qrt pcr primers Thermo Fisher Search Results


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  • 90
    Thermo Fisher pcr buffer
    Differentiation of 21 bifidobacterial strains isolated from child feces using <t>rep-PCR</t> procedures. <t>DNA</t> profiles were determined in PCR reaction with (GTG) 5 primer ( a ) and BOX1R oligonucleotide ( b ). Lane: 1, DNA molecular marker, 2, Bifdobacterium NK1.2; 3, NK2.2; 4, NK6.1; 5, NK7.2; 6, NK8.1; 7, NK9.1; 8, NK10.2; 9, NK11.1; 10, NK12; 11, NK13; 12, NK14; 13, NK15; 14, NK16; 15, NK17; 16, MP1; 17, MP5; 18, MP6; 19, WP3; 20, WP4; 21, WP7; 22, WP8
    Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher reverse transcriptase polymerase chain reaction qrt pcr
    Differentiation of 21 bifidobacterial strains isolated from child feces using <t>rep-PCR</t> procedures. <t>DNA</t> profiles were determined in PCR reaction with (GTG) 5 primer ( a ) and BOX1R oligonucleotide ( b ). Lane: 1, DNA molecular marker, 2, Bifdobacterium NK1.2; 3, NK2.2; 4, NK6.1; 5, NK7.2; 6, NK8.1; 7, NK9.1; 8, NK10.2; 9, NK11.1; 10, NK12; 11, NK13; 12, NK14; 13, NK15; 14, NK16; 15, NK17; 16, MP1; 17, MP5; 18, MP6; 19, WP3; 20, WP4; 21, WP7; 22, WP8
    Reverse Transcriptase Polymerase Chain Reaction Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time polymerase chain reaction qrt pcr
    Differentiation of 21 bifidobacterial strains isolated from child feces using <t>rep-PCR</t> procedures. <t>DNA</t> profiles were determined in PCR reaction with (GTG) 5 primer ( a ) and BOX1R oligonucleotide ( b ). Lane: 1, DNA molecular marker, 2, Bifdobacterium NK1.2; 3, NK2.2; 4, NK6.1; 5, NK7.2; 6, NK8.1; 7, NK9.1; 8, NK10.2; 9, NK11.1; 10, NK12; 11, NK13; 12, NK14; 13, NK15; 14, NK16; 15, NK17; 16, MP1; 17, MP5; 18, MP6; 19, WP3; 20, WP4; 21, WP7; 22, WP8
    Real Time Polymerase Chain Reaction Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1001 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative reverse transcription polymerase chain reaction qrt pcr primers
    Differentiation of 21 bifidobacterial strains isolated from child feces using <t>rep-PCR</t> procedures. <t>DNA</t> profiles were determined in PCR reaction with (GTG) 5 primer ( a ) and BOX1R oligonucleotide ( b ). Lane: 1, DNA molecular marker, 2, Bifdobacterium NK1.2; 3, NK2.2; 4, NK6.1; 5, NK7.2; 6, NK8.1; 7, NK9.1; 8, NK10.2; 9, NK11.1; 10, NK12; 11, NK13; 12, NK14; 13, NK15; 14, NK16; 15, NK17; 16, MP1; 17, MP5; 18, MP6; 19, WP3; 20, WP4; 21, WP7; 22, WP8
    Quantitative Reverse Transcription Polymerase Chain Reaction Qrt Pcr Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time reverse transcriptase polymerase chain reaction qrt pcr
    Differentiation of 21 bifidobacterial strains isolated from child feces using <t>rep-PCR</t> procedures. <t>DNA</t> profiles were determined in PCR reaction with (GTG) 5 primer ( a ) and BOX1R oligonucleotide ( b ). Lane: 1, DNA molecular marker, 2, Bifdobacterium NK1.2; 3, NK2.2; 4, NK6.1; 5, NK7.2; 6, NK8.1; 7, NK9.1; 8, NK10.2; 9, NK11.1; 10, NK12; 11, NK13; 12, NK14; 13, NK15; 14, NK16; 15, NK17; 16, MP1; 17, MP5; 18, MP6; 19, WP3; 20, WP4; 21, WP7; 22, WP8
    Real Time Reverse Transcriptase Polymerase Chain Reaction Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher qrt pcr primers
    Th2 cytokines suppress pro-caspase-1 without affecting levels of pro-IL-1β. (A) Taqman quantitative <t>RT-PCR</t> results showing mRNA expression of pro-IL-1β in THP-1s exposed to 100 μg/mL MWCNTs with IL-4, IL-13, or IL-4 and IL-13 co-treatment. B) Taqman <t>qRT-PCR</t> results showing mRNA levels of pro-caspase-1 in THP-1s exposed to MWCNTs with IL-4, IL-13, or IL-4 and IL-13 co-treatment. Statistical analysis was performed using a one-way ANOVA with a post hoc Tukey. ***P
    Qrt Pcr Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 810 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher qrt pcr reactions
    XIST promotes tumor growth of ESCC via regulation of miR-101/EZH2 axis in vivo (A) Tumor volume of the xenografts in XIST knockdown groups and control group. (B) Tumors in nude mice formed in knockdown groups and control group were imaged. (C) Weight of dissected tumors in knockdown groups and control group was shown. Expression of XIST (D) and miR-101 (E) in the dissected tumors of knockdown groups and control group was detected by <t>qRT-PCR.</t> Immunohistochemistry staining (F) and quantification analysis of Ki-67 (G) and EZH2 in knockdown groups and control group were shown. Scale bars: 50μm. Error bars: mean ± SD, n = 6. * P
    Qrt Pcr Reactions, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6775 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman qrt pcr primers
    78Fc as an NIR optical imaging tracer in the human TEM1-expressing tumor vascular model Human (A) and mouse TEM1 mRNA (B) expression in control and hTEM1 expressing tumor grafts. TEM1 mRNA levels were evaluated with <t>qRT-PCR.</t> TEM1 expression in normal human or murine cervix was normalized to 1. 10 7 MS1-huTEM1 or control MS1 cells were injected subcutaneously into nude mice on the left or right flanks, respectively (n=3), and tumor grafts were harvested after the indicated time (2-3 weeks). huTEM1-positive tumor grafts express muTEM1 at similar levels to that of control tumor grafts. C, in vivo bioluminescence imaging (BLI) and 78Fc750- or IgG-750-based live NIR optical imaging in huTEM1-positive and control tumor grafts (n=3 per group). Green circle, MS1-TEM1 tumor; yellow circle, MS1 control tumor. D (Also in Sup videos ), 78Fc750 BLI and NIR tomographic imaging in mice grafted with MS1-huTEM1 (lower) and MS1 (upper) in nude mice. Bioluminescent signals overlap with infrared signals only in the huTEM1-positive tumors. From left, picture (area inside the purple square is used for 3D dual modality imaging to regenerate mice surface), 3D reconstructions of bioluminescence (DLIT) and NIR fluorescence (FLIT); superimposed DLIT with FLIT. n=5.
    Taqman Qrt Pcr Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ncodetm qrt pcr kit
    78Fc as an NIR optical imaging tracer in the human TEM1-expressing tumor vascular model Human (A) and mouse TEM1 mRNA (B) expression in control and hTEM1 expressing tumor grafts. TEM1 mRNA levels were evaluated with <t>qRT-PCR.</t> TEM1 expression in normal human or murine cervix was normalized to 1. 10 7 MS1-huTEM1 or control MS1 cells were injected subcutaneously into nude mice on the left or right flanks, respectively (n=3), and tumor grafts were harvested after the indicated time (2-3 weeks). huTEM1-positive tumor grafts express muTEM1 at similar levels to that of control tumor grafts. C, in vivo bioluminescence imaging (BLI) and 78Fc750- or IgG-750-based live NIR optical imaging in huTEM1-positive and control tumor grafts (n=3 per group). Green circle, MS1-TEM1 tumor; yellow circle, MS1 control tumor. D (Also in Sup videos ), 78Fc750 BLI and NIR tomographic imaging in mice grafted with MS1-huTEM1 (lower) and MS1 (upper) in nude mice. Bioluminescent signals overlap with infrared signals only in the huTEM1-positive tumors. From left, picture (area inside the purple square is used for 3D dual modality imaging to regenerate mice surface), 3D reconstructions of bioluminescence (DLIT) and NIR fluorescence (FLIT); superimposed DLIT with FLIT. n=5.
    Ncodetm Qrt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green qrt pcr
    78Fc as an NIR optical imaging tracer in the human TEM1-expressing tumor vascular model Human (A) and mouse TEM1 mRNA (B) expression in control and hTEM1 expressing tumor grafts. TEM1 mRNA levels were evaluated with <t>qRT-PCR.</t> TEM1 expression in normal human or murine cervix was normalized to 1. 10 7 MS1-huTEM1 or control MS1 cells were injected subcutaneously into nude mice on the left or right flanks, respectively (n=3), and tumor grafts were harvested after the indicated time (2-3 weeks). huTEM1-positive tumor grafts express muTEM1 at similar levels to that of control tumor grafts. C, in vivo bioluminescence imaging (BLI) and 78Fc750- or IgG-750-based live NIR optical imaging in huTEM1-positive and control tumor grafts (n=3 per group). Green circle, MS1-TEM1 tumor; yellow circle, MS1 control tumor. D (Also in Sup videos ), 78Fc750 BLI and NIR tomographic imaging in mice grafted with MS1-huTEM1 (lower) and MS1 (upper) in nude mice. Bioluminescent signals overlap with infrared signals only in the huTEM1-positive tumors. From left, picture (area inside the purple square is used for 3D dual modality imaging to regenerate mice surface), 3D reconstructions of bioluminescence (DLIT) and NIR fluorescence (FLIT); superimposed DLIT with FLIT. n=5.
    Sybr Green Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman qrt pcr system
    78Fc as an NIR optical imaging tracer in the human TEM1-expressing tumor vascular model Human (A) and mouse TEM1 mRNA (B) expression in control and hTEM1 expressing tumor grafts. TEM1 mRNA levels were evaluated with <t>qRT-PCR.</t> TEM1 expression in normal human or murine cervix was normalized to 1. 10 7 MS1-huTEM1 or control MS1 cells were injected subcutaneously into nude mice on the left or right flanks, respectively (n=3), and tumor grafts were harvested after the indicated time (2-3 weeks). huTEM1-positive tumor grafts express muTEM1 at similar levels to that of control tumor grafts. C, in vivo bioluminescence imaging (BLI) and 78Fc750- or IgG-750-based live NIR optical imaging in huTEM1-positive and control tumor grafts (n=3 per group). Green circle, MS1-TEM1 tumor; yellow circle, MS1 control tumor. D (Also in Sup videos ), 78Fc750 BLI and NIR tomographic imaging in mice grafted with MS1-huTEM1 (lower) and MS1 (upper) in nude mice. Bioluminescent signals overlap with infrared signals only in the huTEM1-positive tumors. From left, picture (area inside the purple square is used for 3D dual modality imaging to regenerate mice surface), 3D reconstructions of bioluminescence (DLIT) and NIR fluorescence (FLIT); superimposed DLIT with FLIT. n=5.
    Taqman Qrt Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher qrt pcr machine
    78Fc as an NIR optical imaging tracer in the human TEM1-expressing tumor vascular model Human (A) and mouse TEM1 mRNA (B) expression in control and hTEM1 expressing tumor grafts. TEM1 mRNA levels were evaluated with <t>qRT-PCR.</t> TEM1 expression in normal human or murine cervix was normalized to 1. 10 7 MS1-huTEM1 or control MS1 cells were injected subcutaneously into nude mice on the left or right flanks, respectively (n=3), and tumor grafts were harvested after the indicated time (2-3 weeks). huTEM1-positive tumor grafts express muTEM1 at similar levels to that of control tumor grafts. C, in vivo bioluminescence imaging (BLI) and 78Fc750- or IgG-750-based live NIR optical imaging in huTEM1-positive and control tumor grafts (n=3 per group). Green circle, MS1-TEM1 tumor; yellow circle, MS1 control tumor. D (Also in Sup videos ), 78Fc750 BLI and NIR tomographic imaging in mice grafted with MS1-huTEM1 (lower) and MS1 (upper) in nude mice. Bioluminescent signals overlap with infrared signals only in the huTEM1-positive tumors. From left, picture (area inside the purple square is used for 3D dual modality imaging to regenerate mice surface), 3D reconstructions of bioluminescence (DLIT) and NIR fluorescence (FLIT); superimposed DLIT with FLIT. n=5.
    Qrt Pcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher custom designed qrt pcr primers
    78Fc as an NIR optical imaging tracer in the human TEM1-expressing tumor vascular model Human (A) and mouse TEM1 mRNA (B) expression in control and hTEM1 expressing tumor grafts. TEM1 mRNA levels were evaluated with <t>qRT-PCR.</t> TEM1 expression in normal human or murine cervix was normalized to 1. 10 7 MS1-huTEM1 or control MS1 cells were injected subcutaneously into nude mice on the left or right flanks, respectively (n=3), and tumor grafts were harvested after the indicated time (2-3 weeks). huTEM1-positive tumor grafts express muTEM1 at similar levels to that of control tumor grafts. C, in vivo bioluminescence imaging (BLI) and 78Fc750- or IgG-750-based live NIR optical imaging in huTEM1-positive and control tumor grafts (n=3 per group). Green circle, MS1-TEM1 tumor; yellow circle, MS1 control tumor. D (Also in Sup videos ), 78Fc750 BLI and NIR tomographic imaging in mice grafted with MS1-huTEM1 (lower) and MS1 (upper) in nude mice. Bioluminescent signals overlap with infrared signals only in the huTEM1-positive tumors. From left, picture (area inside the purple square is used for 3D dual modality imaging to regenerate mice surface), 3D reconstructions of bioluminescence (DLIT) and NIR fluorescence (FLIT); superimposed DLIT with FLIT. n=5.
    Custom Designed Qrt Pcr Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mir 30b 5p qrt pcr primer
    <t>miR-30b-5p</t> expression in the gliomas. Taqman <t>qRT-PCR</t> analysis of miR-30b-5p expression in 13 cases of gliomas and paired normal brain tissues. U6 was used as an internal reference. P
    Mir 30b 5p Qrt Pcr Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher real time qrt pcr plate
    Semi-quantitative <t>RT-PCR</t> and real-time <t>qRT-PCR</t> for levels of expression of pre-miR-138 in human malignant neuroblastoma SK-N-DZ and SK-N-BE2 cells. Treatments: transfection with plasmid vector carrying scrambled shRNA (1 μg/ml) for 12 h, transfection with plasmid vector carrying hTERT shRNA (1 μg/ml) for 12 h, 100 μM APG for 24 h, and hTERT shRNA (1 μg/ml) for 12 h + 100 μM APG for 24 h. (A) Semi-quantitative RT-PCR for levels of expression of miR-138. Expression of U6 RNA was used as a loading control. The RT-PCR products were resolved on 2.2% agarose gels by electrophoresis. (B) Real-time qRT-PCR for relative levels of expression of miR-138 after normalizing with U6 RNA (control). Difference between scrambled shRNA and a monotherapy or combination therapy was considered significant at*p
    Real Time Qrt Pcr Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher stem loop qrt pcr primers
    <t>qRT-PCR</t> validation of miRs differentially expressed in whole blood samples from acute KD and adenovirus-infected patients. qRT-PCR confirmed differential expression of miR-145 in an independent cohort of 16 acute KD subjects and 14 acute adenovirus-infected controls. Red dot: subjects with coronary artery aneurysm; Blue dot: subjects with dilated coronary arteries; Black dot: subjects with normal coronary arteries. The Mann Whitney test was used to obtain p -values. NS = not significant.
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    Thermo Fisher absolute qrt pcr primers
    Expression-Profile Measurement of Tree shrew GAPDH Gene. Three pair of primers for <t>qRT-PCR</t> were designed to measure tsGAPDH mRNA expression in various tree shrew tissues. Data are shown in a point chart and grouped according to ANOVA (p
    Absolute Qrt Pcr Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher qrt pcr primers tfap2b
    Expression-Profile Measurement of Tree shrew GAPDH Gene. Three pair of primers for <t>qRT-PCR</t> were designed to measure tsGAPDH mRNA expression in various tree shrew tissues. Data are shown in a point chart and grouped according to ANOVA (p
    Qrt Pcr Primers Tfap2b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression-Profile Measurement of Tree shrew GAPDH Gene. Three pair of primers for <t>qRT-PCR</t> were designed to measure tsGAPDH mRNA expression in various tree shrew tissues. Data are shown in a point chart and grouped according to ANOVA (p
    Absolute Qrt Pcr Sybr Green Rox Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression-Profile Measurement of Tree shrew GAPDH Gene. Three pair of primers for <t>qRT-PCR</t> were designed to measure tsGAPDH mRNA expression in various tree shrew tissues. Data are shown in a point chart and grouped according to ANOVA (p
    Cellsdirect One Step Qrt Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hsa mir 210 qrt pcr primer
    Expression-Profile Measurement of Tree shrew GAPDH Gene. Three pair of primers for <t>qRT-PCR</t> were designed to measure tsGAPDH mRNA expression in various tree shrew tissues. Data are shown in a point chart and grouped according to ANOVA (p
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    Up-regulation of <t>miR-21</t> by miR-21 mimic transfection increased VEGF mRNA expression in GSCs The purified GSCs were transfected with miR-21 for 48 hrs, and the levels of miR-21 and VEGF were determined by <t>qRT-PCR.</t> ( A – B ) the levels of miR-21 and VEGF in different types of GSCs; * p
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    Thermo Fisher mir vana qrt pcr primer
    Effect of formalin fixation on miRNA expression. ( A ) Representative RNA agarose gel image (lane 1 , RNA ladder; lane 2 , fresh frozen sample; lane 3 , FFPE sample). ( B ) The expression of hsa - miR-16 and RUN6B was analyzed using real-time <t>QRT-PCR</t> analysis
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    Thermo Fisher fibronectin qrt pcr primers
    Comparison of <t>fibronectin</t> mRNA and protein levels: A) <t>QRT-PCR</t> of RNA extracted from Null and Null + cells. B) Null and Null + cells were plated and cultured overnight. Cell lystates were harvested in RIPA buffer and conditioned medium (CM) was collected.
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    Thermo Fisher real time qrt pcr primer
    Comparison of <t>fibronectin</t> mRNA and protein levels: A) <t>QRT-PCR</t> of RNA extracted from Null and Null + cells. B) Null and Null + cells were plated and cultured overnight. Cell lystates were harvested in RIPA buffer and conditioned medium (CM) was collected.
    Real Time Qrt Pcr Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Differentiation of 21 bifidobacterial strains isolated from child feces using rep-PCR procedures. DNA profiles were determined in PCR reaction with (GTG) 5 primer ( a ) and BOX1R oligonucleotide ( b ). Lane: 1, DNA molecular marker, 2, Bifdobacterium NK1.2; 3, NK2.2; 4, NK6.1; 5, NK7.2; 6, NK8.1; 7, NK9.1; 8, NK10.2; 9, NK11.1; 10, NK12; 11, NK13; 12, NK14; 13, NK15; 14, NK16; 15, NK17; 16, MP1; 17, MP5; 18, MP6; 19, WP3; 20, WP4; 21, WP7; 22, WP8

    Journal: BMC Microbiology

    Article Title: Comparison of various molecular methods for rapid differentiation of intestinal bifidobacteria at the species, subspecies and strain level

    doi: 10.1186/s12866-016-0779-3

    Figure Lengend Snippet: Differentiation of 21 bifidobacterial strains isolated from child feces using rep-PCR procedures. DNA profiles were determined in PCR reaction with (GTG) 5 primer ( a ) and BOX1R oligonucleotide ( b ). Lane: 1, DNA molecular marker, 2, Bifdobacterium NK1.2; 3, NK2.2; 4, NK6.1; 5, NK7.2; 6, NK8.1; 7, NK9.1; 8, NK10.2; 9, NK11.1; 10, NK12; 11, NK13; 12, NK14; 13, NK15; 14, NK16; 15, NK17; 16, MP1; 17, MP5; 18, MP6; 19, WP3; 20, WP4; 21, WP7; 22, WP8

    Article Snippet: PCR was carried out in the total volume of 20 μl of the reaction mixture containing 1 U (for BOXA1R-PCR) and 2 U (for (GTG)5 -PCR) of Taq DNA polymerase, 200 μM of each deoxynucleoside triphosphate, 1 μM of each primer, 50 ng of bacterial DNA and PCR buffer (Thermo Fisher Scientific, Waltham, USA).

    Techniques: Isolation, Polymerase Chain Reaction, Marker

    Randomly amplified polymorphic DNA (RAPD)-PCR patterns obtained with PER1 primer for 17 bifidobacterial strains. Analysis of the discriminatory power of the procedure applied was performed at a species level ( a ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. animalis NRRL B-41406; 4, B. bifidum DSM 204564; 5, B. breve DSM 20091; 6, B. catenulatum DSM 20224; 7, B. longum NRRL B-41409; 8, B. pseudocatenulatum DSM 20439; 9, B. pseudolongum DSM 20099; at a subspecies level ( b ) - 1, DNA molecular marker; 2, B. animalis subsp. animalis NRRL B-41406; 3, B. animalis subsp. lactis NRRL B-41405; 4, B. longum subsp. infantis ATCC 15697; 5, B. longum subsp. longum NRRL B-41409; 5, B. longum subsp. suis NRRL B-41407; 6, B. pseudolongum subsp. pseudolongum DSM 20099; 7, B. pseudolongum subsp. globosum DSM 20092; and at a strain level ( c ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. adolescentis DSM 20083; 4, B. adolescentis 20086; 5, B. breve DSM 20091; 6, B. breve NRRL B-41408; 7 , B. pseudolongum DSM 20099; 8, B. pseudolongum 20094; 9, B. pseudolongum DSM 20095

    Journal: BMC Microbiology

    Article Title: Comparison of various molecular methods for rapid differentiation of intestinal bifidobacteria at the species, subspecies and strain level

    doi: 10.1186/s12866-016-0779-3

    Figure Lengend Snippet: Randomly amplified polymorphic DNA (RAPD)-PCR patterns obtained with PER1 primer for 17 bifidobacterial strains. Analysis of the discriminatory power of the procedure applied was performed at a species level ( a ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. animalis NRRL B-41406; 4, B. bifidum DSM 204564; 5, B. breve DSM 20091; 6, B. catenulatum DSM 20224; 7, B. longum NRRL B-41409; 8, B. pseudocatenulatum DSM 20439; 9, B. pseudolongum DSM 20099; at a subspecies level ( b ) - 1, DNA molecular marker; 2, B. animalis subsp. animalis NRRL B-41406; 3, B. animalis subsp. lactis NRRL B-41405; 4, B. longum subsp. infantis ATCC 15697; 5, B. longum subsp. longum NRRL B-41409; 5, B. longum subsp. suis NRRL B-41407; 6, B. pseudolongum subsp. pseudolongum DSM 20099; 7, B. pseudolongum subsp. globosum DSM 20092; and at a strain level ( c ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. adolescentis DSM 20083; 4, B. adolescentis 20086; 5, B. breve DSM 20091; 6, B. breve NRRL B-41408; 7 , B. pseudolongum DSM 20099; 8, B. pseudolongum 20094; 9, B. pseudolongum DSM 20095

    Article Snippet: PCR was carried out in the total volume of 20 μl of the reaction mixture containing 1 U (for BOXA1R-PCR) and 2 U (for (GTG)5 -PCR) of Taq DNA polymerase, 200 μM of each deoxynucleoside triphosphate, 1 μM of each primer, 50 ng of bacterial DNA and PCR buffer (Thermo Fisher Scientific, Waltham, USA).

    Techniques: Amplification, Polymerase Chain Reaction, Marker

    BOX-PCR DNA profiles obtained for Bifidobacterium strains used in this work. Analysis of the discriminatory power of this procedure was performed at a species level ( a ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. animalis NRRL B-41406; 4, B. bifidum DSM 204564; 5, B. breve DSM 20091; 6, B. catenulatum DSM 20224; 7, B. longum NRRL B-41409; 8, B. pseudocatenulatum DSM 20439; 9, B. pseudolongum DSM 20099; at a subspecies level ( b ) - 1, DNA molecular marker; 2, B. animalis subsp. animalis NRRL B-41406; 3, B. animalis subsp. lactis NRRL B-41405; 4, B. longum subsp. infantis ATCC 15697; 5, B. longum subsp. longum NRRL B-41409; 5, B. longum subsp. suis NRRL B-41407; 6, B. pseudolongum subsp. pseudolongum DSM 20099; 7, B. pseudolongum subsp. globosum DSM 20092; and at a strain level ( c ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. adolescentis DSM 20083; 4, B. adolescentis 20086; 5, B. breve DSM 20091; 6, B. breve NRRL B-41408; 7 , B. pseudolongum DSM 20099; 8, B. pseudolongum 20094; 9, B. pseudolongum DSM 20095

    Journal: BMC Microbiology

    Article Title: Comparison of various molecular methods for rapid differentiation of intestinal bifidobacteria at the species, subspecies and strain level

    doi: 10.1186/s12866-016-0779-3

    Figure Lengend Snippet: BOX-PCR DNA profiles obtained for Bifidobacterium strains used in this work. Analysis of the discriminatory power of this procedure was performed at a species level ( a ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. animalis NRRL B-41406; 4, B. bifidum DSM 204564; 5, B. breve DSM 20091; 6, B. catenulatum DSM 20224; 7, B. longum NRRL B-41409; 8, B. pseudocatenulatum DSM 20439; 9, B. pseudolongum DSM 20099; at a subspecies level ( b ) - 1, DNA molecular marker; 2, B. animalis subsp. animalis NRRL B-41406; 3, B. animalis subsp. lactis NRRL B-41405; 4, B. longum subsp. infantis ATCC 15697; 5, B. longum subsp. longum NRRL B-41409; 5, B. longum subsp. suis NRRL B-41407; 6, B. pseudolongum subsp. pseudolongum DSM 20099; 7, B. pseudolongum subsp. globosum DSM 20092; and at a strain level ( c ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. adolescentis DSM 20083; 4, B. adolescentis 20086; 5, B. breve DSM 20091; 6, B. breve NRRL B-41408; 7 , B. pseudolongum DSM 20099; 8, B. pseudolongum 20094; 9, B. pseudolongum DSM 20095

    Article Snippet: PCR was carried out in the total volume of 20 μl of the reaction mixture containing 1 U (for BOXA1R-PCR) and 2 U (for (GTG)5 -PCR) of Taq DNA polymerase, 200 μM of each deoxynucleoside triphosphate, 1 μM of each primer, 50 ng of bacterial DNA and PCR buffer (Thermo Fisher Scientific, Waltham, USA).

    Techniques: Polymerase Chain Reaction, Marker

    (GTG) 5 -PCR patterns of 17 strains belonging to the genus Bifidobacterium . Analysis of the discriminatory power of the procedure applied was performed at a species level ( a ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. animalis NRRL B-41406; 4, B. bifidum DSM 204564; 5, B. breve DSM 20091; 6, B. catenulatum DSM 20224; 7, B. longum NRRL B-41409; 8, B. pseudocatenulatum DSM 20439; 9, B. pseudolongum DSM 20099; at a subspecies level ( b ) - 1, DNA molecular marker; 2, B. animalis subsp. animalis NRRL B-41406; 3, B. animalis subsp. lactis NRRL B-41405; 4, B. longum subsp. infantis ATCC 15697; 5, B. longum subsp. longum NRRL B-41409; 5, B. longum subsp. suis NRRL B-41407; 6, B. pseudolongum subsp. pseudolongum DSM 20099; 7, B. pseudolongum subsp. globosum DSM 20092; and at a strain level ( c ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. adolescentis DSM 20083; 4, B. adolescentis 20086; 5, B. breve DSM 20091; 6, B. breve NRRL B-41408; 7 , B. pseudolongum DSM 20099; 8, B. pseudolongum 20094; 9, B. pseudolongum DSM 20095

    Journal: BMC Microbiology

    Article Title: Comparison of various molecular methods for rapid differentiation of intestinal bifidobacteria at the species, subspecies and strain level

    doi: 10.1186/s12866-016-0779-3

    Figure Lengend Snippet: (GTG) 5 -PCR patterns of 17 strains belonging to the genus Bifidobacterium . Analysis of the discriminatory power of the procedure applied was performed at a species level ( a ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. animalis NRRL B-41406; 4, B. bifidum DSM 204564; 5, B. breve DSM 20091; 6, B. catenulatum DSM 20224; 7, B. longum NRRL B-41409; 8, B. pseudocatenulatum DSM 20439; 9, B. pseudolongum DSM 20099; at a subspecies level ( b ) - 1, DNA molecular marker; 2, B. animalis subsp. animalis NRRL B-41406; 3, B. animalis subsp. lactis NRRL B-41405; 4, B. longum subsp. infantis ATCC 15697; 5, B. longum subsp. longum NRRL B-41409; 5, B. longum subsp. suis NRRL B-41407; 6, B. pseudolongum subsp. pseudolongum DSM 20099; 7, B. pseudolongum subsp. globosum DSM 20092; and at a strain level ( c ) - 1, DNA molecular marker; 2, B. adolescentis DSM 20087; 3, B. adolescentis DSM 20083; 4, B. adolescentis 20086; 5, B. breve DSM 20091; 6, B. breve NRRL B-41408; 7 , B. pseudolongum DSM 20099; 8, B. pseudolongum 20094; 9, B. pseudolongum DSM 20095

    Article Snippet: PCR was carried out in the total volume of 20 μl of the reaction mixture containing 1 U (for BOXA1R-PCR) and 2 U (for (GTG)5 -PCR) of Taq DNA polymerase, 200 μM of each deoxynucleoside triphosphate, 1 μM of each primer, 50 ng of bacterial DNA and PCR buffer (Thermo Fisher Scientific, Waltham, USA).

    Techniques: Polymerase Chain Reaction, Marker

    Th2 cytokines suppress pro-caspase-1 without affecting levels of pro-IL-1β. (A) Taqman quantitative RT-PCR results showing mRNA expression of pro-IL-1β in THP-1s exposed to 100 μg/mL MWCNTs with IL-4, IL-13, or IL-4 and IL-13 co-treatment. B) Taqman qRT-PCR results showing mRNA levels of pro-caspase-1 in THP-1s exposed to MWCNTs with IL-4, IL-13, or IL-4 and IL-13 co-treatment. Statistical analysis was performed using a one-way ANOVA with a post hoc Tukey. ***P

    Journal: PLoS ONE

    Article Title: An Allergic Lung Microenvironment Suppresses Carbon Nanotube-Induced Inflammasome Activation via STAT6-Dependent Inhibition of Caspase-1

    doi: 10.1371/journal.pone.0128888

    Figure Lengend Snippet: Th2 cytokines suppress pro-caspase-1 without affecting levels of pro-IL-1β. (A) Taqman quantitative RT-PCR results showing mRNA expression of pro-IL-1β in THP-1s exposed to 100 μg/mL MWCNTs with IL-4, IL-13, or IL-4 and IL-13 co-treatment. B) Taqman qRT-PCR results showing mRNA levels of pro-caspase-1 in THP-1s exposed to MWCNTs with IL-4, IL-13, or IL-4 and IL-13 co-treatment. Statistical analysis was performed using a one-way ANOVA with a post hoc Tukey. ***P

    Article Snippet: All qRT-PCR primers were purchased from Life Technologies.

    Techniques: Quantitative RT-PCR, Expressing

    XIST promotes tumor growth of ESCC via regulation of miR-101/EZH2 axis in vivo (A) Tumor volume of the xenografts in XIST knockdown groups and control group. (B) Tumors in nude mice formed in knockdown groups and control group were imaged. (C) Weight of dissected tumors in knockdown groups and control group was shown. Expression of XIST (D) and miR-101 (E) in the dissected tumors of knockdown groups and control group was detected by qRT-PCR. Immunohistochemistry staining (F) and quantification analysis of Ki-67 (G) and EZH2 in knockdown groups and control group were shown. Scale bars: 50μm. Error bars: mean ± SD, n = 6. * P

    Journal: Oncotarget

    Article Title: Long noncoding RNA XIST promotes malignancies of esophageal squamous cell carcinoma via regulation of miR-101/EZH2

    doi: 10.18632/oncotarget.18638

    Figure Lengend Snippet: XIST promotes tumor growth of ESCC via regulation of miR-101/EZH2 axis in vivo (A) Tumor volume of the xenografts in XIST knockdown groups and control group. (B) Tumors in nude mice formed in knockdown groups and control group were imaged. (C) Weight of dissected tumors in knockdown groups and control group was shown. Expression of XIST (D) and miR-101 (E) in the dissected tumors of knockdown groups and control group was detected by qRT-PCR. Immunohistochemistry staining (F) and quantification analysis of Ki-67 (G) and EZH2 in knockdown groups and control group were shown. Scale bars: 50μm. Error bars: mean ± SD, n = 6. * P

    Article Snippet: The primers used for qRT-PCR assays were obtained from Life Technology and summarized in .

    Techniques: In Vivo, Mouse Assay, Expressing, Quantitative RT-PCR, Immunohistochemistry, Staining

    XIST was overexpressed in ESCC tissues and correlates with adverse prognosis of ESCC patients (A) Relative XIST expression in ESCC tissues (n=127) compared with corresponding adjacent normal tissues (n=127). XIST expression was examined by qRT-PCR and normalized to GAPDH expression. Results were presented as Δcycle threshold (ΔCt) in tumor tissues relative to normal tissues. (B) Expression of XIST in ESCC cell lines (KYSE30, KYSE510, KYSE410, KYSE520, KYSE140 and KYSE150) compared with that of the immortalized esophageal epithelial cell line NE1, data was presented as expression fold change relative to NE1. (C) ESCC patients were assigned to high XIST group and low XIST group according to the fold change of XIST in tumor tissues compared with that in normal tissues. Kaplan–Meier curves indicate patients with high-level XIST expression (n=64) showed reduced overall survival time compared with patients with low-level XIST expression (n=63) ( P =0.005, log-rank test). (D) Kaplan–Meier curves indicate patients with high-level XIST expression showed reduced disease-free survival time compared with patients with low-level XIST expression ( P =0.0219, log-rank test). Error bars: mean ± SD, n = 3. * P

    Journal: Oncotarget

    Article Title: Long noncoding RNA XIST promotes malignancies of esophageal squamous cell carcinoma via regulation of miR-101/EZH2

    doi: 10.18632/oncotarget.18638

    Figure Lengend Snippet: XIST was overexpressed in ESCC tissues and correlates with adverse prognosis of ESCC patients (A) Relative XIST expression in ESCC tissues (n=127) compared with corresponding adjacent normal tissues (n=127). XIST expression was examined by qRT-PCR and normalized to GAPDH expression. Results were presented as Δcycle threshold (ΔCt) in tumor tissues relative to normal tissues. (B) Expression of XIST in ESCC cell lines (KYSE30, KYSE510, KYSE410, KYSE520, KYSE140 and KYSE150) compared with that of the immortalized esophageal epithelial cell line NE1, data was presented as expression fold change relative to NE1. (C) ESCC patients were assigned to high XIST group and low XIST group according to the fold change of XIST in tumor tissues compared with that in normal tissues. Kaplan–Meier curves indicate patients with high-level XIST expression (n=64) showed reduced overall survival time compared with patients with low-level XIST expression (n=63) ( P =0.005, log-rank test). (D) Kaplan–Meier curves indicate patients with high-level XIST expression showed reduced disease-free survival time compared with patients with low-level XIST expression ( P =0.0219, log-rank test). Error bars: mean ± SD, n = 3. * P

    Article Snippet: The primers used for qRT-PCR assays were obtained from Life Technology and summarized in .

    Techniques: Expressing, Quantitative RT-PCR

    Relative expression of eng mRNA in P. pastoris CL2 in response to cold-shock at 4 °C. CL2 cells were grown in medium BMGH (30 °C for 4 h), from OD 600 = 1 inoculum, then subjected to cold-shock for the induction of eng mRNA transcript expression (4 °C for 6 h). Samples were taken at 0, 2 and 6 h for analysis by qRT-PCR; and finally, they were transferred to 30 °C for 4 h for ENG product synthesis, stage in which the corresponding sample at 2 h was taken for analysis by qRT-PCR. The values of relative expression of eng mRNA were calculated in relation to eng and the housekeeping constitutive gene act1 (control) expression using method 2 −ΔΔCt

    Journal: AMB Express

    Article Title: Autolysis of Pichia pastoris induced by cold

    doi: 10.1186/s13568-017-0397-y

    Figure Lengend Snippet: Relative expression of eng mRNA in P. pastoris CL2 in response to cold-shock at 4 °C. CL2 cells were grown in medium BMGH (30 °C for 4 h), from OD 600 = 1 inoculum, then subjected to cold-shock for the induction of eng mRNA transcript expression (4 °C for 6 h). Samples were taken at 0, 2 and 6 h for analysis by qRT-PCR; and finally, they were transferred to 30 °C for 4 h for ENG product synthesis, stage in which the corresponding sample at 2 h was taken for analysis by qRT-PCR. The values of relative expression of eng mRNA were calculated in relation to eng and the housekeeping constitutive gene act1 (control) expression using method 2 −ΔΔCt

    Article Snippet: Primers and probes used for qRT-PCR analyses were designed by Applied Biosystems (Foster City, CA, USA; Table ).

    Techniques: Expressing, Quantitative RT-PCR

    The expression levels of JAK and STAT3 in placental tissues of PE patients and normal pregnant women. ( A ) The qRT-PCR was performed to determine the mRNA levels of JAK and STAT3; ( B ) the gray values of JAK and STAT3 protein bands; ( C ) the protein levels of JAK and STAT3 in tissues; PE – preeclampsia; qRT-PCR – quantitative real-time polymerase chain reaction; ** P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Hypoxia-Induced Activation of JAK/STAT3 Signaling Pathway Promotes Trophoblast Cell Viability and Angiogenesis in Preeclampsia

    doi: 10.12659/MSM.905418

    Figure Lengend Snippet: The expression levels of JAK and STAT3 in placental tissues of PE patients and normal pregnant women. ( A ) The qRT-PCR was performed to determine the mRNA levels of JAK and STAT3; ( B ) the gray values of JAK and STAT3 protein bands; ( C ) the protein levels of JAK and STAT3 in tissues; PE – preeclampsia; qRT-PCR – quantitative real-time polymerase chain reaction; ** P

    Article Snippet: The primers for the qRT-PCR assay was designed and synthetized by Invitrogen (Carlsbad, CA, USA) , using glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) as the internal reference.

    Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    78Fc as an NIR optical imaging tracer in the human TEM1-expressing tumor vascular model Human (A) and mouse TEM1 mRNA (B) expression in control and hTEM1 expressing tumor grafts. TEM1 mRNA levels were evaluated with qRT-PCR. TEM1 expression in normal human or murine cervix was normalized to 1. 10 7 MS1-huTEM1 or control MS1 cells were injected subcutaneously into nude mice on the left or right flanks, respectively (n=3), and tumor grafts were harvested after the indicated time (2-3 weeks). huTEM1-positive tumor grafts express muTEM1 at similar levels to that of control tumor grafts. C, in vivo bioluminescence imaging (BLI) and 78Fc750- or IgG-750-based live NIR optical imaging in huTEM1-positive and control tumor grafts (n=3 per group). Green circle, MS1-TEM1 tumor; yellow circle, MS1 control tumor. D (Also in Sup videos ), 78Fc750 BLI and NIR tomographic imaging in mice grafted with MS1-huTEM1 (lower) and MS1 (upper) in nude mice. Bioluminescent signals overlap with infrared signals only in the huTEM1-positive tumors. From left, picture (area inside the purple square is used for 3D dual modality imaging to regenerate mice surface), 3D reconstructions of bioluminescence (DLIT) and NIR fluorescence (FLIT); superimposed DLIT with FLIT. n=5.

    Journal: Oncotarget

    Article Title: Development, optimization, and validation of novel anti-TEM1/CD248 affinity agent for optical imaging in cancer

    doi:

    Figure Lengend Snippet: 78Fc as an NIR optical imaging tracer in the human TEM1-expressing tumor vascular model Human (A) and mouse TEM1 mRNA (B) expression in control and hTEM1 expressing tumor grafts. TEM1 mRNA levels were evaluated with qRT-PCR. TEM1 expression in normal human or murine cervix was normalized to 1. 10 7 MS1-huTEM1 or control MS1 cells were injected subcutaneously into nude mice on the left or right flanks, respectively (n=3), and tumor grafts were harvested after the indicated time (2-3 weeks). huTEM1-positive tumor grafts express muTEM1 at similar levels to that of control tumor grafts. C, in vivo bioluminescence imaging (BLI) and 78Fc750- or IgG-750-based live NIR optical imaging in huTEM1-positive and control tumor grafts (n=3 per group). Green circle, MS1-TEM1 tumor; yellow circle, MS1 control tumor. D (Also in Sup videos ), 78Fc750 BLI and NIR tomographic imaging in mice grafted with MS1-huTEM1 (lower) and MS1 (upper) in nude mice. Bioluminescent signals overlap with infrared signals only in the huTEM1-positive tumors. From left, picture (area inside the purple square is used for 3D dual modality imaging to regenerate mice surface), 3D reconstructions of bioluminescence (DLIT) and NIR fluorescence (FLIT); superimposed DLIT with FLIT. n=5.

    Article Snippet: Taqman qRT-PCR primers are from Applied Biosystems.

    Techniques: Optical Imaging, Expressing, Quantitative RT-PCR, Injection, Mouse Assay, In Vivo, Imaging, Fluorescence

    78Fc as an NIR optical imaging tracer in a murine TEM1-expressing lung cancer xenograft model A, Live cell ELISA analysis of 78Fc labeled with Vivotag750 via either maleimide (red) or NHS (green) linkage. Blue indicates the binding activity of unlabeled 78Fc. B, TC1-derived subcutaneous tumor grafts express high level of muTEM1. 10 4 TC1 cells were injected subcutaneously into B6 mice and tumors were harvested at the indicated time (up to 6 wks). muTEM1 mRNA levels in tumor and parental cells were measured by qRT-PCR, and 2H11 and MS1 cells were used as positive and negative controls, respectively. C, in vivo bioluminescence imaging (BLI) and 78Fc750-based live NIR optical imaging in a TC1 subcutaneous tumor model (n=5). 78Fc750 was given via retro orbital injection (5 mg/kg in 100 uL) and a longitudinal study of NIR imaging was performed up to 3 days post injection (n=5). Green circle, muTEM1+ positive tumor. 1. heart; 2.brain; 3. liver; 4. lung; 5. spleen; 6. kidney; 7. small intestine; 8. bladder; 9. ovary; 10. uterus; 11. thyroid; 12. TC1 sc. xenograft. Residual tracer was observed at the injection site (retro-orbital complexes). D, TC1 lung metastatic lesions express higher levels of muTEM1 than normal tissue in the lung. E, in vivo bioluminescence imaging (BLI) and 78Fc750-based live NIR optical imaging in a TC1 lung metastasis model. 78Fc750 was given via retro orbital injection (5 mg/kg in 100 uL) and a longitudinal study of NIR imaging was performed at 3 days post injection (n=5). From left: ventral view of mouse BLI showing TC1 lung metastasis at day0; live animal NIR at day2 p.i. of 78Fc750; ventral view at day3 p.i. (postmortem) of same animal with liver and kidneys removed to show lung signal; all harvested organs including liver and kidneys; close up view of the lung. Green box, muTEM1+ positive lung tumor. 1. heart; 2.brain; 3. liver; 4. lung; 5. spleen; 6. kidney; 7. small intestine; 8. bladder; 9. ovary; 10. uterus.

    Journal: Oncotarget

    Article Title: Development, optimization, and validation of novel anti-TEM1/CD248 affinity agent for optical imaging in cancer

    doi:

    Figure Lengend Snippet: 78Fc as an NIR optical imaging tracer in a murine TEM1-expressing lung cancer xenograft model A, Live cell ELISA analysis of 78Fc labeled with Vivotag750 via either maleimide (red) or NHS (green) linkage. Blue indicates the binding activity of unlabeled 78Fc. B, TC1-derived subcutaneous tumor grafts express high level of muTEM1. 10 4 TC1 cells were injected subcutaneously into B6 mice and tumors were harvested at the indicated time (up to 6 wks). muTEM1 mRNA levels in tumor and parental cells were measured by qRT-PCR, and 2H11 and MS1 cells were used as positive and negative controls, respectively. C, in vivo bioluminescence imaging (BLI) and 78Fc750-based live NIR optical imaging in a TC1 subcutaneous tumor model (n=5). 78Fc750 was given via retro orbital injection (5 mg/kg in 100 uL) and a longitudinal study of NIR imaging was performed up to 3 days post injection (n=5). Green circle, muTEM1+ positive tumor. 1. heart; 2.brain; 3. liver; 4. lung; 5. spleen; 6. kidney; 7. small intestine; 8. bladder; 9. ovary; 10. uterus; 11. thyroid; 12. TC1 sc. xenograft. Residual tracer was observed at the injection site (retro-orbital complexes). D, TC1 lung metastatic lesions express higher levels of muTEM1 than normal tissue in the lung. E, in vivo bioluminescence imaging (BLI) and 78Fc750-based live NIR optical imaging in a TC1 lung metastasis model. 78Fc750 was given via retro orbital injection (5 mg/kg in 100 uL) and a longitudinal study of NIR imaging was performed at 3 days post injection (n=5). From left: ventral view of mouse BLI showing TC1 lung metastasis at day0; live animal NIR at day2 p.i. of 78Fc750; ventral view at day3 p.i. (postmortem) of same animal with liver and kidneys removed to show lung signal; all harvested organs including liver and kidneys; close up view of the lung. Green box, muTEM1+ positive lung tumor. 1. heart; 2.brain; 3. liver; 4. lung; 5. spleen; 6. kidney; 7. small intestine; 8. bladder; 9. ovary; 10. uterus.

    Article Snippet: Taqman qRT-PCR primers are from Applied Biosystems.

    Techniques: Optical Imaging, Expressing, Enzyme-linked Immunosorbent Assay, Labeling, Binding Assay, Activity Assay, Derivative Assay, Injection, Mouse Assay, Quantitative RT-PCR, In Vivo, Imaging

    miR-30b-5p expression in the gliomas. Taqman qRT-PCR analysis of miR-30b-5p expression in 13 cases of gliomas and paired normal brain tissues. U6 was used as an internal reference. P

    Journal: Saudi Journal of Biological Sciences

    Article Title: MiR-30b-5p modulates glioma cell proliferation by direct targeting MTDH

    doi: 10.1016/j.sjbs.2018.02.015

    Figure Lengend Snippet: miR-30b-5p expression in the gliomas. Taqman qRT-PCR analysis of miR-30b-5p expression in 13 cases of gliomas and paired normal brain tissues. U6 was used as an internal reference. P

    Article Snippet: MiR-30b-5p qRT-PCR primer was purchased from Applied Biosystem.

    Techniques: Expressing, Quantitative RT-PCR

    Effect of miR-30b-5p on cell proliferation and migration of glioma cells. (A) Glioma cells were overexpressed miR-30b-5p or vector control (miR-NC). The expression level was detected by qRT-PCR. Data are expressed as fold change (mean ± SE). * P

    Journal: Saudi Journal of Biological Sciences

    Article Title: MiR-30b-5p modulates glioma cell proliferation by direct targeting MTDH

    doi: 10.1016/j.sjbs.2018.02.015

    Figure Lengend Snippet: Effect of miR-30b-5p on cell proliferation and migration of glioma cells. (A) Glioma cells were overexpressed miR-30b-5p or vector control (miR-NC). The expression level was detected by qRT-PCR. Data are expressed as fold change (mean ± SE). * P

    Article Snippet: MiR-30b-5p qRT-PCR primer was purchased from Applied Biosystem.

    Techniques: Migration, Plasmid Preparation, Expressing, Quantitative RT-PCR

    Semi-quantitative RT-PCR and real-time qRT-PCR for levels of expression of pre-miR-138 in human malignant neuroblastoma SK-N-DZ and SK-N-BE2 cells. Treatments: transfection with plasmid vector carrying scrambled shRNA (1 μg/ml) for 12 h, transfection with plasmid vector carrying hTERT shRNA (1 μg/ml) for 12 h, 100 μM APG for 24 h, and hTERT shRNA (1 μg/ml) for 12 h + 100 μM APG for 24 h. (A) Semi-quantitative RT-PCR for levels of expression of miR-138. Expression of U6 RNA was used as a loading control. The RT-PCR products were resolved on 2.2% agarose gels by electrophoresis. (B) Real-time qRT-PCR for relative levels of expression of miR-138 after normalizing with U6 RNA (control). Difference between scrambled shRNA and a monotherapy or combination therapy was considered significant at*p

    Journal: Experimental cell research

    Article Title: miR-138 overexpression is more powerful than hTERT knockdown to potentiate apigenin for apoptosis in neuroblastoma in vitro and in vivo

    doi: 10.1016/j.yexcr.2013.02.025

    Figure Lengend Snippet: Semi-quantitative RT-PCR and real-time qRT-PCR for levels of expression of pre-miR-138 in human malignant neuroblastoma SK-N-DZ and SK-N-BE2 cells. Treatments: transfection with plasmid vector carrying scrambled shRNA (1 μg/ml) for 12 h, transfection with plasmid vector carrying hTERT shRNA (1 μg/ml) for 12 h, 100 μM APG for 24 h, and hTERT shRNA (1 μg/ml) for 12 h + 100 μM APG for 24 h. (A) Semi-quantitative RT-PCR for levels of expression of miR-138. Expression of U6 RNA was used as a loading control. The RT-PCR products were resolved on 2.2% agarose gels by electrophoresis. (B) Real-time qRT-PCR for relative levels of expression of miR-138 after normalizing with U6 RNA (control). Difference between scrambled shRNA and a monotherapy or combination therapy was considered significant at*p

    Article Snippet: Master mix (3 μl) containing all of the reaction components except the primers were dispensed into a real-time qRT-PCR plate (Life Technologies, Grand Island, NY, USA).

    Techniques: Quantitative RT-PCR, Expressing, Transfection, Plasmid Preparation, shRNA, Reverse Transcription Polymerase Chain Reaction, Electrophoresis

    qRT-PCR validation of miRs differentially expressed in whole blood samples from acute KD and adenovirus-infected patients. qRT-PCR confirmed differential expression of miR-145 in an independent cohort of 16 acute KD subjects and 14 acute adenovirus-infected controls. Red dot: subjects with coronary artery aneurysm; Blue dot: subjects with dilated coronary arteries; Black dot: subjects with normal coronary arteries. The Mann Whitney test was used to obtain p -values. NS = not significant.

    Journal: PLoS ONE

    Article Title: Differential Expression of miR-145 in Children with Kawasaki Disease

    doi: 10.1371/journal.pone.0058159

    Figure Lengend Snippet: qRT-PCR validation of miRs differentially expressed in whole blood samples from acute KD and adenovirus-infected patients. qRT-PCR confirmed differential expression of miR-145 in an independent cohort of 16 acute KD subjects and 14 acute adenovirus-infected controls. Red dot: subjects with coronary artery aneurysm; Blue dot: subjects with dilated coronary arteries; Black dot: subjects with normal coronary arteries. The Mann Whitney test was used to obtain p -values. NS = not significant.

    Article Snippet: qRT-PCR To validate differentially expressed miRs, qRT-PCR was performed using commercially available stem-loop qRT-PCR primers (Life Technologies, Grand Island, NY) for miR-23a (Assay ID 000399), miR-145 (Assay ID 002278), miR-199b-5p (Assay ID 000580), miR-223 (Assay ID 002295), miR-618 (Assay ID 001593), and miR-1271 (Assay ID 002779).

    Techniques: Quantitative RT-PCR, Infection, Expressing, MANN-WHITNEY

    qRT-PCR validation of miRs differentially expressed in acute and convalescent KD whole blood samples. qRT-PCR confirmed differential expression between acute and convalescent blood samples for six of eight miRs. Red line: subjects with coronary artery aneurysm, Blue line: subjects with transiently dilated coronary arteries, Black line: subjects with normal coronary arteries. Comparisons by the Wilcoxon signed rank test. *denotes complementary sequence. NS = not significant.

    Journal: PLoS ONE

    Article Title: Differential Expression of miR-145 in Children with Kawasaki Disease

    doi: 10.1371/journal.pone.0058159

    Figure Lengend Snippet: qRT-PCR validation of miRs differentially expressed in acute and convalescent KD whole blood samples. qRT-PCR confirmed differential expression between acute and convalescent blood samples for six of eight miRs. Red line: subjects with coronary artery aneurysm, Blue line: subjects with transiently dilated coronary arteries, Black line: subjects with normal coronary arteries. Comparisons by the Wilcoxon signed rank test. *denotes complementary sequence. NS = not significant.

    Article Snippet: qRT-PCR To validate differentially expressed miRs, qRT-PCR was performed using commercially available stem-loop qRT-PCR primers (Life Technologies, Grand Island, NY) for miR-23a (Assay ID 000399), miR-145 (Assay ID 002278), miR-199b-5p (Assay ID 000580), miR-223 (Assay ID 002295), miR-618 (Assay ID 001593), and miR-1271 (Assay ID 002779).

    Techniques: Quantitative RT-PCR, Expressing, Sequencing

    Extracelluar vesicles isolated from the plasma of acute KD subjects. a. Size distribution of extracellular vesicles from plasma. b. miR-145 analysis in extracellular vesicles. Vesicles isolated from whole blood from 14 acute KD subjects were pooled and divided into 5 treatment groups. qRT-PCR levels were compared to the “no treatment” sample.

    Journal: PLoS ONE

    Article Title: Differential Expression of miR-145 in Children with Kawasaki Disease

    doi: 10.1371/journal.pone.0058159

    Figure Lengend Snippet: Extracelluar vesicles isolated from the plasma of acute KD subjects. a. Size distribution of extracellular vesicles from plasma. b. miR-145 analysis in extracellular vesicles. Vesicles isolated from whole blood from 14 acute KD subjects were pooled and divided into 5 treatment groups. qRT-PCR levels were compared to the “no treatment” sample.

    Article Snippet: qRT-PCR To validate differentially expressed miRs, qRT-PCR was performed using commercially available stem-loop qRT-PCR primers (Life Technologies, Grand Island, NY) for miR-23a (Assay ID 000399), miR-145 (Assay ID 002278), miR-199b-5p (Assay ID 000580), miR-223 (Assay ID 002295), miR-618 (Assay ID 001593), and miR-1271 (Assay ID 002779).

    Techniques: Isolation, Quantitative RT-PCR

    Expression-Profile Measurement of Tree shrew GAPDH Gene. Three pair of primers for qRT-PCR were designed to measure tsGAPDH mRNA expression in various tree shrew tissues. Data are shown in a point chart and grouped according to ANOVA (p

    Journal: PLoS ONE

    Article Title: Identification of Glyceraldehyde 3-Phosphate Dehydrogenase Sequence and Expression Profiles in Tree Shrew (Tupaia belangeri)

    doi: 10.1371/journal.pone.0098552

    Figure Lengend Snippet: Expression-Profile Measurement of Tree shrew GAPDH Gene. Three pair of primers for qRT-PCR were designed to measure tsGAPDH mRNA expression in various tree shrew tissues. Data are shown in a point chart and grouped according to ANOVA (p

    Article Snippet: Construction of Standard Plasmid for Absolute qRT-PCR Primers ( ) were designed and based on the sequence (GenBank KC215182) then synthesized (Life Technologies) in order to amplify the fragment to construct standard plasmid.

    Techniques: Expressing, Quantitative RT-PCR

    Up-regulation of miR-21 by miR-21 mimic transfection increased VEGF mRNA expression in GSCs The purified GSCs were transfected with miR-21 for 48 hrs, and the levels of miR-21 and VEGF were determined by qRT-PCR. ( A – B ) the levels of miR-21 and VEGF in different types of GSCs; * p

    Journal: Oncotarget

    Article Title: Glioma stem cells-derived exosomes promote the angiogenic ability of endothelial cells through miR-21/VEGF signal

    doi: 10.18632/oncotarget.16661

    Figure Lengend Snippet: Up-regulation of miR-21 by miR-21 mimic transfection increased VEGF mRNA expression in GSCs The purified GSCs were transfected with miR-21 for 48 hrs, and the levels of miR-21 and VEGF were determined by qRT-PCR. ( A – B ) the levels of miR-21 and VEGF in different types of GSCs; * p

    Article Snippet: For detecting miR-21 level, reverse transcription (RT) reactions were performed by using mirVana qRT-PCR miRNA Detection Kit and hsa-miR-21 qRT-PCR primer set from Ambion.

    Techniques: Transfection, Expressing, Purification, Quantitative RT-PCR

    Expression of miR-21 , p47 phox , PDCD4, and maspin in human prostate cancer specimens. Expression of miR-21 (A) and p47 phox (B) was determined in the human prostate cancer RNA panel obtained from Origene Technologies. qRT-PCR studies show high expression

    Journal: Antioxidants & Redox Signaling

    Article Title: Essential Role of NADPH Oxidase-Dependent Reactive Oxygen Species Generation in Regulating MicroRNA-21 Expression and Function in Prostate Cancer

    doi: 10.1089/ars.2012.4820

    Figure Lengend Snippet: Expression of miR-21 , p47 phox , PDCD4, and maspin in human prostate cancer specimens. Expression of miR-21 (A) and p47 phox (B) was determined in the human prostate cancer RNA panel obtained from Origene Technologies. qRT-PCR studies show high expression

    Article Snippet: Total RNA was isolated from PC-3M-MM2 cells using QIAzol Lysis Reagent (Qiagen, Valencia, CA) as directed by the manufacturer. miR-21 cDNA was generated from 200 ng of total RNA, which was reverse transcribed using hsa-miR-21 qRT-PCR primer set (Applied Biosystems, Foster City, CA) and TaqMan MicroRNA Reverse Transcription (RT) kit (Applied Biosystems).

    Techniques: Expressing, Quantitative RT-PCR

    Effect of formalin fixation on miRNA expression. ( A ) Representative RNA agarose gel image (lane 1 , RNA ladder; lane 2 , fresh frozen sample; lane 3 , FFPE sample). ( B ) The expression of hsa - miR-16 and RUN6B was analyzed using real-time QRT-PCR analysis

    Journal:

    Article Title: Systematic analysis of microRNA expression of RNA extracted from fresh frozen and formalin-fixed paraffin-embedded samples

    doi: 10.1261/rna.642907

    Figure Lengend Snippet: Effect of formalin fixation on miRNA expression. ( A ) Representative RNA agarose gel image (lane 1 , RNA ladder; lane 2 , fresh frozen sample; lane 3 , FFPE sample). ( B ) The expression of hsa - miR-16 and RUN6B was analyzed using real-time QRT-PCR analysis

    Article Snippet: mir Vana qRT-PCR Primer Sets (Ambion Inc.) for miRNA specific reverse transcription including hsa-miR-181b endogenous control 5S rRNA were utilized according to the manufacturer's protocol.

    Techniques: Expressing, Agarose Gel Electrophoresis, Formalin-fixed Paraffin-Embedded, Quantitative RT-PCR

    Expression of miR-21 in renal cancer cells. (A – C) Total RNAs from HK2 proximal tubular epithelial cells and ACHN renal cancer cells were used to detect mature miR-21 (panel A), pre-miR-21 (panel B) and pri-miR-21 (panel C) by real time qRT-PCR as described in the Materials and Methods . Mean ± SE of 4 measurements is shown. In panels A and B, *p = 0.004 vs HK2 cells. In panel C, *p = 0.02 vs HK2 cells. (D) HK2 and ACHN cells were transfected with miR-21-Luc reporter along with Renilla null plasmid. The cell lysates were used to determine luciferase activity as described in the Materials and Methods . Mean ± SE of 6 measurements is shown. *p = 0.001 vs HK2 cells. (E) Increased phosphorylation of p65 NFkB in renal cancer cells. Lysates of HK2 and ACHN cells were immunoblotted with phospho-p65 (Ser-536), p65 and actin antibodies. (F) Mutant p65 S536A inhibits miR-21 transcription. ACHN renal cancer cells were cotransfected with miR-21-Luc and p65 S536A expression vector. The luciferase activity was determined in the cell lysates. Mean ± SE of quadruplicate measurements is shown. *p = 0.02 vs vector. Bottom panels show expression of the HA-tagged p65 S536A and actin.

    Journal: PLoS ONE

    Article Title: microRNA-21 Governs TORC1 Activation in Renal Cancer Cell Proliferation and Invasion

    doi: 10.1371/journal.pone.0037366

    Figure Lengend Snippet: Expression of miR-21 in renal cancer cells. (A – C) Total RNAs from HK2 proximal tubular epithelial cells and ACHN renal cancer cells were used to detect mature miR-21 (panel A), pre-miR-21 (panel B) and pri-miR-21 (panel C) by real time qRT-PCR as described in the Materials and Methods . Mean ± SE of 4 measurements is shown. In panels A and B, *p = 0.004 vs HK2 cells. In panel C, *p = 0.02 vs HK2 cells. (D) HK2 and ACHN cells were transfected with miR-21-Luc reporter along with Renilla null plasmid. The cell lysates were used to determine luciferase activity as described in the Materials and Methods . Mean ± SE of 6 measurements is shown. *p = 0.001 vs HK2 cells. (E) Increased phosphorylation of p65 NFkB in renal cancer cells. Lysates of HK2 and ACHN cells were immunoblotted with phospho-p65 (Ser-536), p65 and actin antibodies. (F) Mutant p65 S536A inhibits miR-21 transcription. ACHN renal cancer cells were cotransfected with miR-21-Luc and p65 S536A expression vector. The luciferase activity was determined in the cell lysates. Mean ± SE of quadruplicate measurements is shown. *p = 0.02 vs vector. Bottom panels show expression of the HA-tagged p65 S536A and actin.

    Article Snippet: The PCR conditions for amplifying pri-miR-21 were identical to those for pre-miR-21 except annealing was performed at 54°C. mirVana qRT-PCR primer sets for U6 (Ambion) were used for normalization.

    Techniques: Expressing, Quantitative RT-PCR, Transfection, Plasmid Preparation, Luciferase, Activity Assay, Mutagenesis

    Comparison of fibronectin mRNA and protein levels: A) QRT-PCR of RNA extracted from Null and Null + cells. B) Null and Null + cells were plated and cultured overnight. Cell lystates were harvested in RIPA buffer and conditioned medium (CM) was collected.

    Journal: Experimental cell research

    Article Title: PLC-?1 Regulates Fibronectin Assembly and Cell Aggregation

    doi: 10.1016/j.yexcr.2009.04.008

    Figure Lengend Snippet: Comparison of fibronectin mRNA and protein levels: A) QRT-PCR of RNA extracted from Null and Null + cells. B) Null and Null + cells were plated and cultured overnight. Cell lystates were harvested in RIPA buffer and conditioned medium (CM) was collected.

    Article Snippet: Fibronectin QRT-PCR primers were purchased from Applied Biosystems. cDNA was generated using the iscript cDNA Synthesis Kit (BioRad).

    Techniques: Quantitative RT-PCR, Cell Culture

    Reduction of miR-195 level in both 2VO rats and patients with VaD. A , Sequences of the miR-195/15b/16 family members. The conserved seed sequences among three miRNAs are underlined. B , miR-195 levels after normalization to U6 levels, measured by qRT-PCR. * p

    Journal: The Journal of Neuroscience

    Article Title: MicroRNA-195 Protects Against Dementia Induced by Chronic Brain Hypoperfusion via Its Anti-Amyloidogenic Effect in Rats

    doi: 10.1523/JNEUROSCI.1997-12.2013

    Figure Lengend Snippet: Reduction of miR-195 level in both 2VO rats and patients with VaD. A , Sequences of the miR-195/15b/16 family members. The conserved seed sequences among three miRNAs are underlined. B , miR-195 levels after normalization to U6 levels, measured by qRT-PCR. * p

    Article Snippet: The TaqMan qRT-PCR probes and primers for miR-195 (catalog #4427975, ID: 000494), BACE1 (catalog #4331182; ID: Rn00569988_m1), APP (catalog #448892; ID: Rn00570673_ml), U6 (catalog #4427975; ID: 001973), and β-actin (catalog #4331182, ID: Rn00667869_ml) mRNA levels were designed by Applied Biosystems. qPCR was performed on a thermocycler ABI Prism 7500 fast (Applied Biosystems), and the protocol was as follows: (1) 95°C, 10 min; (2) 95°C, 15 s; (3) 60°C, and 1 min (repeat (2) and (3) for 40 cycles).

    Techniques: Quantitative RT-PCR

    miR-195 knockdown produces learning and memory deficits in rats. A , Verification of the injection sites by Evans blue staining in stereotaxic surgery. B , Quantification of miR-195 in the hippocampus and the cortex tissues after stereotaxic injection for 8 weeks using qRT-PCR. Rats were transfected with lenti-pre-AMO- miR-195 , lenti-pre-AMO -miR-195 + lenti-pre- miR-195 , or NC. Data shown were from six rats for each group. * p

    Journal: The Journal of Neuroscience

    Article Title: MicroRNA-195 Protects Against Dementia Induced by Chronic Brain Hypoperfusion via Its Anti-Amyloidogenic Effect in Rats

    doi: 10.1523/JNEUROSCI.1997-12.2013

    Figure Lengend Snippet: miR-195 knockdown produces learning and memory deficits in rats. A , Verification of the injection sites by Evans blue staining in stereotaxic surgery. B , Quantification of miR-195 in the hippocampus and the cortex tissues after stereotaxic injection for 8 weeks using qRT-PCR. Rats were transfected with lenti-pre-AMO- miR-195 , lenti-pre-AMO -miR-195 + lenti-pre- miR-195 , or NC. Data shown were from six rats for each group. * p

    Article Snippet: The TaqMan qRT-PCR probes and primers for miR-195 (catalog #4427975, ID: 000494), BACE1 (catalog #4331182; ID: Rn00569988_m1), APP (catalog #448892; ID: Rn00570673_ml), U6 (catalog #4427975; ID: 001973), and β-actin (catalog #4331182, ID: Rn00667869_ml) mRNA levels were designed by Applied Biosystems. qPCR was performed on a thermocycler ABI Prism 7500 fast (Applied Biosystems), and the protocol was as follows: (1) 95°C, 10 min; (2) 95°C, 15 s; (3) 60°C, and 1 min (repeat (2) and (3) for 40 cycles).

    Techniques: Injection, Staining, Quantitative RT-PCR, Transfection