qrt pcr analysis Bio Rad Search Results


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  • 90
    Thermo Fisher 7500 fast real time pcr system
    7500 Fast Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 32786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad quantitative real time polymerase chain reaction qrt pcr analysis
    Validation of the DGE analysis by quantitative real-time polymerase chain reaction <t>(qRT-PCR).</t> Error bars represent the standard deviations of qRT-PCR signals (n = 3).
    Quantitative Real Time Polymerase Chain Reaction Qrt Pcr Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad qrt pcr analysis
    PMK-1 Regulates Basal and Inducible Expression of P. aeruginosa –Induced Genes (A) Venn diagram of overlap between genes regulated by PMK-1 and P. aeruginosa. (B) (C) <t>qRT-PCR</t> analysis of PA14-induced gene expression in wild-type animals and in pmk-1 mutants. Results are the average of two biological replicates, each replicate measured in duplicate and normalized to a control gene. Error bars are SEM. (D) Diagram of different gene classes regulated by PMK-1 and/or P. aeruginosa. PMK-1 is required for basal and inducible regulations of class A genes. PMK-1 is required for basal, but not inducible expression of class B genes. PMK-1 is required for inducible but not basal expression of class C genes. PMK-1 is required for neither basal nor inducible expression of class D genes. PMK-1 regulates basal expression of class E genes, but these genes are not induced by P. aeruginosa.
    Qrt Pcr Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad qrt pcr bio rad
    TOR kinase phosphorylates and activates E2Fa a , Ectopic E2Fa activation of S-phase genes requires glucose-TOR signalling in leaf cells. WT or tor protoplasts expressing E2Fa-HA or S6K1-FLAG were treated without or with rapamycin (Rap) or antimycin A (AMA). <t>QRT-PCR</t> analyses. P-T449 indicates endogenous TOR kinase activity. Protein blot analysis (inset). b-c , TOR kinase directly phosphorylates E2Fa and 4E-BP1. Torin1 specifically inhibits TOR kinase. Staurosporine (Stau) inhibits S6K1 kinase. d , TOR directly interacts with E2Fa by immunoprecipitation (IP) and Western (W) blot analysis.
    Qrt Pcr Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad opticon qrt pcr analysis system
    TOR kinase phosphorylates and activates E2Fa a , Ectopic E2Fa activation of S-phase genes requires glucose-TOR signalling in leaf cells. WT or tor protoplasts expressing E2Fa-HA or S6K1-FLAG were treated without or with rapamycin (Rap) or antimycin A (AMA). <t>QRT-PCR</t> analyses. P-T449 indicates endogenous TOR kinase activity. Protein blot analysis (inset). b-c , TOR kinase directly phosphorylates E2Fa and 4E-BP1. Torin1 specifically inhibits TOR kinase. Staurosporine (Stau) inhibits S6K1 kinase. d , TOR directly interacts with E2Fa by immunoprecipitation (IP) and Western (W) blot analysis.
    Opticon Qrt Pcr Analysis System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 76/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad qrt pcr reaction
    TOR kinase phosphorylates and activates E2Fa a , Ectopic E2Fa activation of S-phase genes requires glucose-TOR signalling in leaf cells. WT or tor protoplasts expressing E2Fa-HA or S6K1-FLAG were treated without or with rapamycin (Rap) or antimycin A (AMA). <t>QRT-PCR</t> analyses. P-T449 indicates endogenous TOR kinase activity. Protein blot analysis (inset). b-c , TOR kinase directly phosphorylates E2Fa and 4E-BP1. Torin1 specifically inhibits TOR kinase. Staurosporine (Stau) inhibits S6K1 kinase. d , TOR directly interacts with E2Fa by immunoprecipitation (IP) and Western (W) blot analysis.
    Qrt Pcr Reaction, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad quantitative qrt pcr analyses
    <t>qRT-PCR</t> analysis was performed at 1-, 3-, and 7-h post-laser treatment of the laser spot and the adjacent tissue region compared to control for the eleven up-regulated genes of interest. In addition to the genes of interest, qRT-PCR on luciferase for
    Quantitative Qrt Pcr Analyses, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 78/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad sybr green qrt pcr
    Replication kinetics of influenza virus assessed by <t>qRT-PCR.</t> Virus replication was assessed in the absence or presence of TPCK-trypsin (1 µg/mL). Virus (1000 TCID 50 in 100 µL) was placed into a well of a 96-well plate. After incubation for 1 h at 37°C, a suspension of MDCK-London cells (30,000 in 100 µL) was added. At the indicated times, experimental samples were prepared using SPR and subjected to qRT-PCR. The RNA copy numbers were normalized to the mean value observed for A/PR/8/34 at 6 hours in the presence of TPCK-trypsin. Each point represents the mean ± SEM (n = 3).
    Sybr Green Qrt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher downstream qrt pcr analysis
    Replication kinetics of influenza virus assessed by <t>qRT-PCR.</t> Virus replication was assessed in the absence or presence of TPCK-trypsin (1 µg/mL). Virus (1000 TCID 50 in 100 µL) was placed into a well of a 96-well plate. After incubation for 1 h at 37°C, a suspension of MDCK-London cells (30,000 in 100 µL) was added. At the indicated times, experimental samples were prepared using SPR and subjected to qRT-PCR. The RNA copy numbers were normalized to the mean value observed for A/PR/8/34 at 6 hours in the presence of TPCK-trypsin. Each point represents the mean ± SEM (n = 3).
    Downstream Qrt Pcr Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad reverse transcription pcr qrt pcr
    Replication kinetics of influenza virus assessed by <t>qRT-PCR.</t> Virus replication was assessed in the absence or presence of TPCK-trypsin (1 µg/mL). Virus (1000 TCID 50 in 100 µL) was placed into a well of a 96-well plate. After incubation for 1 h at 37°C, a suspension of MDCK-London cells (30,000 in 100 µL) was added. At the indicated times, experimental samples were prepared using SPR and subjected to qRT-PCR. The RNA copy numbers were normalized to the mean value observed for A/PR/8/34 at 6 hours in the presence of TPCK-trypsin. Each point represents the mean ± SEM (n = 3).
    Reverse Transcription Pcr Qrt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad qrt pcr expression analysis
    Replication kinetics of influenza virus assessed by <t>qRT-PCR.</t> Virus replication was assessed in the absence or presence of TPCK-trypsin (1 µg/mL). Virus (1000 TCID 50 in 100 µL) was placed into a well of a 96-well plate. After incubation for 1 h at 37°C, a suspension of MDCK-London cells (30,000 in 100 µL) was added. At the indicated times, experimental samples were prepared using SPR and subjected to qRT-PCR. The RNA copy numbers were normalized to the mean value observed for A/PR/8/34 at 6 hours in the presence of TPCK-trypsin. Each point represents the mean ± SEM (n = 3).
    Qrt Pcr Expression Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 84/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad real time qrt pcr
    Relative expression of strawberry genes ( FaPE1 , FaPLA , FaPG1 , FaPG2 , FaOMT , FaQR , and FaGalUR ), as determined by <t>qRT–PCR,</t> in the achenes and receptacles of ripe strawberry fruits. Asterisks indicate significant differences between the transgenic lines and the control for each sample using ANOVA and the Tukey HSD test adjusted to a 95% significance level. Different letters indicate significant differences within the control lines (achene and receptacle) for each gene using ANOVA and the Tukey HSD test adjusted to a 95% significance level.
    Real Time Qrt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad opticon monitor qrt pcr detection system
    Relative expression of strawberry genes ( FaPE1 , FaPLA , FaPG1 , FaPG2 , FaOMT , FaQR , and FaGalUR ), as determined by <t>qRT–PCR,</t> in the achenes and receptacles of ripe strawberry fruits. Asterisks indicate significant differences between the transgenic lines and the control for each sample using ANOVA and the Tukey HSD test adjusted to a 95% significance level. Different letters indicate significant differences within the control lines (achene and receptacle) for each gene using ANOVA and the Tukey HSD test adjusted to a 95% significance level.
    Opticon Monitor Qrt Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad real time qrt pcr analysis
    Expression analysis of five genes in cotton anthers by <t>qRT-PCR.</t> The housekeeping gene 18S was used as an internal control; H276A, CMS line; H276B, maintainer line; and H276A/H268, F1 plants from the descendant of restorer H268 hybridized with H276A. Error bars represent standard deviation (n = 3). Double asterisk represents significance at 1% probability
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    Bio-Rad ssoadvanced qrt pcr universal probes supermix
    Expression analysis of five genes in cotton anthers by <t>qRT-PCR.</t> The housekeeping gene 18S was used as an internal control; H276A, CMS line; H276B, maintainer line; and H276A/H268, F1 plants from the descendant of restorer H268 hybridized with H276A. Error bars represent standard deviation (n = 3). Double asterisk represents significance at 1% probability
    Ssoadvanced Qrt Pcr Universal Probes Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 80/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad real time reverse transcription pcr qrt pcr
    Generation and analysis of mice expressing A-USF. (A) DNA construct pITRp543f2A-USF4 used to generate transgenic mice expressing dominant-negative USF (A-USF). The A-USF coding region is under the control of the human β-globin gene promoter (β-P) and 3′ enhancer (3′E) as well as human LCR elements HS2 and HS3 plus flanking DNA. The DNA construct is flanked on either site by insulator elements derived from the chicken β-globin gene locus (cHS4). (B) SYBR green stain of the <t>RT-PCR</t> analysis of A-USF expression in transgenic (founders I and III and line II F 1 littermates 1 to 3 [II/1 to II/3]) and wild-type (WT) mice. RNA was isolated from the spleens of phenylhydrazine-treated mice, reverse transcribed, subjected to PCR analysis with primers specific to the A-USF coding region, and electrophoresed in 5% Tris-borate-EDTA polyacrylamide gels. (C) Western blot analysis of A-USF expression in transgenic or wild-type mice. Protein was isolated from the spleen or liver of phenylhydrazine-treated mice and subjected to Western blot analysis using an antibody against USF1, which also detects A-USF. (D) <t>qRT-PCR</t> analysis of β maj -globin gene expression in spleens of A-USF transgenic line II F 1 littermates, A-USF founder mouse I, and wild-type mice. Data from the three line II F 1 littermates were combined and are designated II/1/2/3. GAPDH was used as a loading control, and results from samples were normalized to those of the wild type. (E) ChIP analysis of RNA Pol II and USF2 interactions with the β maj -globin gene promoter control mice (WT) and transgenic mice (A-USF). Spleens taken from two phenylhydrazine-treated F 1 females (derived from line II) or wild-type mice were homogenized and subjected to ChIP analysis using antibodies against IgG, RNA Pol II, or USF2. Error bars reflect standard deviations from two independent experiments.
    Real Time Reverse Transcription Pcr Qrt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 83/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad qrt pcr data opticon monitor 3 software
    Generation and analysis of mice expressing A-USF. (A) DNA construct pITRp543f2A-USF4 used to generate transgenic mice expressing dominant-negative USF (A-USF). The A-USF coding region is under the control of the human β-globin gene promoter (β-P) and 3′ enhancer (3′E) as well as human LCR elements HS2 and HS3 plus flanking DNA. The DNA construct is flanked on either site by insulator elements derived from the chicken β-globin gene locus (cHS4). (B) SYBR green stain of the <t>RT-PCR</t> analysis of A-USF expression in transgenic (founders I and III and line II F 1 littermates 1 to 3 [II/1 to II/3]) and wild-type (WT) mice. RNA was isolated from the spleens of phenylhydrazine-treated mice, reverse transcribed, subjected to PCR analysis with primers specific to the A-USF coding region, and electrophoresed in 5% Tris-borate-EDTA polyacrylamide gels. (C) Western blot analysis of A-USF expression in transgenic or wild-type mice. Protein was isolated from the spleen or liver of phenylhydrazine-treated mice and subjected to Western blot analysis using an antibody against USF1, which also detects A-USF. (D) <t>qRT-PCR</t> analysis of β maj -globin gene expression in spleens of A-USF transgenic line II F 1 littermates, A-USF founder mouse I, and wild-type mice. Data from the three line II F 1 littermates were combined and are designated II/1/2/3. GAPDH was used as a loading control, and results from samples were normalized to those of the wild type. (E) ChIP analysis of RNA Pol II and USF2 interactions with the β maj -globin gene promoter control mice (WT) and transgenic mice (A-USF). Spleens taken from two phenylhydrazine-treated F 1 females (derived from line II) or wild-type mice were homogenized and subjected to ChIP analysis using antibodies against IgG, RNA Pol II, or USF2. Error bars reflect standard deviations from two independent experiments.
    Qrt Pcr Data Opticon Monitor 3 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad sybr green real time qrt pcr analysis real time qrt pcr
    Hepatopancreatic Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time <t>qRT-PCR</t> . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P
    Sybr Green Real Time Qrt Pcr Analysis Real Time Qrt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad quantitative rt pcr qrt pcr analysis qrt pcrs
    Hepatopancreatic Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time <t>qRT-PCR</t> . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P
    Quantitative Rt Pcr Qrt Pcr Analysis Qrt Pcrs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad quantitative real time pcr qrt pcr quantitative real time pcr
    Hepatopancreatic Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time <t>qRT-PCR</t> . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P
    Quantitative Real Time Pcr Qrt Pcr Quantitative Real Time Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 79/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad real time pcr system
    Hepatopancreatic Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time <t>qRT-PCR</t> . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P
    Real Time Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 3435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hepatopancreatic Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time <t>qRT-PCR</t> . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P
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    Hepatopancreatic Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time <t>qRT-PCR</t> . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P
    Cfx Connect Real Time Pcr System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad iq5 real time pcr detection system
    Hepatopancreatic Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time <t>qRT-PCR</t> . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P
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    Bio-Rad cfx connecttm optics module
    Hepatopancreatic Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time <t>qRT-PCR</t> . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P
    Cfx Connecttm Optics Module, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Validation of the DGE analysis by quantitative real-time polymerase chain reaction (qRT-PCR). Error bars represent the standard deviations of qRT-PCR signals (n = 3).

    Journal: PLoS ONE

    Article Title: RNA-seq approach to analysis of gene expression profiles in dark green islands and light green tissues of Cucumber mosaic virus-infected Nicotiana tabacum

    doi: 10.1371/journal.pone.0175391

    Figure Lengend Snippet: Validation of the DGE analysis by quantitative real-time polymerase chain reaction (qRT-PCR). Error bars represent the standard deviations of qRT-PCR signals (n = 3).

    Article Snippet: Quantitative real-time polymerase chain reaction validation Validation of the RNA-seq data for 12 different genes was performed using quantitative real-time polymerase chain reaction (qRT-PCR) analysis (Bio-Rad Laboratories, Hercules, CA, USA).

    Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    PMK-1 Regulates Basal and Inducible Expression of P. aeruginosa –Induced Genes (A) Venn diagram of overlap between genes regulated by PMK-1 and P. aeruginosa. (B) (C) qRT-PCR analysis of PA14-induced gene expression in wild-type animals and in pmk-1 mutants. Results are the average of two biological replicates, each replicate measured in duplicate and normalized to a control gene. Error bars are SEM. (D) Diagram of different gene classes regulated by PMK-1 and/or P. aeruginosa. PMK-1 is required for basal and inducible regulations of class A genes. PMK-1 is required for basal, but not inducible expression of class B genes. PMK-1 is required for inducible but not basal expression of class C genes. PMK-1 is required for neither basal nor inducible expression of class D genes. PMK-1 regulates basal expression of class E genes, but these genes are not induced by P. aeruginosa.

    Journal: PLoS Genetics

    Article Title: p38 MAPK Regulates Expression of Immune Response Genes and Contributes to Longevity in C. elegans

    doi: 10.1371/journal.pgen.0020183

    Figure Lengend Snippet: PMK-1 Regulates Basal and Inducible Expression of P. aeruginosa –Induced Genes (A) Venn diagram of overlap between genes regulated by PMK-1 and P. aeruginosa. (B) (C) qRT-PCR analysis of PA14-induced gene expression in wild-type animals and in pmk-1 mutants. Results are the average of two biological replicates, each replicate measured in duplicate and normalized to a control gene. Error bars are SEM. (D) Diagram of different gene classes regulated by PMK-1 and/or P. aeruginosa. PMK-1 is required for basal and inducible regulations of class A genes. PMK-1 is required for basal, but not inducible expression of class B genes. PMK-1 is required for inducible but not basal expression of class C genes. PMK-1 is required for neither basal nor inducible expression of class D genes. PMK-1 regulates basal expression of class E genes, but these genes are not induced by P. aeruginosa.

    Article Snippet: This cDNA was then subjected to qRT-PCR analysis using SYBR green detection on an iCycler machine (Bio-Rad, http://www.bio-rad.com ).

    Techniques: Expressing, Quantitative RT-PCR

    Validation of microarray data by qRT-PCR. To validate the results of the microarray data, we selected 20 F. graminearum genes showing strong expression ratios by microarray analysis. Due to the different expression intensities among genes, we divided the 20 genes into three groups for better visualization of qRT-PCR results: expression intensities less than 4 ( A ), between 4 and 100 ( B ), and more than 100 ( C ). Abbreviations: VF, virus-free; VI, virus-infected. The number in the table indicates the expression intensity of each gene.

    Journal: BMC Genomics

    Article Title: Genome-wide expression profiling shows transcriptional reprogramming in Fusarium graminearum by Fusarium graminearum virus 1-DK21 infection

    doi: 10.1186/1471-2164-13-173

    Figure Lengend Snippet: Validation of microarray data by qRT-PCR. To validate the results of the microarray data, we selected 20 F. graminearum genes showing strong expression ratios by microarray analysis. Due to the different expression intensities among genes, we divided the 20 genes into three groups for better visualization of qRT-PCR results: expression intensities less than 4 ( A ), between 4 and 100 ( B ), and more than 100 ( C ). Abbreviations: VF, virus-free; VI, virus-infected. The number in the table indicates the expression intensity of each gene.

    Article Snippet: qRT-PCR analysis qRT-PCR was performed on Bio-Rad’s CFX96™ Real-time PCR system using gene-specific internal primers.

    Techniques: Microarray, Quantitative RT-PCR, Expressing, Infection

    qRT-PCR analysis of CTA expression in cohort 1 Data are expressed as means calculated out of assays run in triplicate. β-actin was used as internal reference to compute the δCt values.

    Journal: Oncotarget

    Article Title: Novel antigens in non-small cell lung cancer: SP17, AKAP4, and PTTG1 are potential immunotherapeutic targets

    doi:

    Figure Lengend Snippet: qRT-PCR analysis of CTA expression in cohort 1 Data are expressed as means calculated out of assays run in triplicate. β-actin was used as internal reference to compute the δCt values.

    Article Snippet: qRT-PCR qRT-PCR analysis was performed as previously described[ ], using an iCycler iQ Real Time PCR machine SYBR ® Green I Supermix (both from Bio-Rad Laboratories, Inc).

    Techniques: Quantitative RT-PCR, Expressing

    miR-33b expression is induced during human SGBS preadipocyte differentiation. (A) Representative phase-contrast images of undifferentiated and differentiated SGBS cells at different time points. (B to D) qRT-PCR analysis of PPAR γ (B), GLUT4 (C), and adiponectin gene (D) expression in undifferentiated and differentiated SGBS cells at different time points. (E) qRT-PCR analysis of SREBP-1a , SREBP-1c , and SREBP-2 expression in undifferentiated and differentiated SGBS cells at different time points. (F) qRT-PCR analysis of miR-33a and miR-33b expression in undifferentiated and differentiated SGBS cells at different time points. qRT-PCR data are expressed as means and SEM.

    Journal: Molecular and Cellular Biology

    Article Title: SREBP-1c/MicroRNA 33b Genomic Loci Control Adipocyte Differentiation

    doi: 10.1128/MCB.00745-15

    Figure Lengend Snippet: miR-33b expression is induced during human SGBS preadipocyte differentiation. (A) Representative phase-contrast images of undifferentiated and differentiated SGBS cells at different time points. (B to D) qRT-PCR analysis of PPAR γ (B), GLUT4 (C), and adiponectin gene (D) expression in undifferentiated and differentiated SGBS cells at different time points. (E) qRT-PCR analysis of SREBP-1a , SREBP-1c , and SREBP-2 expression in undifferentiated and differentiated SGBS cells at different time points. (F) qRT-PCR analysis of miR-33a and miR-33b expression in undifferentiated and differentiated SGBS cells at different time points. qRT-PCR data are expressed as means and SEM.

    Article Snippet: Quantitative real-time (qRT)-PCR analysis was performed in duplicate using SsoFast EvaGreen Supermix (Bio-Rad) on an iCycler real-time detection system (Eppendorf).

    Techniques: Expressing, Quantitative RT-PCR

    miR-33b overexpression impairs adipocyte differentiation, while miR-33b inhibition promotes lipid droplet accumulation. (A) Representative phase-contrast, GFP, and Oil Red O images of SGBS cells transduced with control, pre-miR-33b, or anti-miR-33b lentivirus and differentiated for 15 days. (B) Spectrophotometric quantification analysis of Oil Red O staining in SGBS cells transduced with control, pre-miR-33b, or anti-miR-33b lentivirus and differentiated for 8 or 15 days. RFU, relative fluorescence units. (C) Triglyceride quantification in SGBS cells transduced with control, pre-miR-33b, or anti-miR-33b lentivirus and differentiated for 0, 8, or 15 days. (D to G) qRT-PCR analysis of SREBP-1c (D), PPAR γ (E), GLUT4 (F), and adiponectin gene (G) expression in SGBS cells transduced with control, pre-miR-33b, or anti-miR-33b virus and differentiated for 0, 8, or 15 days. (H) qRT-PCR analysis of miR-33a and miR-33b in SGBS cells transduced with control or anti-miR-33b lentivirus. The data are expressed as means and SEM. *, P ≤ 0.05 versus cells transduced with control virus.

    Journal: Molecular and Cellular Biology

    Article Title: SREBP-1c/MicroRNA 33b Genomic Loci Control Adipocyte Differentiation

    doi: 10.1128/MCB.00745-15

    Figure Lengend Snippet: miR-33b overexpression impairs adipocyte differentiation, while miR-33b inhibition promotes lipid droplet accumulation. (A) Representative phase-contrast, GFP, and Oil Red O images of SGBS cells transduced with control, pre-miR-33b, or anti-miR-33b lentivirus and differentiated for 15 days. (B) Spectrophotometric quantification analysis of Oil Red O staining in SGBS cells transduced with control, pre-miR-33b, or anti-miR-33b lentivirus and differentiated for 8 or 15 days. RFU, relative fluorescence units. (C) Triglyceride quantification in SGBS cells transduced with control, pre-miR-33b, or anti-miR-33b lentivirus and differentiated for 0, 8, or 15 days. (D to G) qRT-PCR analysis of SREBP-1c (D), PPAR γ (E), GLUT4 (F), and adiponectin gene (G) expression in SGBS cells transduced with control, pre-miR-33b, or anti-miR-33b virus and differentiated for 0, 8, or 15 days. (H) qRT-PCR analysis of miR-33a and miR-33b in SGBS cells transduced with control or anti-miR-33b lentivirus. The data are expressed as means and SEM. *, P ≤ 0.05 versus cells transduced with control virus.

    Article Snippet: Quantitative real-time (qRT)-PCR analysis was performed in duplicate using SsoFast EvaGreen Supermix (Bio-Rad) on an iCycler real-time detection system (Eppendorf).

    Techniques: Over Expression, Inhibition, Transduction, Staining, Fluorescence, Quantitative RT-PCR, Expressing

    Knockdown of HMGA2 impairs SGBS cell proliferation and differentiation. (A) qRT-PCR analysis of HMGA2 expression in SGBS preadipocytes transfected with nonsilencing siRNA, miR-33b mimics, or HMGA2 siRNA. (B) Cell number analysis of SGBS preadipocytes transfected with nonsilencing siRNA, miR-33b mimics, or HMGA2 siRNA at different time points. (C) qRT-PCR analysis of HMGA2 expression in undifferentiated and differentiated SGBS preadipocytes transfected with nonsilencing siRNA, miR-33b mimics, or HMGA2 siRNA. (D) Representative phase-contrast images of unstained or Oil Red O-stained differentiated SGBS preadipocytes transfected with nonsilencing or HMGA2 siRNA. (E) Quantification of Oil Red O staining from panel D. (F to I) qRT-PCR analysis of SREBP-1c (F), PPAR γ (G), GLUT4 (H), and adiponectin gene (I) expression in SGBS cells transfected with nonsilencing or HMGA2 siRNA and differentiated for 10 days. The data are expressed as means and SEM. *, P ≤ 0.05 versus cells transfected with nonsilencing siRNA.

    Journal: Molecular and Cellular Biology

    Article Title: SREBP-1c/MicroRNA 33b Genomic Loci Control Adipocyte Differentiation

    doi: 10.1128/MCB.00745-15

    Figure Lengend Snippet: Knockdown of HMGA2 impairs SGBS cell proliferation and differentiation. (A) qRT-PCR analysis of HMGA2 expression in SGBS preadipocytes transfected with nonsilencing siRNA, miR-33b mimics, or HMGA2 siRNA. (B) Cell number analysis of SGBS preadipocytes transfected with nonsilencing siRNA, miR-33b mimics, or HMGA2 siRNA at different time points. (C) qRT-PCR analysis of HMGA2 expression in undifferentiated and differentiated SGBS preadipocytes transfected with nonsilencing siRNA, miR-33b mimics, or HMGA2 siRNA. (D) Representative phase-contrast images of unstained or Oil Red O-stained differentiated SGBS preadipocytes transfected with nonsilencing or HMGA2 siRNA. (E) Quantification of Oil Red O staining from panel D. (F to I) qRT-PCR analysis of SREBP-1c (F), PPAR γ (G), GLUT4 (H), and adiponectin gene (I) expression in SGBS cells transfected with nonsilencing or HMGA2 siRNA and differentiated for 10 days. The data are expressed as means and SEM. *, P ≤ 0.05 versus cells transfected with nonsilencing siRNA.

    Article Snippet: Quantitative real-time (qRT)-PCR analysis was performed in duplicate using SsoFast EvaGreen Supermix (Bio-Rad) on an iCycler real-time detection system (Eppendorf).

    Techniques: Quantitative RT-PCR, Expressing, Transfection, Staining

    miR-33b overexpression impairs SGBS preadipocyte proliferation and clonal expansion. (A) Cell number analysis of SGBS preadipocytes transfected with negative-control miRNA (CM) or miR-33b mimics (miR-33b) at different time points. (B) qRT-PCR analysis of CDK6 , Cyclin D1 , HMGA2 , and SREBP-1 expression in undifferentiated and differentiated SGBS preadipocytes at different time points. (C) Cell numbers of SGBS preadipocytes transfected with CM or miR-33b and differentiated for 2 or 4 days. (D and E), qRT-PCR analysis of CDK6 and HMGA2 expression in differentiating SGBS preadipocytes treated with CM or miR-33b. The data are expressed as means and SEM. *, P ≤ 0.05 versus cells transfected with CM.

    Journal: Molecular and Cellular Biology

    Article Title: SREBP-1c/MicroRNA 33b Genomic Loci Control Adipocyte Differentiation

    doi: 10.1128/MCB.00745-15

    Figure Lengend Snippet: miR-33b overexpression impairs SGBS preadipocyte proliferation and clonal expansion. (A) Cell number analysis of SGBS preadipocytes transfected with negative-control miRNA (CM) or miR-33b mimics (miR-33b) at different time points. (B) qRT-PCR analysis of CDK6 , Cyclin D1 , HMGA2 , and SREBP-1 expression in undifferentiated and differentiated SGBS preadipocytes at different time points. (C) Cell numbers of SGBS preadipocytes transfected with CM or miR-33b and differentiated for 2 or 4 days. (D and E), qRT-PCR analysis of CDK6 and HMGA2 expression in differentiating SGBS preadipocytes treated with CM or miR-33b. The data are expressed as means and SEM. *, P ≤ 0.05 versus cells transfected with CM.

    Article Snippet: Quantitative real-time (qRT)-PCR analysis was performed in duplicate using SsoFast EvaGreen Supermix (Bio-Rad) on an iCycler real-time detection system (Eppendorf).

    Techniques: Over Expression, Transfection, Negative Control, Quantitative RT-PCR, Expressing

    miR-33b expression is induced during differentiation of human primary preadipocytes. (A) Representative phase-contrast images of undifferentiated and differentiated human primary preadipocytes at different time points. (B to D) qRT-PCR analysis of PPAR γ (B), GLUT4 (C), and adiponectin gene ( AdipoQ ) (D) expression in undifferentiated (Undiff) and differentiated (Diff) human primary preadipocytes at different time points (d, days). (E) qRT-PCR analysis of SREBP-1a , SREBP-1c , and SREBP-2 expression in undifferentiated and differentiated human primary preadipocytes at different time points. (F) qRT-PCR analysis of miR-33a and miR-33b expression in undifferentiated and differentiated human primary preadipocytes at different time points. (G) qRT-PCR analysis of miR-33b expression in stromal vascular and mature adipocyte fractions of WAT from human patients. qRT-PCR data are expressed as means and SEM.

    Journal: Molecular and Cellular Biology

    Article Title: SREBP-1c/MicroRNA 33b Genomic Loci Control Adipocyte Differentiation

    doi: 10.1128/MCB.00745-15

    Figure Lengend Snippet: miR-33b expression is induced during differentiation of human primary preadipocytes. (A) Representative phase-contrast images of undifferentiated and differentiated human primary preadipocytes at different time points. (B to D) qRT-PCR analysis of PPAR γ (B), GLUT4 (C), and adiponectin gene ( AdipoQ ) (D) expression in undifferentiated (Undiff) and differentiated (Diff) human primary preadipocytes at different time points (d, days). (E) qRT-PCR analysis of SREBP-1a , SREBP-1c , and SREBP-2 expression in undifferentiated and differentiated human primary preadipocytes at different time points. (F) qRT-PCR analysis of miR-33a and miR-33b expression in undifferentiated and differentiated human primary preadipocytes at different time points. (G) qRT-PCR analysis of miR-33b expression in stromal vascular and mature adipocyte fractions of WAT from human patients. qRT-PCR data are expressed as means and SEM.

    Article Snippet: Quantitative real-time (qRT)-PCR analysis was performed in duplicate using SsoFast EvaGreen Supermix (Bio-Rad) on an iCycler real-time detection system (Eppendorf).

    Techniques: Expressing, Quantitative RT-PCR

    Regulation of HMGA2 in human preadipocytes and possibly patients with lipoma. (A and B) Western blot (A) and quantification (B) of HSP90 and HMGA2 in SGBS preadipocytes in untreated, undifferentiated cells or cells transduced with control, pre-miR-33b, or anti-miR-33b virus followed by 3 days of differentiation. *, P ≤ 0.05 versus cells transduced with control virus. (C) qRT-PCR analysis of HMGA2 expression in SGBS cells transduced with control or pre-miR-33b lentivirus at 0, 8, and 15 days of differentiation. *, P ≤ 0.05 versus cells transduced with control virus. (D) qRT-PCR analysis of HMGA2 expression in SGBS preadipocytes at different time points after differentiation. (E) Identification of miR-33 binding sites (boldface) in the HMGA2 3′ UTR. (F) Luciferase activity assays of HMGA2 3′ UTR with or without mutations in binding sites for miR-33. (G to I) qRT-PCR analysis of HMGA2 , miR-33a, and miR-33b expression in subcutaneous adipose tissue (SAT) or lipoma samples from human patients. *, P ≤ 0.05 versus SAT. All data are expressed as means and SEM.

    Journal: Molecular and Cellular Biology

    Article Title: SREBP-1c/MicroRNA 33b Genomic Loci Control Adipocyte Differentiation

    doi: 10.1128/MCB.00745-15

    Figure Lengend Snippet: Regulation of HMGA2 in human preadipocytes and possibly patients with lipoma. (A and B) Western blot (A) and quantification (B) of HSP90 and HMGA2 in SGBS preadipocytes in untreated, undifferentiated cells or cells transduced with control, pre-miR-33b, or anti-miR-33b virus followed by 3 days of differentiation. *, P ≤ 0.05 versus cells transduced with control virus. (C) qRT-PCR analysis of HMGA2 expression in SGBS cells transduced with control or pre-miR-33b lentivirus at 0, 8, and 15 days of differentiation. *, P ≤ 0.05 versus cells transduced with control virus. (D) qRT-PCR analysis of HMGA2 expression in SGBS preadipocytes at different time points after differentiation. (E) Identification of miR-33 binding sites (boldface) in the HMGA2 3′ UTR. (F) Luciferase activity assays of HMGA2 3′ UTR with or without mutations in binding sites for miR-33. (G to I) qRT-PCR analysis of HMGA2 , miR-33a, and miR-33b expression in subcutaneous adipose tissue (SAT) or lipoma samples from human patients. *, P ≤ 0.05 versus SAT. All data are expressed as means and SEM.

    Article Snippet: Quantitative real-time (qRT)-PCR analysis was performed in duplicate using SsoFast EvaGreen Supermix (Bio-Rad) on an iCycler real-time detection system (Eppendorf).

    Techniques: Western Blot, Transduction, Quantitative RT-PCR, Expressing, Binding Assay, Luciferase, Activity Assay

    Real-Time PCR validation of FSHD-1 microarray data. A: Table reports the genes analyzed in qRT-PCR with fold-change and p-value. The data obtained in the FSHD-1 myoblasts gene chip array and the biological processes identified by the DAVID program, are also reported. The analysis was performed on seven FSHD-1 and six CN myoblast cell lines. B) Bar diagrams show relative expression of PAX3, MYOD1, MYOG and MYH2 in control and FSHD-1 myoblasts (day 0) and myotubes (day 8) relative to GAPDH. *** p-value

    Journal: PLoS ONE

    Article Title: Expression Profiling of FSHD-1 and FSHD-2 Cells during Myogenic Differentiation Evidences Common and Distinctive Gene Dysregulation Patterns

    doi: 10.1371/journal.pone.0020966

    Figure Lengend Snippet: Real-Time PCR validation of FSHD-1 microarray data. A: Table reports the genes analyzed in qRT-PCR with fold-change and p-value. The data obtained in the FSHD-1 myoblasts gene chip array and the biological processes identified by the DAVID program, are also reported. The analysis was performed on seven FSHD-1 and six CN myoblast cell lines. B) Bar diagrams show relative expression of PAX3, MYOD1, MYOG and MYH2 in control and FSHD-1 myoblasts (day 0) and myotubes (day 8) relative to GAPDH. *** p-value

    Article Snippet: Quantitative RT-PCR (qRT-PCR) analysis was performed on an IQ™5 Multicolor Real-Time PCR Detection System (Biorad) by TaqMan® Gene Expression Assays (Applied Biosystem) , or SYBR Green (Biorad) ( ).

    Techniques: Real-time Polymerase Chain Reaction, Microarray, Quantitative RT-PCR, Chromatin Immunoprecipitation, Expressing

    qRT-PCR of four candidate DEGs in the roots of 14 rice varieties. (A,B) indicated two DEGs that differentially expressed between deep rooting and shallow rooting varieties; (C,D) indicated two DEGs that differentially expressed between deep roots and shallow roots from one variety.

    Journal: Frontiers in Plant Science

    Article Title: Root Transcriptomic Analysis Revealing the Importance of Energy Metabolism to the Development of Deep Roots in Rice (Oryza sativa L.)

    doi: 10.3389/fpls.2017.01314

    Figure Lengend Snippet: qRT-PCR of four candidate DEGs in the roots of 14 rice varieties. (A,B) indicated two DEGs that differentially expressed between deep rooting and shallow rooting varieties; (C,D) indicated two DEGs that differentially expressed between deep roots and shallow roots from one variety.

    Article Snippet: qRT-PCR and microarray confirmation Some DEGs were selected for quantitative confirmation by quantitative real-time PCR (qRT-PCR) analysis with a BIO–RAD CFX96 Thermal Cycler (Bio-Rad, USA) (Livak and Schmittgen, ).

    Techniques: Quantitative RT-PCR

    Schematic illustration of basic molecular characterization of odorant binding protein11 from Spoladea recurvalis ( a ) Nucleotide and deduced amino acid sequence of OBP11.The conserved cysteine residues are marked in square box. Predicted signal peptide was highlighted in underline. ( b ) qRT-PCR analysis shown the expression of Spre-OBP11 relative to each development stages (Inst-Instar; PMD-Pupae male day; PFD-Pupae female day; VMD-virgin male day; VFD-virgin female day; AMD-Mated adult male day; AFD-Mated adult female day). GAPDH was used as an internal control. Bar represents ±standard error. Different letters ( a , b , c ) shows the significant differences (P

    Journal: Scientific Reports

    Article Title: Sex-specific spatial and temporal gene expressions of Pheromone biosynthesis activating neuropeptide (PBAN) and binding proteins (PBP/OBP) in Spoladea recurvalis

    doi: 10.1038/s41598-019-39822-x

    Figure Lengend Snippet: Schematic illustration of basic molecular characterization of odorant binding protein11 from Spoladea recurvalis ( a ) Nucleotide and deduced amino acid sequence of OBP11.The conserved cysteine residues are marked in square box. Predicted signal peptide was highlighted in underline. ( b ) qRT-PCR analysis shown the expression of Spre-OBP11 relative to each development stages (Inst-Instar; PMD-Pupae male day; PFD-Pupae female day; VMD-virgin male day; VFD-virgin female day; AMD-Mated adult male day; AFD-Mated adult female day). GAPDH was used as an internal control. Bar represents ±standard error. Different letters ( a , b , c ) shows the significant differences (P

    Article Snippet: Quantitative real-time PCR (qRT-PCR) analysis Quantitative RT-PCR was carried out with a Bio-Rad CFX system in the final reaction volume of 10 μLcontaining 2.5 μL cDNA of nuclease-free water, 5x SYBER Green Real-time PCR Master Mix and 10 μM each of forward and reverse primers (Supplementary Table.).

    Techniques: Binding Assay, Sequencing, Quantitative RT-PCR, Expressing

    Schematic illustration of basic molecular characterization of odorant binding protein4 (OBP4) from Spoladea recurvalis . ( a ) cDNA and derived amino acid sequence of Spre-OBP4.The conserved cysteine residues are marked in square box. Predicted signal peptide was highlighted in underline. ( b ) qRT-PCR analysis shown the expression of Spre-GOBP1 relative to each development stages (Inst-Instar; PMD-Pupae male day; PFD-Pupae female day; VMD-virgin male day; VFD-virgin female day; AMD-Mated adult male day; AFD-Mated adult female day). GAPDH was used as an internal control. Bar represents ±standard error. Different letters ( a , b , c ) shows the significant differences (P

    Journal: Scientific Reports

    Article Title: Sex-specific spatial and temporal gene expressions of Pheromone biosynthesis activating neuropeptide (PBAN) and binding proteins (PBP/OBP) in Spoladea recurvalis

    doi: 10.1038/s41598-019-39822-x

    Figure Lengend Snippet: Schematic illustration of basic molecular characterization of odorant binding protein4 (OBP4) from Spoladea recurvalis . ( a ) cDNA and derived amino acid sequence of Spre-OBP4.The conserved cysteine residues are marked in square box. Predicted signal peptide was highlighted in underline. ( b ) qRT-PCR analysis shown the expression of Spre-GOBP1 relative to each development stages (Inst-Instar; PMD-Pupae male day; PFD-Pupae female day; VMD-virgin male day; VFD-virgin female day; AMD-Mated adult male day; AFD-Mated adult female day). GAPDH was used as an internal control. Bar represents ±standard error. Different letters ( a , b , c ) shows the significant differences (P

    Article Snippet: Quantitative real-time PCR (qRT-PCR) analysis Quantitative RT-PCR was carried out with a Bio-Rad CFX system in the final reaction volume of 10 μLcontaining 2.5 μL cDNA of nuclease-free water, 5x SYBER Green Real-time PCR Master Mix and 10 μM each of forward and reverse primers (Supplementary Table.).

    Techniques: Binding Assay, Derivative Assay, Sequencing, Quantitative RT-PCR, Expressing

    Phylogenetic analysis and Gene expression of PBAN during different developmental stages. ( a ) Maximum likelihood phylogeny tree was constructed using known and putative PBAN amino acid sequences inferred using four insect orders determined by using MEGA 6.0 with default settings model JTT + T inferred from 1000 replicates. ( b ) PBAN gene expression in the development of S. recurvalis . Total RNA was prepared at day 1, day 3 and day 5 of each stages Larvae, Pupae, Virgin (male and female), Adult (mated male and female) subjected to qRT-PCR. The results are shown as the Spre-PBAN gene expression level was normalized related to each development stages. GAPDH was used as internal control. Bar represents the ±standard error. Different letters ( a , b , c ) shows the significant differences (P

    Journal: Scientific Reports

    Article Title: Sex-specific spatial and temporal gene expressions of Pheromone biosynthesis activating neuropeptide (PBAN) and binding proteins (PBP/OBP) in Spoladea recurvalis

    doi: 10.1038/s41598-019-39822-x

    Figure Lengend Snippet: Phylogenetic analysis and Gene expression of PBAN during different developmental stages. ( a ) Maximum likelihood phylogeny tree was constructed using known and putative PBAN amino acid sequences inferred using four insect orders determined by using MEGA 6.0 with default settings model JTT + T inferred from 1000 replicates. ( b ) PBAN gene expression in the development of S. recurvalis . Total RNA was prepared at day 1, day 3 and day 5 of each stages Larvae, Pupae, Virgin (male and female), Adult (mated male and female) subjected to qRT-PCR. The results are shown as the Spre-PBAN gene expression level was normalized related to each development stages. GAPDH was used as internal control. Bar represents the ±standard error. Different letters ( a , b , c ) shows the significant differences (P

    Article Snippet: Quantitative real-time PCR (qRT-PCR) analysis Quantitative RT-PCR was carried out with a Bio-Rad CFX system in the final reaction volume of 10 μLcontaining 2.5 μL cDNA of nuclease-free water, 5x SYBER Green Real-time PCR Master Mix and 10 μM each of forward and reverse primers (Supplementary Table.).

    Techniques: Expressing, Construct, Quantitative RT-PCR

    Schematic illustration of basic molecular characterization of general odorant binding protein1 (GOBP1) from Spoladea recurvalis ( a ) cDNA and derived amino acid sequence of Spre-GOBP1.The conserved cysteine residues are marked in square box. Predicted signal peptide was highlighted in underline. ( b ) qRT-PCR analysis shown the expression of Spre-GOBP1 relative to each development stages.(Inst-Instar; PMD-Pupae male day; PFD-Pupae female day; VMD-virgin male day; VFD-virgin female day; AMD-Mated adult male day; AFD-Mated adult female day).GAPDH was used as an internal control. Bar represents ±standard error. Different letters ( a , b , c ) shows the significant differences (P

    Journal: Scientific Reports

    Article Title: Sex-specific spatial and temporal gene expressions of Pheromone biosynthesis activating neuropeptide (PBAN) and binding proteins (PBP/OBP) in Spoladea recurvalis

    doi: 10.1038/s41598-019-39822-x

    Figure Lengend Snippet: Schematic illustration of basic molecular characterization of general odorant binding protein1 (GOBP1) from Spoladea recurvalis ( a ) cDNA and derived amino acid sequence of Spre-GOBP1.The conserved cysteine residues are marked in square box. Predicted signal peptide was highlighted in underline. ( b ) qRT-PCR analysis shown the expression of Spre-GOBP1 relative to each development stages.(Inst-Instar; PMD-Pupae male day; PFD-Pupae female day; VMD-virgin male day; VFD-virgin female day; AMD-Mated adult male day; AFD-Mated adult female day).GAPDH was used as an internal control. Bar represents ±standard error. Different letters ( a , b , c ) shows the significant differences (P

    Article Snippet: Quantitative real-time PCR (qRT-PCR) analysis Quantitative RT-PCR was carried out with a Bio-Rad CFX system in the final reaction volume of 10 μLcontaining 2.5 μL cDNA of nuclease-free water, 5x SYBER Green Real-time PCR Master Mix and 10 μM each of forward and reverse primers (Supplementary Table.).

    Techniques: Binding Assay, Derivative Assay, Sequencing, Quantitative RT-PCR, Expressing

    Schematic illustration of basic molecular characterization of pheromone binding protein (PBP) from Spoladea recurvalis . ( a ) 5′ missing partial cDNA and derived amino acid sequence of Spre-PBP. The conserved cysteine residues are marked in square box. ( b ) qRT-PCR analysis shown the expression of Spre-PBP relative to each development stages.(Inst-Instar; PMD-Pupae male day; PFD-Pupae female day; VMD-virgin male day; VFD-virgin female day; AMD-Mated adult male day; AFD-Mated adult female day). GAPDH was used as an internal control. Bar represents ±standard error. Different letters ( a , b , c ) shows the significant differences (P

    Journal: Scientific Reports

    Article Title: Sex-specific spatial and temporal gene expressions of Pheromone biosynthesis activating neuropeptide (PBAN) and binding proteins (PBP/OBP) in Spoladea recurvalis

    doi: 10.1038/s41598-019-39822-x

    Figure Lengend Snippet: Schematic illustration of basic molecular characterization of pheromone binding protein (PBP) from Spoladea recurvalis . ( a ) 5′ missing partial cDNA and derived amino acid sequence of Spre-PBP. The conserved cysteine residues are marked in square box. ( b ) qRT-PCR analysis shown the expression of Spre-PBP relative to each development stages.(Inst-Instar; PMD-Pupae male day; PFD-Pupae female day; VMD-virgin male day; VFD-virgin female day; AMD-Mated adult male day; AFD-Mated adult female day). GAPDH was used as an internal control. Bar represents ±standard error. Different letters ( a , b , c ) shows the significant differences (P

    Article Snippet: Quantitative real-time PCR (qRT-PCR) analysis Quantitative RT-PCR was carried out with a Bio-Rad CFX system in the final reaction volume of 10 μLcontaining 2.5 μL cDNA of nuclease-free water, 5x SYBER Green Real-time PCR Master Mix and 10 μM each of forward and reverse primers (Supplementary Table.).

    Techniques: Binding Assay, Derivative Assay, Sequencing, Quantitative RT-PCR, Expressing

    Effects of NO and SP lipid extract incubation on hepatic expression of genes for cholesterol biosynthesis and fatty acid metabolism. HepG2 cells (A, B, C) and primary hepatocytes isolated (D, E) from C57BL/6J mice were separately incubated with 50 μg/mL concentration for 24 h and analyzed by qRT-PCR for transcriptional gene expression and western blot for protein expression. Data are expressed as relative expression to control, and bars with a different letter are significantly different ( P

    Journal: Journal of Medicinal Food

    Article Title: Hypolipidemic Effect of a Blue-Green Alga (Nostoc commune) Is Attributed to Its Nonlipid Fraction by Decreasing Intestinal Cholesterol Absorption in C57BL/6J Mice

    doi: 10.1089/jmf.2014.0121

    Figure Lengend Snippet: Effects of NO and SP lipid extract incubation on hepatic expression of genes for cholesterol biosynthesis and fatty acid metabolism. HepG2 cells (A, B, C) and primary hepatocytes isolated (D, E) from C57BL/6J mice were separately incubated with 50 μg/mL concentration for 24 h and analyzed by qRT-PCR for transcriptional gene expression and western blot for protein expression. Data are expressed as relative expression to control, and bars with a different letter are significantly different ( P

    Article Snippet: Total RNA was isolated from tissue samples using TRIzol reagent (Invitrogen, Grand Island, NY, USA), and quantitative real-time PCR (qRT-PCR) analysis for hepatic gene expression was conducted as previously described using the SYBR Green procedure and CFX96 real-time PCR detection system (BioRad, Hercules, CA, USA)., Primer sequences were designed according to GenBank database using the Beacon Designer software (Premier Biosoft, Palo Alto, CA, USA), and primer sequences are listed in .

    Techniques: Incubation, Expressing, Isolation, Mouse Assay, Concentration Assay, Quantitative RT-PCR, Western Blot

    Expression profiles of candidate genes. The expression profiles of selected genes were detected by qRT-PCR using the third instar larvae at different time intervals (0, 2, 4, 6, 12, 24 hr) after challenge by E. coli (A) or S. aureus (B), in which actin acted as the quantity and quality control to normalize interest gene expression level. The error bars represent the mean ± SD of three repeat amplifications. The asterisks represent significant differences from the control (unpaired t -test, *** P

    Journal: G3: Genes|Genomes|Genetics

    Article Title: Transcriptomic Analysis of Musca domestica to Reveal Key Genes of the Prophenoloxidase-Activating System

    doi: 10.1534/g3.115.016899

    Figure Lengend Snippet: Expression profiles of candidate genes. The expression profiles of selected genes were detected by qRT-PCR using the third instar larvae at different time intervals (0, 2, 4, 6, 12, 24 hr) after challenge by E. coli (A) or S. aureus (B), in which actin acted as the quantity and quality control to normalize interest gene expression level. The error bars represent the mean ± SD of three repeat amplifications. The asterisks represent significant differences from the control (unpaired t -test, *** P

    Article Snippet: Real-time RT-PCR analysis Real-time RT-PCR (qRT-PCR) was performed using iQSYBR Green Supermix (Bio-Rad) and CFX96 Real-Time System (Bio-Rad) to investigate the expression profiles of selected genes that might be related to the proPO system in M. domestica .

    Techniques: Expressing, Quantitative RT-PCR

    qRT-PCR validation of the differentially expressed miRNAs and target genes from high-throughput sequencing analyses in tuberous root development of radish. a qRT-PCR validation of 11 miRNAs from high-throughput small RNA sequencing analyses. b qRT-PCR validation of six target genes from degradome analyses

    Journal: 3 Biotech

    Article Title: Identification of miRNAs and their targets in regulating tuberous root development in radish using small RNA and degradome analyses

    doi: 10.1007/s13205-018-1330-z

    Figure Lengend Snippet: qRT-PCR validation of the differentially expressed miRNAs and target genes from high-throughput sequencing analyses in tuberous root development of radish. a qRT-PCR validation of 11 miRNAs from high-throughput small RNA sequencing analyses. b qRT-PCR validation of six target genes from degradome analyses

    Article Snippet: Real-time quantitative PCR (qRT-PCR) analysis was used to validate the expression patterns of miRNAs and target genes using an IQ5 Real-Time PCR System (BIO-RAD, Hercules, CA, USA).

    Techniques: Quantitative RT-PCR, Next-Generation Sequencing, High Throughput Screening Assay, RNA Sequencing Assay

    DNA demethylation of ROR2 , p14 , and p16 in DME-expressing cells is accompanied by gene reactivation. (A) Schematic diagram of analyzed genes. Each vertical bar represents a CpG dinucleotide. Position of ATG codon is indicated as a red rectangle. Green arrows show the location of pyrosequencing primers and yellow arrows the location of qMSP primers. (B) Methylation levels analyzed by bisulfite pyrosequencing; CpG sites are shown as bars filled with black to represent percentage methylation. (C) Methylation levels analyzed by qMSP (D) Gene expression levels analyzed by qRT-PCR. Analyses were performed in non-transfected DLD-1 cells and independent transfectants expressing WT DME (DME 2, DME 10, and DME 13), a catalytically inactive mutant version (mut 7 and mut 13) or cells transfected with the empty vector. Values are shown relative to those detected in non-transfected cells. Data are the mean ± SE of three independent experiments.

    Journal: Epigenetics

    Article Title: DNA methylation reprogramming of human cancer cells by expression of a plant 5-methylcytosine DNA glycosylase

    doi: 10.1080/15592294.2017.1414128

    Figure Lengend Snippet: DNA demethylation of ROR2 , p14 , and p16 in DME-expressing cells is accompanied by gene reactivation. (A) Schematic diagram of analyzed genes. Each vertical bar represents a CpG dinucleotide. Position of ATG codon is indicated as a red rectangle. Green arrows show the location of pyrosequencing primers and yellow arrows the location of qMSP primers. (B) Methylation levels analyzed by bisulfite pyrosequencing; CpG sites are shown as bars filled with black to represent percentage methylation. (C) Methylation levels analyzed by qMSP (D) Gene expression levels analyzed by qRT-PCR. Analyses were performed in non-transfected DLD-1 cells and independent transfectants expressing WT DME (DME 2, DME 10, and DME 13), a catalytically inactive mutant version (mut 7 and mut 13) or cells transfected with the empty vector. Values are shown relative to those detected in non-transfected cells. Data are the mean ± SE of three independent experiments.

    Article Snippet: Quantitative real time RT-PCR (qRT-PCR) analysis was performed in a CFX Connect™ Real-Time PCR Detection System (Biorad) by mixing cDNA (from 1 μg total RNA) with iQ™ SYBR Green Supermix (Biorad) and specific primers (Table S4).

    Techniques: Expressing, Methylation, Quantitative RT-PCR, Transfection, Mutagenesis, Plasmid Preparation

    DME expression on three loci displays different types of methylation changes in DLD-1 cells. (A) Methylation levels analyzed by bisulfite pyrosequencing; Each vertical bar represents a CpG dinucleotide, and position of ATG codon is indicated as a red rectangle; green arrows show the location of pyrosequencing primers. Analyzed CpG sites are shown as bars filled with black to represent percentage methylation. (B) Gene expression levels analyzed by qRT-PCR. Values are shown relative to those detected in non-transfected cells. Data are the mean ± SE of three independent experiments.

    Journal: Epigenetics

    Article Title: DNA methylation reprogramming of human cancer cells by expression of a plant 5-methylcytosine DNA glycosylase

    doi: 10.1080/15592294.2017.1414128

    Figure Lengend Snippet: DME expression on three loci displays different types of methylation changes in DLD-1 cells. (A) Methylation levels analyzed by bisulfite pyrosequencing; Each vertical bar represents a CpG dinucleotide, and position of ATG codon is indicated as a red rectangle; green arrows show the location of pyrosequencing primers. Analyzed CpG sites are shown as bars filled with black to represent percentage methylation. (B) Gene expression levels analyzed by qRT-PCR. Values are shown relative to those detected in non-transfected cells. Data are the mean ± SE of three independent experiments.

    Article Snippet: Quantitative real time RT-PCR (qRT-PCR) analysis was performed in a CFX Connect™ Real-Time PCR Detection System (Biorad) by mixing cDNA (from 1 μg total RNA) with iQ™ SYBR Green Supermix (Biorad) and specific primers (Table S4).

    Techniques: Expressing, Methylation, Quantitative RT-PCR, Transfection

    MnSR-BI expression tissue distribution as determined by real-time qRT-PCR. Values are shown as mean ± SD ( n = 3). Bars with different letters represent significant differences ( P

    Journal: International Journal of Genomics

    Article Title: Scavenger Receptor Class B, Type I, a CD36 Related Protein in Macrobrachium nipponense: Characterization, RNA Interference, and Expression Analysis with Different Dietary Lipid Sources

    doi: 10.1155/2016/6325927

    Figure Lengend Snippet: MnSR-BI expression tissue distribution as determined by real-time qRT-PCR. Values are shown as mean ± SD ( n = 3). Bars with different letters represent significant differences ( P

    Article Snippet: The SYBR Premix Ex Taq™ Kit (Takara) was used for real-time quantitative RT-PCR (qRT-PCR) analysis in a CFX96™ Real-Time System (Bio-Rad, USA).

    Techniques: Expressing, Quantitative RT-PCR

    Real-time quantitative PCR analysis of the MnSR-BI transcript expression in the hepatopancreas of M. nipponense injected with either MnSR-BI or GFP dsRNA. Samples were obtained 48 h (a) and 96 h (b) after dsRNA injection and analyzed by real-time qRT-PCR. Bars indicate mean ± SD ( n = 3). ∗ P

    Journal: International Journal of Genomics

    Article Title: Scavenger Receptor Class B, Type I, a CD36 Related Protein in Macrobrachium nipponense: Characterization, RNA Interference, and Expression Analysis with Different Dietary Lipid Sources

    doi: 10.1155/2016/6325927

    Figure Lengend Snippet: Real-time quantitative PCR analysis of the MnSR-BI transcript expression in the hepatopancreas of M. nipponense injected with either MnSR-BI or GFP dsRNA. Samples were obtained 48 h (a) and 96 h (b) after dsRNA injection and analyzed by real-time qRT-PCR. Bars indicate mean ± SD ( n = 3). ∗ P

    Article Snippet: The SYBR Premix Ex Taq™ Kit (Takara) was used for real-time quantitative RT-PCR (qRT-PCR) analysis in a CFX96™ Real-Time System (Bio-Rad, USA).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Injection, Quantitative RT-PCR

    TOR kinase phosphorylates and activates E2Fa a , Ectopic E2Fa activation of S-phase genes requires glucose-TOR signalling in leaf cells. WT or tor protoplasts expressing E2Fa-HA or S6K1-FLAG were treated without or with rapamycin (Rap) or antimycin A (AMA). QRT-PCR analyses. P-T449 indicates endogenous TOR kinase activity. Protein blot analysis (inset). b-c , TOR kinase directly phosphorylates E2Fa and 4E-BP1. Torin1 specifically inhibits TOR kinase. Staurosporine (Stau) inhibits S6K1 kinase. d , TOR directly interacts with E2Fa by immunoprecipitation (IP) and Western (W) blot analysis.

    Journal: Nature

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation

    doi: 10.1038/nature12030

    Figure Lengend Snippet: TOR kinase phosphorylates and activates E2Fa a , Ectopic E2Fa activation of S-phase genes requires glucose-TOR signalling in leaf cells. WT or tor protoplasts expressing E2Fa-HA or S6K1-FLAG were treated without or with rapamycin (Rap) or antimycin A (AMA). QRT-PCR analyses. P-T449 indicates endogenous TOR kinase activity. Protein blot analysis (inset). b-c , TOR kinase directly phosphorylates E2Fa and 4E-BP1. Torin1 specifically inhibits TOR kinase. Staurosporine (Stau) inhibits S6K1 kinase. d , TOR directly interacts with E2Fa by immunoprecipitation (IP) and Western (W) blot analysis.

    Article Snippet: All qRT-PCR analyses were performed by CFX96 real time PCR detection system with iQ SYBR green supermix (Bio-Rad).

    Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Activity Assay, Immunoprecipitation, Western Blot

    Auxin and cytokinin signalling and root stem-cell maintenance are decoupled from TOR activation a-b , Auxin signalling. c-d , Cytokinin signalling. Rapamycin (Rap), mitochondrial blocker (AMA). Primary auxin and cytokinin marker genes were activated by 1 h of indole-3-acetic acid (IAA) or trans-zeatin (tZ) treatment, and analysed by qRT-PCR. Means ± s.d., n=3. DR5::GFP or TCS::GFP was activated by 6 h of IAA or tZ treatment. Scale bar, 50 µm. e, f , Root stem-cell maintenance is TOR independent. PLT1::GFP , root stem-cell marker; WOX5::GFP : root quiescent centre marker. Scale bar, 20 µm.

    Journal: Nature

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation

    doi: 10.1038/nature12030

    Figure Lengend Snippet: Auxin and cytokinin signalling and root stem-cell maintenance are decoupled from TOR activation a-b , Auxin signalling. c-d , Cytokinin signalling. Rapamycin (Rap), mitochondrial blocker (AMA). Primary auxin and cytokinin marker genes were activated by 1 h of indole-3-acetic acid (IAA) or trans-zeatin (tZ) treatment, and analysed by qRT-PCR. Means ± s.d., n=3. DR5::GFP or TCS::GFP was activated by 6 h of IAA or tZ treatment. Scale bar, 50 µm. e, f , Root stem-cell maintenance is TOR independent. PLT1::GFP , root stem-cell marker; WOX5::GFP : root quiescent centre marker. Scale bar, 20 µm.

    Article Snippet: All qRT-PCR analyses were performed by CFX96 real time PCR detection system with iQ SYBR green supermix (Bio-Rad).

    Techniques: Activation Assay, Marker, Quantitative RT-PCR

    Glucose-TOR signalling orchestrates transcriptome reprogramming a-b , Glucose-TOR activated genes. c-d , Glucose-TOR repressed genes. 3DAG WT or tor seedlings were treated without or with glucose for 2 h. Hierarchical clustering analysis of glucose-TOR genes and five independent datasets (Glc2h, Glc4h, Sucrose, LowCO 2 -light, lowCO 2 -dark). Deep-pink/blue bar indicates novel glucose-TOR genes. The enriched functional categories highlighted in bold ( Supplementary Tables 1, 3, 4 ). e , Hierarchical clustering analysis of glucose-TOR genes (Glc) and cell cycle genes (G1, S, G2, M) 33 . f , Glucose-TOR activated genes overlap with E2Fa target genes. g , Glucose-TOR activates S-phase genes. QRT-PCR analyses. Means ± s.d., n=3. *P

    Journal: Nature

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation

    doi: 10.1038/nature12030

    Figure Lengend Snippet: Glucose-TOR signalling orchestrates transcriptome reprogramming a-b , Glucose-TOR activated genes. c-d , Glucose-TOR repressed genes. 3DAG WT or tor seedlings were treated without or with glucose for 2 h. Hierarchical clustering analysis of glucose-TOR genes and five independent datasets (Glc2h, Glc4h, Sucrose, LowCO 2 -light, lowCO 2 -dark). Deep-pink/blue bar indicates novel glucose-TOR genes. The enriched functional categories highlighted in bold ( Supplementary Tables 1, 3, 4 ). e , Hierarchical clustering analysis of glucose-TOR genes (Glc) and cell cycle genes (G1, S, G2, M) 33 . f , Glucose-TOR activated genes overlap with E2Fa target genes. g , Glucose-TOR activates S-phase genes. QRT-PCR analyses. Means ± s.d., n=3. *P

    Article Snippet: All qRT-PCR analyses were performed by CFX96 real time PCR detection system with iQ SYBR green supermix (Bio-Rad).

    Techniques: Functional Assay, Gas Chromatography, Quantitative RT-PCR

    TOR kinase controls the activity of E2Fa in transcriptional activation a , TOR kinase phosphorylates the N-terminal domain of E2Fa. b , TOR kinase phosphorylation is critical for E2Fa activation of S-phase genes. c , E2Fa-DNA binding is not affected by TOR kinase phosphorylation. ChIP-qPCR analyses with P (promoter) or G (gene body) primers. Stars, putative E2Fa-binding motifs. Error bars (n=2). d-e , Glucose responses is diminished in e2fa root meristems. Scale bar, 1 mm or 20 µm. QRT-PCR analyses. f , Model of leaf-root coordination in glucose-TOR signalling. GSH, GLUTATHIONE, RGF , ROOT GROWTH FACTOR, UPB1 , UPBEAT1. Means ± s.d., n=3. *P

    Journal: Nature

    Article Title: Glc-TOR signalling leads transcriptome reprogramming and meristem activation

    doi: 10.1038/nature12030

    Figure Lengend Snippet: TOR kinase controls the activity of E2Fa in transcriptional activation a , TOR kinase phosphorylates the N-terminal domain of E2Fa. b , TOR kinase phosphorylation is critical for E2Fa activation of S-phase genes. c , E2Fa-DNA binding is not affected by TOR kinase phosphorylation. ChIP-qPCR analyses with P (promoter) or G (gene body) primers. Stars, putative E2Fa-binding motifs. Error bars (n=2). d-e , Glucose responses is diminished in e2fa root meristems. Scale bar, 1 mm or 20 µm. QRT-PCR analyses. f , Model of leaf-root coordination in glucose-TOR signalling. GSH, GLUTATHIONE, RGF , ROOT GROWTH FACTOR, UPB1 , UPBEAT1. Means ± s.d., n=3. *P

    Article Snippet: All qRT-PCR analyses were performed by CFX96 real time PCR detection system with iQ SYBR green supermix (Bio-Rad).

    Techniques: Activity Assay, Activation Assay, Binding Assay, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    qRT-PCR analysis was performed at 1-, 3-, and 7-h post-laser treatment of the laser spot and the adjacent tissue region compared to control for the eleven up-regulated genes of interest. In addition to the genes of interest, qRT-PCR on luciferase for

    Journal: Journal of Biomedical Optics

    Article Title: Image-guided genomic analysis of tissue response to laser-induced thermal stress

    doi: 10.1117/1.3573387

    Figure Lengend Snippet: qRT-PCR analysis was performed at 1-, 3-, and 7-h post-laser treatment of the laser spot and the adjacent tissue region compared to control for the eleven up-regulated genes of interest. In addition to the genes of interest, qRT-PCR on luciferase for

    Article Snippet: Quantitative qRT-PCR analyses were performed with an iCycler iQ(Bio-Rad, Hercules, California) using Quantitect PCR Master Mix (Qiagen).

    Techniques: Quantitative RT-PCR, Luciferase

    Relative Expression Quantification by Real Time qRT-PCR

    Journal: Journal of Biomedical Optics

    Article Title: Image-guided genomic analysis of tissue response to laser-induced thermal stress

    doi: 10.1117/1.3573387

    Figure Lengend Snippet: Relative Expression Quantification by Real Time qRT-PCR

    Article Snippet: Quantitative qRT-PCR analyses were performed with an iCycler iQ(Bio-Rad, Hercules, California) using Quantitect PCR Master Mix (Qiagen).

    Techniques: Expressing, Quantitative RT-PCR

    Chloride channel-3 (CIC-3) expression in breast tumor tissues. (A) Chloride channels (ClC-1–7, CFTR) are expressed in human breast cancer tissues from clinical postoperative patients, as indicated by qRT-PCR mRNA analysis. The data are presented as relative fold changes in breast cancer tissues versus normal tissues. (B) Expression of ClC-3 was examined by western blotting, (C) ClC-3 protein is expressed in breast cancer tissues. The result shows a significant increase in ClC-3 expression in breast cancer tissues vs. normal tissues. CFTR=cystic fbrosis transmembrane conductance regulator; qRT-PCR=quantitative real - time polymerase chain reaction; mRNA=messenger RNA; GAPDH=glyceraldehyde 3-phosphate dehydrogenase. * p

    Journal: Journal of Breast Cancer

    Article Title: Knockdown of Chloride Channel-3 Inhibits Breast Cancer Growth In Vitro and In Vivo

    doi: 10.4048/jbc.2018.21.2.103

    Figure Lengend Snippet: Chloride channel-3 (CIC-3) expression in breast tumor tissues. (A) Chloride channels (ClC-1–7, CFTR) are expressed in human breast cancer tissues from clinical postoperative patients, as indicated by qRT-PCR mRNA analysis. The data are presented as relative fold changes in breast cancer tissues versus normal tissues. (B) Expression of ClC-3 was examined by western blotting, (C) ClC-3 protein is expressed in breast cancer tissues. The result shows a significant increase in ClC-3 expression in breast cancer tissues vs. normal tissues. CFTR=cystic fbrosis transmembrane conductance regulator; qRT-PCR=quantitative real - time polymerase chain reaction; mRNA=messenger RNA; GAPDH=glyceraldehyde 3-phosphate dehydrogenase. * p

    Article Snippet: Quantitative RT-PCR (qRT-PCR) analyses were carried out on a MyiQ Single Color Real-time PCR Detection System (Bio-Rad Laboratories, Hercules, USA) for 40 cycles at 95℃ for 15 seconds and 60℃ for 1 minute, after an initial incubation at 95℃ for 2 minutes.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Real-time Polymerase Chain Reaction

    Replication kinetics of influenza virus assessed by qRT-PCR. Virus replication was assessed in the absence or presence of TPCK-trypsin (1 µg/mL). Virus (1000 TCID 50 in 100 µL) was placed into a well of a 96-well plate. After incubation for 1 h at 37°C, a suspension of MDCK-London cells (30,000 in 100 µL) was added. At the indicated times, experimental samples were prepared using SPR and subjected to qRT-PCR. The RNA copy numbers were normalized to the mean value observed for A/PR/8/34 at 6 hours in the presence of TPCK-trypsin. Each point represents the mean ± SEM (n = 3).

    Journal: PLoS ONE

    Article Title: Development of a Neutralization Assay for Influenza Virus Using an Endpoint Assessment Based on Quantitative Reverse-Transcription PCR

    doi: 10.1371/journal.pone.0056023

    Figure Lengend Snippet: Replication kinetics of influenza virus assessed by qRT-PCR. Virus replication was assessed in the absence or presence of TPCK-trypsin (1 µg/mL). Virus (1000 TCID 50 in 100 µL) was placed into a well of a 96-well plate. After incubation for 1 h at 37°C, a suspension of MDCK-London cells (30,000 in 100 µL) was added. At the indicated times, experimental samples were prepared using SPR and subjected to qRT-PCR. The RNA copy numbers were normalized to the mean value observed for A/PR/8/34 at 6 hours in the presence of TPCK-trypsin. Each point represents the mean ± SEM (n = 3).

    Article Snippet: Performance Analysis of SYBR-Green qRT-PCR We have made use of a commercially available reagent, the Bio-Rad iScript Sample Preparation Reagent (referred to hereafter as SPR), to prepare cell lysates amenable for direct assessment by downstream quantitative reverse transcription PCR (qRT-PCR) with minimal attendant processing steps.

    Techniques: Quantitative RT-PCR, Incubation, SPR Assay

    Discrimination of 2-fold variations in virus input. Virus replication was assessed in the presence of TPCK-trypsin (1 µg/mL). A 2-fold dilution series of the virus was prepared (32,000 to 250 TCID 50 per 100 µL per well). After incubation for 1 h at 37°C, a suspension of MDCK-London cells (30,000 in 100 µL) was added. At 6 h post-infection, experimental samples were prepared using SPR and subjected to qRT-PCR. For each virus strain, the RNA copy numbers were normalized to the mean value observed from cells infected with 250 TCID 50 . Each point represents the mean ± SEM (n = 3).

    Journal: PLoS ONE

    Article Title: Development of a Neutralization Assay for Influenza Virus Using an Endpoint Assessment Based on Quantitative Reverse-Transcription PCR

    doi: 10.1371/journal.pone.0056023

    Figure Lengend Snippet: Discrimination of 2-fold variations in virus input. Virus replication was assessed in the presence of TPCK-trypsin (1 µg/mL). A 2-fold dilution series of the virus was prepared (32,000 to 250 TCID 50 per 100 µL per well). After incubation for 1 h at 37°C, a suspension of MDCK-London cells (30,000 in 100 µL) was added. At 6 h post-infection, experimental samples were prepared using SPR and subjected to qRT-PCR. For each virus strain, the RNA copy numbers were normalized to the mean value observed from cells infected with 250 TCID 50 . Each point represents the mean ± SEM (n = 3).

    Article Snippet: Performance Analysis of SYBR-Green qRT-PCR We have made use of a commercially available reagent, the Bio-Rad iScript Sample Preparation Reagent (referred to hereafter as SPR), to prepare cell lysates amenable for direct assessment by downstream quantitative reverse transcription PCR (qRT-PCR) with minimal attendant processing steps.

    Techniques: Incubation, Infection, SPR Assay, Quantitative RT-PCR

    Influenza virus microneutralization assessed by qRT-PCR (qPCR-MN). ( A ) An inoculum containing 1000 TCID 50 of virus (Bris/07) was mixed with a dilution from a 2-fold dilution series of ferret antiserum in a well of a 96-well plate. After allowing the neutralization reaction to proceed for 1 hour at 37°C, trypsinized MDCK-London cells (30,000 per well) were added. TPCK-trypsin was present at 1 µg/mL. After 6 hours, experimental samples were prepared using SPR and subjected to qRT-PCR. The RNA copy numbers were normalized to the mean value obtained from infected wells in the absence of neutralizing serum (virus control wells). Each point represents the mean ± SEM (n = 3). The neutralization titer was defined as the reciprocal of the highest dilution factor of serum necessary to inhibit the PCR signal by 90%. ( B ) Same data as in (A); however, each experimental replicate was assessed independently. The mean of these curves would result in the curve depicted in (A).

    Journal: PLoS ONE

    Article Title: Development of a Neutralization Assay for Influenza Virus Using an Endpoint Assessment Based on Quantitative Reverse-Transcription PCR

    doi: 10.1371/journal.pone.0056023

    Figure Lengend Snippet: Influenza virus microneutralization assessed by qRT-PCR (qPCR-MN). ( A ) An inoculum containing 1000 TCID 50 of virus (Bris/07) was mixed with a dilution from a 2-fold dilution series of ferret antiserum in a well of a 96-well plate. After allowing the neutralization reaction to proceed for 1 hour at 37°C, trypsinized MDCK-London cells (30,000 per well) were added. TPCK-trypsin was present at 1 µg/mL. After 6 hours, experimental samples were prepared using SPR and subjected to qRT-PCR. The RNA copy numbers were normalized to the mean value obtained from infected wells in the absence of neutralizing serum (virus control wells). Each point represents the mean ± SEM (n = 3). The neutralization titer was defined as the reciprocal of the highest dilution factor of serum necessary to inhibit the PCR signal by 90%. ( B ) Same data as in (A); however, each experimental replicate was assessed independently. The mean of these curves would result in the curve depicted in (A).

    Article Snippet: Performance Analysis of SYBR-Green qRT-PCR We have made use of a commercially available reagent, the Bio-Rad iScript Sample Preparation Reagent (referred to hereafter as SPR), to prepare cell lysates amenable for direct assessment by downstream quantitative reverse transcription PCR (qRT-PCR) with minimal attendant processing steps.

    Techniques: Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Neutralization, SPR Assay, Infection, Polymerase Chain Reaction

    Threshold cycle (C t ) vs . log 10 (dilution factor) from SYBR Green qRT-PCR (targeting the M1 gene of influenza A viruses) applied to a serial dilution of total RNA purified from MDCK-London cells infected with A/PR/8/34 (n = 3 for each dilution), which was used as an RNA quantification standard for our experiments. Lysate from uninfected MDCK-London cells prepared with the Bio-Rad iScript Sample Preparation Reagent (SPR) was used as the diluent to prepare the RNA serial dilution in order to achieve comparability with experimental samples. The initial dilution contained 10 ng of standard RNA per µL.

    Journal: PLoS ONE

    Article Title: Development of a Neutralization Assay for Influenza Virus Using an Endpoint Assessment Based on Quantitative Reverse-Transcription PCR

    doi: 10.1371/journal.pone.0056023

    Figure Lengend Snippet: Threshold cycle (C t ) vs . log 10 (dilution factor) from SYBR Green qRT-PCR (targeting the M1 gene of influenza A viruses) applied to a serial dilution of total RNA purified from MDCK-London cells infected with A/PR/8/34 (n = 3 for each dilution), which was used as an RNA quantification standard for our experiments. Lysate from uninfected MDCK-London cells prepared with the Bio-Rad iScript Sample Preparation Reagent (SPR) was used as the diluent to prepare the RNA serial dilution in order to achieve comparability with experimental samples. The initial dilution contained 10 ng of standard RNA per µL.

    Article Snippet: Performance Analysis of SYBR-Green qRT-PCR We have made use of a commercially available reagent, the Bio-Rad iScript Sample Preparation Reagent (referred to hereafter as SPR), to prepare cell lysates amenable for direct assessment by downstream quantitative reverse transcription PCR (qRT-PCR) with minimal attendant processing steps.

    Techniques: SYBR Green Assay, Quantitative RT-PCR, Serial Dilution, Purification, Infection, Sample Prep, SPR Assay

    Relative expression of strawberry genes ( FaPE1 , FaPLA , FaPG1 , FaPG2 , FaOMT , FaQR , and FaGalUR ), as determined by qRT–PCR, in the achenes and receptacles of ripe strawberry fruits. Asterisks indicate significant differences between the transgenic lines and the control for each sample using ANOVA and the Tukey HSD test adjusted to a 95% significance level. Different letters indicate significant differences within the control lines (achene and receptacle) for each gene using ANOVA and the Tukey HSD test adjusted to a 95% significance level.

    Journal: Journal of Experimental Botany

    Article Title: Ethylene is involved in strawberry fruit ripening in an organ-specific manner

    doi: 10.1093/jxb/ert257

    Figure Lengend Snippet: Relative expression of strawberry genes ( FaPE1 , FaPLA , FaPG1 , FaPG2 , FaOMT , FaQR , and FaGalUR ), as determined by qRT–PCR, in the achenes and receptacles of ripe strawberry fruits. Asterisks indicate significant differences between the transgenic lines and the control for each sample using ANOVA and the Tukey HSD test adjusted to a 95% significance level. Different letters indicate significant differences within the control lines (achene and receptacle) for each gene using ANOVA and the Tukey HSD test adjusted to a 95% significance level.

    Article Snippet: The expression of the genes encoding various ethylene receptors (etr1-1 , FaETR1 , FaETR2 , and FaERS1 ), phenylalanine ammonia lyase (FaPAL ), chalcone synthase (FaCHS ), 1-aminocyclopropane-1-carboxylate oxidase 1 (FaACO1 ), 1-aminocyclopropane-1-carboxylate oxidase 2 (FaACO2 ), 1-aminocyclopropane-1-carboxylate oxidase 3(FaACO3 ), 1-aminocyclopropane-1-carboxylate synthase 1 (FaACS1 ), 1-aminocyclopropane-1-carboxylate synthase 2 (FaACS2 ), 1-aminocyclopropane-1-carboxylate synthase 3 (FaACS3 ), 1-aminocyclopropane-1-carboxylate synthase 4 (FaACS4 ), pectin methyl esterase (FaPE1 ), pectate lyase A (FaPLA ), polygalacturonase 1 and 2 (FaPG1 and FaPG2 ), O -methyltransferase (FaOMT ), quinone reductase (FaQR ), MYB transcription factors (FaMYB1 and FaMYB10 ), and d -galacturnonate reductase (FaGalUR ), was analysed by real-time qRT–PCR using the fluorescent intercalating dye EvaGreen in an iCycler detection system (Bio-Rad).

    Techniques: Expressing, Quantitative RT-PCR, Transgenic Assay

    Relative expression of strawberry genes ( FaPAL , FaCHS , FaMYB1 , and FaMYB1 ), as determined by qRT–PCR, in the achenes and receptacles of ripe strawberry fruits. Asterisks indicate significant differences between the transgenic lines and the control for each sample using ANOVA and the Tukey HSD test adjusted to a 95% significance level. Different letters indicate significant differences within the control lines (achene and receptacle) for each gene using ANOVA and the Tukey HSD test adjusted to a 95% significance level.

    Journal: Journal of Experimental Botany

    Article Title: Ethylene is involved in strawberry fruit ripening in an organ-specific manner

    doi: 10.1093/jxb/ert257

    Figure Lengend Snippet: Relative expression of strawberry genes ( FaPAL , FaCHS , FaMYB1 , and FaMYB1 ), as determined by qRT–PCR, in the achenes and receptacles of ripe strawberry fruits. Asterisks indicate significant differences between the transgenic lines and the control for each sample using ANOVA and the Tukey HSD test adjusted to a 95% significance level. Different letters indicate significant differences within the control lines (achene and receptacle) for each gene using ANOVA and the Tukey HSD test adjusted to a 95% significance level.

    Article Snippet: The expression of the genes encoding various ethylene receptors (etr1-1 , FaETR1 , FaETR2 , and FaERS1 ), phenylalanine ammonia lyase (FaPAL ), chalcone synthase (FaCHS ), 1-aminocyclopropane-1-carboxylate oxidase 1 (FaACO1 ), 1-aminocyclopropane-1-carboxylate oxidase 2 (FaACO2 ), 1-aminocyclopropane-1-carboxylate oxidase 3(FaACO3 ), 1-aminocyclopropane-1-carboxylate synthase 1 (FaACS1 ), 1-aminocyclopropane-1-carboxylate synthase 2 (FaACS2 ), 1-aminocyclopropane-1-carboxylate synthase 3 (FaACS3 ), 1-aminocyclopropane-1-carboxylate synthase 4 (FaACS4 ), pectin methyl esterase (FaPE1 ), pectate lyase A (FaPLA ), polygalacturonase 1 and 2 (FaPG1 and FaPG2 ), O -methyltransferase (FaOMT ), quinone reductase (FaQR ), MYB transcription factors (FaMYB1 and FaMYB10 ), and d -galacturnonate reductase (FaGalUR ), was analysed by real-time qRT–PCR using the fluorescent intercalating dye EvaGreen in an iCycler detection system (Bio-Rad).

    Techniques: Expressing, Quantitative RT-PCR, Transgenic Assay

    Expression of the Arabidopsis etr1-1 gene in the fruits of the transgenic lines and analysis of the lower sensitivity of these lines to ethylene. (A) Relative expression of etr1-1 , as determined by qRT–PCR, in the achenes and receptacles of the green and ripe stages of the transgenic lines (L10, L12, and L15) and control (C). (B) Photographs of control and etr1-1 plantlets obtained after germination of achenes in normal medium (MS) and medium supplemented with 100 μM ACC. (C) Photographs of 4-week-old in vitro control and L12 plants grown in standard (N30K) medium and medium supplemented with 10 μM ACC.

    Journal: Journal of Experimental Botany

    Article Title: Ethylene is involved in strawberry fruit ripening in an organ-specific manner

    doi: 10.1093/jxb/ert257

    Figure Lengend Snippet: Expression of the Arabidopsis etr1-1 gene in the fruits of the transgenic lines and analysis of the lower sensitivity of these lines to ethylene. (A) Relative expression of etr1-1 , as determined by qRT–PCR, in the achenes and receptacles of the green and ripe stages of the transgenic lines (L10, L12, and L15) and control (C). (B) Photographs of control and etr1-1 plantlets obtained after germination of achenes in normal medium (MS) and medium supplemented with 100 μM ACC. (C) Photographs of 4-week-old in vitro control and L12 plants grown in standard (N30K) medium and medium supplemented with 10 μM ACC.

    Article Snippet: The expression of the genes encoding various ethylene receptors (etr1-1 , FaETR1 , FaETR2 , and FaERS1 ), phenylalanine ammonia lyase (FaPAL ), chalcone synthase (FaCHS ), 1-aminocyclopropane-1-carboxylate oxidase 1 (FaACO1 ), 1-aminocyclopropane-1-carboxylate oxidase 2 (FaACO2 ), 1-aminocyclopropane-1-carboxylate oxidase 3(FaACO3 ), 1-aminocyclopropane-1-carboxylate synthase 1 (FaACS1 ), 1-aminocyclopropane-1-carboxylate synthase 2 (FaACS2 ), 1-aminocyclopropane-1-carboxylate synthase 3 (FaACS3 ), 1-aminocyclopropane-1-carboxylate synthase 4 (FaACS4 ), pectin methyl esterase (FaPE1 ), pectate lyase A (FaPLA ), polygalacturonase 1 and 2 (FaPG1 and FaPG2 ), O -methyltransferase (FaOMT ), quinone reductase (FaQR ), MYB transcription factors (FaMYB1 and FaMYB10 ), and d -galacturnonate reductase (FaGalUR ), was analysed by real-time qRT–PCR using the fluorescent intercalating dye EvaGreen in an iCycler detection system (Bio-Rad).

    Techniques: Expressing, Transgenic Assay, Quantitative RT-PCR, Mass Spectrometry, In Vitro

    Analysis of expression of the genes encoding ACC synthase ( ACS ) and ACC oxidase ( ACO ) in the strawberry Fragaria×ananassa . (A and C) Expression of ACS and ACO , as determined by qRT–PCR, in the achenes and receptacles of strawberry fruits at three developmental stages (G, green; W, white; R, red). (B and D) Unrooted phylogenetic tree of the ACS and ACO proteins of F.×ananassa (Fa), F. vesca (Fv), and Solanum lycopersicum (Sl). Different letters indicate significant differences, in achenes and receptacles, for each gene using ANOVA and the Tukey HSD test adjusted to a 95% significance level.

    Journal: Journal of Experimental Botany

    Article Title: Ethylene is involved in strawberry fruit ripening in an organ-specific manner

    doi: 10.1093/jxb/ert257

    Figure Lengend Snippet: Analysis of expression of the genes encoding ACC synthase ( ACS ) and ACC oxidase ( ACO ) in the strawberry Fragaria×ananassa . (A and C) Expression of ACS and ACO , as determined by qRT–PCR, in the achenes and receptacles of strawberry fruits at three developmental stages (G, green; W, white; R, red). (B and D) Unrooted phylogenetic tree of the ACS and ACO proteins of F.×ananassa (Fa), F. vesca (Fv), and Solanum lycopersicum (Sl). Different letters indicate significant differences, in achenes and receptacles, for each gene using ANOVA and the Tukey HSD test adjusted to a 95% significance level.

    Article Snippet: The expression of the genes encoding various ethylene receptors (etr1-1 , FaETR1 , FaETR2 , and FaERS1 ), phenylalanine ammonia lyase (FaPAL ), chalcone synthase (FaCHS ), 1-aminocyclopropane-1-carboxylate oxidase 1 (FaACO1 ), 1-aminocyclopropane-1-carboxylate oxidase 2 (FaACO2 ), 1-aminocyclopropane-1-carboxylate oxidase 3(FaACO3 ), 1-aminocyclopropane-1-carboxylate synthase 1 (FaACS1 ), 1-aminocyclopropane-1-carboxylate synthase 2 (FaACS2 ), 1-aminocyclopropane-1-carboxylate synthase 3 (FaACS3 ), 1-aminocyclopropane-1-carboxylate synthase 4 (FaACS4 ), pectin methyl esterase (FaPE1 ), pectate lyase A (FaPLA ), polygalacturonase 1 and 2 (FaPG1 and FaPG2 ), O -methyltransferase (FaOMT ), quinone reductase (FaQR ), MYB transcription factors (FaMYB1 and FaMYB10 ), and d -galacturnonate reductase (FaGalUR ), was analysed by real-time qRT–PCR using the fluorescent intercalating dye EvaGreen in an iCycler detection system (Bio-Rad).

    Techniques: Expressing, Quantitative RT-PCR

    Relative expression of ethylene receptor genes ( FaETR1 , FaETR2 , and FaERS1 ), as determined by qRT–PCR, in the achenes and receptacles of ripe strawberry fruits. Asterisks indicate significant differences between the transgenic lines and the control for each sample using ANOVA and the Tukey HSD test adjusted to a 95% significance level. Different letters indicate significant differences within the control lines (achene and receptacle) for each gene using ANOVA and the Tukey HSD test adjusted to a 95% significance level.

    Journal: Journal of Experimental Botany

    Article Title: Ethylene is involved in strawberry fruit ripening in an organ-specific manner

    doi: 10.1093/jxb/ert257

    Figure Lengend Snippet: Relative expression of ethylene receptor genes ( FaETR1 , FaETR2 , and FaERS1 ), as determined by qRT–PCR, in the achenes and receptacles of ripe strawberry fruits. Asterisks indicate significant differences between the transgenic lines and the control for each sample using ANOVA and the Tukey HSD test adjusted to a 95% significance level. Different letters indicate significant differences within the control lines (achene and receptacle) for each gene using ANOVA and the Tukey HSD test adjusted to a 95% significance level.

    Article Snippet: The expression of the genes encoding various ethylene receptors (etr1-1 , FaETR1 , FaETR2 , and FaERS1 ), phenylalanine ammonia lyase (FaPAL ), chalcone synthase (FaCHS ), 1-aminocyclopropane-1-carboxylate oxidase 1 (FaACO1 ), 1-aminocyclopropane-1-carboxylate oxidase 2 (FaACO2 ), 1-aminocyclopropane-1-carboxylate oxidase 3(FaACO3 ), 1-aminocyclopropane-1-carboxylate synthase 1 (FaACS1 ), 1-aminocyclopropane-1-carboxylate synthase 2 (FaACS2 ), 1-aminocyclopropane-1-carboxylate synthase 3 (FaACS3 ), 1-aminocyclopropane-1-carboxylate synthase 4 (FaACS4 ), pectin methyl esterase (FaPE1 ), pectate lyase A (FaPLA ), polygalacturonase 1 and 2 (FaPG1 and FaPG2 ), O -methyltransferase (FaOMT ), quinone reductase (FaQR ), MYB transcription factors (FaMYB1 and FaMYB10 ), and d -galacturnonate reductase (FaGalUR ), was analysed by real-time qRT–PCR using the fluorescent intercalating dye EvaGreen in an iCycler detection system (Bio-Rad).

    Techniques: Expressing, Quantitative RT-PCR, Transgenic Assay

    Notch target gene expression is deregulated in primary Ikaros null thymocytes in the absence of deregulated ICN production. A , qRT-PCR analysis was performed using cDNA prepared from sorted thymocyte subsets (double negative, DN; double positive, DP)

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Ikaros Regulates Notch Target Gene Expression in Developing Thymocytes 1

    doi:

    Figure Lengend Snippet: Notch target gene expression is deregulated in primary Ikaros null thymocytes in the absence of deregulated ICN production. A , qRT-PCR analysis was performed using cDNA prepared from sorted thymocyte subsets (double negative, DN; double positive, DP)

    Article Snippet: RNA was prepared from sorted primary thymocytes or from retrovirally transduced JE131, DO11, and Tu5 cells (sorted for H-2Kk expression) at 24 h postinfection using the SV Total RNA Isolation System (Promega). cDNA was generated with a Superscript III kit (Invitrogen), and used for quantitative real-time RT-PCR (qRT-PCR) analyses (Bio Rad iQ5 Real Time PCR machine).

    Techniques: Expressing, Quantitative RT-PCR

    Deregulation of Notch target genes is observed in Ikaros null single positive thymocytes. qRT-PCR analysis was performed using cDNA prepared from sorted CD4 + and CD8 + ) thymocyte subsets from Ikaros null (Ik − / − ) and

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Ikaros Regulates Notch Target Gene Expression in Developing Thymocytes 1

    doi:

    Figure Lengend Snippet: Deregulation of Notch target genes is observed in Ikaros null single positive thymocytes. qRT-PCR analysis was performed using cDNA prepared from sorted CD4 + and CD8 + ) thymocyte subsets from Ikaros null (Ik − / − ) and

    Article Snippet: RNA was prepared from sorted primary thymocytes or from retrovirally transduced JE131, DO11, and Tu5 cells (sorted for H-2Kk expression) at 24 h postinfection using the SV Total RNA Isolation System (Promega). cDNA was generated with a Superscript III kit (Invitrogen), and used for quantitative real-time RT-PCR (qRT-PCR) analyses (Bio Rad iQ5 Real Time PCR machine).

    Techniques: Quantitative RT-PCR

    Ikaros null bone marrow progenitors do not display derepressed Notch target gene expression. A , qRT-PCR was performed using cDNA prepared from lineage depleted bone marrow from Ikaros null (Ik −/− ) and wild-type (Ik +/+ ) mice. Expression

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Ikaros Regulates Notch Target Gene Expression in Developing Thymocytes 1

    doi:

    Figure Lengend Snippet: Ikaros null bone marrow progenitors do not display derepressed Notch target gene expression. A , qRT-PCR was performed using cDNA prepared from lineage depleted bone marrow from Ikaros null (Ik −/− ) and wild-type (Ik +/+ ) mice. Expression

    Article Snippet: RNA was prepared from sorted primary thymocytes or from retrovirally transduced JE131, DO11, and Tu5 cells (sorted for H-2Kk expression) at 24 h postinfection using the SV Total RNA Isolation System (Promega). cDNA was generated with a Superscript III kit (Invitrogen), and used for quantitative real-time RT-PCR (qRT-PCR) analyses (Bio Rad iQ5 Real Time PCR machine).

    Techniques: Expressing, Quantitative RT-PCR, Mouse Assay

    Relative expression of P. eryngii KNR2312 receptors (a) and pheromones (b) in the monokaryon (P6) and dikaryon (KNR2312), as determined by real-time qRT-PCR. Gene expression was normalized to 18S rRNA expression and calibrated to the value for the monokaryon (P6), which was assigned a value of 1, by the standard curve method (Bio-Rad). All assays were performed in triplicate. The error bars show the standard deviations for triplicate samples.

    Journal: PLoS ONE

    Article Title: Identification and Functional Analysis of Pheromone and Receptor Genes in the B3 Mating Locus of Pleurotus eryngii

    doi: 10.1371/journal.pone.0104693

    Figure Lengend Snippet: Relative expression of P. eryngii KNR2312 receptors (a) and pheromones (b) in the monokaryon (P6) and dikaryon (KNR2312), as determined by real-time qRT-PCR. Gene expression was normalized to 18S rRNA expression and calibrated to the value for the monokaryon (P6), which was assigned a value of 1, by the standard curve method (Bio-Rad). All assays were performed in triplicate. The error bars show the standard deviations for triplicate samples.

    Article Snippet: Analysis of gene expression by real-time qRT-PCR Real-time qRT-PCR (CFX96, Bio-Rad) was performed using KNR2312 (dikaryon) and P6 (monokaryon) to quantify the expression of the 4 receptor genes and the 4 pheromone genes.

    Techniques: Expressing, Quantitative RT-PCR

    Mediator-independent and TAF6 and AF9-dependent interaction of TFIID and SEC in mammalian cells. A. Stable knockdown of TAF6 in 293T cells using TAF6-specific shRNA. Knockdown efficiency was tested by western blotting (upper panel) as well as RNA analysis by qRT-PCR. B. Stable knockdown of AF9 in 293T cells using AF9-specific shRNA. Knockdown efficiency was tested by western blotting (upper panel) as well as RNA analysis by qRT-PCR. C. Schematics of experimental strategy that has been employed for experiments mentioned in the panel D and E. D. Immunoblot analysis showing the effect of TAF6 knockdown on association of other TAF and SEC subunits with ectopically expressed FLAG-TBP. TBP-associated proteins were pulled-down using anti-FLAG M2 beads and proteins were identified by immunoblotting using specific antibodies. E. Immunoblot analysis showing the effect of AF9 knockdown on association of other TAF and SEC subunits with ectopically expressed TBP. TBP-associated proteins were pulled-down using anti-FLAG M2 beads and proteins were identified by immunoblotting using specific antibodies. F. Stable knockdown of Med26 in 293T cells using Med26-specific shRNA. Knockdown efficiency was tested by western blotting. G. Immunoblot analysis showing the effect of Med26 knockdown on association of other TAF and SEC subunits with ectopically expressed FLAG-TBP. TBP-associated proteins were pulled-down using anti-FLAG M2 beads and proteins were identified by immunoblotting using specific antibodies. The left panel shows experimental strategy that was used for this assay. H. Immunoblot analysis showing the effect of Med26 knockdown on association of other TAF and SEC subunits with ectopically expressed EAF1. EAF1-associated proteins were pulled-down using anti-FLAG M2 beads and proteins were identified by immunoblotting using specific antibodies. The left panel shows experimental strategy that was used for this assay.

    Journal: Cell reports

    Article Title: Multivalent role of human TFIID in recruiting elongation components at the promoter proximal region for transcriptional control

    doi: 10.1016/j.celrep.2019.01.012

    Figure Lengend Snippet: Mediator-independent and TAF6 and AF9-dependent interaction of TFIID and SEC in mammalian cells. A. Stable knockdown of TAF6 in 293T cells using TAF6-specific shRNA. Knockdown efficiency was tested by western blotting (upper panel) as well as RNA analysis by qRT-PCR. B. Stable knockdown of AF9 in 293T cells using AF9-specific shRNA. Knockdown efficiency was tested by western blotting (upper panel) as well as RNA analysis by qRT-PCR. C. Schematics of experimental strategy that has been employed for experiments mentioned in the panel D and E. D. Immunoblot analysis showing the effect of TAF6 knockdown on association of other TAF and SEC subunits with ectopically expressed FLAG-TBP. TBP-associated proteins were pulled-down using anti-FLAG M2 beads and proteins were identified by immunoblotting using specific antibodies. E. Immunoblot analysis showing the effect of AF9 knockdown on association of other TAF and SEC subunits with ectopically expressed TBP. TBP-associated proteins were pulled-down using anti-FLAG M2 beads and proteins were identified by immunoblotting using specific antibodies. F. Stable knockdown of Med26 in 293T cells using Med26-specific shRNA. Knockdown efficiency was tested by western blotting. G. Immunoblot analysis showing the effect of Med26 knockdown on association of other TAF and SEC subunits with ectopically expressed FLAG-TBP. TBP-associated proteins were pulled-down using anti-FLAG M2 beads and proteins were identified by immunoblotting using specific antibodies. The left panel shows experimental strategy that was used for this assay. H. Immunoblot analysis showing the effect of Med26 knockdown on association of other TAF and SEC subunits with ectopically expressed EAF1. EAF1-associated proteins were pulled-down using anti-FLAG M2 beads and proteins were identified by immunoblotting using specific antibodies. The left panel shows experimental strategy that was used for this assay.

    Article Snippet: 1ug of RNA was reverse transcribed using verso cDNA synthesis kit (Thermo Scientific) using manufacturer's protocol. cDNA was diluted 25X for quantitative real-time PCR (qRT-PCR) analyses. qRT-PCR was performed using Universal SYBR green supermix (BIORAD).

    Techniques: Size-exclusion Chromatography, shRNA, Western Blot, Quantitative RT-PCR

    TFIID-mediated recruitment of SEC at promoter proximal region is required for basal level as well as inducible expression of target genes. A. qRT-PCR analysis showing effect of TAF6 and AF9 knockdown on basal level expression of target genes when compared to control scramble knockdown cells. Statistical significance for RNA analysis in TAF6 and AF9 knockdown samples are shown over control (Scramble) sample. B-D: ChIP analysis showing recruitment of several factors at promoter proximal region of indicated target genes upon knockdown of TAF6 and AF9. Statistical significance for ChIP analysis in TAF6 and AF9 knockdown samples are shown over control (Scramble) sample. E. qRT-PCR analysis showing effect of AF9 knockdown on induced expression of target p21 gene at different time points after doxorubicin treatment. F. ChIP analysis showing effect of AF9 knockdown on recruitment of target factors at promoter proximal and ~4kb downstream (coding) region of p21 gene after 16 hrs of doxorubicin treatment. Statistical significance for ChIP analysis in AF9 knockdown sample are shown over control (Scramble) sample. PP denotes promoter proximal region. All of our RNA expression and ChIP data represents mean ± SEM., a minimum of two biological and three PCR replicates. Statistical analysis was performed using one-tailed Student's t test wherein * denotes p ≤0.05, ** denotes p ≤0.01, *** denotes p ≤0.001, and ns denotes not significant.

    Journal: Cell reports

    Article Title: Multivalent role of human TFIID in recruiting elongation components at the promoter proximal region for transcriptional control

    doi: 10.1016/j.celrep.2019.01.012

    Figure Lengend Snippet: TFIID-mediated recruitment of SEC at promoter proximal region is required for basal level as well as inducible expression of target genes. A. qRT-PCR analysis showing effect of TAF6 and AF9 knockdown on basal level expression of target genes when compared to control scramble knockdown cells. Statistical significance for RNA analysis in TAF6 and AF9 knockdown samples are shown over control (Scramble) sample. B-D: ChIP analysis showing recruitment of several factors at promoter proximal region of indicated target genes upon knockdown of TAF6 and AF9. Statistical significance for ChIP analysis in TAF6 and AF9 knockdown samples are shown over control (Scramble) sample. E. qRT-PCR analysis showing effect of AF9 knockdown on induced expression of target p21 gene at different time points after doxorubicin treatment. F. ChIP analysis showing effect of AF9 knockdown on recruitment of target factors at promoter proximal and ~4kb downstream (coding) region of p21 gene after 16 hrs of doxorubicin treatment. Statistical significance for ChIP analysis in AF9 knockdown sample are shown over control (Scramble) sample. PP denotes promoter proximal region. All of our RNA expression and ChIP data represents mean ± SEM., a minimum of two biological and three PCR replicates. Statistical analysis was performed using one-tailed Student's t test wherein * denotes p ≤0.05, ** denotes p ≤0.01, *** denotes p ≤0.001, and ns denotes not significant.

    Article Snippet: 1ug of RNA was reverse transcribed using verso cDNA synthesis kit (Thermo Scientific) using manufacturer's protocol. cDNA was diluted 25X for quantitative real-time PCR (qRT-PCR) analyses. qRT-PCR was performed using Universal SYBR green supermix (BIORAD).

    Techniques: Size-exclusion Chromatography, Expressing, Quantitative RT-PCR, Chromatin Immunoprecipitation, RNA Expression, Polymerase Chain Reaction, One-tailed Test

    PRMT5 knockdown represses FGFR3 and eIF4E expression and decreases H3R8 and H4R3 methylation on their promoters A. HCT116, SW620, and SW480 cells were transfected with si-NC or si-PRMT5. FGFR3 and eIF4E protein were analyzed by immunoblot 72 h later. B. FGFR3 and eIF4E mRNA expression in CRC tissues was analyzed by qRT-PCR. C. ChIP assays were performed in SW480 cells with antibodies (PRMT5, H3R8, H4R3 and normal IgG), and qRT-PCR with primers targeting control region or part of FGFR3 or eIF4E gene promoter. D. SW480 cell were transfected with si-NC or si-PRMT5 and protein expression of H4R3me2s and H3R8me2s was analyzed by Western blot 72 h later. E. SW480 cell were transfected with si-NC or si-PRMT5 and the expression of indicated proteins was analyzed by Western blot 72 h later. F. Proposed molecular mechanisms by which PRMT5 promotes CRC. PRMT5 overexpression promotes the activation of FGFR3, AKT, mTOR, ERK and eIF4E, leading to CRC cell growth, survival and migration. PRMT5 knockdown or inhibition by AMI-1 results in the downregulation of FGFR3, AKT, mTOR, ERK and eIF4E, leading to the inhibition of CRC cell growth, survival and migration.

    Journal: Oncotarget

    Article Title: Targeting protein arginine methyltransferase 5 inhibits colorectal cancer growth by decreasing arginine methylation of eIF4E and FGFR3

    doi:

    Figure Lengend Snippet: PRMT5 knockdown represses FGFR3 and eIF4E expression and decreases H3R8 and H4R3 methylation on their promoters A. HCT116, SW620, and SW480 cells were transfected with si-NC or si-PRMT5. FGFR3 and eIF4E protein were analyzed by immunoblot 72 h later. B. FGFR3 and eIF4E mRNA expression in CRC tissues was analyzed by qRT-PCR. C. ChIP assays were performed in SW480 cells with antibodies (PRMT5, H3R8, H4R3 and normal IgG), and qRT-PCR with primers targeting control region or part of FGFR3 or eIF4E gene promoter. D. SW480 cell were transfected with si-NC or si-PRMT5 and protein expression of H4R3me2s and H3R8me2s was analyzed by Western blot 72 h later. E. SW480 cell were transfected with si-NC or si-PRMT5 and the expression of indicated proteins was analyzed by Western blot 72 h later. F. Proposed molecular mechanisms by which PRMT5 promotes CRC. PRMT5 overexpression promotes the activation of FGFR3, AKT, mTOR, ERK and eIF4E, leading to CRC cell growth, survival and migration. PRMT5 knockdown or inhibition by AMI-1 results in the downregulation of FGFR3, AKT, mTOR, ERK and eIF4E, leading to the inhibition of CRC cell growth, survival and migration.

    Article Snippet: Quantitative real-time PCR (qRT-PCR) analyses were carried out to detect mRNA expression using iQ™ SYBR® Green Supermix (Bio-Rad), and β-actin gene was used as an internal control.

    Techniques: Expressing, Methylation, Transfection, Quantitative RT-PCR, Chromatin Immunoprecipitation, Western Blot, Over Expression, Activation Assay, Migration, Inhibition

    Expression analysis of five genes in cotton anthers by qRT-PCR. The housekeeping gene 18S was used as an internal control; H276A, CMS line; H276B, maintainer line; and H276A/H268, F1 plants from the descendant of restorer H268 hybridized with H276A. Error bars represent standard deviation (n = 3). Double asterisk represents significance at 1% probability

    Journal: Biological Research

    Article Title: RNA editing analysis of ATP synthase genes in the cotton cytoplasmic male sterile line H276A

    doi: 10.1186/s40659-019-0212-0

    Figure Lengend Snippet: Expression analysis of five genes in cotton anthers by qRT-PCR. The housekeeping gene 18S was used as an internal control; H276A, CMS line; H276B, maintainer line; and H276A/H268, F1 plants from the descendant of restorer H268 hybridized with H276A. Error bars represent standard deviation (n = 3). Double asterisk represents significance at 1% probability

    Article Snippet: qRT-PCR analysis of ATP synthase genes Relative quantification of five genes in the three cotton lines were conducted by real-time qRT-PCR analysis with a C1000 TouchTM Thermal Cycler (Bio-Rad, USA) with TransStartR Tip Green qPCR SuperMix (Trans, China).

    Techniques: Expressing, Quantitative RT-PCR, Standard Deviation

    Generation and analysis of mice expressing A-USF. (A) DNA construct pITRp543f2A-USF4 used to generate transgenic mice expressing dominant-negative USF (A-USF). The A-USF coding region is under the control of the human β-globin gene promoter (β-P) and 3′ enhancer (3′E) as well as human LCR elements HS2 and HS3 plus flanking DNA. The DNA construct is flanked on either site by insulator elements derived from the chicken β-globin gene locus (cHS4). (B) SYBR green stain of the RT-PCR analysis of A-USF expression in transgenic (founders I and III and line II F 1 littermates 1 to 3 [II/1 to II/3]) and wild-type (WT) mice. RNA was isolated from the spleens of phenylhydrazine-treated mice, reverse transcribed, subjected to PCR analysis with primers specific to the A-USF coding region, and electrophoresed in 5% Tris-borate-EDTA polyacrylamide gels. (C) Western blot analysis of A-USF expression in transgenic or wild-type mice. Protein was isolated from the spleen or liver of phenylhydrazine-treated mice and subjected to Western blot analysis using an antibody against USF1, which also detects A-USF. (D) qRT-PCR analysis of β maj -globin gene expression in spleens of A-USF transgenic line II F 1 littermates, A-USF founder mouse I, and wild-type mice. Data from the three line II F 1 littermates were combined and are designated II/1/2/3. GAPDH was used as a loading control, and results from samples were normalized to those of the wild type. (E) ChIP analysis of RNA Pol II and USF2 interactions with the β maj -globin gene promoter control mice (WT) and transgenic mice (A-USF). Spleens taken from two phenylhydrazine-treated F 1 females (derived from line II) or wild-type mice were homogenized and subjected to ChIP analysis using antibodies against IgG, RNA Pol II, or USF2. Error bars reflect standard deviations from two independent experiments.

    Journal: Molecular and Cellular Biology

    Article Title: Defective Erythropoiesis in Transgenic Mice Expressing Dominant-Negative Upstream Stimulatory Factor ▿

    doi: 10.1128/MCB.00419-09

    Figure Lengend Snippet: Generation and analysis of mice expressing A-USF. (A) DNA construct pITRp543f2A-USF4 used to generate transgenic mice expressing dominant-negative USF (A-USF). The A-USF coding region is under the control of the human β-globin gene promoter (β-P) and 3′ enhancer (3′E) as well as human LCR elements HS2 and HS3 plus flanking DNA. The DNA construct is flanked on either site by insulator elements derived from the chicken β-globin gene locus (cHS4). (B) SYBR green stain of the RT-PCR analysis of A-USF expression in transgenic (founders I and III and line II F 1 littermates 1 to 3 [II/1 to II/3]) and wild-type (WT) mice. RNA was isolated from the spleens of phenylhydrazine-treated mice, reverse transcribed, subjected to PCR analysis with primers specific to the A-USF coding region, and electrophoresed in 5% Tris-borate-EDTA polyacrylamide gels. (C) Western blot analysis of A-USF expression in transgenic or wild-type mice. Protein was isolated from the spleen or liver of phenylhydrazine-treated mice and subjected to Western blot analysis using an antibody against USF1, which also detects A-USF. (D) qRT-PCR analysis of β maj -globin gene expression in spleens of A-USF transgenic line II F 1 littermates, A-USF founder mouse I, and wild-type mice. Data from the three line II F 1 littermates were combined and are designated II/1/2/3. GAPDH was used as a loading control, and results from samples were normalized to those of the wild type. (E) ChIP analysis of RNA Pol II and USF2 interactions with the β maj -globin gene promoter control mice (WT) and transgenic mice (A-USF). Spleens taken from two phenylhydrazine-treated F 1 females (derived from line II) or wild-type mice were homogenized and subjected to ChIP analysis using antibodies against IgG, RNA Pol II, or USF2. Error bars reflect standard deviations from two independent experiments.

    Article Snippet: RNA was subjected to analysis by quantitative real-time reverse transcription PCR (qRT-PCR) or reverse transcription PCR (RT-PCR) using the MyiQ (Bio-Rad) system, and reactions were carried out using the iQ SYBR green super mix (Bio-Rad). qRT-PCR conditions were the following: 95°C for 5 min, followed by 40 cycles of 94°C for 30 s, 59°C for 30 s, and 72°C for 1 min (readings were taken after every cycle).

    Techniques: Mouse Assay, Expressing, Construct, Transgenic Assay, Dominant Negative Mutation, Derivative Assay, SYBR Green Assay, Staining, Reverse Transcription Polymerase Chain Reaction, Isolation, Polymerase Chain Reaction, Western Blot, Quantitative RT-PCR, Chromatin Immunoprecipitation

    Effects of A-USF expression on the expression of erythroid genes and erythroid cell-specific transcription factors. RNA was extracted from 10.5- or 11.5-dpc embryos, reverse transcribed, and subjected to qRT-PCR performed in triplicate. (A) qRT-PCR analysis of ɛγ-globin, β H1 -globin, β min -globin, Hba α1, USF1 (left; 10.5 dpc), and β maj -globin (right; 11.5 dpc) gene expression in A-USF transgenic (TG II/1 to II/4) and wild-type (WT 1 to 4) mouse embryos. Two sets of four embryos, each containing two TG and two WT animals, were examined. GAPDH was used as an internal control, and sample data were normalized to those for a respective WT littermate. Data are represented as means ± standard errors of the means of at least three PCRs on each sample. (B) qRT-PCR analysis of EKLF, GATA-1, Tal-1, p45, Band3, HoxB4, and USF1 gene expression in the yolk sac of the transgenic (TG II/1 and II/2) and wild-type (WT 1 and 2) 10.5-dpc embryos examined in panel A (left). Data are presented as described for panel A.

    Journal: Molecular and Cellular Biology

    Article Title: Defective Erythropoiesis in Transgenic Mice Expressing Dominant-Negative Upstream Stimulatory Factor ▿

    doi: 10.1128/MCB.00419-09

    Figure Lengend Snippet: Effects of A-USF expression on the expression of erythroid genes and erythroid cell-specific transcription factors. RNA was extracted from 10.5- or 11.5-dpc embryos, reverse transcribed, and subjected to qRT-PCR performed in triplicate. (A) qRT-PCR analysis of ɛγ-globin, β H1 -globin, β min -globin, Hba α1, USF1 (left; 10.5 dpc), and β maj -globin (right; 11.5 dpc) gene expression in A-USF transgenic (TG II/1 to II/4) and wild-type (WT 1 to 4) mouse embryos. Two sets of four embryos, each containing two TG and two WT animals, were examined. GAPDH was used as an internal control, and sample data were normalized to those for a respective WT littermate. Data are represented as means ± standard errors of the means of at least three PCRs on each sample. (B) qRT-PCR analysis of EKLF, GATA-1, Tal-1, p45, Band3, HoxB4, and USF1 gene expression in the yolk sac of the transgenic (TG II/1 and II/2) and wild-type (WT 1 and 2) 10.5-dpc embryos examined in panel A (left). Data are presented as described for panel A.

    Article Snippet: RNA was subjected to analysis by quantitative real-time reverse transcription PCR (qRT-PCR) or reverse transcription PCR (RT-PCR) using the MyiQ (Bio-Rad) system, and reactions were carried out using the iQ SYBR green super mix (Bio-Rad). qRT-PCR conditions were the following: 95°C for 5 min, followed by 40 cycles of 94°C for 30 s, 59°C for 30 s, and 72°C for 1 min (readings were taken after every cycle).

    Techniques: Expressing, Quantitative RT-PCR, Transgenic Assay

    Hepatopancreatic Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time qRT-PCR . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P

    Journal: BMC Molecular Biology

    Article Title: Molecular cloning and tissue expression of the fatty acid-binding protein (Es-FABP) gene in female Chinese mitten crab (Eriocheir sinensis)

    doi: 10.1186/1471-2199-11-71

    Figure Lengend Snippet: Hepatopancreatic Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time qRT-PCR . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P

    Article Snippet: SYBR Green real-time qRT-PCR analysis Real-time qRT-PCR was performed in a C1000™ Thermal Cycler (BioRad CFX 96™ Real-Time System) according to the manufacturer's instructions.

    Techniques: Expressing, Activity Assay, Quantitative RT-PCR

    Ovarian Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time qRT-PCR . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P

    Journal: BMC Molecular Biology

    Article Title: Molecular cloning and tissue expression of the fatty acid-binding protein (Es-FABP) gene in female Chinese mitten crab (Eriocheir sinensis)

    doi: 10.1186/1471-2199-11-71

    Figure Lengend Snippet: Ovarian Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time qRT-PCR . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P

    Article Snippet: SYBR Green real-time qRT-PCR analysis Real-time qRT-PCR was performed in a C1000™ Thermal Cycler (BioRad CFX 96™ Real-Time System) according to the manufacturer's instructions.

    Techniques: Expressing, Activity Assay, Quantitative RT-PCR

    Hemocytes Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time qRT-PCR . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P

    Journal: BMC Molecular Biology

    Article Title: Molecular cloning and tissue expression of the fatty acid-binding protein (Es-FABP) gene in female Chinese mitten crab (Eriocheir sinensis)

    doi: 10.1186/1471-2199-11-71

    Figure Lengend Snippet: Hemocytes Es-FABP expression was influenced by the period of reproductive activity, as determined by real-time qRT-PCR . Es-FABP expression, normalized to beta-actin, was quantified in mitten crab ovaries collected during the stages of rapid ovarian maturation: Stage III-1(Aug), Stage III-1(Sep), Stage III-2 (Sep), Stage III-2 (Oct), Stage III-2 (Nov), and Stage IV (Jan). Bars represent the triplicate mean ± S.E. from three individuals (n = 3). Bars with different letters differed significantly (P

    Article Snippet: SYBR Green real-time qRT-PCR analysis Real-time qRT-PCR was performed in a C1000™ Thermal Cycler (BioRad CFX 96™ Real-Time System) according to the manufacturer's instructions.

    Techniques: Expressing, Activity Assay, Quantitative RT-PCR