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  • 99
    Thermo Fisher 7500 fast real time pcr system
    7500 Fast Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31629 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa real time polymerase chain reaction qrt pcr
    Knockdown of FOXQ1 partially inhibited TGF-β1-induced aggressive tumor behaviors in SW480 cells and the changes in expression of downstream genes. ( A ) NC- and FOXQ1-shRNA cells were incubated in growth media supplemented with TGF-β1 (3 ng/ml) for 3 days. FOXQ1 expression was measured by <t>qRT-PCR</t> and Western blot. ( B ) The culture supernatant from NC-shRNA cells cultured for 3 days or NC- and FOXQ1-shRNA cells cultured for 5 days containing TGF-β1 (3 ng/ml) was collected and used in an in vitro angiogenesis assay. The vessel formation was measured in NC-shRNA-transduced EA.hy926 cells. The number of vessels was counted by fluorescent microscopy (120X). ( C ) FOXQ1- and NC-shRNA cells were treated with TGF-β1 (3 ng/ml) for 5 days, and cell morphology was examined by optical microscopy (120X) (upper panel). For the invasion assay, FOXQ1- and NC-shRNA cells were pre-treated with TGF-β1 (3 ng/ml) for 3 days before being seeded in the upper chamber of Transwell. After 36 hours, the cells migrating through the insert membrane were fixed and stained by crystal violet. The number of migrated cells was counted by optical microscopy (120X) (lower panels). ( D ) For the drug sensitivity assay, FOXQ1- and NC-shRNA cells were incubated in TGF-β1-containing media (3 ng/ml) for 4 days, and 5-FU or L-OHP (100 μg/ml) was added to the cell culture for an additional 24 hours. Flow cytometry was performed to measure cell apoptosis. ( E ) Expression of Wnt downstream target genes and EMT-associated markers in TGF-β1 treated NC- and FOXQ1-shRNA cells were measured by qRT-PCR and Western blot. The results are expressed as mean ± SD *P
    Real Time Polymerase Chain Reaction Qrt Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative real time polymerase chain reaction qrt pcr kits
    Detection of the expression of miR-218 and miR-34a in tissue specimens by <t>qRT-PCR.</t> Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P
    Quantitative Real Time Polymerase Chain Reaction Qrt Pcr Kits, supplied by TaKaRa, used in various techniques. Bioz Stars score: 82/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa real time polymerase chain reaction qrt pcr reagents
    Detection of the expression of miR-218 and miR-34a in tissue specimens by <t>qRT-PCR.</t> Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P
    Real Time Polymerase Chain Reaction Qrt Pcr Reagents, supplied by TaKaRa, used in various techniques. Bioz Stars score: 82/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative real time polymerase chain reaction qrt pcr rnaiso plus reagent
    Detection of the expression of miR-218 and miR-34a in tissue specimens by <t>qRT-PCR.</t> Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P
    Quantitative Real Time Polymerase Chain Reaction Qrt Pcr Rnaiso Plus Reagent, supplied by TaKaRa, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa lenti xtm quantitative reverse transcriptase polymerase chain reaction qrt pcr titration kit
    Detection of the expression of miR-218 and miR-34a in tissue specimens by <t>qRT-PCR.</t> Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P
    Lenti Xtm Quantitative Reverse Transcriptase Polymerase Chain Reaction Qrt Pcr Titration Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr green real time quantitative polymerase chain reaction qrt pcr analysis
    Detection of the expression of miR-218 and miR-34a in tissue specimens by <t>qRT-PCR.</t> Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P
    Sybr Green Real Time Quantitative Polymerase Chain Reaction Qrt Pcr Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative real time polymerase chain reaction qrt pcr sybr green pcr master mix
    Detection of the expression of miR-218 and miR-34a in tissue specimens by <t>qRT-PCR.</t> Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P
    Quantitative Real Time Polymerase Chain Reaction Qrt Pcr Sybr Green Pcr Master Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative real time polymerase chain reactions qrt pcr one step sybr primescript rt pcr kit
    Detection of the expression of miR-218 and miR-34a in tissue specimens by <t>qRT-PCR.</t> Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P
    Quantitative Real Time Polymerase Chain Reactions Qrt Pcr One Step Sybr Primescript Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa real time polymerase chain reaction
    Detection of the expression of miR-218 and miR-34a in tissue specimens by <t>qRT-PCR.</t> Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P
    Real Time Polymerase Chain Reaction, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa qrt pcr analysis
    Detection of the expression of miR-218 and miR-34a in tissue specimens by <t>qRT-PCR.</t> Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P
    Qrt Pcr Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1703 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa qrt pcr kit
    Expression of TaCAMTA4 in different combination plants after P . triticina infection. The transcription levels of wheat TaCAMTA4 were detected by <t>qRT-PCR</t> in both incompatible and compatible combinations at different time points after P . triticina infection. Mock treatment combination was used as control. Constitutively expressed gene EF-1-α was used as a reference. Results are means ± SD with three replicates.
    Qrt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr qrt pcr
    Expression of TaCAMTA4 in different combination plants after P . triticina infection. The transcription levels of wheat TaCAMTA4 were detected by <t>qRT-PCR</t> in both incompatible and compatible combinations at different time points after P . triticina infection. Mock treatment combination was used as control. Constitutively expressed gene EF-1-α was used as a reference. Results are means ± SD with three replicates.
    Sybr Qrt Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa qrt pcr assays
    Relative miR-186 and Twist1 expression in GC tissues a., b. miR-186 and Twist1 expression levels in GC and normal tissues were analyzed using <t>qRT-PCR.</t> miR-186 and Twist1 expression were examined by qRT-PCR, and normalized to U6 and GAPDH expression (shown as ΔCT) *p
    Qrt Pcr Assays, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr qrt pcr kit
    <t>QRT-PCR</t> validation of lipase , RNA helicase , PP2C , H + -ATPase , WRKY , and 14-3-3 genes. Panels A and B show gene expressions in blue light and dark, respectively. Expression changes are presented as normalized fold changes between the test tissues and reference tissue (WT/MS) in corresponding B and D. Positive and negative values indicate up and down regulations of the gene expression, respectively. Twofold threshold was considered as a cutoff value for significant changes in the expression. Error bars represent standard errors of three technical replicates based on DMNRT (p = 0.05).
    Sybr Qrt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 167 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa qrt pcr trizol reagent
    <t>QRT-PCR</t> validation of lipase , RNA helicase , PP2C , H + -ATPase , WRKY , and 14-3-3 genes. Panels A and B show gene expressions in blue light and dark, respectively. Expression changes are presented as normalized fold changes between the test tissues and reference tissue (WT/MS) in corresponding B and D. Positive and negative values indicate up and down regulations of the gene expression, respectively. Twofold threshold was considered as a cutoff value for significant changes in the expression. Error bars represent standard errors of three technical replicates based on DMNRT (p = 0.05).
    Qrt Pcr Trizol Reagent, supplied by TaKaRa, used in various techniques. Bioz Stars score: 83/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr green qrt pcr
    Sialyltransferase ST 3 GAL 6 was upregulated in human hepatocellular carcinoma ( HCC ) tissues and cell lines. (a) Differences in expression in HCC cell lines ( MHCC 97‐H, MHCC 97‐L, and HCCLM 3) and a normal human hepatic cell line ( LO 2). (b) ST 3 GAL 6 (measured by <t>SYBR</t> Green quantitative real‐time <t>PCR</t> ) was upregulated in HCC tissues compared with the matched adjacent non‐tumorous liver tissues. The central horizontal line represents the mean value. * P
    Sybr Green Qrt Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr qrt pcr analysis
    Sialyltransferase ST 3 GAL 6 was upregulated in human hepatocellular carcinoma ( HCC ) tissues and cell lines. (a) Differences in expression in HCC cell lines ( MHCC 97‐H, MHCC 97‐L, and HCCLM 3) and a normal human hepatic cell line ( LO 2). (b) ST 3 GAL 6 (measured by <t>SYBR</t> Green quantitative real‐time <t>PCR</t> ) was upregulated in HCC tissues compared with the matched adjacent non‐tumorous liver tissues. The central horizontal line represents the mean value. * P
    Sybr Qrt Pcr Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa a sybr green pcr kit
    Sialyltransferase ST 3 GAL 6 was upregulated in human hepatocellular carcinoma ( HCC ) tissues and cell lines. (a) Differences in expression in HCC cell lines ( MHCC 97‐H, MHCC 97‐L, and HCCLM 3) and a normal human hepatic cell line ( LO 2). (b) ST 3 GAL 6 (measured by <t>SYBR</t> Green quantitative real‐time <t>PCR</t> ) was upregulated in HCC tissues compared with the matched adjacent non‐tumorous liver tissues. The central horizontal line represents the mean value. * P
    A Sybr Green Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr green qrt pcr kit
    Sertoli cell markers are dysregulated in Pin1 −/− testes. ( A ) Amh (antimüllerian hormone) mRNA transcript expression was analyzed in Pin1 +/+ and Pin1 −/− testes. ( B ) Ar (androgen receptor) and ( C ) Gja1/connexin 43 mRNA levels were also analyzed in the same tissue. ( D ) The expression profile of the Sertoli cell-specific marker Wt1 (Wilms tumor) was analyzed by <t>qRT-PCR.</t> RNA from whole snap-frozen testicular tissue was extracted by Trizol digestion after homogenization using a mortar. qRT-PCR was performed using <t>Sybr</t> green reagent in an Applied Biosystems 2500 thermocycler.
    Sybr Green Qrt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa revertra ace qrt pcr kit
    Correlations between CLIC1 and PA28B , MMP2 , MMP9 , ANXA7 , GSN , MMP12 , MMP13 , and SERPINB5 mRNA in OSCC according to <t>qRT-PCR</t> data. Notes: ( A ) Correlation between CLIC1 and PA28B . ( B ) Correlation between CLIC1 and MMP2 . ( C ) Correlation between CLIC1 and MMP9 . ( D ) Correlation between CLIC1 and ANXA7 . ( E ) Correlation between CLIC1 and GSN . ( F ) Correlation between CLIC1 and MMP12 . ( G ) Correlation between CLIC1 and MMP13 . ( H ) Correlation between CLIC1 and SERPINB5 . r -values calculated by Pearson correlation analysis. P -values calculated by Student’s t -test. Abbreviations: OSCC, oral squamous cell carcinoma; qRT-PCR, quantitative real-time PCR.
    Revertra Ace Qrt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 84/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa prime script qrt pcr kit
    Suppress activity of B.subtilis or surfactin. Cells were exposed to B. subtilis 168, OKB105 and surfactin in different treatments as described above. For the indicated time points, cells were collected and the yield of virus was determined by <t>qRT-PCR</t> ( A ) and Western blotting ( B ). (B) Lane 1, TGEV control; lane 2, virus from cells treated with 0.002 mg/ml surfactin; lane 3, virus from cells treated with 1.00E + 07 cfu/ml B. subtilis 168; lane 4, virus from cells treated with 1.00E + 07 cfu/ml B. subtilis OKB105; lane 5, mock. ( C ) Mean relative protein ratio of TGEV-N. Blots were reported with antibody to GAPDH as a loading control. The mean ± S.D. from three independent experiments are shown. Significance levels for the differences between B. subtilis and surfactin treatments and virus control from untreated cells are given above the bar: * P
    Prime Script Qrt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa qrt pcr rnaiso plus reagent
    High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) <t>qRT-PCR</t> assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p
    Qrt Pcr Rnaiso Plus Reagent, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa lenti x qrt pcr kit
    High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) <t>qRT-PCR</t> assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p
    Lenti X Qrt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa qrt pcr analysis trizol reagent
    High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) <t>qRT-PCR</t> assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p
    Qrt Pcr Analysis Trizol Reagent, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa mirna specific qrt pcr
    High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) <t>qRT-PCR</t> assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p
    Mirna Specific Qrt Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr green qrt pcr assay
    High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) <t>qRT-PCR</t> assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p
    Sybr Green Qrt Pcr Assay, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr green qrt pcr analysis
    High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) <t>qRT-PCR</t> assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p
    Sybr Green Qrt Pcr Analysis, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) <t>qRT-PCR</t> assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p
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    High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) <t>qRT-PCR</t> assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p
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    High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) <t>qRT-PCR</t> assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p
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    Image Search Results


    Knockdown of FOXQ1 partially inhibited TGF-β1-induced aggressive tumor behaviors in SW480 cells and the changes in expression of downstream genes. ( A ) NC- and FOXQ1-shRNA cells were incubated in growth media supplemented with TGF-β1 (3 ng/ml) for 3 days. FOXQ1 expression was measured by qRT-PCR and Western blot. ( B ) The culture supernatant from NC-shRNA cells cultured for 3 days or NC- and FOXQ1-shRNA cells cultured for 5 days containing TGF-β1 (3 ng/ml) was collected and used in an in vitro angiogenesis assay. The vessel formation was measured in NC-shRNA-transduced EA.hy926 cells. The number of vessels was counted by fluorescent microscopy (120X). ( C ) FOXQ1- and NC-shRNA cells were treated with TGF-β1 (3 ng/ml) for 5 days, and cell morphology was examined by optical microscopy (120X) (upper panel). For the invasion assay, FOXQ1- and NC-shRNA cells were pre-treated with TGF-β1 (3 ng/ml) for 3 days before being seeded in the upper chamber of Transwell. After 36 hours, the cells migrating through the insert membrane were fixed and stained by crystal violet. The number of migrated cells was counted by optical microscopy (120X) (lower panels). ( D ) For the drug sensitivity assay, FOXQ1- and NC-shRNA cells were incubated in TGF-β1-containing media (3 ng/ml) for 4 days, and 5-FU or L-OHP (100 μg/ml) was added to the cell culture for an additional 24 hours. Flow cytometry was performed to measure cell apoptosis. ( E ) Expression of Wnt downstream target genes and EMT-associated markers in TGF-β1 treated NC- and FOXQ1-shRNA cells were measured by qRT-PCR and Western blot. The results are expressed as mean ± SD *P

    Journal: Cancer Biology & Therapy

    Article Title: FOXQ1 mediates the crosstalk between TGF-β and Wnt signaling pathways in the progression of colorectal cancer

    doi: 10.1080/15384047.2015.1047568

    Figure Lengend Snippet: Knockdown of FOXQ1 partially inhibited TGF-β1-induced aggressive tumor behaviors in SW480 cells and the changes in expression of downstream genes. ( A ) NC- and FOXQ1-shRNA cells were incubated in growth media supplemented with TGF-β1 (3 ng/ml) for 3 days. FOXQ1 expression was measured by qRT-PCR and Western blot. ( B ) The culture supernatant from NC-shRNA cells cultured for 3 days or NC- and FOXQ1-shRNA cells cultured for 5 days containing TGF-β1 (3 ng/ml) was collected and used in an in vitro angiogenesis assay. The vessel formation was measured in NC-shRNA-transduced EA.hy926 cells. The number of vessels was counted by fluorescent microscopy (120X). ( C ) FOXQ1- and NC-shRNA cells were treated with TGF-β1 (3 ng/ml) for 5 days, and cell morphology was examined by optical microscopy (120X) (upper panel). For the invasion assay, FOXQ1- and NC-shRNA cells were pre-treated with TGF-β1 (3 ng/ml) for 3 days before being seeded in the upper chamber of Transwell. After 36 hours, the cells migrating through the insert membrane were fixed and stained by crystal violet. The number of migrated cells was counted by optical microscopy (120X) (lower panels). ( D ) For the drug sensitivity assay, FOXQ1- and NC-shRNA cells were incubated in TGF-β1-containing media (3 ng/ml) for 4 days, and 5-FU or L-OHP (100 μg/ml) was added to the cell culture for an additional 24 hours. Flow cytometry was performed to measure cell apoptosis. ( E ) Expression of Wnt downstream target genes and EMT-associated markers in TGF-β1 treated NC- and FOXQ1-shRNA cells were measured by qRT-PCR and Western blot. The results are expressed as mean ± SD *P

    Article Snippet: All reagents and primers used in quantitative real-time polymerase chain reaction (qRT-PCR) were purchased from Takara Bio (Dalian, China).

    Techniques: Expressing, shRNA, Incubation, Quantitative RT-PCR, Western Blot, Cell Culture, In Vitro, Angiogenesis Assay, Microscopy, Invasion Assay, Staining, Sensitive Assay, Flow Cytometry, Cytometry

    Detection of the expression of miR-218 and miR-34a in tissue specimens by qRT-PCR. Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P

    Journal: Oncology Letters

    Article Title: Values of miR-34a and miR-218 expression in the diagnosis of cervical cancer and the prediction of prognosis

    doi: 10.3892/ol.2018.7791

    Figure Lengend Snippet: Detection of the expression of miR-218 and miR-34a in tissue specimens by qRT-PCR. Compared with those in normal cervical tissues, the expression levels of miR-34a and miR-218 in cervical cancer tissues are significantly decreased, **P

    Article Snippet: The present study was approved by the Clinical Ethics Committee, and all the patients or family members signed the informed consent; TRIzol kit (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA); primer synthesis, reverse transcription kits and quantitative real-time polymerase chain reaction (qRT-PCR) kits (all from Takara Biotechnology Co., Ltd., Dalian, China).

    Techniques: Expressing, Quantitative RT-PCR

    Expression of TaCAMTA4 in different combination plants after P . triticina infection. The transcription levels of wheat TaCAMTA4 were detected by qRT-PCR in both incompatible and compatible combinations at different time points after P . triticina infection. Mock treatment combination was used as control. Constitutively expressed gene EF-1-α was used as a reference. Results are means ± SD with three replicates.

    Journal: Scientific Reports

    Article Title: TaCAMTA4, a Calmodulin-Interacting Protein, Involved in Defense Response of Wheat to Puccinia triticina

    doi: 10.1038/s41598-018-36385-1

    Figure Lengend Snippet: Expression of TaCAMTA4 in different combination plants after P . triticina infection. The transcription levels of wheat TaCAMTA4 were detected by qRT-PCR in both incompatible and compatible combinations at different time points after P . triticina infection. Mock treatment combination was used as control. Constitutively expressed gene EF-1-α was used as a reference. Results are means ± SD with three replicates.

    Article Snippet: RNA was purified and reverse transcribed to synthesis the first strand of cDNA. qRT-PCR was conducted using qRT-PCR kit (TaKaRa).

    Techniques: Expressing, Infection, Quantitative RT-PCR

    TaCAMTA4 negatively regulate the defense response of wheat to P . triticina . ( a ) Expression analysis of TaCAMTA4 in the third leaves inoculated with BSMV: 00 and BSMV: TaCAMTA4 by qRT-PCR. After 12 days BSMV inoculation, the mature urediniospores of P . triticina race 165 were brushed on the newly emerged third leaves. Then the RNA extracted from the third leaves at 48 h and 72 h after P . triticina infection was subjected to qRT-PCR. ( b ) Biology microscope observations of P . triticina mycelia appearing on leaf pieces at 48 h and 72 h after inoculation. Leaf tissue was stained with 0.1% Florescent Brightener (FB) Bar = 100 μm. ( c ) The average area of mycelia was statistically analyzed between BSMV: 00(control) and BSMV: TaCAMTA 4. Values are means (±SD) of 30 mycelia. The numbers of mycelia per visual field were counted at 48 h and 72 h after P . triticina infection. ( d ) The phenotype of uredosorus after inoculation with P . triticina . Photos were taken at 13 days after P . triticina infection.

    Journal: Scientific Reports

    Article Title: TaCAMTA4, a Calmodulin-Interacting Protein, Involved in Defense Response of Wheat to Puccinia triticina

    doi: 10.1038/s41598-018-36385-1

    Figure Lengend Snippet: TaCAMTA4 negatively regulate the defense response of wheat to P . triticina . ( a ) Expression analysis of TaCAMTA4 in the third leaves inoculated with BSMV: 00 and BSMV: TaCAMTA4 by qRT-PCR. After 12 days BSMV inoculation, the mature urediniospores of P . triticina race 165 were brushed on the newly emerged third leaves. Then the RNA extracted from the third leaves at 48 h and 72 h after P . triticina infection was subjected to qRT-PCR. ( b ) Biology microscope observations of P . triticina mycelia appearing on leaf pieces at 48 h and 72 h after inoculation. Leaf tissue was stained with 0.1% Florescent Brightener (FB) Bar = 100 μm. ( c ) The average area of mycelia was statistically analyzed between BSMV: 00(control) and BSMV: TaCAMTA 4. Values are means (±SD) of 30 mycelia. The numbers of mycelia per visual field were counted at 48 h and 72 h after P . triticina infection. ( d ) The phenotype of uredosorus after inoculation with P . triticina . Photos were taken at 13 days after P . triticina infection.

    Article Snippet: RNA was purified and reverse transcribed to synthesis the first strand of cDNA. qRT-PCR was conducted using qRT-PCR kit (TaKaRa).

    Techniques: Expressing, Quantitative RT-PCR, Infection, Microscopy, Staining

    Relative miR-186 and Twist1 expression in GC tissues a., b. miR-186 and Twist1 expression levels in GC and normal tissues were analyzed using qRT-PCR. miR-186 and Twist1 expression were examined by qRT-PCR, and normalized to U6 and GAPDH expression (shown as ΔCT) *p

    Journal: Oncotarget

    Article Title: miR-186 affects the proliferation, invasion and migration of human gastric cancer by inhibition of Twist1

    doi: 10.18632/oncotarget.13182

    Figure Lengend Snippet: Relative miR-186 and Twist1 expression in GC tissues a., b. miR-186 and Twist1 expression levels in GC and normal tissues were analyzed using qRT-PCR. miR-186 and Twist1 expression were examined by qRT-PCR, and normalized to U6 and GAPDH expression (shown as ΔCT) *p

    Article Snippet: 1 μg total RNA was reverse transcribed to cDNA by using a reverse transcription kit for next qRT-PCR assays (Takara, Dalian, China).

    Techniques: Expressing, Quantitative RT-PCR

    AplD upregulation upon K. pneumoniae feeding and during late development. (A) AplD transcription profile in xenic Ax2 cultures were examined by mixing amoebae with bacteria at an 1:10 ratio and measuring aplD transcript levels by qRT-PCR. AplD fold expression in xenic Ax2 cultures were determined based on aplD transcription in axenic Ax2 cultures. Bacterial strains tested were K. pneumoniae ( Kp LM21), B. subtilis ( Bs ), and P. aeruginosa ( Pa ). Each symbol denotes the data from an individual experiment and the bar represents the median thereof. (B) AplD regulation during Ax2 development was tested by subjecting Ax2 for development on KK2 agar plates and quantifying aplD transcription at different morphogenetic stages by qRT-PCR. AplD expression at 0 h was considered as reference to determine aplD fold expression at subsequent development stages. n = 3, bars represent standard deviation.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The Saposin-Like Protein AplD Displays Pore-Forming Activity and Participates in Defense Against Bacterial Infection During a Multicellular Stage of Dictyostelium discoideum

    doi: 10.3389/fcimb.2018.00073

    Figure Lengend Snippet: AplD upregulation upon K. pneumoniae feeding and during late development. (A) AplD transcription profile in xenic Ax2 cultures were examined by mixing amoebae with bacteria at an 1:10 ratio and measuring aplD transcript levels by qRT-PCR. AplD fold expression in xenic Ax2 cultures were determined based on aplD transcription in axenic Ax2 cultures. Bacterial strains tested were K. pneumoniae ( Kp LM21), B. subtilis ( Bs ), and P. aeruginosa ( Pa ). Each symbol denotes the data from an individual experiment and the bar represents the median thereof. (B) AplD regulation during Ax2 development was tested by subjecting Ax2 for development on KK2 agar plates and quantifying aplD transcription at different morphogenetic stages by qRT-PCR. AplD expression at 0 h was considered as reference to determine aplD fold expression at subsequent development stages. n = 3, bars represent standard deviation.

    Article Snippet: Quantitative RT-PCR (qRT-PCR) was performed using the cDNA template as described by the manufacturer (qRT-PCR mix, TAKARA and Light cycler, Roche).

    Techniques: Quantitative RT-PCR, Expressing, Standard Deviation

    QRT-PCR validation of lipase , RNA helicase , PP2C , H + -ATPase , WRKY , and 14-3-3 genes. Panels A and B show gene expressions in blue light and dark, respectively. Expression changes are presented as normalized fold changes between the test tissues and reference tissue (WT/MS) in corresponding B and D. Positive and negative values indicate up and down regulations of the gene expression, respectively. Twofold threshold was considered as a cutoff value for significant changes in the expression. Error bars represent standard errors of three technical replicates based on DMNRT (p = 0.05).

    Journal: PLoS ONE

    Article Title: DNA Methylation and Transcriptomic Changes in Response to Different Lights and Stresses in 7B-1 Male-Sterile Tomato

    doi: 10.1371/journal.pone.0121864

    Figure Lengend Snippet: QRT-PCR validation of lipase , RNA helicase , PP2C , H + -ATPase , WRKY , and 14-3-3 genes. Panels A and B show gene expressions in blue light and dark, respectively. Expression changes are presented as normalized fold changes between the test tissues and reference tissue (WT/MS) in corresponding B and D. Positive and negative values indicate up and down regulations of the gene expression, respectively. Twofold threshold was considered as a cutoff value for significant changes in the expression. Error bars represent standard errors of three technical replicates based on DMNRT (p = 0.05).

    Article Snippet: PCR conditions were set at 95°C for 2 min, followed by 40 cycles of 95°C for 5 s, and annealing/extension at 60°C for 20 s. Expressions of miRNAs and tasiRNAs were validated using the Mir-X miRNA First-Strand Synthesis and SYBR qRT-PCR kit (Clontech).

    Techniques: Quantitative RT-PCR, Expressing, Mass Spectrometry

    QRT-PCR validation of CRY1 , CRY2 , PHOT1 , PHOT2 , and HY5 genes in 7B-1 and WT seedlings in response to 5-azaC. Expression changes are presented as normalized fold changes between the test tissues and reference tissue (untreated WT). Positive and negative values indicate up and down regulations of the gene expression, respectively. Twofold threshold was considered as a cutoff value for significant changes in the expression. Error bars represent standard errors of three technical replicates based on DMNRT (p = 0.05).

    Journal: PLoS ONE

    Article Title: DNA Methylation and Transcriptomic Changes in Response to Different Lights and Stresses in 7B-1 Male-Sterile Tomato

    doi: 10.1371/journal.pone.0121864

    Figure Lengend Snippet: QRT-PCR validation of CRY1 , CRY2 , PHOT1 , PHOT2 , and HY5 genes in 7B-1 and WT seedlings in response to 5-azaC. Expression changes are presented as normalized fold changes between the test tissues and reference tissue (untreated WT). Positive and negative values indicate up and down regulations of the gene expression, respectively. Twofold threshold was considered as a cutoff value for significant changes in the expression. Error bars represent standard errors of three technical replicates based on DMNRT (p = 0.05).

    Article Snippet: PCR conditions were set at 95°C for 2 min, followed by 40 cycles of 95°C for 5 s, and annealing/extension at 60°C for 20 s. Expressions of miRNAs and tasiRNAs were validated using the Mir-X miRNA First-Strand Synthesis and SYBR qRT-PCR kit (Clontech).

    Techniques: Quantitative RT-PCR, Expressing

    QRT-PCR validation of miRNAs, tasiRNAs, and ARF s in 5-azaC-treated 7B-1 seedlings. Expression changes are presented as normalized fold changes between the test tissues and reference tissue (untreated 7B-1 ). Positive and negative values indicate up and down regulations of the gene expression, respectively. Twofold threshold was considered as a cutoff value for significant changes in the expression. Error bars represent standard errors of three technical replicates based on DMNRT (p = 0.05).

    Journal: PLoS ONE

    Article Title: DNA Methylation and Transcriptomic Changes in Response to Different Lights and Stresses in 7B-1 Male-Sterile Tomato

    doi: 10.1371/journal.pone.0121864

    Figure Lengend Snippet: QRT-PCR validation of miRNAs, tasiRNAs, and ARF s in 5-azaC-treated 7B-1 seedlings. Expression changes are presented as normalized fold changes between the test tissues and reference tissue (untreated 7B-1 ). Positive and negative values indicate up and down regulations of the gene expression, respectively. Twofold threshold was considered as a cutoff value for significant changes in the expression. Error bars represent standard errors of three technical replicates based on DMNRT (p = 0.05).

    Article Snippet: PCR conditions were set at 95°C for 2 min, followed by 40 cycles of 95°C for 5 s, and annealing/extension at 60°C for 20 s. Expressions of miRNAs and tasiRNAs were validated using the Mir-X miRNA First-Strand Synthesis and SYBR qRT-PCR kit (Clontech).

    Techniques: Quantitative RT-PCR, Expressing

    QRT-PCR validation of the differentially regulated genes in response to 5-azaC treatment. Expression changes are presented as normalized fold changes between the test tissues and reference tissue (untreated WT). Positive and negative values indicate up and down regulations of the gene expression, respectively. Twofold threshold was considered as a cutoff value for significant changes in the expression. Error bars represent standard errors of three technical replicates based on DMNRT (p = 0.05).

    Journal: PLoS ONE

    Article Title: DNA Methylation and Transcriptomic Changes in Response to Different Lights and Stresses in 7B-1 Male-Sterile Tomato

    doi: 10.1371/journal.pone.0121864

    Figure Lengend Snippet: QRT-PCR validation of the differentially regulated genes in response to 5-azaC treatment. Expression changes are presented as normalized fold changes between the test tissues and reference tissue (untreated WT). Positive and negative values indicate up and down regulations of the gene expression, respectively. Twofold threshold was considered as a cutoff value for significant changes in the expression. Error bars represent standard errors of three technical replicates based on DMNRT (p = 0.05).

    Article Snippet: PCR conditions were set at 95°C for 2 min, followed by 40 cycles of 95°C for 5 s, and annealing/extension at 60°C for 20 s. Expressions of miRNAs and tasiRNAs were validated using the Mir-X miRNA First-Strand Synthesis and SYBR qRT-PCR kit (Clontech).

    Techniques: Quantitative RT-PCR, Expressing

    qRT-PCR analysis of pfa1 expression in relation to growth phase (A) and growth conditions (B and C). (A) qRT-PCR was performed with total RNA extracted from P. fluorescens strain TSS cultured to different densities in LB medium. (B and C) qRT-PCR analyses

    Journal:

    Article Title: Identification, Characterization, and Molecular Application of a Virulence-Associated Autotransporter from a Pathogenic Pseudomonas fluorescens Strain ▿ Strain ▿ †

    doi: 10.1128/AEM.00159-09

    Figure Lengend Snippet: qRT-PCR analysis of pfa1 expression in relation to growth phase (A) and growth conditions (B and C). (A) qRT-PCR was performed with total RNA extracted from P. fluorescens strain TSS cultured to different densities in LB medium. (B and C) qRT-PCR analyses

    Article Snippet: Quantitative real-time reverse transcriptase PCR (qRT-PCR) was carried out in an ABI 7300 real-time detection system (Applied Biosystems) by using a SYBR ExScript qRT-PCR kit (Takara) as described previously ( ).

    Techniques: Quantitative RT-PCR, Expressing, Cell Culture

    Sialyltransferase ST 3 GAL 6 was upregulated in human hepatocellular carcinoma ( HCC ) tissues and cell lines. (a) Differences in expression in HCC cell lines ( MHCC 97‐H, MHCC 97‐L, and HCCLM 3) and a normal human hepatic cell line ( LO 2). (b) ST 3 GAL 6 (measured by SYBR Green quantitative real‐time PCR ) was upregulated in HCC tissues compared with the matched adjacent non‐tumorous liver tissues. The central horizontal line represents the mean value. * P

    Journal: Cancer Science

    Article Title: Sialyltransferase ST3 GAL6 mediates the effect of micro RNA‐26a on cell growth, migration, and invasion in hepatocellular carcinoma through the protein kinase B/mammalian target of rapamycin pathway

    doi: 10.1111/cas.13128

    Figure Lengend Snippet: Sialyltransferase ST 3 GAL 6 was upregulated in human hepatocellular carcinoma ( HCC ) tissues and cell lines. (a) Differences in expression in HCC cell lines ( MHCC 97‐H, MHCC 97‐L, and HCCLM 3) and a normal human hepatic cell line ( LO 2). (b) ST 3 GAL 6 (measured by SYBR Green quantitative real‐time PCR ) was upregulated in HCC tissues compared with the matched adjacent non‐tumorous liver tissues. The central horizontal line represents the mean value. * P

    Article Snippet: ST3GAL6 mRNA levels were quantified with SYBR Green qRT‐PCR (Takara, Otsu, Shiga, Japan) and normalized to GAPDH.

    Techniques: Expressing, SYBR Green Assay, Real-time Polymerase Chain Reaction

    Effect of the NO scavenger cPTIO on Al-induced root malate exudation and TaALMT1 expression in roots. Three-day-old seedlings were exposed to a 30 μM Al solution containing 0 or 30 μM cPTIO for 24h. ( a ) Root exudates were collected after 24h exposure and malate was analysed by HPLC. ( b ) Root apices (0–10mm) were collected. The relative expression of TaALMT1 in 10-mm root apices was determined by qRT-PCR. Means ± SD (n = 3) with different letters are significantly different at P

    Journal: Journal of Experimental Botany

    Article Title: Decreasing methylation of pectin caused by nitric oxide leads to higher aluminium binding in cell walls and greater aluminium sensitivity of wheat roots

    doi: 10.1093/jxb/erv514

    Figure Lengend Snippet: Effect of the NO scavenger cPTIO on Al-induced root malate exudation and TaALMT1 expression in roots. Three-day-old seedlings were exposed to a 30 μM Al solution containing 0 or 30 μM cPTIO for 24h. ( a ) Root exudates were collected after 24h exposure and malate was analysed by HPLC. ( b ) Root apices (0–10mm) were collected. The relative expression of TaALMT1 in 10-mm root apices was determined by qRT-PCR. Means ± SD (n = 3) with different letters are significantly different at P

    Article Snippet: The first-strand cDNA was used for SYBR Green-monitored qRT-PCR (Takara).

    Techniques: Expressing, High Performance Liquid Chromatography, Quantitative RT-PCR

    Sertoli cell markers are dysregulated in Pin1 −/− testes. ( A ) Amh (antimüllerian hormone) mRNA transcript expression was analyzed in Pin1 +/+ and Pin1 −/− testes. ( B ) Ar (androgen receptor) and ( C ) Gja1/connexin 43 mRNA levels were also analyzed in the same tissue. ( D ) The expression profile of the Sertoli cell-specific marker Wt1 (Wilms tumor) was analyzed by qRT-PCR. RNA from whole snap-frozen testicular tissue was extracted by Trizol digestion after homogenization using a mortar. qRT-PCR was performed using Sybr green reagent in an Applied Biosystems 2500 thermocycler.

    Journal: Scientific Reports

    Article Title: Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells

    doi: 10.1038/s41598-017-07229-1

    Figure Lengend Snippet: Sertoli cell markers are dysregulated in Pin1 −/− testes. ( A ) Amh (antimüllerian hormone) mRNA transcript expression was analyzed in Pin1 +/+ and Pin1 −/− testes. ( B ) Ar (androgen receptor) and ( C ) Gja1/connexin 43 mRNA levels were also analyzed in the same tissue. ( D ) The expression profile of the Sertoli cell-specific marker Wt1 (Wilms tumor) was analyzed by qRT-PCR. RNA from whole snap-frozen testicular tissue was extracted by Trizol digestion after homogenization using a mortar. qRT-PCR was performed using Sybr green reagent in an Applied Biosystems 2500 thermocycler.

    Article Snippet: To examine mRNA levels, 1 μg of total RNA was reverse transcribed using a first strand cDNA synthesis kit (Takara, Japan). qRT-PCR was conducted using the SYBR Green qRT-PCR kit (Takara) on an Applied Biosystems 2500 Real-Time PCR machine (Applied Biosystems). qRT-PCR was carried out in a 20 μl reaction mixture, which consisted of 10 μl of 2 × SYBR Green Mix, 0.2 μl of forward and reverse primers (20 μM of each primer), 0.4 μl of ROXII dye, and 2 μl diluted cDNA.

    Techniques: Expressing, Marker, Wilms Tumor Assay, Quantitative RT-PCR, Homogenization, SYBR Green Assay

    Correlations between CLIC1 and PA28B , MMP2 , MMP9 , ANXA7 , GSN , MMP12 , MMP13 , and SERPINB5 mRNA in OSCC according to qRT-PCR data. Notes: ( A ) Correlation between CLIC1 and PA28B . ( B ) Correlation between CLIC1 and MMP2 . ( C ) Correlation between CLIC1 and MMP9 . ( D ) Correlation between CLIC1 and ANXA7 . ( E ) Correlation between CLIC1 and GSN . ( F ) Correlation between CLIC1 and MMP12 . ( G ) Correlation between CLIC1 and MMP13 . ( H ) Correlation between CLIC1 and SERPINB5 . r -values calculated by Pearson correlation analysis. P -values calculated by Student’s t -test. Abbreviations: OSCC, oral squamous cell carcinoma; qRT-PCR, quantitative real-time PCR.

    Journal: OncoTargets and therapy

    Article Title: Expression of CLIC1 as a potential biomarker for oral squamous cell carcinoma: a preliminary study

    doi: 10.2147/OTT.S181936

    Figure Lengend Snippet: Correlations between CLIC1 and PA28B , MMP2 , MMP9 , ANXA7 , GSN , MMP12 , MMP13 , and SERPINB5 mRNA in OSCC according to qRT-PCR data. Notes: ( A ) Correlation between CLIC1 and PA28B . ( B ) Correlation between CLIC1 and MMP2 . ( C ) Correlation between CLIC1 and MMP9 . ( D ) Correlation between CLIC1 and ANXA7 . ( E ) Correlation between CLIC1 and GSN . ( F ) Correlation between CLIC1 and MMP12 . ( G ) Correlation between CLIC1 and MMP13 . ( H ) Correlation between CLIC1 and SERPINB5 . r -values calculated by Pearson correlation analysis. P -values calculated by Student’s t -test. Abbreviations: OSCC, oral squamous cell carcinoma; qRT-PCR, quantitative real-time PCR.

    Article Snippet: RNA was reverse-transcribed into cDNA with a ReverTra Ace qRT-PCR kit (Takara, Japan). qRT PCR was performed on a StepOnePlus RT-PCR system (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    Suppress activity of B.subtilis or surfactin. Cells were exposed to B. subtilis 168, OKB105 and surfactin in different treatments as described above. For the indicated time points, cells were collected and the yield of virus was determined by qRT-PCR ( A ) and Western blotting ( B ). (B) Lane 1, TGEV control; lane 2, virus from cells treated with 0.002 mg/ml surfactin; lane 3, virus from cells treated with 1.00E + 07 cfu/ml B. subtilis 168; lane 4, virus from cells treated with 1.00E + 07 cfu/ml B. subtilis OKB105; lane 5, mock. ( C ) Mean relative protein ratio of TGEV-N. Blots were reported with antibody to GAPDH as a loading control. The mean ± S.D. from three independent experiments are shown. Significance levels for the differences between B. subtilis and surfactin treatments and virus control from untreated cells are given above the bar: * P

    Journal: Bioscience Reports

    Article Title: Bacillus subtilis and surfactin inhibit the transmissible gastroenteritis virus from entering the intestinal epithelial cells

    doi: 10.1042/BSR20170082

    Figure Lengend Snippet: Suppress activity of B.subtilis or surfactin. Cells were exposed to B. subtilis 168, OKB105 and surfactin in different treatments as described above. For the indicated time points, cells were collected and the yield of virus was determined by qRT-PCR ( A ) and Western blotting ( B ). (B) Lane 1, TGEV control; lane 2, virus from cells treated with 0.002 mg/ml surfactin; lane 3, virus from cells treated with 1.00E + 07 cfu/ml B. subtilis 168; lane 4, virus from cells treated with 1.00E + 07 cfu/ml B. subtilis OKB105; lane 5, mock. ( C ) Mean relative protein ratio of TGEV-N. Blots were reported with antibody to GAPDH as a loading control. The mean ± S.D. from three independent experiments are shown. Significance levels for the differences between B. subtilis and surfactin treatments and virus control from untreated cells are given above the bar: * P

    Article Snippet: RNA extraction and qRT-PCR For quantitative reverse transcription-PCR (qRT-PCR), total RNA from IPEC-J2 cells was extracted using a TRIzol reagent (Life Technologies) and subjected to reverse transcription with Prime Script qRT-PCR Kit (Takara, Dalian, CA). qPCR reactions were performed in ABI 7500 instrument (Applied Biosystems, U.S.A.).

    Techniques: Activity Assay, Quantitative RT-PCR, Western Blot

    High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) qRT-PCR assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p

    Journal: Oncotarget

    Article Title: Serum long non-coding RNAs MALAT1, AFAP1-AS1 and AL359062 as diagnostic and prognostic biomarkers for nasopharyngeal carcinoma

    doi: 10.18632/oncotarget.17083

    Figure Lengend Snippet: High MALAT1, AFAP1-AS1 and AL359062 levels were related to EBV infection (A) qRT-PCR assay examined the differences of expression levels of circulating MALAT1, AFAP1-AS1 and AL359062 between EBV-positive NPC group and EBV-negative NPC group. (B) Expression levels of MALAT1, AFAP1-AS1 and AL359062 were detected in culture supernatant of EBV infected NP69 on Day 5, 10, and 15 of post-infection. EBV genes EBNA1 and LMP1 were used to measure the level of viral replication. (* p

    Article Snippet: RNA extraction and qRT-PCR RNAiso Plus reagent (TaKaRa) and RNAiso Blood reagent (TaKaRa) were applied for RNA extraction from culture supernatant and serum, respectively.

    Techniques: Infection, Quantitative RT-PCR, Expressing

    AL359062 knockdown suppress NPC cell metastasis, invasion and proliferation in vitro (A) . siRNA1+2 efficiently suppressed AL359062 expression compared with the siNC in 5-8F and CNE2 cells by qRT-PCR. (B-C) . AL359062 knockdown inhibited NPC cell migratory and invasive abilities as measured by transwell migration assay (B) and matrigel invasion assay (C). (D) . CCK-8 assay was applied to detect the effect of AL359062 knockdown on NPC cell viability. (E-F) . Forty-eight hours after AL359062 knockdown, total RNA was extracted and mRNA expression levels of metastasis and invasion-related genes CDH1, CDH2, MMP2, 3 and 9 (E), and cell cycle-related genes CDKN1B, CCND1, CCNE1, CDK2 and 4 (F) were measured by qRT-PCR. The graph summarizes the data from three independent experiments. (* p

    Journal: Oncotarget

    Article Title: Serum long non-coding RNAs MALAT1, AFAP1-AS1 and AL359062 as diagnostic and prognostic biomarkers for nasopharyngeal carcinoma

    doi: 10.18632/oncotarget.17083

    Figure Lengend Snippet: AL359062 knockdown suppress NPC cell metastasis, invasion and proliferation in vitro (A) . siRNA1+2 efficiently suppressed AL359062 expression compared with the siNC in 5-8F and CNE2 cells by qRT-PCR. (B-C) . AL359062 knockdown inhibited NPC cell migratory and invasive abilities as measured by transwell migration assay (B) and matrigel invasion assay (C). (D) . CCK-8 assay was applied to detect the effect of AL359062 knockdown on NPC cell viability. (E-F) . Forty-eight hours after AL359062 knockdown, total RNA was extracted and mRNA expression levels of metastasis and invasion-related genes CDH1, CDH2, MMP2, 3 and 9 (E), and cell cycle-related genes CDKN1B, CCND1, CCNE1, CDK2 and 4 (F) were measured by qRT-PCR. The graph summarizes the data from three independent experiments. (* p

    Article Snippet: RNA extraction and qRT-PCR RNAiso Plus reagent (TaKaRa) and RNAiso Blood reagent (TaKaRa) were applied for RNA extraction from culture supernatant and serum, respectively.

    Techniques: In Vitro, Expressing, Quantitative RT-PCR, Transwell Migration Assay, Invasion Assay, CCK-8 Assay