    Figure Lengend Snippet: QRT-PCR for 27-hydroxylase and ABCA1 message in NS398-treated THP-1 macrophages exposed to prostaglandins or TXA 2 . (a) 27-Hydroxylase message in THP-1 macrophages is decreased by the COX-2 inhibitor NS398 and this decrease is reversed by prostaglandins E 2 and D 2 , but not TXA 2 . THP-1 human macrophages were exposed to the following conditions represented by the five bars (from left to right): (1) RPMI 1640, (2) NS398 (50 μM), (3) PGE 2 (0.1 μM) + NS398 (50 μM), (4) PGD 2 (14 μM) + NS398 (50 μM), and (5) TXA 2 (3 μM) + NS398 (50 μM) (24-hour exposures to NS398 alone followed by addition of indicated PG or TXA 2 for a further 24 hours). Cells were extracted for total RNA and were evaluated for 27-hydroxylase mRNA expression by QRT-PCR. Signals obtained from the amplification of GAPDH message were used as internal controls. (b) ABCA1 message is decreased by the COX-2 inhibitor NS398 in THP-1 macrophages and this decrease is reversed by prostaglandins E 2 and D 2 , but not TXA 2 . THP-1 human macrophages were exposed to the following conditions represented by the five bars (from left to right): (1) RPMI 1640, (2) NS398 (50 μM), (3) PGE 2 (0.1 μM) + NS398 (50 μM), (4) PGD 2 (14 μM) + NS398 (50 μM), and (5) TXA 2 (3 μM) + NS398 (50 μM) (24-hour exposures to NS398 alone followed by addition of indicated PG or TXA 2 for a further 24 hours). Cells were extracted for total RNA and were evaluated for ABCA1 mRNA expression by QRT-PCR. Signals obtained from the amplification of GAPDH message were used as internal controls. * p

    Article Snippet: All reagents for reverse transcription and quantitative real-time polymerase chain reaction (QRT-PCR) were purchased from Applied Biosystems (Foster City, CA, USA).

    Techniques: Quantitative RT-PCR, Expressing, Amplification

    ( A ) Hierarchal clustering of differentially expressed microRNAs. Forty-six differentially expressed microRNAs (for up- and down-regulation, respectively) compared to the negative control group in at least one sample were clustered hierarchically. Each rows represent individual microRNAs. The expression ratio representing colour ranges from green (low) to red (high), as indicated by the scale bar. The column bar chart shows the relative expression level of selected microRNAs by qRT-PCR (right column, white) as compared to the fold changes of the corresponding microRNA (left column, grey); ( B ) MicroRNAs expression of Group B versus Group A on the first day of treatment; ( C ) Group B versus Group A on the third day of treatment; ( D ) MicroRNAs expression of on the fifth day of treatment. U87 was used as housekeeping gene. Bars represent the mean ± standard error of three independent experiments; and ( E ) qRT-PCR product was confirmed by gel electrophoresis at 90 V for 40 min.

    Journal: International Journal of Molecular Sciences

    Article Title: MicroRNA Expression Profile of Neural Progenitor-Like Cells Derived from Rat Bone Marrow Mesenchymal Stem Cells under the Influence of IGF-1, bFGF and EGF

    doi: 10.3390/ijms16059693

    Figure Lengend Snippet: ( A ) Hierarchal clustering of differentially expressed microRNAs. Forty-six differentially expressed microRNAs (for up- and down-regulation, respectively) compared to the negative control group in at least one sample were clustered hierarchically. Each rows represent individual microRNAs. The expression ratio representing colour ranges from green (low) to red (high), as indicated by the scale bar. The column bar chart shows the relative expression level of selected microRNAs by qRT-PCR (right column, white) as compared to the fold changes of the corresponding microRNA (left column, grey); ( B ) MicroRNAs expression of Group B versus Group A on the first day of treatment; ( C ) Group B versus Group A on the third day of treatment; ( D ) MicroRNAs expression of on the fifth day of treatment. U87 was used as housekeeping gene. Bars represent the mean ± standard error of three independent experiments; and ( E ) qRT-PCR product was confirmed by gel electrophoresis at 90 V for 40 min.

    Article Snippet: MicroRNA-qPCR Assay MicroRNA expressions were validated using quantitative reverse transcription polymerase chain reaction (qRT-PCR) according to the manufacturer’s protocol (all from Applied Biosystems, Life Technologies Co.; Waltham, MA, USA).

    Techniques: Negative Control, Expressing, Quantitative RT-PCR, Nucleic Acid Electrophoresis

    Inhibition of LINC01116 suppressed cell proliferation and migration in NPC cells. A, qRT‐PCR result of LINC01116 expression in NPC cell lines and the normal HNEpC cells. B, qRT‐PCR result of LINC01116 expression in CNE2 and 5‐8F cells under the transfection of shCtrl or two shRNAs against LINC01116. C, CCK‐8 assay was performed to evaluate cell viability in above cells. D, Cell proliferation was examined by EdU assay. E, Transwell assay for the assessment of cell migration in NPC cells with or without LINC01116 inhibition. * P

    Journal: Cancer Medicine

    Article Title: LINC01116 accelerates nasopharyngeal carcinoma progression based on its enhancement on MYC transcription activity. LINC01116 accelerates nasopharyngeal carcinoma progression based on its enhancement on MYC transcription activity

    doi: 10.1002/cam4.2624

    Figure Lengend Snippet: Inhibition of LINC01116 suppressed cell proliferation and migration in NPC cells. A, qRT‐PCR result of LINC01116 expression in NPC cell lines and the normal HNEpC cells. B, qRT‐PCR result of LINC01116 expression in CNE2 and 5‐8F cells under the transfection of shCtrl or two shRNAs against LINC01116. C, CCK‐8 assay was performed to evaluate cell viability in above cells. D, Cell proliferation was examined by EdU assay. E, Transwell assay for the assessment of cell migration in NPC cells with or without LINC01116 inhibition. * P

    Article Snippet: 2.3 Quantitative real‐time polymerase chain reaction (QRT‐PCR) TRIzol reagent from Invitrogen was utilized to isolate total RNAs from cultured NPC cells.

    Techniques: Inhibition, Migration, Quantitative RT-PCR, Expressing, Transfection, CCK-8 Assay, EdU Assay, Transwell Assay

    MYC activated LINC01116 at transcriptional level in NPC. A‐D, The expression of MYC or LINC01116 in indicated CNE2 and 5‐8F cells was tested by qRT‐PCR. E, ChIP assay and AGE analysis verified the binding of MYC protein to LINC01116 promoter. F,G, Luciferase reporter assay confirmed that LINC01116 was transcriptionally activated by MYC in both CNE2 and 5‐8F cells. * P

    Journal: Cancer Medicine

    Article Title: LINC01116 accelerates nasopharyngeal carcinoma progression based on its enhancement on MYC transcription activity. LINC01116 accelerates nasopharyngeal carcinoma progression based on its enhancement on MYC transcription activity

    doi: 10.1002/cam4.2624

    Figure Lengend Snippet: MYC activated LINC01116 at transcriptional level in NPC. A‐D, The expression of MYC or LINC01116 in indicated CNE2 and 5‐8F cells was tested by qRT‐PCR. E, ChIP assay and AGE analysis verified the binding of MYC protein to LINC01116 promoter. F,G, Luciferase reporter assay confirmed that LINC01116 was transcriptionally activated by MYC in both CNE2 and 5‐8F cells. * P

    Article Snippet: 2.3 Quantitative real‐time polymerase chain reaction (QRT‐PCR) TRIzol reagent from Invitrogen was utilized to isolate total RNAs from cultured NPC cells.

    Techniques: Expressing, Quantitative RT-PCR, Chromatin Immunoprecipitation, Binding Assay, Luciferase, Reporter Assay

    LINC01116 mainly located in the cytoplasm of NPC cells and interacted with the 5ʹUTR of MYC mRNA. A, LINC01116 was predicted by lncLocator as a cytoplasmic lncRNA. B, Subcellular fractionation plus qRT‐PCR validated that LINC01116 was mainly distributed in the cytoplasm of NPC cells. C, The online IntaRNA tool suggested that LINC01116 could bind to the 5'UTR of MYC mRNA. D, The interaction between LINC01116 and MYC mRNA in CNE2 and 5‐8F cells was confirmed by RNA pull down assay. ** P

    Journal: Cancer Medicine

    Article Title: LINC01116 accelerates nasopharyngeal carcinoma progression based on its enhancement on MYC transcription activity. LINC01116 accelerates nasopharyngeal carcinoma progression based on its enhancement on MYC transcription activity

    doi: 10.1002/cam4.2624

    Figure Lengend Snippet: LINC01116 mainly located in the cytoplasm of NPC cells and interacted with the 5ʹUTR of MYC mRNA. A, LINC01116 was predicted by lncLocator as a cytoplasmic lncRNA. B, Subcellular fractionation plus qRT‐PCR validated that LINC01116 was mainly distributed in the cytoplasm of NPC cells. C, The online IntaRNA tool suggested that LINC01116 could bind to the 5'UTR of MYC mRNA. D, The interaction between LINC01116 and MYC mRNA in CNE2 and 5‐8F cells was confirmed by RNA pull down assay. ** P

    Article Snippet: 2.3 Quantitative real‐time polymerase chain reaction (QRT‐PCR) TRIzol reagent from Invitrogen was utilized to isolate total RNAs from cultured NPC cells.

    Techniques: Fractionation, Quantitative RT-PCR, Pull Down Assay

    LINC01116 enhanced MYC protein level so as to promote the transcription of MYC targets. A,B, The impact of LINC01116 on the mRNA and protein levels of MYC was estimated by qRT‐PCR (A) and western blot (B). C, Changes on the transcription of three MYC targets (CDK4, CCND2, and BMI1) in the context of MYC overexpression or together with LINC01116 suppression were evaluated by luciferase reporter assays. D‐E, qRT‐PCR results of the expression of CDK4, CCND2, and BMI1 in LINC01116‐silenced NPC cells. F, The influence of LINC01116 per se on the transcription of CDK4, CCND2, and BMI1 in NPC cells was determined by luciferase reporter assay. * P

    Journal: Cancer Medicine

    Article Title: LINC01116 accelerates nasopharyngeal carcinoma progression based on its enhancement on MYC transcription activity. LINC01116 accelerates nasopharyngeal carcinoma progression based on its enhancement on MYC transcription activity

    doi: 10.1002/cam4.2624

    Figure Lengend Snippet: LINC01116 enhanced MYC protein level so as to promote the transcription of MYC targets. A,B, The impact of LINC01116 on the mRNA and protein levels of MYC was estimated by qRT‐PCR (A) and western blot (B). C, Changes on the transcription of three MYC targets (CDK4, CCND2, and BMI1) in the context of MYC overexpression or together with LINC01116 suppression were evaluated by luciferase reporter assays. D‐E, qRT‐PCR results of the expression of CDK4, CCND2, and BMI1 in LINC01116‐silenced NPC cells. F, The influence of LINC01116 per se on the transcription of CDK4, CCND2, and BMI1 in NPC cells was determined by luciferase reporter assay. * P

    Article Snippet: 2.3 Quantitative real‐time polymerase chain reaction (QRT‐PCR) TRIzol reagent from Invitrogen was utilized to isolate total RNAs from cultured NPC cells.

    Techniques: Quantitative RT-PCR, Western Blot, Over Expression, Luciferase, Expressing, Reporter Assay

    Overexpression of MYC rescued LINC01116 depletion‐restrained proliferation and migration in NPC cells. A, The expression of MYC at both mRNA and protein levels in indicated cells was detected via qRT‐PCR and western blot. B‐D, The viability, proliferation, and migration in 5‐8F cells under different conditions were respectively evaluated through CCK‐8 (B), EdU (C), and transwell assays (D). * P

    Journal: Cancer Medicine

    Article Title: LINC01116 accelerates nasopharyngeal carcinoma progression based on its enhancement on MYC transcription activity. LINC01116 accelerates nasopharyngeal carcinoma progression based on its enhancement on MYC transcription activity

    doi: 10.1002/cam4.2624

    Figure Lengend Snippet: Overexpression of MYC rescued LINC01116 depletion‐restrained proliferation and migration in NPC cells. A, The expression of MYC at both mRNA and protein levels in indicated cells was detected via qRT‐PCR and western blot. B‐D, The viability, proliferation, and migration in 5‐8F cells under different conditions were respectively evaluated through CCK‐8 (B), EdU (C), and transwell assays (D). * P

    Article Snippet: 2.3 Quantitative real‐time polymerase chain reaction (QRT‐PCR) TRIzol reagent from Invitrogen was utilized to isolate total RNAs from cultured NPC cells.

    Techniques: Over Expression, Migration, Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay